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<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P4Y0</accession>
    <name>A0A218P4Y0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein eS17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps17e</name>
        <name evidence="5" type="ORF">A3L08_00065</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853101.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P4Y0"/>
    <dbReference type="GeneID" id="33314617"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00065"/>
    <dbReference type="OrthoDB" id="52479at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.20">
        <property type="entry name" value="Ribosomal protein S17e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00511">
        <property type="entry name" value="Ribosomal_eS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001210">
        <property type="entry name" value="Ribosomal_eS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018273">
        <property type="entry name" value="Ribosomal_eS17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036401">
        <property type="entry name" value="Ribosomal_eS17_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002242">
        <property type="entry name" value="PRK01151.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00833">
        <property type="entry name" value="Ribosomal_S17e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116820">
        <property type="entry name" value="Rps17e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00712">
        <property type="entry name" value="RIBOSOMAL_S17E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ05838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="67" mass="8104" checksum="19C65FFA4A3E04A0" modified="2017-09-27" version="1">MGNIKQTFIKRVARELFDRYPNEFTRDFEHNKKKVEELTTVQSKTIRNRIAGYLTRLVRMKEEGKML</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P506</accession>
    <name>A0A218P506_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">3-dehydroquinate dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_00125</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853113.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P506"/>
    <dbReference type="GeneID" id="33314629"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00125"/>
    <dbReference type="OrthoDB" id="87732at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051782">
        <property type="entry name" value="ABC_Transporter_VariousFunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42939">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN ALBC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42939:SF1">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN ALBC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ05850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="253" mass="27820" checksum="01088BA07EEC5258" modified="2017-09-27" version="1">MIEVENLVKRFGSTVAVNGITFTVNDGEIYGLLGPNGSGKSTTMKILAGILAPTSGKVLVDGVDVSQDPLRVKEMTGYVPETPVLYESLTPIEFFNFVGSVRRIPEEKLQERVDALVKAFGIEEYLGEMIGSLSFGTRQKVSLISAMLHDPAVLILDEAMNGLDPKSARILRELLMDFREDGKSVVFSTHVLALAEMICDRIGVIYNGRLIAEGTVEQLKEFAHEESLEDVFLKLTESRDEVLGIVQALRETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P514</accession>
    <name>A0A218P514_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853122.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P514"/>
    <dbReference type="GeneID" id="33314638"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00170"/>
    <dbReference type="OrthoDB" id="36110at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02042">
        <property type="entry name" value="ParAB_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000285">
        <property type="entry name" value="Sporulation initiation inhibitor Soj"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050678">
        <property type="entry name" value="DNA_Partitioning_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13696:SF99">
        <property type="entry name" value="COBYRINIC ACID AC-DIAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13696">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009320">
        <property type="entry name" value="Nuc_binding_HP_1000"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="4"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="256" mass="28643" checksum="5ACBF9F82C94EADA" modified="2017-09-27" version="1">MAVVISVANQKGGVGKTTLTMNLAYALADMGRKVLLVDIDPQFNLTFGLVGMDVLQYGDRNVGTLMTRDSDIEETIVKVRENIDLIPSHLNLSAKEIEIINTYNRERRLEKALTPILPDYDYVFIDNPPSMGIFLVNSLTASDYVLIPLELSYFGVIGMQLMFNLMRMIREETNENLKLLGLVPNKFTRQTKVPKMRLKELKETYPDAPILTTIPKAIALEKAQSQGVSIFEFEGDGRAAKAFAKLAREVVEIVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P524</accession>
    <name>A0A218P524_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_00275</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853141.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P524"/>
    <dbReference type="GeneID" id="33314659"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00275"/>
    <dbReference type="OrthoDB" id="86024at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ05878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="190" mass="21849" checksum="D312C47BF9A2AF8B" modified="2017-09-27" version="1">MELRIPYIVFKTGTGEYGVDAYFSLRVEKPERRATLIRRTDALERVNAKPPGTLLDAGSVDGYLTSLFMALYDLSGERFNERAHHMRRWSLWRLIGIPTGHQRHVDRDEELAEKSREALLALAIVRKVLGVKAPTELEKVRIIPKGYAVLRLEISGETVSDPVYRELFKIDSNAGMAIQWLRLKTEREER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P550</accession>
    <name>A0A218P550_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sodium:proton antiporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00385</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853162.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P550"/>
    <dbReference type="GeneID" id="33314681"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00385"/>
    <dbReference type="OrthoDB" id="371891at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="80" mass="8749" checksum="EB13B2CA30193158" modified="2017-09-27" version="1">MKKVALLLTAALGFLLLSLTYSPPYEGSYTYYVSHWEEIGVPNLVSAILAGWRAYDSLGEASLLFTAVTGFYILLGGKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P581</accession>
    <name>A0A218P581_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TIGR00266 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_00535</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853190.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P581"/>
    <dbReference type="GeneID" id="33314711"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00535"/>
    <dbReference type="OrthoDB" id="7592at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.160.10">
        <property type="entry name" value="Mitochondrial biogenesis AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002838">
        <property type="entry name" value="AIM24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036983">
        <property type="entry name" value="AIM24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016031">
        <property type="entry name" value="Trp_RNA-bd_attenuator-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00266">
        <property type="entry name" value="TIGR00266 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43657:SF1">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 24, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43657">
        <property type="entry name" value="TRYPTOPHAN RNA-BINDING ATTENUATOR PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01987">
        <property type="entry name" value="AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51219">
        <property type="entry name" value="TRAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ05927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="222" mass="24241" checksum="89149197E3DC72AA" modified="2017-09-27" version="1">MRYSIEHRPSFSLVEIELDSGEAVQAEAGAMVHMSPNIRMETKAKGGVFGALKRSMLGGESFFINTFRAEGGPGTVGFAPSYPGDIEAFELDGTLYAQSGAFLASTEGIDIDTKWGGARTFFGREGLFLLKMSGKGTVFLSSFGAIYKKELRNERFVIDTGHMVAFTEGLDFNVRRVGGLKSTLFSGEGLVAEFYGTGTLYIQTRSLDSFLSWIIPHLPERD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P591</accession>
    <name>A0A218P591_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_00595</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_198362125.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P591"/>
    <dbReference type="GeneID" id="33314723"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00595"/>
    <dbReference type="OrthoDB" id="103681at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007168">
        <property type="entry name" value="Phageshock_PspC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052027">
        <property type="entry name" value="PspC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33885">
        <property type="entry name" value="PHAGE SHOCK PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33885:SF3">
        <property type="entry name" value="PHAGE SHOCK PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04024">
        <property type="entry name" value="PspC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Phage shock protein PspC N-terminal" evidence="7">
        <location>
            <begin position="3"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ05938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="144" mass="15719" checksum="32623F496B72F03A" modified="2017-09-27" version="1">MAKKLVRSKRERVFLGVLGGLAEHLEVDPTLVRLIFIVLLVFNPVAMTLLYFLAALIIPEEGSEGTEEPLSDRIDAVVSETSDRLDEIFSGNENTKAIALVLVLLGALLLAGPFMPLFMPAVNFRTLAALVFLIVGIILLMRGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P596</accession>
    <name>A0A218P596_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sarcosine oxidase subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_00640</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853209.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P596"/>
    <dbReference type="GeneID" id="33314732"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00640"/>
    <dbReference type="OrthoDB" id="168391at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF287">
        <property type="entry name" value="FAD-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="2">
        <location>
            <begin position="12"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ05947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="386" mass="43046" checksum="04E35CA5CDA11F11" modified="2017-09-27" version="1">MPTKTLPEKSEITVIGGGIVGVTIAHELAKRGEEVTVIEKRFIGSGSTFRCGTGIRQQFNDEANVQVMKRSVELWKRYSEEYGFPFEQTGYLFLLYDDEEVEEFKKNIAIQNKFGVPTRLITPEEAKEIVPLLDISEVVAASWNPTDGKADPFHSTAKFALHAEEFGAKLVEYTEVKDFIVENGEIKGLKTSRGTIKTGIVINATNAWAKLVNAMAGIRTKIPIEPYKHQAVITQPIKKGAVKPMVISFRYGHAYLTQTAHGGVVGGVGYEEGPTYDLNPTYEFMRQVSYYFTKIIPALRELLILRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAVAEMVADLITKGKTDLPVEWYDPYRFERGELRGQALQMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5E2</accession>
    <name>A0A218P5E2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Indolepyruvate oxidoreductase subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_00880</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853250.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5E2"/>
    <dbReference type="GeneID" id="33314780"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00880"/>
    <dbReference type="OrthoDB" id="53326at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016903">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052198">
        <property type="entry name" value="IorB_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004915">
        <property type="entry name" value="PRK06274.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43854">
        <property type="entry name" value="INDOLEPYRUVATE OXIDOREDUCTASE SUBUNIT IORB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43854:SF1">
        <property type="entry name" value="INDOLEPYRUVATE OXIDOREDUCTASE SUBUNIT IORB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate/ketoisovalerate oxidoreductase catalytic" evidence="2">
        <location>
            <begin position="11"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ05988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="194" mass="21551" checksum="7E5C11706A380461" modified="2017-09-27" version="1">MEFNLIITGVGGQGGLTLSRIVGNAAMHEGYNVRIGETLGMSQRYGSVLSYLRFGEEVYSPLIEEGEADLMLALEPAEALRNARFLSKKSVAVINAYPIHTATTLVGKERYPELEEIKEAIGKICPVHMANFQEEADKINPRTLGVLMLGYAFGKGLIPLRKESIYEGIKETLREKLWEVNFRAFERGIELAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5I2</accession>
    <name>A0A218P5I2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_01170</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853299.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5I2"/>
    <dbReference type="GeneID" id="33314838"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01170"/>
    <dbReference type="OrthoDB" id="84520at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="entry name" value="PmbA/TldD superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047657">
        <property type="entry name" value="PmbA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43421">
        <property type="entry name" value="METALLOPROTEASE PMBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43421:SF1">
        <property type="entry name" value="METALLOPROTEASE PMBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="Putative modulator of DNA gyrase, PmbA/TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloprotease TldD/E N-terminal" evidence="1">
        <location>
            <begin position="16"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloprotease TldD/E central" evidence="3">
        <location>
            <begin position="109"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloprotease TldD/E C-terminal" evidence="2">
        <location>
            <begin position="219"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="425" mass="46729" checksum="207F8CED106A612E" modified="2017-09-27" version="1">MIDELVAILEGKNVEWEIYWEMGRGGSFKIEREKVERSQRKFYSGVGLRIGYRGRLGFSYITGLNHDRKTLEDFVEKTINLARVSEVPFVGFPEPGKPAAVENLYDRRIKEMPFEEAYSLASDFARLMAELKGASTLSGSLAFGVNTYGLVNSNGVELEGRSTGMSASVYAVLGTGTGSYYQSYRSLQPLEELEKGIREALRDAEMSARAKPIDGHFGEVLLEPDAFQAVLSIFLENVFGDSIYFRRSRFSSPGETVGSECLTILDDATLPAGSGSYPFDGEGVPGKRTVLIEDGVLKSFLLDFTYASFLGMESTGNAVRDFRTKPHIGTSNLLVEPGKESLEDFEGIVVKDVFGEHTANPVSGDFSLTVGLGYVVKNGEIRPFRDNMLSGNVFELLRSVSAVGKRPLRRGSFVSPRVLTSARIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5L5</accession>
    <name>A0A218P5L5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Transcription factor E</fullName>
            <shortName evidence="4">TFE</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">TFIIE subunit alpha homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Transcription initiation factor TFIIE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tfe</name>
        <name evidence="8" type="ORF">A3L08_01355</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Transcription factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Facilitates transcription initiation by enhancing TATA-box recognition by TATA-box-binding protein (Tbp), and transcription factor B (Tfb) and RNA polymerase recruitment. Not absolutely required for transcription in vitro, but particularly important in cases where Tbp or Tfb function is not optimal. It dynamically alters the nucleic acid-binding properties of RNA polymerases by stabilizing the initiation complex and destabilizing elongation complexes. Seems to translocate with the RNA polymerase following initiation and acts by binding to the non template strand of the transcription bubble in elongation complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer. Interaction with RNA polymerase subunits RpoF and RpoE is necessary for Tfe stimulatory transcription activity. Able to interact with Tbp and RNA polymerase in the absence of DNA promoter. Interacts both with the preinitiation and elongation complexes.</text>
    </comment>
    <comment type="domain">
        <text evidence="4">The winged helix domain is involved in binding to DNA in the preinitiation complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TFE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853335.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5L5"/>
    <dbReference type="GeneID" id="33314876"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01355"/>
    <dbReference type="OrthoDB" id="5935at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01909">
        <property type="entry name" value="TFE_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016481">
        <property type="entry name" value="TF_E_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039997">
        <property type="entry name" value="TFE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017919">
        <property type="entry name" value="TFIIE/TFIIEa_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002853">
        <property type="entry name" value="TFIIE_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024550">
        <property type="entry name" value="TFIIEa/SarR/Rpc3_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004910">
        <property type="entry name" value="PRK06266.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00373">
        <property type="entry name" value="transcription factor E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097:SF7">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02002">
        <property type="entry name" value="TFIIE_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006373">
        <property type="entry name" value="TF_E_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00531">
        <property type="entry name" value="TFIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51344">
        <property type="entry name" value="HTH_TFE_IIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH TFE/IIEalpha-type" evidence="7">
        <location>
            <begin position="5"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="NCBIfam" id="TIGR00373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="181" mass="21572" checksum="9ED1798FBC6DF651" modified="2017-09-27" version="1">MGRRKNKELLELAMDMGGEEAVEVIKALEKKKEATDEELAEMTEIRVNTVRKVLYMLYDQGLAEFKRIRDKETGWYYYYWRLETKRLPEILRAKKMAELKRLKEMLEEETSEIYYHCGTPGHPKLTFDEAMEYEFQCPICGQMLMQYDNTAIVEELKKRIEELEIELGLRKKPKARKSESN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5N1</accession>
    <name>A0A218P5N1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0215 protein A3L08_01400</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0215 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853342.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5N1"/>
    <dbReference type="GeneID" id="33314885"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01400"/>
    <dbReference type="OrthoDB" id="15207at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2170.10">
        <property type="entry name" value="archaeoglobus fulgidus dsm 4304 superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00582">
        <property type="entry name" value="UPF0215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002802">
        <property type="entry name" value="Endo_dU"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001977">
        <property type="entry name" value="PRK00766.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39518">
        <property type="entry name" value="UPF0215 PROTEIN MJ1150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39518:SF2">
        <property type="entry name" value="UPF0215 PROTEIN MJ1150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01949">
        <property type="entry name" value="Endo_dU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006380">
        <property type="entry name" value="UCP006380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="195" mass="21765" checksum="DF75D6D7C335E22C" modified="2017-09-27" version="1">MIRKVKPQIRVVGFDDGTFSFSSKLNRERTVLIGVVMKGSQEVVGVLSRWITVDGRDATDAMIDAVLSSRFKDLRVILLKGITYAGFNVVDVERLYRETGLPVVVVVRKKPDLVAMENALRKHFPDAKERVELLRKAPPLLELIPGKLYIQAVGLDEETAREIVRVTTKTGLIPEPLRLAHMIASAVMTGESTRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5N8</accession>
    <name>A0A218P5N8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01470</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853355.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5N8"/>
    <dbReference type="GeneID" id="33314899"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01470"/>
    <dbReference type="OrthoDB" id="89178at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="78" mass="8817" checksum="9FCE2F80393AA8EF" modified="2017-09-27" version="1">MEEPIVIGKDKFRISEDETAKRELRVVKVHDDVIQVQEEVHGIIALVGASSSVNIKKDELKNLIKVAKEEFGWADICE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5P8</accession>
    <name>A0A218P5P8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Type-4 uracil-DNA glycosylase</fullName>
            <ecNumber evidence="3">3.2.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">A3L08_01525</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.</text>
            <dbReference type="EC" id="3.2.2.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the uracil-DNA glycosylase (UDG) superfamily. Type 4 (UDGa) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.27" evidence="3"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853364.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5P8"/>
    <dbReference type="GeneID" id="33314910"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01525"/>
    <dbReference type="OrthoDB" id="8612at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10030">
        <property type="entry name" value="UDG-F4_TTUDGA_SPO1dp_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.470.10:FF:000013">
        <property type="entry name" value="Type-4 uracil-DNA glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053423">
        <property type="entry name" value="Type-4_UDG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051536">
        <property type="entry name" value="UDG_Type-4/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005273">
        <property type="entry name" value="Ura-DNA_glyco_family4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040953">
        <property type="entry name" value="Arch_udg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00758">
        <property type="entry name" value="UDG_fam4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33693:SF1">
        <property type="entry name" value="TYPE-4 URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33693">
        <property type="entry name" value="TYPE-5 URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00987">
        <property type="entry name" value="UreE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="12">
        <location>
            <begin position="31"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="195" mass="22325" checksum="983C2B53EA987645" modified="2017-09-27" version="1">MGNEELMRKLEEKIRNCQKCPLGQLRTNAVPGAGSYDAKVMFVGEAPGYWEDQKGLPFVGRAGKVLDELLEMIGLRREEVYITNIVKCRPPENRDPTEEEIRTCSPYLDRQIDIIRPKVIVPLGRHSMGYILRKFGFEPEPISKIHGKTFEAHTLFGKIIVMPSYHPAAALYRPQIREELRKDFMKLKELIGSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Q1</accession>
    <name>A0A218P5Q1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853379.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5Q1"/>
    <dbReference type="GeneID" id="33314927"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01610"/>
    <dbReference type="OrthoDB" id="97610at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="123" mass="14019" checksum="88AC0AE2ABBF374A" modified="2017-09-27" version="1">MFLHIETGLASEDYEILLKAVKKEMGSTTEKLVELGFDEEVAINTLVFEVPSSSNFYCKACLDAFDDFESAQQHVIEAHSKENVPKFRGKSVKELAGMLVEEYNRIFGDVHFRTKKQRARVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5U2</accession>
    <name>A0A218P5U2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NADH dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_01790</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853410.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5U2"/>
    <dbReference type="GeneID" id="33314963"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01790"/>
    <dbReference type="OrthoDB" id="43567at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="entry name" value="Cytochrome-c3 Hydrogenase, chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052197">
        <property type="entry name" value="ComplexI_49kDa-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43485:SF1">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43485">
        <property type="entry name" value="HYDROGENASE-4 COMPONENT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="HydB/Nqo4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0533">Nickel</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH-quinone oxidoreductase subunit D" evidence="4">
        <location>
            <begin position="122"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="406"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="375"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="378"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601501-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="426" mass="48215" checksum="27FBD8B17BAF3D0D" modified="2017-09-27" version="1">MTEKVEYWVKIPFGPIHPGLEEPEKFILTLDGERIVDVDVKLGYNLRGIQWIALRRNYIQIMYLAERMCGICSFSHNHTYTRAVEEAGGIEVPERAEYIRVIIGELERIHSHLLNLGVLAHDIGYDTLLHLTWLAREKVMDTLEAVSGNRVNYSMITIGGVRRDIDEKKRRIILDMIKYYKEVFPQIEEAFLHDPTIEARFRDTAVISKRVALEQGAVGPTGRGSGIRDDARWSEKLGVYPDLGIKPVMPQDVTGERPRGDVFDRMAVRIGELYQSLELIENALDGMPDGKIKAFPKDNVLYAKLRLMVDGEGIGRYEAPRGELVHYVRGKKGSDKPLRWKPREPTFPNLFAMAKGVIGDQVADFVIAVASIDPCFSCTDRVAVIQNGKKRVLTEKDLLKESIRKTREMNPDIKGDPTPIGASCSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5W6</accession>
    <name>A0A218P5W6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Adenosine monophosphate-protein transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01905</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853432.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5W6"/>
    <dbReference type="GeneID" id="33314986"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01905"/>
    <dbReference type="OrthoDB" id="371841at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1520.10">
        <property type="entry name" value="Ta1353-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007153">
        <property type="entry name" value="Adenosine_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036902">
        <property type="entry name" value="Ta1353-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36155">
        <property type="entry name" value="BLL5354 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36155:SF1">
        <property type="entry name" value="BLL5354 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04008">
        <property type="entry name" value="Adenosine_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103165">
        <property type="entry name" value="Ta1353-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="162" mass="17764" checksum="3A9427DA84CD4F45" modified="2017-09-27" version="1">MVKIEVIDIEKPEGVEVIIGQGNFSIFTVDDLAKTLLTTVPGIKFGVAMNEAKPQLTRFTGNDEELEQLAAKNALKIGAGHVFVILMKNAFPINVLNAVKNHPAVAMVYGASENPFQVIVAETELGRSVLGVVDGKAANRIEDEELKRERRELVEKIGYKFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5X3</accession>
    <name>A0A218P5X3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Water stress and hypersensitive response domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01945</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853440.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5X3"/>
    <dbReference type="GeneID" id="33314994"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01945"/>
    <dbReference type="OrthoDB" id="105458at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009269">
        <property type="term" value="P:response to desiccation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004864">
        <property type="entry name" value="LEA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013990">
        <property type="entry name" value="WHy-dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03168">
        <property type="entry name" value="LEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00769">
        <property type="entry name" value="WHy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117070">
        <property type="entry name" value="LEA14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Water stress and hypersensitive response" evidence="1">
        <location>
            <begin position="165"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="301" mass="32792" checksum="9DDAB10799925298" modified="2017-09-27" version="1">MNLKAAILGLAIAIILWAGYAVYAAYTLHPQLSAQWGYVDEKTTEIWLDAELSKPLLVPASISNLTIEFMGVPIAEAERFDYSPTGRNARLALAVDNKNLVRALVKYFESGQRGTVNVALRGKLLGVIPINTDIHQEISENILGYLNLSAESKDVAGGLVKTPALVGTTFDWVGEEGGNAVLTAHIKLYNPNGFAIPVGNISFEIQANDIKVGHGKTTKGVVIPAKGYATVDVKTYIEEDSLPKVWATHIRNGEVSKVKADVFLDMTFMKQSYRIKLASYEESVETDIMGELNSMLNEAFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P612</accession>
    <name>A0A218P612_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_02175</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853482.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P612"/>
    <dbReference type="GeneID" id="33315040"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02175"/>
    <dbReference type="OrthoDB" id="86197at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="335" mass="37744" checksum="6FC1EC541F78C9B6" modified="2017-09-27" version="1">MKRLVATLFILLLASSLVSAETYTLPGETPDKKPYNNIGILGEVMVDLNVTIVNTAPFPKFVIVNPRYDFNVFRLSGDEGMTSFRVNGGSYHVPSRLDRTSLNYYIGFWVMPYETVVVNFRIPYNNSYLLQTADYQSVCGDSAKITNVTYYVVNGSLEYGGHIEVPDDISTLSCGVMYPQLVNTPKIISTRAMFPMNDGHIKVLKYEGTVTFRLTNAPNRAGVFNTFFAASIPVIFKDARMYNFTPNYTMTYREYMDEFVWKYKGLNPPEKSQPQPLPSMSNMFQLSNTLLTGVSVGTPEIEPPGEAGLDFPVWIIFMGSSVEIKYHVEWTAQGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P630</accession>
    <name>A0A218P630_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF460 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853497.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P630"/>
    <dbReference type="GeneID" id="33315057"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02260"/>
    <dbReference type="OrthoDB" id="15228at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007408">
        <property type="entry name" value="DUF460"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40707:SF1">
        <property type="entry name" value="DUF460 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40707">
        <property type="entry name" value="POSSIBLE NUCLEASE OF RNASE H FOLD, RUVC/YQGF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04312">
        <property type="entry name" value="DUF460"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="419"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="614"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="669" mass="77620" checksum="74FC5A5306B4EF0D" modified="2017-09-27" version="1">MRLILIFGVDVISENPKKFAVVSWFNGKLERKGEFTLYRLIRFIQSKRPDIVAIDSVTELGDDLRRFLRSLPPETKLVQVTGKPGEQRSLQSLAKEHGIRTADRFDPYEEAKLCALLASKGVGYEVLAFEDEVIVKVTRGRSHGKGGWSQDRYRKRVHNLVRDKVRELEDRLRRADIPFDLETEERDYGLARGEFRIYASREELAGLIRPMRGGDVEVRIYPVERAELGFAPLKGEEAVKERKSIIVGIDPGITVGIAAIDLNGRIIALHSERNMPVGEVFRFVSEIGHPVIVATDVSPAPGFVEKIARSFKANLFVPRESLRIEEKNELLRNLGITVDDDHQRDALAAAYKAYLRLKPKLEHIDARLREAGLSKKADEVKALVIQGYNLGEAMQMVSLRERPKVEENRDEQVREAPDLEPYLRRIRELEKRIEMLENENRELREIIKEQRRTIGRLERRIADYDEEVRRKVLRERELEAKVKRIEVLERQLREAKAVIERLGRDLVQVKRMNVVEIRGSAVPLKVMEVLSWRELERIEREVGLRRGDVLFVVNPAGAGRAIAEELVEKRIRALITEKPLPEPVKVVLREAHVPFFLSEELDVKRVDEFAVVERETLENAIAELMERWKKEDEERKAERLLQLVEEYRLERKKELMRKAEEEKEERQKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P654</accession>
    <name>A0A218P654_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Bacteriochlorophyll synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02245</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853494.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P654"/>
    <dbReference type="GeneID" id="33315054"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02245"/>
    <dbReference type="OrthoDB" id="46008at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011777">
        <property type="entry name" value="Geranylgeranyl_Rdtase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050407">
        <property type="entry name" value="Geranylgeranyl_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02032">
        <property type="entry name" value="GG-red-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685:SF18">
        <property type="entry name" value="DIGERANYLGERANYLGLYCEROPHOSPHOLIPID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685">
        <property type="entry name" value="GERANYLGERANYL DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="1">
        <location>
            <begin position="2"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="370" mass="40639" checksum="D2A786AB786DC598" modified="2017-09-27" version="1">MKYDVLIIGGGPAGNYLANLLARDFSVAVVEKKGSFGGKACTGIIGAASYERLGLPEEAVLNKLRGAVFYSRIQSFEIARKTPQAYIVDRKILEKTLAERAVKRGAEYYMATTFRGFKNGRAVLQHLGERLEIEADFYVGADGVSSTVAKAIGAKTNAEFLSGYEVEVVGDFKEDFVEVWVNKDINDEFFMWVAPVNGGLARVGTLGSIEALNRFLRMRMLKPTSIVEFKAGSVGFGWRKPWVRDNVALIGDAALQIKPTTAGGIVFGAICAHALREALLSGRPGDYEKLCGNVKNQISFGLRFRKVFRGMGQEDIEKVFEVLGSDEARAVIEGQADFDDHVKTAKALLRRPKLLAKLIRISPSIVRYLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P672</accession>
    <name>A0A218P672_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853504.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P672"/>
    <dbReference type="GeneID" id="33315065"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02300"/>
    <dbReference type="OrthoDB" id="46222at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22916">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22916:SF3">
        <property type="entry name" value="UDP-GLCNAC:BETAGAL BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="1">
        <location>
            <begin position="7"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="300" mass="35017" checksum="F9FEA802F6A1CDC1" modified="2017-09-27" version="1">MSRPEVSVIIPTYNRAHLLKRAIESVLRQSFDDFELIVVDDASPDNTQEVVESLDDGRIRYIRLKKNSGGPVARNTGIKKARGRFIALLDDDDEWFPHRLEVQIKKFEEIGKEFGVVYGGFYYVSQQDGRILGKRLPKFKGDVYTHLLKENFIGSPTLLIRRECFKKAGLFDPALTSSQDWDMWLRIARYYEFDYVNEIVAKYYVHGRQISFNMKKYIPGRERFISKHKDINKDPRIMSLHLSQMGMLLILGGNPEKGLKYVARSVAMSPFNLENYLKLMELAMDSRSMEYIKKILHFNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P685</accession>
    <name>A0A218P685_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_02630</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853565.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P685"/>
    <dbReference type="GeneID" id="33315132"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02630"/>
    <dbReference type="OrthoDB" id="10500at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012121">
        <property type="entry name" value="ATPase_PP-loop_MJ1599"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052188">
        <property type="entry name" value="Ni-pincer_cofactor_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018317">
        <property type="entry name" value="QueC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43169:SF3">
        <property type="entry name" value="ATPASE, PP-LOOP SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43169">
        <property type="entry name" value="EXSB FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06508">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036668">
        <property type="entry name" value="ATPase_UCP036668"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="276" mass="32052" checksum="77A9EE52A3693434" modified="2017-09-27" version="1">MLKCSLCIHDERTAKIDIIDGKPLCRECQVYLKHPLNREAIRRELEELMEKVDRAVIAYSGGKDSTVALYLAKEVYRVPELEAVMVDHGLMAREAIENARRVAEVLEVPFTLLRYDYSDIFREALLKAQSPCRACSKRTMEKLRKYALRNGYHYIITGHELPFGHHPYRVMKDGIIQVRLLSLMPERERLEILKELPFELPELPGYTTNCLVLGPALERYWEVHGHSFEHRRIAALVRYGLMDREKAESEVAKPVVPEGQKKFVYQKLGLDTLFLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P691</accession>
    <name>A0A218P691_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853532.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P691"/>
    <dbReference type="GeneID" id="33315097"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02460"/>
    <dbReference type="OrthoDB" id="102087at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="7"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="98" mass="11644" checksum="678443AA480F647D" modified="2017-09-27" version="1">MIKDRICREYLEEIERLERSVVELEEQINELKMQLRLKVDEANSLAVENASLRHKLELMEKRERALINLLRSLKVPLVLIDEERFEDVEVDLGPDSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P693</accession>
    <name>A0A218P693_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Probable dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Dihydroorotate oxidase B, electron transfer subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">pyrK</name>
        <name evidence="14" type="ORF">A3L08_02645</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Responsible for channeling the electrons from the oxidation of dihydroorotate from the FMN redox center in the PyrD type B subunit to the ultimate electron acceptor NAD(+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="11">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="11">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="12">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (NAD(+) route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterotetramer of 2 PyrK and 2 PyrD type B subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the PyrK family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853568.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P693"/>
    <dbReference type="GeneID" id="33315135"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02645"/>
    <dbReference type="OrthoDB" id="35401at2157"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00945"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06220">
        <property type="entry name" value="DHOD_e_trans_like2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.240.10">
        <property type="entry name" value="Dihydroorotate dehydrogenase, electron transfer subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01211">
        <property type="entry name" value="DHODB_Fe_S_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012165">
        <property type="entry name" value="Cyt_c3_hydrogenase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037117">
        <property type="entry name" value="Dihydroorotate_DH_ele_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019480">
        <property type="entry name" value="Dihydroorotate_DH_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023455">
        <property type="entry name" value="Dihydroorotate_DHASE_ETsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050353">
        <property type="entry name" value="PyrK_electron_transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000796">
        <property type="entry name" value="PRK00054.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43513">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE B (NAD(+)), ELECTRON TRANSFER SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43513:SF3">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE B (NAD(+)), ELECTRON TRANSFER SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10418">
        <property type="entry name" value="DHODB_Fe-S_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006816">
        <property type="entry name" value="Cyc3_hyd_g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00409">
        <property type="entry name" value="PHDIOXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11 12" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="8 11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 11" id="KW-0274">FAD</keyword>
    <keyword evidence="3 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 11 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="13">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR006816-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ06306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="233" mass="25820" checksum="81693738C11E4DD9" modified="2017-09-27" version="1">MLKRVELVETWEVAKDVRAFRFDERLKFRPGQFLMVWLPGAGEKPFSLAGEDIIVVKRVGPFTSRLFELGEGDYVWIRGPYGNGFEARGKRVALVAGGIGIPPLYALARKGGGAFEHVTLIYGARSKDELALMDVEDYVDELIITTDDGSAGRKGFPTDVLAERKGEFDQVYACGPEPMLRAVLRVMDYEKVQISAERYMKCGIGVCGSCNLGKYLVCRDGPVFEGPQLRGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P699</accession>
    <name>A0A218P699_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CARDB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_02710</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853581.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P699"/>
    <dbReference type="GeneID" id="33315148"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02710"/>
    <dbReference type="OrthoDB" id="86198at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="483"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="441"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="507"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="451"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="508"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="530"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="543" mass="60246" checksum="BE8692E421235405" modified="2017-09-27" version="1">MRKVVPVFILLLLVISMLPRVSGQFDQLETSDEITILRDDYSSGMIWLTNAGGLTYKVVSYQRFWVEDSEGNKIPGFEFNISPSVFTDWSPKGRYSFSYNISCPSNVSGGTYTLYMRFLAFTPDGLMYILFARIPLHVIAEPLNFGVATAYVQSRPSSSYVLNGEKIVVFSHVANIGHRDVPAVGSVSFVMNGKTYFSDSRNLTLVPGDNLVKFEIPVGYDLPEGSYRVNYRIRYSGGEYTYSKDFQVKFGVTLVGVSLKSGEVSVNEENTAYLTLLSERVIDLNLTVEAYRGGELVSKATKPVRVVRGTTVLEASLPTNVSGNITALLKLTYGERLIGEDNVTYTVSAPLVLKNVSYERTSENEVTFRLTLWNPSESEVDGILTYRISTDDGVLYKDSIKQSIPSGSSEVTVKFEIPVGKTVYYEFALVSAGESSSIKGQFYLEPPAPPTTTTRPPTTTTPSTTTSSNTTTAAGGGGSRETWIALVLVAFILLAVGTWYYLREQGSTKKRVRPKPKRRSPLGRFKRPKKPEFKENKELPKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6A9</accession>
    <name>A0A218P6A9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Serpin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_02765</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853591.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6A9"/>
    <dbReference type="GeneID" id="33315159"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02765"/>
    <dbReference type="OrthoDB" id="371710at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19590">
        <property type="entry name" value="serpin_thermopin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023795">
        <property type="entry name" value="Serpin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF211">
        <property type="entry name" value="GH10112P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serpin" evidence="3">
        <location>
            <begin position="63"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="420" mass="46660" checksum="29C56DE46F502F0B" modified="2017-09-27" version="1">MRRLVLLAILLTVIVGGCLGQAENGKPSPTSSSSTFTPPGTKYDVLKEGQEAPIVRGINAFAVDLYRELAGGEDNLFFSPYSVETALAMAYDGASGATREEMGKVLHLPSENDTRWVGFRYLLLSLKTSNGSPYVLRSANALWIQRDYPIDEKYLWVIRTFYLGRVDKVDFVNDPEGSAREINDWVEEQTNGRIKDILSKLSPDTRLIITNAIYFKGNWSSRFEASETRNGTFHSPSGPVTVPMMHQRGKFPYFENNELQALELPYEGGRLSMLIILPREGKFEKVENELSTEFIEDIMNGTTEESVKVTLPKFRVAQEYHLRDILADMGMGSAFKVPGFSGISPGGNLVISDVLHKTLISVAENGTEAAAVTAVTMTLAAVPGQENFKVFNADRPFIFLIYDRETGAILFMGRLVNPKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6B8</accession>
    <name>A0A218P6B8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide transporter, OPT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_02780</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853594.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6B8"/>
    <dbReference type="GeneID" id="33315162"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02780"/>
    <dbReference type="OrthoDB" id="169602at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004814">
        <property type="entry name" value="Oligopep_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045035">
        <property type="entry name" value="YSL-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00733">
        <property type="entry name" value="OPT family oligopeptide transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645:SF0">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="338"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="371"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="396"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="436"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="497"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="529"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="569"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="598"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="624" mass="64969" checksum="5FA238B941ABDE30" modified="2017-09-27" version="1">MEFKPYIPPEKSLPEYTVKAFVLGVLLSIVMGAANAYLGMYAGMTVSASIPAAVISMALLFAFKDRNILENNMVQTAASAGESLAAGVIFTFPALIVLEYYTTFPYYIVTIIAALGGALGALFTIVLRRAFIAEEKLPYPEGTACAEVLIAGDKGGSHAKPILWGGIFGGVFKLFGSSGLWAGTVEAAKAVGSRVYYIGSDLSAALVAVGYIVGLNIAFLVFLGGAIAWFIAIPIYAGQMGHTDLSPLDLAWTIWSTKIRYMGVGAMVVGGLWSLIKLRGPISRGIRAGLEAAKHRQAGGTLPRTEEDLPLNYVLMLIAAFVIPLFLLYAHILDSIGIAAIMAVIMLILGFFGSAIAGYLAGVVGSSNNPVSGITIMSLLFTALILKALGLSGTEGMVATILVAAVVCTAAAIAGDTMQDLATGYLVGATPKRQQVFEVIGVFAAAFVMAPVLNLLIQAYGIAGTPTAKENALAAPQAFLMAKVTEGVFTGNLEWNMIYIGAGIAIVLIILDEILAMKGSKFRTPVMPVAVGIYLPLSLGVPIFLGGLIRHFVTKARGDSAENPTDPGVLGAAGLIAGEALMGITFAALIVAGVAPSIGFSSNLLGVILLAGIATWLYITGRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6C7</accession>
    <name>A0A218P6C7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02855</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853608.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6C7"/>
    <dbReference type="GeneID" id="33315177"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02855"/>
    <dbReference type="OrthoDB" id="85827at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="258" mass="29620" checksum="E52C06DB0D0D3A86" modified="2017-09-27" version="1">MRVFIRDYLIPWLLLILVWVAIWIFVPGEEKNLSLPNVLSVLILLPLFLLVVLYFVGKTLERYGYSRKDVRRLPEIIEKTHGRLYLSREIFDTIGQALIFWALFSTVIFMTEDPLWGVANAVAMFAWIFAFFVLLVSMVIWVLGFLPALYRLLTGRKLNRDFLVEMMKFNLVSTAILIVVRLIALHVGDVSAPHYVMKLIAFGRNDRIVNSLLELSALNFLFGLVGLYGPKRIGKAAALLLTLIVFGQLWVTWKLLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6D7</accession>
    <name>A0A218P6D7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_02910</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853617.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6D7"/>
    <dbReference type="GeneID" id="33315188"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02910"/>
    <dbReference type="OrthoDB" id="85174at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="102" mass="11634" checksum="F37F9926E22C56B8" modified="2017-09-27" version="1">MERELRRILGEDLANYLELMRAKLAFAEELYGIKMNYVPLITEGEIVVLDKNDGKIKWLRTKSPLTVDELKSLAEKIKKNLESGYVEMLLAMNMSCVNGPGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6E3</accession>
    <name>A0A218P6E3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Potassium transporter Kef</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853609.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6E3"/>
    <dbReference type="GeneID" id="33315178"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02860"/>
    <dbReference type="OrthoDB" id="94794at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="287"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="319" mass="35676" checksum="2A0F0D2858BC1BDF" modified="2017-09-27" version="1">MGRNELFLVFLMGVVFLAMLFSYSLSLSPVESLSFAVILALLYTSTRVFWEKSDKWRGKSGLLKSKGARYLIFFLSSFGFAVLLFGLTYLVLAKPGTSFVSLVKVLGVLFAIGSSLMLLASMFYNRDRTPEKITYSWRNFLRELSASILLFTIAYFSGVSLEKSASMALYVFVATNWYYSTMAHRYVIPEQMLKLRAVINFAAIASGLYLFVIDNALVSGLVGALFAVAEEKDYGITRKLIETGLLERRYAESGVGGLFYAVSYGFGAMVALIVVTGNCNASFIRDFLLTMFRLLYLFTVIFLPFGTFLGWARLKVHGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6G3</accession>
    <name>A0A218P6G3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1616 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_03015</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853636.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6G3"/>
    <dbReference type="GeneID" id="33315209"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03015"/>
    <dbReference type="OrthoDB" id="82282at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011674">
        <property type="entry name" value="DUF1616"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014495">
        <property type="entry name" value="UCP018671"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07760">
        <property type="entry name" value="DUF1616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018671">
        <property type="entry name" value="UCP018671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1616" evidence="2">
        <location>
            <begin position="15"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="333" mass="37650" checksum="9DD2FA919AC04A43" modified="2017-09-27" version="1">MGNPQGFKKYWDLLTVIALSVVLDLLIAFFPDSLARKALGLAFVLFFPGYVFITALFPNRKELDNLERLALSFGLSIAIVPLIGLALNYTPWGIRLIPILISLTVFNIALAVVAIYRRARAFEPWIPWITIERIKKELEWEESSKLDKALTVILIIAILTSIGTLGYVITHPKPGEKFTEFYILGPDGKADNYPTELKVSQNGTLIIGIVNHEGRNVTYYVQIWLVNLTWDNSTNTTIIHEMYPIPGWFNVTLPHVPVDIEGNWTPQFEENYTFSINKPGRWQVWFLLFKDGQPELPPAPPDGNYAETEAKNLILEAVNGTIQSLKLNVEVKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6J7</accession>
    <name>A0A218P6J7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03185</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_198362132.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6J7"/>
    <dbReference type="GeneID" id="33315243"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03185"/>
    <dbReference type="OrthoDB" id="85850at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="109" mass="12826" checksum="DB0023F99D5644CF" modified="2017-09-27" version="1">MQYQGFQGGRAKPSAESVESFGFDELLYEILKEGLFWAALGRSSEVMPFLRGKLLENGVSLEKQRREYMEYLLDELEHFYRRVSWSGEISEAHWKALKSFHRELLALLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6K3</accession>
    <name>A0A218P6K3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="7">ATase</shortName>
            <ecNumber evidence="7">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
            <shortName evidence="7">GPATase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">purF</name>
        <name evidence="13" type="ORF">A3L08_03230</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + L-glutamine + H2O</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7 10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="7 11">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7 8">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="7"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853670.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6K3"/>
    <dbReference type="GeneID" id="33315252"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03230"/>
    <dbReference type="OrthoDB" id="5976at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01931">
        <property type="entry name" value="PurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01134">
        <property type="entry name" value="purF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11907">
        <property type="entry name" value="AMIDOPHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3 7 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 11" id="KW-0408">Iron</keyword>
    <keyword evidence="7 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="12">
        <location>
            <begin position="5"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7 9">
        <location>
            <position position="5"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <position position="348"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="385"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="434"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="437"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="449" mass="49766" checksum="167B373D97443646" modified="2017-09-27" version="1">MREKCGIFATLSENAPKKAYYALIALQHRGQESAGISVWRHRIRTVSGPGLVQDVFKGPVLAKLKSNLAIAHVRYSTSGSLTETQPLETECCGKRIAVAHNGTLTNFLPLRREYENRGIKFHHSVDSELLGISFLWHLKETGDEFEAMKAVFDEVKGAYSVAFLFDGKILVARDPVGFRPLSYGRGDGHYFASEDSALRLFVDESEGGEIRDVEPGEAFLLSEDGVENRVLAKEEHHGCVFEYIYFARPDSTIDGINVYSARVRMGVELARESPAEGDVVIAVPDSGRAAALGFSMESGIPYAEGLIKNRYIGRTFITPGQFNRELKVRLKLSPVREVVSGKRVVLVDDSIVRGTTMRRIVAMLRKAGAREVHVRIASPPIRHPCYMGIDIPTRHELIAAFGSVEKVREAIGADSLAYLSVEGLKRAVGREELCMACLTGDYPEWAFRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6K5</accession>
    <name>A0A218P6K5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Phosphoribosylaminoimidazole-succinocarboxamide synthase</fullName>
            <ecNumber evidence="3 11">6.3.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 11">SAICAR synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">purC</name>
        <name evidence="13" type="ORF">A3L08_03235</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate + ATP = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:22628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the SAICAR synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.6" evidence="3 11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6K5"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03235"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00131"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01415">
        <property type="entry name" value="SAICAR_synt_PurC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000086">
        <property type="entry name" value="Phosphoribosylaminoimidazole-succinocarboxamide synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000006">
        <property type="entry name" value="Phosphoribosylaminoimidazole-succinocarboxamide synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00137">
        <property type="entry name" value="SAICAR_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028923">
        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033934">
        <property type="entry name" value="SAICAR_synt_PurC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001636">
        <property type="entry name" value="SAICAR_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050089">
        <property type="entry name" value="SAICAR_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018236">
        <property type="entry name" value="SAICAR_synthetase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00081">
        <property type="entry name" value="purC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43599">
        <property type="entry name" value="MULTIFUNCTIONAL PROTEIN ADE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43599:SF3">
        <property type="entry name" value="SI:DKEY-6E2.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01259">
        <property type="entry name" value="SAICAR_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01057">
        <property type="entry name" value="SAICAR_SYNTHETASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01058">
        <property type="entry name" value="SAICAR_SYNTHETASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAICAR synthetase/ADE2 N-terminal" evidence="12">
        <location>
            <begin position="7"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="239" mass="27235" checksum="87F68E59F427B940" modified="2017-09-27" version="1">MVKPMQAYEGKAKKIIPLDDGKVVMEFKDDATAFDGGKKAQFKGKGWLNAQISAHLFRVLEEKGIKTHFIGVAGDNKLIVEKLDMYPLEVVVRNVVAGSLKKRLPLDEGTELKEPIVELYYKDDSLHDPMINYYHAKVLGIDESEVKEMERIALKVNEILREYFRERGIILVDFKLEFGKNGRGEIVLGDEISPDTCRFWDAKTGKSLDKDVFRFDKGDLVKAYEELYARLTGETPSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6M3</accession>
    <name>A0A218P6M3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TonB-dependent receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03295</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853682.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6M3"/>
    <dbReference type="GeneID" id="33315265"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03295"/>
    <dbReference type="OrthoDB" id="86278at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0675">Receptor</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="91" mass="10541" checksum="FDC5D733BF2F0ED2" modified="2017-09-27" version="1">MGHTVYYRTIIDRWNEFRDFLEAVCEGLGFRFVEGEDSVMILPECRGVEPLEIKKNGKGFVKTNLVEPCHSIYLLILHSVAFFGSVELWED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6M6</accession>
    <name>A0A218P6M6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6M6"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03395"/>
    <dbReference type="OrthoDB" id="86285at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="319" mass="34111" checksum="4701707C8EBEF162" modified="2017-09-27" version="1">MKMAFFFVALVFGALLVVGSSGNFKEYNSTREVAVTVVPHDREYMGFVCERGYAAVITVSSNSEQDFNALTVMNYLPEDKDVDIALSPDYSGLPGGLGVSIETDDGAPRTLAPSEEYTFLGHVSSGNVEPGEYIIPMDMYATWDGGGASISACPIKLIVVNRPRIEKILLSGNTSDIPMKTFQKWTFQIVVTNPTGEDLDLTIADTIPAEFNVSLGETGASSGTYSFHPANGGGHGHSMPATKMEWDVTVPAGGSEHLNVTIFTRVNGGNQQEFTSCGDYSLNNGAEIKGYGIVSNPLWVSVACCDDNEDDEQCDGHGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6M9</accession>
    <name>A0A218P6M9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pyridoxal 5'-phosphate synthase subunit PdxT</fullName>
            <ecNumber evidence="7">4.3.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pdx2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Pyridoxal 5'-phosphate synthase glutaminase subunit</fullName>
            <ecNumber evidence="7">3.5.1.2</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pdxT</name>
        <name evidence="10" type="ORF">A3L08_03220</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 12">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + phosphate + 3 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the glutaminase PdxT/SNO family.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="7"/>
    <dbReference type="EC" id="3.5.1.2" evidence="7"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853669.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6M9"/>
    <dbReference type="GeneID" id="33315250"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03220"/>
    <dbReference type="OrthoDB" id="26717at2157"/>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903600">
        <property type="term" value="C:glutaminase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006543">
        <property type="term" value="P:L-glutamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008614">
        <property type="term" value="P:pyridoxine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01749">
        <property type="entry name" value="GATase1_PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000041">
        <property type="entry name" value="Glutamine amidotransferase subunit pdxT, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01615">
        <property type="entry name" value="PdxT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002161">
        <property type="entry name" value="PdxT/SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021196">
        <property type="entry name" value="PdxT/SNO_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03800">
        <property type="entry name" value="PLP_synth_Pdx2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559:SF0">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01174">
        <property type="entry name" value="SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005639">
        <property type="entry name" value="Glut_amidoT_SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01236">
        <property type="entry name" value="PDXT_SNO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51130">
        <property type="entry name" value="PDXT_SNO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="7 8">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <begin position="53"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <begin position="142"/>
            <end position="143"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR005639-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR005639-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="197" mass="21595" checksum="D0783A250C8BC854" modified="2017-09-27" version="1">MVKVGVIGLQGDVSEHIEAAKKALENLGVSGEVIWLKKPEQLEGVSAIIIPGGESTTISRLMVKNGLFEPVKKLGEEGLPIMGTCAGLIMLSKEIIGATPEQKFLELLDVKVNRNAYGRQVDSFEAPIKLAFSDEPVIGVFIRAPRIVELLSDKVKPIAWLGDRVVGVEQDNIIGLEFHPELTDDTRVHEYFLRKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6N7</accession>
    <name>A0A218P6N7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein eS24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps24e</name>
        <name evidence="6" type="ORF">A3L08_03365</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the eukaryotic ribosomal protein eS24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853695.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6N7"/>
    <dbReference type="GeneID" id="33315279"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03365"/>
    <dbReference type="OrthoDB" id="27533at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00545">
        <property type="entry name" value="Ribosomal_eS24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001976">
        <property type="entry name" value="Ribosomal_eS24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018098">
        <property type="entry name" value="Ribosomal_eS24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10496">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01282">
        <property type="entry name" value="Ribosomal_S24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00529">
        <property type="entry name" value="RIBOSOMAL_S24E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="98" mass="11469" checksum="246BEEF2975A1E70" modified="2017-09-27" version="1">MEIKVTEIRENKLLGRKEIYFDVIHEGEPTPSRADVKGKLVAMLDLNPETVVVQYIRSYFGSRVSKGYAKAYESRERMLYIEPEYILVRDGLIEKQEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6R6</accession>
    <name>A0A218P6R6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">polynucleotide 5'-hydroxyl-kinase</fullName>
            <ecNumber evidence="2">2.7.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_03600</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of both single-stranded RNA (ssRNA) and single-stranded DNA (ssDNA). Exhibits a strong preference for ssRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 5'-end dephospho-2'-deoxyribonucleoside-DNA + ATP = a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15669"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:13184"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:136416"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.78"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 5'-end dephospho-ribonucleoside-RNA + ATP = a 5'-end 5'-phospho-ribonucleoside-RNA + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54580"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:15179"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.78"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="2.7.1.78" evidence="2"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853734.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6R6"/>
    <dbReference type="GeneID" id="33315325"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03600"/>
    <dbReference type="OrthoDB" id="359472at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051734">
        <property type="term" value="F:ATP-dependent polynucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045116">
        <property type="entry name" value="Clp1/Grc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032319">
        <property type="entry name" value="CLP1_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755">
        <property type="entry name" value="CLEAVAGE/POLYADENYLATION FACTOR IA SUBUNIT CLP1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755:SF3">
        <property type="entry name" value="POLYNUCLEOTIDE 5'-HYDROXYL-KINASE NOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16575">
        <property type="entry name" value="CLP1_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Clp1 P-loop" evidence="10">
        <location>
            <begin position="34"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="355" mass="38811" checksum="D318AF7187AE7E26" modified="2017-09-27" version="1">MNKATYTTDVPGDRLSLLETLSSRQRPTKVMLIGGTDSGKTTLLTFLANGLIERGLRVAIVDSDVGQKGVLPPAAISLAFPEGPFSNPSELRGAAHYFIGTTSPGQYAGEMAVGVKRLVDIAIKSADIVLIDTTGFVTGPGAEMKRLKAELVRPDIVVFLEKGGELEHLRNLLAPYGEILRLPVSPKVRAYSREERREIRGKKWRAYFAASTPVDVDLREVIPGGTSLFQGRPLTPEEVELLSSAFGWLVLAGWKGGRYTVVKSDVESSPRHYDRFALHAVDFEKLSNLLVGFIDGDGLCLGVGILKWISFSARKAQVLTPLSPEEVSKAVELRFGRIRVLENGEELGLLRRDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6S4</accession>
    <name>A0A218P6S4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PRC-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03585</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853731.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6S4"/>
    <dbReference type="GeneID" id="33315322"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03585"/>
    <dbReference type="OrthoDB" id="85839at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.240">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="94"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="218" mass="25336" checksum="61E25FC3D349E151" modified="2017-09-27" version="1">MSEASEAKANQLINKFVISLTEGKILGYVTDINVEVEGDQFYFILKMREVENLGKGQSMFSSEKKLKIKPSDIVNVGPDVIILGNGKVPPLREIERLNQVAEEYNTLVRELEEKDKMIEKLKAENYSLTKQVDELQRELRKFQVMKDDFEHLKEQLVRQEGQLDMAKEYIRLLEGLRHDIDKIKEDVDRLLQNQLEEVVRSIINEELNARGLKKTSFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6T3</accession>
    <name>A0A218P6T3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Xylose isomerase-like TIM barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853747.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6T3"/>
    <dbReference type="GeneID" id="33315341"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03680"/>
    <dbReference type="OrthoDB" id="33250at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00019">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.150:FF:000017">
        <property type="entry name" value="Endonuclease IV related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445:SF0">
        <property type="entry name" value="APURINIC-APYRIMIDINIC ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445">
        <property type="entry name" value="ENDONUCLEASE IV ENDODEOXYRIBONUCLEASE IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylose isomerase-like TIM barrel" evidence="1">
        <location>
            <begin position="28"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="281" mass="32185" checksum="A105497C9B861520" modified="2017-09-27" version="1">MFKVDRLRFGTAGIPLSTPKRSTIDGVAHVRNLGLDAMELEFVRGVNLKPELAKKIKYVAKKHDVLLTAHAPYYINLNAAEKAKVEASKNRIIQSAERLHDAGGWSVVFHAGYYLKQPPQSVYQKILEALRDIEKKLMDKGIKVWIRPELTGKPTQFGDLEEIVKLSEELEMVLPTIDFAHAHARSKGEFNSIEEWREMLSLLEDRLGREALDNMHIHMSGIEYTAKGERRHLNLQESDMKWEDLLKVLKEFKVKGVVISESPNIEEDALLMKKKYEEIEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6W4</accession>
    <name>A0A218P6W4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ASCH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853786.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6W4"/>
    <dbReference type="GeneID" id="33315381"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03880"/>
    <dbReference type="OrthoDB" id="84651at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.30">
        <property type="entry name" value="Hypothetical protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007374">
        <property type="entry name" value="ASCH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04266">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01022">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ASCH" evidence="1">
        <location>
            <begin position="5"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="174" mass="20458" checksum="279B3D96F5E23F05" modified="2017-09-27" version="1">MEHVIALHQVYAELIFRGLKTVEIRKSRAFQEGDLVFLYVARGNPYELRDTLRRLGLHEEQTLTQRGTIAGGFEVGEVIKADLDTLWEMTKETSGLTLVHGENGKKWLGEYIREYGYAFTIERPFLFKEPMSREEMKERYGIHVEGIIHLSRRTRKEWVKNLIEDLLTREVVYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6X1</accession>
    <name>A0A218P6X1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Translation initiation factor 2 subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 9">aIF2-beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8 9">eIF-2-beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">eif2b</name>
        <name evidence="11" type="ORF">A3L08_03920</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853793.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6X1"/>
    <dbReference type="GeneID" id="33315389"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03920"/>
    <dbReference type="OrthoDB" id="38099at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.170:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00232">
        <property type="entry name" value="eIF_2_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004458">
        <property type="entry name" value="TIF2_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00311">
        <property type="entry name" value="aIF-2beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003067">
        <property type="entry name" value="PRK03988.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 11" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation initiation factor IF2/IF5" evidence="10">
        <location>
            <begin position="30"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="143" mass="16293" checksum="B7777BF50ED78699" modified="2017-09-27" version="1">MSEKKVDFYDFEGLLDKAYEELPENVKHHASRFEVPPAQVTIAGNRTIIENFVDIAEAMNRDPSHLLKFILREVATAGTLEGRRVILQGRFTPYLIANKMKKYLKEFVICPVCGSPDTKIIKKGRFHFLKCEACGAETPIQHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6X3</accession>
    <name>A0A218P6X3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">nicotinamidase</fullName>
            <ecNumber evidence="6">3.5.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Nicotinamide deamidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_03795</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; nicotinate biosynthesis; nicotinate from nicotinamide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the isochorismatase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.19" evidence="6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853769.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6X3"/>
    <dbReference type="GeneID" id="33315364"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03795"/>
    <dbReference type="OrthoDB" id="9194at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008936">
        <property type="term" value="F:nicotinamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019363">
        <property type="term" value="P:pyridine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01011">
        <property type="entry name" value="nicotinamidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052347">
        <property type="entry name" value="Isochorismatase_Nicotinamidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11080:SF2">
        <property type="entry name" value="LD05707P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11080">
        <property type="entry name" value="PYRAZINAMIDASE/NICOTINAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="8">
        <location>
            <begin position="5"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="178" mass="19808" checksum="CFD0A3ACEDAB3804" modified="2017-09-27" version="1">MPEEALIVVDMQRDFMPGGALPVPDGDRIIPWCNHYIEEFRKRGALIVATRDWHPENHISFKEQGGPWPAHCVRNTPGAEFVVDLPSDAVIISKATEPDKEAYSGFEGTELAEILRKNGVKRVYVCGVATEYCVKATALDAIKHGFETYLLSDAVKGINPEDEEKALREMERAGVKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Z3</accession>
    <name>A0A218P6Z3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATP-dependent DNA ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_04035</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853813.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Z3"/>
    <dbReference type="GeneID" id="33315411"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04035"/>
    <dbReference type="OrthoDB" id="14524at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07894">
        <property type="entry name" value="Adenylation_RNA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041596">
        <property type="entry name" value="Lig_Pab1020_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021122">
        <property type="entry name" value="RNA_ligase_dom_REL/Rnl2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001072">
        <property type="entry name" value="RNA_ligase_Pab1020"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01209">
        <property type="entry name" value="RNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18330">
        <property type="entry name" value="Lig_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09414">
        <property type="entry name" value="RNA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01048">
        <property type="entry name" value="Y414FAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA ligase" evidence="1">
        <location>
            <begin position="86"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="RNA ligase Pab1020 C-terminal" evidence="2">
        <location>
            <begin position="255"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="380" mass="43993" checksum="7DF39D755F7E7098" modified="2017-09-27" version="1">MVSSHFMSLLLKIGVPEDRLLVLEGKGGIVEDEFEGIRYVRFRDSAKGFRRGTVVFENGEAVLGFPHIKRVVQLENGVKRVFKSRPFYVEEKVDGYNVRVVKVEDRVLALTRGGFVCPFTTERIEDFVNFEFFKDYPNLVLAGEMAGPESPYIVEGPPYVKEDIEFFLFDIQEKGTGKSLPVEERYKLAEEYGIPQVERFGLFDRSRLDELYDLIERLGREKREGIVMKSPDMRKIAKYVTPYANINDIKIGSHIFFDLPHGYFMGRIERLAFYLAEKHVKGEEFDEYAKALGKALLRSFVESIHEVANGGEVEETFTVRVKNISTAHRMVTHFERLGVNIHIEDIEDLKNGYWRITFKRVYPDATREIKELWNGLAFVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P732</accession>
    <name>A0A218P732_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Small ribosomal subunit protein uS2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rps2</name>
        <name evidence="7" type="ORF">A3L08_04275</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853853.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P732"/>
    <dbReference type="GeneID" id="33315459"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04275"/>
    <dbReference type="OrthoDB" id="371797at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000030">
        <property type="entry name" value="30S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_A">
        <property type="entry name" value="Ribosomal_uS2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023454">
        <property type="entry name" value="Ribosomal_uS2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_uS2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005707">
        <property type="entry name" value="Ribosomal_uS2_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01012">
        <property type="entry name" value="uS2_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11489">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="201" mass="23071" checksum="F7458E91B63C1584" modified="2017-09-27" version="1">MEEYLVPLDQYLAAGVHIGTQQKTQDMKKFIYRVRQDGLYVLDVRKTDERLRVAGRFLAKFDPESILAVSVRLYGQKPVKKFGEVTGAKAIPGRFLPGTMTNPKVKKFMEPDVLIVTDPRADHQAMKEAIEIGIPIVALVDTENFLSYVDLAIPTNNKGRKALALIYWILAREILYNRKEIESREDFKVPVEDFEMRIIRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P734</accession>
    <name>A0A218P734_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphopyruvate hydratase</fullName>
            <ecNumber evidence="3">4.2.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_04270</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="3"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853852.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P734"/>
    <dbReference type="GeneID" id="33315458"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04270"/>
    <dbReference type="OrthoDB" id="85319at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006223">
        <property type="entry name" value="PRK08350.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF1">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enolase N-terminal" evidence="8">
        <location>
            <begin position="4"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="7">
        <location>
            <begin position="124"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="341" mass="37320" checksum="8B82CF6BF9C52D1B" modified="2017-09-27" version="1">MTVIENVIGRVAVLRGGRYSVEVDVITSSGFGRFAAPIDENPSLYIAEAHRAVSEVDEIIGPELIGFDASEQELIDSYLWEIDGTEDFSHIGANTALAVSVAVAKAAASVKKTPLYSYLGGTFTTELPVPILEVASGETFDYYVTVRDLMEITDVIDALNKLLEHAEGNSVGAFSKATEKATDELGLDVALGLVQKKPLGTEEVLSIVEDNNVAYIKPIGDEELFLELIAGTHEVFVDGEYLFREKDILDRRYYNALSIKPINLGTLTDLYNLVNDAKSERITPILAEARYESSDEALAHIAVGLRCPAMVFRRDSIAKLNELIRIAEDLGERGRIITFEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P763</accession>
    <name>A0A218P763_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">X-Pro aminopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_04450</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M24B family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853880.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P763"/>
    <dbReference type="GeneID" id="33315494"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04450"/>
    <dbReference type="OrthoDB" id="1346at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01092">
        <property type="entry name" value="APP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050659">
        <property type="entry name" value="Peptidase_M24B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46112">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46112:SF9">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase N-terminal" evidence="5">
        <location>
            <begin position="2"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24" evidence="4">
        <location>
            <begin position="141"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="358" mass="40312" checksum="857054C25E96D3A4" modified="2017-09-27" version="1">MRLDRFVSLMKERGFDGALISPGTNLYYLTGLHIHEAGERPTVLVVSSDGNYRLLAPSLYENVVRNFPATFWRDGENPYEKLAWILAEFKLSSGKLLVEDTMRADWLINIFRVGNRGFEFYPLSSVIRELRMRKDEKEIDYMKHAAKVVDRVFEELLSWDLLGMRESELALKIELAVRELSDGISFEPIVASGENAANPHHAPGNRHLRKGDLVILDYGAKWKGYCSDITRTIALGKPNERLVEIYETVKEAQEKAYRTVREGVKAKDVDAAARGVISAAGYGKYFTHRTGHGLGLDVHEEPYIGPDSEVILENGMTFTIEPGIYVPGLGGVRIEDDVAVIDGRGKRLTRAERELITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7A4</accession>
    <name>A0A218P7A4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CRISPR-associated endoribonuclease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_04675</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the CRISPR-associated protein Cas6/Cse3/CasE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853922.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7A4"/>
    <dbReference type="GeneID" id="33315539"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04675"/>
    <dbReference type="OrthoDB" id="43942at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09759">
        <property type="entry name" value="Cas6_I-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049435">
        <property type="entry name" value="Cas_Cas6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010156">
        <property type="entry name" value="CRISPR-assoc_prot_Cas6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045747">
        <property type="entry name" value="CRISPR-assoc_prot_Cas6_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01877">
        <property type="entry name" value="cas_cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36984">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS6 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36984:SF1">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS6 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21350">
        <property type="entry name" value="Cas6_I-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01881">
        <property type="entry name" value="Cas_Cas6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005054">
        <property type="entry name" value="PF1131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CRISPR associated protein Cas6 C-terminal" evidence="7">
        <location>
            <begin position="120"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="29"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="5">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR005054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005054-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR005054-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="262" mass="30342" checksum="35311714AC8DBCED" modified="2017-09-27" version="1">MRFLIRLLPENEPFKIPFNHQHYLQGLIYRRIQRLNPELSLSLHRPKVPKLFTYSLFMAEKRELAGDRSGLLGHGHGFFYFSTAVPEIAEAFIGGLLENPEVELWGERFTVREVKALYEPEKLSGRKFVTLSPVAVTTRKVQFGRPKSYDLSPAEPEFYELIRENLREKYVHLHGERPPEEFEMKVLSAKPKRFEVKPGIFQTAWHLVFRAKGDEGLLRAGYLAGFGEKNSIGFGMVKVDGRKPKVKKKWRGGERNRESKEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7E6</accession>
    <name>A0A218P7E6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_04915</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853959.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7E6"/>
    <dbReference type="GeneID" id="33315587"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04915"/>
    <dbReference type="OrthoDB" id="7531at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052920">
        <property type="entry name" value="DNA-binding_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000383">
        <property type="entry name" value="Xaa-Pro-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43358">
        <property type="entry name" value="ALPHA/BETA-HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43358:SF4">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02129">
        <property type="entry name" value="Peptidase_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xaa-Pro dipeptidyl-peptidase-like" evidence="1">
        <location>
            <begin position="80"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="288" mass="32949" checksum="CE335B8692C739AE" modified="2017-09-27" version="1">MIIEVLLLVIFLFLAFSAFVGYKMVKPPRLIGDWTPKDLGFDYEDITFETEDGLKLSGWWIPNGSDKTVIPLHGYTASRWYELYMKPTVEFLLKEGYNVLVFDFRAHGKSEGKYTTVGDKEIADVRAAVKWLKENHPEEARRIALVGFSMGAMLTIRSLAEIEEVCCGVADSPPMDLDKTGARGLKYFAGLPEWLHVFVKPFTKLFSGGKEVNPIEYADKVKKPLLLIAGEKDPLVTVEEVREFYERNKAINPDVELWVTDAPHVRTLKLHPEEWKEKVLNFLTRHLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7H5</accession>
    <name>A0A218P7H5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MazF family transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05075</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853985.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7H5"/>
    <dbReference type="GeneID" id="33315619"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05075"/>
    <dbReference type="OrthoDB" id="90963at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003477">
        <property type="entry name" value="PemK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011067">
        <property type="entry name" value="Plasmid_toxin/cell-grow_inhib"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02452">
        <property type="entry name" value="PemK_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50118">
        <property type="entry name" value="Cell growth inhibitor/plasmid maintenance toxic component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="12397" checksum="9498D15C45F05C4B" modified="2017-09-27" version="1">MKGKIVLVPFPFTSLRGAKLRPALVLFEDKDDVTLAFISSRVERYNPETDVLIEKSHPEFHLTGLKVSSFVRLTKIATVQKDILIGELGEIGPILKKEISQKICSLLGFES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7K4</accession>
    <name>A0A218P7K4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_05215</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854008.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7K4"/>
    <dbReference type="GeneID" id="33315647"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05215"/>
    <dbReference type="OrthoDB" id="86163at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="121"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="94"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="319" mass="37341" checksum="209AA79CF551AEF6" modified="2017-09-27" version="1">MEEILKAIEERNCKKVAELLYHKVDELGDEELKEVLEKAKKLALECEDFELYKLTIYYFHELLGIDMVNEFEKLAEEKDTFEAKFELADLYYLLGELEKSLDLYRALLEEETAKGNVENVAKVYYSMALIHEELQEYEKALELMDRAEEIYRDLGDEEQLLRVGIHRAYVLFESGEVYEAKAMLAGLLPKVLDKKDLLVELHLSFEEIFEDDENYDAALQECLYALIHAKGTDYEDVAFGSLMDVLWQLFLEDDFETVYLNIDMFARAFPDMKDFFEAVKAIALYKDGKMSEEEVSKYIEKVTDQRQIDLLEFLGEAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7L1</accession>
    <name>A0A218P7L1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hydrogenase maturation factor HypA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">hypA</name>
        <name evidence="6" type="ORF">A3L08_05300</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the maturation of [NiFe] hydrogenases. Required for nickel insertion into the metal center of the hydrogenase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the HypA/HybF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854024.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7L1"/>
    <dbReference type="GeneID" id="33315664"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05300"/>
    <dbReference type="OrthoDB" id="36835at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2320.80:FF:000004">
        <property type="entry name" value="Hydrogenase maturation factor HypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00213">
        <property type="entry name" value="HypA_HybF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020538">
        <property type="entry name" value="Hydgase_Ni_incorp_HypA/HybF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000688">
        <property type="entry name" value="HypA/HybF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003008">
        <property type="entry name" value="PRK03824.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34535">
        <property type="entry name" value="HYDROGENASE MATURATION FACTOR HYPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34535:SF3">
        <property type="entry name" value="HYDROGENASE MATURATION FACTOR HYPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01155">
        <property type="entry name" value="HypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004761">
        <property type="entry name" value="Hydrgn_mat_HypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01249">
        <property type="entry name" value="HYPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0533">Nickel</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="2"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="139" mass="15711" checksum="9E45D2D1E9A46B7F" modified="2017-09-27" version="1">MHEWALADAIVRTVLDYANREGAERVKAVKVVLGELQDVAEDIVKFAMEQLFAGTIAEGAEIIFEEEEAVFRCRNCGHEWKLKEVKDKFDERIKEDIHFIPEVVHAFMACPKCGSHDFEVVQGRGVYVAGIMIEKGGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7M7</accession>
    <name>A0A218P7M7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854029.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7M7"/>
    <dbReference type="GeneID" id="33315669"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05325"/>
    <dbReference type="OrthoDB" id="90142at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="76" mass="8895" checksum="C03F9DE13F4C03B1" modified="2017-09-27" version="1">MIEYVWLVAGILGVVSALLDLKAEESREETLKDLFLGTGFLLWYFRRDVLGSIFILAAVLVYLPELRKKWIRWRHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Q5</accession>
    <name>A0A218P7Q5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Threonine--tRNA ligase</fullName>
            <ecNumber evidence="14">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Threonyl-tRNA synthetase</fullName>
            <shortName evidence="14">ThrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">thrS</name>
        <name evidence="16" type="ORF">A3L08_05575</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the class-II aminoacyl-tRNA synthetase family. Archaea-specific ThrRS editing domain subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="14"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854075.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Q5"/>
    <dbReference type="GeneID" id="33315719"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05575"/>
    <dbReference type="OrthoDB" id="372136at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00860">
        <property type="entry name" value="ThrRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000076">
        <property type="entry name" value="Threonine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000001">
        <property type="entry name" value="Threonine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.80.10:FF:000004">
        <property type="entry name" value="Threonine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.80.10">
        <property type="entry name" value="D-tyrosyl-tRNA(Tyr) deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023509">
        <property type="entry name" value="DTD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015011">
        <property type="entry name" value="Threonyl-tRNA_syn_edit_dom_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047246">
        <property type="entry name" value="ThrRS_anticodon"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003068">
        <property type="entry name" value="PRK03991.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF44">
        <property type="entry name" value="THREONINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08915">
        <property type="entry name" value="tRNA-Thr_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 14 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 14" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 14" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="7 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="15">
        <location>
            <begin position="231"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="475"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ASJ06825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="626" mass="72943" checksum="16236C0A04E2D80B" modified="2017-09-27" version="1">MRMLLIHSDYLEYEVKDKALKNPEPISDEQRKGRLDEVLAVFMSVEKVDESNPDEVVEKAVKEIKDVASQVKAEKIFVYPFAHLSSELAKPDVALKILQKIEEKLKEEGFEVKRAPFGYYKAFKLSCKGHPLAELSRTIVPSGEAVSKEERNIALEKEEELKSYWYVLTPEGELIEVDKFDFTGHENLRKFANYEISKNRVADREPPHVKLMLEHELVDYEPGSDGGNLRYYPKGRLIKGLLEQYVTEKVVEYGAMEVETPIMYDFEHPALEKYLNRFPARQYVVKSGDKKFFLRFAACFGQFLIKKDATISYRNLPLRMYELTRYSFRREKSGELSGLRRLRAFTMPDMHTVARDLKQAMDEFKKQYKLSMEVLRGVGLTPDDYEVAIRFTEDFWNGNRDFIVELAKIIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDEEGQEQYPLILHCSPSGAIERVMYAILEKQAKLQAKGQKPMFPLWLSPIQVRVIPVSDDVMDYALYVAGKLEGAKIRVDVDDTNDRLNKKIRKAEKEWVPYIIVVGNNEKEQNTVTVRRRSDGKQVEMQLEDLIREIKAQTEGFPYRPRPLPLLLSRRPKFRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7R7</accession>
    <name>A0A218P7R7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Inosine-5-monophosphate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_05500</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854063.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7R7"/>
    <dbReference type="GeneID" id="33315704"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05500"/>
    <dbReference type="OrthoDB" id="9280at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02205">
        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014651">
        <property type="entry name" value="UCP036983_2CBS_MJ1404"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036983">
        <property type="entry name" value="UCP_2CBS_MJ1404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="72"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="136"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="214"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="391" mass="44446" checksum="E40D661DD92A7D2F" modified="2017-09-27" version="1">MVGILVQEVMTDRFQKIDIDAPLSEAIGIFEKEDPDLILVFDGNLYKGVLTQDLIIRSHLKWDPTKAKVRDVYKPAPVIKPDEDLSKAAKLMMEVDLRSLPVGESKAEIIGVISDIELLKRVSEGDFGKRKIEEFMTKDVITLKPDDTVAKAFATMRDHAISRIPIVNEAGKLEGLVTLHDLIIRFIKPRFKAQAGEVAGEKIPPFSMKLRDVMIRGVITILPDAKVREAVATMKDNDIDGLVVVNEDNRVVGILTVKDLLLPISRMVEKEARFYLQLGGDAEILSDFTRERIIEDVRRFVDGYEDLLGQEGIIYLYIRRFPEKFRGVHLYQARMRVVTDRGVFVATGETWGAIQAVHDALRAIERQLLQKAELEKDTHYYKRFIEKMGLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7T2</accession>
    <name>A0A218P7T2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">AI-2E family transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_05620</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854084.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7T2"/>
    <dbReference type="GeneID" id="33315728"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05620"/>
    <dbReference type="OrthoDB" id="137390at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716:SF71">
        <property type="entry name" value="TRANSPORT PROTEIN MJ1177-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="5"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="215"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="287"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="336" mass="37070" checksum="C4151FF44E9DA993" modified="2017-09-27" version="1">MEIEAAVWVAISLVVLYLVWETVSPILSPLIIAATLAYILYPFHERLSKRFGNRLSALTMTGVLTILTLLFIIGYALWINDVKHSLAHYIEVFFQWLLGFHLPQAMYELVQRLAEDIPRRLEEYVLGYTYSLPKLTLQAIVMVFTFYGLLVNTRAIREEVYALLPMENRELAAKLLGSAGRTLHNLLRGWLAVSVLKGSAIALGFYLFSVSSAGGAIAAGIFTMVFELLPVLGGWMIWLAGAVYLFNSGSLLAALMLSAYGFVLVSPMPDYFLRSKLGKRETGVNSLISLVGIFGGYIAFGFVGIIIGPVALSLLGTLLDEWKKTKEARSRAGTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P854</accession>
    <name>A0A218P854_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Archaeal Lon protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">A3L08_06440</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">P-4</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="13">Homohexamer. Organized in a ring with a central cavity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S16 family. Archaeal LonB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854235.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P854"/>
    <dbReference type="GeneID" id="33315891"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06440"/>
    <dbReference type="OrthoDB" id="64652at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004663">
        <property type="entry name" value="Lon_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046843">
        <property type="entry name" value="LonB_AAA-LID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00764">
        <property type="entry name" value="lon_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="ATP DEPENDENT LON PROTEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20436">
        <property type="entry name" value="LonB_AAA-LID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01078">
        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 14 18" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="16">
        <location>
            <begin position="167"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="17">
        <location>
            <begin position="436"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="522"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00026070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ASJ06986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="634" mass="69849" checksum="5F671E9C208C8F80" modified="2017-09-27" version="1">MGDEEKVGEALAPKEYGESLELGVEFSTTEEIKVPEKLIDQVIGQEHAVEVIKTAANQKRHVLLIGEPGTGKSMLGQAMAELLPTENLEDVLVFPNPEDENMPKIKTVPACQGRRIVERYREKAKGQENIKSYILLFVMFMVMMAIFMEPSATTILLGIFVVIMTMVALSNMRFRNSVLIPKLLIDNCGRTKAPFIDATGAHAGALLGDVRHDPFQSGGLGTPAHERVEPGMIHRAHKGVLFIDEIATLSLKMQQSLLTAMQEKKFPITGQSEMSSGAMVRTEPVPCDFILVAAGNLDTIDKMHPALRSRIRGYGYEVYMRTTMPDTLENRKKLVQFVAQEVVRDGKIPHFTRDAVEEIVREAQKRAGRKGHLTLRLRDLGGIVRAAGDIAVKKGKKYVEREDVLEAIRMAKPLEKQLADWYIENKKEYQVIRTEGSQVGRVNGLAVIGEQSGIVLPIEAVVAPAASKEEGKIIVTGKLGEIAKEAVQNVSAIIKRYKGEDISRYDIHVQFLQTYEGVEGDSASISVATAVISALEGIPIRQDVAMTGSLSVLGEVLPIGGATPKIEAAIEAGIKTVIIPKANEKDVFLSKDKAERIEIFPVETIDQVLEIALEDNEKKRELLKRIREALPLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P893</accession>
    <name>A0A218P893_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 14">S-adenosylmethionine synthase</fullName>
            <shortName evidence="14">AdoMet synthase</shortName>
            <ecNumber evidence="5 14">2.5.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 14">Methionine adenosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">mat</name>
        <name evidence="15" type="ORF">A3L08_06665</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 14">Catalyzes the formation of S-adenosylmethionine from methionine and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>L-methionine + ATP + H2O = S-adenosyl-L-methionine + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the AdoMet synthase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="5 14"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854276.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P893"/>
    <dbReference type="GeneID" id="33315936"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06665"/>
    <dbReference type="OrthoDB" id="204488at2157"/>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.280">
        <property type="entry name" value="S-adenosylmethionine synthetase, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00136">
        <property type="entry name" value="S_AdoMet_synth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027790">
        <property type="entry name" value="AdoMet_synthase_2_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042544">
        <property type="entry name" value="AdoMet_synthase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002795">
        <property type="entry name" value="S-AdoMet_synthetase_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003364">
        <property type="entry name" value="PRK04439.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003366">
        <property type="entry name" value="PRK04439.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36697">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36697:SF1">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01941">
        <property type="entry name" value="AdoMet_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 14" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="139"/>
            <end position="144"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ASJ07026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="405" mass="44929" checksum="A074DC9C0F7E4EA1" modified="2017-09-27" version="1">MAEKVRNIVVEEMVRTPVEMQKVELVERKGIGHPDSIADGIAEAVSRALSREYIKRYGIILHHNTDQVEVVGGRAYPRFGGGEVIKPIYILLSGRAVEMVDREFFPVHEIAIKAAREYLKKAVRHLDLEHHVIIDSRIGQGSVDLVGVFNKAKENPIPLANDTSFGVGYAPLSETERIVYETEKLLNSDEFKKKWPAVGEDIKVMGLRKGDEIDLTIAAAIVDSEVDNPDDYMAVKEAIYEAAKEVVESHTQRKVNIHVNTADDPKKDIYYITVTGTSAEAGDDGSVGRGNRVNGLITPNRHMSMEAAAGKNPVSHVGKIYNLLSMLIANDIAEQVEGVEEVYVRILSQIGKPIDEPLVASVQIIPKKGYSLDVIERPAYEIADAWLADINKIQKMILEDKLNVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P899</accession>
    <name>A0A218P899_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sulfhydrogenase 1 subunit delta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_06485</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854243.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P899"/>
    <dbReference type="GeneID" id="33315900"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06485"/>
    <dbReference type="OrthoDB" id="37913at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.700">
        <property type="entry name" value="NADH:ubiquinone oxidoreductase-like, 20kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051349">
        <property type="entry name" value="Hydrogenase_assoc-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053625">
        <property type="entry name" value="NADPH-dep_hydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037024">
        <property type="entry name" value="NiFe_Hase_small_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040836">
        <property type="entry name" value="sulfhyd_HydD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42845">
        <property type="entry name" value="COENZYME F420-REDUCING HYDROGENASE, GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42845:SF3">
        <property type="entry name" value="CYTOSOLIC NIFE-HYDROGENASE, DELTA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56770">
        <property type="entry name" value="HydA/Nqo6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase-like 20kDa subunit" evidence="2">
        <location>
            <begin position="17"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="265" mass="29794" checksum="4D3A83C4B286ADE4" modified="2017-09-27" version="1">MEENGKVRIGFYALTSCYGCQLQFAMMDEILQLLPNAEIVCWYMLERDSKEIEPVDIAFIEGSVSTEEEVELVKKIRENAKTVVAVGSCAVHGGVQSWEKDKSLDELWKTVYGDGTVKFQPKMAEPVEKYIKVDYKIYGCPPEKKDFVYAIGTLLIGSWPEDIDYPVCLECRLNGNSCVLLEKGEPCLGPVTRAGCNARCPAYGIACIGCRGAIGYDVAWFDSLARVFREKGLTKEEILERMKIFNAHNPKLEEMVNKIFEGVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8E4</accession>
    <name>A0A218P8E4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06885</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854316.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8E4"/>
    <dbReference type="GeneID" id="33315980"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06885"/>
    <dbReference type="OrthoDB" id="97428at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="375" mass="42793" checksum="27B11E7E778E751E" modified="2017-09-27" version="1">MEVNWKFGTDRETLLNNLRELSATGDVEYYRSYVLEDRDYLVIARDVPIFSADCGILRAIEELAAFNFSILGVRRAIRPVKLRTGRVEASPYLDALTDGNLRPFLEEHPVLSLGSYDPFIDLHLVLDGERIHVLSLEWYGPHQWIYDVIPVDEWLDGTVRFIAMAVRDMEAMRETLLRFGYSNYPRALGRAKALLRLLLDAYPVDIDSLPPAYAPWEVEEISRLASMLLLKGNVAGALEVISTLKNPNHFLTAVFEIGSDVPFNVVEEFYRRLPAGHRERALAHLVYRITWGGLYGSGIKLARELGDDLAYHSAAVALIDSGLHEAALKMAEKIENRWRKGEVLLKVYFERPDLAEEIKKMAPRHVRTFIDWRGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8H4</accession>
    <name>A0A218P8H4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Damage-control phosphatase ARMT1-like metal-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854345.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8H4"/>
    <dbReference type="GeneID" id="33316014"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07055"/>
    <dbReference type="OrthoDB" id="359165at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.380">
        <property type="entry name" value="Uncharacterised protein PF01937, DUF89, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.285.20">
        <property type="entry name" value="Uncharacterised protein PF01937, DUF89, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10880">
        <property type="entry name" value="Uncharacterised protein PF01937, DUF89, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036075">
        <property type="entry name" value="ARMT-1-like_metal-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002791">
        <property type="entry name" value="ARMT1-like_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053636">
        <property type="entry name" value="DCN_phosphatase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014444">
        <property type="entry name" value="PH1575-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040824">
        <property type="entry name" value="damage_ctl_Phtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01937">
        <property type="entry name" value="ARMT1-like_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006593">
        <property type="entry name" value="UCP006593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111321">
        <property type="entry name" value="AF1104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Damage-control phosphatase ARMT1-like metal-binding" evidence="1">
        <location>
            <begin position="4"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="282" mass="31434" checksum="80B81E5F843389F6" modified="2017-09-27" version="1">MKVHYECFACAANQCQKIVEIGTNDLELRRRGVIEAAKLMAGINGDSIPAVFGSEVFLGVYRVIGNDDPFKEYKELSNRLAEKVVSDIEKEGPNLKTALKLAIIGNVIDFAVGYSPERIEEDVKAMLSEELYVDHSDELFERLGKAKTLLYLADNCGEIYFDRLFIKKLKEAFPYLKIYVAAKDGPIINDATVEDLRKACFGEFAGIISTGTRIVGVPLDRVSDEFREVFEKADVIIAKGQGNFETLSEVKDNRVFYLLKAKCRPIARELGVPQGSMVCLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8J9</accession>
    <name>A0A218P8J9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">IFT52 GIFT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_07125</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854359.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8J9"/>
    <dbReference type="GeneID" id="33316028"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07125"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039975">
        <property type="entry name" value="IFT52"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055458">
        <property type="entry name" value="IFT52_GIFT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12969:SF7">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 52 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12969">
        <property type="entry name" value="NGD5/OSM-6/IFT52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23355">
        <property type="entry name" value="IFT52_GIFT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IFT52 GIFT" evidence="2">
        <location>
            <begin position="500"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="443"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="445"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="719" mass="80881" checksum="B3D25AB8EFE66C2A" modified="2017-09-27" version="1">MRKSAMAITLILILSLIPTWAVKPVKANDATYVPLIELNQNFDEYAYNGDILTRGIVTYVDNSGFAIQNGTGPYTGIYVYTGYNSHPAVTPGDLVEVYGFPKYYDGLRELSVNPRYGEYYNVVGSAEVPEPVVIPTADYDKSEYQSVLVKFVDAKIIDRYDRGYTKLWIDDGSGEAYVFSYSSLPSDLQPGAKFKYLTGLVYVYRNSYEVIPLSWELYNPAVKITNVEYYAIIQGVSSPVWVTVKNEGDKDTTVDLKVLLDGVLLHDEQFELGLGKSKEITVYVLSNDSGMHTLSIFAEGSSIELKTWVIPAPHSIAYGLTYYYERRYNEFLNQILPLYENMTFILQEFDKCGVNLGDIEPEIQIVHKLKEEMDLEHQIYTKLNQKTSLKNPYLGSSYYFAVMIHARKASLSAEKLLNELNFVVPVLWKNYNVIMPICNPPAEEQPSNETVPSNESEVTPGNETIPSNQTNMSPSMNITITIPKVLIDASHGQYYNPTKTDQSGLSTLINNIKNELGWIVDVNTETITYEKLQEYDVLIITNPTDDISDEEAQAIQEFVENGGGLFILGDWYKYANTENLNAIVGKYGIKFNADELMDDEQNSGRPYYPFVGIYNKDHPAMKFVPDDWTMYYNGQTLTIGGSAVWLIKAYDTGYSVDADGNVVFEKGSEPVIAAAVEVGTGRIIAYGSSKALSDSYYNKYIDSNWPFIKGALLWLAHQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8K1</accession>
    <name>A0A218P8K1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF763 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_07065</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854347.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8K1"/>
    <dbReference type="GeneID" id="33316016"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07065"/>
    <dbReference type="OrthoDB" id="9948at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008482">
        <property type="entry name" value="DUF763"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38597">
        <property type="entry name" value="BLL3834 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38597:SF1">
        <property type="entry name" value="BLL3834 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05559">
        <property type="entry name" value="DUF763"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="381" mass="43870" checksum="E1E834A794FC496F" modified="2017-09-27" version="1">MRNVADLPLHGGHVPGWLAQRMRKLTRLVLILAVEEYGTRGLLKRLFDPVWFQAFNNVIGMDWDSSGSTTVTAGMIKDALWREELGVKAAGGKGKKSRATPEELKTIAELYELDPEPYVRTSRLVAKVDTVALQTGYQLYHHVFFLDEEGNWAVVQQGMNERERLARRFHWFDTETFTLDPHNAISGLKREFALNTVSREAREYQKTLLDVIQEKPAKIERELEGLKAIAKGYRPLVYYKPREPWEKDILKRYESLGKFELNKRALEFARELSVSNYEEFLLLKGLGPSTLRALSLVLELVYDVHPSWKDPVTHPPDPFKFSYAVGGKDKVPFPIDKPAYDELISFLEELVSRHPEEKALVRNVTKITKNWEFPEGEKRAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8M9</accession>
    <name>A0A218P8M9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">A3L08_07325</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854397.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8M9"/>
    <dbReference type="GeneID" id="33316068"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07325"/>
    <dbReference type="OrthoDB" id="31344at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05145">
        <property type="entry name" value="RIO1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051272">
        <property type="entry name" value="RIO-type_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018935">
        <property type="entry name" value="RIO_kinase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45723">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01245">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="52"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ07145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="257" mass="30594" checksum="6F533FCA92C24A9C" modified="2017-09-27" version="1">MREDFIEREIEDMLGLRERREKDSELYKIANEVFDRSTKETLAYLHRRGKIEALHGVISTGKEANVFAGVDSDGNRIAVKIYRTYTTEFRRIWEYLAADPRIGYLPKDMRKLVFVWTRREFKNLQRAIKYAVRVPEPITFRNNVLVMEFIGDELPAPRLKDVERELQREDFEELYTYLMGVIERLWKRGDMVHGDLSEYNVLLWDSPVVIDWSQATVKRNRMSVELLKRDLRNIINYFGRKGVDVDDFDDKFRELAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Q8</accession>
    <name>A0A218P8Q8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase</fullName>
            <ecNumber evidence="9">2.5.1.114</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">tRNA wyosine derivatives biosynthesis protein Taw2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">taw2</name>
        <name evidence="11" type="ORF">A3L08_07405</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">S-adenosyl-L-methionine-dependent transferase that acts as a component of the wyosine derivatives biosynthesis pathway. Catalyzes the transfer of the alpha-amino-alpha-carboxypropyl (acp) group from S-adenosyl-L-methionine to 4-demethylwyosine (imG-14), forming 7-aminocarboxypropyl-demethylwyosine (wybutosine-86) at position 37 of tRNA(Phe).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 9">
            <text>4-demethylwyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36355"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10378"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73550"/>
            <dbReference type="EC" id="2.5.1.114"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>guanosine(37) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36899"/>
            <dbReference type="Rhea" id="RHEA-COMP:10145"/>
            <dbReference type="Rhea" id="RHEA-COMP:10147"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.228"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.114" evidence="9"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854410.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Q8"/>
    <dbReference type="GeneID" id="33316084"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07405"/>
    <dbReference type="OrthoDB" id="8079at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052906">
        <property type="term" value="F:tRNA (guanine(37)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102522">
        <property type="term" value="F:tRNA 4-demethylwyosine alpha-amino-alpha-carboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.110:FF:000001">
        <property type="entry name" value="tRNA (guanine(37)-N1)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000431">
        <property type="entry name" value="tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.110">
        <property type="entry name" value="Met-10+ protein-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01922">
        <property type="entry name" value="TYW2_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056744">
        <property type="entry name" value="TRM5/TYW2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030867">
        <property type="entry name" value="TYW2_archaea"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047823">
        <property type="entry name" value="tRNAwyosTaw2Pyroc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF41">
        <property type="entry name" value="TRNA(PHE) (4-DEMETHYLWYOSINE(37)-C(7)) AMINOCARBOXYPROPYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25133">
        <property type="entry name" value="TYW2_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="10">
        <location>
            <begin position="34"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="187"/>
            <end position="188"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00058518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ07159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="282" mass="32419" checksum="65CF5C33D57A2600" modified="2017-09-27" version="1">MSGRRKTQLIKPRIREILSRELPEGLVELLPKHWVQIGDVLILPLRPELEPYKGRIAEVYAQVIGVKTVLRKGRISGEFRETNYEVLYGGDTVTVHVENRIKYKLDVARIMFSPANVKERVRMAKVAKPGELVVDMFAGIGHLSLPMAVHGGARVIAIEKSPYTFQFLVENIELNRVQDRMTAYNMDNRDFPGENIADRILMGYVVTTHEFIPKALQIAKDEAVIHYHNTVPERLMPAEPFETFRRIAREHGYEAEKLNELIIKRYAPGVWHVVVDVRVFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Q9</accession>
    <name>A0A218P8Q9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_07480</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854423.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Q9"/>
    <dbReference type="GeneID" id="33316099"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07480"/>
    <dbReference type="OrthoDB" id="101068at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="78" mass="9030" checksum="3388E4C6DB23B942" modified="2017-09-27" version="1">MKAHKRYEVYLKSTYDGIFGRSVRGVELSLILLEDHARFSGVLSFLLPYSEKNARELLELLKVLVEKAKLYAEAENLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8S8</accession>
    <name>A0A218P8S8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">tRNA (Guanine-N2)-dimethyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_07670</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854455.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8S8"/>
    <dbReference type="GeneID" id="33316137"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07670"/>
    <dbReference type="OrthoDB" id="14987at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18099">
        <property type="entry name" value="Trm10arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028564">
        <property type="entry name" value="MT_TRM10-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038459">
        <property type="entry name" value="MT_TRM10-typ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016742">
        <property type="entry name" value="tRNA_m1G_mtfrase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053618">
        <property type="entry name" value="tRNA_N1-methyltransferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041140">
        <property type="entry name" value="Trm10AGmtase_Thcocales"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018978">
        <property type="entry name" value="tRNA_m1G_mtfrase_arc_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51675">
        <property type="entry name" value="SAM_MT_TRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase TRM10-type" evidence="4">
        <location>
            <begin position="87"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="369" mass="43056" checksum="32F9BCEE0AA33E6F" modified="2017-09-27" version="1">MKTLADVFREVLMEKGIESIGTLSKRFRKSKNKLQDIAIEIVHGKGAIFRVPEKTAVAWDLSGKRVEGSYYAYAPLCMMEKFEPILTPDELRLRLPDWPYFIVDLQLWDKHTQKEKGKVCLQINQCYGLLRDYFTGSEVAVTWANEEFRKMFHGPLDRITVYDDPTAEFLKENKIDEVVLLDPWADEVLSEKDFDVKAFIIGGIVDTGGEKKLTPKIGEELEKAGIKVRRRKIVLKGDIIGVPDRINRILGIILKMMVEGKSMDEAVYEMQEPLHARWRLRKELPKRAIRYKVDGKTYRVVEKELFDYYSSWLKIRWEDFVKVLRELDLIALERKRIHHLNKISNARIINGKAYRVLLLKKAAMLCYNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8U6</accession>
    <name>A0A218P8U6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_07470</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854421.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8U6"/>
    <dbReference type="GeneID" id="33316097"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07470"/>
    <dbReference type="OrthoDB" id="30658at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03236">
        <property type="entry name" value="ABC_RNaseL_inhibitor_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000152">
        <property type="entry name" value="ATP-binding cassette, sub-family E, member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001546">
        <property type="entry name" value="RNase L inhibitor homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013283">
        <property type="entry name" value="RLI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034348">
        <property type="entry name" value="RLI_dom_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009945">
        <property type="entry name" value="PRK13409.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19248">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="Fer4_RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01868">
        <property type="entry name" value="ABCEFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="3"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="43"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="67"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="334"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="559"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="592" mass="66644" checksum="29B4046030042AFF" modified="2017-09-27" version="1">MSRIAVIDYDKCNPDKCGHFLCERVCPVNRMGGEAIIIDEENYKPIIQEASCTGCGICVHKCPFNAITIVNLPEQLDEDCVHRYGINSFVLYRLPIVKDGMVVGILGPNGTGKTTAVKILSGQLLPNLCGDNDSWDNVIKAFRGNELQNYFESLKNREIRPVVKPQYVDLIPKAVKGKVRDLLKKADEAGKFDEVVRELELENVLDRDIKHLSGGELQRVAIAAAILRDAHFYFFDEPSSYLDIRQRLKVARIIRHLADSGKAVLTVEHDLAVLDYLSDIIHVVYGKPGAYGIFSQPKSTRNGINEFLRGYLRDENVRFRPYEIRFTKSSERTSQASDILVEYPRLVKDYGGFRLEAEPGTLYMGEVVSIVGPNGIGKTTFVKMLAGVEEPTEGEIDWELKVSYKPQYIKADYEGTVFDLLSKIDAAKLMNSFYKTELLNPLGIPDLYDKQVNELSGGELQRVAITAALIREADLYLLDEPSAYLDVEQRLAVSRAIRHLMEKEGKTALVVEHDVLMIDYISDRLMVFEGEPGRQGKALPPMGMREGMNRFLASVGITFRRDPDTGRPRANKEGSVKDREQKEVGEYYYVAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Y3</accession>
    <name>A0A218P8Y3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription factor S</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Transcription elongation factor IIS/RNA polymerase subunit homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">A3L08_07940</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="9 13">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854503.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Y3"/>
    <dbReference type="GeneID" id="58918975"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07940"/>
    <dbReference type="OrthoDB" id="72957at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10511">
        <property type="entry name" value="Zn-ribbon_TFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000029">
        <property type="entry name" value="DNA-directed RNA polymerase subunit M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019761">
        <property type="entry name" value="DNA-dir_RNA_pol-M_15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006288">
        <property type="entry name" value="TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01384">
        <property type="entry name" value="TFS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF12">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01030">
        <property type="entry name" value="RNA_POL_M_15KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00466">
        <property type="entry name" value="ZF_TFIIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 11 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="14">
        <location>
            <begin position="67"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="11">
        <location>
            <begin position="4"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="4"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ASJ07255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="110" mass="12926" checksum="0BE56D754020F648" modified="2017-09-27" version="1">MKFCPKCGNLMLPDRKRKVWVCRSCGYEEPFDEEKDREKTKITQKVEHKPDEGIIVVEQDVKTLPTTKVTCPKCGNDTAYWWEMQTRAGDEPSTIFYKCTKCGHVWRAYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Z7</accession>
    <name>A0A218P8Z7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_08035</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
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    <dbReference type="GeneID" id="33316212"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08035"/>
    <dbReference type="OrthoDB" id="132045at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
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    </dbReference>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004256">
        <property type="entry name" value="DUF234"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34704">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34704:SF2">
        <property type="entry name" value="ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03008">
        <property type="entry name" value="DUF234"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase" evidence="2">
        <location>
            <begin position="5"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="240"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="DUF234" evidence="3">
        <location>
            <begin position="307"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="455" mass="54399" checksum="9CAABEDB94224981" modified="2017-09-27" version="1">MILKFIDREPELKTLEGLYAQDRAQLVLIYGRRRIGKTELVKQFIKGRQSFYFLARKEPMELELDRLLRSFNRKFNVFIEARNLEEFFEEVRKFGKLIFVIDEFPYWVEKNKSIPSTFQYIWDEILMDSKIMLILMGSSISTMESLMSYKNPLYGRRTAQIKLSTLGFFHLREAFPRYSWEDLVKVYGTIDGIPAYLQYFDDSMSVEENIERNFYSRVSILYEDAERLLKDELREPVTYLNILRAINEGKTKLTEIASETKVAVTNLPKYLKTLETLDLVYKEFPVTVRQRRRFGIYRVKDFYYRFWLRFVYPYRDDIEIGAISFDDLRKDFNVYLGEVFERVAREFLMALNAKGKLPFRFTKIGRWWDKGEEIDLVAVNSLTNEAGFFEAKWKELSRREALRVLRRLEEKSEMVNIKGEKRYHGLIARHVEDKEGLREEGYLVFDIGDFHRTIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P906</accession>
    <name>A0A218P906_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07945</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854504.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P906"/>
    <dbReference type="GeneID" id="33316194"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07945"/>
    <dbReference type="OrthoDB" id="101908at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="62" mass="7352" checksum="95FD6333D7452649" modified="2017-09-27" version="1">MTRTNSRELTELEKETLRKLAEKALKELEEAYRRIPEADNGKAYLFRGKERVRLMLDILKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P932</accession>
    <name>A0A218P932_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ABC transmembrane type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_08195</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P932"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08195"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051393">
        <property type="entry name" value="ABC_transporter_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30193">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30193:SF37">
        <property type="entry name" value="INNER MEMBRANE ABC TRANSPORTER PERMEASE PROTEIN YCJO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="258"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="64"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="286" mass="32807" checksum="4B438DFFC469203C" modified="2017-09-27" version="1">MKYTLEAYGFLAPALVILIMFIIYPILNLFYISFFDWRGLGAKEFVFLNNYRMILQREEFRQVLKNTFVYILGVVPTTMVLSLIAAVLLNTKIRLKSLYRTSIFSPTAISFVATGIIWVWMFNSDYGLVNRVLEALGFSTLDWLKSTSYAMLAVIIATVWARVGYFMVIYLAGLQTIPEEYYEAAEVDGATSFRKFLHITLPLLKPTHVLVLVMLTIFSFRDFDQIYTMTGGGPLMSTTTLAYYIYVLSFERFRFSEGATVAVILLFLVIAVQYLQRKAFGEEAIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P951</accession>
    <name>A0A218P951_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Superoxide reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_08250</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the desulfoferrodoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854558.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P951"/>
    <dbReference type="GeneID" id="33316256"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08250"/>
    <dbReference type="OrthoDB" id="30725at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03172">
        <property type="entry name" value="SORL_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.730">
        <property type="entry name" value="SOR catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002742">
        <property type="entry name" value="Desulfoferrodoxin_Fe-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036073">
        <property type="entry name" value="Desulfoferrodoxin_Fe-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051233">
        <property type="entry name" value="Desulfoferrodoxin_SOR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00332">
        <property type="entry name" value="neela_ferrous"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36541">
        <property type="entry name" value="SUPEROXIDE REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36541:SF1">
        <property type="entry name" value="SUPEROXIDE REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01880">
        <property type="entry name" value="Desulfoferrodox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49367">
        <property type="entry name" value="Superoxide reductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Desulfoferrodoxin ferrous iron-binding" evidence="6">
        <location>
            <begin position="9"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="115" mass="13189" checksum="8A606F4CBFDDE2EC" modified="2017-09-27" version="1">MLSGTIKSGDWKGEKHVPVIEYEKEGNLVKVEVSVGKEIPHPNTPEHHIAWIELYFHPEGENFPIMVGRVAFTNHSDPLTEPRAAFFFRTEKKGKLYALSYCNIHGLWENEVTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9D8</accession>
    <name>A0A218P9D8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lysophospholipase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9D8"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08455"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000117">
        <property type="entry name" value="Monoglyceride lipase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051044">
        <property type="entry name" value="MAG_DAG_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11614">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="14"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="261" mass="28768" checksum="F292E319B99BF678" modified="2017-09-27" version="1">MVVEVYKAKFGTPERGWVVLVHGLGEHSGRYGKLIGMLNEAGFAVYTFDWPGHGKSPGKRGHTSVEEAMEIIDSIIAELGENPFLFGHSLGGLTVVRYAETRPDKIRGVVASSPALAKSPETPGFMVALAKFLGRVAPGLTLSNGIKPELLSRNPEAVKAYVEDPLVHDRISSKLGRSIFENMEKAHREAERIKVPVLLLVGTGDVITPPEGSRRLFEELKVEDKELKEFPGAYHEIFEDPEWGKALHKKIIEWLLEHSGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9E0</accession>
    <name>A0A218P9E0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glyoxylate reductase</fullName>
            <ecNumber evidence="4">1.1.1.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gyaR</name>
        <name evidence="8" type="ORF">A3L08_08770</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>glycolate + NAD(+) = glyoxylate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29805"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.26"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GyaR subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.26" evidence="4"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854649.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9E0"/>
    <dbReference type="GeneID" id="33316360"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08770"/>
    <dbReference type="OrthoDB" id="34275at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047964">
        <property type="term" value="F:glyoxylate reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016618">
        <property type="term" value="F:hydroxypyruvate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05301">
        <property type="entry name" value="GDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000462">
        <property type="entry name" value="Glyoxylate reductase (NADP+)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00776">
        <property type="entry name" value="GyaR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023519">
        <property type="entry name" value="Glyoxylate_reductase_GyaR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009714">
        <property type="entry name" value="PRK13243.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF283">
        <property type="entry name" value="GLYOXYLATE_HYDROXYPYRUVATE REDUCTASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="6">
        <location>
            <begin position="5"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="7">
        <location>
            <begin position="110"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="270"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="288"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="158"/>
            <end position="161"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="180"/>
            <end position="182"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="239"/>
            <end position="241"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="288"/>
            <end position="290"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="334" mass="37850" checksum="C0AF940CACCFFD42" modified="2017-09-27" version="1">MKPKVFITRNIPENGIKLLREHFEVEVWEGEHEIPRELFLEKARDADALVTMVSERVDKEVFENAPRLRIVANYAVGYDNIDIGEATKRGIYVTNTPDVLTNATADFAWTLLLATARRLVEADNFVRSGEWKRRGVAWHPLMFLGYDVYGKTIGIVGFGRIGQAIARRAKGFGMRILYNSRTRKPEVEKELGAEFRPLEELLRESDFVVLAVPLTKETYHMINEERLRLMKPTAILVNIARGKVVDTEALVKALKEGWIAGAGLDVFEEEPYYHEELFKLKNVVLAPHIGSATHGAREGMAELVARNLIAFKNGEVPPTLVNREVVKVRKPGFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9E1</accession>
    <name>A0A218P9E1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AbiEi antitoxin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_08695</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854635.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9E1"/>
    <dbReference type="GeneID" id="33316345"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08695"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="24425" checksum="A754C058AAED848C" modified="2017-09-27" version="1">MKLITQFILSRYGGKVITRDELEEICRRFGESLEYIVNYYISRGYLIRILRGVYYVKTLEEYKFGKTPDPLTLISKAMNKLTLKWYFGLYTALKLNGATYEYYSRIFLITPVITRPKPVTILGERVQFVKLKGSLLGFGVVKRREIQYSDLEKTLLDFIYLKRYNKRLNADAVVREYFAKARKEKLIEYSKAYPKSVQREVEAIVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9E4</accession>
    <name>A0A218P9E4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Probable translation initiation factor IF-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">infB</name>
        <name evidence="11" type="ORF">A3L08_08785</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8 9">Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9E4"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08785"/>
    <dbReference type="OrthoDB" id="30957at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03703">
        <property type="entry name" value="aeIF5B_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16266">
        <property type="entry name" value="IF2_aeIF5B_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000013">
        <property type="entry name" value="eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000112">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10050:FF:000009">
        <property type="entry name" value="Probable translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="entry name" value="Translation initiation factor IF- 2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00100_A">
        <property type="entry name" value="IF_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029459">
        <property type="entry name" value="EFTU-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004544">
        <property type="entry name" value="TF_aIF-2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00491">
        <property type="entry name" value="aIF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003078">
        <property type="entry name" value="PRK04004.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011418">
        <property type="entry name" value="PRK14845.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF4">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14578">
        <property type="entry name" value="GTP_EFTU_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="Initiation factor IF2/eIF5b, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 8 9" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="10">
        <location>
            <begin position="5"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="14"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="78"/>
            <end position="82"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="132"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ07407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="598" mass="66240" checksum="45CA4A92FD2A34B2" modified="2017-09-27" version="1">MTKKIRQPIIAVLGHVDHGKTTLLDRIRRTKVAAKEAGGITQHIGATEVPIETVRTLAGPLIKLWKGEIKLPGLLFIDTPGHEAFTSLRARGGSLADLAVLIVDINEGFQPQTIESIEILRKNRTPFIVAANKIDRIKGWKVEEDEPFLVNIKKQDPRAQQELETKLWELIGKFYEMGFQANRFDRVQNFTRELAIVPISAKYGIGVPELLVLIAGLSQKYLEEQLKIEVEGPARGTILEVREELGLGTTIDVIVYDGTLRKDDTIVVGGKDKAIVTKIRALLKPKPLDEIRDPRFRFDQVDEVVAAAGIKIAAPGLEEALAGSPVIAARSEEEIEKAKEEILSQIQSVVINTGKVGVIVKADTLGSLEALSKELSEKNIPIRKADVGNISKTDVMEALSVKEEDEKYGVVLGFNVKVNEDAEEVAKAKGVPIFVGNIIYKLIEDYEAWVTEQEEKKKRELLKNVTFPGVIRLYPDERYVFRRSKPAIVGVEVLEGRIRPGVVLIKQNGEKVGVIKSIKNKNDFVQEAKKGDAVAVAIEGAIVGRHIQPGETLYVDLSKNDVILLAKQLKNELDETDIKALKMTAKVKAQQDPFWKAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9F0</accession>
    <name>A0A218P9F0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dinitrogenase iron-molybdenum cofactor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_08520</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854607.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9F0"/>
    <dbReference type="GeneID" id="33316310"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08520"/>
    <dbReference type="OrthoDB" id="25911at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00851">
        <property type="entry name" value="MTH1175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.130">
        <property type="entry name" value="Dinitrogenase iron-molybdenum cofactor biosynthesis domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003731">
        <property type="entry name" value="Di-Nase_FeMo-co_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036105">
        <property type="entry name" value="DiNase_FeMo-co_biosyn_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033913">
        <property type="entry name" value="MTH1175_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051840">
        <property type="entry name" value="NifX/NifY_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33937:SF2">
        <property type="entry name" value="DINITROGENASE IRON-MOLYBDENUM COFACTOR BIOSYNTHESIS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33937">
        <property type="entry name" value="IRON-MOLYBDENUM PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02579">
        <property type="entry name" value="Nitro_FeMo-Co"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53146">
        <property type="entry name" value="Nitrogenase accessory factor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dinitrogenase iron-molybdenum cofactor biosynthesis" evidence="2">
        <location>
            <begin position="13"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="143" mass="14549" checksum="649A276041842A15" modified="2017-09-27" version="1">MRIIVSTVNGGLDDRVNPAFGRTPTFTIVDVENGEIVNVQVLPNPGYSQPRGAGVTAAQFAIDQGADVVIAGQFGPNSYGVLQAAGIRMVSAPANMTVREAVEAFLRGELSGPVMGPEGGGMGRGRGAGGGMGRGHGRGRGGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9G9</accession>
    <name>A0A218P9G9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">RNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_08955</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854682.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9G9"/>
    <dbReference type="GeneID" id="33316397"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08955"/>
    <dbReference type="OrthoDB" id="39583at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043852">
        <property type="entry name" value="DUF5814"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF1">
        <property type="entry name" value="ATP-DEPENDENT HELICASE MJ1401-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19131">
        <property type="entry name" value="DUF5814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="226"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="404"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="832"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="832"/>
            <end position="849"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="872" mass="100381" checksum="BD61EA7EC4DA03F2" modified="2017-09-27" version="1">MLFVVRPGRKKNELEAFFIENEPEKLAQMRNLKADAIYRFIMREGRLFKVLEGSQYRNPKEMEKLLRQARIVLVNADEWEDYFKRRLQNKRVERAELCRLCLMEGRITVLTEGNRVKYHNEYICERCAEDELKRELRFRFKSVAMFDQAKKLLERFRDLDKVLYAFDPRFDPTRHPEVTKWDELKAKHVKVDKVKVDELPVPEKFKSVLKAEGVNELLPVQSLAVKNGLLEGENLLVVSATASGKTLIGELAGVPKAMKGQKMLFLVPLVALANQKYEDFKRRYSKLGLRVAIRVGMSRIKTKDELVVVDTGIDADIIVGTYEGIDYLLRAGRKIGNVGTIVIDEIHTLDDEERGSRLDGLIARLRKLYPKAQFIGLSATVGNPEELAKELGLKLVLYDERPVDLERHIIIARNEGEKWRHIANLCKAEANRKSKQGYKGQTIVFTFSRKRTHELAAYLTSKGLRAKPYHSGLPYKQRKLTEMEFLAQMLDVVVTTAALGAGVDFPASQVIFESLAMGNKWLSVREFHQMLGRAGRPLYHEKGRVYLIVEPGRKYSAQMEGSEDEVAFKLLTAPIEPVVVEWSDELEQDNVLAHSCVFNRLDVIEEVQSRCLGANQSAEKVLEKLEEFDFVRLNGSLVEVTHYGRAVSMSFLLPKEATFIRENLGEKPAQWIAVKLLPFENLYLSSVLQRELESAVRGRLSANVFSPSFASILDELDKVIPELSPNAAERLFTLYQEFFMCPEDDCTDYAMERVSNMIIELRRSGKHPTQIAEHFRKVYGLIVYPGDVFTWLDGIVRKLEAIERIARVFRVRDAEEEAKILKREIEEGRTIRREGGERRNHPGHQDLRNQRGQSLQNWKGKGRKNWEKRRDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9H1</accession>
    <name>A0A218P9H1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">tRNA(Met) cytidine acetyltransferase TmcA</fullName>
            <ecNumber evidence="14">2.3.1.193</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">tmcA</name>
        <name evidence="16" type="ORF">A3L08_08880</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP).</text>
    </comment>
    <comment type="function">
        <text evidence="14">Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) sites in rRNA, tRNA, mRNA and non-coding (nc)RNA, almost always on the middle C of a CCG motif. In hyperthermophiles more acetylation is seen at higher temperatures.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>a cytidine in RNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in RNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:82211"/>
            <dbReference type="Rhea" id="RHEA-COMP:15704"/>
            <dbReference type="Rhea" id="RHEA-COMP:19834"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a cytidine in mRNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in mRNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:58480"/>
            <dbReference type="Rhea" id="RHEA-COMP:15145"/>
            <dbReference type="Rhea" id="RHEA-COMP:15146"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 14">
            <text>a cytidine in tRNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in tRNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53876"/>
            <dbReference type="Rhea" id="RHEA-COMP:13670"/>
            <dbReference type="Rhea" id="RHEA-COMP:13671"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>cytidine(34) in elongator tRNA(Met) + acetyl-CoA + ATP + H2O = N(4)-acetylcytidine(34) in elongator tRNA(Met) + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43788"/>
            <dbReference type="Rhea" id="RHEA-COMP:10693"/>
            <dbReference type="Rhea" id="RHEA-COMP:10694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.3.1.193"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the TmcA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.193" evidence="14"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854670.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9H1"/>
    <dbReference type="GeneID" id="33316382"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08880"/>
    <dbReference type="OrthoDB" id="312894at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990883">
        <property type="term" value="F:18S rRNA cytidine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106162">
        <property type="term" value="F:mRNA N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051392">
        <property type="term" value="F:tRNA cytidine N4-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904812">
        <property type="term" value="P:rRNA acetylation involved in maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051391">
        <property type="term" value="P:tRNA acetylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002101">
        <property type="term" value="P:tRNA wobble cytosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03143">
        <property type="entry name" value="A4_beta-galactosidase_middle_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002218">
        <property type="entry name" value="tRNA(Met) cytidine acetyltransferase TmcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000140">
        <property type="entry name" value="tRNA(Met) cytidine acetyltransferase TmcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01886">
        <property type="entry name" value="tRNA_acetyltr_TmcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007807">
        <property type="entry name" value="TcmA/NAT10_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032672">
        <property type="entry name" value="TmcA/NAT10/Kre33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013562">
        <property type="entry name" value="TmcA/NAT10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024914">
        <property type="entry name" value="tRNA_acetyltr_TmcA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10925">
        <property type="entry name" value="N-ACETYLTRANSFERASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10925:SF5">
        <property type="entry name" value="RNA CYTIDINE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13718">
        <property type="entry name" value="GNAT_acetyltr_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05127">
        <property type="entry name" value="NAT10_TcmA_helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08351">
        <property type="entry name" value="TmcA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 14" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6 14" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="4 14 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 14" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3 14" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="15">
        <location>
            <begin position="474"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="590"/>
            <end position="592"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="630"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ASJ07425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="810" mass="93539" checksum="817DF88954E4CEE6" modified="2017-09-27" version="1">MTVKVRFDKDVREYAKGEKVKDEVLKLTETALAQALEKFHRRMIVIEGDTERKAELAGILAGASARVLSEVLDELMKKRLRDESESAIEVLYATDALGEDTFGRKRYEAFRKHFDVLAGSEANVQAVTFKHTRDILGRTYDLLILDMSYDYSPNDLGRIIETVRGGGLIFILAHPFKKWKDMWTGFHKSLVTPPYTIDDVKKRFNRRLIRKFTEYGEIYIITENGKIRKKPKRSKSQAKIKGRKGVPIPEETLFPRELYEMALTEGQVEVLKAFEDLVENEGMLVLTADRGRGKSVSVGIAAVGLALALKKRTRIVVTAPEPENVQSLFRFAKRALERLGFKPYVVEEKGLIKELYARKIGLRYYPPAEGYKKSADLYILDEAAGIHVPILHKYLNKPRVVYSSTVHGYEGAGRGFSVKFLKKAREKREFRELHMDEPIRYADHDPIEKWLFDVLLLDAEPVELTEEDYELIKRKEVYFEEPDLDDWFEHDREDLRHFVGIYILAHYRNRPSDVALLADAPHHEARVLRLKNGKIVTAVQIAKEGNIPKNVIEKMAKGYKPRGNIIPDMMVKHHMLKEFAKLKGYRIVRIATHPDAMDMGLGSKALELLEKEAREKGLDWIGSGFGASEELARFWVKNGFAVVHLSPARNPVSGEFTAIVLKPISEKAKKLIKQANDEFRIRLTEWLGDTHRELEPEIARWLFETPFGEAVEYPVHLTDVQKKRLDAFTGKVLTYDTVVDAVKPVVKLYFLDGWMKPYLDERQIKLLIYRVLQAHSWEETAKLIDRTEMFTMIEVRDIIRGLWYYYKRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9I7</accession>
    <name>A0A218P9I7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Tyrosyl-tRNA synthetase</fullName>
            <shortName evidence="8">TyrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tyrS</name>
        <name evidence="10" type="ORF">A3L08_08965</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854684.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9I7"/>
    <dbReference type="GeneID" id="33316399"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08965"/>
    <dbReference type="OrthoDB" id="8389at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02009">
        <property type="entry name" value="Tyr_tRNA_synth_type4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023678">
        <property type="entry name" value="Tyr-tRNA-ligase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023617">
        <property type="entry name" value="Tyr-tRNA-ligase_arc/euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050489">
        <property type="entry name" value="Tyr-tRNA_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006330">
        <property type="entry name" value="PRK08560.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264:SF4">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264">
        <property type="entry name" value="TYROSINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006588">
        <property type="entry name" value="TyrRS_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="251"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ07439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="375" mass="43171" checksum="9D41824408B9CC53" modified="2017-09-27" version="1">MDIESRIELIKKKPTEELLTEENLRHLLEVGIPMQHYIGFEISGYIHLGTGLMAGAKIADLQKAGVKTRIFLADWHSWINDKLGGDLDVIQKVALTYFKEGMKQSIKVMGGDPDKVEFVLASEILEKGDYWQTVIDISKNVTLARMMRSITIMGRQMGEAIDFAKLIYPAMQVADIFYQGVTIAHAGMDQRKAHVIAIEVAQKLKYHALEWNGEKLKPVALHHHLLLGLQEPPVWPIESEEQFKELKTQMKMSKSKPYSAVFIHDTPEEIRQKLRKAFCPAKEVNYNPVLDWAEHIIFREEPTEFTIHRPAKFGGDITYTTFEELKRDFAEGKLHPLDLKNAVAEYLIELLKPIRDYFERHPEPLELMNQVKITR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9K2</accession>
    <name>A0A218P9K2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Urea amidolyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_09110</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854710.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9K2"/>
    <dbReference type="GeneID" id="33316428"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09110"/>
    <dbReference type="OrthoDB" id="85703at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003778">
        <property type="entry name" value="CT_A_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052708">
        <property type="entry name" value="PxpC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00724">
        <property type="entry name" value="urea_amlyse_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43309">
        <property type="entry name" value="5-OXOPROLINASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43309:SF3">
        <property type="entry name" value="5-OXOPROLINASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02626">
        <property type="entry name" value="CT_A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00797">
        <property type="entry name" value="AHS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxyltransferase" evidence="4">
        <location>
            <begin position="23"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="334" mass="36363" checksum="19653D84BAF985C4" modified="2017-09-27" version="1">MIELLKVPSLLTVQDSGRKGYRKLGVPVSGYMDDYSARIANYLVGNPGDAPLLEFLLAGPTLRFNASAVFAVAGDVDLKLNGLPIEPWESHWAKRGDVLEVGTLRSGLYGYIAFAGGIKCEKLLGSCSTYSRANLGRPLKAGDRLTLGYAILAGKEGRSLHRDLRPDYSGNEREIRVLLGPNLEHFTEEGVGTFISETYTVTPESDRMGYRFDGPAIGHSSLGADIVTEPLVPGAVQVPANGKPIVMMRDAQTTGGYAKIATVISADLHLLAQSRPGTRVRFKAVNVEEAQEILRRCEKTLRAIEEFLAGKMKSYRIRTFGEEKIAFTKVEKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9K7</accession>
    <name>A0A218P9K7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glycerol-1-phosphate dehydrogenase [NAD(P)+]</fullName>
            <shortName evidence="11">G1P dehydrogenase</shortName>
            <shortName evidence="11">G1PDH</shortName>
            <ecNumber evidence="11">1.1.1.261</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Enantiomeric glycerophosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">sn-glycerol-1-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 15" type="primary">egsA</name>
        <name evidence="15" type="ORF">A3L08_09100</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol 1-phosphate (G1P). The G1P thus generated is used as the glycerophosphate backbone of phospholipids in the cellular membranes of Archaea.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>sn-glycerol 1-phosphate + NAD(+) = dihydroxyacetone phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.261"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>sn-glycerol 1-phosphate + NADP(+) = dihydroxyacetone phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21416"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.261"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11 12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the glycerol-1-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.261" evidence="11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9K7"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09100"/>
    <dbReference type="OrthoDB" id="8656at2157"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106357">
        <property type="term" value="F:glycerol-1-phosphate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106358">
        <property type="term" value="F:glycerol-1-phosphate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08173">
        <property type="entry name" value="Gro1PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00497_A">
        <property type="entry name" value="G1P_dehydrogenase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023002">
        <property type="entry name" value="G1P_dehydrogenase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032837">
        <property type="entry name" value="G1PDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016205">
        <property type="entry name" value="Glycerol_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002022">
        <property type="entry name" value="PRK00843.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43616">
        <property type="entry name" value="GLYCEROL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43616:SF5">
        <property type="entry name" value="GLYCEROL DEHYDROGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13685">
        <property type="entry name" value="Fe-ADH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000112">
        <property type="entry name" value="Glycerol_dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11 14" id="KW-0520">NAD</keyword>
    <keyword evidence="5 11" id="KW-0521">NADP</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9 11" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11 12" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="11 14">
        <location>
            <begin position="100"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 14">
        <location>
            <begin position="122"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>glycerol</name>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 14">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>glycerol</name>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>glycerol</name>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>glycerol</name>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000112-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000112-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000112-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ASJ07464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="353" mass="37846" checksum="872D4637A11E0D18" modified="2017-09-27" version="1">MSRVVREVHLMQLPREVLLGENLVGEVVNVANRLGLGRKALVLYGPRTKKIAGKDVEDVLREEYEVEGVTVKGATMEEVERVLSIIGDHGSDWLIAVGGGSIIDVAKLASFKAGVPFISFPTTASHDGIASANASIKDLGSKTSVKAVPPIAVIADVKVIKTAPYRYLAAGVGDMISNLTAVRDWQLAHRIKGEYYSEYAASLSLMSAKMVIKNADIIRLGNEESVRKVVKGLISCGVAMSIAGSSRPASGAEHLFSHALDMIAPKPALHGEQVGVGTIIMAYLHGLKWERVRETLKRVGAPTNAYELGIEPELVIEALTIAHTIRPERYTILGKDGLTREAAEKAAKITGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9L1</accession>
    <name>A0A218P9L1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Prokaryotic stomatin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_09165</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854720.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9L1"/>
    <dbReference type="GeneID" id="33316439"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09165"/>
    <dbReference type="OrthoDB" id="10752at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08826">
        <property type="entry name" value="SPFH_eoslipins_u1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000004">
        <property type="entry name" value="Putative membrane protease family, stomatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2090">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043202">
        <property type="entry name" value="Band-7_stomatin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264:SF19">
        <property type="entry name" value="AT06885P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264">
        <property type="entry name" value="BAND 7 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Band 7" evidence="9">
        <location>
            <begin position="24"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="179"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00080748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ07476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="279" mass="31101" checksum="11ECF1C875F59AD8" modified="2017-09-27" version="1">MALANVSNVVLAIVLLFVLIILATAIKIVKEYERAVIFRLGRVVGARGPGLFFIIPIFEKAVIVDLRTRVLDVPVQETITKDNVPVRVNAVVYFRVIDPVKAVTQVANYIMATSQIAQTTLRSVIGQAHLDELLSEREKLNLQLQKIIDEATDPWGIKVSTVEIKDVELPSGMQRAMARQAEAERERRARITLAEAERQAAEKLREAAEIISEHPMALQLRTLQTISDVASDKSNVIILPLPMEMLKLFRSLSDSAEAAKLFFKNRAAKDSEEKHDAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9L7</accession>
    <name>A0A218P9L7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Endonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854725.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9L7"/>
    <dbReference type="GeneID" id="33316445"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09195"/>
    <dbReference type="OrthoDB" id="17296at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10441">
        <property type="entry name" value="GIY-YIG_COG1833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002837">
        <property type="entry name" value="DUF123"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37460">
        <property type="entry name" value="ENDONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37460:SF1">
        <property type="entry name" value="ENDONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01986">
        <property type="entry name" value="DUF123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00465">
        <property type="entry name" value="GIYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GIY-YIG" evidence="1">
        <location>
            <begin position="18"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="147" mass="17310" checksum="FB91BF022CC77428" modified="2017-09-27" version="1">MKGSYFLVIRLGRDRNVRTRGREFRLKEGYYVYVGSAMNSLEKRVARHFSKEKKLHWHIDHLLNEAELLRAYLVPSEERLEERLSLEVARYGEPVNGFGAGDIKLNTNLYRFEEEPDDVLRAILEGLGLKWKRVKSREEVIEFGGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9M4</accession>
    <name>A0A218P9M4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">5-oxoprolinase subunit A</fullName>
            <shortName evidence="2">5-OPase subunit A</shortName>
            <ecNumber evidence="2">3.5.2.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">5-oxoprolinase (ATP-hydrolyzing) subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pxpA</name>
        <name evidence="3" type="ORF">A3L08_09120</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the cleavage of 5-oxoproline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>5-oxo-L-proline + ATP + 2 H2O = L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10348"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58402"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.5.2.9"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Forms a complex composed of PxpA, PxpB and PxpC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LamB/PxpA family.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.9" evidence="2"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854712.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9M4"/>
    <dbReference type="GeneID" id="33316430"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09120"/>
    <dbReference type="OrthoDB" id="84497at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10787">
        <property type="entry name" value="LamB_YcsF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00691">
        <property type="entry name" value="PxpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005501">
        <property type="entry name" value="LamB/YcsF/PxpA-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003814">
        <property type="entry name" value="PRK05406.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003816">
        <property type="entry name" value="PRK05406.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30292:SF0">
        <property type="entry name" value="5-OXOPROLINASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30292">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YBGL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03746">
        <property type="entry name" value="LamB_YcsF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="255" mass="28212" checksum="EDDB3D58F5C16DBA" modified="2017-09-27" version="1">MKVDLNSDLGESFGRYKLGLDEEVMKYISSANIATGWHAGDPIVMRKTVRLAKENSVAVGAHPGYPDLLGFGRRYMKLSPEEARNYILYQVGALYAFARAEGLELQHVKPHGALYNALVKEEELARAVIEGIADFDRKLIFVTLSGSRPAEIAEEMGVKVAHEVFADRAYNPDGTLVPRSKPGAVIHDKDEIAERVVSMVKDGGVRAINGEWVELKADTICVHGDNPKAVEIAKYIRDVLEREGVKIVPMKEIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9N7</accession>
    <name>A0A218P9N7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Peptidase M42</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_09245</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="9">Binds 2 divalent metal cations per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase M42 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854731.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9N7"/>
    <dbReference type="GeneID" id="33316455"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09245"/>
    <dbReference type="OrthoDB" id="30642at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05656">
        <property type="entry name" value="M42_Frv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.40">
        <property type="entry name" value="Peptidase M42, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008007">
        <property type="entry name" value="Peptidase_M42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051464">
        <property type="entry name" value="Peptidase_M42_aminopept"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023367">
        <property type="entry name" value="Peptidase_M42_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32481">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32481:SF0">
        <property type="entry name" value="AMINOPEPTIDASE YPDE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05343">
        <property type="entry name" value="Peptidase_M42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001123">
        <property type="entry name" value="PepA_GA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101821">
        <property type="entry name" value="Aminopeptidase/glucanase lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR001123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ07488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="348" mass="38144" checksum="CA990E179B97D8B5" modified="2017-09-27" version="1">MVDFELLKRIIEAPGVTGYEFLGVRDVVIEAFKPYVDEIKVDKLGNVIAHKKGSGPKVMLAGHMDQIGLMVTHIEKNGFLRVAPVGGVDPRTLIAQRFKVWVGPNEYIYGVGGSVPPHIQKPEERSKAPTWDQVFIDVGAESKEEAEEMGVKIGTVITWDGRLERLGKHRLVSIAHDDRIAVYILVEAARQLAKTDADVYFVATVQEEVGLRGAKVSAFGIDPDYGFALDVTIAADVPGTPEHKQVTQLGKGVAIKIMDRSVICHPTIVRWMEELAKKHEIPYQWDILLGGGTDAGAIHLNKAGVPTGGISIPARYIHSNTEVVDERDVDAAVKLTVKVLEEIPELKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Q0</accession>
    <name>A0A218P9Q0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Flap endonuclease 1</fullName>
            <shortName evidence="12">FEN-1</shortName>
            <ecNumber evidence="12">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Flap structure-specific endonuclease 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">fen</name>
        <name evidence="15" type="ORF">A3L08_09450</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12">Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11 12">Interacts with PCNA. PCNA stimulates the nuclease activity without altering cleavage specificity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="12"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854770.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Q0"/>
    <dbReference type="GeneID" id="33316496"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09450"/>
    <dbReference type="OrthoDB" id="9593at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09903">
        <property type="entry name" value="H3TH_FEN1-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09867">
        <property type="entry name" value="PIN_FEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000087">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000016">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00614">
        <property type="entry name" value="Fen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023426">
        <property type="entry name" value="Flap_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019973">
        <property type="entry name" value="Flap_endonuc_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03674">
        <property type="entry name" value="fen_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF9">
        <property type="entry name" value="FLAP ENDONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 12 15" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 12" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="14">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="13">
        <location>
            <begin position="140"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-domain" evidence="12">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with PCNA" evidence="12">
        <location>
            <begin position="330"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ASJ07526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="341" mass="38970" checksum="6FE2F693732A0373" modified="2017-09-27" version="1">MGVQIGELVPRKEIELQNLYGKKVAIDAFNAMYQFLSTIRQRDGTPLMDSKGRITSHLSGFFYRTINLMEAGIKPAYVFDGKPPEFKKKEIEKRREAREEAEEKWHEALEKGDLEEAKKYAMRATRVNEQLINDAKKLLELMGIPVVQAPSEGEAQAAYMAAKKKVYASASQDYDSLLFGAPKLVRNLTITGRRKLPGKNVYVEVKPELIVLEEVLKELGIDREKLIELAILVGTDYNPSGIKGIGPKKALTIVKRTKDPLKKYQKESEVDLYAIKEFFLNPPVTDEYELRWREPDEEGILKFLCDEHDFSEERVKSGLERLKKAVESGKQRTLESWFGKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Q4</accession>
    <name>A0A218P9Q4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_09385</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854758.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Q4"/>
    <dbReference type="GeneID" id="33316483"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09385"/>
    <dbReference type="OrthoDB" id="132546at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050194">
        <property type="entry name" value="Glycosyltransferase_grp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947:SF3">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="2">
        <location>
            <begin position="14"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="1">
        <location>
            <begin position="174"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="343" mass="37648" checksum="75B39CE15BF82E21" modified="2017-09-27" version="1">MRILMVGHYPPHGGGVANHLDSLVGELRKRHEVHVLTYGPVEPRGFEREFVHQVRVPPVYGFRGTSFAFLGSREIVKLHRELDFDIVHAHFVGTTSFAGVLAKGKTGIPLVVTAHGSDLEHTAKLALGRFYVKKTLLEADAVITVSHWLAKKALSLGASSVRVIPNGVRALTPGKEGKREFVTFIGALREYKSPETFIELARRFPEREFLVVGDGPLRKTLEATAPENVHFLGYRNDIGNILSKSALLVLPSKREGFGLVILEANSLGVPAAGRRVSAIPELIREGKNGLTFGTFEELVEAVGLLLEPKRNRKTGLIAGRVAALYTWEEVAREVEGVYRSVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9R5</accession>
    <name>A0A218P9R5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">molybdopterin molybdotransferase</fullName>
            <ecNumber evidence="4">2.10.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">A3L08_09415</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>adenylyl-molybdopterin + molybdate = Mo-molybdopterin + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35047"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
            <dbReference type="ChEBI" id="CHEBI:62727"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.10.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MoeA family.</text>
    </comment>
    <dbReference type="EC" id="2.10.1.1" evidence="4"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854763.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9R5"/>
    <dbReference type="GeneID" id="33316489"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09415"/>
    <dbReference type="OrthoDB" id="31371at2157"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.190.11:FF:000001">
        <property type="entry name" value="Molybdopterin molybdenumtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.980.10:FF:000004">
        <property type="entry name" value="Molybdopterin molybdenumtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.340.10:FF:000005">
        <property type="entry name" value="Molybdopterin molybdenumtransferase MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="entry name" value="MoeA, C-terminal, domain IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.10">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.190.11">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045515">
        <property type="entry name" value="Glp_gephyrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192:SF5">
        <property type="entry name" value="GEPHYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="MOLYBDOPTERIN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="MoeA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="MoeA N-terminal region -like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="9" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="11">
        <location>
            <begin position="184"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ07519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="400" mass="42803" checksum="1D6205E14237880F" modified="2017-09-27" version="1">MAFLKVVPLEKALEVINSFPLEPKVEKVPLEEALGRVLAEDVVSPIDVPPFDRATVDGYAVRAEDTFMASESEPVRLKVVGEINAGDTPNIELKPGEAVYISTGAPLPKGADAVIQFEDVSRDGDEVIIYKPAYPSLGVMKAGADIPKGEPLLKRGTRLTFKDTALLSAVGIAEVPVFRKPKVAVISTGNEIVPPGSELRHGQIYDINGRAITDAVRELGGEAFFLGIARDDRENLKALIEKGVECCDVVLLSGGASGGIRDLTNSIVEELGEVKIHGIAIQPGKPTVIGLIDGKPVFGLPGYPTSCLTNFTLLVAPLLRKLIGREGEVRKVKKRLAHKVFSVKGRRQFLPVKIDGEKAVPILKGSGAVTSFVDADGFIEVPENVEILEAGEEVEVTFFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9T6</accession>
    <name>A0A218P9T6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">AAA family ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_09540</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family. CDC48 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854787.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9T6"/>
    <dbReference type="GeneID" id="33316514"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09540"/>
    <dbReference type="OrthoDB" id="77269at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19511">
        <property type="entry name" value="RecA-like_CDC48_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000007">
        <property type="entry name" value="AAA family ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.10:FF:000008">
        <property type="entry name" value="AAA family ATPase, CDC48 subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000178">
        <property type="entry name" value="CDC48/VCP homolog, AAA superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000018">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005938">
        <property type="entry name" value="AAA_ATPase_CDC48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01243">
        <property type="entry name" value="CDC48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="8">
        <location>
            <begin position="8"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48" evidence="7">
        <location>
            <begin position="107"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="215"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="550"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="387"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="796" mass="88811" checksum="EC29DA5F21940AC8" modified="2017-09-27" version="1">MAERREVKLKVASAYQRDVGRGIVRIDRKAMREIGVQSGDIIEIIGTKNTAAVVWPAYPEDEGLGIIRMDGTLRKNAGVGLGDEVTIRKAEVKEAKKVIVAPTEPIRFGGDFVEWLHSRLVGRPVVRGDYIKVGILGQELTFVVTATTPAGIVQITEFTDFQVSEKPVKEVSKTAALGVTYEDIGGLKDVIQKVREMIELPLKHPEIFEKLGIEPPKGVLLYGPPGTGKTLLAKAVANEANAHFIAINGPEIMSKYYGESEERLREVFKEAEENAPAIIFIDEIDAIAPKREEVSGEVEKRVVSQLLTLMDGLKSRGKVIVIGATNRPDAIDPALRRPGRFDRELEVGVPDKQGRKEILQIHTRGMPIEPEFRKSKVIEILEELERNETYRDAAEKAIAKVKKAESEEEIKKALREVEERLYDEVKAKLIDSLLEELAEVTHGFVGADLAALAREAAMAALRRLIQEGKIDFEAEHIPKEVLEDLKVTRRDFYEALKMIEPSALREVLLEVPNVHWDDIGGLEDVKEELREAVEWPLKYPEAFMGLGINPPKGILLYGPPGTGKTLLAKAVANESEANFIAIKGPEVLSKWVGESEKNIREIFRKARQAAPTVIFIDEIDAIAPRRGTDVNRVTDRLINQLLTEMDGIQENSGVVVIGATNRPDIIDPALLRPGRFDRLILVPAPDEKARLEIFKVHTRRVPLAEDVSLEELAKRTEGYTGADIEAVVREAAMLAMRRALQEGIIRPGMKADEIRQKVKVTMKDFEEALKKIGPSVSKETMEYYRKVQEQFKQARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9U0</accession>
    <name>A0A218P9U0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">mRNA 3'-end processing factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09530</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854785.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9U0"/>
    <dbReference type="GeneID" id="33316512"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09530"/>
    <dbReference type="OrthoDB" id="40950at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050698">
        <property type="entry name" value="MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203:SF37">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="285" mass="32407" checksum="4D13A61C41B175A8" modified="2017-09-27" version="1">MIIGKVGLDSSARFTFQSHAHTDHFVSGEVIFATRATKYLSHLRKGGFYREIDFGKTFYLGDLKAKLYPAGHMLGSAGIKLWLEGGTLFYTGDTKWFKLRTAEKSRFPRADFLIIEATFGVPRFTFPSPREAEKKLVAFVEETLDRGKRPVLYVNQMGKAQEVMKILDVHGYTVRPSGTMLKVAKVYSKFGIKFGNVDKDGEVVLRSYRSLKVENSFSPWELTVSGFGRLKLSNHADFWELMRIIEKIKPEKIFTVYGFADEFARILRGLGYDALPIKSDSELKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9X1</accession>
    <name>A0A218P9X1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09765</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854828.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9X1"/>
    <dbReference type="GeneID" id="33316559"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09765"/>
    <dbReference type="OrthoDB" id="9014at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004061">
        <property type="term" value="F:arylformamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.50">
        <property type="entry name" value="Putative cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007325">
        <property type="entry name" value="KFase/CYL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037175">
        <property type="entry name" value="KFase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31118">
        <property type="entry name" value="CYCLASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31118:SF12">
        <property type="entry name" value="CYCLASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04199">
        <property type="entry name" value="Cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102198">
        <property type="entry name" value="Putative cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="192" mass="20427" checksum="814C937ADA358852" modified="2017-09-27" version="1">MIVDLSMPISEETPVYPDDPPVGIKLWAVIDRDGYYMNALKMGEHTGTHVDAPAHFIPGGKTVDEMPLEKFIGEGLVLDVRGGEGEIKLDEIPDSGYFDRVVLFLTGGRGLSPEVALFIVAEGAKAVGTDAMSIGDDAVHKILLSAEVPVFENLTNLEALLGKEFTFIALPLKIEGGSGSPVRAVAVIHSGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA00</accession>
    <name>A0A218PA00_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent DNA helicase Hel308</fullName>
            <ecNumber evidence="10">5.6.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">DNA 3'-5' helicase Hel308</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">hel308</name>
        <name evidence="13" type="ORF">A3L08_00270</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">DNA-dependent ATPase and 3'-5' DNA helicase that may be involved in repair of stalled replication forks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the helicase family. Hel308 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="10"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854842.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA00"/>
    <dbReference type="GeneID" id="33314658"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00270"/>
    <dbReference type="OrthoDB" id="371946at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18028">
        <property type="entry name" value="DEXHc_archSki2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00442">
        <property type="entry name" value="Helicase_Hel308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048772">
        <property type="entry name" value="Hel308-like_dom4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022965">
        <property type="entry name" value="Helicase_Hel308"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001308">
        <property type="entry name" value="PRK00254.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF10">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE HEL308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21280">
        <property type="entry name" value="Helicase_dom4_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14520">
        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 10 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="33"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="226"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ07598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="726" mass="83120" checksum="B5F762A589174564" modified="2017-09-27" version="1">MKVDELPVDERIKKIIRERGIEELYPPQAEALKSGVLEGKNLVLAIPTASGKTLVSEIVMINKLLREGGKAVYLVPLKALAEEKYREFKEWESLGVRVAATTGDYDSTDEWLGRYDIIVATAEKFDSLLRHSPGWIKDVKLIVADEVHLIGSYDRGATLEMILSHMLGRAQILALSATVGNAEELAEWLDAALVMSDWRPVELRKGVFHLGQLFWEDGKIDRYPENWESLAIDAVKKGKQALVFVNTRRSAEKEAVSLSSKVSRLLTKPETRQLEELTSQIEDNPTTEKLKRALRGGVAFHHAGLSRAERTLIEDAFRDGLIKVITATPTLAVGVNTPAFRVIIRDTKRYAGFGWTDIPVLEIQQMMGRAGRPKYDRYGEAIIVARTDDPKNLMERYVHGKPEKLFSMLANEQAFRSQILALITNFGILNFRELVSFLERTFYAHQRGDIASLEYKAKNVVYFLIENEFIDMDVEDRFMALPFGRRTSQLYIDPVTAKKFRDAFPMLEKNPNPFGIFQLIASTPDMATLTARKRELEDYLDLAYEFEEKLYTNIPYYEDSRFQGFLSQVKTAKVLLDWINEVPETRIYETYNIDPGDLYRILELADWLMYALIELYRLFEPKEDVLNYLRDLHLRLRHGVREELLELVRLPNIGRKRARALYNAGFRTPEDIMRAKVKDLLEVEGIGIKVVEGLFKHFGVEMPGKVKDAGKKAERKKKGTLDAFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA23</accession>
    <name>A0A218PA23_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidyl-prolyl cis-trans isomerase</fullName>
            <ecNumber evidence="9">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_02195</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the FKBP-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="9"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854864.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA23"/>
    <dbReference type="GeneID" id="33315044"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02195"/>
    <dbReference type="OrthoDB" id="8615at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47861">
        <property type="entry name" value="FKBP-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE SLYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47861:SF3">
        <property type="entry name" value="FKBP-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE SLYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="10">
        <location>
            <begin position="6"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ07622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="163" mass="17710" checksum="AEAA191C512068F1" modified="2017-09-27" version="1">MKIEAGDFVAFHYVGRFENGEVFDTSYEDIAKENGIFVEEREYGPLGVNVGVGEIIPGLDEALIGMEIGEKKTVVVPPEKAYGMPNPELIIDVPISEFTQAGLEPVEGMYVMTDSGIAKIARIEGESVSLDFNHPLAGKTLVFEVEIVDIQKGEEKDSGEAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA24</accession>
    <name>A0A218PA24_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_02315</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854867.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA24"/>
    <dbReference type="GeneID" id="33315068"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02315"/>
    <dbReference type="OrthoDB" id="131038at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="2">
        <location>
            <begin position="160"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="347" mass="40057" checksum="73D1A33038025BFF" modified="2017-09-27" version="1">MKIIALPAFRNRKINPYNSLLYENLIALGIRVEEFSVWKLVRGGYDIWHIHWPELVLNTPSYLMATLRAKAFLSLMGIARKRGIKIVWTVHNLRSHEGFHPKLEERFWERFLGMVDGHISLTSIGQEELFRTYPQLRRVPGFVIPHGHYRDVYPNNISRKQARALLGLPMDSRVILFIGQIRKYKNLPSLIRAFNSLPEKDVRLVIAGKPVDRELTNQLFKLVKSDSRIMFHPKFIPSEEIQVYLNSADLVVLPYSEILNSGSALLALSFDRPVLAPAKGSMIELRDSVGKEWVMLYTGELTGDTLSNALEWAILEKRPARAPLDRFSWDNIARETLKAYKKVLERE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA32</accession>
    <name>A0A218PA32_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Adenosylcobinamide amidohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05950</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA32"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05950"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002808">
        <property type="entry name" value="AdoCbi_amidolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052209">
        <property type="entry name" value="CbiZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35336">
        <property type="entry name" value="ADENOSYLCOBINAMIDE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35336:SF5">
        <property type="entry name" value="ADENOSYLCOBINAMIDE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01955">
        <property type="entry name" value="CbiZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="182" mass="19344" checksum="8BBDC77DA8E3D583" modified="2017-09-27" version="1">MLSLSNAPHRGGLTRADGFFFMMVPKNYSGDYKADCLAFEREQGLKNFVGFMTAADIGAVLSTAKSGSVSAYITAGITNPAIAGEVPPPWRPGTINIALVIDEGLTVGAMANAIMTATEAKTYTLLRLGYKATGTTSDGIGIFAFEGEKEWAGTATELGINIGRAVRKALEESLRKWEKTRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PAA1</accession>
    <name>A0A218PAA1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Putative adenylate kinase</fullName>
            <shortName evidence="7">AK</shortName>
            <ecNumber evidence="7">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_09130</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Has also ATPase activity. Involved in the late maturation steps of the 30S ribosomal particles, specifically 16S rRNA maturation. While NMP activity is not required for ribosome maturation, ATPase activity is. Associates transiently with small ribosomal subunit protein uS11. ATP hydrolysis breaks the interaction with uS11. May temporarily remove uS11 from the ribosome to enable a conformational change of the ribosomal RNA that is needed for the final maturation step of the small ribosomal subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with uS11. Not a structural component of 40S pre-ribosomes, but transiently interacts with them by binding to uS11.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adenylate kinase family. AK6 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="7"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854940.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PAA1"/>
    <dbReference type="GeneID" id="33316432"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09130"/>
    <dbReference type="OrthoDB" id="8730at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00039">
        <property type="entry name" value="Adenylate_kinase_AK6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020618">
        <property type="entry name" value="Adenyl_kinase_AK6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003012">
        <property type="entry name" value="PRK03839.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595:SF0">
        <property type="entry name" value="ADENYLATE KINASE ISOENZYME 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595">
        <property type="entry name" value="POS9-ACTIVATING FACTOR FAP7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13238">
        <property type="entry name" value="AAA_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="NMP" evidence="7">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="7">
        <location>
            <begin position="99"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="177" mass="19621" checksum="17618DB779CC407A" modified="2017-09-27" version="1">MIIAVTGTPGVGKTTVSKLLAERLKYGYVNLRDYALEKGLGEMKGDELELDVDELAENFARDFSGKDVVVDSHLAHYMPADIVVVLRAHPRLVGERLKARGYSKEKVGENVEAELVDVILVEALEENENVIEVDTTGKTPEEVVEEILGLLEKGVKRRVGVVDWSGVYDEVVPYLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2015-03-04" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0A7RBQ4</accession>
    <name>A0A0A7RBQ4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Beta-galactosidase</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="5">Beta-glucosidase</fullName>
            <ecNumber evidence="5">3.2.1.21</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_06935</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2014-09" db="EMBL/GenBank/DDBJ databases">
            <title>Novel substrate specificity of a thermostable beta-glucosidase from the hyperthermophilic archaeon, Thermococcus pacificus.</title>
            <authorList>
                <person name="Kim Y.J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">P-4</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="5"/>
    <dbReference type="EMBL" id="KM583848">
        <property type="protein sequence ID" value="AJA30078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854325.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0A7RBQ4"/>
    <dbReference type="SMR" id="A0A0A7RBQ4"/>
    <dbReference type="GeneID" id="33315990"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06935"/>
    <dbReference type="OrthoDB" id="84443at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053427">
        <property type="entry name" value="Beta-galactosidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="GH_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041004">
        <property type="entry name" value="Beta_gal_BgaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF209">
        <property type="entry name" value="GALACTOLIPID GALACTOSYLTRANSFERASE SFR2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AJA30078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="487" mass="56307" checksum="EE4C2BF81D4F1FA2" modified="2015-03-04" version="1">MYKFPKDFLFGYSWSGFQFEMGLPGSEVPNSDWWVWVHDIENIATGLVSGDLPENGPAYWDLYKKDHDIAESLGMDAIRGGIEWARIFPKPTFDVKARVEKDEEGNIVSVEVPESSIKELEKIADMKALEHYREIYADWKERGKTFILNLYHWPLPLWLHDPLKVRKLGPDRAPAGWLDDKSVVEFAKFAAFVAYHLDDLVDSWSTMNEPNVVYQNGYTRPTSGFPPGYLSFEAERKAKMNLIQAHARAYDVIKEYSDKDVGIIYAYTWPDPLREEVEDEVRAIRERELYSFVDAVHFGKAADIEERDDLQGKVDWLGVNYYSRLAFDRVNGHVLPVSGYGFSGERGGYARSGRACSDFGWEIYPEGLEKLLKDLAKRYGLPMMITENGIADAADRYRSHYLVSHLRAVYEAMKEGADVRGYLHWSLTDNYEWAQGFRMRFGLVYVDMGTKKRYLRPSALVFREIATRKEIPEELEHLSSLDFLVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P4W3</accession>
    <name>A0A218P4W3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853097.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P4W3"/>
    <dbReference type="GeneID" id="33314612"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00040"/>
    <dbReference type="OrthoDB" id="308128at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07344">
        <property type="entry name" value="M48_yhfN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053136">
        <property type="entry name" value="UTP_pyrophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002725">
        <property type="entry name" value="YgjP-like_metallopeptidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30399">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YGJP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30399:SF1">
        <property type="entry name" value="UTP PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01863">
        <property type="entry name" value="YgjP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YgjP-like metallopeptidase" evidence="1">
        <location>
            <begin position="11"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="215" mass="25440" checksum="A79DD4C5EA4F0A0B" modified="2017-09-27" version="1">MDLEIRRRPVRYARIEVKPDGRVVVTAPKGFDVESFVERHKGWLEAKLAEIKNLRERTGSGFPIDGELYQVIRGRKTKVHEKFRTVVFSAYPDEAIAELKSYLRPRILALVDEYSREMGVSPKKVFIRHQRSRWGSCSPRGNLNFNVRLVSVPPKLREYVVVHELAHLKYMNHSKAFWNLVGRFYPDYRKARKELKKWWSIIELNPYWRWLGGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P4Z5</accession>
    <name>A0A218P4Z5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Bifunctional IPC transferase and DIPP synthase</fullName>
            <ecNumber evidence="6">2.7.7.74</ecNumber>
            <ecNumber evidence="7">2.7.8.34</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">A3L08_00135</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Involved in biosynthesis of di-myo-inositol phosphate (DIP), a widespread organic solute in microorganisms adapted to hot environments. Catalyzes the condensation of CTP and L-myo-inositol-1-phosphate into CDP-L-myo-inositol, as well as the biosynthesis of di-myo-inositol-1,3'-phosphate-1'-phosphate (DIPP) from CDP-L-myo-inositol and L-myo-inositol-1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1D-myo-inositol 3-phosphate + CTP + H(+) = CDP-1L-myo-inositol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:30647"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
            <dbReference type="ChEBI" id="CHEBI:62573"/>
            <dbReference type="EC" id="2.7.7.74"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>CDP-1L-myo-inositol + 1D-myo-inositol 3-phosphate = bis(1L-myo-inositol) 3,1'-phosphate 1-phosphate + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31327"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:62573"/>
            <dbReference type="ChEBI" id="CHEBI:62576"/>
            <dbReference type="EC" id="2.7.8.34"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the MobA family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.74" evidence="6"/>
    <dbReference type="EC" id="2.7.8.34" evidence="7"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P4Z5"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00135"/>
    <dbReference type="OrthoDB" id="15372at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000042">
        <property type="entry name" value="Bifunctional IPC transferase and DIPP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000282">
        <property type="entry name" value="Bifunctional IPC transferase and DIPP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053433">
        <property type="entry name" value="IPC_transferase/DIPP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041135">
        <property type="entry name" value="IPPtranDIPPsyn_Thcocales"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19136:SF84">
        <property type="entry name" value="BIFUNCTIONAL IPC TRANSFERASE AND DIPP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19136">
        <property type="entry name" value="MOLYBDENUM COFACTOR GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 18 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="260"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="384"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="MobA-like NTP transferase" evidence="20">
        <location>
            <begin position="5"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00018322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00054100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ASJ05852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="426" mass="47109" checksum="556A9D9B6FC0DF0F" modified="2017-09-27" version="1">MPEMAVILAAGLGTRMGGKPKGLVKVAGREILYRTMAILQRVGVKRFVLVTNDRYAPLYREFIEKYGFPAEVIINPEPEKGNGHSLHLAKGRVSGKFVLVMSDHVYSGVFLEKAIRGDGLIADRRPGWVDVGEATKVKVKNGRVEKIGKGLKEWDAVDTGFFVLDEGIFEVTEELERERNGDYSLSEVVERATLPVTFVDGLGWTDVDTPEDIKQARKMLVKTAVKGTGDGFVSRHLNRKISTEISALLVDKVTPNQMTAFTFALGIFSALLTLVSLPLAGILYQLSSILDGVDGEIARAQLRTSRLGGYIDSILDRYVDGTFLALLAYSTLSEPLWYLIALFALLGAVMVSYSTERFKGAFCRDAYAEVPSLRKLPGKRDERVFLTMLFLLYPIPVSIKALFAILAILTNLRVALTLYFVSRKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P515</accession>
    <name>A0A218P515_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853135.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P515"/>
    <dbReference type="GeneID" id="33314652"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00240"/>
    <dbReference type="OrthoDB" id="86161at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="123" mass="12809" checksum="FA475C5A692225DB" modified="2017-09-27" version="1">MNADERIPGALLYTGGIVLAFLRPPVDRIACITVPGGKVTSGINPFFLALEFGLVMVGSVLLALKHDFKNSHAKNGRISVASGLGVAFIGGYSGLRAVLLFGVMLATFGLVLYKVGGMRDADG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P516</accession>
    <name>A0A218P516_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Multidrug ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_00245</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853136.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P516"/>
    <dbReference type="GeneID" id="33314653"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00245"/>
    <dbReference type="OrthoDB" id="87732at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43613">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43613:SF1">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="4"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ05873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="248" mass="27510" checksum="A6B191AB3CFB9CD4" modified="2017-09-27" version="1">MPMVEVQNLEKDYGKVKALKGISFSINEGEIFGLIGPNGAGKSTTLKILATLLRPTGGKAEVSGYDVVEDAEEVRKLISYLPEEAGAYKNLTGLEYLSFMARLYAKDEEKAEDMLKLGIELSGLGERLRDKVATYSKGMTRKLLLARALMVRPKLAILDEPASGLDIVNAYEIRKTIKHFSREEGVTFLVSSHNMLEVEFLCDRVALINEGLIVESGTPRELKEKYDAENLEEVFMRVVGFEKRGGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P537</accession>
    <name>A0A218P537_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ACT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853166.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P537"/>
    <dbReference type="GeneID" id="33314685"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00405"/>
    <dbReference type="OrthoDB" id="85792at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006352">
        <property type="entry name" value="PRK08577.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="1">
        <location>
            <begin position="58"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="135" mass="14949" checksum="8F6291ED89E1F98C" modified="2017-09-27" version="1">MRHYEIVRIKENGKIEIPRDYAYDLGLVEGAYFLVEIDTDLKELHAERVALPGKRLVEVELVVEDRPGVLARISGLMGKHGANILFSESEELSSIGMAGIVAVIDVSGMEGTVEGLIEELKALPEVREVTFRSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P564</accession>
    <name>A0A218P564_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleotide-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853172.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P564"/>
    <dbReference type="GeneID" id="33314692"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00440"/>
    <dbReference type="OrthoDB" id="63170at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055552">
        <property type="entry name" value="DUF7128"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23447">
        <property type="entry name" value="DUF7128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="47" mass="5766" checksum="AD759326F0FA0BDB" modified="2017-09-27" version="1">MAELKAVVFYDRDGVRYYRCPRCGMLFRDSKEYTRHVNRSHGHLFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P578</accession>
    <name>A0A218P578_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853216.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P578"/>
    <dbReference type="GeneID" id="33314740"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00680"/>
    <dbReference type="OrthoDB" id="2837at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43122">
        <property type="entry name" value="FERREDOXIN SUBUNIT OF PYRUVATE:FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43122:SF1">
        <property type="entry name" value="IRON-SULFUR-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="65"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="167" mass="18481" checksum="E3390DB25B73C23E" modified="2017-09-27" version="1">MSEIPPYLQRGYITPEELFEIIPKPSEERLGARPVAVPECPQEIPCAPCREICPTGAISMPTPNDIPIVDYEKCIGCSLCVQICPGLAFFMVHYVGDKARITMPHELLPLPERGEEVVLLNRVGEPVGKGKVLTVVPREKTKGDTPIITVEVPIELAWDVRAVKVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P584</accession>
    <name>A0A218P584_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF356 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853183.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P584"/>
    <dbReference type="GeneID" id="33314704"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00500"/>
    <dbReference type="OrthoDB" id="73585at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007154">
        <property type="entry name" value="DUF356"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04009">
        <property type="entry name" value="DUF356"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006606">
        <property type="entry name" value="UCP006606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="16389" checksum="5074CDCE2A4A6482" modified="2017-09-27" version="1">MRNTIVLVRTDSFQKASIALADLVRYGGMKIRGDPRIIPPALSDWAFEQISGEKPRRKFRAHVVAQIDLPPAKAIGRLMDIHPPAHVLVIPPDTEVWEELMRLWKTFEKLKGFHPPKRTKAEELKRKREKKTDGEWELEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5B4</accession>
    <name>A0A218P5B4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">4-phosphopantoate--beta-alanine ligase</fullName>
            <ecNumber evidence="5">6.3.2.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Phosphopantothenate synthetase</fullName>
            <shortName evidence="5">PPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_00770</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the condensation of (R)-4-phosphopantoate and beta-alanine to 4'-phosphopantothenate in the CoA biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(R)-4-phosphopantoate + beta-alanine + ATP = (R)-4'-phosphopantothenate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27930"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="ChEBI" id="CHEBI:61294"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.2.36"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal phosphopantothenate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.36" evidence="5"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853230.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5B4"/>
    <dbReference type="GeneID" id="33314758"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00770"/>
    <dbReference type="OrthoDB" id="10078at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016881">
        <property type="term" value="F:acid-amino acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12640">
        <property type="entry name" value="Phosphopantoate/pantothenate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02224">
        <property type="entry name" value="PPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002855">
        <property type="entry name" value="PPS/PS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038138">
        <property type="entry name" value="PPS/PS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041123">
        <property type="entry name" value="phpantohe_syn_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010324">
        <property type="entry name" value="PRK13761.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40695">
        <property type="entry name" value="4-PHOSPHOPANTOATE--BETA-ALANINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40695:SF1">
        <property type="entry name" value="4-PHOSPHOPANTOATE--BETA-ALANINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02006">
        <property type="entry name" value="PPS_PS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004853">
        <property type="entry name" value="UCP004853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="181"/>
            <end position="183"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="187"/>
            <end position="188"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="199"/>
            <end position="200"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ05968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="262" mass="29660" checksum="71D5C3D961A5D1C0" modified="2017-09-27" version="1">MVKIPKSHPRYWSLYYREKIIEGMEKGMTAKAGLIAHGRGEAFDYIIGEKTIEPAERAMKAAVAKLLLAEHPVISVNGNVAALVPKETIELAKALNAKLEINLFYRTEERVKAIAEELRKYDPEIELLGINPTKRIPGLEHERGKVDENGIWKADVVVVPLEDGDRTEALVAMGKFVITIDLNPLSRSARMADITIVDNIVRAYPRMTELAREMKDYSREELLKIIEEYDNGKTLSDVLIHMRDRLTRLAEGGIWRKKELGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5B7</accession>
    <name>A0A218P5B7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADH oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_00660</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853213.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5B7"/>
    <dbReference type="GeneID" id="33314736"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00660"/>
    <dbReference type="OrthoDB" id="27922at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050260">
        <property type="entry name" value="FAD-bd_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429:SF3">
        <property type="entry name" value="NITRITE REDUCTASE [NAD(P)H]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="6">
        <location>
            <begin position="1"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="5">
        <location>
            <begin position="324"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ05951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="439" mass="47734" checksum="42EC78EB818AE902" modified="2017-09-27" version="1">MKIVVVGSGTAGSNFALFMRKLDRKAEITVIGKEPTMQYSPCALPHVISGTIEKPEDVIVFPNEFYEKQKINLMLNTEVKEIDCERKVVITDKGEVPYDKLVLAVGSKAFVPPIKGVENEGVFTLKSLDDVRRIKSYIAERKPKKAVVIGAGLIGLEGAEAFAKLGMEVLVVELMDRLMPTMLDKDTAKLVQAEMEKHGVSFRFGVGVSEIIGSPVRAVKISEEEVPADLVLVATGVRANIDLAKKAGLEVNRGIVVNEHLQTSDPDIYAIGDCAEVIDAVTGQRTLSQLGTSAVRMAKVAAEHIAGKDVSFRPVFNTAITELFGLEIGTFGITEERAKKEGIEIVTGKFKGSTKPEYYPGGKPITVKLIFRKSDRKLIGAQIVGGERVWGRIMTLSALAQKDATVEDVAYLETAYAPPISPTIDPITVAAEMAMRKFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5C3</accession>
    <name>A0A218P5C3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Proteasome-activating nucleotidase</fullName>
            <shortName evidence="9">PAN</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Proteasomal ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Proteasome regulatory ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Proteasome regulatory particle</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pan</name>
        <name evidence="12" type="ORF">A3L08_00835</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">ATPase which is responsible for recognizing, binding, unfolding and translocation of substrate proteins into the archaeal 20S proteasome core particle. Is essential for opening the gate of the 20S proteasome via an interaction with its C-terminus, thereby allowing substrate entry and access to the site of proteolysis. Thus, the C-termini of the proteasomal ATPase function like a 'key in a lock' to induce gate opening and therefore regulate proteolysis. Unfolding activity requires energy from ATP hydrolysis, whereas ATP binding alone promotes ATPase-20S proteasome association which triggers gate opening, and supports translocation of unfolded substrates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homohexamer. The hexameric complex has a two-ring architecture resembling a top hat that caps the 20S proteasome core at one or both ends. Upon ATP-binding, the C-terminus of PAN interacts with the alpha-rings of the proteasome core by binding to the intersubunit pockets.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">Consists of three main regions, an N-terminal coiled-coil domain that may assist in substrate recognition, an interdomain involved in PAN hexamerization, and a C-terminal ATPase domain of the AAA type.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853241.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5C3"/>
    <dbReference type="GeneID" id="33314771"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00835"/>
    <dbReference type="OrthoDB" id="77269at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022623">
        <property type="term" value="C:proteasome-activating nucleotidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000033">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000006">
        <property type="entry name" value="26S protease regulatory subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00553">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023501">
        <property type="entry name" value="Nucleotidase_PAN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003069">
        <property type="entry name" value="PRK03992.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01242">
        <property type="entry name" value="proteasome-activating nucleotidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 9 12" id="KW-0647">Proteasome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="172"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="18"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="183"/>
            <end position="188"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ05979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="398" mass="44996" checksum="8A238942DAC4CA33" modified="2017-09-27" version="1">MSVENIDVKPSDEYDDYITYLKRRIRQLELQVRTLEADKERLERELSRLRMEMSRLRQPPAFAGTLLEILDEDRAIVQNFNGPRFVVRIAPWIERDKLKPGSRVALDQRTMAVVELLPSEKDPSVLGFEVIERPQVTYTDVGGLEKQLQELREAIELPLKHPELFEKVGIEPPKGVLLYGPPGCGKTLMAKAVANHVNATFIRVVGSELVRKFIGEGARLVHELFELAKEKAPTIVFIDEIDAVGAKRMDETTGGEREVNRTLMQLLAEMDGFDPRGNVKVIAATNRPDILDPALLRPGRFDRLIEVPLPDFRGRLEILKVHTRKMNLKDVDLRIIAELTEGASGADLKAIATEAGMFAIRDRRTYVTQDDFLKAVEKVLGSEKRLAQAIAMHEVMYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5F5</accession>
    <name>A0A218P5F5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5F5"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01025"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="90" mass="10528" checksum="CE4CF09B430D3F05" modified="2017-09-27" version="1">MDFGLFMERYGYALLLRLMVILSFGVMVGTLLFWGYFTNGIAVIFGLLYAGIMILLKRLYEFLFGEDPNAISEKTEVDRERARSFFMNRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5G9</accession>
    <name>A0A218P5G9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Calcium-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853296.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5G9"/>
    <dbReference type="GeneID" id="33314832"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01140"/>
    <dbReference type="OrthoDB" id="102130at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="416" mass="44990" checksum="2616492890C756B3" modified="2017-09-27" version="1">MGKLTVLIVLLLLIGLYVYYTNPHIIDDLTRGVDDFRNGSPGSGGDSGRGTQTETQTGAGSGGSFGGTGWVANNTTGSPEVYIGDNFAAVPVNSSYILFADRNKDGKIDAVYMDTTGDGNYDTAYLDEDYNGKTDTWKTTFNGVSSYSWDVTGDGIPDVYDTNGDGKVDAWDLNSDGVIDERDVDYDGNPDLHDYNFDGVFDEFQTNVTFAPPESRNTSGTGAAGFSCPDNEEDAYRLYVQAYNNVTQLMAENQSSSDEAKRAYDIYLKARECYESFSSSTTSSSGPVTGTLGKIHRVTLDTDGAMAVKFSTGEVKASWTSGWEDVDLSAEPWCVDYPALLGHYVDIGEMSLDEVTINDIPSSGYPSSEVGEEVKAGHVYVNKNSDGTYTVFALVSHEKTGDCSHRITIAYRNVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5K5</accession>
    <name>A0A218P5K5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">TLDD-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_01185</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U62 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853302.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5K5"/>
    <dbReference type="GeneID" id="33314841"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01185"/>
    <dbReference type="OrthoDB" id="98233at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2290.10:FF:000003">
        <property type="entry name" value="Zinc-dependent protease, TldD/PmbA family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="entry name" value="PmbA/TldD superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051463">
        <property type="entry name" value="Peptidase_U62_metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025502">
        <property type="entry name" value="TldD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30624:SF0">
        <property type="entry name" value="METALLOPROTEASE SLR0863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30624">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN TLDD AND PMBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004919">
        <property type="entry name" value="TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="Putative modulator of DNA gyrase, PmbA/TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloprotease TldD/E N-terminal" evidence="5">
        <location>
            <begin position="20"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloprotease TldD/E central" evidence="7">
        <location>
            <begin position="106"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloprotease TldD/E C-terminal" evidence="6">
        <location>
            <begin position="220"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="453" mass="49342" checksum="F45A3F62D0829943" modified="2017-09-27" version="1">MEKLVRTAEELAGRYGIPYYEIRITRVTASHLSMGNGQLEELSLNTEVGIGVRAFNGAWGFSSANDIGRAEKAIETAMKIAKLSKGNSKIYTGDPIVDRAEMPVKKPFTDVEIEEKLALVKEASSLLTGEKIVSRSVYYGDGVKKQLYLNSLGSEIETVVPRIRFGFSVTAKENGEMQTYWKSFGGTTGWELVEGIDLPQWTEFVREKAISLLHAQAPPSGVFDVIMDPELTGVFIHEALGHAVEADSVKNGDSILAGRLGERIAVENLNVVDDPTLPGKFGSYIYDDEGIKAKRVELIKDGILVNYLNDRETSALLDLEPNGHGRAQGYAYQPLVRMGNTYVEPGDWSLDEMLGEVKNGLYMIGDKGGQVDTAGGTFTFGAKEGYIIENGEIKAQVRDVALSGKILEVLKNIRAIGKDTKIEFPGYCGKGQVVPVDDGGPHVLTRALVGGMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5M6</accession>
    <name>A0A218P5M6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">23S rRNA (Uracil-5-)-methyltransferase RumA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_01485</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853357.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5M6"/>
    <dbReference type="GeneID" id="33314902"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01485"/>
    <dbReference type="OrthoDB" id="85343at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030390">
        <property type="entry name" value="MeTrfase_TrmA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030391">
        <property type="entry name" value="MeTrfase_TrmA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048845">
        <property type="entry name" value="RUMT_ARLMC_TRAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010280">
        <property type="entry name" value="U5_MeTrfase_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00479">
        <property type="entry name" value="rumA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11061">
        <property type="entry name" value="RNA M5U METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11061:SF30">
        <property type="entry name" value="TRNA (URACIL(54)-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21579">
        <property type="entry name" value="PabTrmU54_TRAM_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05958">
        <property type="entry name" value="tRNA_U5-meth_tr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51687">
        <property type="entry name" value="SAM_MT_RNA_M5U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01230">
        <property type="entry name" value="TRMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01231">
        <property type="entry name" value="TRMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 7 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="tRNA (uracil(54)-C(5))/23S rRNA (uracil(747)-C(5))-methyltransferase TRAM" evidence="9">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="374"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="374"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="417" mass="47764" checksum="35969027513F7DBD" modified="2017-09-27" version="1">MRGIVERLDFDGMGIVKVRKKEIHVPFTVPGDVVEVKKWRRKKRRLVAVDFDVVEPSPERTEPRCPYFGTCGGCLLQHLPYEKQVEFKAEKLSALLSMDVEVVPSPVIYGHRNRIDVVISTKGIGFRRRGTWWDAVDIEECPVFGKTSRKVLRSLREFIGDHNPSLYEIGKNEGFLRYIVIREGKFTGELMVNLVTSEGALPEEFPGYFDYADSIYWSVNRTQSDVSYGEIERFWKSEFIRERLDDVTYLIHPNSFFQTNSYQAVTLVREVSRLVDGERVLDLYSGVGTFGIYLARRGFDVEGIEVNPFAVEMARRNVELNGVEAVFRVGEDRDVEDLSSYDTVVLDPPRAGLHPKLIRKILKDGPQSIVYVSCNPKTLVENLKVLYERYSVEDATGIDMFPHTPHVEAVVKLKLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5N6</accession>
    <name>A0A218P5N6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_01480</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853356.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5N6"/>
    <dbReference type="GeneID" id="33314901"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01480"/>
    <dbReference type="OrthoDB" id="6995at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053456">
        <property type="entry name" value="HTH-LrpA_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019885">
        <property type="entry name" value="Tscrpt_reg_HTH_AsnC-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040820">
        <property type="entry name" value="trans_reg_LrpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF7">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00519">
        <property type="entry name" value="HTH_ASNC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="2"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="141" mass="15868" checksum="34894766B3CF4FC7" modified="2017-09-27" version="1">MLDERDRIIIEMLTKDARTPFTEIAKVLGISETAVRKRVKALEESGVIKQYTVVVDPSKLGYNLVSLTGVDTLPEKIFEVANKLKEFEFVREVYLTSGDHMIMAEIWARDGEDLSDIISNKIGKIEGVTKVCPAIILEKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5P3</accession>
    <name>A0A218P5P3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0147 protein A3L08_01580</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_01580</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0147 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853373.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5P3"/>
    <dbReference type="GeneID" id="58919780"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01580"/>
    <dbReference type="OrthoDB" id="65304at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.50">
        <property type="entry name" value="Ta0600-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00342">
        <property type="entry name" value="UPF0147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023130">
        <property type="entry name" value="Ta0600-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005354">
        <property type="entry name" value="UPF0147"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003319">
        <property type="entry name" value="PRK04330.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03685">
        <property type="entry name" value="UPF0147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158436">
        <property type="entry name" value="Ta0600-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="83" mass="9287" checksum="F9DE8B33ED250AED" modified="2017-09-27" version="1">MSELIGQIVQVLKEQVVQDTVVPRNIRRAAEQAIEVLLDESKEPAVRAADAIAILEEISEDPNMPMHTRTIIWEVLGALEQVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5T8</accession>
    <name>A0A218P5T8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01650</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5T8"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01650"/>
    <dbReference type="OrthoDB" id="86142at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="351" mass="39329" checksum="3CB8BF11FC8B3E22" modified="2017-09-27" version="1">MLIQRGYYDEALAGIEDLEDPIEKVELLNDLAVAIHRKGGPEEWIPDIMEDAIYIAKKLKDPAHKATVYAIIAATLGILGYEDDAMDFFGRALDETDDIGSPIEKGIVLSTIAYHLTLCGHPESALDVFNVAFDTIIGAEVNYTHKVEGIVRIGELMEMAGDALPAEKALDFYRVAFDIFDKLHVNQRAAVVEKKIELAKTVYDVGLPEIRQSLLEGRYHYAIALIEKKYSGTARLIGELEVALWMRRINNIEYLDVVDKAFERCQNPVFTEANVQKIARLLTELGSLGRALKFAREIKDPRKRSEALKAIALELAKSQEFNEAREVIKDIPDPQVKAEALTEVATMEEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5W4</accession>
    <name>A0A218P5W4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Multidrug RND transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_01885</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family. MmpL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853428.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="GeneID" id="33314982"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01885"/>
    <dbReference type="OrthoDB" id="42357at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004869">
        <property type="entry name" value="MMPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050545">
        <property type="entry name" value="Mycobact_MmpL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33406">
        <property type="entry name" value="MEMBRANE PROTEIN MJ1562-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33406:SF6">
        <property type="entry name" value="MEMBRANE PROTEIN YDGH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03176">
        <property type="entry name" value="MMPL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="826"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="855"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="924"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="955"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1013"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1195"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1218"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1251"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1290"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1328"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="8">
        <location>
            <begin position="851"/>
            <end position="976"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="1372" mass="149317" checksum="1C316A4F282A8172" modified="2017-09-27" version="1">MAWNEWIAKHAKLVLLAWIVVIILMSPLAAKLSEVTNYSEEQMVSHNIESIKVQDIISEEFSGAQNENLTYLLITNVSVNDERAREAYYSFKERVEGKYADNVTSYYDALDGLWDMTYDLTLNITKMTANVTGLLYTTAIQTNEGFGQLLAAVYQLGNTTEMVKNGLVGAAQGYLALKQNVTGLYNQTVLLRNALNQTDLAYVALHRNLTSTAGMLKSLNSTLAELNQGLYTLNATYGKTYMGVIAVHRAVLASGAYERGSLTQAEAQAIASQTGTTPEFVYAVFNSVYPVYASAGSGAITDTFLANVTEGIVKAGSPAEQVPLVEAYGRAFYFGVRAFDEKVGSEYALQSMGPEELAQAVSGIATSALQSLPEVIAQSNQTVEVEGFGTIDAKTFSGIVETAISLGPNPSAEAVENATVEVALSFMGNVPNNPLSGLPNAKEVLKELLKTGPTKELERSILLSGMAENLPAEASQFAPVIVDAVLTYDENATRVLSKDPSVLEEATISVLKGITTQMGLSVDENLLKELYESNGSPEVLDKVALEMLKAGTAEQLSRAGVPNAEAIADVIVKTAAEDPQGIISGERLEEATVKVVSALSSGMGGNAEFDVSEIVKGLYEGADPRELAENLFLEGAREQMENANVSVPEEFRGVFLNLTETIVKNYPMGEEEIAKLVKGTVRSFVESYAKNNPYGIEITFDTSKLVDIAFEFKDRPDAMTMDDVKPLAEALFPTVLEKAGTYLSMLKSDDNTTMLITFVPRGRPGPSEDIYKYRAENAKRVKEIALEEFGKYFPEVDGALGGTPVQAHEMTEYGQRDNSVTTRASMIMALVVLFILMGMALLATFLPFTGVATSALTALGIAYLLAKGGIIDVGSWAQMLTITTALGLGIDYSTYYVHRFREYIAEGYEHEKAVAEALKRAKDAVLASAFTDIIAFASFVLAWEFPIFQQMGIIAPLAVVSVLIASLTFIPAITALIGDKSWFWWPRHIKHASALDVHERSKIAEWVVKHAKVVLIIALLIAAPATYTFVTFKGTHDMSLFLPEDSETLKFMTLSQEKLGASLMSPNYVIIEFSGNVSDDDLKLIDEITAHISTMEGVKAVYSPTRPYGEPVSNLTLSAVKALGGDRFISSRGDKVMIQVESSYRPTDEEAKNLVKALRAYVREVAEKNPRIKAGLVGGGAALSMDLTDRINDIFWHRIIPVALVLMFLSLIPTLKGLPAVASTMITIFLGVMTSIWVSTWLFERVFGQEVMWFLPLMVFVVLMGVGIDYNSFYLVKARDEFERRKPDEALIVAAGTMDKLVIGLAVVLASTYGALMLSSTWGTREMGFALAAGILLTATMAVYFIGPAFMSLFGEKAWWPLFKNRGEAGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5W9</accession>
    <name>A0A218P5W9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF3201 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01950</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853441.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5W9"/>
    <dbReference type="GeneID" id="33314995"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01950"/>
    <dbReference type="OrthoDB" id="85915at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024505">
        <property type="entry name" value="DUF3201"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11447">
        <property type="entry name" value="DUF3201"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142913">
        <property type="entry name" value="YktB/PF0168-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="172" mass="20105" checksum="39E27AEC5209BD7D" modified="2017-09-27" version="1">MNVREVHDFLNEMWEGIFTLNEELKAELPVEGFKVEDVEEVFGAYVFLDGEWRLMKYPHPAFEIKPQIEVGATPESYYFVVAVPKEKVSENFVGLFIEIFPRSFIYGAEDFLNDVYNWRRDGKVSPSEILEKISKSDEKVFQFEANFDSAKALKQGVKRLIELGKRFEIFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P600</accession>
    <name>A0A218P600_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Peptide ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_02155</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853478.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P600"/>
    <dbReference type="GeneID" id="33315036"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02155"/>
    <dbReference type="OrthoDB" id="312811at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050366">
        <property type="entry name" value="BP-dependent_transpt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43386:SF1">
        <property type="entry name" value="D,D-DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DDPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43386">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN APPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="249"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="284"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="344"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="389"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="214"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="415" mass="45737" checksum="7206B8FEEEA2D9F6" modified="2017-09-27" version="1">MGRKAGVVILVIFALFVVFSNAGVSDEDIANWENVNYWKDNPRMAYPSWFSVFGDRTPTSSLKPAVLGENGSVVYEFSYEHTYHDKPSDVRFYGLPYGREVEISVLRPDGIRVPLYKGIATSSNLSINTNMRTGVVSSLSDVLNLSEADYVLFSATELLFSRNGNMETLNGKYVFEVRLAGNATPSVEILGTCYGLLGTDSYGRDMWVGFVKGMNNTLYLAFFTTVIIVFLGAIIGILSGYVGGFLGEFMTFLLEVLVALPMLPILVVMVWLFSTQGYGEQVRINSVLFMFFVALLTLGKFAKTVRTMTIREKVNEYVSAAVSMGAGTLWVLRKHILPPVGEFSLMYSTILLARIVALISVFGFFGLIPGTNWGSFMIEAMNQGALYGGYWWWIVAPGFAMAVLSAGLALFSSRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P611</accession>
    <name>A0A218P611_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_02160</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853479.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P611"/>
    <dbReference type="GeneID" id="33315037"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02160"/>
    <dbReference type="OrthoDB" id="372125at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050755">
        <property type="entry name" value="TRAFAC_YlqF/YawG_RiboMat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089">
        <property type="entry name" value="GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089:SF30">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN-LIKE 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="3">
        <location>
            <begin position="7"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="357" mass="40921" checksum="33E501A1B0CB0EC0" modified="2017-09-27" version="1">MKARKAWRVVREVIDEADVVVEVVDARDPIGTRNRKLERLIMEEGKPLLIVMNKADLVPKEWAEEYKRKSEIPVVFISARERKGTGILRNEIKKLAKPLLEEKERVKVALIGYPNVGKSTIINTLKGRRAVGTAPIPGYTKGKQLIRLSKRIWLLDSPGVIPIDDFDELVIKGGFPADKIEEPVKPALKLISRILETRKEALTEKFEIEDFESEEEILRKIGERRGLIKTGGEVDLEETARWFLREWQTGRFTLFGKGEERPQEFKWDFEDILEGIEKDLLLDPRRILWKYGDELREKLDNQKRVGVREIEGFAVGIATGFKKCGPAVKLLEELTGKHVLASECFGKKWKGVVVVME</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P655</accession>
    <name>A0A218P655_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UPF0056 membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_02440</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the UPF0056 (MarC) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853528.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P655"/>
    <dbReference type="GeneID" id="33315093"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02440"/>
    <dbReference type="OrthoDB" id="86226at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002771">
        <property type="entry name" value="Multi_antbiot-R_MarC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33508">
        <property type="entry name" value="UPF0056 MEMBRANE PROTEIN YHCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33508:SF1">
        <property type="entry name" value="UPF0056 MEMBRANE PROTEIN YHCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01914">
        <property type="entry name" value="MarC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="204" mass="22026" checksum="189EBBB70845D615" modified="2017-09-27" version="1">MSEVLSILSSALFMLIMIDPSDKILLVSLLREDFHIEDIRTLIVRANLIGFLLLFLFAISGQIILQEIFHIDINALRVAGGFVLFKIGLEALESGGMMTLKKEKNILALAAVPVATPLIAGPAAITTAITLTAEKGLYHATAAILLAIIFTALTMFITLYLIKNVSKTTLGVFIRIIGMFTMAIGAQMMVQGVIGIYLLMTAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P674</accession>
    <name>A0A218P674_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ABC transmembrane type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_02625</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853564.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P674"/>
    <dbReference type="GeneID" id="33315131"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02625"/>
    <dbReference type="OrthoDB" id="44105at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30465">
        <property type="entry name" value="INNER MEMBRANE ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30465:SF0">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN APPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="120"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="85"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="321" mass="36235" checksum="94E951F4E2A2FBF7" modified="2017-09-27" version="1">MAKAGAVAKIITRNLLILFLALLVVGIVIGGGELRIERNDLGKVYKFYPKNDSSLLDIVGGYFSATWKFLTDPPEVWEEGLETFVVKSFALLVLTEVFLLVIGLFLGLRAGYYRGLADKVVSALAPTFSAMPSWFIGVVFLFLFYWKLSLFPVNFEDTINWAEVHGTLSTSTYLRALVLPVLTLVFSGLWEYAFNVRNMIVNERNGDHVLYDVARGLPEGRIMRKLLRTALPAFLTFTTYNFLEILTGMMLIEVMFNIHGLGYLMAVSFWLTRIGDGFGFAYAPEILFFTTAVMMLFYFINAVVMEGLYLYLDPRSGGERQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P687</accession>
    <name>A0A218P687_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">KaiC-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_02410</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853523.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P687"/>
    <dbReference type="GeneID" id="33315087"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02410"/>
    <dbReference type="OrthoDB" id="100639at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11570">
        <property type="entry name" value="Protein of unknown function DUF257"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005489">
        <property type="entry name" value="DUF257"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03192">
        <property type="entry name" value="DUF257"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="24729" checksum="57C079C1C935FBBB" modified="2017-09-27" version="1">MGLRDSVMFEIWESIKMGETVLFERVGEGDMTFGFYQLLDWAEKKGFRTIIVDVLDSYPAMVSKMKLMGIETEKLSELEVIKIGGLRKRGKIIAHIENTSEPTVLVRKFREAYQPPIENPSGKVLAIIVGLEKLFAISDLTIRGVQTIVSYLASYVGMPNRLGIYFLKRDVLSGDKELSLKLLEDIATTVIRAEKKGHMIKLHIAKSLNKELEGVLLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P698</accession>
    <name>A0A218P698_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">A-type ATP synthase subunit A</fullName>
            <ecNumber evidence="13">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">atpA</name>
        <name evidence="15" type="ORF">A3L08_02600</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane. The A chain is the catalytic subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal alpha chain is a catalytic subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="13"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853559.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P698"/>
    <dbReference type="GeneID" id="33315126"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02600"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18111">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18119">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01134">
        <property type="entry name" value="V_A-ATPase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000675">
        <property type="entry name" value="V-type ATP synthase alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1140.10:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000008">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00309">
        <property type="entry name" value="ATP_synth_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031686">
        <property type="entry name" value="ATP-synth_a_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005726">
        <property type="entry name" value="ATP_synth_asu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022878">
        <property type="entry name" value="V-ATPase_asu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01043">
        <property type="entry name" value="ATP_syn_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003220">
        <property type="entry name" value="PRK04192.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607:SF1">
        <property type="entry name" value="H(+)-TRANSPORTING TWO-SECTOR ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607">
        <property type="entry name" value="V-TYPE PROTON ATPASE CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16886">
        <property type="entry name" value="ATP-synt_ab_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 13" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 13" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="14">
        <location>
            <begin position="223"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="231"/>
            <end position="238"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ASJ06297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="585" mass="65362" checksum="026F9D2AE04970CB" modified="2017-09-27" version="1">MGRIIRVTGPLVVADGMKGSKMYEVVRVGEMGLIGEIIRLEGDRAVIQVYEETAGIKPGEPVEGTGASLSVELGPGLLTAMYDGIQRPLNVLRDLSGDFIARGLTAPALPRDKKWHFTPKVKVGDKVVGGDILGVVPETSIIEHRILVPPWVEGEIVEIAEEGDYTIEEVIAKVKKPDGSIEELKMYHRWPVRVKRPYKNKLPPEVPLITGQRTIDTFFSQAKGGTAAIPGPFGSGKTVTQHQLAKWSDAQVVVYIGCGERGNEMTDVLEEFPKLKDPKTGKPLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDQGYDVALMADSTSRWAEALREISGRLEEMPGEEGYPAYLASKIAEFYERAGRVITLGSDERIGSVSVIGAVSPPGGDFSEPVVQNTLRVVKVFWALDADLARRRHFPAINWLRSYSLYVDAIQGWWEQNVDPEWRKMRDQAMALLQKEAELQEIVRIVGPDALPDREKAILIVTRMLREDYLQQDAFDEVDTYCPPKKQVTMMRVILNFYEKTMEAVDRGVPVDEIAKLPVREKIGRMKYEPELENVKALIDETNAQFEELFKKYGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6A3</accession>
    <name>A0A218P6A3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02635</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6A3"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02635"/>
    <dbReference type="OrthoDB" id="99172at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="214" mass="24190" checksum="5630B90FD6C26D15" modified="2017-09-27" version="1">MLWELLSLVLLLIFLGINVMSWFIRIGGKLAGVTLRKALRVDVPRNEKKGLERLFTLIWVAIGLWASFKVRWNYLAMLFAFLAFRSGANITRTLVYSIHDGKVLEKYAGDSRALSLLSRAVRASLLLEGLFVFAFGLAYKAISMTARPGEFSRGLFILELWLAGLVFGALFGWLIARNNRGILLENQIVVVYFFAGKKGREKVNEFGRKVKLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6D9</accession>
    <name>A0A218P6D9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribonuclease P protein component 2</fullName>
            <shortName evidence="6">RNase P component 2</shortName>
            <ecNumber evidence="6">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Pop5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rnp2</name>
        <name evidence="7" type="ORF">A3L08_02840</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eukaryotic/archaeal RNase P protein component 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853606.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6D9"/>
    <dbReference type="GeneID" id="33315174"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02840"/>
    <dbReference type="OrthoDB" id="19261at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3250">
        <property type="entry name" value="Ribonuclease P, Pop5 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00755">
        <property type="entry name" value="RNase_P_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002759">
        <property type="entry name" value="Pop5/Rpp14/Rnp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038085">
        <property type="entry name" value="Rnp2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016434">
        <property type="entry name" value="Rnp2_archaea"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002986">
        <property type="entry name" value="PRK03717.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15441">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15441:SF2">
        <property type="entry name" value="RIBONUCLEASE P_MRP PROTEIN SUBUNIT POP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01900">
        <property type="entry name" value="RNase_P_Rpp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004952">
        <property type="entry name" value="RNase_P_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160350">
        <property type="entry name" value="Rnp2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="120" mass="13910" checksum="6289995AC59F37D2" modified="2017-09-27" version="1">MREKPKYLPPTLRDKHRYIAFQVIGERAFTKDEIKRAIWDASLSTLGVLGSAKAKPWFIKFDEKSQTGIVRVDRKHVEELRLALTLVTHVNGSRAIFRTLGVSGTIKRLKRKFLAEYGWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6E0</accession>
    <name>A0A218P6E0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Isoleucine--tRNA ligase</fullName>
            <ecNumber evidence="10">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Isoleucyl-tRNA synthetase</fullName>
            <shortName evidence="10">IleRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ileS</name>
        <name evidence="14" type="ORF">A3L08_02875</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>tRNA(Ile) + L-isoleucine + ATP = L-isoleucyl-tRNA(Ile) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:9666"/>
            <dbReference type="Rhea" id="RHEA-COMP:9695"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="10"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853610.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6E0"/>
    <dbReference type="GeneID" id="33315181"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02875"/>
    <dbReference type="OrthoDB" id="30823at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07961">
        <property type="entry name" value="Anticodon_Ia_Ile_ABEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00818">
        <property type="entry name" value="IleRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000325">
        <property type="entry name" value="Isoleucine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000033">
        <property type="entry name" value="Valine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02003">
        <property type="entry name" value="Ile_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033709">
        <property type="entry name" value="Anticodon_Ile_ABEc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023586">
        <property type="entry name" value="Ile-tRNA-ligase_type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780:SF1">
        <property type="entry name" value="ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780">
        <property type="entry name" value="SOLEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19302">
        <property type="entry name" value="DUF5915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 10 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="12">
        <location>
            <begin position="20"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="13">
        <location>
            <begin position="711"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="10">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="10">
        <location>
            <begin position="625"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="628"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ06348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="1067" mass="125330" checksum="6E52180EE27FB797" modified="2017-09-27" version="1">MIKEPEFREYSPGKLEEKIERFWEENNTYEKVKASRANGPKYYFLDGPPYVSGAIHLGTAWNKIIKDMVIRFRSMQGYNVRRQPGFDMHGLPIEVKVEQALGLKVKKDIETEIGVDNFIKKCKEFALNNLKIMTEQFKQLGIWMDWDDPYMTIKNEYIESGWFTLKRAWEKGLLEKDKRVLHWCPRCETALAEHEVRGEYKIRKDPSIYVKFPIEGKENEYLLIWTTTPWTLPANLAVTVHPEYEYARVRVETEKGEEYWIIAKALVERVLSEVGVKGEIVEEFKGEELEGIRYVHVLMDEYPAQKEFREKYEWAHRVILGEHVTLEDGTGLVHTAPGHGEEDFEVGQKYGLPVYSPVDDEGRYTEGFWKGTYVKDADREIIEHLTEKGYLVKAGEIEHKYPHCWRCKTPLIFRATDQWFLKVSRVKDKIIKENDEKVTWYPDWVKVRYDNGVMNSGDWVISRQRYWGIPLPIWQSEDGEIYVVGSFKELVEKSVAIEVNGERIELPEDYNEKLKIIEEKLGPEDLHRPYVDAFIIKVNGKEMRRVKDVVDVWFDSGIASWASLGYPRNKELFEKLWPADFIVEGEDQVTKWFYSQQAASVIAFDTVPYRKVAMHGYVLDEKGDKMSKSLGNIIRPEEVVQKEGRDPFRFYMLWATNPWENLRFSWKGLEQVKRMLNILWNVYVLSATYMSLDNFDPTKLKPEELPFREEDKWILSRVNSLIGEVTEGIETFRLTRATRAIYNFVVEDLSRWYVRLIRKRMWVEGDDPDKLTAYYTVWKVFDVLLRLMAPFTPYIAEEIYQNLMRPFLGVESVHMLDWPKADEKAVDEELEREMDVVRKIVEAGSSARQRAKIKLRYPVRRIIIETEDETVKKAVERLNRLLKDQLNAKEVAVGKVERELIIKPNFAKVGPEFKGDARLVTKWISEHGRELYEKGEMDVEIEGKTFHLTREHLTVEEKLPDFLVAEEFEGGKVFIDKTLTRELLAEGLAREFVRRIQEMRKRLDLDVNDRIKVTIETTDENRELLSENLDYITGETRAVEVIFGEAKGYVVEWPEVEAKIGIEKVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6E2</accession>
    <name>A0A218P6E2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Probable inosine/xanthosine triphosphatase</fullName>
            <shortName evidence="10">ITPase/XTPase</shortName>
            <ecNumber evidence="10">3.6.1.73</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Non-canonical purine NTP phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Non-standard purine NTP phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Nucleoside-triphosphate phosphatase</fullName>
            <shortName evidence="10">NTPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">yjjX</name>
        <name evidence="12" type="ORF">A3L08_02880</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Phosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP to their respective diphosphate derivatives. Probably excludes non-canonical purines from DNA/RNA precursor pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>ITP + H2O = IDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28330"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58280"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.73"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>XTP + H2O = XDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28406"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59884"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.73"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="10">Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn(2+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the YjjX NTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.73" evidence="10"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853611.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6E2"/>
    <dbReference type="GeneID" id="33315182"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02880"/>
    <dbReference type="OrthoDB" id="52857at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103023">
        <property type="term" value="F:ITPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006772">
        <property type="term" value="P:thiamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.950.10:FF:000002">
        <property type="entry name" value="Inosine/xanthosine triphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00648">
        <property type="entry name" value="Non_canon_purine_NTPase_YjjX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002786">
        <property type="entry name" value="Non_canon_purine_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026533">
        <property type="entry name" value="NTPase/PRRC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050299">
        <property type="entry name" value="YjjX_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00258">
        <property type="entry name" value="inosine/xanthosine triphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003039">
        <property type="entry name" value="PRK03941.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34699">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34699:SF2">
        <property type="entry name" value="NON-CANONICAL PURINE NTP PHOSPHATASE_PRRC1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01931">
        <property type="entry name" value="NTPase_I-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 10" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Non-canonical purine NTP phosphatase/PRRC1" evidence="11">
        <location>
            <begin position="6"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="7"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="193" mass="20905" checksum="3DF98D104FDF037C" modified="2017-09-27" version="1">MRIAVGSTNPTKVLAVKEVMEMIYGDVEVFGVEVESGVPDQPVGIEETIRGAINRARQALEKGNADMGVGIEAGIYPVPNSLTGYFDIQFCAILDREGWLTIGHGPGFEYPPYVIKRIKEGVEAGKAMDELVGEKDLKRKTGAIGYLTHGILPRKELNKLAVLMAMVPRMNPELFGLKDVPSPTAPRSPLTGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6E6</accession>
    <name>A0A218P6E6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CCA-adding enzyme</fullName>
            <ecNumber evidence="10">2.7.7.72</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">CCA tRNA nucleotidyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA CCA-pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA adenylyl-/cytidylyl- transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA nucleotidyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA-NT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cca</name>
        <name evidence="14" type="ORF">A3L08_02920</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. tRNA 3'-terminal CCA addition is required both for tRNA processing and repair. Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs. While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14433"/>
            <dbReference type="Rhea" id="RHEA-COMP:10465"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:74896"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="EC" id="2.7.7.72"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a tRNA with a 3' CCA end + 2 CTP + ATP = a tRNA with a 3' CCACCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:76235"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="Rhea" id="RHEA-COMP:18655"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="ChEBI" id="CHEBI:195187"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="10">A single active site specifically recognizes both ATP and CTP and is responsible for their addition.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.72" evidence="10"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853619.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6E6"/>
    <dbReference type="GeneID" id="33315190"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02920"/>
    <dbReference type="OrthoDB" id="7378at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:CCA tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160016">
        <property type="term" value="F:CCACCA tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042245">
        <property type="term" value="P:RNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001680">
        <property type="term" value="P:tRNA 3'-terminal CCA addition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05400">
        <property type="entry name" value="NT_2-5OAS_ClassI-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1550">
        <property type="entry name" value="Archaeal tRNA CCA-adding enzyme catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.30">
        <property type="entry name" value="CCA tRNA nucleotidyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.590">
        <property type="entry name" value="Poly(A) polymerase predicted RNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01264">
        <property type="entry name" value="CCA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048833">
        <property type="entry name" value="CAA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008229">
        <property type="entry name" value="CCA-adding_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042090">
        <property type="entry name" value="CCA_tRNA_nucleotrans_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006116">
        <property type="entry name" value="NT_2-5OAS_ClassI-CCAase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011068">
        <property type="entry name" value="NuclTrfase_I-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015329">
        <property type="entry name" value="tRNA_NucTransf2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03671">
        <property type="entry name" value="cca_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39643">
        <property type="entry name" value="CCA-ADDING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39643:SF1">
        <property type="entry name" value="CCA-ADDING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21133">
        <property type="entry name" value="CAA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09249">
        <property type="entry name" value="tRNA_NucTransf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005335">
        <property type="entry name" value="CCA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55003">
        <property type="entry name" value="PAP/Archaeal CCA-adding enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0692">RNA repair</keyword>
    <keyword evidence="9 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="11">
        <location>
            <begin position="37"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA nucleotidyltransferase substrate binding" evidence="12">
        <location>
            <begin position="155"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="CCA-adding enzyme C-terminal" evidence="13">
        <location>
            <begin position="294"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF09249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF21133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ06357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="454" mass="53039" checksum="92D750C37D2B56AF" modified="2017-09-27" version="1">MDAESVIKEVLQQIRPTEEERAFIENLMRELEELAVETIEELGLDVKPYFVGSLAKDTYLAGDHDVDLFLAFPLETPLEELREKGLELGKAMAERLDSYEIAYAEHPYVRARYRGVSVDLVPCYDVKSWKDVRTAVDRSILHTEWVNENIRGKNDEVRLLKRFLKGINAYGSEIYVRGFSGYLAEILVIKYGSFLEVLEKADFILRQKIIDPGNWLKREYETAMNTVKREADEDRPLIVIDPVDPRRNVAANLGWERYGIFYFKAMKFLEEPSTKFFFPEKPRAGNYLDELRRKGTHLVVLAFEKPDLVDDLLLPQLERSARGFEKALEREGFRVFGWDYGYSGDKAFIMLELDRVERERVTIKPGPEFFTERGLDFYRKNERVWVRGKRLYAEKRVKENIVDVISELLEKNQVALGKGIREMIKLADVLADYAPKELEQSAYLFLSREKWNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6H3</accession>
    <name>A0A218P6H3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_03125</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6H3"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03125"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="164" mass="17803" checksum="9EEA0E3A5D6DDE04" modified="2017-09-27" version="1">MSLKRRNRDWGLAVYFLYGVLVLNAASFSDFLSYQALQVVLMLVLPSLMALREMMVGFEIPGKVEPEVLIPAAPFILGAVYDVLFFAGIVLTLLSGFKSQHLVTAKNLLVPVGFLTVVIAIIRGGPISSPHPRDPDRDDGCGGDIRHPARTGREGGGRVQGRVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6I9</accession>
    <name>A0A218P6I9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853642.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6I9"/>
    <dbReference type="GeneID" id="33315216"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03050"/>
    <dbReference type="OrthoDB" id="92051at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="62" mass="7275" checksum="D1D62F70842B24C1" modified="2017-09-27" version="1">MGKIAIELNVPDEILKTIDPKRLKRIVEREVIAEYSAQKLHGKFRGMDLKKLLREVEEEWGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6J4</accession>
    <name>A0A218P6J4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">AAA family ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_03180</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853662.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6J4"/>
    <dbReference type="GeneID" id="33315242"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03180"/>
    <dbReference type="OrthoDB" id="45637at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.270:FF:000007">
        <property type="entry name" value="DNA helicase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000326">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050534">
        <property type="entry name" value="Coronavir_polyprotein_1ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004483">
        <property type="entry name" value="SMUBP-2/Hcs1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00376">
        <property type="entry name" value="IGHMBP2 family helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788:SF8">
        <property type="entry name" value="DNA-BINDING PROTEIN SMUBP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="6">
        <location>
            <begin position="175"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="7">
        <location>
            <begin position="429"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="660" mass="75041" checksum="585F32416CBAD266" modified="2017-09-27" version="1">MSENETLMKFVSRLKELVELERKAEIEAMRLEMKRLSGREREKVGRAILGLNGKIVGEELGYFLVRYGRDREIKTEISVGDLVVISKRDPLKSDLVGTVVEKGKRFITVALETVPEWALKGVRVDLYANDITFKRWIENLDNLRDSGRKALELYLGLREPEAGEPVSFSPFDRSLNASQKEAVSRALSSPDFFLIHGPFGTGKTRTLVELIRQEVERGNRVLATAESNVAVDNLVERLAGSGIKVVRVGHPSRVSKNLHETTLAYLITQHELYGELRELWLTGQALAEKRDTYTKPLPKYRRGLTDLEILALSRRRRGTRGVPAKLIREMANWIKLNKLAQKAFDDARKLEERIAREIIENADVVLTTNSSAGLDVVNYAEYDVAVIDEATQATIPSVLIPINRAKRFVLAGDHKQLPPTILSEKAKELSKTLFEGLIERHPNKSAMLTVQYRMNERLMEFPSREFYDGRIIADESVRCITLADLGVKSPAFGNSWDEVLEPRNVLVFIDTSKRDDRFERQRRGSDSRENPLEAKLVKETVEKLLRIGVKPEWIGVITPYDDQRDLISSLLPEEIEVKTVDGYQGREKEVIVLSFVRSNERGEIGFLKDLRRLNVSLTRAKRKLILIGDSSTLSAHPTYRRLFEFVRERETVIGANELGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6L6</accession>
    <name>A0A218P6L6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1464 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853660.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6L6"/>
    <dbReference type="GeneID" id="33315240"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03170"/>
    <dbReference type="OrthoDB" id="146520at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009927">
        <property type="entry name" value="DUF1464"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07318">
        <property type="entry name" value="DUF1464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009433">
        <property type="entry name" value="DUF1464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="355" mass="39294" checksum="1489ADF27FEECE9B" modified="2017-09-27" version="1">MRVIGVDPGTKSFDVIGLEDGKIKLDLSFPSEVVAEEPGKIVKAIEEFNADLIIGPSGYGVPLKHISELTERDRFEMTLVREDELKEIPVLIGLQKMVSEMAEKGMNVWFIPGVIHLPTVPEWRKYNKIDMGTADKMAITVLGIYDQAKRLGIEYKDVSFVLLEVGFGYNYAGAVKGGKIVDGIGGTIFPGPAYVNSGALDGEVAYLLREIKKWHLFWGGATVIAANEILPPEEFARRLDEEPFARAWEAMKDGFLKAVASELAVVREAKEIILSGRLMRIDELREDVEDLFEEHFGLPMVRQRGLEGMAKEAAQGSAIIADGLAGGEFKELVEHVEIKKSRGSVLDYVKFPLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6P3</accession>
    <name>A0A218P6P3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_03465</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853711.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6P3"/>
    <dbReference type="GeneID" id="33315298"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03465"/>
    <dbReference type="OrthoDB" id="39312at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07577">
        <property type="entry name" value="Ph0642_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050345">
        <property type="entry name" value="Aliph_Amidase/BUP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053613">
        <property type="entry name" value="Aliphatic_Nitrilase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040852">
        <property type="entry name" value="nitrile_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43674:SF2">
        <property type="entry name" value="BETA-UREIDOPROPIONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43674">
        <property type="entry name" value="NITRILASE C965.09-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CN hydrolase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="264" mass="29603" checksum="AFA1DE91F829C456" modified="2017-09-27" version="1">MKVAYVQMRPVFLEPEVNYSRAEELIRIAADKGAKLIVLPELFDTGYNFRSREEVESVAGEIPDGPTTQFLMELSRELGVFIVAGTAEKDENGKLYNSAVITGPIGSGYIGKYRKVHLFHREKLFFEPGNLGFHVFNIGIAKVGVMICFDWFFPESARTLALKGADIIAHPSNLVMPYAPRAMPIRALENRVYTITANRVGEEFGLRFIGKSTIASPKAEVLAMGSEDGEEVGVVEIDLELARNKRINEMNDVFRDRRPEHYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6P8</accession>
    <name>A0A218P6P8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MFS transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853706.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6P8"/>
    <dbReference type="GeneID" id="33315292"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03430"/>
    <dbReference type="OrthoDB" id="98755at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="87" mass="9866" checksum="407019DFB40299B7" modified="2017-09-27" version="1">MDFALFMERYGYRLLMLITVSVILGIALAPFIMTFWAFSSDGLAVAVLAVIILGIGVLLMAIPKFWDFADKMRHTHILEDWNEKGND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6S3</accession>
    <name>A0A218P6S3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">2-(3-amino-3-carboxypropyl)histidine synthase</fullName>
            <ecNumber evidence="3 10">2.5.1.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_03505</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the first step of diphthamide biosynthesis, i.e. the transfer of the 3-amino-3-carboxypropyl group from S-adenosyl-L-methionine (SAM) to the C2 position of the imidazole ring of the target histidine residue in translation elongation factor 2 (EF-2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-histidyl-[translation elongation factor 2] + S-adenosyl-L-methionine = 2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36783"/>
            <dbReference type="Rhea" id="RHEA-COMP:9748"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="EC" id="2.5.1.108"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DPH1/DPH2 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.108" evidence="3 10"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853718.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6S3"/>
    <dbReference type="GeneID" id="33315306"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03505"/>
    <dbReference type="OrthoDB" id="314at2157"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11850:FF:000004">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11860:FF:000003">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11840:FF:000003">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11850">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042264">
        <property type="entry name" value="DPH1/DPH2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035435">
        <property type="entry name" value="DPH1/DPH2_euk_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022428">
        <property type="entry name" value="Dph2_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03682">
        <property type="entry name" value="arCOG04112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762:SF1">
        <property type="entry name" value="2-(3-AMINO-3-CARBOXYPROPYL)HISTIDINE SYNTHASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004967">
        <property type="entry name" value="DPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01121">
        <property type="entry name" value="Diphthamide_biosynthesis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 10 11" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR004967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="342" mass="38116" checksum="079423BA360ACB64" modified="2017-09-27" version="1">MHEIPFEEILQRLLDLKANRVLIQTPEGLKGEAQALADFLERNGIEAIISGDINYGACDPADTEAKRLGCGALIHLGHSYMALHLEVPTLFVPAFAKVDVVPALERNIEEIKKLGKKIALVTTTQHIHQLERARTFLEENGFEVLVGKGDSRVSWPGQVLGCNFSAARVNADGVLFIGAGYFHPIGLAISTRKPTLAVNPYSGDAIWMDKEAERLIRKRWAQIAKAMDAERFGVITSTKKGQLRLAEAKRVVKLLREHGKYAKLIAMNHISYPALEGFDFDAYVVVACPRVPIDDYENWRKPVLTPREVEILLGLRDDYEFDEILGVERKEDEPPGISVKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6T2</accession>
    <name>A0A218P6T2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Translation initiation factor 2 subunit gamma</fullName>
            <ecNumber evidence="11">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">aIF2-gamma</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">eIF-2-gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">eif2g</name>
        <name evidence="13" type="ORF">A3L08_03570</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EIF2G subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853728.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6T2"/>
    <dbReference type="GeneID" id="33315319"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03570"/>
    <dbReference type="OrthoDB" id="7798at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01888">
        <property type="entry name" value="eIF2_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03688">
        <property type="entry name" value="eIF2_gamma_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15490">
        <property type="entry name" value="eIF2_gamma_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000009">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000075">
        <property type="entry name" value="Translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00119">
        <property type="entry name" value="eIF_2_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050543">
        <property type="entry name" value="eIF2G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015256">
        <property type="entry name" value="eIF2g_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044127">
        <property type="entry name" value="eIF2g_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044128">
        <property type="entry name" value="eIF2g_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022424">
        <property type="entry name" value="TIF2_gsu"/>
    </dbReference>
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        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03680">
        <property type="entry name" value="eif2g_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003077">
        <property type="entry name" value="PRK04000.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09173">
        <property type="entry name" value="eIF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 11 13" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tr-type G" evidence="12">
        <location>
            <begin position="9"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="21"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
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    <feature type="binding site" evidence="11">
        <location>
            <position position="48"/>
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        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="145"/>
            <end position="148"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="180"/>
            <end position="182"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="410" mass="44790" checksum="F46B4ED2D188E21B" modified="2017-09-27" version="1">MAKKKEFRQAEVNIGMVGHVDHGKTTLTKALTGIWTDTHSEELRRGITIKIGFADAEIRKCPNCGRYSSSPVCPYCGHETKFERRVSFIDAPGHEALMTTMLAGASLMDGAVLVIAANEGIMPQTREHLMALQIVGNKNIVIALNKIELVDRETVMKRYEEVKEFVKGTVAENAPIIPISALHGANVDVLLAAIEEFIPTPKHDLRKPPKMLVLRSFDVNKPGTPPEKLVGGVIGGSIVQGKLKVGDEIEIRPGVPYEEHGRIRYEPITTEIVSLQAGGKFVDEAYPGGLVGVGTKLDPFLTKGDLMAGNVVGKPGKLPPVWDDITLEVHLLDRVVGTEEELKVEPIKRREVLLLNVGTARTMGLVTGLGKDTVELKLQIPVCAEPGDRVAISRQVGSRWRLIGYGFIRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6U9</accession>
    <name>A0A218P6U9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Multidrug transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_03755</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853761.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6U9"/>
    <dbReference type="GeneID" id="33315356"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03755"/>
    <dbReference type="OrthoDB" id="5596at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd04487">
        <property type="entry name" value="RecJ_OBF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04473">
        <property type="entry name" value="S1_RecJ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
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        <property type="entry name" value="DHH_sf"/>
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    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
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        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
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        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
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        <property type="entry name" value="S1_domain"/>
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        <property type="entry name" value="SSDNA_exonuclease_RecJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30255">
        <property type="entry name" value="SINGLE-STRANDED-DNA-SPECIFIC EXONUCLEASE RECJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30255:SF2">
        <property type="entry name" value="SINGLE-STRANDED-DNA-SPECIFIC EXONUCLEASE RECJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CR-type" evidence="3">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="2">
        <location>
            <begin position="133"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="740" mass="82770" checksum="A4D970382008830A" modified="2017-09-27" version="1">MVVKECPECRGTGKVKVGEKECPVCEGWGYVPADFNVGDKLKGYRNLDYLGVEDEVDEIPCPECHGKGVVPVYDTCPTCGGTGRVLACDICGKIKEPWEPGMETTWVCPDCLRKHKVVYILDKTCDYEDVEVGSVYKGTIDRVERFGVFVRLNPHVTGLIKRKDLLGGREYKPGEEILVQVLDVRPDKKEVDLVESALRNYKEVVVRKEIPVTPIRELSKDMAGKTVRLRGRVTQIQVTGGPTVFTITDGTGITWVAAFEAPGVRAYPNINVGDVVEVIGKVAFHAGEIQIEASDMTRLWGPDAAQVKQAIERALDERAQPKDVGFLVESEVLEKLKPKIMKAAFMIRRAILEGRPILLRHHSDADGYTSGLALEYAIVPLIEQVSPDSGARWKLFKRRPSRAPFYELEDVLKDIIFMIEDHEKFGDPLPLVVIVDNGGTSEDIPAYKRIRAYGVPIVVIDHHDPREWISEDKAKVDEYVDVHVNPHHVKRGYYELTAGMLATEVARFINPEVEDKIKHLPAIAGTGDRSKAPEFQQYLEIAKKTKGLTEEDLKKIAEVIDHEAFYWKFMDGHGIIDEILLLTGNLQRHRELINAIYPEVKEKQEKALRASLPHVKSVVLPNGIRFNTIDVELFAPKFSYPSPGKLSGLIHDHFKEKYGEDSPILTLAYGPDFAVVRAADGMAAYGFDLNEIIPKLQEALPSAGIEGGGHSYAGSIKFFEGMRKEVLEEFAKQVVKLKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6W9</accession>
    <name>A0A218P6W9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphoesterase</fullName>
            <ecNumber evidence="4">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_03935</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="4"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853796.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6W9"/>
    <dbReference type="GeneID" id="33315392"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03935"/>
    <dbReference type="OrthoDB" id="9959at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00841">
        <property type="entry name" value="MPP_YfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053193">
        <property type="entry name" value="MetalloPDE_YfcE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041802">
        <property type="entry name" value="MPP_YfcE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020935">
        <property type="entry name" value="PdiEstase_YfcE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43165">
        <property type="entry name" value="METALLOPHOSPHOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43165:SF1">
        <property type="entry name" value="PHOSPHODIESTERASE MJ0936"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01269">
        <property type="entry name" value="UPF0025"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="5">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="170" mass="18769" checksum="F93076FC835EEB38" modified="2017-09-27" version="1">MLIGIMSDTHDNLPAIRKAVDLFNRENVELVIHAGDFVAPFVAWELKKLDAPLKGVFGNNDGERKGLYEALGIYDELIELEADGMKIAVTHGTNEVLVRALARSNLYDVVVVGHTHRYEIREEGRTILVNPGEVCGYVTGVKSVALLDTRKREVRIVNLDTGELLGAMSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Z4</accession>
    <name>A0A218P6Z4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_04005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853807.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Z4"/>
    <dbReference type="GeneID" id="33315405"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04005"/>
    <dbReference type="OrthoDB" id="9577at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586:SF15">
        <property type="entry name" value="BLR3095 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586">
        <property type="entry name" value="CYSTEINE DESULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="1">
        <location>
            <begin position="55"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Aminotransferase class V" evidence="1">
        <location>
            <begin position="275"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="392" mass="43381" checksum="77DED5F247752EE6" modified="2017-09-27" version="1">MRRSLFPALRKFRTYLNTAGLGLMPASALQRTNELLTDVIGFEGEVNSVDYMNPTFLEPTLREAAKLMRVSVENVSLSIQTTEGLRRLLFSMEPRKGQNVVSLDTEFPTTPALLKSYSERFGLELRVVKNENGLHPLETIERAIDDNTLALVLSSVNWVTGERINLRELSKVAHEHGAWLIIDAVQHLGSLRLYPEREGVDALAAGGEKWLLSPDTGAGLIYASDEFLEEAKPITGLLSMEPPTGDWGSWWGLPDKDPWGELKISRGTRKLDFGGGPPYIIAAAFRASLELINEIGIEEIERHNLKLAERVMEEALSAGLGVFGGNSAIVTVKTGMGYEAEEEVYRRLSERGIAVSHRGVLGHHGIRVSPHLYNTMEDVELFLEELFGTMGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P709</accession>
    <name>A0A218P709_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl29</name>
        <name evidence="6" type="ORF">A3L08_04080</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853821.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P709"/>
    <dbReference type="GeneID" id="33315420"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04080"/>
    <dbReference type="OrthoDB" id="11736at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00427">
        <property type="entry name" value="Ribosomal_L29_HIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
        <property type="entry name" value="Ribosomal_uL29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_uL29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018254">
        <property type="entry name" value="Ribosomal_uL29_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036049">
        <property type="entry name" value="Ribosomal_uL29_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00012">
        <property type="entry name" value="L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46561">
        <property type="entry name" value="Ribosomal protein L29 (L29p)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00579">
        <property type="entry name" value="RIBOSOMAL_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="11"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="66" mass="7861" checksum="D08647C29E979EEB" modified="2017-09-27" version="1">MKPSEIREMSLDEIEKKLRELRLELAKERGVLTMGASTENPMVIRNLRRDIARLLTIKKEKLREKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P712</accession>
    <name>A0A218P712_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rps3</name>
        <name evidence="10" type="ORF">A3L08_04075</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds the lower part of the 30S subunit head.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853820.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P712"/>
    <dbReference type="GeneID" id="33315419"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04075"/>
    <dbReference type="OrthoDB" id="9126at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02411">
        <property type="entry name" value="KH-II_30S_S3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1140.32:FF:000012">
        <property type="entry name" value="30S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.20:FF:000001">
        <property type="entry name" value="30S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="entry name" value="Ribosomal protein S3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01309_A">
        <property type="entry name" value="Ribosomal_uS3_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057258">
        <property type="entry name" value="Ribosomal_uS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027488">
        <property type="entry name" value="Ribosomal_uS3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_uS3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005703">
        <property type="entry name" value="Ribosomal_uS3_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003219">
        <property type="entry name" value="PRK04191.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01008">
        <property type="entry name" value="uS3_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760:SF32">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="Ribosomal protein S3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="KH type-2" evidence="9">
        <location>
            <begin position="17"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="209" mass="23274" checksum="0129B9EBD9585B9E" modified="2017-09-27" version="1">MAIERYFIKEGVKEMLIDEYLEKELRRAGYGGIDIKKTPLGTKVVIFAANPGYVIGRGGRKIRELTRILEREFGLENPQIEVEEIKNPYLNAKVQAVRLAQTLERGVHFRRAAYSAIRAIMGNGARGVEIRLSGKLTGERAKSVRFYQGYLAKVGNPAETLVSKGYAQAKLKLGVIGVKVSIMPPDAKLPDEIEVIEKVQEEVSTNEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P717</accession>
    <name>A0A218P717_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Small ribosomal subunit protein eS4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rps4e</name>
        <name evidence="10" type="ORF">A3L08_04110</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the eukaryotic ribosomal protein eS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853827.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P717"/>
    <dbReference type="GeneID" id="33315426"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04110"/>
    <dbReference type="OrthoDB" id="372073at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06087">
        <property type="entry name" value="KOW_RPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000090">
        <property type="entry name" value="30S ribosomal protein S4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00485">
        <property type="entry name" value="Ribosomal_eS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000876">
        <property type="entry name" value="Ribosomal_eS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013845">
        <property type="entry name" value="Ribosomal_eS4_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038237">
        <property type="entry name" value="Ribosomal_eS4_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041982">
        <property type="entry name" value="Ribosomal_eS4_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013843">
        <property type="entry name" value="Ribosomal_eS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018199">
        <property type="entry name" value="Ribosomal_eS4_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003312">
        <property type="entry name" value="PRK04313.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00900">
        <property type="entry name" value="Ribosomal_S4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08071">
        <property type="entry name" value="RS4NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002116">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00528">
        <property type="entry name" value="RIBOSOMAL_S4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 7 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 8" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="RNA-binding S4" evidence="9">
        <location>
            <begin position="44"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="243" mass="28017" checksum="FD75A2C38BA23242" modified="2017-09-27" version="1">MARKGAKRHLKRLAAPTQWYIHRKEYKWAVRPRPGPHSMKTSIPLLYIVRDYLGYARTAREARKILNEGKVLVDGRVRKDYKFPVGIMDVISIPETGEHYRVLPNRIGKLILHPISEKEANIKPLRISNKRMVKGAKVQLNLHDGSNHLVTMDEKDRYMTAYTVLMKVPEREVIEVLPFEVGSYVFVTQGKNVARKGKIVEVRQFPMGWPDVVTIEDENGELFDTLKEYAFVVGKDKPEISLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P728</accession>
    <name>A0A218P728_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Protein translocase subunit SecY</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Protein transport protein SEC61 subunit alpha homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">secY</name>
        <name evidence="15" type="ORF">A3L08_04165</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the Sec protein translocase complex. Heterotrimer consisting of alpha (SecY), beta (SecG) and gamma (SecE) subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. May interact with SecDF, and other proteins may be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10 13">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853837.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P728"/>
    <dbReference type="GeneID" id="33315437"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04165"/>
    <dbReference type="OrthoDB" id="371914at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3370.10:FF:000013">
        <property type="entry name" value="Protein translocase subunit SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="entry name" value="SecY subunit domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01465">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026593">
        <property type="entry name" value="SecY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030659">
        <property type="entry name" value="SecY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019561">
        <property type="entry name" value="Translocon_Sec61/SecY_plug_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00967">
        <property type="entry name" value="3a0501s007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006341">
        <property type="entry name" value="PRK08568.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="SECY/SEC61-ALPHA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10559">
        <property type="entry name" value="Plug_translocon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00303">
        <property type="entry name" value="SECYTRNLCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="Preprotein translocase SecY subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00755">
        <property type="entry name" value="SECY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00756">
        <property type="entry name" value="SECY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="156"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="296"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="419"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="443"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Translocon Sec61/SecY plug" evidence="14">
        <location>
            <begin position="39"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF10559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ASJ06579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="482" mass="53506" checksum="741D0D35C5ADF86D" modified="2017-09-27" version="1">MGKARDIIYALERYFPEVERPKRHVPLKEKFMWTGLALLLYFVLAEIPLYGIPENIQDYFATLRFVLAGRSGSLLTLGIGPIVTASIIMQLLVGSEIVHLDLSNHEDRRFYQAAQKLFAVFMSFFEAAIYVFAGAFGKVDTGIGAFQTVSTPGGAVYIGLGLGILIILQLGFASTLLILLDELVSKWGIGSGISLFIAAGVSQTVIVKSLNPFTTSQYIDPVTGGPAIIGAIPAFIQHLIHGDLTGALYRGTLPDIMDVLATIVVFLVVVYLESMRVEIPLSYGRVTVRGRYPIRFMYVSNIPIILTFALYANVQLWARLLNNYGYTFLGTFDQNGNPLSGLVTYLYPPRDIYHVIADPGRALVYALMTIFWAIIFGFLWVELTGLDAKSIARQLQNAGLQIPGFRRDPRILERVLNRYIPYVTFWGSFTLAIVAVLADFLGALGTGTGILLTVGILYRFYEEIAREQATEMFPALRKFFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P730</accession>
    <name>A0A218P730_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF106 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_04175</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853839.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P730"/>
    <dbReference type="GeneID" id="33315439"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04175"/>
    <dbReference type="OrthoDB" id="84619at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002809">
        <property type="entry name" value="EMC3/TMCO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038978">
        <property type="entry name" value="MJ0935"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42198">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42198:SF1">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01956">
        <property type="entry name" value="EMC3_TMCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01415">
        <property type="entry name" value="DUF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="175" mass="20742" checksum="9FA21AF7DD252E7E" modified="2017-09-27" version="1">MIEGIYSFLDSVFGPLIQAYHPIVVVTIAGAILGVFFTLMNYFLVDQEKTKLLQKKSKEFQKKYREAQASKDEKKLRKLQQEQMELMRLQSEVMKDTMFKVTLLTLPIFWIFFGWLRRWYVEVGIAKAPFNFFVFDWFHRMYHSGLPASELGYIGWYFLTSYAVGMILRKLLDMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P737</accession>
    <name>A0A218P737_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">rpl2p</name>
        <name evidence="7" type="synonym">rpl2</name>
        <name evidence="11" type="ORF">A3L08_04060</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853818.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P737"/>
    <dbReference type="GeneID" id="33315416"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04060"/>
    <dbReference type="OrthoDB" id="5987at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000001">
        <property type="entry name" value="50S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000020">
        <property type="entry name" value="60S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.950.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="entry name" value="Ribosomal protein L2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01320_A">
        <property type="entry name" value="Ribosomal_uL2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_uL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023672">
        <property type="entry name" value="Ribosomal_uL2_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_uL2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_uL2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Ribosomal_uL2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007180">
        <property type="entry name" value="PRK09612.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="RIBOSOMAL PROTEIN L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002158">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7 11" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL2 RNA-binding" evidence="10">
        <location>
            <begin position="11"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL2 C-terminal" evidence="9">
        <location>
            <begin position="91"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="200"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="222"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00065832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="239" mass="25966" checksum="66D4F444F9C48012" modified="2017-09-27" version="1">MGKSLIQQRRGKGTTTFRAPSHRYRGAVRYVPLNITKEKTLVGKVVEILHDPGRTAPVARVKFENGMEKLIIAPEGILVGEEIAIGPNAPVKIGNTLPLAMIPEGSYVYDIEGVPGDGGKYVRAGGAYALVVSREKDKVIVQLPSGELKQFKPECRATIGVVAGGGRLEKPIVKAGKAYYIAKARNRFWPKPRGVKMNAVNHPHGGKEHHIGRPSTVSRRAPPGRKVGHIAARRTGRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7J2</accession>
    <name>A0A218P7J2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Ribosome biogenesis protein Nop10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nop10</name>
        <name evidence="8" type="ORF">A3L08_05145</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Involved in ribosome biogenesis; more specifically in 18S rRNA pseudouridylation and in cleavage of pre-rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NOP10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853994.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7J2"/>
    <dbReference type="GeneID" id="33315633"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05145"/>
    <dbReference type="OrthoDB" id="7259at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.40">
        <property type="entry name" value="H/ACA ribonucleoprotein complex, subunit Nop10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00803">
        <property type="entry name" value="Nop10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007264">
        <property type="entry name" value="H/ACA_rnp_Nop10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036756">
        <property type="entry name" value="H/ACA_rnp_Nop10_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023532">
        <property type="entry name" value="Nop10_arc-typ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009623">
        <property type="entry name" value="PRK13130.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13305:SF0">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13305">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN NOP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04135">
        <property type="entry name" value="Nop10p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144210">
        <property type="entry name" value="Nop10-like SnoRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 7" id="KW-0698">rRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="59" mass="6954" checksum="C1DA00950EBB4A2C" modified="2017-09-27" version="1">MHFRIRKCPSCGRYTLKETCPVCGEKTKVAHPPRFSPEDPYGEYRRRLKREQMGIARGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7V0</accession>
    <name>A0A218P7V0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05790</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854117.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7V0"/>
    <dbReference type="GeneID" id="33315762"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05790"/>
    <dbReference type="OrthoDB" id="114814at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19067">
        <property type="entry name" value="PfuEndoQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005287">
        <property type="entry name" value="CHP00375"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00375">
        <property type="entry name" value="TIGR00375 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40084">
        <property type="entry name" value="PHOSPHOHYDROLASE, PHP FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40084:SF1">
        <property type="entry name" value="PHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="420" mass="47699" checksum="B9B7DE6192DB1464" modified="2017-09-27" version="1">MQVDADLHIHSRYSKAVSKAMTIHNLAENARFKGLGLVGTGDILNPKWEEELLKYTEKVDEGTYERNGIRFLLTTEVEDNRRVHHVLIFPAIEAVREMRERLKPYSSDIETEGRPHLSLSAGEIADLANELDVLIGPAHAFTPWTSLYKEYDSLKEAYGGAKIHFLELGLSADSEMADMIKAHHKLTYLSNSDAHSPMPHRLGREFNRFEVKEVTFEEIRKVILKRGGRRIVLNAGLDPRLGKYHLTACSRCYTKYSLEEAKAFHWKCPRCGGRIKKGVHDRILELADTNERPKDRPPYIHLAPLAEIIAMVIGKGVETKAVRIIWERFLQEFGSEIRVLVDVPVETLAQVHEDVAKAVWAYRNDKLIIIPGGGGKYGEIRLPEEIRNASIDELESVEVELPAEEKPKQRSITEFLGVKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7V3</accession>
    <name>A0A218P7V3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutamyl-tRNA(Gln) amidotransferase subunit E</fullName>
            <shortName evidence="6">Glu-ADT subunit E</shortName>
            <ecNumber evidence="6">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">gatE</name>
        <name evidence="9" type="ORF">A3L08_05700</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 11">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of GatD and GatE.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GatB/GatE family. GatE subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854099.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7V3"/>
    <dbReference type="GeneID" id="33315744"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05700"/>
    <dbReference type="OrthoDB" id="7316at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.410:FF:000003">
        <property type="entry name" value="Glutamyl-tRNA(Gln) amidotransferase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.380:FF:000002">
        <property type="entry name" value="Glutamyl-tRNA(Gln) amidotransferase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.30:FF:000003">
        <property type="entry name" value="Glutamyl-tRNA(Gln) amidotransferase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="entry name" value="GAD-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.380">
        <property type="entry name" value="GatB domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00588">
        <property type="entry name" value="GatE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029351">
        <property type="entry name" value="GAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042114">
        <property type="entry name" value="GatB_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004414">
        <property type="entry name" value="GatE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00134">
        <property type="entry name" value="gatE_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003107">
        <property type="entry name" value="PRK04028.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="GLUTAMYL-TRNA GLN AMIDOTRANSFERASE SUBUNIT B MITOCHONDRIAL AND PROKARYOTIC PET112-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659:SF2">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02938">
        <property type="entry name" value="GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55261">
        <property type="entry name" value="GAD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="GatB/YqeY motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Asn/Gln amidotransferase" evidence="8">
        <location>
            <begin position="484"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="411"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="629" mass="71161" checksum="BEF3673C70257B0F" modified="2017-09-27" version="1">MAEKFNYEELGLKVGLEIHRQLDTKKLFSPVPSEFSEDVDFTFERRLRPTMSELGEIDPAALEEFKKGKVYVYEGNYELTDLVYMDEEPPRGPDEEALKTALQIVYLLNAVPVDEVHFMRKIVIDGSNVSGFQRTAIVGMEGKVDTPWGSVGIPTVCLEEDACRIVERGEKKVIYRLDRLGVPLVEIATTPDIHHPEQAKVVAKFIGDALRATRKVKRGLGTIRQDLNVSIRDGARVEIKGVQELDMIPLIIEREVERQVNLLKIRDELRERGVKPEDLNEEFHDVTDVFQNTGSKIIARAIKNGGKVLAVKLPGFRGLIGREIQPGRRLGTEMANRARKYVRGIFHIDELPNYGITELEVNAVIEKLNLGENDAFVLVAAEEETARKALREVVKRAKEAIEGVPEETRRALPDGNTEYMRPLPGKARMYPETDVPPIEITEELKRSIIENLPELPQERVERFVREYRIDKSLAETLVNDERDELFEELVKKGLKPSLVASILVVVLKGLKKEAPIENLTEEHIREAFELYIEGKIAKEAFEEIFKELAKNPTKTARQVAEEKGLTLLSEEEVERIIDEVVQQNIDVIKAKGMGAMGMIMGRAMAKLRGRADGKLVNQLVRKKIQEIAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7V4</accession>
    <name>A0A218P7V4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Voltage-gated chloride channel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">A3L08_05795</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854118.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7V4"/>
    <dbReference type="GeneID" id="33315763"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05795"/>
    <dbReference type="OrthoDB" id="89900at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00400">
        <property type="entry name" value="Voltage_gated_ClC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050368">
        <property type="entry name" value="ClC-type_chloride_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43427">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN CLC-E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43427:SF6">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN CLC-E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0129">CBS domain</keyword>
    <keyword evidence="8" id="KW-0868">Chloride</keyword>
    <keyword evidence="7" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="154"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="185"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="362"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="392"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="12">
        <location>
            <begin position="448"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="12">
        <location>
            <begin position="510"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="572" mass="61708" checksum="1CB4DB5E2D69BF4B" modified="2017-09-27" version="1">MARRKHYLRKWAEVLVFSFMAGLIGGLGAVAFRFLISLTHEFFFGKVLSLVTLQAGSFNLGYILIPATGALIVGFIVLRYPEIKGNGIPEVIEAVVFRNGNIPGSFAVAKIVATSITIGSGGSVGREGPIGFIGASLTSIFARWFGLSREMKKLLVTCGLAAGIAGTFNTPLAGAMFALEVVYMGAFSINLVPIFLSAITGNAVTLALLNKAFEVEIPANLGHTLVELPFFFIFGIILGLLAAFYARFLYGVSDRFSASKIPEWAKPFLGGLGVGLISFLFPGYGVFGIGYDGMKLAFYGKLGIGLLLTLGLAKMLATALTIGSGQSGGVFAPSLYIGTMFGAAFGEAINLLLPSLQADPSVYALAGMAAFFSGMTQAPLTQILMVTELTRSYAVLPAVMTSATMGFLTARFFLGGESIYTLKLLRKGYHIKTGKPVILETISVGEIMTENPVYVTEDMTLFDVEHLIGETGHDCFPVVNERMEVIGVIGVKDILKKSSSMKRMRVKRFLKRAYGITYPTETAQDALEKLMAYDQNLLPVVESPQSRRLVGVVTKRDIYRAYYRGLEGMYID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Y7</accession>
    <name>A0A218P7Y7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 8">Glutamyl-tRNA(Gln) amidotransferase subunit D</fullName>
            <shortName evidence="5">Glu-ADT subunit D</shortName>
            <ecNumber evidence="5 8">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">gatD</name>
        <name evidence="12" type="ORF">A3L08_05705</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 8">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5 8">Heterodimer of GatD and GatE.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 8">Belongs to the asparaginase 1 family. GatD subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="5 8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Y7"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05705"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08962">
        <property type="entry name" value="GatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="entry name" value="L-asparaginase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00586">
        <property type="entry name" value="GatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006033">
        <property type="entry name" value="AsnA_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020827">
        <property type="entry name" value="Asparaginase/glutaminase_AS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027475">
        <property type="entry name" value="Asparaginase/glutaminase_AS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011878">
        <property type="entry name" value="GatD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040918">
        <property type="entry name" value="GatD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037222">
        <property type="entry name" value="GatD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00519">
        <property type="entry name" value="asnASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02153">
        <property type="entry name" value="gatD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003217">
        <property type="entry name" value="PRK04183.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707:SF28">
        <property type="entry name" value="60 KDA LYSOPHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18195">
        <property type="entry name" value="GatD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500175">
        <property type="entry name" value="Glu_ADT_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00139">
        <property type="entry name" value="ASNGLNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141300">
        <property type="entry name" value="GatD N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00144">
        <property type="entry name" value="ASN_GLN_ASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00917">
        <property type="entry name" value="ASN_GLN_ASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 5 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 5 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GatD N-terminal" evidence="11">
        <location>
            <begin position="15"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="L-asparaginase N-terminal" evidence="9">
        <location>
            <begin position="95"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase/glutaminase C-terminal" evidence="10">
        <location>
            <begin position="307"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 7">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="439" mass="49014" checksum="A0F233D482D36261" modified="2017-09-27" version="1">MRKVEAFMKEKGLSLGDRVSVVKREGDRRIRYEGIVMPPYELSPGETLTIKLDNGYNVGVRIELIESVEVIEKARPGEEVAFKEVLPRKEGLPGVTILGTGGTIASKIDYKTGAVHAAFTAEELARAVPEIFEIANITPKLLMNIMSEDMKPDYWKRMAHEAAKALNAGEDGVVIAHGTDTLAYSAAALSFMLRDLEKPVIFVGSQRSSDRPSSDSAMNLICSVRMATADFGEVAIVMHGETSDTYCLAHRGTKARKMHTSRRDAFRSINDVPIARVWPKGEIEFLRKDYRKRGEREVWVDDRMEPRVGLLKVVPGISSELIDFFVDRGYRGLVIEGTGLGHTPNDIIPAIERATEEGMAVCMTSQCLYGRVNLNVYSTGRRLLKAGVIPCEDMLPETAYVKLMWVLGHTDDPKEVREMMLTNYAGEITPYTRFDTYLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Z6</accession>
    <name>A0A218P7Z6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06055</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854165.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Z6"/>
    <dbReference type="GeneID" id="33315814"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06055"/>
    <dbReference type="OrthoDB" id="84888at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="166" mass="19683" checksum="F52DC7A944E2CE75" modified="2017-09-27" version="1">MTGTVSKIIHFHDEEEFLDDMSAAMERFSYLASKYGHNPIEGVLLWDYVGVRDEEGIKIFRVGEFPYFEGTLKVDLETLRVMERYFDEMESKWDELRVEDIAYFVEMLNEALGEERVYYEAYDLGLDRNTAYIILNIANLHYLESVLDGRDREIFEEAVELLMRYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P839</accession>
    <name>A0A218P839_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF996 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P839"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06160"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010397">
        <property type="entry name" value="DUF996"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06195">
        <property type="entry name" value="DUF996"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="210" mass="22916" checksum="CA475ECFAF06079A" modified="2017-09-27" version="1">MTVNVRSEKNLGMWGSILALLGGFVPYIGSVLALVGSILVLVALHGIGNAVGDDRPFKNYLFAFIVGIAVLVLFIFVIFATFGLTMSGLSVHEEFSGPITAAPSESMSITNEYTEALPLMIISGVFLLVVLITVVNAYFEMKAWSAMYEITRTKSFEDAANWFKWGAITAIVLVGFLLIFIARIFVILGFSNMPDELEEKPQLPLDSHIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P840</accession>
    <name>A0A218P840_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">lipoate--protein ligase</fullName>
            <ecNumber evidence="3">6.3.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_06280</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-lysyl-[lipoyl-carrier protein] + (R)-lipoate + ATP = N(6)-[(R)-lipoyl]-L-lysyl-[lipoyl-carrier protein] + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49288"/>
            <dbReference type="Rhea" id="RHEA-COMP:10500"/>
            <dbReference type="Rhea" id="RHEA-COMP:10502"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoate: step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoate: step 2/2.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.20" evidence="3"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854206.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P840"/>
    <dbReference type="GeneID" id="33315859"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06280"/>
    <dbReference type="OrthoDB" id="146287at2157"/>
    <dbReference type="UniPathway" id="UPA00537">
        <property type="reaction ID" value="UER00594"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016979">
        <property type="term" value="F:lipoate-protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.50">
        <property type="entry name" value="CO dehydrogenase flavoprotein, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019491">
        <property type="entry name" value="Lipoate_protein_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050664">
        <property type="entry name" value="Octanoyltrans_LipM/LipL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43679:SF2">
        <property type="entry name" value="OCTANOYL-[GCVH]:PROTEIN N-OCTANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43679">
        <property type="entry name" value="OCTANOYLTRANSFERASE LIPM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10437">
        <property type="entry name" value="Lip_prot_lig_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82649">
        <property type="entry name" value="SufE/NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoate protein ligase C-terminal" evidence="8">
        <location>
            <begin position="7"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="92" mass="10835" checksum="3EF0F2B4CA98D93E" modified="2017-09-27" version="1">MKHRVGEHKAKKGLIRIEFDEENGIAEHVRITGDFFMHPEEAVHELEQKLEGHRLEELEGLMDEFFAMRMDIETPYVNVEDFKIALKNALKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P842</accession>
    <name>A0A218P842_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06235</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854197.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P842"/>
    <dbReference type="GeneID" id="33315850"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06235"/>
    <dbReference type="OrthoDB" id="30884at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014424">
        <property type="entry name" value="UCP004897_ACT"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004897">
        <property type="entry name" value="UCP004897_ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="166" mass="18794" checksum="9FFC9E3C8CF73631" modified="2017-09-27" version="1">MWGKIEHYFDEYPVRKQIAKTLLKYGLKVSDDLRIKAGDIEVPYTKIAKALDVDRRVVKETVGMILKIPELKEIYTNLEPTVHMKYVGRHVGYGVIEIEPEPRAVGILAKIAQKIADRGVNIVQVVAEDPELYPEATLTIITEKPIPGDLINELSKLEGVKRISIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P869</accession>
    <name>A0A218P869_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF2666 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06435</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854234.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P869"/>
    <dbReference type="GeneID" id="33315890"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06435"/>
    <dbReference type="OrthoDB" id="86044at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022620">
        <property type="entry name" value="DUF2666"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10869">
        <property type="entry name" value="DUF2666"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2666" evidence="1">
        <location>
            <begin position="4"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2666" evidence="1">
        <location>
            <begin position="138"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="267" mass="29929" checksum="AF4FB9725F42D335" modified="2017-09-27" version="1">MKVEDQIVFTAHHGSWKVADRLLDMEDEKVAHFIASVANTVNAKIPEYLTEVMNVAGIMSLAEELAKKNLADAVVALKSPGTARKLGGLVFEEDKKLKKLLTDVAKAVLAREVLSRKVPVDYPEGPLTEVRIVFPYSEDHVNFTAFHLSAEHGPWRAVRRLIIDDKTPMADVARLLASINESITLKLPVYAGIDVDGIDTWFGEFKKVTKADIPTVVEKYRHFPAENYAPDHFIEHARVYALRKALEKVGIPFDVPAKSLEKYLEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8B1</accession>
    <name>A0A218P8B1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Haloacid dehalogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_06660</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854275.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8B1"/>
    <dbReference type="GeneID" id="33315935"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06660"/>
    <dbReference type="OrthoDB" id="26985at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14820">
        <property type="entry name" value="TRAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002848">
        <property type="entry name" value="Translin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036081">
        <property type="entry name" value="Translin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011161">
        <property type="entry name" value="PRK14562.1-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10741">
        <property type="entry name" value="TRANSLIN AND TRANSLIN ASSOCIATED PROTEIN X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01997">
        <property type="entry name" value="Translin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74784">
        <property type="entry name" value="Translin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="3"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR602848-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="212" mass="24572" checksum="C2A17D35E27B0A74" modified="2017-09-27" version="1">MELKEIIAEIRKVLDEKDSLREEALRLTREIVRLSGDTIKALHRGETEKARERLKVAREKVEKLKEKLREHPDLYYTGYVQSAHQEFVEASLFFAYMTGSEFPSPAELGVPHADYALGVGDLIGELRRHFLLLLLDGKLDEAEKTYRFMESVYEELMTLEYPKGLVNVRGKQDQARGILERTLEDLTRAKLNARLESRLRNAVEALENRENQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8B5</accession>
    <name>A0A218P8B5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Proline--tRNA ligase</fullName>
            <ecNumber evidence="10">6.1.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Prolyl-tRNA synthetase</fullName>
            <shortName evidence="10">ProRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">proS</name>
        <name evidence="12" type="ORF">A3L08_06695</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>tRNA(Pro) + L-proline + ATP = L-prolyl-tRNA(Pro) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.15" evidence="10"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854281.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8B5"/>
    <dbReference type="GeneID" id="33315942"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06695"/>
    <dbReference type="OrthoDB" id="7375at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00862">
        <property type="entry name" value="ProRS_anticodon_zinc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00778">
        <property type="entry name" value="ProRS_core_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000005">
        <property type="entry name" value="bifunctional glutamate/proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000037">
        <property type="entry name" value="Proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.30">
        <property type="entry name" value="C-terminal domain of ProRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01571">
        <property type="entry name" value="Pro_tRNA_synth_type3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002316">
        <property type="entry name" value="Pro-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004499">
        <property type="entry name" value="Pro-tRNA-ligase_IIa_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016061">
        <property type="entry name" value="Pro-tRNA_ligase_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017449">
        <property type="entry name" value="Pro-tRNA_synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033721">
        <property type="entry name" value="ProRS_core_arch_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00408">
        <property type="entry name" value="proS_fam_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382:SF2">
        <property type="entry name" value="BIFUNCTIONAL GLUTAMATE_PROLINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382">
        <property type="entry name" value="PROLYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09180">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01046">
        <property type="entry name" value="TRNASYNTHPRO"/>
    </dbReference>
    <dbReference type="SMART" id="SM00946">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64586">
        <property type="entry name" value="C-terminal domain of ProRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 10 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="11">
        <location>
            <begin position="42"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ07031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="483" mass="56215" checksum="BA0AA64152D27577" modified="2017-09-27" version="1">MGGKVKREKWSENFSEWYNELIETAGIQDKRYPVKGMNIWLPYGLKIMRNIERFIHSEMERTGHDEVLFPALIPETEFQKEAEHIKGFEGEVYWVTHAGLDPLDVRLILRPTSETAMYSMFSLWIRSHADLPFKVYQIVNTYRYETKHTRPLIRVREISRFFEAHTAHDSYEDAERQIREDLEIFDRLAKFLAIPYIVSKRPDWDKFPGAYYSLGAEVMMPDGRTLQIGTMHNYRQNFAKAYNIQYETEEGEHEYVHQTTFGMSERLLAAVMAIHGDDNGLVLPPTIAPIQVVIVPIPKKDAEVDVFAYAREIAEELRDAGIRVHVDERDIRPGRKYYDWELKGVPVRIEVGPKDVEGGKAVIARRDTLTKEVVERAEIVEAVRRTFDEIMENLYNRAREFLESHIKRVDTIEEAKGVFENRRGIVEIPWCGEEECGLKMEEELDAKMLGIPYPEETAKAPEGKKCPVCGREAKFIARFARTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8E1</accession>
    <name>A0A218P8E1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">AbrB family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06795</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854298.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8E1"/>
    <dbReference type="GeneID" id="33315962"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06795"/>
    <dbReference type="OrthoDB" id="28233at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16321">
        <property type="entry name" value="MraZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035644">
        <property type="entry name" value="MraZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020603">
        <property type="entry name" value="MraZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02381">
        <property type="entry name" value="MraZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51740">
        <property type="entry name" value="SPOVT_ABRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="3"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="72" mass="8815" checksum="7A5AA2BA34418E17" modified="2017-09-27" version="1">MEVVVKRIDPQGRILLPKEIREKLGEEVILVDLGDRVEILPRRKWDLTRFFDTVEVEELKEWEELKKELWER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8H0</accession>
    <name>A0A218P8H0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thiamine thiazole synthase</fullName>
            <ecNumber evidence="6">2.4.2.59</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">thi4</name>
        <name evidence="7" type="ORF">A3L08_06895</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>hydrogen sulfide + glycine + NAD(+) = ADP-5-ethyl-4-methylthiazole-2-carboxylate + nicotinamide + 3 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:139151"/>
            <dbReference type="EC" id="2.4.2.59"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homooctamer; tetramer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the THI4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.59" evidence="6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854318.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8H0"/>
    <dbReference type="GeneID" id="33315982"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06895"/>
    <dbReference type="OrthoDB" id="4240at2157"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00304">
        <property type="entry name" value="Thi4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002922">
        <property type="entry name" value="Thi4_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022828">
        <property type="entry name" value="Thi4_prok"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00292">
        <property type="entry name" value="sulfide-dependent adenosine diphosphate thiazole synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43422">
        <property type="entry name" value="THIAMINE THIAZOLE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43422:SF3">
        <property type="entry name" value="THIAMINE THIAZOLE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01946">
        <property type="entry name" value="Thi4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <begin position="54"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="152"/>
            <end position="154"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>glycine</name>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="251" mass="26899" checksum="6027CCDE35DB9347" modified="2017-09-27" version="1">MLKDVEISRAIVEAYFNDLLENLSLDVAIVGAGPSGMVAGYYLAKNGAKVAIFEKKLSIGGGIWGGAMGFNRIVVQEEAKEILDEFGIRYRPFKNGLYVADAIETATTLASRAVKAGVKVFNMIEVEDLVVKENRVAGIVINWTPVKMTGLHVDPLTVEAKFVVDSTGHGAQISQHLLKRGLIEKIPGEGPMWAEKGEELTVKHTGEVFPGLYVTGMAANALAGAPRMGPIFGGMFLSGRKAALEILQKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8H1</accession>
    <name>A0A218P8H1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Bifunctional thiamine biosynthesis protein ThiDN</fullName>
            <ecNumber evidence="6">2.5.1.3</ecNumber>
            <ecNumber evidence="5">2.7.1.49</ecNumber>
            <ecNumber evidence="7">2.7.4.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">A3L08_06890</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P.</text>
    </comment>
    <comment type="function">
        <text evidence="17">Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = thiamine phosphate + CO2 + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:47848"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:62890"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = thiamine phosphate + CO2 + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:47844"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + ATP = 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + ADP</text>
            <dbReference type="Rhea" id="RHEA:19893"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:58354"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.7"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-amino-5-hydroxymethyl-2-methylpyrimidine + ATP = 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23096"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16892"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58354"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.49"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>4-methyl-5-(2-phosphooxyethyl)-thiazole + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + H(+) = thiamine phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22328"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:58296"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="18">Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">In the C-terminal section; belongs to the ThiN family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="20">In the N-terminal section; belongs to the ThiD family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.3" evidence="6"/>
    <dbReference type="EC" id="2.7.1.49" evidence="5"/>
    <dbReference type="EC" id="2.7.4.7" evidence="7"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854317.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8H1"/>
    <dbReference type="GeneID" id="33315981"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06890"/>
    <dbReference type="OrthoDB" id="43786at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008902">
        <property type="term" value="F:hydroxymethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008972">
        <property type="term" value="F:phosphomethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004789">
        <property type="term" value="F:thiamine-phosphate diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01169">
        <property type="entry name" value="HMPP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000015">
        <property type="entry name" value="Phosphomethylpyrimidine kinase (Hmp-phosphate kinase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004399">
        <property type="entry name" value="HMP/HMP-P_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019293">
        <property type="entry name" value="ThiN"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00097">
        <property type="entry name" value="HMP-P_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858:SF17">
        <property type="entry name" value="HYDROXYMETHYLPYRIMIDINE_PHOSPHOMETHYLPYRIMIDINE KINASE THI20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858">
        <property type="entry name" value="PHOSPHOMETHYLPYRIMIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10120">
        <property type="entry name" value="ThiN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 24" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyridoxamine kinase/Phosphomethylpyrimidine kinase" evidence="22">
        <location>
            <begin position="10"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine-phosphate synthase ThiN" evidence="23">
        <location>
            <begin position="258"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00059241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00060674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00060834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00060992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00067160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF10120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="ASJ07067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="428" mass="47189" checksum="D08B1DD16E0D8ACF" modified="2017-09-27" version="1">MAVLIVAGLDTGGGAGLKADIETVSALGEHPLPVLTSVTYQNPSEVRGYHTLPPEVVREQIRAVRGSFKIGAVKIGMLGSREIAEAVKQETEGLTRVLDPVMESSTGAKLVDSVESVKVLILGSIVTPNVPEAEALSGLKIRSIEEMKEAAKVIAEELGAEAVVIKGGHLSYTDVLYWKGKLYEFPGERVEGFTHGTGCVFSSALAVFLAKGFELPETVERAKRFVEGSIRFSKAEARAVNPLWELERDAHRWRAKGELEEAVKELMELGEKLNPHVPEVGTNFALATPFSEVFAVKGRIVRYGGTIKPVGPVEFNASDHLRRALLKMREFYPEVRAVLNLRYSEELVEKAKKLGLVVSFYDRREEPEDVKRAEKGTMEWGIESAVKRVGKRPDLIYHLGDWGKEPMVLIFGRSAMEVVERVRALSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8I5</accession>
    <name>A0A218P8I5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854355.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8I5"/>
    <dbReference type="GeneID" id="33316024"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07105"/>
    <dbReference type="OrthoDB" id="85889at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="443" mass="49174" checksum="CFBE69FF0CE20D2E" modified="2017-09-27" version="1">MSSWWIYGYPEEGLRFRVADFLKKVTAVLILVWIFREPLRLEAYLEYIVWAIIGLIFAYELLGVGKWLGVTVSGVIFALAKAFFWVSVLLFVGSRWIGLPENLSGDDALLTAGTAFAYFVVLSIAGLLVGRTPFRRLSSRRWFTVERKAYSFSGADFGDVRLSGSGKAYPVKVGRKTVGWVIDGEVEVEAETPLGRVKKLLSPPVVVWTSRKLGGAKEKADPTFVERVQAMLNPDRLYRNKERAKVVDLGVVKVYEGDGFQYVKLPFVEVVETPHGEKVKVGPIHVHDGKPVSPPEDMVTIRELGNGFQLTKAGDRLAIETEEYSIEVVGDSVTYRSGEEALRLSRDYVSLKSGELSITVGKRRAKLRIDDTVITASDGKVRIRVGGKSYTIVSQEACQLVIGKAKEIVEEQSAELIEGFGVNRAALNSRVKELIDELMAYLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8L0</accession>
    <name>A0A218P8L0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06965</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8L0"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06965"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.180">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013319">
        <property type="entry name" value="GH11/12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="1077" mass="121015" checksum="F81BBF21882356E2" modified="2017-09-27" version="1">MGHFRKILGIWMALIILGTYVSGLTLPEARQIADNAESGDVALQWVPGAWPGELVTRKDGTTLWMETNFWNIQSGSGENYMRFIKNTEEVAFYVNMSGVVTNWGGNAFATPNVIFAGRAPAQWWGNKPTVGGYSWFSLPLGGDQINTLGSVWAKVDFELAKSPETLSRTGIIMWFEQPNGAPLGEVYIAFSDDYGGIVNTQWPPTDIGTITQTVYINGQARKVVFKIQRTWNWDHFVLEFIPDQEIPEGTIFIDVKQFAEAAVQGIVDDPYSSWTADQITWTSIGFGTYTGSNTDKFELGWILHDARLVESPLTLKVAKEIASTAESGDIVLQWSPDNPWPGELVTGEDGTTLWMETNFWNIVSGDGESYMRFISAGDSVAFYVNLSNVQTRWGGIAWATPAVVFAGRVPPSVTDGNELPSAGGYDYFSLPMPAVNVSNYNKILTKIDFELVKGEGTLVRPGIIMWFERSSDNYQLAEVYVPFFDDYNGIVGAQYGPWEVGTIEATVTINGVEKNVTFTVQKAWNWNNFAFEFIPSEPIAKGSGDVTIDLRPIVDKVIEEIIADQYSDYSENDIVWSSLAFGTYSGSDGTGFNYGWILHEAKVLPPKSEGTVESRESEIFYKIYVMWNYYNYYRYNKIREAYNKLLTNLTYEIPENILNTTEELRNRAEEDYQRAFELSSQPFYGGVANPRSFAYLRRAYLSIKNAEEILDKVIKYSQLAEEYKALLDEAKARNLSDEVLAEAENAGEKAADEYQKALSLPPGDPEIIDHVNEAYRFLEYATGILRDYITSLPVLEIKDLASEQDVGDVQFTWWDQWLEKPIKRSDGTTVWMQTNFWNIVDGQGEVYMRFIDASDSFAFYVDLENVKTQWGGIAWATPEVIIAGRAPAVWWGDKPPVGGYGFFDLPMPSSEISNYDHIWVKADYKVVKKEGLVRFGLLLWFENAEGWPVAEVYVPFFDDFGGLVGVSDKTTLGGINTTVNLNGETLEVTLKGEKGWNPGEWWSFEFIPQEQLPASGEIIIDIKPVVDEVIRQLSNQEITWTSISISSYSGSEGTAFNYGWILEDARVLPPEEAPTLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8M3</accession>
    <name>A0A218P8M3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL33</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl35ae</name>
        <name evidence="5" type="ORF">A3L08_07205</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL33 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854375.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8M3"/>
    <dbReference type="GeneID" id="33316044"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07205"/>
    <dbReference type="OrthoDB" id="14403at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.190">
        <property type="entry name" value="translation elongation factor selb, chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00573">
        <property type="entry name" value="Ribosomal_eL33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001780">
        <property type="entry name" value="Ribosomal_eL33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018266">
        <property type="entry name" value="Ribosomal_eL33_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038661">
        <property type="entry name" value="Ribosomal_eL33_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003326">
        <property type="entry name" value="PRK04337.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01247">
        <property type="entry name" value="Ribosomal_L35Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01105">
        <property type="entry name" value="RIBOSOMAL_L35AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="88" mass="9874" checksum="A760F17FE9FB33AF" modified="2017-09-27" version="1">MARVKALVLAYAGTKEHQDNHHMILKPLGIDDRNEASRLIGRKVVWRTPAGRKMFGKILRPHGNRGEVKAYFKPGLPGQALGDYVEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8N0</accession>
    <name>A0A218P8N0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF4870 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_07235</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854380.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8N0"/>
    <dbReference type="GeneID" id="33316050"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07235"/>
    <dbReference type="OrthoDB" id="329551at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019109">
        <property type="entry name" value="MamF_MmsF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36460">
        <property type="entry name" value="UPF0132 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_3G10255)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36460:SF1">
        <property type="entry name" value="UPF0132 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_3G10255)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09685">
        <property type="entry name" value="MamF_MmsF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="120" mass="13742" checksum="38CA1CC49C308FDF" modified="2017-09-27" version="1">MVEIPSEEPRKTSLGMDENVEGLLAYLLWWVTGIIFLLLEKDSDFVRFHAMQSTITFLGITVLMWLLAIIPFIGWVLANLLGLLGIILWIIGMVKAYQGERYKFPIVGDLAEQWMDKVNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Q1</accession>
    <name>A0A218P8Q1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1931 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_07425</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854413.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Q1"/>
    <dbReference type="GeneID" id="33316088"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07425"/>
    <dbReference type="OrthoDB" id="99090at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="59" mass="6646" checksum="BBEC32AD1DB933AC" modified="2017-09-27" version="1">MAEMIVKSKVKEAVKAIDPEMRVSPEFYDALEAELKALVEKAVKRAQAEGKKTLYARHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8R9</accession>
    <name>A0A218P8R9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_07485</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854424.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8R9"/>
    <dbReference type="GeneID" id="33316100"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07485"/>
    <dbReference type="OrthoDB" id="36796at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17923">
        <property type="entry name" value="DEXHc_Hrq1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18797">
        <property type="entry name" value="SF2_C_Hrq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018973">
        <property type="entry name" value="MZB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47957">
        <property type="entry name" value="ATP-DEPENDENT HELICASE HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47957:SF3">
        <property type="entry name" value="ATP-DEPENDENT HELICASE HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09369">
        <property type="entry name" value="MZB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="3">
        <location>
            <begin position="56"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="4">
        <location>
            <begin position="262"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="894" mass="103403" checksum="F2E5BA8B7102E40A" modified="2017-09-27" version="1">MSIFEVLKPLKTEIAKVHVFPPSEGEFGEFGFNNPEINALLEELGFTLYRHQVEALEKLYSGKNIVVTTPTASGKSEIFRLAIFDSYLSDPGRTYLLVYPTRALINNQFEKFSLQNLVFYRITGKSVSARILTGDVPWSERRELIREKPRVVFTTPDMLHYNVLRRWRDYEWLLRNLRYLVVDELHVYRGVFGSNAAWLFKRLLFRLKRLGVKPQIIALSATLRNPEEFAEKLFRARFEAVTEATNPFPRRYLVLFEPRNLEERQLLKALVERLAEKGIKTLVFFDSRKGTEKLMRFLLNSPAFSRTSTYKGTLPKNIRWEIERDFKDGKLLVLLTTNALELGIDIGDLDAVINYGIPPDGLFSLIQRFGRAGRKADREALNGIVLRKNGLDYYYREHFDELVERLEKGIIEYMPVKTDNERIAEKHLHYLLTELGILDWDELDEFERKIAERLVIERKADLKKNPLTGKLELRLRRPAFNYSSLRTASDESFFLVKDEPWIRGKLMEKASTRDLLNFINWLKMKGYVIEEVDTDEYHRSLLPGMAYFSRGELYMAMDRLSIGNFHFVFAGQLNRLWDVETFASKREDVEILEIKEEKSYRGVTIGLGKLRVRHVYTGYAVKGLDTGNYVAELVKLKERGILKGEIFSPASGEKVEAEEDFSILNWEKFAKVEFEEPHVREFETEGVWLVFPEWIRDVPAEEFREFLGKAAEKGFEDIAFHLYETLDRRKLFPLYLGSTSFIIRKMIGDALQKAGINDEELAFAIKKMVDSKDGIGSALHAIEHNLIKIAPIFTYVDSRELGGYSYASFPGEPYVGMPVVFIYDGNEGGAGLAEIIYGNIEKLMEKSLEHLKGCSCKDGCPVCVLSPKCGTFNEFLDKWSAIRVWERIFEEDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8S1</accession>
    <name>A0A218P8S1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CopG family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07525</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854430.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8S1"/>
    <dbReference type="GeneID" id="33316108"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07525"/>
    <dbReference type="OrthoDB" id="26043at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21631">
        <property type="entry name" value="RHH_CopG_NikR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="entry name" value="Met repressor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribbon-helix-helix protein CopG" evidence="1">
        <location>
            <begin position="5"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="86" mass="10225" checksum="4F9C1141D67FFD92" modified="2017-09-27" version="1">MGKVKTSVYLDEELWKEFKELAQREKSEVSKLLEEALMNYLINEVLKDVDDSEVPLWFEPLKVKGESSEKLVREMRDDREKRLLGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8U8</accession>
    <name>A0A218P8U8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mannose-6-phosphate isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07625</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8U8"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07625"/>
    <dbReference type="OrthoDB" id="301394at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02226">
        <property type="entry name" value="cupin_YdbB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052044">
        <property type="entry name" value="PKS_Associated_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36114">
        <property type="entry name" value="16.7 KDA PROTEIN IN WHIE LOCUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36114:SF1">
        <property type="entry name" value="16.7 KDA PROTEIN IN WHIE LOCUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-2" evidence="1">
        <location>
            <begin position="39"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="108" mass="12571" checksum="8FDB2ECF761E6E94" modified="2017-09-27" version="1">MIPKVSLDEKIREITEPWSPVEIARVNDYVVRMALFEGEYHWHKHTNEDELFYVYRGRIVIQLRGQPDITLEEGEMAVVAKGVEHCPKALEPSYVLMFEPAELKSRGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8V8</accession>
    <name>A0A218P8V8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_07720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854461.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8V8"/>
    <dbReference type="GeneID" id="33316146"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07720"/>
    <dbReference type="OrthoDB" id="371918at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041682">
        <property type="entry name" value="AAA_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025420">
        <property type="entry name" value="DUF4143"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33295">
        <property type="entry name" value="ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33295:SF21">
        <property type="entry name" value="ATPASE, AAA SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13173">
        <property type="entry name" value="AAA_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13635">
        <property type="entry name" value="DUF4143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="33"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4143" evidence="2">
        <location>
            <begin position="219"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="422" mass="48511" checksum="9B2B8EFAB3C2E9D2" modified="2017-09-27" version="1">MIGRSTWEEVLSDYLELRVEYTRRDVEVKLPKSRALAIVGPRRAGKTFFLFQLWDELDPERRRSLYVNFEDPRLVGATAEDLMEMLRSYYSLMSVPKGEKLLFLLDEVQVVEGWERFVRYVLDMGHKVILTGSSSKLLSKEIATVLRGRAITLNLYPFSLSEVLRVRGLEKALLGLETRGKLLGVVQECLEWGSYPEVVFQPELRREILREILDVTIYRDIVERWRVDNLKALRFLFKLLASSSHTSVTKLHSTMKSIGIAVGKPTLANYVEYLSDALVAFPLRAYVKSEKKKELLGFKPYFVDNGLLTVLGVSDRGRLLENLVFTELLKRGLRPNEDLFFYVTGSGREVDFIIPGEELIQVSWKLHPENRERELAALIEAASETGIREPTMVTWEKGKEEKIKLGGRVIKLLPVEEWVKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8W5</accession>
    <name>A0A218P8W5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Peptidase M54</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_07815</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854479.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8W5"/>
    <dbReference type="GeneID" id="33316167"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07815"/>
    <dbReference type="OrthoDB" id="50281at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11375">
        <property type="entry name" value="Peptidase_M54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012962">
        <property type="entry name" value="Pept_M54_archaemetzincn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15910">
        <property type="entry name" value="ARCHAEMETZINCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15910:SF1">
        <property type="entry name" value="ARCHAEMETZINCIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07998">
        <property type="entry name" value="Peptidase_M54"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="242" mass="28334" checksum="2D7629598B825AFA" modified="2017-09-27" version="1">MEFIAFTYVGNFMEKEVIDEVVFTVFDEANRFFHENDIPMRFLYIDKLKLEPGYLINLNTPEGKIRVYPLEALIDVLHARLLQEIEERPDIAMNKIFAITTFPLVSRNPYFDFYEKFLGIHETRLGLRIMVLSMKPFEPEGLNEMLKEMAGSENPNPRLKRNVREQLTLFKDRVLKGVLHEIGHGFGLEHCSNDCVMNSPSSMGEWDSRYPGYCDSCFINLKRAVEWSELTLRQKEGEKHQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Y5</accession>
    <name>A0A218P8Y5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleotidyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854450.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Y5"/>
    <dbReference type="GeneID" id="33316131"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07640"/>
    <dbReference type="OrthoDB" id="8895at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008832">
        <property type="term" value="F:dGTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006203">
        <property type="term" value="P:dGTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3210.10:FF:000037">
        <property type="entry name" value="Metal-dependent phosphohydrolase, HD superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050135">
        <property type="entry name" value="dGTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045509">
        <property type="entry name" value="HD_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373:SF4">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE SAMHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19276">
        <property type="entry name" value="HD_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="52"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="416" mass="47332" checksum="2D0CB32C03BA6555" modified="2017-09-27" version="1">MDGKIIHDGIHGSMKLTGLIFDLVKTPEFQRLRNIKQLGLAYLVYPGANHSRFEHSLGAWNIAKRLSSEVGLSDDESMLLQVGALLHDIGHGPFSHTFESIYQHYVKERDHMRLGQDIILGRINITESENGGRISEIIESYGYDFTPKDVADLILGKAEKPYLGQMLHGDVDVDQLDYLIRDAHYTGVAHGIIDLERLLKVLRIHDGELVVDEKGVEAVEGMMVARSLMYSRVYFHHTVKIAEGMLTRALEFALEEGHLWDFWRMVDCRVLVELEDLEGLPAEMVRRVKYRELYKAAVLASADGLSTEEKRELLAAYRNVKRRQEIERALADAVGAREGEVILEFSIADLMLSEPRLKSTEINVLLEDGEVQPLTKVTPLANALKRRQTPRWAVLIASPKEYVPKLREVWRRVIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Y6</accession>
    <name>A0A218P8Y6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Diadenylate cyclase</fullName>
            <shortName evidence="6">DAC</shortName>
            <ecNumber evidence="6">2.7.7.85</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Cyclic-di-AMP synthase</fullName>
            <shortName evidence="6">c-di-AMP synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">dacZ</name>
        <name evidence="8" type="ORF">A3L08_07800</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Diadenylate cyclase that catalyzes the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP). c-di-AMP is a second messenger for intracellular signal transduction involved in the control of important regulatory processes such as osmoregulation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>2 ATP = 3',3'-c-di-AMP + 2 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:35655"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:71500"/>
            <dbReference type="EC" id="2.7.7.85"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the adenylate cyclase family. DacZ subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.85" evidence="6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854476.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Y6"/>
    <dbReference type="GeneID" id="33316162"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07800"/>
    <dbReference type="OrthoDB" id="85944at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106408">
        <property type="term" value="F:diadenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1700.10:FF:000005">
        <property type="entry name" value="Diadenylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1700.10">
        <property type="entry name" value="DNA integrity scanning protein, DisA, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00840">
        <property type="entry name" value="DacZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014499">
        <property type="entry name" value="DAC_DacZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048542">
        <property type="entry name" value="DacZ_T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050338">
        <property type="entry name" value="DisA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036888">
        <property type="entry name" value="DNA_integrity_DisA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003390">
        <property type="entry name" value="DNA_integrity_scan_DisA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34185">
        <property type="entry name" value="DIADENYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34185:SF1">
        <property type="entry name" value="DIADENYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02457">
        <property type="entry name" value="DAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21756">
        <property type="entry name" value="DacZ_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019073">
        <property type="entry name" value="UCP019073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143597">
        <property type="entry name" value="YojJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51794">
        <property type="entry name" value="DAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAC" evidence="7">
        <location>
            <begin position="108"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="267" mass="29592" checksum="28E5F09BB9CB751A" modified="2017-09-27" version="1">MEKGIPSVLLESAVRIVKELGGKALVVLEDVKPEELPEVDVTVVIVGSSFDVENERVKRVSIPQNLDIHNVLNLISAFLIEHNILGEGDSFVYVTRQTIGIKTVKKSLSAMRGFFAQNQNVLQRLLEIAIELSIEGREGTPVGTIFVIGDTRRVLRHSHQLIPNPFKGHKVNVLDRDSKEIIKEFAQLDGAFVIRENGRITAAGRYLQVEPRVIDLMLPPGLGSRHIAAAGITKLTKAMAISLSESGTIRIFKNGLMLLEYNPRIKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Z4</accession>
    <name>A0A218P8Z4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bacterial Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07935</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854502.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Z4"/>
    <dbReference type="GeneID" id="33316192"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07935"/>
    <dbReference type="OrthoDB" id="94582at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046878">
        <property type="entry name" value="Big_14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20251">
        <property type="entry name" value="Big_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bacterial Ig-like" evidence="1">
        <location>
            <begin position="23"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="142" mass="16318" checksum="377A5468CD80C9E5" modified="2017-09-27" version="1">MRWKTSAAIVVLILLIWGLTGSHFYMGLDKESYHPGEEPVLTLRNTGILPIYFGQPYTLYRRENGTWVRVRQGFFFNSLLYDLLPFGSWKQEIALKYLPENESAGTVPTLYNLPPGEYRLVKEICGWPRGCVNGSVEFEILP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Z8</accession>
    <name>A0A218P8Z8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD-dependent protein deacylase</fullName>
            <ecNumber evidence="5">2.3.1.286</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Regulatory protein SIR2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cobB</name>
        <name evidence="8" type="ORF">A3L08_07910</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">NAD-dependent lysine deacetylase and desuccinylase that specifically removes acetyl and succinyl groups on target proteins. Modulates the activities of several proteins which are inactive in their acylated form. Deacetylates the N-terminal lysine residue of Alba, the major archaeal chromatin protein and that, in turn, increases Alba's DNA binding affinity, thereby repressing transcription.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N(6)-acetyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-acetyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:43636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="ChEBI" id="CHEBI:83767"/>
            <dbReference type="EC" id="2.3.1.286"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N(6)-succinyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-succinyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:11877"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:87830"/>
            <dbReference type="ChEBI" id="CHEBI:87832"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="5">2 residues (Tyr-64 and Arg-67) present in a large hydrophobic pocket are probably involved in substrate specificity. They are important for desuccinylation activity, but dispensable for deacetylation activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the sirtuin family. Class III subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.286" evidence="5"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854497.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Z8"/>
    <dbReference type="GeneID" id="33316187"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07910"/>
    <dbReference type="OrthoDB" id="728at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017136">
        <property type="term" value="F:histone deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036054">
        <property type="term" value="F:protein-malonyllysine demalonylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036055">
        <property type="term" value="F:protein-succinyllysine desuccinylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01412">
        <property type="entry name" value="SIRT5_Af1_CobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01121">
        <property type="entry name" value="Sirtuin_ClassIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027546">
        <property type="entry name" value="Sirtuin_class_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001753">
        <property type="entry name" value="PRK00481.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040867">
        <property type="entry name" value="prot_deacyl_CobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF10">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5 6">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="98"/>
            <end position="101"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="191"/>
            <end position="193"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="217"/>
            <end position="219"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="250" mass="28263" checksum="2A526D4277AD84C0" modified="2017-09-27" version="1">MIEEAAKLLARSRFAIAFTGAGISAESGVPTFRGFNGLWKKHRPEELATPEAFRKDPHLVWSFYRWRMGLIRNAKPNRAHYALAELEEMGILKAVITQNVDDLHREAGSRRIIELHGNIFRVRCTSCSYRENLKESGRLDEFLEEKDLPKCPECGSLLRPDVVWFGEPLPRKTLEEAFKLAERADLVLVIGTSGVVYPAAYIPQIVKETGGKVIEVNPEESGITPIADVFLRCPAGEAMEKLMERVRRLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P910</accession>
    <name>A0A218P910_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_07755</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854468.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P910"/>
    <dbReference type="GeneID" id="33316153"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07755"/>
    <dbReference type="OrthoDB" id="86058at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="23"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="125"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="ABC-2 type transporter transmembrane" evidence="6">
        <location>
            <begin position="49"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="234" mass="26959" checksum="1A42BDBC894413B6" modified="2017-09-27" version="1">MSFVKKFGAIYRTDIKLLRRDPMLLYSVAMTLVLLLIVRYFKDRVGVYYPLLALLTLIFIPMILGMIPGFMMADEKEDKTIQALQVIPISSEAFLAYRLTWASIVTALMTAGAPWILDIEMPQKGILALIALFLLEVWIYGLLITVFSESRMQALTVSKVLGWFLMLPPLIKLVVVWRNLSTDWSRFTAFLPTYWLYKVFDGIPANDYSDFPIAVMVHLLWLVPLVLLFRKRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P922</accession>
    <name>A0A218P922_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Type I restriction modification DNA specificity domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_08045</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-I restriction system S methylase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854521.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P922"/>
    <dbReference type="GeneID" id="33316214"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08045"/>
    <dbReference type="OrthoDB" id="84651at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.220.20">
        <property type="entry name" value="DNA methylase specificity domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000055">
        <property type="entry name" value="Restrct_endonuc_typeI_TRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044946">
        <property type="entry name" value="Restrct_endonuc_typeI_TRD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052021">
        <property type="entry name" value="Type-I_RS_S_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30408:SF12">
        <property type="entry name" value="TYPE I RESTRICTION ENZYME MJAVIII SPECIFICITY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30408">
        <property type="entry name" value="TYPE-1 RESTRICTION ENZYME ECOKI SPECIFICITY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01420">
        <property type="entry name" value="Methylase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116734">
        <property type="entry name" value="DNA methylase specificity domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0680">Restriction system</keyword>
    <feature type="domain" description="Type I restriction modification DNA specificity" evidence="4">
        <location>
            <begin position="69"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="473" mass="53345" checksum="E545E3FEF534ABD8" modified="2017-09-27" version="1">MRAISNISVVLNLQELKRNYRADPYSAHPKKVKGIEHLNQIAKEYGYDIVPLVELLKLPPSSGRSGLPLSQNGDVKIVKPANLTSLFFVDLTNCETTTEEAYNASEKGRLQNEDILVLSAAHAEGYIGTNTSIVRLDKNERAICIGELIRLRPDTSKINPYYLTLYLNSAIGKYLLNYSVRGQTVHLYPKDIEDLPVLLPPREIQDSIGNKLKEAIDKKIEAEEKRREIQEIFKQNLGDIDFEKLGGYTYKFSQVLEYERLDPHFYFPGFLKVLDLLKNSGLKIEKMSKLVEFSRETINPSEIDEFIYVEIADVDITYGFISSHSVVKGSNAPSRARKIIRKDALLIPLTRPYRGAIAVVDSRYDGAIATTGFSVSYPKPNSPVDSYYLCAFLKSKYGLIQLTQRMSNANYPAVLEKDISEILVPILSANDMRTISDKMKNIINNLLLSKQLHSQAMEELNRLLGLKSVGDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P950</accession>
    <name>A0A218P950_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glycerol-3-phosphate ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_08220</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854552.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P950"/>
    <dbReference type="GeneID" id="33316250"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08220"/>
    <dbReference type="OrthoDB" id="18368at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055052">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03301">
        <property type="entry name" value="ABC_MalK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000042">
        <property type="entry name" value="Maltose/maltodextrin ABC transporter, ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015855">
        <property type="entry name" value="ABC_transpr_MalK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047641">
        <property type="entry name" value="ABC_transpr_MalK/UgpC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040582">
        <property type="entry name" value="OB_MalK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008653">
        <property type="entry name" value="PRK11650.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43875">
        <property type="entry name" value="MALTODEXTRIN IMPORT ATP-BINDING PROTEIN MSMX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43875:SF1">
        <property type="entry name" value="OSMOPROTECTIVE COMPOUNDS UPTAKE ATP-BINDING PROTEIN GGTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17912">
        <property type="entry name" value="OB_MalK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="MOP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="4"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="372" mass="41446" checksum="9189A2DF55FAA3D2" modified="2017-09-27" version="1">MAGVRLENVWKIFGGFEAVKDMNLDIKDGEFMILLGPSGCGKTTTLRMIAGLEEPSKGQIYIGDKLVADPEKGVFVPPKDRDIAMVFQSYALYPHMTVYDNIAFPLKLRKVPKSEIDQRVREVAEMLGLSELLNRKPRELSGGQRQRVALGRAIVRRPKVFLMDEPLSNLDAKLRVKMRAELKRLQKQLGVTTIYVTHDQVEAMTMGDRIAVINQGQLQQVGTPEEVYDRPANTFVAGFIGSPPMNFMSASITEEGFADFGEFQLKLLPDQVEVLEEAGLIGKEVIFGIRPEDVYDAFFAQVKIPGENMVRALVEIMENLGGEKVVHLRVGDVALVGKFPAESKVSEGEEVDIVFDMKKIHIFEKGSGKAIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P996</accession>
    <name>A0A218P996_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nucleoside-diphosphate sugar epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_08465</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854599.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P996"/>
    <dbReference type="GeneID" id="33316299"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08465"/>
    <dbReference type="OrthoDB" id="4907at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd05256">
        <property type="entry name" value="UDP_AE_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="2">
        <location>
            <begin position="6"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="310" mass="34236" checksum="C5A583120BC872AD" modified="2017-09-27" version="1">MRNKLVVVTGGAGFIGSHIAWELVRDNDVIIIDSLYTGKEENVPPGAKLVKADIRDYEAIAELISNADYVFHEAAQVSVVESVRDPIFTEEVNVVGTLNILKALLEGHGKLIFASSAAVYGDNPNLPLKETERPRPLSPYGVTKATAEEYLRVFHELYGLPVVALRYFNVFGPRQSANQYAGVISIFINRALAGEPLVIFGDGKQTRDFIYVKDVVKANILAAESRKANGRVFNVATGKQTSILELATKVIEITGASTSIIFDKPRPGDIRHSLADISEIRKLGFEPEWPLEEGLKKTVEWYARTHSQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9D0</accession>
    <name>A0A218P9D0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08735</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854643.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9D0"/>
    <dbReference type="GeneID" id="33316353"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08735"/>
    <dbReference type="OrthoDB" id="101602at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.460">
        <property type="entry name" value="protein pf1176 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023108">
        <property type="entry name" value="DUF3216"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038317">
        <property type="entry name" value="Pf1176-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11505">
        <property type="entry name" value="DUF3216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DUF3216" evidence="1">
        <location>
            <begin position="8"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="100" mass="11649" checksum="7FB655105626FD57" modified="2017-09-27" version="1">MAVEAPEVEEVKKLLEELEEEALLARLESFVRLNEGLESKKGKEFIEVSILGFLEGILTVLRGKYPGKEDVEALYRKVKGRREELDEQFRKPRIPYLEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9G6</accession>
    <name>A0A218P9G6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854653.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9G6"/>
    <dbReference type="GeneID" id="33316364"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08790"/>
    <dbReference type="OrthoDB" id="102124at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="96" mass="10317" checksum="C42E7343DDDE94F8" modified="2017-09-27" version="1">MIGFVIGTVLGIAAALIYGRFKGKAGELTMAAMGIPLFTYLAAMGFYSGWDFAGGSVFFSTPLGNFTPNELIGLETFLATLVAFTYVWFAPRMPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9G8</accession>
    <name>A0A218P9G8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleotidyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_08700</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_157721610.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9G8"/>
    <dbReference type="GeneID" id="33316346"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08700"/>
    <dbReference type="OrthoDB" id="359531at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.620">
        <property type="entry name" value="JHP933, nucleotidyltransferase-like core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014942">
        <property type="entry name" value="AbiEii"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08843">
        <property type="entry name" value="AbiEii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="316" mass="37652" checksum="BCF1C948885ADFE6" modified="2017-09-27" version="1">MFPSDEFTGFLIRKTGIEKPLLVRRDALLHAILRELYGEERFHERYLFKGGTCLVKCYLGYYRFSVDLDFTFPLRDKLSRSERRKLISSEVRWLSEKLEYIAKGFGLNFRPFEGESYDRNFVHFEGNENRKIVFFHLFMPMGEVVKIEVNFFEPVLFEERRVQARTLLEGVKLENEEKAYFFEELEKYSTLPVTAYSPEEILAEKIRAILTRRIQKLRDFYDVFMLYKAGYDYSDVIDDAVEKIKFSMRFSPERTSENLRTNLKSIQSGRFNIAPELVENELELVLTRPPESEFLEFMNNFLEVLSEIDLSRIPRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9I1</accession>
    <name>A0A218P9I1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_08895</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854673.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9I1"/>
    <dbReference type="GeneID" id="33316385"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08895"/>
    <dbReference type="OrthoDB" id="44250at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050763">
        <property type="entry name" value="ABC_transporter_ATP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711:SF5">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN NATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="19"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="68" mass="7318" checksum="6FCDA17D01D619FE" modified="2017-09-27" version="1">MNVIEVENLTKYYGKLRALDSVSFSVPEGVILGVIGPNGAGKTTLIKILSCLLTYDEGTVRLFGKASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9J6</accession>
    <name>A0A218P9J6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_09015</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854693.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9J6"/>
    <dbReference type="GeneID" id="33316409"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09015"/>
    <dbReference type="OrthoDB" id="147058at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047817">
        <property type="entry name" value="ABC2_TM_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005942">
        <property type="entry name" value="Daunbcin-R_ABC-transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051784">
        <property type="entry name" value="Nod_factor_ABC_transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01247">
        <property type="entry name" value="drrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43229">
        <property type="entry name" value="NODULATION PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43229:SF2">
        <property type="entry name" value="NODULATION PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006648">
        <property type="entry name" value="DrrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00164">
        <property type="entry name" value="ABC2TRNSPORT"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="231"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-2" evidence="6">
        <location>
            <begin position="20"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="255" mass="27605" checksum="CAAFB57F0B401ECB" modified="2017-09-27" version="1">MRTFTTMIYRELKRFSRSRARVVGSLINPLIWLIFFGMGWSGVFNNPMAKSVFGGVDYLTYLVPGVFAMTVFNMSFMQGVTLILDKQFDFLKEILVAPASRAEAILGRITGGSLMALIQGTILLALSFALADLKLRGVLPALGLGFLVGIAIAGMGVAIALKMTSMEGFQMVVAMITMPMTFLSGAIYPIEAMPGWMKAIAYVNPLTYAVDGARRYLVGDAIAKFSPVVEWGVLALLALAFSALAALEFEKATID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9J7</accession>
    <name>A0A218P9J7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chromosome segregation protein ScpA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854701.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9J7"/>
    <dbReference type="GeneID" id="33316418"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09060"/>
    <dbReference type="OrthoDB" id="53244at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.580">
        <property type="entry name" value="Structural maintenance of chromosome 1. Chain E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003768">
        <property type="entry name" value="ScpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023093">
        <property type="entry name" value="ScpA-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33969">
        <property type="entry name" value="SEGREGATION AND CONDENSATION PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33969:SF2">
        <property type="entry name" value="SEGREGATION AND CONDENSATION PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02616">
        <property type="entry name" value="SMC_ScpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="111"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="220" mass="26014" checksum="0B0034793F38E2E2" modified="2017-09-27" version="1">MESRREEEITPVDILLQLVTMGKVDPWNIDIVDLTEKYIERLREMKELDLRVSARAILAASILVRMKSEALLHADDEEEEESPEESFHVDVEPLAPPLRRVERYYTFDDLLDALMDALEEAEKRKPRKKKKVEIEEEVFVVDDFRVDIEKHVYRLHEIVVEMYRESREPIRFWDLIFDPSPKIVARTFLYLLFLSNMGKVDLIQEEPFGEILVVPVEENA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9M3</accession>
    <name>A0A218P9M3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Geranylgeranylglyceryl phosphate synthase</fullName>
            <shortName evidence="10">GGGP synthase</shortName>
            <shortName evidence="10">GGGPS</shortName>
            <ecNumber evidence="10">2.5.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">(S)-3-O-geranylgeranylglyceryl phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Phosphoglycerol geranylgeranyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_09200</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P). This reaction is the first ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>sn-glycerol 1-phosphate + (2E,6E,10E)-geranylgeranyl diphosphate = sn-3-O-(geranylgeranyl)glycerol 1-phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23404"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57677"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="EC" id="2.5.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the GGGP/HepGP synthase family. Group II subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.41" evidence="10"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854726.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9M3"/>
    <dbReference type="GeneID" id="33316446"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09200"/>
    <dbReference type="OrthoDB" id="7409at2157"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047294">
        <property type="term" value="F:phosphoglycerol geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02812">
        <property type="entry name" value="PcrB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.390:FF:000001">
        <property type="entry name" value="Heptaprenylglyceryl phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.390">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00112">
        <property type="entry name" value="GGGP_HepGP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038597">
        <property type="entry name" value="GGGP/HepGP_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008205">
        <property type="entry name" value="GGGP_HepGP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010946">
        <property type="entry name" value="GGGP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050064">
        <property type="entry name" value="IGPS_HisA/HisF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01769">
        <property type="entry name" value="GGGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01768">
        <property type="entry name" value="GGGP-family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003198">
        <property type="entry name" value="PRK04169.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235:SF22">
        <property type="entry name" value="GERANYLGERANYLGLYCERYL PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE SUBUNIT HISF/H IGP SYNTHASE SUBUNIT HISF/H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01884">
        <property type="entry name" value="PcrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="175"/>
            <end position="181"/>
        </location>
        <ligand>
            <name>sn-glycerol 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="206"/>
            <end position="207"/>
        </location>
        <ligand>
            <name>sn-glycerol 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="228"/>
            <end position="229"/>
        </location>
        <ligand>
            <name>sn-glycerol 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ07482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="253" mass="26792" checksum="278EA168548339AF" modified="2017-09-27" version="1">MKPKIGKVESYIHEKLEREKLHFVLLDPDDVDPETAGKIAEMSEEIGVDAIMIGGSTGAEGEVLDGVVRAIKESSSLPTILFPGSHGGISKYADAIFFMSLLNSTNPFFITGAQALGAFQVKRYGIEPIPMAYLIVEPGETVGWVGDAKPIPRHKPKIAAAYALAGQYLGMRLVYLEAGSGAPQPVPPEMIAVVKKVIDVPLIVGGGIRTGEQARAAVKAGADIIVTGTAIEKAGSMDKAEEKLEELNRGIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9R9</accession>
    <name>A0A218P9R9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-binding protein A3L08_09465</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_09465</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the PDCD5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854773.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9R9"/>
    <dbReference type="GeneID" id="33316499"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09465"/>
    <dbReference type="OrthoDB" id="7912at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.140">
        <property type="entry name" value="PDCD5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00026">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022889">
        <property type="entry name" value="DNA_bind_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002836">
        <property type="entry name" value="PDCD5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036883">
        <property type="entry name" value="PDCD5-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003268">
        <property type="entry name" value="PRK04239.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840:SF0">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01984">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015730">
        <property type="entry name" value="TFAR19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46950">
        <property type="entry name" value="Double-stranded DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="13"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="112" mass="13498" checksum="52CD2FFD0D3BF4A6" modified="2017-09-27" version="1">MAEDIEEIRKKKLMELQKRYLEQQKAQEEAMRQEMELEAQLDAIMRRILTPEARERLGRVKLVKPELARQVQLVLVQLYQAGQIREPIDDAKLKRILAQIDERTRRDFNIKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9T5</accession>
    <name>A0A218P9T5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Carbon-nitrogen hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_09610</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854800.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9T5"/>
    <dbReference type="GeneID" id="33316528"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09610"/>
    <dbReference type="OrthoDB" id="41015at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07197">
        <property type="entry name" value="nitrilase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050345">
        <property type="entry name" value="Aliph_Amidase/BUP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43674:SF2">
        <property type="entry name" value="BETA-UREIDOPROPIONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43674">
        <property type="entry name" value="NITRILASE C965.09-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="223" mass="25562" checksum="B855822ECE002D02" modified="2017-09-27" version="1">MRVALIPMRVKVGDFDTNWAEFERRFDEALAHNPDFVVFPEYCLTGFEEWDFSGAELYDETVERVSSLARKNGVYVVFGLLEPYKNCVYNSALLIGRNGEVFLKHRKFQEPCKFCTGNTVRTAWTEFGKVAVIICGDLYNKRILKWVRRKRPAYLFVPMEYSPDYGQPNEEDIEAMAERVKLLGVRAFIVNSYPPGGAWVFDADGTLLASSEGDKTLLWEGQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9U2</accession>
    <name>A0A218P9U2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Methylmalonyl-CoA mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_09510</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854781.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9U2"/>
    <dbReference type="GeneID" id="33316508"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09510"/>
    <dbReference type="OrthoDB" id="38408at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004494">
        <property type="term" value="F:methylmalonyl-CoA mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03680">
        <property type="entry name" value="MM_CoA_mutase_ICM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.240:FF:000001">
        <property type="entry name" value="Probable methylmalonyl-coa mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.240">
        <property type="entry name" value="Methylmalonyl-CoA mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016176">
        <property type="entry name" value="Cbl-dep_enz_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006099">
        <property type="entry name" value="MeMalonylCoA_mutase_a/b_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006098">
        <property type="entry name" value="MMCoA_mutase_a_cat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00641">
        <property type="entry name" value="acid_CoA_mut_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48101:SF1">
        <property type="entry name" value="METHYLMALONYL-COA MUTASE, LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48101">
        <property type="entry name" value="METHYLMALONYL-COA MUTASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01642">
        <property type="entry name" value="MM_CoA_mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51703">
        <property type="entry name" value="Cobalamin (vitamin B12)-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="4" id="KW-0170">Cobalt</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methylmalonyl-CoA mutase alpha/beta chain catalytic" evidence="6">
        <location>
            <begin position="39"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="491"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="562" mass="64525" checksum="C250C0CD5CE815FA" modified="2017-09-27" version="1">MTFDKEKLAKIREEEKRWEETTVAKFLQKAPERKEKFMTDDGFEIKRVYTPADLGEDWDYMEKLGFPGEYPFTRGVYATMYRGRFWTMRQYAGFGTAEESNKRYKYLLEQGQTGLSVAFDLPTQIGYDSDHPMAEGEVGKVGVAIDSLWDMRILFDGIPLDKVSTSMTINSTAANLLAMYILVAEEQGVKPEQLRGTVQNDILKEYIARGTYIFPPQPSMRLTTDIIMYCAENVPKWNPISISGYHIREAGANAVQEVAFTLADGIEYVKAVIERGMDVDKFAGRLSFFFNAHNNFLEEIAKFRAARRLWAYIMKEWFNAKNPRSMLLRFHTQTAGSTLTAQQPENNIVRVAIQALAAVLGGTQSLHTNSYDEALSLPTEKSVRIALRTQQIIAYESGVVDTIDPLGGSYYIEWLTDHIYEEALKYIEKIQKMGGMMRAIERGYIQKEIAESAYKYQKEVEEKKRIIVGVNEFIVDEPLDVEILKVDPSIREKQIERLKKLRSERDSKKVEEALDRLRKAAESEDENLMPYIIEAHRHLATLGEVTDVLREVWGEYRAPLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9U9</accession>
    <name>A0A218P9U9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09625</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854803.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9U9"/>
    <dbReference type="GeneID" id="33316531"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09625"/>
    <dbReference type="OrthoDB" id="103575at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="173" mass="19942" checksum="E70F1C84F79F91DF" modified="2017-09-27" version="1">MTGRKALVVFVIALLLVASVFSTYSWWQCRKEKREILVDVYIGSQLSILALGEIGDLMEHQLQNNASKIVLLIYTMDYRDKAYEVGHTFLILYLHSDEDKFWKLSVAIRNLADFLSTALNGGPEECVHKLGENLETLKKFDALFKELRKYEDPFDIPAGLAEEFFNTSEQLKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9W8</accession>
    <name>A0A218P9W8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854783.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9W8"/>
    <dbReference type="GeneID" id="33316510"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09520"/>
    <dbReference type="OrthoDB" id="93707at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43471">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43471:SF10">
        <property type="entry name" value="SLL1107 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12679">
        <property type="entry name" value="ABC2_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="263" mass="29472" checksum="698F539B1704CA6D" modified="2017-09-27" version="1">MSAIGVLYSNEVMRLLRSRRLKVMLALMLLPVIVYFFTHEDITEYSTRALQISLQVNFASFIVNFWASVIGQLVVIILMSELIASEIDRGTIRLLLTKPIRKSEILFGKFFAGMTGMAVLFGVPYLTLQVYMVLLYKRGFEGFSSTVGDVFFALGVTLLVLGSLGAFSMFLSVILSRPLYASLASFGVIFVAQFILPQLPFFDDPERFTLNYQLGVLLKKGFTLHTGLDAYKGDPASSALFFAVTIILSLIFALIGLYRREYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Y8</accession>
    <name>A0A218P9Y8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cation:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00375</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Y8"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00375"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005133">
        <property type="entry name" value="PhaG_MnhG_YufB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01300">
        <property type="entry name" value="CPA3_mnhG_phaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009320">
        <property type="entry name" value="PRK12675.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34703">
        <property type="entry name" value="ANTIPORTER SUBUNIT MNHG2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34703:SF1">
        <property type="entry name" value="ANTIPORTER SUBUNIT MNHG2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03334">
        <property type="entry name" value="PhaG_MnhG_YufB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="91" mass="10020" checksum="7F7EC3ADB7B29B3E" modified="2017-09-27" version="1">MVFGALGIIRFPDVYTRLHAATKCDTGGAMSIILYLVLTMDAPALVRAKFLVLAFLIAMMNPMVSHAIARAAYRYGVIPKVVVDMYAWDNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Z1</accession>
    <name>A0A218P9Z1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lipoate--protein ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01635</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854859.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Z1"/>
    <dbReference type="GeneID" id="33314932"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01635"/>
    <dbReference type="OrthoDB" id="43646at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16443">
        <property type="entry name" value="LplA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050664">
        <property type="entry name" value="Octanoyltrans_LipM/LipL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43679:SF2">
        <property type="entry name" value="OCTANOYL-[GCVH]:PROTEIN N-OCTANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43679">
        <property type="entry name" value="OCTANOYLTRANSFERASE LIPM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21948">
        <property type="entry name" value="LplA-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="1">
        <location>
            <begin position="28"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="249" mass="27888" checksum="F73C6515521D4A4D" modified="2017-09-27" version="1">MRFIPLIVARPEVQMAIDEAIMRARIEGKVPDTVRLYAFSPSSVTIGRFQSVVHDVNLDEAKRLGIPVVRRITGGGSVFHDEFGEITYSVIAGEDLHPALKNVESSYRYLAGPLVDALNELGLDAGFSGLNDIVANGKKISGSAQTRRKGVILQHGTFMYSTRVDILGKVLRVSKAKLADKGVSSIWERVTTLEREGIKLNRWEAYELLKQSFFNAFPLEEGELTDYELELAEKLVEEKYGNPEWNEMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA03</accession>
    <name>A0A218PA03_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF211 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_02105</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854863.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA03"/>
    <dbReference type="GeneID" id="33315026"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02105"/>
    <dbReference type="OrthoDB" id="201945at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1340">
        <property type="entry name" value="MTH889-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003831">
        <property type="entry name" value="DUF211"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023129">
        <property type="entry name" value="MTH889-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42240">
        <property type="entry name" value="DUF211 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42240:SF1">
        <property type="entry name" value="DUF211 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02680">
        <property type="entry name" value="DUF211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160363">
        <property type="entry name" value="MTH889-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="101" mass="11188" checksum="6C6EC1E636D77B1C" modified="2017-09-27" version="1">MAKGIRLLVLDVLKPHQPMVTELALGLSELEGVDGVNITLVEIDKETENVKITIVGDNLDYEEIVRTIEEFGGVVHSIDMVAAGRRIVEEGETPQDKLEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA26</accession>
    <name>A0A218PA26_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF373 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA26"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03690"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007254">
        <property type="entry name" value="DUF373"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38815:SF1">
        <property type="entry name" value="DUF373 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38815">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED, DUF373 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04123">
        <property type="entry name" value="DUF373"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="300"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="374" mass="40874" checksum="0D35DA687585D6B6" modified="2017-09-27" version="1">MKALVLAIDRDDDFGQKAGVEGPVIGRDACVDAALKLSLADPEDSDANVVYAAVKLHDELKESGEFDEVEVALITGHPKVGLKSDMELARQLEEVLKVFPADGVIPVTDGAEDEQIFPIITSRVPILTSHRVVVKQSEGIETTYYIIYRYMKEILSDPEVAKVVLGIPGMILLLYGIARLISVWYPESVKIISATITGTILLLIGGYFFTKGFRFNVRETVTKQFVVVISVIAGSLIIAGGAINAYFRLEEYALELIGSYPGTPLLATLIYISALNASLILGVSVMITGKVIQSYLKKDYHIWYQSSALLMMPAMWVTIDLTTRYAMAILTLSDIEVFTKLLLAIMDVAVAILVGVYMRGKVKGWEKVEAGASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA67</accession>
    <name>A0A218PA67_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Methylmalonyl-CoA epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_03910</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA67"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03910"/>
    <dbReference type="OrthoDB" id="86233at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015081">
        <property type="term" value="F:sodium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014497">
        <property type="entry name" value="MeMalonyl-CoA_deCOase_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005899">
        <property type="entry name" value="Na_pump_deCOase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01195">
        <property type="entry name" value="oadG_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04277">
        <property type="entry name" value="OAD_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018867">
        <property type="entry name" value="Na_decarb_PF0672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="42"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="125" mass="13817" checksum="8E5D4DACC886DBF1" modified="2017-09-27" version="1">MAEFMEGLNLTVLGVTIVFTVLAILAVVLYAVGWAERRLVEKEAKTAPSPASTPAPAPEAKEEKPAIPPRDLAVITAAVLAYTAEKASQLRPLPFKRKISDSWRLYGLQTQMEDVEDFNYEIGKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA75</accession>
    <name>A0A218PA75_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 13 14">Ribonuclease HII</fullName>
            <shortName evidence="13">RNase HII</shortName>
            <ecNumber evidence="6 13">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">rnhB</name>
        <name evidence="18" type="ORF">A3L08_07305</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 13 16">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13 16">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13">Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 13 16">Belongs to the RNase HII family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13 15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="6 13"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA75"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07305"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032299">
        <property type="term" value="C:ribonuclease H2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07180">
        <property type="entry name" value="RNase_HII_archaea_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.460:FF:000001">
        <property type="entry name" value="Ribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.460">
        <property type="entry name" value="Ribonuclease hii. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00052_A">
        <property type="entry name" value="RNase_HII_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004649">
        <property type="entry name" value="RNase_H2_suA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001352">
        <property type="entry name" value="RNase_HII/HIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024567">
        <property type="entry name" value="RNase_HII/HIII_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020787">
        <property type="entry name" value="RNase_HII_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023160">
        <property type="entry name" value="RNase_HII_hlx-loop-hlx_cap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00729">
        <property type="entry name" value="ribonuclease HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954:SF23">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01351">
        <property type="entry name" value="RNase_HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51975">
        <property type="entry name" value="RNASE_H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 13 16" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="12 13 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13 16" id="KW-0540">Nuclease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-2" evidence="17">
        <location>
            <begin position="1"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="NCBIfam" id="TIGR00729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ASJ07672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="212" mass="24646" checksum="6B4D930FB7616B40" modified="2017-09-27" version="1">MVIAAVVVDEENVPKLAELGVRDSKRLTPKRRERLFDEIIKLLDDYAVIELWPNEIDSREGTLNEFEVENFVKALNSLKVRPDVIYIDAADVKEERFGEDIGKGLNFRAEIVAEHKADDKFVPVSAASILAKVTRDRAIERLKEEYGEIGSGYPSDPRTRAFLEEYYRKHGDFPPIVRRSWKTLRKIEEKLKDEVRPKEKKREQTSLEDFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P500</accession>
    <name>A0A218P500_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NUDIX hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_00110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853110.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P500"/>
    <dbReference type="GeneID" id="33314626"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00110"/>
    <dbReference type="OrthoDB" id="40462at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18873">
        <property type="entry name" value="NUDIX_NadM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43736">
        <property type="entry name" value="ADP-RIBOSE PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43736:SF1">
        <property type="entry name" value="DIHYDRONEOPTERIN TRIPHOSPHATE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="37"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ05847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="169" mass="19077" checksum="AF8D41FA1BF78D77" modified="2017-09-27" version="1">MDRYVLLLKTPKDYDVSPLREELKEFLTERHPEIRAELHRCIGLTADVVILYNGGVVLIKRKHEPFKDHYALPGGFVEYGERVEEAAVREAKEETGLDVRLLRLVGVYSDPGRDPRGHTVTTAFLALGTGELKAGDDAKEVHVVPLEEALKLPLAFDHEKILRDALTLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P501</accession>
    <name>A0A218P501_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853112.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P501"/>
    <dbReference type="GeneID" id="33314628"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00120"/>
    <dbReference type="OrthoDB" id="86095at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018646">
        <property type="entry name" value="12TM_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054100">
        <property type="entry name" value="12TM_1_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09847">
        <property type="entry name" value="12TM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018875">
        <property type="entry name" value="UCP018875_ABC_perm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="306"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="334"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="376"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="407"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="442"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="469"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="487" mass="52747" checksum="8FAE1A9C4D278BEB" modified="2017-09-27" version="1">MLGIVRILYRELHYRRLKNNPQIAADPKKFRKQLRNAGDIKRGVALQSVAFLFFGLMMAGAIVGAEDDTRAAVLLATYALLPFVMALYTTTVNASYAVSMGIFEPLKPLPIKTGAKYLSVLLAIDNVPAIVALIPAVLAMAYRSPVSGLMGFLWILLGAFLGHVLGLVVFTLFGSSSVGGRFSKLRTMARVLGAILFIGMFYAINYVQMYVSEHYEELLPFFSRYSVAYPFSIASILEPLHSFLLVLGYLAVLVPTYRFILGKLWGRMEEGAAVSHVGTVSFKARTHHPVIAIALKDLRIAVRKSALLVGLIFPLFVVLPSALGVLTSGEMGEWSVASVLLMIAWMASVGVDTVLKIDGREFEFLRSLPLTLGQFLRAKLLVMNAVPVTAGAGLVLAAAYIDPGALKLLPAALILPFLTSSIALAFFYHGEKELSVPETNTGHVLILIILNGITLGAVAGLWYALGYPYAMLLAGLGIAVVLRVLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P512</accession>
    <name>A0A218P512_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_00250</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC-2 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853137.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P512"/>
    <dbReference type="GeneID" id="33314654"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00250"/>
    <dbReference type="OrthoDB" id="101939at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1710.10">
        <property type="entry name" value="abc type-2 transporter like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051449">
        <property type="entry name" value="ABC-2_transporter_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047817">
        <property type="entry name" value="ABC2_TM_bact-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30294">
        <property type="entry name" value="MEMBRANE COMPONENT OF ABC TRANSPORTER YHHJ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30294:SF29">
        <property type="entry name" value="MULTIDRUG ABC TRANSPORTER PERMEASE YBHS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="297"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="388"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-2" evidence="9">
        <location>
            <begin position="164"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ05874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="423" mass="45447" checksum="D52D77E9341E2508" modified="2017-09-27" version="1">MSDFWVLARKEIMNLIRDKKLLFGLVIVPLIIYPAMGKMIQTGLQQAQEETQVAVINLDEGNYGEVLIKALEVTPNVSVTVLEAGSIEEALEKASEEGQNVLVVIPRNFSQSIEGNTIATLQIYGIFRSVGTGIRESVSESRINAVVGVLSEQIARIKIEALGVKNPEAVLHPISVTSMSVIKGRIVPVSPSLVSGVLASQAYSLPLIIFLMVTITAQMAAGAVAAEKENKTLETLLTLPVKRTTIVASKISGTAVMGLIAALAYMVGLRSYMGTFMVETGVTLQDLGLQTTPTGMVLFAVVVFLTIVFALSLAMLLAVFAEDVQSANTVVSSVILPLAFPAFILMFTDISELPTFARYALMAVPFTHPILSYRYVLGGDYTSLGHSIAYLAVIAAVTLYLTARVFSGERILTAKISWGKKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P532</accession>
    <name>A0A218P532_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Signal recognition particle</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_00310</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853148.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P532"/>
    <dbReference type="GeneID" id="33314666"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00310"/>
    <dbReference type="OrthoDB" id="56356at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.30">
        <property type="entry name" value="Signal recognition particle, SRP19-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002778">
        <property type="entry name" value="Signal_recog_particle_SRP19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036521">
        <property type="entry name" value="SRP19-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002993">
        <property type="entry name" value="PRK03745.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01922">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69695">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0733">Signal recognition particle</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ05885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="114" mass="12957" checksum="35CC898F4ACBD910" modified="2017-09-27" version="1">MRKFVVWPNELDARLSRRYGRAVGKEFAVDSPGVQEIADAAVALGMKVIEVDAEKMNPRLAGLDEEYRLKGMLRIESKHPKGKSLKMLGQKIREIRKTQVKSKGKKHKSGKKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P536</accession>
    <name>A0A218P536_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pyruvoyl-dependent arginine decarboxylase</fullName>
            <shortName evidence="6">PvlArgDC</shortName>
            <ecNumber evidence="6">4.1.1.19</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="6">Pyruvoyl-dependent arginine decarboxylase subunit beta</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="6">Pyruvoyl-dependent arginine decarboxylase subunit alpha</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="6" type="primary">pdaD</name>
        <name evidence="7" type="ORF">A3L08_00415</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>L-arginine + H(+) = agmatine + CO2</text>
            <dbReference type="Rhea" id="RHEA:17641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:58145"/>
            <dbReference type="EC" id="4.1.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="6">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the PdaD family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.19" evidence="6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853167.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P536"/>
    <dbReference type="GeneID" id="33314687"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00415"/>
    <dbReference type="OrthoDB" id="30748at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008792">
        <property type="term" value="F:arginine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006527">
        <property type="term" value="P:L-arginine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.20.10">
        <property type="entry name" value="Pyruvoyl-Dependent Histidine Decarboxylase, subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01404">
        <property type="entry name" value="PvlArgDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016104">
        <property type="entry name" value="Pyr-dep_his/arg-deCO2ase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016105">
        <property type="entry name" value="Pyr-dep_his/arg-deCO2ase_sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002724">
        <property type="entry name" value="Pyruvoyl-dep_arg_deCO2ase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00286">
        <property type="entry name" value="pyruvoyl-dependent arginine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40438">
        <property type="entry name" value="PYRUVOYL-DEPENDENT ARGININE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40438:SF1">
        <property type="entry name" value="PYRUVOYL-DEPENDENT ARGININE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01862">
        <property type="entry name" value="PvlArgDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005216">
        <property type="entry name" value="Pyruvoyl-dep_arg_deCO2ase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00471">
        <property type="entry name" value="Pyruvoyl-dependent_arginine_de"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01170">
        <property type="entry name" value="Pyruvoyl-dependent_arginine_de"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00055">
        <property type="entry name" value="Pyruvoyl-Dependent_Histidine/A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56271">
        <property type="entry name" value="Pyruvoyl-dependent histidine and arginine decarboxylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 6" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_5023408851" description="Pyruvoyl-dependent arginine decarboxylase subunit beta" evidence="6">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023408852" description="Pyruvoyl-dependent arginine decarboxylase subunit alpha" evidence="6">
        <location>
            <begin position="44"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic)" evidence="6">
        <location>
            <begin position="43"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser)" evidence="6">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ05904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="157" mass="16981" checksum="5EE20219D0EA8505" modified="2017-09-27" version="1">MSWTTPKRAFMGAATAEGGTKLNAFDNALLKLGIGNVNLVKLSSVIPAHIEWMNEVHDVPIGMLLPTVYAHIESDEPGMTISAALGVGISENNEGGLIYEYSGYCTKEEAEEMVRKMVEEGFAQRGWKLAEFRVASASITVKDKPAAALAVVVMFPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P548</accession>
    <name>A0A218P548_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">acetate--CoA ligase (ADP-forming)</fullName>
            <ecNumber evidence="2">6.2.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_00460</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP + CoA = acetyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15081"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="6.2.1.13" evidence="2"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853176.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P548"/>
    <dbReference type="GeneID" id="33314696"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00460"/>
    <dbReference type="OrthoDB" id="18103at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043758">
        <property type="term" value="F:acetate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051538">
        <property type="entry name" value="Acyl-CoA_Synth/Transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043938">
        <property type="entry name" value="Ligase_CoA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032875">
        <property type="entry name" value="Succ_CoA_lig_flav_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43334">
        <property type="entry name" value="ACETATE--COA LIGASE [ADP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43334:SF2">
        <property type="entry name" value="ACETATE--COA LIGASE [ADP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19045">
        <property type="entry name" value="Ligase_CoA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13607">
        <property type="entry name" value="Succ_CoA_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA-binding" evidence="6">
        <location>
            <begin position="6"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ05913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="458" mass="49622" checksum="791F3AA0F45A2703" modified="2017-09-27" version="1">MTFDYFFKPKAIAVIGASNDPLKLGYEVFKNLKKYSGGKVYPVNVKDEEVQGVKAYKNVKDIPDEVDLAVVVVPKRFVKQTIIDCGEKGVKGIILITAGFGEVGEEGKREERELVEIAHRYGMRIVGPNCVGIMNTHNDMNATFVMDAKKGDIAFISQSGALGAGIIYKTVKEGIGFSKFVSIGNMADVDFAEFMEYLADTEEDKAIALYIEGLKDGRKFMEVAKRVTQKKPVIVLKAGKSESGARAASSHTGSLAGSYKIYEAAFKQSGVIVAETIDDMLSMARAFTQPLPKGKRVAIMTNAGGPGVLTADAIDKLGLKLADLEEKTIEELRSFLPPMAAVKNPVDMIASARGEDYYRTAKALLQDENVDMLISICVVPTFAGMTPTEHAEGVVKAVKEVNNGKPVLGLFMAGYVSEKAKELLEKNGIPSYERPEDVAAGAYALVEFAKARGVLKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P575</accession>
    <name>A0A218P575_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0173 metal-dependent hydrolase A3L08_00450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_00450</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0173 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853174.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P575"/>
    <dbReference type="GeneID" id="33314694"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00450"/>
    <dbReference type="OrthoDB" id="28313at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00457">
        <property type="entry name" value="UPF0173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022877">
        <property type="entry name" value="UPF0173"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050114">
        <property type="entry name" value="UPF0173_UPF0282_UlaG_hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001911">
        <property type="entry name" value="PRK00685.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43546:SF3">
        <property type="entry name" value="UPF0173 METAL-DEPENDENT HYDROLASE MJ1163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43546">
        <property type="entry name" value="UPF0173 METAL-DEPENDENT HYDROLASE MJ1163-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13483">
        <property type="entry name" value="Lactamase_B_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="3">
        <location>
            <begin position="8"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ05911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="224" mass="24235" checksum="CE7E5D88FA3A8BAB" modified="2017-09-27" version="1">MVKVKFLGHAAFLIEGSKKILVDPFLTGNPKAAAKPEELEADLILVTHAHGDHIGDAVAIAQRTGAKIVAMYDVANYLVENNSGITTIGMNYGPTKVDGVKIVQVPAWHSSSDGKYSIGNACGYIIEIDGVKIYHAGDTFVFSDMALFNELYGPIDVALLPIGGHFTMGPREAAKAVELLKPRKVIPMHYNTWPPIAQDPEEFKRLVGDKAEVVILEPGESLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P582</accession>
    <name>A0A218P582_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Adhesin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853201.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P582"/>
    <dbReference type="GeneID" id="33314724"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00600"/>
    <dbReference type="OrthoDB" id="86202at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="238" mass="25190" checksum="EA7DD867BE90FB9B" modified="2017-09-27" version="1">MLRRGGVLPIIAGVVLAVILIGAVAAAVIVHKYQTHRKEAGPLKELGAFDAGGVVIRDTVGDVRIVRANVSGVVLKSNLPVKANYSGGLLTVYCPTERKRGLLRFGEHNACSDYRDGKVVIEVGRKLADIWVKDTVGDVEVDVNATRLVLKDVVGDVRASAPAEYLVEDIVGDVSIDAEGDVEVSDVVGDVYISIPANFSVQLSVKDVVGDVENTHSGEGMPVLVRVSDVVGDVRIGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5A0</accession>
    <name>A0A218P5A0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Gamma-glutamyl-gamma-aminobutyrate hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853194.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5A0"/>
    <dbReference type="GeneID" id="33314716"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00560"/>
    <dbReference type="OrthoDB" id="3321at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01745">
        <property type="entry name" value="GATase1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011697">
        <property type="entry name" value="Peptidase_C26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044668">
        <property type="entry name" value="PuuD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43235">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE PB2B2.05-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43235:SF1">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE PB2B2.05-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07722">
        <property type="entry name" value="Peptidase_C26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="295" mass="33131" checksum="7CED9C06F9F2E696" modified="2017-09-27" version="1">MKPLIGIIGQMNHSKNELFLDRTHIEKIAEAGGIPAVFNTDASPEEVLEHVDGILLIEGPDVHPYFYGEDPSSAIKFVDTHRDEFEIKLVRAAVERGIPIFGIGRGMQVINVALGGTLYQDISEIPKAIKHDWELKLIGPSQRVHGVRIKMSSKLYGILKDGLDIEGTNEVYVNVNSFHHQAVKRVGEGLKPAAYSVDGLIEAIESAEETETFIIGVQWQPEYLPEMGRLYKAFVEAASEYRARKFELERRKIEVKLREELVEPREPEEEGPTAEPDESHHSLETTDSLHDTTQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5B5</accession>
    <name>A0A218P5B5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ferredoxin-NADP reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_00765</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="1">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853229.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5B5"/>
    <dbReference type="GeneID" id="33314757"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00765"/>
    <dbReference type="OrthoDB" id="35401at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06219">
        <property type="entry name" value="DHOD_e_trans_like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012165">
        <property type="entry name" value="Cyt_c3_hydrogenase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019480">
        <property type="entry name" value="Dihydroorotate_DH_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050353">
        <property type="entry name" value="PyrK_electron_transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004862">
        <property type="entry name" value="PRK06222.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43513">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE B (NAD(+)), ELECTRON TRANSFER SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43513:SF3">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE B (NAD(+)), ELECTRON TRANSFER SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10418">
        <property type="entry name" value="DHODB_Fe-S_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006816">
        <property type="entry name" value="Cyc3_hyd_g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR006816-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ05967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="282" mass="31781" checksum="8B7D96A4CBB2CCF3" modified="2017-09-27" version="1">MGYKITKKENLSAIDFFMEVEAPHVARSWKPGQFVVLIVDEKGERVPMSVYYAEDGKIGMFIRRHGVTTFKLWYEKQVGDELLSVAGPLGKPIEVKYYGNVVFASDAVCAQAESYATLKAMKEAGNYTIAIQSFENKDNVYPEKFLAKPVADEHYLTTDDGSVGIKGHYLDVLKELIEKDKVDIVFAGGKLGSLKKLAELTRPYGIPTIVTVRQIMVDGTGMCGSCRILYDGQIKFACRDGPMFDAHKVDWDDVIKRDARFVEQQNLARERYLQKLREKGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5C2</accession>
    <name>A0A218P5C2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853248.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5C2"/>
    <dbReference type="GeneID" id="33314778"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00870"/>
    <dbReference type="OrthoDB" id="40991at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="entry name" value="Phosphate transport system protein phou homolog 2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PHOSPHATE-SPECIFIC TRANSPORT SYSTEM ACCESSORY PROTEIN PHOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930:SF2">
        <property type="entry name" value="PHOU DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109755">
        <property type="entry name" value="PhoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="8"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="304" mass="35293" checksum="B16EF89E9FF4E0DE" modified="2017-09-27" version="1">MEFRKIQFTGRSSYIISLPKKWVREHNLSQGDVVPLSINPDGSITIFPKEPREVSEKKILSISREYSPDMAVRLVISAYIQGYDVLEINLSEEMPLYKVKIRKVLQSLPGVEIILDEPQRIVAKSLLDEEEINLAELLSRIRSLVMSMLGDLELLIASPDDREILRDINDLENELDRFYFLIIRAVNRLLSKRGVTEESGIIRRTFDLLGILFIVRNIERIGDHITRIAENPSEINVPYLKEKFGEMMAQIEERDLSKIDRLMLELKEEIRSIDYRQSIALESYRRVLEYLENIGETIINMALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5C8</accession>
    <name>A0A218P5C8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_00815</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853237.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5C8"/>
    <dbReference type="GeneID" id="33314767"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00815"/>
    <dbReference type="OrthoDB" id="78194at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019277">
        <property type="entry name" value="DUF2304"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10066">
        <property type="entry name" value="DUF2304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="89"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ05975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="132" mass="15062" checksum="6AEDE7DA46B301C8" modified="2017-09-27" version="1">MYAVQYIAIVVVAALMLYVLGKYGKKEFEWGDFLFWESILLGMLVVSVFPVQIANEIRKLLGLGRGLDALFVIAIGIAYLMVFKVYLAVDRTEREITELTRKVAIELEELTRKLDELQKGLETNEKTGKKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5C9</accession>
    <name>A0A218P5C9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_00735</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853223.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5C9"/>
    <dbReference type="GeneID" id="33314751"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00735"/>
    <dbReference type="OrthoDB" id="5583at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001080">
        <property type="entry name" value="3Fe4S_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051269">
        <property type="entry name" value="Fe-S_cluster_ET"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36923">
        <property type="entry name" value="FERREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36923:SF3">
        <property type="entry name" value="FERREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13459">
        <property type="entry name" value="Fer4_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00352">
        <property type="entry name" value="3FE4SFRDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="6 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="3"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ05961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="63" mass="6585" checksum="8100D3F41C7B3990" modified="2017-09-27" version="1">MAWKVTVDQDTCIGDAICASLCPDVFEMGDDGKAHPIVETTDLDCAQEAAEACPVGAITLEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5D4</accession>
    <name>A0A218P5D4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA-splicing endonuclease</fullName>
            <ecNumber evidence="4">4.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">tRNA-intron endonuclease</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">endA</name>
        <name evidence="7" type="ORF">A3L08_00990</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.</text>
            <dbReference type="EC" id="4.6.1.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.16" evidence="4"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853268.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5D4"/>
    <dbReference type="GeneID" id="33314802"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00990"/>
    <dbReference type="OrthoDB" id="46045at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22363">
        <property type="entry name" value="tRNA-intron_lyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1350.10:FF:000006">
        <property type="entry name" value="tRNA-splicing endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.20">
        <property type="entry name" value="tRNA intron endonuclease, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01833">
        <property type="entry name" value="EndA_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006677">
        <property type="entry name" value="tRNA_intron_Endonuc_cat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006678">
        <property type="entry name" value="tRNA_intron_Endonuc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036740">
        <property type="entry name" value="tRNA_intron_Endonuc_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006676">
        <property type="entry name" value="tRNA_splic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016442">
        <property type="entry name" value="tRNA_splic_arch_short"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00324">
        <property type="entry name" value="endA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21227">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21227:SF0">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01974">
        <property type="entry name" value="tRNA_int_endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02778">
        <property type="entry name" value="tRNA_int_endo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005285">
        <property type="entry name" value="tRNA_splic_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="tRNA-intron endonuclease catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55267">
        <property type="entry name" value="tRNA-intron endonuclease N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA intron endonuclease N-terminal" evidence="6">
        <location>
            <begin position="7"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA intron endonuclease catalytic" evidence="5">
        <location>
            <begin position="80"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="171" mass="20298" checksum="9E1D498CE24017D4" modified="2017-09-27" version="1">MKEPIVFQLSGDRVFSEREKAINQFYNKRYFGEVVNGKLFLSLIEAAYLMEKGKIRVFDGDRELSFQDLVELGRKRDDQFDIKLLVYTDLRDRGYTVKSALKFGSHFRVYRRGMDEHSQWLVWVVPENLRFSPNDITARVRVAHGVRKNMVMAVVDEDNDVVYYKIEWVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5D5</accession>
    <name>A0A218P5D5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Plant-type L-asparaginase</fullName>
            <ecNumber evidence="1">3.5.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">L-asparagine amidohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_00865</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-asparagine + H2O = L-aspartate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21016"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="EC" id="3.5.1.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.5.1.1" evidence="1"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853247.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5D5"/>
    <dbReference type="GeneID" id="33314777"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00865"/>
    <dbReference type="OrthoDB" id="18230at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04512">
        <property type="entry name" value="Ntn_Asparaginase_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.30:FF:000001">
        <property type="entry name" value="Isoaspartyl peptidase/L-asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.30">
        <property type="entry name" value="(Glycosyl)asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000246">
        <property type="entry name" value="Peptidase_T2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188:SF6">
        <property type="entry name" value="N(4)-(BETA-N-ACETYLGLUCOSAMINYL)-L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01112">
        <property type="entry name" value="Asparaginase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="203"/>
            <end position="206"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="225"/>
            <end position="228"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="10">
        <location>
            <begin position="175"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ05985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="306" mass="32741" checksum="546BA7523E16046F" modified="2017-09-27" version="1">MVAIIVHGGAGTIRKEERIPKVIEGVREAVLAGWRELKRGSALDAVEEAVKALEDNPLFNAGTGSVLTLDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLIGEGAVKFARLLGFEEYDPRTEERLKQWEELRKKLVEKGETRHWKKLNELIKEYPEVLRSTVGAVAFDGEEVVAGTSTGGVFLKMFGRVGDTPIIGGGTYANEVAGASCTGLGEVAIKLALAKSATDFVRLGMDAGAASEAAISLATRYFGPDTMGIIMVDSRGNVGFAKNTKHMSYAFMKDGMNEPEAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5F9</accession>
    <name>A0A218P5F9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphatidate cytidylyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853253.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5F9"/>
    <dbReference type="GeneID" id="33314784"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00900"/>
    <dbReference type="OrthoDB" id="100386at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="22786" checksum="26AFBB730B8D3C9E" modified="2017-09-27" version="1">MINTSWAVTIGVLFIIGTIAFTKKIGPEWAWINRKLIHLSVVPAILMFYWGFIPRWVFSGAAFLIGLIQLWPHLRKRELSWYQIGHNYGEVFFALSASVISATLPIEYATALLLAMAISDGVTGIIRHYYFRKNGFNVKLKKHWTGSLGYFVTAVIIAFIFLNAGTVEKIGWAAMLTLAEYQRLLDDNLAVPIVGGLLFLLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5G2</accession>
    <name>A0A218P5G2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Aspartate carbamoyltransferase regulatory chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pyrI</name>
        <name evidence="10" type="ORF">A3L08_01085</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Involved in allosteric regulation of aspartate carbamoyltransferase.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Contains catalytic and regulatory chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the PyrI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853286.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5G2"/>
    <dbReference type="GeneID" id="33314821"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01085"/>
    <dbReference type="OrthoDB" id="7000at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009347">
        <property type="term" value="C:aspartate carbamoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.20">
        <property type="entry name" value="Aspartate carbamoyltransferase regulatory subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.140">
        <property type="entry name" value="Aspartate carbamoyltransferase regulatory subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00002">
        <property type="entry name" value="Asp_carb_tr_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020545">
        <property type="entry name" value="Asp_carbamoyltransf_reg_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002801">
        <property type="entry name" value="Asp_carbamoylTrfase_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020542">
        <property type="entry name" value="Asp_carbamoyltrfase_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036792">
        <property type="entry name" value="Asp_carbatrfase_reg_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036793">
        <property type="entry name" value="Asp_carbatrfase_reg_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00240">
        <property type="entry name" value="ATCase_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35805">
        <property type="entry name" value="ASPARTATE CARBAMOYLTRANSFERASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35805:SF1">
        <property type="entry name" value="ASPARTATE CARBAMOYLTRANSFERASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01948">
        <property type="entry name" value="PyrI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02748">
        <property type="entry name" value="PyrI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57825">
        <property type="entry name" value="Aspartate carbamoyltransferase, Regulatory-chain, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54893">
        <property type="entry name" value="Aspartate carbamoyltransferase, Regulatory-chain, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aspartate carbamoyltransferase regulatory subunit N-terminal" evidence="8">
        <location>
            <begin position="4"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartate carbamoyltransferase regulatory subunit C-terminal" evidence="9">
        <location>
            <begin position="100"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="151" mass="16796" checksum="D0162AD3B39B90B9" modified="2017-09-27" version="1">MSELKVTAIKEGTVIDHIPAGKGLKVIEILRLNRVNGGVLLLAANVSSGKLGRKDIVKVEGKFLSEEEVNKIALIAPNATVNIVRDYRVAEKFRVSIPDEIVGILRCPNPNCVSNHEYVRSRFHVEGREPLKLRCHYCERTIEGDEILGNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5H7</accession>
    <name>A0A218P5H7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01275</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853319.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5H7"/>
    <dbReference type="GeneID" id="33314860"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01275"/>
    <dbReference type="OrthoDB" id="98089at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="84" mass="9485" checksum="A50200FF0A88CF13" modified="2017-09-27" version="1">MEKSEQNLYEIFGEVIAEQDFLKAFLITSALAFLLYFSAPQIVRMMGREDLLNALRVTLSALGAFLGFIVSTVIIEPKRVVEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5K8</accession>
    <name>A0A218P5K8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pyruvate ferredoxin oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_01335</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of one alpha, one beta, one delta and one gamma chain.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853331.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5K8"/>
    <dbReference type="GeneID" id="33314872"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01335"/>
    <dbReference type="OrthoDB" id="296931at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03376">
        <property type="entry name" value="TPP_PFOR_porB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051479">
        <property type="entry name" value="PorB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008819">
        <property type="entry name" value="PRK11865.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42897">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42897:SF2">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="3">
        <location>
            <begin position="57"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="331" mass="35981" checksum="533B251F7E4AEF9F" modified="2017-09-27" version="1">MAVRKPPITTREYWAPGHAACAGCGCATALKLATKAFSEAMEEKYGDPNAFAIAQATGCMEVVSAVFPYTAWKVPWVHVAFENAAAAASGVEAAWKKLGRKGKILAIGGDGGTADIGMQALSGMLERWHNAVYLMYDNEAYMNTGIQRSSSTPYGAWTTTSPPGKYSIGEDKPKKWVALIAAAHQVPYVATASIGNPFDFVKKMKTAAKVDGPAFVQVHCTCPTGWKSPLEKGVEIARLAIETGVWPLFEIENGDFFNIKIQAPGGGAKVKREGGRVVAIEFKKPIEEYLKLQGRFKHLFKQPEAIDQLREQIKAMWKTLGVEVTLPKPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5L9</accession>
    <name>A0A218P5L9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sugar kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_01380</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853338.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5L9"/>
    <dbReference type="GeneID" id="33314881"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01380"/>
    <dbReference type="OrthoDB" id="26949at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01942">
        <property type="entry name" value="ribokinase_group_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053612">
        <property type="entry name" value="ADP-R1P_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002139">
        <property type="entry name" value="Ribo/fructo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041134">
        <property type="entry name" value="R1P_kin_Thcocales"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10584:SF166">
        <property type="entry name" value="RIBOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10584">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00990">
        <property type="entry name" value="RIBOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="5">
        <location>
            <begin position="6"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="296" mass="32209" checksum="AF05B1C4430D4A8D" modified="2017-09-27" version="1">MQKLDVIGIGNLNYDIIMLLDRFPDFHEKVNAKEAYFGLGGAAANTITWLAHFGLRTGYLGAVGRDEIGEAHMSYFKKIGVDTSGIQIVDAPSGVAVAMVHGDDKRIVKYPGANLLKRLDFDYLSRARHIHMSSNPPETIEGVVKFASERGITISLDIGEAVLPEGVEERVDYLMMNEDEYRRKFGSLDLSLSRAKNLVVTLNGGGAMVRDERGNVAEIRGLSAKVVDSTGAGDSFDAGVIYGVLNGWTLEDSARLGMLLAYLTVQKVGARSAVIPLDEVVRIAEELGMELPFSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5P7</accession>
    <name>A0A218P5P7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphate ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01545</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853368.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5P7"/>
    <dbReference type="GeneID" id="33314914"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01545"/>
    <dbReference type="OrthoDB" id="85148at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016675">
        <property type="entry name" value="UCP016666"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09910">
        <property type="entry name" value="DUF2139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016666">
        <property type="entry name" value="UCP016666"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="487" mass="53989" checksum="A1B8AD5735D93E74" modified="2017-09-27" version="1">MLLPNYRFPGRYGPEWGSGGIFGLRYHNGTLYFTLAFEAEAHFIDVRSCEEKTYDFTLLGEAPTSGGDTYNAVETVDGFIYFGGWVHAPAVYREDRRILFNNKYSHVHVYDTEEGSVKLLWKDSIHHETDWAGEVSDIIYDPYGDRLLLAREDGHANLGVYSLDRRTGRAEALIHEPSPKGTRVHDTAFFGIGNNFTEGLREFRALDLISGRWETFKPGESVDGRPYIRPELGAMASSYNRAFAFVRGGIFAGNPFMGEELSFYRLFDFYTFYAPFRVNAINAGGGILTAFNAHHDAVYRPDPSDPRGWASTNTITGPSVLVYVAPPMVKIVGAFGARVTSIEKMNGKLLVATNSAPNTGAREATPFDTGNRDIVLLDEKVLQERPPAVSFSLPLALPGMAKELGAGTFGGFPLDGYRESRAVFHLSGDNRLTVYEYDLSLPAGEATAETFELKAGKNILDLSSFSGIVSFELEKPEKRGKVRVELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5S4</accession>
    <name>A0A218P5S4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_01710</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Potential transporter for phosphate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853396.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5S4"/>
    <dbReference type="GeneID" id="33314947"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01710"/>
    <dbReference type="OrthoDB" id="101311at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101:SF80">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="327"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="349"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="378"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="403" mass="42765" checksum="E3FB6462F6F0B2BC" modified="2017-09-27" version="1">MDPWLLITIVLGFAMAWAIGANDAANSMSTAVGAKAITPKQAVVIAGLLEFTGAYFFGKSVTETIRKGILNPTMITDPNVLIYGSMAALLAATLWLVIATKFGLPVSTTHSIIGGIVGYGIVYAGTAIVNWGKMGQVVLSWILSPIIGAFMAFLVFKAMTRSIFETKDPVRNARIWSPFWIGLAFVVIGTMFYIKVLHGGDLKTGVMMYGLPAGVVTFIVTYLLIRLRFRSADPLINVESIFKKAQLVTSSYVALAHGSNDVANAIGPVAAVYAVATMGLSGMQVPVPRWILAMGGLGITIGVATYGYKVMETVGKKITELTNTRGFTIQFSAATVVLVSSWLGLPISTTHVVVGAVIGVGLARGVKAINKDIVRDIIISWFVTVPVAALISAAIFKILMLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5S7</accession>
    <name>A0A218P5S7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cation:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_01740</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853401.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5S7"/>
    <dbReference type="GeneID" id="33314953"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01740"/>
    <dbReference type="OrthoDB" id="84883at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007208">
        <property type="entry name" value="MrpF/PhaF-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009242">
        <property type="entry name" value="PRK12599.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34702">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER SUBUNIT F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34702:SF1">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04066">
        <property type="entry name" value="MrpF_PhaF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="87" mass="9403" checksum="8F3A0E88973BA7EB" modified="2017-09-27" version="1">MMEQVFFYATLIVMVAGFISVLRIMLGPSVPDRVVGVDTLNTLVVAAMILLGAAYDRTIYIDIAIVYALLSYIGTLAVARYLQGGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5X8</accession>
    <name>A0A218P5X8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DNA polymerase II large subunit</fullName>
            <shortName evidence="14">Pol II</shortName>
            <ecNumber evidence="14">2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Exodeoxyribonuclease large subunit</fullName>
            <ecNumber evidence="14">3.1.11.1</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">polC</name>
        <name evidence="19" type="ORF">A3L08_02010</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 14">Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 21">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22 21">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2 14">Heterodimer of a large subunit and a small subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 14">Belongs to the archaeal DNA polymerase II family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="14"/>
    <dbReference type="EC" id="3.1.11.1" evidence="14"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853451.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="GeneID" id="33315007"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02010"/>
    <dbReference type="OrthoDB" id="7529at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008310">
        <property type="term" value="F:single-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00324">
        <property type="entry name" value="DNApol_II_L_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004475">
        <property type="entry name" value="PolC_DP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056172">
        <property type="entry name" value="PolC_DP2_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056171">
        <property type="entry name" value="PolC_DP2_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016033">
        <property type="entry name" value="PolC_DP2_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00354">
        <property type="entry name" value="polC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003103">
        <property type="entry name" value="PRK04023.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011303">
        <property type="entry name" value="PRK14715.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42210">
        <property type="entry name" value="DNA POLYMERASE II LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42210:SF1">
        <property type="entry name" value="DNA POLYMERASE II LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24846">
        <property type="entry name" value="PolC_DP2_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24844">
        <property type="entry name" value="PolC_DP2_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03833">
        <property type="entry name" value="PolC_DP2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016275">
        <property type="entry name" value="PolC_DP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 14" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="8 14" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 14" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6 14" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA polymerase II large subunit DP2 N-terminal" evidence="16">
        <location>
            <begin position="12"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase II large subunit DP2 central" evidence="17">
        <location>
            <begin position="326"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase II large subunit DP2 catalytic" evidence="18">
        <location>
            <begin position="790"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1223"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="1240"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1252"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1286"/>
            <end position="1299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF24844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF24846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ASJ06188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="1316" mass="149864" checksum="DDE47FFEEFF61D21" modified="2017-09-27" version="1">MSEELYSPEMKAYFESLQREIDRAYEIARKARAQGKDPSLEVEVPQATDMAGRVESLVGPKGVAPRIRELVKEYGKEIASLKIVDEIIDGKFGDLGSKEKYAEQAVRTALAILTEGIVSAPLEGIADVKIKRNTWADNSEYLALYYAGPIRSSGGTAQALSVLVGDYVRKKLGLDRFKPSEEHIERMVEEVDLYHRAVTRLQYHPEADEVRLAMKNIPIEITGEETDKVEVSHRNVPGVETNHIRGGAILVMAEGVLQKAKKLIKYIDKMGIDGWDWIKEFVEAKEKGNSTEEKPSDGSKAEEAGRDEVREEVKKGFYYELYERFKANIAPNKKYTKEIIGGRPLFAEPSTNGGFRLRYGRSRVSGFATWSVNPATMLILDEFIAIGTQMKTERPGKGCIVTPATTVEGPIVKLKNGAVVKVDDYETALKVRNEIDEILYVGDALVNFGDFVENNQTLLPANYVEEWWVQELVKAIADTYEVELKPFEENPWEAVEEAAEYIEVDPEFLGELLHDPLRVRPDVETAIHLSKVLDIPFHPYYTLYWNTLRPEEVEELQKALLNAQIEWDEFRKNKFARKVILDNDPRIKRYLELLGLPHRLERTEDRKKVIVIDYPWAAALLTPLGNLEWEFRAKPFFTVIDIINENNRIKLRDRGISWIGARMGRPEKAKERKMKPPVQVLFPIGLAGGSSRDIKKAAEEGKVASVEIAFFKCPNCGHIGPEHLCPRCGTRKELLWHCPKCNVDYTQSEAENFDFRCPKCGAELSPYARRTIKPSELLRQAMDNVKVYGIDKLKGVQGMTSGYKMAEPLEKGLLRVKNDVYVFKDGTIRFDATDAPITHFKPKEIGTSVEKLRELGYTHDFEGKPLERDDQILELKVQDVILPYEAGRYLLKVARFIDDLLEKFYGLPRFYNAEKMEDLVGHLVIGLAPHTSAGIIGRIIGFSDVLVGYAHPYYHAAKRRNCDGDEDAVMLLLDALLNFSKYYLPEKRGGKMDAPLVVTTRLDPREVDSEVHNMDVVRYYPLEFYQATYEMKSPKSIKFIERVEDRLGKPEMYEGIKFTHDTDDIGLGPKMSLYKQLGDMEEKVERQLTLAERIRAVDEHHVAETIINSHLVPDLRGNLRSFTRQEFRCVKCNTKYRRPPLTGKCPKCGGKIVLTVSKGAIEKYLPTAKMLVTRYNVLDYTRQRICITEKDIKTLFENVFPERQRTLMGFSADVCEKMVKERTGKSNGKNGYLDELKANGKGKRKGGKKEKKTLEPKEKEAKKGPKRSEKKIKPATGLEKAALKRYLVEEKGKEKEQNVKPRKKKKGISLDDFFGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Z6</accession>
    <name>A0A218P5Z6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Proteasome assembly chaperone family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853459.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5Z6"/>
    <dbReference type="GeneID" id="33315015"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02050"/>
    <dbReference type="OrthoDB" id="35908at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004425">
        <property type="entry name" value="MJ0106-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00161">
        <property type="entry name" value="proteasome assembly chaperone family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610:SF8">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159659">
        <property type="entry name" value="Cgl1923-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="209"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="242" mass="27125" checksum="DC82C4A4CE3EF082" modified="2017-09-27" version="1">MENDKPVEIVLPEVKNPILIEGYPGVGLVGHIAANFLAKELKMDMIGYVESPFIPPMAMILEGKPNPPLRFYGKDNLIVAIADIYIPPTLVNEIARELVKYLKEMDAGKVVSMGGIGIGFFKEQMDVWGVGAREELNKELEENGVKILQYGSIMGMSGRLLWEASRNNLDAYVLLGETFGDRPDPRAAANVIEALKKLVPLEVSTEPLLQEARAIEEQLRRMHEQMEQARKKAQQQYESIYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P605</accession>
    <name>A0A218P605_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_02110</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853469.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P605"/>
    <dbReference type="GeneID" id="33315027"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02110"/>
    <dbReference type="OrthoDB" id="371687at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051081">
        <property type="entry name" value="HTH_MetalResp_TranReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33154:SF38">
        <property type="entry name" value="HTH ARSR-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33154">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ARSR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="191" mass="22582" checksum="B798C79BBF6C14B8" modified="2017-09-27" version="1">MAKVKVITDPEVIKLMLEDTRRRILSLLRNKEMTISQLSEILGKTPQTIYHHIEKLKEAGLVEVKRTEMKGNLVEKYYGRTADAFYINLYLGDEELRYFARSRLKTKLEIFKALGYEFNDEELLNTMDELLKKEHEYKTEISKEIEANEERLKDFPDEDIIHAIEWLSMAKMGRDEETLALLRRLGEILKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P607</accession>
    <name>A0A218P607_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NAD kinase</fullName>
            <ecNumber evidence="8">2.7.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">ATP-dependent NAD kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ppnK</name>
        <name evidence="8" type="synonym">nadK</name>
        <name evidence="9" type="ORF">A3L08_01965</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>NAD(+) + ATP = ADP + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18629"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the NAD kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853444.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P607"/>
    <dbReference type="GeneID" id="33314998"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01965"/>
    <dbReference type="OrthoDB" id="77798at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002984">
        <property type="entry name" value="PRK03708.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF43">
        <property type="entry name" value="BIFUNCTIONAL NADP PHOSPHATASE_NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 8" id="KW-0520">NAD</keyword>
    <keyword evidence="6 8" id="KW-0521">NADP</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="67"/>
            <end position="68"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="137"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="178"/>
            <end position="183"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="278" mass="31234" checksum="38BE30695717ADC1" modified="2017-09-27" version="1">MRFGVVARRDKEAALKLAYRVYDFLKVSGYEVIVDEETHQHFPHFREGDVLPLEDFDVDFIIVIGGDGTILRVEHRTKKEIPILGINMGTLGFLTEVEPHEAFFAISKLIEGEYYIDERIKLRTYLKGENRVPDALNEVAVLTGVPGKIIHLKYYVDGGLADEIRSDGLIVSTPTGSTGYAMSAGGPFVDPRLEAVVIAPLAPIALSSRPIIVPAFSTIDIRNLSLTREIILAVDGQFYTYLSPDTEITVKLSPRKARFVRFTNDVYPKYTLRLKNRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P613</accession>
    <name>A0A218P613_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2118 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_02200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853486.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P613"/>
    <dbReference type="GeneID" id="33315045"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02200"/>
    <dbReference type="OrthoDB" id="84532at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019217">
        <property type="entry name" value="DUF2118"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09891">
        <property type="entry name" value="DUF2118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019115">
        <property type="entry name" value="UCP019115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="164" mass="18878" checksum="6C8D7858A23EB6D4" modified="2017-09-27" version="1">MERMPRLYVEVDAGECVVQGKATRDCVIIQGNIEVWLEEGDELPEFIDLEDAKFLAKEVYDRFYLYVDRMEGRMLADVVLVLPDGRTRIYLKKGDELMILPVEGYTKTIIANVGNRVRKGDAFAAVTTRKGEVHYLKPPKTGTVVFIDEITDRPHYVYYILPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P616</accession>
    <name>A0A218P616_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Peptide transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_02135</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853474.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P616"/>
    <dbReference type="GeneID" id="33315032"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02135"/>
    <dbReference type="OrthoDB" id="169602at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045035">
        <property type="entry name" value="YSL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645:SF0">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00173">
        <property type="entry name" value="EDTRNSPORT"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="205"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="272"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="343"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="395"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="435"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="506"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="547"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="576" mass="60454" checksum="920BCBB60336F835" modified="2017-09-27" version="1">MADANWKRGDSDENYREITPAAVILGVIWGAFMAASFTYAGMIMGFTSGGSAIAAIVGWGVLRGILKKGTIVENNIVQTIASAVNISVSGVIFTIPALYIMGLHQEINTAYFFLATAAGAILGITFIIPLRKQMIEIDRLRFPTGTAVATVLKTPGSGIEKARLLFLGMGISALVYLIQQFPVLGLPEIIPEYIDLGAILHLPDWVSLAMALSLMVFGMGLITGRNGLIVLAGGVLSYYIITPIVKSLGWLPSDVTGSAVSDFVYANMTRPLGIGMLLGGSIAGLILSMPVIIVALRSIANASRLGTGRNEELPIKYLYAGITLAFLLLLVTTYKLGNLGIGRSLLTAIVGVAWIFVASLLVAMSTGMTDWSPVSGLSLVSVMILLYLTGKQVPLTILLGATVGVAISGAADMMQDLKTGHLVGGIPSRQQKVELLTAWIGPIIALTVVDLIWKAYGIGNETVPAPQAMALKSMVDSILGGNVPVDKFIAGGILGFALSLSGIPGLGVLVGLSMYLPMLYILPYGLGCIAHEVVKRKKGSEFITEKVLPVAAGLMVGEAAMTLLFAVLTVAGVIHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P625</accession>
    <name>A0A218P625_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853488.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P625"/>
    <dbReference type="GeneID" id="33315047"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02210"/>
    <dbReference type="OrthoDB" id="100157at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="143" mass="16156" checksum="CA2D51F141985E51" modified="2017-09-27" version="1">MRLHKAVIVASIVLLLVATMAGSYLIRWLSVLFLGAFLAFFLFGIEIQVARPRLKRGMKVERKTDVERTVSLIQKARSGRVARSLIEEKIVDIYATLSDDYNSAYRSLLSEPNEAIKALRSEGDFLDNLERALKIVEADLNED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P635</accession>
    <name>A0A218P635_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_02365</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853515.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P635"/>
    <dbReference type="GeneID" id="33315078"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02365"/>
    <dbReference type="OrthoDB" id="85776at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022121">
        <property type="entry name" value="Peptidase_M73_camelysin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12389">
        <property type="entry name" value="Peptidase_M73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="198" mass="21756" checksum="85970BFF464A26DB" modified="2017-09-27" version="1">MKRAMVVGVLGLLVFLAGVKFGVSYFSDVAKSEGNQISTGEFDIGISRDGERFYDDYKLFSFDDLQPGEERTVDFYIKNRGDYPISFVGMVFNITDLEDGSPSKAEALVDKTPDTGELSKYLIIKDFRVSQDGDEKVLSDYIGKSLKDLNMSELQIFSGSLPENGILKVTLTVQLSPAAGNDCLTDTSKICIRITAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P668</accession>
    <name>A0A218P668_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Sodium:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">A3L08_02430</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853526.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P668"/>
    <dbReference type="GeneID" id="33315091"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02430"/>
    <dbReference type="OrthoDB" id="12029at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562">
        <property type="entry name" value="NAPA-TYPE SODIUM/HYDROGEN ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562:SF3">
        <property type="entry name" value="SODIUM ION_PROTON EXCHANGER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="55"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="264"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="286"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="355"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="11">
        <location>
            <begin position="13"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="380" mass="40369" checksum="47F72ADC65C33425" modified="2017-09-27" version="1">MDVFLELALILIVAKLFGYLTVRLGFPAALGQLIGGILIGPSVLGLVGYDEGVKLLAELGVVMLLFLAGLETDVEEFKHVGVPAFIIAALGVFVPFIFGYLGAMAWGYSNVQAMFLGGILTATSVGLTTSILMEMKKLRTRVGTTILAAAVVDDVLGIIVLTILVGINTRGSVYAKDLMIIFGEVAVYFALGLLVGHPAVKEALKASEKITLPETLTAVAIAIMLIFAYLAEQFQIAGITGAYLAGILVASTEEAREINNKTMTIGYSLFIPIFLVSIGIESDVRVLAHAGVFALVYSLLGILGKIFGCGLGAFVSRFKPREALQVGVGMIPRMEVALIMANVALREGAFDRGTFAIPVTMVVITTIVTPFLLKWAFSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P686</accession>
    <name>A0A218P686_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">A-type ATP synthase subunit C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">atpC</name>
        <name evidence="9" type="ORF">A3L08_02590</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the V-ATPase V0D/AC39 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853557.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P686"/>
    <dbReference type="GeneID" id="33315124"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02590"/>
    <dbReference type="OrthoDB" id="4272at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.50">
        <property type="entry name" value="ATP synthase (C/AC39) subunit, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1690.10">
        <property type="entry name" value="V-type ATP synthase subunit C domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00314">
        <property type="entry name" value="ATP_synth_C_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036079">
        <property type="entry name" value="ATPase_csu/dsu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014272">
        <property type="entry name" value="ATPase_V0-cplx_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002843">
        <property type="entry name" value="ATPase_V0-cplx_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050873">
        <property type="entry name" value="V-ATPase_V0D/AC39_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035067">
        <property type="entry name" value="V-type_ATPase_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044911">
        <property type="entry name" value="V-type_ATPase_csu/dsu_dom_3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02923">
        <property type="entry name" value="AhaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002269">
        <property type="entry name" value="PRK01198.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38682">
        <property type="entry name" value="V-TYPE ATP SYNTHASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38682:SF1">
        <property type="entry name" value="V-TYPE ATP SYNTHASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01992">
        <property type="entry name" value="vATP-synt_AC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103486">
        <property type="entry name" value="V-type ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 8" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="5 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="366" mass="41792" checksum="74087026CC1A3850" modified="2017-09-27" version="1">MEPEAVSGILNTTLAVVFTWVGYKTAKMIWKYTPYSYPNARIKAMEAKLLTEQRFNELAESKTLQNFVVSLEDTDYRSYFANLSSYDVESIERALEKALAGTYELMAKILPKRVSPFFRLLLEEWDVRNITSVVKAKLAGEPATDYVVEIGTMLPKVKAMAETKTMEEMLVILEGTPYEEPYQRLYLNEIDVSAFETELYRMHYGKLLEYALSRKEDERVILEEFVRLRIDKLNILTVLRAKAAGMSAEEIEPMLIPGGSVRLDPLLHIDDLSMALAELDSTKYGQVIRGVREEVERDLGVLEKALDGYILERMNELNRFYPLSVAVPLGYILQKEREVRKLRAIAKLISDGIPPERIKELAGDVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6C2</accession>
    <name>A0A218P6C2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sugar transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_02815</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853601.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6C2"/>
    <dbReference type="GeneID" id="33315169"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02815"/>
    <dbReference type="OrthoDB" id="18784at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051393">
        <property type="entry name" value="ABC_transporter_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30193">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30193:SF37">
        <property type="entry name" value="INNER MEMBRANE ABC TRANSPORTER PERMEASE PROTEIN YCJO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160964">
        <property type="entry name" value="MalF N-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="76"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="301" mass="33899" checksum="3D50C2C3A2F46996" modified="2017-09-27" version="1">MRKTTTIALFLILPGIAAFLFFNLWPIIYSVYLAFTNAQLGNFPIYNPESAAGPLRFVGLENFRWILSNDGFREAFKWTWIFVATSVTLKVLAGIFLGLLYNSKYVKGKAVYRSLLIIPWALPLLFSVTVWKFMFDPIFGPINQILKSLGVSTLPNWINDPTWAFLALNIIEVWLAYPFMMTVITAALQSVPDTLVEAAIIDGANYWQRVRHVVLPIVGKPVAFATILTSAASFQYFMVPYIYNAGLFENKFLLLYGFRKAFGASPHYGRASAVMIIATLILAVYMYVNVRITKLQEGAKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6F1</accession>
    <name>A0A218P6F1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6F1"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02930"/>
    <dbReference type="OrthoDB" id="100690at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="105" mass="12352" checksum="FF1B1FB1948A1B02" modified="2017-09-27" version="1">MRRWTFVLPFIYFLFLFALTFMDFPRQVNRPWMWIQIGGLVFMIVLGSYLDRNIPTAELTVIGAFTLLWLALAFLIPLKEEYFALLGLIIELGIVIIVEWFFRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6F4</accession>
    <name>A0A218P6F4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Maltodextrin-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_02820</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853602.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6F4"/>
    <dbReference type="GeneID" id="33315170"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02820"/>
    <dbReference type="OrthoDB" id="42146at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055052">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015144">
        <property type="term" value="F:carbohydrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901982">
        <property type="term" value="F:maltose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042956">
        <property type="term" value="P:maltodextrin transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015768">
        <property type="term" value="P:maltose transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13657">
        <property type="entry name" value="PBP2_Maltodextrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006060">
        <property type="entry name" value="Maltose/Cyclodextrin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006061">
        <property type="entry name" value="SBP_1_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30061">
        <property type="entry name" value="MALTOSE-BINDING PERIPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30061:SF50">
        <property type="entry name" value="MALTOSE_MALTODEXTRIN-BINDING PERIPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00181">
        <property type="entry name" value="MALTOSEBP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01037">
        <property type="entry name" value="SBP_BACTERIAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="26"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="29"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="438" mass="48282" checksum="25BAD69D2588C8E6" modified="2017-09-27" version="1">MKKGLFALLLVGVLVLSVVASGCIGGGETTSSPSSTSESTTSTTTSSSATETTAPETDCGSGDIVIWHAMQPNELEVFQSLAEEYMAMCPGVTITFEQKPDLESALKAAIPTGQGPDLFIWAHDWIGKFADAGMLEPIDDYVTDDILKDFAPMAVDAIQYKEHYYAMPFAAETVAVIYNKNMVSEPPRTFDEMKAIMEKYYDPDNEKYGIAWPINAYFISGIAQAFGGYYFDDKTEQPGLDKPETIEGFQFFFQNIWPYMAPTGDYNTQQSIFLEGRAPMMVNGPWSINDVKKAGIDFDVVPLPPITKDGKEYWPRPYGGVKNIYFAAGIKNKEAAWKFVKWFTTSPDVIKELSLQLGYIPVLKPVLDDPEIQADPVIYGFGQAVQHAYLMPKSPKMSAVWGGVDGAINEILQDPANADIPAILQNYQQQILDNMNGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6I1</accession>
    <name>A0A218P6I1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF58 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02995</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853632.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6I1"/>
    <dbReference type="GeneID" id="33315205"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02995"/>
    <dbReference type="OrthoDB" id="3263at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002881">
        <property type="entry name" value="DUF58"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34351:SF1">
        <property type="entry name" value="SLR1927 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34351">
        <property type="entry name" value="SLR1927 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01882">
        <property type="entry name" value="DUF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF58" evidence="1">
        <location>
            <begin position="185"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="427" mass="47577" checksum="9FB4E4943A038373" modified="2017-09-27" version="1">MKRAEVVLSLAAVVAGTAYLLGSPAAALIGVALMAHYSLARMAFKPKVRVERSLPERGTEREPVKARVRIENLSDVAGLVRVRETSEKAFARGLKAFVRPGERKYLEQTVVPQAKGRINLKAEAVFEDELGLFRASFPVIGRGEMAVFPSQRSIREAMREKRQVEALAEAERALGIGAETLEFQELREFLPGDDITKIDWKATSRLQKLIIRVFKRETMADIYLLINVDPRFRRELKAGKTDYLVLIIAQLTAYFRRFGHGIKVLAYDEHGIVRAIENPLDPLSVVHELGLRAERGLPALRPAKVSRGSSLGTLVARLKSGSEAHGVLKAALKVPAGSYVIVVDDLGLHPGEIMKASRLLERRGSKVVLLYPNPVFFVDKSTLSEDEVEVLYNAYRERKELMKKVMGWVKVIEVGPKDLLPVVVSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6J9</accession>
    <name>A0A218P6J9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">N5-carboxyaminoimidazole ribonucleotide mutase</fullName>
            <shortName evidence="3 4">N5-CAIR mutase</shortName>
            <ecNumber evidence="3 4">5.4.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">5-(carboxyamino)imidazole ribonucleotide mutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">purE</name>
        <name evidence="7" type="ORF">A3L08_03250</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + H(+) = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate</text>
            <dbReference type="Rhea" id="RHEA:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="EC" id="5.4.99.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AIR carboxylase family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.18" evidence="3 4"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853674.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6J9"/>
    <dbReference type="GeneID" id="33315256"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03250"/>
    <dbReference type="OrthoDB" id="9473at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00943"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034023">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01929">
        <property type="entry name" value="PurE_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033747">
        <property type="entry name" value="PurE_ClassI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024694">
        <property type="entry name" value="PurE_prokaryotes"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01162">
        <property type="entry name" value="purE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23046:SF2">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23046">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001338">
        <property type="entry name" value="AIR_carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52255">
        <property type="entry name" value="N5-CAIR mutase (phosphoribosylaminoimidazole carboxylase, PurE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1 3 4" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurE" evidence="6">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR001338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001338-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="143" mass="15173" checksum="1F9DBF403CC6E59B" modified="2017-09-27" version="1">MKVLVVMGSKSDSHIAEKVTSVFDEFGVEYDVEVASAHRNPKKVEELAKKDYDVFIAIAGLSAALPGVIAAHTTKPVIGVPVSAKLGGLDALLSVAQLPPGVPVATVGIDNGKNAALLAVEILALKDEGLRRKLEEYREKMRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6M1</accession>
    <name>A0A218P6M1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GMP synthase [glutamine-hydrolyzing] subunit A</fullName>
            <ecNumber evidence="8">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glutamine amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">guaAA</name>
        <name evidence="10" type="ORF">A3L08_03290</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>XMP + L-glutamine + ATP + H2O = GMP + L-glutamate + AMP + diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B).</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853681.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6M1"/>
    <dbReference type="GeneID" id="33315264"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03290"/>
    <dbReference type="OrthoDB" id="10772at2157"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003921">
        <property type="term" value="F:GMP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01742">
        <property type="entry name" value="GATase1_GMP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000047">
        <property type="entry name" value="GMP synthase [glutamine-hydrolyzing] subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01510">
        <property type="entry name" value="GMP_synthase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004739">
        <property type="entry name" value="GMP_synth_GATase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023686">
        <property type="entry name" value="GMP_synthase_A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00888">
        <property type="entry name" value="guaA_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001975">
        <property type="entry name" value="PRK00758.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922:SF2">
        <property type="entry name" value="GMP SYNTHASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922">
        <property type="entry name" value="GMP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4 8" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="2 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="9">
        <location>
            <begin position="4"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="189" mass="21408" checksum="65CF5DDE974FEA7F" modified="2017-09-27" version="1">MIIIMDNGGQYVHRIWRTLRYLGVEAKIIPNTTPLEEIKAMRPKGIIFSGGPDINKISNCEAILEHYDEFNVPILGICLGHQLIAKHFGGKVGRGDKAEYSLVEIEILEENDIFKGLPRKLKVWESHMDEVKELPPGFKLLARSETCPVEAMKHESLPIYGVQFHPEVAHTERGVDIYRNFAELCGELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6M7</accession>
    <name>A0A218P6M7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Class III signal peptide-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6M7"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03320"/>
    <dbReference type="OrthoDB" id="100210at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="160" mass="17410" checksum="7320B1A7548279CF" modified="2017-09-27" version="1">MLSSATRRKGQVAIEVLFMTAIILTGIVILVPSYLDENRSSTLITYVKSSASSACSYLNSGVTADSQPYSILNPVIEKSNYTSRNFMVSSISSSESGDTITINIQVRYSGKVDLDNDNIAKAIQDFLVRDLLDHTNARMEGKTIYYGGKKVLINVNVVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6N0</accession>
    <name>A0A218P6N0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03420</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853705.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6N0"/>
    <dbReference type="GeneID" id="33315290"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03420"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="73" mass="8719" checksum="15C53E1F74D95A45" modified="2017-09-27" version="1">MNGTVNLEREIRILLQRRGPLSVAFITRFLNEMGIDCTRQKVERVLRNLVLNGVVEAFYNGGRYNRRRHYRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Q0</accession>
    <name>A0A218P6Q0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853688.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Q0"/>
    <dbReference type="GeneID" id="33315271"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03325"/>
    <dbReference type="OrthoDB" id="86130at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="147" mass="16336" checksum="4B9ECE9C73B83886" modified="2017-09-27" version="1">MKRGQISLDFLFAITLIAITVLNMVYIANMQRSHAETFDATTRLKIFSVDIRDTVTKVYASGNGFSAKKESPIPLTGGDSITVRLSSKDNMIIITGNIGGRQYTTVQQSPVPIYQDESIQITPDKSEIWIKARERGGKVYVELSYSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Q2</accession>
    <name>A0A218P6Q2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal-dependent phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853726.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Q2"/>
    <dbReference type="GeneID" id="33315317"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03560"/>
    <dbReference type="OrthoDB" id="103508at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039967">
        <property type="entry name" value="MJ1020-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40517">
        <property type="entry name" value="METAL-DEPENDENT PHOSPHOHYDROLASE, HD SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40517:SF1">
        <property type="entry name" value="METAL-DEPENDENT PHOSPHOHYDROLASE, HD SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD/PDEase" evidence="1">
        <location>
            <begin position="48"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="258" mass="29421" checksum="0FC50114881F0CB6" modified="2017-09-27" version="1">MYTEEELLKEIRELMGDDELFSMYERTFREYHYYFDTTNYIVLNVYGFNDHGPVHVLLTTRRALELLSIIKKFGIQTTAEKLGKPLRWSKFIVAFGALFHDIGNMIHRINHYGFSAFLAEPIIEKLVGEFEDGDPLLLKALTLNAIYTHDEAVPCTTIEGSLVTIADGCDMEEGRSRLVHKKDKVDIHAVSALAIERVKITEGDEEQPILIEIWMKHPAGIFQVDEILTKKVKSSLLSGKVRLRIHTGTDGEVVEKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Q7</accession>
    <name>A0A218P6Q7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF5305 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03390</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853699.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Q7"/>
    <dbReference type="GeneID" id="33315284"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03390"/>
    <dbReference type="OrthoDB" id="86028at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035185">
        <property type="entry name" value="DUF5305"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17231">
        <property type="entry name" value="DUF5305"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="334" mass="37586" checksum="77D2CE583FA6EC02" modified="2017-09-27" version="1">MKELSLDKAKAIKITLAVFIGMSVLFGAYSAMAYSTDPTTTQVTYKTLYMEKGELSHAGFFSNETIYKNGTSLKYYPEKITGLIVGNYDYSIAPETEGNYRAVLRTDYYVVSNKERIYLMNTTQEAWSGKFSGSFSIPVTFNISKINGELKVLREGTGLFRASVDTYLLVEVQMAGKEPFTHRITLAKDTSGMLKLTEPEKDYKKVERYTNTTANSINFAGKEVLVSTGRTVFPALALLFMMPPLGFAYTHREKKPKDELKGLRKFMVEGVPSEVEAMDPVELSSAEDLERVFDLVDKPIVHYTKDGHDVYAVVDGELVYEYRKPLPREGKKAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6V1</accession>
    <name>A0A218P6V1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">AI-2E family transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_03800</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853770.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6V1"/>
    <dbReference type="GeneID" id="33315365"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03800"/>
    <dbReference type="OrthoDB" id="137390at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716:SF71">
        <property type="entry name" value="TRANSPORT PROTEIN MJ1177-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="5"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="288"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="334"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="348" mass="38836" checksum="DC5FB33CA00FB5A4" modified="2017-09-27" version="1">MRTETIAWSIAVIIILYIAWKTVSPLVTPIFFGVVLAYASYPIHRRLAVRTGKKESAALLTLVTVGLGGMVTFELIKISVQVAVSFYNSTVDVFSWIMAQPLPQDVLEFIQRFFNQLIPRLSEYISRGAFSLPMYLLQLLVFLLTFYYTLAYSGEIYRQIVLSLPERNRHIGEEILESLNKTLGALVRAWLVLNVIKGALMTIGFIIFGVSDLYTAIVAGFLTFLFSFIPLFEGWMIWLAATAYFAVNGAYLRAVGIALYGLFLVSPMPDYTVRPMMVARDTNLDETLVFIGMIGGTWAMGVKGLIIGPIVLNLLLVLLKEWKRIVSAKEPSHQPSPSPSAPSPHPQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6W5</accession>
    <name>A0A218P6W5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF2391 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6W5"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03745"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024464">
        <property type="entry name" value="DUF2391"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09622">
        <property type="entry name" value="DUF2391"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="169" mass="19197" checksum="7BF7BB2801420F1E" modified="2017-09-27" version="1">MMNPEPERTKDERRTTMEEKLEEIYEGIEELRKEFDGRKAPDALGWDDIAQEVVGAITFALPFLFTGELWEIAKDISLERSLAIFLMTLVVAYLFIAKSRIGNLKREEMFHIPKRLFTVAVIAYLTSAGLIYLYGINRIADFNVAQYLNATVLVSTFAVIGAITVDLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6X7</accession>
    <name>A0A218P6X7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glutamate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_04000</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853806.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6X7"/>
    <dbReference type="GeneID" id="33315404"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04000"/>
    <dbReference type="OrthoDB" id="27922at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00368">
        <property type="entry name" value="Molybdopterin-Binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.20:FF:000035">
        <property type="entry name" value="Iron hydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028261">
        <property type="entry name" value="DPD_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000283">
        <property type="entry name" value="NADH_UbQ_OxRdtase_75kDa_su_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054351">
        <property type="entry name" value="NADH_UbQ_OxRdtase_ferredoxin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009410">
        <property type="entry name" value="PRK12771.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42783">
        <property type="entry name" value="GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42783:SF3">
        <property type="entry name" value="GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13510">
        <property type="entry name" value="Fer2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14691">
        <property type="entry name" value="Fer4_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22117">
        <property type="entry name" value="Fer4_Nqo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53706">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase, domains 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00641">
        <property type="entry name" value="COMPLEX1_75K_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="953" mass="105843" checksum="096C607B8149B37C" modified="2017-09-27" version="1">MVKITLNGKEIDVPAEKPLIEFLREIGEHVPGFCYTEELEPYGSCRLCLVSTPRGVTTSCTLKPMEGLSVETLSDEVVSMRKTALELILSDHYGDCIGPCQNGCPAHSDVQGYLALIAMGKYHEAVKLMKEKYILPAVLGRVCPAFCEEACRRNLVEEPLAIRQLKRFAADYDLEHGPWMPEIPPSTGKRVAVVGGGPAGLACAYYLRTMGHEVTIIEAMPELGGMMRYGIPPYRLPRDVLDKDIATVINTGIEVKTNTALGRDVTLEELRKEYDAVFLGVGAWRSRKMGIPGEELEGVMHGIEFLRKVNTGEKVELGERVIVVGGGNTAMDVARTALRLGAKVTVVYRRSKAEMPANEREVEEAMEEGVEFLFLTNPVRILGDGKVEEVELVKMRLGEPDASGRRRPIPIEGSEFRVKADNVILAIGQYCDEEFLKSLGIEAKRGKAVVDEVTLQTNLEGVFAGGDLVLGPSTVIESIATGRRAAVMIDMYLKGKLEKAKEVLTEPEKHIEEVLADDDLYRVLFDLRPYNHWKKVTEKDYEHVERKPRVKVKLLDPERRERTFEEVEPALTEEQVLEEAKRCMSCGCMEVFRCKLREYATLYNARQDAFEGEGNRFEIDESHPWITLDNNKCVLCGQCVRFTHDVAGEGVLDYLFRGFKTRISPPLGEGLGDMEGLFLGEMIDLCPVGAITERLPFVKPGPWKTEPVKTVCNGCSFACEMNVETYDGMLVRASRAEESWNKHLCDLCRFARPWAEDLAGPLLNGKPVSWEEARRFIEEKDYTLILTPELTNEEIAFFKAFAEKKGIPIGSTVEGAISTATLDDVRKAKRVLLKADVEKYPLLKILLKGKEIVEEDYDVAILEGGEPLDVPTLILHPGVNAAGLIKAGITGIPEVEAYVVMGRTEKELPGDVLVLPAGLWAAKEGTVTNAFGMELRVGKAVEEVNRALELFSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P707</accession>
    <name>A0A218P707_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rpl23</name>
        <name evidence="9" type="ORF">A3L08_04055</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds to 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">Part of the 50S ribosomal subunit. Contacts protein L29.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853817.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P707"/>
    <dbReference type="GeneID" id="33315415"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04055"/>
    <dbReference type="OrthoDB" id="7751at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001084">
        <property type="entry name" value="50S ribosomal protein L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_A">
        <property type="entry name" value="Ribosomal_uL23_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_B">
        <property type="entry name" value="Ribosomal_uL23_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019985">
        <property type="entry name" value="Ribosomal_uL23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_uL23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001014">
        <property type="entry name" value="Ribosomal_uL23_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011118">
        <property type="entry name" value="PRK14548.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03636">
        <property type="entry name" value="uL23_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11620">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L23A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00050">
        <property type="entry name" value="RIBOSOMAL_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00063905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="86" mass="9847" checksum="98B83863349ACF84" modified="2017-09-27" version="1">MDPYKVIVKPVVTEKAVAMIENENKLTFIVDKRATKQDIKRAVEEMFEVKVEKVNTLITMRGEKKAYVKLKPEYSASEVAARIGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P727</accession>
    <name>A0A218P727_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytidylate kinase</fullName>
            <shortName evidence="10">CK</shortName>
            <ecNumber evidence="10">2.7.4.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cytidine monophosphate kinase</fullName>
            <shortName evidence="10">CMP kinase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cmk</name>
        <name evidence="11" type="ORF">A3L08_04185</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>CMP + ATP = CDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:11600"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>dCMP + ATP = dCDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:25094"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57566"/>
            <dbReference type="ChEBI" id="CHEBI:58593"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the cytidylate kinase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.25" evidence="10"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853841.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P727"/>
    <dbReference type="GeneID" id="33315441"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04185"/>
    <dbReference type="OrthoDB" id="31096at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036430">
        <property type="term" value="F:CMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036431">
        <property type="term" value="F:dCMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006220">
        <property type="term" value="P:pyrimidine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02020">
        <property type="entry name" value="CMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00239">
        <property type="entry name" value="Cytidyl_kinase_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011892">
        <property type="entry name" value="Cyt_kin_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011994">
        <property type="entry name" value="Cytidylate_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02173">
        <property type="entry name" value="cyt_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13189">
        <property type="entry name" value="Cytidylate_kin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="12"/>
            <end position="20"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="197" mass="22188" checksum="AA68240DA42CE2E9" modified="2017-09-27" version="1">MPKGCLVITVSGLAGSGTTTLCRNLARHYGFKHIYAGLIFRQMAKERGMTLQEFQEYAELHPEIDREVDRRQVEAAKECNVVIEGRLAGWMVKEADLRIWLDAPIMERAKRVARREGISVEEAFVQIAEREKSNRKRYLNLYGIDIDDKSIYDLIINTAHWNPDGVFAIVKAAIDHLYPDGDAGSGSNPGNKKAEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P741</accession>
    <name>A0A218P741_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PadR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_04290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853855.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P741"/>
    <dbReference type="GeneID" id="33315462"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04290"/>
    <dbReference type="OrthoDB" id="56053at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052509">
        <property type="entry name" value="Metal_resp_DNA-bind_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33169">
        <property type="entry name" value="PADR-FAMILY TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33169:SF14">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR RV3488"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator PadR N-terminal" evidence="1">
        <location>
            <begin position="21"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="109" mass="12989" checksum="6CFB4B4A1C4F171C" modified="2017-09-27" version="1">MTTPMERLRNKITKEVLWLYILRLLKERPMYAYELKERIKEAFNFEPATVSSYVVLYKLEKEGYVTAEWQESQTGKPSRKYYRLTPEGEKLLEDGIAFLEDMLSKLKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P742</accession>
    <name>A0A218P742_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein uL13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl13</name>
        <name evidence="7" type="ORF">A3L08_04245</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 5">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853849.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P742"/>
    <dbReference type="GeneID" id="33315453"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04245"/>
    <dbReference type="OrthoDB" id="7668at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1180.10:FF:000012">
        <property type="entry name" value="50S ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_uL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_uL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005823">
        <property type="entry name" value="Ribosomal_uL13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023563">
        <property type="entry name" value="Ribosomal_uL13_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005755">
        <property type="entry name" value="Ribosomal_uL13_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_uL13_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01077">
        <property type="entry name" value="L13_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005004">
        <property type="entry name" value="PRK06394.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002181">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00783">
        <property type="entry name" value="RIBOSOMAL_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="142" mass="16276" checksum="333AE44E8DD1C7E8" modified="2017-09-27" version="1">MRIINAEGLILGRLASKVAKMLLEGEEVVIVNAEKAIITGNREDIFAKYKQRTELRTRTNPRRGPFYPKRSDEIVRRTVRGMLPWKTDRGRKAFKRLKVYAGVPKEFEGRELETIIEAHMSRLATPKYVTVGEVAKFLGGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P768</accession>
    <name>A0A218P768_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_04460</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853882.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P768"/>
    <dbReference type="GeneID" id="33315496"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04460"/>
    <dbReference type="OrthoDB" id="10874at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002739">
        <property type="entry name" value="PAB1135-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011142">
        <property type="entry name" value="PRK14555.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38816:SF1">
        <property type="entry name" value="EXOSOME SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38816">
        <property type="entry name" value="EXOSOME SUBUNIT, DUF54 FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01877">
        <property type="entry name" value="RNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="151" mass="17695" checksum="8B7F9F5D08FF966A" modified="2017-09-27" version="1">MAKLRAHHIRLTTFIQATEDEDKVLDAIATFIPEEIDEDDVIFDIDETTGFFGNPIKVVNVEIKRSKAVRQFLDYFKELLSEDDRNYLLKHLDEKIDEEGTFYVRFNKQKAYLGDVEIDEGADVIQVRIKIKAFPMRKEAVVKAVREWLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P776</accession>
    <name>A0A218P776_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_04300</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853857.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P776"/>
    <dbReference type="GeneID" id="33315464"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04300"/>
    <dbReference type="OrthoDB" id="84592at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="253" mass="29030" checksum="8A21E7D737D6148A" modified="2017-09-27" version="1">MTYVAVLANINGNLPALAKALERIEGLKEEGYEIEKYYILGNVVGLFPYPREVLDTLDDLIRNNRVKVIRGEFDQVIAESDPHAEGPDYIDRIEYPDYIKAALKYTWEALGHEGREFIRDLPVYLVDKIGKNDIFGVYGSPLDPFYGKVLPEQPTAYYDAIMRPVKDYEILFVASPRYPVNVMTRYGRVVCPGSVGYPPGKAHRATFALVDVNTLHTKFIEVDYEEEKRLIEERIKREGLPEELVRVLYHGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P793</accession>
    <name>A0A218P793_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_04550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P793"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04550"/>
    <dbReference type="OrthoDB" id="85757at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="122" mass="13935" checksum="5102EB664B06A0CB" modified="2017-09-27" version="1">MKLAIEHKFSLTVYLWGTITGIISGVAAAKMEYGWLLGVALYFVIDRFVMAVIKELPPEIPDERLILRKAFWGWLLFWLYFTMLSYSIMTHFTPQCYSNQSLLYQMVTNGNATVPCNVTPAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7D4</accession>
    <name>A0A218P7D4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_04855</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853950.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7D4"/>
    <dbReference type="GeneID" id="33315575"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04855"/>
    <dbReference type="OrthoDB" id="378845at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="113" mass="12345" checksum="56C1AE2D8ACF0FEA" modified="2017-09-27" version="1">MSKWLVPAVSIGLFLAFSYIAWGVESLISAVILSVILLLSTSSPGRAGIIFYFFIGFLAGFVVAIVIGVKTNATGWESAVLLTLPFIGALVALLYGRRSDYMSWPVFGFRYKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7E5</accession>
    <name>A0A218P7E5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Amino acid permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_04875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853954.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7E5"/>
    <dbReference type="GeneID" id="33315579"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04875"/>
    <dbReference type="OrthoDB" id="99721at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="320"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="398"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="427" mass="45654" checksum="3FBAE9F727EBC17F" modified="2017-09-27" version="1">MDEIKKWTLYLVFLVAGFATGVGSIALFPQFWLQYGITGLAVHLVFLAVLTYVAILEAEVVMKSGYHFVELYTKAANRPAMILSIFAVIAMFLSYYTSNTMLSILGPVLGTGTVARLVAKILMFALVFLILTRAKERTFTIMALGSVFFFVAILVTAVAFKTQIPENATFLGMAKHMLVARQGITLAMVRAAAERAIYGVGLGMAFYLMLGSFINERFNAKVIIGTGVLVQLLIGVMSTIVVVYGLAPTSPERLLTYVYGGEEAALQLLGDLPDILADYQSLLVLIAISGFLAGLTSLLPTAEVGLQIIEPIMGISRTKAATYLVGISLLIGIFDSPPTIADMVLKAVVAATFFTPIFELYPVVREKLSAPAMVAVGVAALVFVVGGLYAFFGVFKAGGVYIVSGILAALIILFGLIGDSLMPQSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7J1</accession>
    <name>A0A218P7J1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable glycine dehydrogenase (decarboxylating) subunit 1</fullName>
            <ecNumber evidence="3">1.4.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Glycine cleavage system P-protein subunit 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Glycine decarboxylase subunit 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Glycine dehydrogenase (aminomethyl-transferring) subunit 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gcvPA</name>
        <name evidence="5" type="ORF">A3L08_05000</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[glycine-cleavage complex H protein] + glycine + H(+) = N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[glycine-cleavage complex H protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:24304"/>
            <dbReference type="Rhea" id="RHEA-COMP:10494"/>
            <dbReference type="Rhea" id="RHEA-COMP:10495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="1.4.4.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">The glycine cleavage system is composed of four proteins: P, T, L and H. In this organism, the P 'protein' is a heterodimer of two subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GcvP family. N-terminal subunit subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.4.4.2" evidence="3"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853975.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7J1"/>
    <dbReference type="GeneID" id="33315604"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05000"/>
    <dbReference type="OrthoDB" id="17655at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004375">
        <property type="term" value="F:glycine dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00613">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00712">
        <property type="entry name" value="GcvPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023010">
        <property type="entry name" value="GcvPA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049315">
        <property type="entry name" value="GDC-P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020581">
        <property type="entry name" value="GDC_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001696">
        <property type="entry name" value="PRK00451.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42806">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM P-PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42806:SF1">
        <property type="entry name" value="GLYCINE DEHYDROGENASE (DECARBOXYLATING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02347">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006815">
        <property type="entry name" value="GcvPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycine cleavage system P-protein N-terminal" evidence="4">
        <location>
            <begin position="3"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00049026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="453" mass="50560" checksum="D4D304707DC9C20E" modified="2017-09-27" version="1">MGRHYIPNSAHKEEMLKEIGFSAIEDLFSDVPKGMVKEFDLPEGKSEYEVFTEMNEILGKNKTVLEMPSFLGAGTYFHYVPAHVKYIIERSEFLTAYTPYQPEISQGMLQALFEYQSMMAELYGLPIVNSSMYDWGTAIAEAALMTVRLHRGKRKRFLILKALSPEKKAVIRTYTRGADLEIVEVPWDEKGQLDLEKLKEEVKDSAGVYVEMSNFFGLLEENVREIGEIAHEAGAYFVVGADPTMLGVFEAPGELGADIAVGEASYLGSPMNFGGPRAGVFAVKNDRSLIRQMPGRIIGMSKDADGKRAFVMTLQTREQHIRRAKATSNICSNEALVAVAAAIHLATLGPKGLAELGEIILKNTAYLKKRLSEVGEVPFEGIYFKDVPVRFEIPYDIIHERLLERGIHGGYYLGKHFPELGETALFAATETTRKEWVDRLVEALGEIISEAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7M3</accession>
    <name>A0A218P7M3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cysteine desulfurase</fullName>
            <ecNumber evidence="3">2.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_05365</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(sulfur carrier)-H + L-cysteine = (sulfur carrier)-SH + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:43892"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="2.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="3"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854037.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7M3"/>
    <dbReference type="GeneID" id="33315677"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05365"/>
    <dbReference type="OrthoDB" id="5817at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006534">
        <property type="term" value="P:cysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06453">
        <property type="entry name" value="SufS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010970">
        <property type="entry name" value="Cys_dSase_SufS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016454">
        <property type="entry name" value="Cysteine_dSase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586">
        <property type="entry name" value="CYSTEINE DESULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586:SF8">
        <property type="entry name" value="CYSTEINE DESULFURASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005572">
        <property type="entry name" value="NifS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="7">
        <location>
            <begin position="18"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="399" mass="44413" checksum="6B9F10D1AEC94B81" modified="2017-09-27" version="1">MKIPDDVRKDIPLTSEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATHKYEESRKVVADFINAEFEEVVFTKNTSESLNLVALGLEHLFKPGDKIVTTPYEHHSDLLPWQRLAKKKGLKLEFIEGDDEGNLDLSDAEKKIRGAKLVAVQHVSNALGVIHEVEELGKLAKEEGAIFVVDAAQSAGHMEVDVRKLNADFLAFSGHKGPMGPTGIGVLYINREFFDVFEPPLIGGGTIEDVDFDGYKLTEPPERFEAGTPNIGGAIGLAAGIRYIQRIGLDKIERQEHKLVKRITEGLDELEVPWYGPRNLKKHAGVVSFNVPPLHPHDVAAILDGRNIMVRSGHHCALPVMKKLGINGTVRASFHVYNSVEEVETFLGVMEELVKSLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Y4</accession>
    <name>A0A218P7Y4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Translation initiation factor 5A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Hypusine-containing protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 11">eIF-5A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">eif5a</name>
        <name evidence="13" type="ORF">A3L08_05820</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Functions by promoting the formation of the first peptide bond.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the eIF-5A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854123.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Y4"/>
    <dbReference type="GeneID" id="33315768"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05820"/>
    <dbReference type="OrthoDB" id="23689at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045901">
        <property type="term" value="P:positive regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045905">
        <property type="term" value="P:positive regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04467">
        <property type="entry name" value="S1_aIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000038">
        <property type="entry name" value="Translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000334">
        <property type="entry name" value="Translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00085">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001884">
        <property type="entry name" value="IF5A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048670">
        <property type="entry name" value="IF5A-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020189">
        <property type="entry name" value="IF5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019769">
        <property type="entry name" value="Trans_elong_IF5A_hypusine_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022847">
        <property type="entry name" value="Transl_elong_IF5A_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00037">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003076">
        <property type="entry name" value="PRK03999.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11673">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 5A FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01287">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21485">
        <property type="entry name" value="IF5A-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003025">
        <property type="entry name" value="eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01376">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00302">
        <property type="entry name" value="IF5A_HYPUSINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11" id="KW-0385">Hypusine</keyword>
    <keyword evidence="6 11 13" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation initiation factor 5A C-terminal" evidence="12">
        <location>
            <begin position="70"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="modified residue" description="Hypusine" evidence="11">
        <location>
            <position position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="136" mass="15014" checksum="E5FCA3879FB8C9B3" modified="2017-09-27" version="1">MGDKTKVQVSKLKPGRYILIDGEPCRIGNITVSSPGKHGSAKARIEAVGIFDGKVRSIVKPTSAEVEVPIIDKRTAQVIAMTPDTVQIMDMETYELFDVPIETGVADEIKDQLKEGINVEYWETLGRIKIMKLKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Z7</accession>
    <name>A0A218P7Z7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 19 20">Adenosylcobinamide-GDP ribazoletransferase</fullName>
            <ecNumber evidence="5 19 20">2.7.8.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16 19">Cobalamin synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15 19">Cobalamin-5'-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">cobS</name>
        <name evidence="21" type="ORF">A3L08_05920</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14 19">Joins adenosylcobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin). Also synthesizes adenosylcobalamin 5'-phosphate from adenosylcobinamide-GDP and alpha-ribazole 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 19">
            <text>alpha-ribazole + adenosylcob(III)inamide-GDP = adenosylcob(III)alamin + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16049"/>
            <dbReference type="ChEBI" id="CHEBI:10329"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="EC" id="2.7.8.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 19">
            <text>alpha-ribazole 5'-phosphate + adenosylcob(III)inamide-GDP = adenosylcob(III)alamin 5'-phosphate + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23560"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57918"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="ChEBI" id="CHEBI:60493"/>
            <dbReference type="EC" id="2.7.8.26"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 19">Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 7/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 19">Cell membrane</location>
            <topology evidence="2 19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the CobS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.26" evidence="5 19 20"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854143.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Z7"/>
    <dbReference type="GeneID" id="33315788"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05920"/>
    <dbReference type="OrthoDB" id="11748at2157"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00238"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051073">
        <property type="term" value="F:adenosylcobinamide-GDP ribazoletransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008818">
        <property type="term" value="F:cobalamin 5'-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00719">
        <property type="entry name" value="CobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003805">
        <property type="entry name" value="CobS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00317">
        <property type="entry name" value="cobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34148">
        <property type="entry name" value="ADENOSYLCOBINAMIDE-GDP RIBAZOLETRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34148:SF1">
        <property type="entry name" value="ADENOSYLCOBINAMIDE-GDP RIBAZOLETRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02654">
        <property type="entry name" value="CobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 19" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 19" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="11 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 19 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="181"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="NCBIfam" id="TIGR00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ASJ06893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="231" mass="25130" checksum="D84DEE359A441CA5" modified="2017-09-27" version="1">MKNILPFLTRIPVKGDFEKARKELWAFPLVALVSSVLPSAILYLELPLANVLALLELYFTIGLLHLDGLADWADGIMVKGDRERKIKAMKDLNTGIAGLFAVVMVLFLQVYSLPLVPFYSIYLAELNSKFAMLLGLSTKRPLGGGLGTYFMEGMNGKQLATGTTLYALLYLPVALYNPPAVIGLAGLLFAAYVIKLSLENFGGINGDCLGAIAEITRAGTLLVLAFARWWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P807</accession>
    <name>A0A218P807_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">KEOPS complex Pcc1-like subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CTAG/PCC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854162.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P807"/>
    <dbReference type="GeneID" id="33315810"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06035"/>
    <dbReference type="OrthoDB" id="8982at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015419">
        <property type="entry name" value="CTAG/Pcc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053619">
        <property type="entry name" value="KEOPS_CTAG/PCC1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040853">
        <property type="entry name" value="KEOPS_Pcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011470">
        <property type="entry name" value="PRK14887.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09341">
        <property type="entry name" value="Pcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="94" mass="10714" checksum="C34ACF57856C5D8F" modified="2017-09-27" version="1">MGLQGGLGDKGAEETWPIEGVIELSFPDEETARVVYESVLYEHESVPYRRSRIEFLRDGKKVVIRFLAKDNSALRGTLNSYLRWIKVAIDSLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P853</accession>
    <name>A0A218P853_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UDP-N-acetyl-D-mannosamine dehydrogenase</fullName>
            <ecNumber evidence="2">1.1.1.336</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">UDP-ManNAc 6-dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_06265</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>UDP-N-acetyl-alpha-D-mannosamine + 2 NAD(+) + H2O = UDP-N-acetyl-alpha-D-mannosaminouronate + 2 NADH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:25780"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:68623"/>
            <dbReference type="ChEBI" id="CHEBI:70731"/>
            <dbReference type="EC" id="1.1.1.336"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.336" evidence="2"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854203.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P853"/>
    <dbReference type="GeneID" id="33315856"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06265"/>
    <dbReference type="OrthoDB" id="372050at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089714">
        <property type="term" value="F:UDP-N-acetyl-D-mannosamine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053627">
        <property type="entry name" value="UDP-sugar_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028359">
        <property type="entry name" value="UDP_ManNAc/GlcNAc_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03026">
        <property type="entry name" value="NDP-sugDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040825">
        <property type="entry name" value="UDPMaNacDH_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43491">
        <property type="entry name" value="UDP-N-ACETYL-D-MANNOSAMINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43491:SF2">
        <property type="entry name" value="UDP-N-ACETYL-D-MANNOSAMINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500136">
        <property type="entry name" value="UDP_ManNAc_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000124">
        <property type="entry name" value="UDPglc_GDPman_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52413">
        <property type="entry name" value="UDP-glucose/GDP-mannose dehydrogenase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="UDP-glucose/GDP-mannose dehydrogenase C-terminal" evidence="10">
        <location>
            <begin position="322"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="428" mass="47091" checksum="D56DA05483723543" modified="2017-09-27" version="1">MEGGIEKRTAEIAVIGLGYIGLPTAIMFANAGFNVTGYEIRKEVVDKINSGEAHIVEPEIDELLKNAVESGRLRATSDPEDIRGKDVYIICVQTPLNEDKTPNLSYLESAVKTVAKAMKRGSLIVIESTVPPLTTVKMAKLIEEITGLKAGEDFYMVHAPERVMPGRIFKELVYNSRIFGGITPESAELAEKLYRSFVKGQTFKTNSTVSEVVKLMENTFRDVNIALANEFAYLAHQYGIDAFEAIELANTHPRVKIHVPGIGVGGHCLPKDPHLLVWPARDDFGLIRLAREVNDGMPLLAKDLLFEALKTVNLPPEKAVVAVLGLAYKGDSDDTRNSPAKAFVEAVEEDVAEVRTYDPFVEGTHGSIEDVLEGADAAVIATDHSEFKSLNWEELGKLMRTRIIIDGRHVVEMPPEGFIFKGIGRGEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P856</accession>
    <name>A0A218P856_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_06310</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_198362097.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P856"/>
    <dbReference type="GeneID" id="33315865"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06310"/>
    <dbReference type="OrthoDB" id="2305at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.280">
        <property type="entry name" value="Cobalamin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051198">
        <property type="entry name" value="BchE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034466">
        <property type="entry name" value="Methyltransferase_Class_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43409">
        <property type="entry name" value="ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43409:SF16">
        <property type="entry name" value="SLR0320 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01123">
        <property type="entry name" value="methyltransferase_(Class_B)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="B12-binding" evidence="6">
        <location>
            <begin position="21"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="202"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="476" mass="55388" checksum="2AC34D4E788D5BDD" modified="2017-09-27" version="1">MKVFMFNPPTGLYIRGEDRCQVPVKGLTATAPRPPIDFAQIAGILKEWYGVRDIYIKDYPVEGGTFKDLYADFNRIQPDYVIISTTTPTILKDAQLVKQLKEDFPETIFVLKGAHFYTTGEDIISRYQVDFAVYGETDIVASELIGKLENGVSPYNIRGIIFYDHNAQRAVRTPPREFFEPLDDLPFPDRSLLRNELYIRPDTGEPQTTIQSHRGCPFKCTYCLSRVVYGNKVRFRSPQNVVDEIEHVYEKFGIRNFFFRADTFTINKKWVIELSKEIIERGLHEKIQWVTNSRVDTIDEERVKWMKKAGCWLVSLGIESGNQEILNRIKKGITLDQARNAVKILKEHGIKTYLFFMLGFPWESRKEVEETIQFAKELDGDFYEFHVLVPFPGTEIYDEIVKLGLLVVDDLTGYDHSKPAIRTLHLTPEEIEELRERAIRETYLSTRYLKKKAIEMLKQPRLILPYAKYGLRLLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P872</accession>
    <name>A0A218P872_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NTP pyrophosphohydrolase MazG-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854230.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P872"/>
    <dbReference type="GeneID" id="33315886"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06415"/>
    <dbReference type="OrthoDB" id="85269at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1080">
        <property type="entry name" value="MazG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004518">
        <property type="entry name" value="MazG-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012359">
        <property type="entry name" value="MazG-related_YpjD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047046">
        <property type="entry name" value="YpjD/YvdC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42692">
        <property type="entry name" value="NUCLEOTIDE PYROPHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42692:SF1">
        <property type="entry name" value="NUCLEOTIDE PYROPHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03819">
        <property type="entry name" value="MazG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029904">
        <property type="entry name" value="UCP029904_pph"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101386">
        <property type="entry name" value="all-alpha NTP pyrophosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTP pyrophosphohydrolase MazG-like" evidence="1">
        <location>
            <begin position="23"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="104" mass="12042" checksum="C4AA07F6E9A4618F" modified="2017-09-27" version="1">MNEHQAKVDELVKELGGYWKPFEMLAALVEEVGELADELLKVEGVKGERSPEHLKEELGDVLFALTCIANYYGVDILEALAESVEKYRIRDKNRWEERHQVVQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8A2</accession>
    <name>A0A218P8A2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phage ABA sandwich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06725</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854286.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8A2"/>
    <dbReference type="GeneID" id="33315948"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06725"/>
    <dbReference type="OrthoDB" id="98562at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="93" mass="10605" checksum="5826D46B2D368C37" modified="2017-09-27" version="1">MNELFERVKEEYGVEIRDENDMTNAWKLVEALKEKGWVVYIITAKGREQVDAWHPSFGSLFAQFGENPNFGSVLEGICNIALLVKELEKNGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8A7</accession>
    <name>A0A218P8A7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06635</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854271.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8A7"/>
    <dbReference type="GeneID" id="33315930"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06635"/>
    <dbReference type="OrthoDB" id="350705at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1640.10">
        <property type="entry name" value="inorganic pyrophosphatase (n-terminal core)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051319">
        <property type="entry name" value="Oligoribo/pAp-PDE_c-di-AMP_PDE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47618">
        <property type="entry name" value="BIFUNCTIONAL OLIGORIBONUCLEASE AND PAP PHOSPHATASE NRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47618:SF1">
        <property type="entry name" value="BIFUNCTIONAL OLIGORIBONUCLEASE AND PAP PHOSPHATASE NRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RCK N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="487" mass="53991" checksum="50C39B9487973DD2" modified="2017-09-27" version="1">MRVLVLGGGALGRSVAESLKGEFDVTIIEKDEIRAQALEESGFTVIKGDFSYTATLLKAGIDRAELVIITTMDLDTIRKTVYVIRTNNREVPILTVLPDDVNLEDLVSQINEEYEAEVKIDYTISPRSALRDAIVKTVEITGEKKSANLLVRKLKELKAQDDSLLILMHDNPDPDAIASAAALSVIAQTLGFKTQVAYGGEIAHHENRAFVNLLGVELRKVSRGSYELKRYPFIALVDCQPNGNLTTLEQEDYEKIKVIIDHHQILQHLQELIPEDAFLDIRPEVHSSSSILAEYLRMLNISLSPLLATALFYGIYIDTKKFSKLSHVDLKAIEFLAGKVDYELLDKIEHPDISTETAEILARAILNRRIYKNVVISNVGFITNRDAIAESADFLLRLEGITTVLVFGIVDDRIEMSARTRDVRVNIGAVLREAFGDIGSGGGHSQSGGARVPLGIFKLAKDKNSLLRLVEEAITEKFLEALKVKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8B2</accession>
    <name>A0A218P8B2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cation:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854256.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8B2"/>
    <dbReference type="GeneID" id="33315913"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06550"/>
    <dbReference type="OrthoDB" id="19138at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005133">
        <property type="entry name" value="PhaG_MnhG_YufB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01300">
        <property type="entry name" value="CPA3_mnhG_phaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34703">
        <property type="entry name" value="ANTIPORTER SUBUNIT MNHG2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34703:SF1">
        <property type="entry name" value="ANTIPORTER SUBUNIT MNHG2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03334">
        <property type="entry name" value="PhaG_MnhG_YufB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="110" mass="11440" checksum="F49E7673BFAFA634" modified="2017-09-27" version="1">MILSLIGTLLLIVGAVCDIFGAIGMHRFGNFYLRLHAATVGTVGGKFYPLIGVGLIALDRGLLPIAGVAFLSAFTLLITTSVGSHALAYAAERAGVVKIEHDELGGDEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8B4</accession>
    <name>A0A218P8B4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Metalloprotease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_06350</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854219.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8B4"/>
    <dbReference type="GeneID" id="33315873"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06350"/>
    <dbReference type="OrthoDB" id="19110at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06160">
        <property type="entry name" value="S2P-M50_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044838">
        <property type="entry name" value="EGY1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31412">
        <property type="entry name" value="ZINC METALLOPROTEASE EGY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31412:SF0">
        <property type="entry name" value="ZINC METALLOPROTEASE EGY1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 10" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="304"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="347"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="397"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M50" evidence="9">
        <location>
            <begin position="179"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="418" mass="45489" checksum="1CAA8DD47E986311" modified="2017-09-27" version="1">MPRGIYECVNCGHREVIDSNEPLLERACPACGGDMVLVGFEAEMLEEKAPEWARIGLFLPPEVEARVHSFYSVEPRGSEGNVFVFEVKEILEPDFEKVLRELEELGYWAALKKRGGKVLLYVFPAGEVKPDNPWLPWAFLMATIITTFLAGYWLALGYIDLINRYNLPGIRNPYVNALAFSISVMAILGTHELGHKIAAAYHGVRATMPYFIPFPSMLGTLGAVIRVKSPVPTRNAAIDLGVSGPLAGILVAIPVTAIGLRLSAVVPSSMVPQTGEDLYMGTNLFFGVLEKLVLGIGSGEDYTVFLHPVAIAGWVGILVTFLNLIPAAQLDGGHIARAFMGEKAHRYFTFAIAFGLLLMSYLWSGWLIWGLLVLFIGSAGNPGALDEVSPVSTGRKLLAIVAVLIFILCATPVPLYVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8B7</accession>
    <name>A0A218P8B7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_06625</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854269.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8B7"/>
    <dbReference type="GeneID" id="33315928"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06625"/>
    <dbReference type="OrthoDB" id="29208at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050638">
        <property type="entry name" value="AA-Vitamin_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32322:SF2">
        <property type="entry name" value="EAMA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32322">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="109"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="215"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="271"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="6">
        <location>
            <begin position="6"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="6">
        <location>
            <begin position="153"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="289" mass="31080" checksum="E2F4616025143416" modified="2017-09-27" version="1">MSTLILGILAALISALSWASSTILLKVGMRDRTPVAVNIIRLYIVAVAFAVIFLVNGTFSQVLALSPELLAIAFISSMFGFVIGDYFYLHALNMMGVSRTVPITSTYPLWVILWAFLFLGREISPQILLGAGLVVSAIILVRRAEEEERADPKGFIFAVLAPISWSLAIFTMDWLTGYIDVLALAGLRMMSAAIGVSIFLPKYLPELRRVTLRELAILAGAALSGLLVGQYFFVYSVSSVGSQIAAPVSAINPIIASALAILLLKEPPNRKILEGLLLAVVGVILISTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8C5</accession>
    <name>A0A218P8C5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">2-oxoglutarate synthase subunit KorA</fullName>
            <ecNumber evidence="4">1.2.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">2-ketoglutarate oxidoreductase alpha chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">2-oxoglutarate-ferredoxin oxidoreductase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_06780</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2 oxidized [2Fe-2S]-[ferredoxin] + 2-oxoglutarate + CoA = succinyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17297"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="EC" id="1.2.7.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterotetramer of the KorA, KorB, KorC and KorD subunits.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.3" evidence="4"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854295.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8C5"/>
    <dbReference type="GeneID" id="33315959"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06780"/>
    <dbReference type="OrthoDB" id="31112at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000022">
        <property type="entry name" value="2-oxoglutarate ferredoxin oxidoreductase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000013">
        <property type="entry name" value="Ferredoxin oxidoreductase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050722">
        <property type="entry name" value="Pyruvate:ferred/Flavod_OxRd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006412">
        <property type="entry name" value="PRK08659.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154:SF14">
        <property type="entry name" value="2-OXOGLUTARATE SYNTHASE SUBUNIT KORA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154">
        <property type="entry name" value="PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate flavodoxin/ferredoxin oxidoreductase pyrimidine binding" evidence="8">
        <location>
            <begin position="34"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate:ferredoxin oxidoreductase core" evidence="9">
        <location>
            <begin position="294"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00052359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00064882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00066947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00071398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00076968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00079587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ07045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="396" mass="43842" checksum="B618F4976006270F" modified="2017-09-27" version="1">MIIRGDEPEQVRLIKKLYKPGNYFMQGNEAVAYGAIFAGCRFYAGYPITPSSEIAETMARELPKLGGYYLQMEDEIGSIAAMVGASWTGFKVMTATAGPGFSLMQENLGYAVMTETPLVLVDVQRSGPSTGQATKGAQGDFFQARWGTHGDHPIVAVSPTSGQDAFWETIRAFNIAEKLRTPVVVLFDGVLAHTREQIRIPDVEEVEIAYRKLPANEEEARLPFGDPHGDGVPPMPLFGHGYFTHVTGSTHKENGLRDVYTPEVHDRLVRRIHRKIEQNRDVYEKYEEHFADDAEILVVSWGVTARPALGAVLKAREEGIKAGLFVPKTVHPFPGERMKQLGKKVRAVLVAEMNLGQMILEVQRFVNDDVLLKGVNKIGGVPLTVEEILREIRGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8L3</accession>
    <name>A0A218P8L3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein pelota homolog</fullName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pelA</name>
        <name evidence="11" type="ORF">A3L08_07220</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">May function in recognizing stalled ribosomes, interact with stem-loop structures in stalled mRNA molecules, and effect endonucleolytic cleavage of the mRNA. May play a role in the release non-functional ribosomes and degradation of damaged mRNAs. Has endoribonuclease activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">The N-terminal domain has the RNA-binding Sm fold. It harbors the endoribonuclease activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the eukaryotic release factor 1 family. Pelota subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854377.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8L3"/>
    <dbReference type="GeneID" id="33316047"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07220"/>
    <dbReference type="OrthoDB" id="31300at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071025">
        <property type="term" value="P:RNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.870:FF:000002">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000059">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.60:FF:000005">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.870">
        <property type="entry name" value="Pelota, domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01853">
        <property type="entry name" value="PelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038069">
        <property type="entry name" value="Pelota/DOM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023521">
        <property type="entry name" value="Pelota_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058547">
        <property type="entry name" value="Pelota_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004405">
        <property type="entry name" value="TF_pelota"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00111">
        <property type="entry name" value="pelota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853">
        <property type="entry name" value="PELOTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853:SF0">
        <property type="entry name" value="PROTEIN PELOTA HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26356">
        <property type="entry name" value="Pelota_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159065">
        <property type="entry name" value="Dom34/Pelota N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="10">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ07126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="357" mass="40727" checksum="5CCB5747EC24762A" modified="2017-09-27" version="1">MQIIHQDVKEGKVKVKAETLDDLWHLYHIIDPGDIVYAKTLRKQSQRSDSLRAEKVEVIPVFLGVKAEKINFHKFANQVRVTGPIVYASREDVPLSKYHTIAIEEGTVVTIEKPRWKEHHIERLKEAVEASKRARVMIVVIDDGEADMALVREYGVEILNSIRHNLGGKRYNTDRESEEKKFFHDVAKTMEEVMNRENVEKAIVAGPGFVKEDFYKFLQENYPELAKKVVIEDTSVTGRTGIYEVIKRGTVERVYHENRVAKEVQLVEKVLENIARNNGLAAYGLKEVEEAVNYGAVETLLVLDELLKGEHREKIEELMDAVRYSRGEVVVVSSEHEGGEKLKALGGLAALLRFRVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8M7</accession>
    <name>A0A218P8M7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bifunctional protein GlmU</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_07255</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854384.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8M7"/>
    <dbReference type="GeneID" id="33316054"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07255"/>
    <dbReference type="OrthoDB" id="15372at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="3">
        <location>
            <begin position="2"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="4">
        <location>
            <begin position="257"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="413" mass="47054" checksum="EDA54127A66D2558" modified="2017-09-27" version="1">MKAVILAGGFGTRLRPLSSTRPKPMVPVLGKPNLQYLLESLEKIPEIDEIILSVHYMRGEIREFIDEKMADYPKDIRFVNDPMPLETGGALKNVEDYVDEDFLVIYGDVFTNFDFRELIEAHRKNEGLVTVAVTKVYDPERYGVVETDEEGKVTHFEEKPHRPKTNLVDAGIYMVNKKVLEEIPKNREVYFEREVLPKFVSKGEVYAYRMARDYYWIDLGTPEDLFYAHQIAMDEIAKDNGYMTIKETAEVPDDVEIQGPVYIDEGAKIGHGVKIKSYTYIGPNTIVEDKAYFKRAILIGSDIVKSKSEIKDSILGEGVVVGKNVILKENAVVGDYAKIYDNLVIYGAKVLPWKKVEEYEAFIKIKLDPTKVKPGVTPERCPLGLPECIYTKFKAIAGEKPPCDECIENQWLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8N6</accession>
    <name>A0A218P8N6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Elongation factor 2</fullName>
            <shortName evidence="10">EF-2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 12" type="primary">fusA</name>
        <name evidence="12" type="ORF">A3L08_07375</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854407.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8N6"/>
    <dbReference type="GeneID" id="33316078"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07375"/>
    <dbReference type="OrthoDB" id="6290at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01681">
        <property type="entry name" value="aeEF2_snRNP_like_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01885">
        <property type="entry name" value="EF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16268">
        <property type="entry name" value="EF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16261">
        <property type="entry name" value="EF2_snRNP_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01514">
        <property type="entry name" value="Elongation_Factor_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000009">
        <property type="entry name" value="116 kDa U5 small nuclear ribonucleoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000110">
        <property type="entry name" value="Elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000010">
        <property type="entry name" value="Elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000684">
        <property type="entry name" value="Elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000002">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00054_A">
        <property type="entry name" value="EF_G_EF_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004543">
        <property type="entry name" value="Transl_elong_EFG/EF2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00490">
        <property type="entry name" value="aEF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF3">
        <property type="entry name" value="ELONGATION FACTOR-LIKE GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10 12" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="19"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="28"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="94"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="148"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Diphthamide" evidence="10">
        <location>
            <position position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ07155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="732" mass="81840" checksum="542736F60D4F3293" modified="2017-09-27" version="1">MGRREEMIAKIKELMTQPERIRNMGIAAHIDHGKTTLSDNLLAGAGMISEELAGKQLVLDFDEQEQARGITINAANVSMVHNYEGNDYLINLIDTPGHVDFGGDVTRAMRAIDGAIIVVDAVEGVMPQTETVLRQALREYVKPVLFINKVDRLIKELKLGPNDILQRFAKIITDVNRLIKKYAPDEFKNQWMVKVEDGSVAFGSAYYNWALSVPYMKKTGVSFKDIVELTNSGDLKTLRQKAPLHVVVLDMVVKHLPNPLEAQKYRIPHLWRGDVNSDVGQAMLKCDPKGPMTMVVTKIILDKHAGEVSTGRVWSGTVKTGQEVYLINAKRKSRIQQVGIYMGPERVNMEAVPAGNIVAVTGLRDAMAGETVSTEQIEPFEALHYASEPVVTVAIEAKNVKDLPKLIEALRQLAKEDPTLHVKIDEETGQHLLSGMGELHLEVKLVKLKEDWKLDVEVSPPIVVYRESVSKVSPIVEGKSPNKHNRFYITVEPLPDEIYQAIREGEIPEGRPKDPKAVAKKLAELGMDYEVAKGIVDVYQGNIFLDNTKGIQYLNEVMDLLVDGFHMAMDEGPLAKEPVMKVMVRLHDAKIHEDNVHRGPAQIYPAIRTAIHCAMMKAQPILYEPYQKVIINVPYEYMGAVSREINQRRGQLIDMRQEGEVMIIIAEAPVAEMFGFAGAIRGATSGRALWSTEHAGFKRVPNELAQQIIRQIRQRKGLDPNPPKEQDVCPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8P2</accession>
    <name>A0A218P8P2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA (pseudouridine(54)-N(1))-methyltransferase</fullName>
            <ecNumber evidence="6">2.1.1.257</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">trmY</name>
        <name evidence="7" type="ORF">A3L08_07260</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Specifically catalyzes the N1-methylation of pseudouridine at position 54 (Psi54) in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>pseudouridine(54) in tRNA + S-adenosyl-L-methionine = N(1)-methylpseudouridine(54) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55292"/>
            <dbReference type="Rhea" id="RHEA-COMP:14140"/>
            <dbReference type="Rhea" id="RHEA-COMP:14141"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
            <dbReference type="EC" id="2.1.1.257"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the methyltransferase superfamily. TrmY family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.257" evidence="6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854385.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8P2"/>
    <dbReference type="GeneID" id="33316055"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07260"/>
    <dbReference type="OrthoDB" id="27492at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18087">
        <property type="entry name" value="TrmY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00587">
        <property type="entry name" value="tRNA_methyltr_TrmY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007158">
        <property type="entry name" value="TrmY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002560">
        <property type="entry name" value="PRK02135.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40703">
        <property type="entry name" value="TRNA (PSEUDOURIDINE(54)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40703:SF1">
        <property type="entry name" value="TRNA (PSEUDOURIDINE(54)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04013">
        <property type="entry name" value="Methyltrn_RNA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0819">tRNA processing</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="203" mass="22672" checksum="219865540F630127" modified="2017-09-27" version="1">MRTFIIKANRAHTKADFSLTDLPGTSGRIDLLCRFLNSAFLLSHGFRKNVRVWLSLYGPPNPPKAIRFEGQRMRPKTLNPDELSTAKLIIKALKAGENLREPGKELEVLPGIYVSKLTFEDIVRRTLRGSALYYLHEEGKPIEKVHFPANVAFVLGDHEGLSREDEAFLAGIAEKVSVGKKSYLASHVVAYVNIHLDSLTPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8S4</accession>
    <name>A0A218P8S4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_07460</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HypE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854419.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8S4"/>
    <dbReference type="GeneID" id="33316095"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07460"/>
    <dbReference type="OrthoDB" id="31494at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06061">
        <property type="entry name" value="PurM-like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011854">
        <property type="entry name" value="HypE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30303:SF4">
        <property type="entry name" value="HYDROGENASE EXPRESSION_FORMATION PROTEIN HYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30303">
        <property type="entry name" value="HYDROGENASE ISOENZYMES FORMATION PROTEIN HYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005644">
        <property type="entry name" value="Hdrgns_mtr_HypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="2">
        <location>
            <begin position="36"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="3">
        <location>
            <begin position="152"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="330" mass="35185" checksum="20180BD90A1704CE" modified="2017-09-27" version="1">MLPPGKIPPEKLKELVFSRLGAPGERVLIGADLGIDAAAIDFGDKVLVASTDPITGAEENIGFYAVHVNANDVATFGARPKWFLVSILLPEGSDERTLEQIMEDLHASASKLGIAIVGGHTEVTPGLKRPIVVGTMLGEVEKGKLVTSNGAKPGDAIILTKWAGLEGTSIIASERSEELEREFGKDFVERAKSFIDMISVVEDALTASEVGVHAMHDPTEGGIANGLHEMADAAGLGFAVYADKIPIRDETRRICEFYGLTPLALISSGALMISAPGEKAGKVVAALMEKGINASVVGEFVKDPERRVLIENGEERPLERPESDELWKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8U4</accession>
    <name>A0A218P8U4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protease HtpX homolog</fullName>
            <ecNumber evidence="11">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">htpX</name>
        <name evidence="13" type="ORF">A3L08_07600</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the peptidase M48B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854444.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8U4"/>
    <dbReference type="SMR" id="A0A218P8U4"/>
    <dbReference type="GeneID" id="33316123"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07600"/>
    <dbReference type="OrthoDB" id="28389at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07336">
        <property type="entry name" value="M48B_HtpX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00188">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050083">
        <property type="entry name" value="HtpX_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022919">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43221">
        <property type="entry name" value="PROTEASE HTPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43221:SF2">
        <property type="entry name" value="PROTEASE HTPX HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 11 13" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M48" evidence="12">
        <location>
            <begin position="68"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ07193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="290" mass="32105" checksum="99560F2062F492D4" modified="2017-09-27" version="1">MGLLMWLRTGLLMAILTGLLMGIGYLFGGPNVAFIMFLFSLFFNFLTYWYSDRIVLSWYNARLVDEYEAPELYAIVRNLAERAGLPMPKVAIVPTETPNAFATGRDPKHAVVAVTTGLLKILNRDELEGVIGHELTHIKNRDMLIGTFAAAMAGAIMQLAYWARWIAIFGGFSRDRDEGGDIIAAILVAVLAPIAAMLIQAAVSRSREFLADEGGARISGKPHALASALMKIEQAVRYRPMRNGNPATAHMFIVNPFRGMSIANLFSTHPPTEARIERLRKIAEEMGIYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8W6</accession>
    <name>A0A218P8W6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07735</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854464.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8W6"/>
    <dbReference type="GeneID" id="33316149"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07735"/>
    <dbReference type="OrthoDB" id="165911at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007050">
        <property type="entry name" value="HTH_bacterioopsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="Sigma3 and sigma4 domains of RNA polymerase sigma factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH bat-type" evidence="1">
        <location>
            <begin position="162"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="218" mass="24955" checksum="1E4621D5812D375D" modified="2017-09-27" version="1">MKRIKFSIPISLDYFNAFRSFLEAVEWGYGDTYFLLGSDVVKLVEVKFKEGVKPEEVLGELLKIPYVKDAKLIPKNDHYLLYVRANLLHASLPENVSRFFEVQKRGMVVFEKGTFSTEGFYLYVVCEEGLLGDVISAVKEVYGARVVSVEDYTPGENPLLKLTGRQFEVLLLAYKSGYFDNPRRVTLRELSQMLGLSPSTVKEHLRKGLKRVLEEVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Y8</accession>
    <name>A0A218P8Y8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleotide pyrophosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07855</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854487.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Y8"/>
    <dbReference type="GeneID" id="33316176"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07855"/>
    <dbReference type="OrthoDB" id="49823at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11535">
        <property type="entry name" value="NTP-PPase_SsMazG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1080">
        <property type="entry name" value="MazG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004518">
        <property type="entry name" value="MazG-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42702">
        <property type="entry name" value="NUCLEOTIDE PYROPHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42702:SF1">
        <property type="entry name" value="REGULATORY PROTEIN FOR BETA-LACTAMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03819">
        <property type="entry name" value="MazG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101386">
        <property type="entry name" value="all-alpha NTP pyrophosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTP pyrophosphohydrolase MazG-like" evidence="1">
        <location>
            <begin position="25"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="97" mass="11208" checksum="9FE9FE210357F272" modified="2017-09-27" version="1">MEIRGFQEMIRDIYFHKDSKRGVDKTFLWFVEEVGELSEAIRKNDREAMEEEFADVLAWLASLANLLGIDIEEAAKKKYPGACPYCGKNPCECEEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Z9</accession>
    <name>A0A218P8Z9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Aspartate racemase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_07850</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aspartate/glutamate racemases family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854486.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Z9"/>
    <dbReference type="GeneID" id="33316175"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07850"/>
    <dbReference type="OrthoDB" id="269620at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047661">
        <property type="term" value="F:amino-acid racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1860">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015942">
        <property type="entry name" value="Asp/Glu/hydantoin_racemase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001920">
        <property type="entry name" value="Asp/Glu_race"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004380">
        <property type="entry name" value="Asp_race"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00035">
        <property type="entry name" value="asp_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21198:SF7">
        <property type="entry name" value="ASPARTATE-GLUTAMATE RACEMASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21198">
        <property type="entry name" value="GLUTAMATE RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01177">
        <property type="entry name" value="Asp_Glu_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53681">
        <property type="entry name" value="Aspartate/glutamate racemase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="226" mass="25526" checksum="542FD358CBBC5F1C" modified="2017-09-27" version="1">MKKIGIIGGTTPESTCYYYQNYIKISREKFGPFTFPELIVYSIDFEEFKNNPRGWEGRKEMLINAARALERAGAEIIAMSANTPHIVFPEVQKAVNVPMVSIIDALIEEMKRRGVKKVLLLGTKTTMTADFYKNALREAGFEVITPSEEEIDEIDRIIFSELAFENLKSKPYLIELVERYAREKGIEGVILGCTELPLAIKPGDVSVEVFDTAAIHMRKLIELAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P928</accession>
    <name>A0A218P928_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thiol reductase thioredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854516.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P928"/>
    <dbReference type="GeneID" id="33316207"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08010"/>
    <dbReference type="OrthoDB" id="35385at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050620">
        <property type="entry name" value="Thioredoxin_H-type-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10438">
        <property type="entry name" value="THIOREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10438:SF468">
        <property type="entry name" value="THIOREDOXIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="101" mass="11815" checksum="73254E15D50C2349" modified="2017-09-27" version="1">MIVEYDGKTDFESGKVVLWFSIPGCPPCRLVESFIEDVSREFSEIRILHVNAEEWNDLVSRFDVLNVPTLIYLKDGREVARQNLIRSKEEVLVRFEELARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P939</accession>
    <name>A0A218P939_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Amino acid permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_07915</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854498.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P939"/>
    <dbReference type="GeneID" id="33316188"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07915"/>
    <dbReference type="OrthoDB" id="103580at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003333">
        <property type="term" value="P:amino acid transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018227">
        <property type="entry name" value="Amino_acid_transport_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32195">
        <property type="entry name" value="OS07G0662800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32195:SF26">
        <property type="entry name" value="TRYPTOPHAN OR TYROSINE TRANSPORTER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03222">
        <property type="entry name" value="Trp_Tyr_perm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="233"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="267"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="343"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="370"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="398" mass="42668" checksum="E6EE6DC47AEADBB7" modified="2017-09-27" version="1">MPVSDGVPRSKVTTSHGHYYAERVALARRKKLRRKATLIQLMRKRKGVAAIRTSTIRVEKAHISKSEALAILIGTQIGAGVLGLPYAASKVGLIPALGVLIGVMLLMLGTAFIVLRFSAEMGGAQMSTIASRTLGKAGGWLMYLSITLMSFGALLAYMAGMGQVFASLFGVSETIGGAIFWVLASLVVYHGLEASGKTELIMSYIMLALFIGVTFMLVPHAKLENGLYADLSGLLSIMGVAIFALGCHTIIPDVYKGLGSYEKTRKVLVLAFLIPTVIYAVFMASFLLVFGRGTPEVATQGLESLYGRAGWLVGNLIPLLAITTSYIGIALAQQSNSEEFVRLRKPIAWALTVVPPAIVYFAGVRNFADVLAFAGDTGDMLAFIVLPVLMWLSAKLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P955</accession>
    <name>A0A218P955_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">AsnC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_08020</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854518.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P955"/>
    <dbReference type="GeneID" id="33316209"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08020"/>
    <dbReference type="OrthoDB" id="33200at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001034">
        <property type="entry name" value="DeoR_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013603">
        <property type="entry name" value="TRASH_TR_C_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019885">
        <property type="entry name" value="Tscrpt_reg_HTH_AsnC-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154">
        <property type="entry name" value="LEUCINE-RESPONSIVE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR AZLB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08394">
        <property type="entry name" value="Arc_trans_TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00519">
        <property type="entry name" value="HTH_ASNC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51000">
        <property type="entry name" value="HTH_DEOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH deoR-type" evidence="5">
        <location>
            <begin position="5"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="5"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="201" mass="23368" checksum="34F76505B60F1768" modified="2017-09-27" version="1">MDVKMDELDLKLIYLLMDDSRLSISELAERLGVSRPTVRARLEKLEKEGVIQGYTVKLNPELLRAHNVVALIVKTDEPEKMNEFEEIIEINRFTSRKYLIKIAVENMEELRKVIEGAGFEVIEIMPILGSVERKNPPKVKVPFKCDYCGKEIVGEPIVYKYRNKVYFFCCPTCLREFRKTRESIEKFKPKEEGSAHEGHSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P969</accession>
    <name>A0A218P969_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Flavodoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_08330</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SsuE family. Isf subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854573.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P969"/>
    <dbReference type="GeneID" id="33316272"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08330"/>
    <dbReference type="OrthoDB" id="73155at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001226">
        <property type="entry name" value="Flavodoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39201">
        <property type="entry name" value="EXPORTED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39201:SF1">
        <property type="entry name" value="FLAVODOXIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00201">
        <property type="entry name" value="FLAVODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="3">
        <location>
            <begin position="3"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="153" mass="17136" checksum="8EB515072BE00D9B" modified="2017-09-27" version="1">MKALVVYYSRDGHTREAARRIAEALGADLDEVIDRKNRKGILGFLRAGYDATRGKTTEIAFEKDPAEYDLVVVGSPVWNGRVTPAIRTYLLKNREKIKKAAFFATCAGRKGKILEQMRELYGGEVIAETIMKRDEIEEGAKEFVEALKSAVHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P992</accession>
    <name>A0A218P992_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854596.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P992"/>
    <dbReference type="GeneID" id="33316296"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08450"/>
    <dbReference type="OrthoDB" id="15119at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="217" mass="25872" checksum="AFE77759F06DD84D" modified="2017-09-27" version="1">MDNQLLMAIIIFLAGIATLQYFRGRKLNLLLMQHYIRSVKDVVKPEDEIYTWLGGYIGFRAEYKINRDNLRKLEYTLTLLPRHSLLYFPISLLTSRHDKLYFVFKPFAQIKREAHLIQKGYYRLKPDIENADLLQREEIEISGKKYEALFEKRRDMEKLKSLIESMSTPRNIKHVALTPKTNVLYIQMKPEPETIEEDVRKMVEFINREIKESPFSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9A8</accession>
    <name>A0A218P9A8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GNAT family acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854577.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9A8"/>
    <dbReference type="GeneID" id="33316276"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08350"/>
    <dbReference type="OrthoDB" id="38613at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="174" mass="20632" checksum="0D1F93B7A4B94E2C" modified="2017-09-27" version="1">MDIERVQRPLELKNELVRFVFRVYQGTGGAYPALEWVEKKPSTDDFEGFKRVYEPFLEFRLGEEFDELYVLRENGRIIGTVALVYNLKGKDVWWVPEEIKNEKTGLIEFFMVDPAYRGKGYGSRLLEFAVERLKELGKEAYVITFPNLEAYSYYMRKGFTKVMDYKEFVVLKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9B0</accession>
    <name>A0A218P9B0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EVE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_08650</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854628.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9B0"/>
    <dbReference type="GeneID" id="33316336"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08650"/>
    <dbReference type="OrthoDB" id="372359at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="155" mass="17923" checksum="2B12AB2CDBAA3C2D" modified="2017-09-27" version="1">MGSLAKYWIETSGPPTIDYIGKILVSPADKGYWKNMEKVSKGDIVYHYITSKGPEPVRRSFIGKSTVKEPAVQYDLDGMLDYVQSIIGENWDSRWEEFAKNRWGDYETFYIVELENFQRFSPPIPLKILKGVFRPTPMYLRSAPMKLVTMLEKGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9B2</accession>
    <name>A0A218P9B2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nucleoside-triphosphatase A3L08_08585</fullName>
            <shortName evidence="4">NTPase</shortName>
            <ecNumber evidence="4">3.6.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Nucleoside triphosphate phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_08585</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Has nucleotide phosphatase activity towards ATP, GTP, CTP, TTP and UTP. May hydrolyze nucleoside diphosphates with lower efficiency.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="EC" id="3.6.1.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the THEP1 NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.15" evidence="4"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854618.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9B2"/>
    <dbReference type="GeneID" id="33316322"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08585"/>
    <dbReference type="OrthoDB" id="52698at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19482">
        <property type="entry name" value="RecA-like_Thep1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00796">
        <property type="entry name" value="NTPase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004948">
        <property type="entry name" value="Nuc-triphosphatase_THEP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010248">
        <property type="entry name" value="PRK13695.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43146">
        <property type="entry name" value="CANCER-RELATED NUCLEOSIDE-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43146:SF1">
        <property type="entry name" value="CANCER-RELATED NUCLEOSIDE-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03266">
        <property type="entry name" value="NTPase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="9"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="104"/>
            <end position="111"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="180" mass="19959" checksum="269B0D694C4EC092" modified="2017-09-27" version="1">MVLRIFVTGPAGVGKTTLVERVAKEVDRWGYIVGGMITQEVRRGGRRVGFKIITLDTGEEGTLASIRGTSHLPGVPFGKYVVHVDEIERVAVPAIKRALVEADLIVIDEIGPMEYKSDEFARVVGEVLKSEKPLLAVVHRKMVDKFRPLGEIHALSFENRNAEFGIILDRIMKELKGIRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9C3</accession>
    <name>A0A218P9C3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_08710</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854638.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9C3"/>
    <dbReference type="GeneID" id="33316348"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08710"/>
    <dbReference type="OrthoDB" id="372921at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="120" mass="13643" checksum="D458D3C64E6512E7" modified="2017-09-27" version="1">MSRRIIVWYEYAEGTVKLALQEHALALNKNGYELIIPVNSKTILEGLNDNIEKKLEEFYLEGHGEIVLIVSPQGDMIGYMFLEEELQQLTQRVSASHSFKKPLQDVLSVTEDVENPLPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9D3</accession>
    <name>A0A218P9D3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Deoxyribonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_08730</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. TatD-type hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854642.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9D3"/>
    <dbReference type="GeneID" id="33316352"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08730"/>
    <dbReference type="OrthoDB" id="26412at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004536">
        <property type="term" value="F:DNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01310">
        <property type="entry name" value="TatD_DNAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018228">
        <property type="entry name" value="DNase_TatD-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015991">
        <property type="entry name" value="TatD/YcfH-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00010">
        <property type="entry name" value="YchF/TatD family DNA exonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46317">
        <property type="entry name" value="HYDROLASE OF PHP SUPERFAMILY-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46317:SF1">
        <property type="entry name" value="HYDROLASE, TATD FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005902">
        <property type="entry name" value="DNase_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01091">
        <property type="entry name" value="TATD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="5"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR005902-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="252" mass="29134" checksum="9644395FC93C2639" modified="2017-09-27" version="1">MIDAHAHVEMFKGKAPEIVEESRKRLKAVVDSITEYRKFHVWKSWEILRPYFGFIQPTLGYAPNEARRGNWEKVGKVEAFIREHAGEIVALGEIGLDFYYAKTEEERRNQREIFHHFLGIAVELDLPVVLHARDAEREVFEAIQRAGVRAYFHSYSGPKELALEIAENGHIIGINTGIDFIPEVREAAEVLPLESIVVETDSPYMSPYKGEKNYPWNVEYAVRRIAEIKGLEFEEIEKTTERNAVRFFGLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9G2</accession>
    <name>A0A218P9G2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Molybdopterin-guanine dinucleotide biosynthesis protein MobA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_08915</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854676.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9G2"/>
    <dbReference type="GeneID" id="33316389"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08915"/>
    <dbReference type="OrthoDB" id="28434at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="198" mass="22634" checksum="DA4695657719B00E" modified="2017-09-27" version="1">MRAVVMAFPEKRWENYTIPINDEPVVKLTEKRLLMSKRIDEVFTIVRKDKLKTYSLHVSNPVPVSARSKMEALLKALPDEPFFLVEGNMPLVMPFLVNYLIGLFYENEPEALIPVWKDGTAEVTHAVYEPDALTDAIEAALAEGHRSMSKITEFLDYESVSIEELSKRNPKVTLSFFRVKSSLDVRFAEQALKDSVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9G7</accession>
    <name>A0A218P9G7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Large ribosomal subunit protein eL8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 11" type="primary">rpl7ae</name>
        <name evidence="11" type="ORF">A3L08_08815</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, the RNA component of RNase P, box H/ACA, box C/D and box C'/D' sRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Part of the 50S ribosomal subunit. Probably part of the RNase P complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854658.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9G7"/>
    <dbReference type="GeneID" id="33316369"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08815"/>
    <dbReference type="OrthoDB" id="25810at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000020">
        <property type="entry name" value="50S ribosomal protein L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00326">
        <property type="entry name" value="Ribosomal_eL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022481">
        <property type="entry name" value="Ribosomal_eL8_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03677">
        <property type="entry name" value="eL8_ribo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00884">
        <property type="entry name" value="RIBOSOMALHS6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 9 11" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 9" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="10">
        <location>
            <begin position="18"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ07413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="123" mass="13436" checksum="DF1BB3501771B196" modified="2017-09-27" version="1">MAKPSYVKFEVPQELAEKALEAVELARDTGRIRKGTNETTKAVERGQAKLVVIAEDVDPEEIVAHLPPLCEEKEIPYIYVPSKKELGAAAGLEVPSASVAIVEPGKARELVEDIATKVRELMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9I4</accession>
    <name>A0A218P9I4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_08995</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854690.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9I4"/>
    <dbReference type="GeneID" id="33316405"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08995"/>
    <dbReference type="OrthoDB" id="7513at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027552">
        <property type="entry name" value="CGP_CTERM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051918">
        <property type="entry name" value="STPP_CPPED1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04288">
        <property type="entry name" value="CGP_CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43143">
        <property type="entry name" value="METALLOPHOSPHOESTERASE, CALCINEURIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43143:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE CPPED1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="2">
        <location>
            <begin position="129"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="630"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="670" mass="73056" checksum="296CA46E6621E9C9" modified="2017-09-27" version="1">MQGKKLLALLMALLAVVAIPVSTVSAASASPAPGEILLQPLPGAPVIGSPGEIVEIRPAEGVSIQSLQIVSILHGPYDLEIIGTDNGVLKAKIPEDAVPDVYFLVVKSDEGEVAIPNGVWVVKENPKLLRIAHLSDIHVTSGAKIGFVCGDYFQKSLPEILKYCDHPYAMHSYTAHVSAMTYYAMTGLQGQGVVNVIVSTGDDVDTSGDQEGYRMFDHGILMGTAAGTPFISIKGNHDHPPTYYTKYVNPRYFYEVIGDFLIIGLDSRGEERHPEMEQLQWMEDVLKAHPNKTVIVLVHHPFWYSTPDGKWGGTIKGYTAFDDGDWSALTKFVSWDWEGRNGEYDDIARYFLQMVEKYNIRLVLAGHIHKDKPVLYVDKNGNEHWFYTVTTTGAPDKTSNPPSETDKKLGYTVPSWYGSQVIYVYSNGTVEFPLTGDVLHEGISSLPIPQKFIVYRQNGEDGTAIKFVNELDQTVSGPLVLQIPEGAKIDPDHTNITYTVLGEREIGGAYYVLINATVPEGISQIAAVKETDTKPPDVKVGYITPSKPKPGSLFKVYISASDNVGIRDMKVQIVSDGKVLAEYPAFSMKPSTVSATYLTTVPGVNATEFTIKVIATDFYDNTAEATYEVGGTTTTTTGSSQSTSQSSESTCGPGILVGLALLPVLLKRRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9P4</accession>
    <name>A0A218P9P4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Threonylcarbamoyl-AMP synthase</fullName>
            <shortName evidence="12">TC-AMP synthase</shortName>
            <ecNumber evidence="3 12">2.7.7.87</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">L-threonylcarbamoyladenylate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">A3L08_09390</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>L-threonine + hydrogencarbonate + ATP = L-threonylcarbamoyladenylate + diphosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:36407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="EC" id="2.7.7.87"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the SUA5 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.87" evidence="3 12"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854759.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9P4"/>
    <dbReference type="GeneID" id="33316484"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09390"/>
    <dbReference type="OrthoDB" id="39992at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061710">
        <property type="term" value="F:L-threonylcarbamoyladenylate synthase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11030:FF:000001">
        <property type="entry name" value="Threonylcarbamoyl-AMP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.870.10:FF:000008">
        <property type="entry name" value="Threonylcarbamoyl-AMP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.10">
        <property type="entry name" value="DHBP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11030">
        <property type="entry name" value="Threonylcarbamoyl-AMP synthase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="Sua5-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038385">
        <property type="entry name" value="Sua5/YwlC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005145">
        <property type="entry name" value="Sua5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010923">
        <property type="entry name" value="T(6)A37_SUA5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050156">
        <property type="entry name" value="TC-AMP_synthase_SUA5"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00057">
        <property type="entry name" value="L-threonylcarbamoyladenylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490">
        <property type="entry name" value="SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490:SF16">
        <property type="entry name" value="THREONYLCARBAMOYL-AMP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03481">
        <property type="entry name" value="Sua5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004930">
        <property type="entry name" value="Tln_factor_SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="YrdC-like" evidence="14">
        <location>
            <begin position="13"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR004930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR004930-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ASJ07515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="339" mass="37414" checksum="C2FD616B97E66A90" modified="2017-09-27" version="1">MTVVINMREGVDERKVKIAARFILEGKLVAFPTETVYGLGADALNEEAARRIFQAKGRPADNPLIVHIAEFEDLKRLAREIPDEARLLAERFWPGPLTMVLPRREEVPDVTTGGLDTVAVRMPAHPIALALIKASTPIAAPSANISGKPSPTLAEHVVDDFYGKIEAIIDGGETNIGVESTVIDLSSERPTLLRPGGLPLEEIEAVIGPVEIHPAVRGKLVDVARSPGMKYKHYSPNAQVIVVEGPRERVRQKIAELVKEYRSKGLRVGVMATEEYEADEFFHLGRTEEDVARNLFRALRELDKRGVDIIIAEGIEERGLGFAVMNRLRKAAGYRIVWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9R6</accession>
    <name>A0A218P9R6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">acetate--CoA ligase (ADP-forming)</fullName>
            <ecNumber evidence="2">6.2.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_09440</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP + CoA = acetyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15081"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="6.2.1.13" evidence="2"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854768.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9R6"/>
    <dbReference type="GeneID" id="33316494"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09440"/>
    <dbReference type="OrthoDB" id="18103at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043758">
        <property type="term" value="F:acetate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014089">
        <property type="entry name" value="AcCoA-synth-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043938">
        <property type="entry name" value="Ligase_CoA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032875">
        <property type="entry name" value="Succ_CoA_lig_flav_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02717">
        <property type="entry name" value="AcCoA-syn-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42793">
        <property type="entry name" value="COA BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42793:SF1">
        <property type="entry name" value="PEPTIDYL-LYSINE N-ACETYLTRANSFERASE PATZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19045">
        <property type="entry name" value="Ligase_CoA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13607">
        <property type="entry name" value="Succ_CoA_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA-binding" evidence="3">
        <location>
            <begin position="9"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="465" mass="50137" checksum="878C3798A0FBF091" modified="2017-09-27" version="1">MADSRIEALFKPKSVAVIGASGTPGKIGYAIMKNLVEYGYEGKIYAVNVKGGEIEISGRKFPVYKSILDVPDEVDMAVIVVPAKFVPQVVEECGQKGVKVLPIISSGFGELGEEGKKIEQQLVETAHKYGMRILGPNIFGVVYTPEKLNATFGPTDVMPGKLALISQSGALGIALMGWTILEKVGLSAVVSIGNKSDIDDADLLEYFEEDENTGAILIYMEGVKDGRKFMETAKKVSMKKPIIIIKAGRSERGAKAAASHTGSLAGADSIYTAAFKQSGVLRALTIGEAFDWARTLSNLPEPEGENVVILTNGGGIGVMATDAAEEEGLHLYDNLEELKVFANHMPPFGSYKNPVDLTGMAGAESYEGAIRDALAHPEMHSIAVLYCQTAVLDPRDLAKIVIREYNESGRKKPLVVAIVGGIEAKEAIDMLNEEGIPAYPEPERAIKSLAALYKWSNWKKKQKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Y7</accession>
    <name>A0A218P9Y7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09755</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854826.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Y7"/>
    <dbReference type="GeneID" id="33316557"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09755"/>
    <dbReference type="OrthoDB" id="86250at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="503" mass="58262" checksum="22F4A1C029514AD5" modified="2017-09-27" version="1">MEREILELLSLERTREPLSPGRRVREFQKTIQTLKNGEDVELKGFLLARKPPNAPRDAVYYLLSPLPPSELASLGENDFRTYLVIRATEETLVSGEVKPGNYVLVRGIIDAYPWGNMRVVYASSIEGMDYPDYWKDYQEFALSKSEVVDLFERTVYLRDDMRNALIYSVYGVPYIIGESWGEGFEFTVFKYRDDSGLLALWKAFKYFHSNLPWEVRLGSERVIEVDDPFLGIDFRLGNPNASDMRYYTPLTKRGLVKLPKKVAGDIVSKRAIGLLPRNLDADPLDRMARLSETPFVLVPSEEKPYFEENREFLQLIPNLLVTVFIQREKHKALDREKTRLLEEELLRWLKESRDDYGDPFRALTAPSGPMNVKLRAELGKRVFGSIVRFNGRVTKRAAREVKLINEAIVNDWMVVLKDRPREMMRLLREYRAYVPGTLKAQRALEILHDLASVSPSGEVTKEEFIRELVKDGFQREDALEITEKFIATGYVYEPFPGKIRPIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA12</accession>
    <name>A0A218PA12_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_09735</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854823.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA12"/>
    <dbReference type="GeneID" id="33316553"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09735"/>
    <dbReference type="OrthoDB" id="65749at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009639">
        <property type="entry name" value="Pept_A24A_C_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06819">
        <property type="entry name" value="Arc_PepC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="253"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A24A-predicted C-terminal archaea" evidence="2">
        <location>
            <begin position="257"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="401" mass="45108" checksum="BA1767EBE9FC9560" modified="2017-09-27" version="1">MGYAPLLLGLVMGAITSYTDLKTGFIDDINVFPTLALIGKLRGWEGEESEGLLDKIPIPAVEVGILYYLYLGLKEDNTLLAVSGLVGFVLGLILGLLLYYIGAWASGDVLILAGFSALLPYPPENASLVPPYAVGYPLYPLTILLNSLIAIFPFIFLYAFGVILLRRQFDELRRIFTDGARLTAEVSLWIMAALGFRLILYDFTGVAIVGIWSWLFTIVVIYVLGKFRKAGDVIGLAVLAYLLYTDPLPMARAFLKLLAMLYLFKVFFSLARFMRTGVLMEDVPVEELEEWDILGETVFEREGEVLRDRSDLFTRMKNAVTSADPSLLRPDYGRIIASPTAEGLRREQIEELRKLVEEGKLENRFLRKKSMPFAPALFLGFLISYFWGDIFWWIQVKIAGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA31</accession>
    <name>A0A218PA31_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pyridine nucleotide-disulfide oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_04955</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA31"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04955"/>
    <dbReference type="OrthoDB" id="27340at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051691">
        <property type="entry name" value="Metab_Enz_Cyan_OpOx_G3PDH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42949">
        <property type="entry name" value="ANAEROBIC GLYCEROL-3-PHOSPHATE DEHYDROGENASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42949:SF3">
        <property type="entry name" value="ANAEROBIC GLYCEROL-3-PHOSPHATE DEHYDROGENASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="2">
        <location>
            <begin position="4"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="413" mass="44920" checksum="E1A5541A17A2C01E" modified="2017-09-27" version="1">MLSYDVLVIGGGPAGMAAAVRARELGLKVLLLDENDYLGGILPQCIHPGFGLHYFREELTGPEFAARLAKKLIELGVEYRTSARVLEIKNYSDLEKVVIFTSPSGVYQVWTKAVIYAAGARERHAFEIGIVGDRVSGIYTAGEAQTLMDIYGILPGKEIVIVGSGDVGLIMARRFALEGAKVKAVVELMPYPGGLARNVMILKDFNIPLYLGHKVVEVRGKGRVQRVKVVKVDENLNEVPGSEFWIEADTLVISAGLVPAVKNLKKIGVEIDPATGGPVVNDRLETSVPGIFVAGNSLVINDLVDYVAEQGELAAEGAKEFIDNGGIESRKWVKVEKGSNVRLVVPHYLSGERDVYLYLRVAKPMEDVELGIPEIGKRLKLPVVKPAEMIRVKLKAEEIQNADGKLTVEVVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA36</accession>
    <name>A0A218PA36_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Digeranylgeranylglycerophospholipid reductase</fullName>
            <shortName evidence="8">DGGGPL reductase</shortName>
            <ecNumber evidence="8">1.3.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">2,3-bis-O-geranylgeranylglyceryl phosphate reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Geranylgeranyl reductase</fullName>
            <shortName evidence="8">GGR</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_04750</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Is involved in the reduction of 2,3-digeranylgeranylglycerophospholipids (unsaturated archaeols) into 2,3-diphytanylglycerophospholipids (saturated archaeols) in the biosynthesis of archaeal membrane lipids. Catalyzes the formation of archaetidic acid (2,3-di-O-phytanyl-sn-glyceryl phosphate) from 2,3-di-O-geranylgeranylglyceryl phosphate (DGGGP) via the hydrogenation of each double bond of the isoprenoid chains. Is also probably able to reduce double bonds of geranyl groups in CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol and archaetidylserine, thus acting at various stages in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2,3-bis-O-(phytanyl)-sn-glycerol 1-phosphate + 8 A = 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + 8 AH2</text>
            <dbReference type="Rhea" id="RHEA:64368"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:58837"/>
            <dbReference type="ChEBI" id="CHEBI:73125"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + 8 AH2 = CDP-2,3-bis-O-(phytanyl)-sn-glycerol + 8 A</text>
            <dbReference type="Rhea" id="RHEA:84207"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:58838"/>
            <dbReference type="ChEBI" id="CHEBI:74004"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 2,3-bis-O-phytanyl-sn-glycerol 1-phospholipid + 8 A = a 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phospholipid + 8 AH2</text>
            <dbReference type="Rhea" id="RHEA:64376"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:138139"/>
            <dbReference type="ChEBI" id="CHEBI:138140"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>archaetidylserine + 8 AH2 = 2,3-bis-O-phytanyl-sn-glycero-3-phospho-L-serine + 8 A</text>
            <dbReference type="Rhea" id="RHEA:84215"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:71517"/>
            <dbReference type="ChEBI" id="CHEBI:74853"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="8">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">Reduction reaction proceeds via syn addition of hydrogen for double bonds.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the geranylgeranyl reductase family. DGGGPL reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.3.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA36"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04750"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045550">
        <property type="term" value="F:geranylgeranyl reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046467">
        <property type="term" value="P:membrane lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01287">
        <property type="entry name" value="DGGGPL_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023590">
        <property type="entry name" value="DGGGPL_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011777">
        <property type="entry name" value="Geranylgeranyl_Rdtase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050407">
        <property type="entry name" value="Geranylgeranyl_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054715">
        <property type="entry name" value="GGR_cat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02032">
        <property type="entry name" value="GG-red-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685:SF18">
        <property type="entry name" value="DIGERANYLGERANYLGLYCEROPHOSPHOLIPID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685">
        <property type="entry name" value="GERANYLGERANYL DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12831">
        <property type="entry name" value="FAD_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22578">
        <property type="entry name" value="GGR_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0274">FAD</keyword>
    <keyword evidence="2 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="1 8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 8" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Digeranylgeranylglycerophospholipid reductase catalytic" evidence="9">
        <location>
            <begin position="174"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>a 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phospholipid</name>
            <dbReference type="ChEBI" id="CHEBI:138140"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>a 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phospholipid</name>
            <dbReference type="ChEBI" id="CHEBI:138140"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ07647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="393" mass="43857" checksum="7BC9926015FA5B5C" modified="2017-09-27" version="1">MKYDVVVVGAGIAGPIVARNVAKAGYSVLLIDKKPAIGTPKQCAEGISIKVFDKYDIPYDKRYINREIYGARLYSPSGYELELRYNEVSGVILERKVFDKMLAYYAAKAGADVLARTEARDVIRKDGKVAGIKAKHEDEPVEIYADIIVAADGVESMIARKAGINTYAPPHEFDSSYEYEMLIEGYDPDLIHLWFGNEVAPRGYVWVFPKDEDRANVGIGINSDNPNTAKYYLDKWLKENNIPMSRILEVNVGIVPVGGFVKELAKDNVVVVGDAARQVNPMHGGGMAEAMEAGTIASKWIVKALEEENLSLLQNYTKEWWETDGKRLERVLKVRKVTEKLTDEDLDLFIQLLSGADAEKIAGGDYVEVIKALLKHPKVLMSKRRLSLLKQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA55</accession>
    <name>A0A218PA55_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphatidylethanolamine-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854901.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA55"/>
    <dbReference type="GeneID" id="33315825"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06110"/>
    <dbReference type="OrthoDB" id="28720at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00865">
        <property type="entry name" value="PEBP_bact_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005247">
        <property type="entry name" value="YbhB_YbcL/LppC-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00481">
        <property type="entry name" value="YbhB/YbcL family Raf kinase inhibitor-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30289:SF1">
        <property type="entry name" value="PEBP (PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30289">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YBCL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="155" mass="17124" checksum="DEB3D5AE6E92BE6D" modified="2017-09-27" version="1">MDLEIGSVFHDGDYIPVEFTCDGEDVNPPIFIGHIDPNVKSLVIIMDDPDAPMGTFTHWIAWNIPPLGEIPKGVPPKGVVEAPVRMVQGRNDFGKIGYGGPCPPKGHGVHHYHFKIYALDTVLELDPGTSRKELEKAMEGHIIQMGELVGLYERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA84</accession>
    <name>A0A218PA84_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SHOCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_08030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA84"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08030"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018649">
        <property type="entry name" value="SHOCT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09851">
        <property type="entry name" value="SHOCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="SHOCT" evidence="2">
        <location>
            <begin position="48"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="76" mass="9074" checksum="F708817BF22B3A62" modified="2017-09-27" version="1">MMGFGWFGWFGMLFMVIFWVAVIVGIAWLVKWLSEQGSGGNRSSRRRALEILDEKYARGEIDDKEYERRRRKLLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P4Y6</accession>
    <name>A0A218P4Y6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_00115</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853111.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P4Y6"/>
    <dbReference type="GeneID" id="33314627"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00115"/>
    <dbReference type="OrthoDB" id="117324at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05126">
        <property type="entry name" value="Mth938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1230.10">
        <property type="entry name" value="MTH938-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034096">
        <property type="entry name" value="AAMDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036748">
        <property type="entry name" value="MTH938-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007523">
        <property type="entry name" value="NDUFAF3/AAMDC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15811">
        <property type="entry name" value="MTH938 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15811:SF5">
        <property type="entry name" value="MTH938 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04430">
        <property type="entry name" value="DUF498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64076">
        <property type="entry name" value="MTH938-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ05848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="118" mass="13413" checksum="6795081554ABBCE0" modified="2017-09-27" version="1">MKAKYPGFGKIIIDGKVYDHDVVVYPSGKIEKRKKWLSKDKHGTSHKLDPDELREYLKEDFDVLLIGTGAYGMLSLLPESRELVKGKEVIELPTGKAVERLNEMAKKKRVLAVFHVTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P507</accession>
    <name>A0A218P507_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MFS transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853128.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P507"/>
    <dbReference type="GeneID" id="33314644"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00200"/>
    <dbReference type="OrthoDB" id="85336at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="358"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="401" mass="44124" checksum="879AD5892BB1D70A" modified="2017-09-27" version="1">MDRKWLTVIMNTLVVASGFGTMHMLEKFKDVVIAHYGITEAMMSYQQTAYVVGLFVAFLLGGTSLFKGSFKRSVALIVSFAAIPQFLIPFMPNWWGIVALRFFQGFIVALVAVFSNQIARLFLAERPFAKGIVLSGIFWGGIYGINLAKWASHSYAGWKAVEVAFLLSAIVMFIMLALWWFFVEDFEIPKEKHSAKGANVWKMPFTWVFGFTFFPALWIVFTLGSFTLHNVDFSDAQLANLVMTLEVSMGVWSIIMGYLGYRLSLKNTSNRGLFKAIVSVMTTSYALTFLGLFILWKGISANDYTLALLGLAITGIVQGTGPAFWTTAPAAYPKEIYPEASFALGLISNSANAVAPNVMFVLVHGVSTGVTIYLGMALLGIVLLLASSRMKLPVEELGDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P530</accession>
    <name>A0A218P530_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Oligopeptide ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_00280</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853142.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P530"/>
    <dbReference type="GeneID" id="33314660"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00280"/>
    <dbReference type="OrthoDB" id="18209at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000016">
        <property type="entry name" value="Oligopeptide ABC transporter ATP-binding component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050319">
        <property type="entry name" value="ABC_transp_ATP-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43776:SF7">
        <property type="entry name" value="D,D-DIPEPTIDE TRANSPORT ATP-BINDING PROTEIN DDPF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43776">
        <property type="entry name" value="TRANSPORT ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="6"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ05879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="326" mass="37119" checksum="EE7B5A9F46124EB4" modified="2017-09-27" version="1">MSEPILEVKNLKKYFPIRGLIRTEGYVKAVDDISFTINRGETFGLVGESGCGKTTTGRTILRLIEPTAGQIIFQGKDVTQLKGEEMKWFRRKAQIMFQDPYSSLNPRQTVFEIIMEPVRFHKIPVDDPEEFVIKLLESVGLNEMHLYRYPHEFSGGQRQRIALARLLALKPEFIVLDEPTSALDVSVQANILNTLKDLQKEFGFTYLFISHDLGVVKYMSHRMGVMYLGKLVEVGPAEEIFENPLHPYTKFLLSAIPVPDPELAKELKAKRMKVEGEPPSPINPPQGCRFHPRCPFAKAGLCDRKEPPLIEVENGHYVACWLYGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P533</accession>
    <name>A0A218P533_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_00350</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853156.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P533"/>
    <dbReference type="GeneID" id="33314674"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00350"/>
    <dbReference type="OrthoDB" id="132546at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050194">
        <property type="entry name" value="Glycosyltransferase_grp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947:SF3">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="2">
        <location>
            <begin position="14"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="1">
        <location>
            <begin position="189"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ05893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="385" mass="42087" checksum="B761FB7421769B19" modified="2017-09-27" version="1">MRIALVSDWYYPKVGGVASHMHHLAAHLRKRGHEVAVVTNDLKTGKEEELEELGIELIKIPGTVSPIISINITYGLKSNLELGEFLRDFEVVHAHHAFTPLSLKAVKAGRNLGKATLLTTHSISFSHESSLWRALGLTFPLFSHYLSFPHGIIAVSNAAKAFIEHFTSVPVRVIPNGVDDEVFRPLSQKEKKRVKEELGIEGRVVLYVSRMSFRKGPHVLLNAFQNLSREFEDATLVMVGSGEMLPFLKAQAKFLGIEDRVVFTGYVPGNTLPKLFGMADVFVLPSTTAEAFGIVILEAMASGIPVVASNVGGIPEVVKESGSGLLVPPSDEVALKEAVQTILDDEKLAGDLGRAGRKAVERKYSWKVVASEIESAYNEVLSTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P551</accession>
    <name>A0A218P551_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dihydropyrimidine dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_00335</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853153.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P551"/>
    <dbReference type="GeneID" id="33314671"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00335"/>
    <dbReference type="OrthoDB" id="27922at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028261">
        <property type="entry name" value="DPD_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006004">
        <property type="entry name" value="SudA-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01316">
        <property type="entry name" value="gltA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42783">
        <property type="entry name" value="GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42783:SF3">
        <property type="entry name" value="GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14691">
        <property type="entry name" value="Fer4_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydroprymidine dehydrogenase" evidence="2">
        <location>
            <begin position="22"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="159"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ05890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="481" mass="52897" checksum="EAF35020DFC69940" modified="2017-09-27" version="1">MAVRRKLIKERVPTPERPAEERVKDFAEVNLGYTFELAVKEAERCLQCPYDYAPCIKGCPVHINIPGFISKLVEYRDDPDKAVKEALNVIWACNSLPATTGRVCPQEDQCEMNCVMGKVGDKINIGKLERFVADYAREKGIDEELLFEIIPKIEKKGQSVAIIGAGPAGLTAAGELAKLGYDVTIYEALHEAGGVLMYGIPEFRLPKSIVESEIEKLKKLGVKILTDHVVGRTVTIEELLEEYDAVFIGSGAGTPRLVNAPGVNLNGIYTANEFLTRVNLMKAYKFPEYDTPVYVGKKVIVIGAGNTAMDAARSARRFGADVTITYRRGPEDVSARIEEVHHAKEEGIKFEYFINPVEFIGDENGKVKAVKFEKMKALEEKDSRGKRKIVGTGEYVTLEADTVIIAIGKHPNRLIVNTPGLKVERGKIVVDENLMTSIPGVFAGGDAIRGEATVILAMGDGRKAAKAIHEYLTKKREGQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P563</accession>
    <name>A0A218P563_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_00435</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853171.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P563"/>
    <dbReference type="GeneID" id="33314691"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00435"/>
    <dbReference type="OrthoDB" id="86190at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ05908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="270" mass="31454" checksum="3D2893232E66917A" modified="2017-09-27" version="1">MRLEVLSKEDMNALSRELSRAGIMNRTKEEVSPQISHYIVIEETYAELLEKAEGIEPLEEYLEELRAAYEEMLADWNVGEERTLEGLFDESDPGKLILVTALMESEAVKEDNGKLILKEKPALDVLKVELRFPIEELEEYLEELEERFNPSMVTEYILEKNYYVEVMEVDRELVEAALEIAEEYATEESLVEAMFDGIARSVLADVILELAEKHRRKNELMEVLMEREPIVIEGERERVNIYFDEEAVEDLLKELQTLGYLKVKGNRIWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P571</accession>
    <name>A0A218P571_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7343 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_00445</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853173.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P571"/>
    <dbReference type="GeneID" id="33314693"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00445"/>
    <dbReference type="OrthoDB" id="85590at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051922">
        <property type="entry name" value="Bact_Sporulation_Assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007253">
        <property type="entry name" value="Cell_wall-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055767">
        <property type="entry name" value="DUF7343"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30032:SF4">
        <property type="entry name" value="AMIDASE ENHANCER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30032">
        <property type="entry name" value="N-ACETYLMURAMOYL-L-ALANINE AMIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04122">
        <property type="entry name" value="CW_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24034">
        <property type="entry name" value="DUF7343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7343" evidence="2">
        <location>
            <begin position="266"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ05910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="334" mass="37273" checksum="5232995452BEAA78" modified="2017-09-27" version="1">MVKRAVALTLVVVVLSSFLLPLSSAQEKVWEPQYDLIIVRDDDLIDYIIAIPYAKMLGVPILPVNPTELDPSTIAQLQSYAQFGWNHVLIIGDSQAVSDKVQDELLNIGFVVERIGGAVRTETAAKLALHFYPSGHDTVVLASSSDYGSALAAARWASIRDLPMLLTQEDALSDSTVHALEELHPHLVILMGAGMSKDIQKKLEEMGYQTYWFKENLKIEVPEQPKETNWMTVILAVVASLAVAVPVSFYYAKKKWAANKVPIEVLTEKERIVVKAILEKGGTVKQEDLPELTGYSRPTISRIIQELEKKQLVEREKIGKTFIVKLTKEIIIRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P599</accession>
    <name>A0A218P599_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable RNA 2'-phosphotransferase</fullName>
            <ecNumber evidence="5">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">kptA</name>
        <name evidence="6" type="ORF">A3L08_00650</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Removes the 2'-phosphate from RNA via an intermediate in which the phosphate is ADP-ribosylated by NAD followed by a presumed transesterification to release the RNA and generate ADP-ribose 1''-2''-cyclic phosphate (APPR&gt;P). May function as an ADP-ribosylase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the KptA/TPT1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853211.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P599"/>
    <dbReference type="GeneID" id="33314734"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00650"/>
    <dbReference type="OrthoDB" id="24376at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000215">
        <property type="term" value="F:tRNA 2'-phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.170.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.970">
        <property type="entry name" value="RNA 2'-phosphotransferase, Tpt1/KptA family, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00299">
        <property type="entry name" value="KptA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002745">
        <property type="entry name" value="Ptrans_KptA/Tpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042081">
        <property type="entry name" value="RNA_2'-PTrans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022928">
        <property type="entry name" value="RNA_2'-PTrans_KptA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042080">
        <property type="entry name" value="RNA_2'-PTrans_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002013">
        <property type="entry name" value="PRK00819.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12684">
        <property type="entry name" value="PUTATIVE PHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12684:SF2">
        <property type="entry name" value="TRNA 2'-PHOSPHOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01885">
        <property type="entry name" value="PTS_2-RNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ05949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="181" mass="20776" checksum="40772EF423278728" modified="2017-09-27" version="1">MKWSTRTRVSKLMAYILRHSPEEFGLKPDVEGFVPLGELLKALNTVYPDVDEEFVRNIVANDPKGRYEIKGDRIRARYGHSFPVSLNHEEDTESRFLYHGTPRRNLPSILREGLKPMKRQFVHLSTSKSEALETGRRHGRDVVLLITDADCLRRNGLRIFKAGKNVRIVKGVPPDCIILEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5A9</accession>
    <name>A0A218P5A9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">nicotinate phosphoribosyltransferase</fullName>
            <ecNumber evidence="2">6.3.4.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_00740</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>5-phospho-alpha-D-ribose 1-diphosphate + nicotinate + ATP + H2O = nicotinate beta-D-ribonucleotide + ADP + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36163"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32544"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.21" evidence="2"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853224.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5A9"/>
    <dbReference type="GeneID" id="33314752"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00740"/>
    <dbReference type="OrthoDB" id="371831at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00457"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004516">
        <property type="term" value="F:nicotinate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01571">
        <property type="entry name" value="NAPRTase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.140.10">
        <property type="entry name" value="nicotinate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.20">
        <property type="entry name" value="Quinolinate phosphoribosyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053190">
        <property type="entry name" value="NAPRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035809">
        <property type="entry name" value="NAPRTase_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041619">
        <property type="entry name" value="NAPRTase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007229">
        <property type="entry name" value="Nic_PRibTrfase-Fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006415">
        <property type="entry name" value="PRK08662.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43202:SF1">
        <property type="entry name" value="NICOTINATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43202">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17956">
        <property type="entry name" value="NAPRTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000484">
        <property type="entry name" value="NAPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54675">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase N-terminal" evidence="9">
        <location>
            <begin position="19"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase C-terminal" evidence="8">
        <location>
            <begin position="115"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Nicotinate phosphoribosyltransferase C-terminal" evidence="10">
        <location>
            <begin position="343"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ05962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="390" mass="43450" checksum="7BFBD35A914DA8EC" modified="2017-09-27" version="1">MREFYIAHEDDIRAGKTTDVYFIRTKKILEEKGIHKKVFADVSTTSLPNGWKWGVLAGIEEVAKLLEGLPVNVYAMPEGTIFHPYEPVLQIEGYYEEFGIYETALLGMLSQASGIATAALRVKIAANFKPVYSFGIRHMHPAIAPMIDRSAFIGGCDGVSGVLGAEMIGEKPVGTMPHALIITVGDQVKAWKYYDEVMPPEVPRTALVDTFYDEKFEALMAAEALGERLNAVRLDTPGSRRGNFRRIIEEVRWELDLRGYDWVKIFVSGGLNEDSIREIVDVADAFGVGGSIASAKPIDFSLDIVEVEGRPITKRGKLSGRKQIYRCENGHYHRVPADKKLEHCPVCGAKVEPLLKPLIENGEIVAELPKAREIREYVLEQAKKFNLALD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5F6</accession>
    <name>A0A218P5F6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Threonine synthase</fullName>
            <ecNumber evidence="5">4.2.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_01020</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the threonine synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.1" evidence="5"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853274.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5F6"/>
    <dbReference type="GeneID" id="33314808"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01020"/>
    <dbReference type="OrthoDB" id="6371at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004795">
        <property type="term" value="F:threonine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01563">
        <property type="entry name" value="Thr-synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004450">
        <property type="entry name" value="Thr_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00260">
        <property type="entry name" value="thrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF6">
        <property type="entry name" value="L-THREONINE DEHYDRATASE CATABOLIC TDCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="7">
        <location>
            <begin position="57"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="NCBIfam" id="TIGR00260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604450-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="383" mass="41926" checksum="74D1D774083AD589" modified="2017-09-27" version="1">MKLKCPVCGREYEEPVQRCECGEPVEFELLKGEPYIGKSVWERFWDFWPVEPELELSLGEGDTPLIRSKLGKELDVKLYLKNETVNPTWSFKDRGTFLAMSYALKAGYKAVGTVSTGNMAASVAAYASRFGLDAKILVSESASDEKLKAVSVYGGDVIRVKGDYGRLYFESLKLGERLGVYFINSDNPFRIEGYKGITFEIAEELSPDYVLVPTSSGGLFRGIAKGFIELKESGLIDDLPTLVAVQAEGCSPICRAFKEGRERIERFEKPKTIAKAIANPYPPSGNAVLKLLREFGWLCVSVNDEEIATAQRKLAGEGFFVQPASATGVAALEKLTESGVISEGSKVVSILTGSGLKTLFQVEGDEIRECPLEGLESCIRSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5H8</accession>
    <name>A0A218P5H8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secondary thiamine-phosphate synthase enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0047 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853282.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5H8"/>
    <dbReference type="GeneID" id="33314817"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01065"/>
    <dbReference type="OrthoDB" id="6663at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.460">
        <property type="entry name" value="YjbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001602">
        <property type="entry name" value="UPF0047_YjbQ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035917">
        <property type="entry name" value="YjbQ-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00149">
        <property type="entry name" value="TIGR00149_YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YJBQ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615:SF8">
        <property type="entry name" value="UPF0047 PROTEIN C4A8.02C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01894">
        <property type="entry name" value="YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004681">
        <property type="entry name" value="UCP004681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111038">
        <property type="entry name" value="YjbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="137" mass="15405" checksum="6B94E703153B3B3E" modified="2017-09-27" version="1">MKVHVEELRFSTEGEIDLVDITHEVEGVVKESGIKNGQVLVFVPGATGAIVTIEHESGLLEDFKRALRELVPKGKGYLHDRIDDNAHSHLRATLLGASECFPVIDGRLVRGTWQQIFFVELDVRPRHRRVIVQVMGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5I1</accession>
    <name>A0A218P5I1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF523 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853290.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5I1"/>
    <dbReference type="GeneID" id="33314826"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01110"/>
    <dbReference type="OrthoDB" id="240616at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="198" mass="22878" checksum="F0C5E03648444AED" modified="2017-09-27" version="1">MNILVIAPCLLSPFYVYRGPKEKEYETAKRLRELLGKLDDEWQVLVYPCPEFELIGWPRAPASREVYEKLGMRERAKVIADFVGRVLTEEKPEKVVFVGVKGSPTCGVFHTSSSDPEKYPYMAMQEFFYLGKEERLKRSKELVGEQGFRLVEIPGILFEILMARFPEGVYVEFDKGDIEESLNRLANILEHHGSQQVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5L1</accession>
    <name>A0A218P5L1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Fumarate hydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_01240</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I fumarase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853312.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5L1"/>
    <dbReference type="GeneID" id="33314852"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01240"/>
    <dbReference type="OrthoDB" id="371925at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051208">
        <property type="entry name" value="Class-I_Fumarase/Tartrate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004646">
        <property type="entry name" value="Fe-S_hydro-lyase_TtdA-typ_cat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004885">
        <property type="entry name" value="PRK06246.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00722">
        <property type="entry name" value="ttdA_fumA_fumB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30389">
        <property type="entry name" value="FUMARATE HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30389:SF17">
        <property type="entry name" value="L(+)-TARTRATE DEHYDRATASE SUBUNIT ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05681">
        <property type="entry name" value="Fumerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe-S hydro-lyase tartrate dehydratase alpha-type catalytic" evidence="7">
        <location>
            <begin position="6"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="286" mass="31173" checksum="657FFEDFC52896BD" modified="2017-09-27" version="1">MSLLVEALVEAIRLAVTRIPDDTVEALKRAYEEENSRIARFNLENILRAIEIGRERSIPVCQDTGTMTFFIRAGIESPFLKELEGAIIEAVRKATLEVPLRPNAVDVLGGRNSGDNTGKGVPIIHWELDKGDEVEIAVFPKGGGSENCSALAMLTPGEGWEGVRRFVVERVRACGGKPCPPVIIGIGVGGGADYSLKLAKRALLRKIGERNPDKEIAKIEEELLEEINALGIGPMGMGGKTTALDVKIEVAHRHPASFPVGLVVQCWANRRAFLRLKPNGRVEVWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Q4</accession>
    <name>A0A218P5Q4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Glyceraldehyde-3-phosphate:ferredoxin oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_01460</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>tungstopterin</name>
            <dbReference type="ChEBI" id="CHEBI:30402"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AOR/FOR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853353.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5Q4"/>
    <dbReference type="GeneID" id="33314897"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01460"/>
    <dbReference type="OrthoDB" id="84495at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.569.10">
        <property type="entry name" value="Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.9.10">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013984">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013983">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036503">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053617">
        <property type="entry name" value="GAPOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001203">
        <property type="entry name" value="OxRdtase_Ald_Fedxn_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036021">
        <property type="entry name" value="Tungsten_al_ferr_oxy-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051919">
        <property type="entry name" value="W-dependent_AOR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040818">
        <property type="entry name" value="GAPOR_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30038">
        <property type="entry name" value="ALDEHYDE FERREDOXIN OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30038:SF7">
        <property type="entry name" value="TUNGSTEN-CONTAINING GLYCERALDEHYDE-3-PHOSPHATE:FERREDOXIN OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01314">
        <property type="entry name" value="AFOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02730">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00790">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48310">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, C-terminal domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56228">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0826">Tungsten</keyword>
    <feature type="domain" description="Aldehyde ferredoxin oxidoreductase N-terminal" evidence="10">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="652" mass="74114" checksum="8B74E4F1FA964FA0" modified="2017-09-27" version="1">MRFTVLHLKLDEKKIESENFEKDGVYGVIDYGLELHEKLETHSVDPYDPKNVVVMGMGPFSGSILPGAHRLMFFFRSPLYGTLFPSAMGGAAYAFKNVGVDFVTFEGKAEKPVVVILYNDGENVKVELHEIELERLIEIWRGYKGEEGVYALTQYLIDTFGERFDFEYRIAVVGPAALNSNYGAIFSQTLRKGKRVEGSEDWAARGGSGSVLLRAHNVVGIIFGGKPRKRAFPGEDISSFRTAKEIVEGVHKKPYNDVIAEKTVKYKYNPKLKTGGTFGGNYPAEGDFVPILNWQMPYIEKEERIKIHEAIMKHYWEPFNKEAIEPKNWTNCGEPCPVVCKKYANGHHIEYEPREANGPLSGVITLRASDISVPAVDAMGFDAIEFGGTAAWVLELVHRGLLKPEEVGLSDKPDFTKDALLERPVEASEKNAKLVAELAHRVAFAENEIAKIIGLGKRKASVILDERFKDRLKYGESFKDYAVFTPLGEDGEMTPTMYWAIGNYIPLPVQGRYWTFYQFGVFLEPEELAQKIIASALWEFWYDNVGWCRFHRGWMKPVLKALFMDAYGENVDMEEHARKQIKRLIEFARKAGYTPVFWDSMRVIDLVARGSEEFGNERWAEKFKIDKVGTAKEYLKRILDAYSEALGVEWRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Q7</accession>
    <name>A0A218P5Q7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable aminomethyltransferase</fullName>
            <ecNumber evidence="5">2.1.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Glycine cleavage system T protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">gcvT</name>
        <name evidence="9" type="ORF">A3L08_01410</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">The glycine cleavage system catalyzes the degradation of glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[protein] + (6S)-5,6,7,8-tetrahydrofolate = N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16945"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:10492"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="2.1.2.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GcvT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.10" evidence="5"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853344.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5Q7"/>
    <dbReference type="GeneID" id="33314887"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01410"/>
    <dbReference type="OrthoDB" id="2001at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004047">
        <property type="term" value="F:aminomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.110:FF:000003">
        <property type="entry name" value="Aminomethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.110">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1400">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1250.10">
        <property type="entry name" value="Aminomethyltransferase fragment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00259">
        <property type="entry name" value="GcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006223">
        <property type="entry name" value="GcvT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028896">
        <property type="entry name" value="GcvT/YgfZ/DmdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029043">
        <property type="entry name" value="GcvT/YgfZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022903">
        <property type="entry name" value="GcvT_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013977">
        <property type="entry name" value="GcvT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006222">
        <property type="entry name" value="GCVT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00528">
        <property type="entry name" value="gcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001567">
        <property type="entry name" value="PRK00389.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757">
        <property type="entry name" value="AMINOMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757:SF2">
        <property type="entry name" value="AMINOMETHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01571">
        <property type="entry name" value="GCV_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08669">
        <property type="entry name" value="GCV_T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006487">
        <property type="entry name" value="GcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101790">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103025">
        <property type="entry name" value="Folate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GCVT N-terminal" evidence="7">
        <location>
            <begin position="8"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Aminomethyltransferase C-terminal" evidence="8">
        <location>
            <begin position="311"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006487-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="398" mass="45192" checksum="B612866E64166680" modified="2017-09-27" version="1">MVKRVHIFDWHKEHAKKVEEFAGWEMPIWYSSIKDEHLAVRNGVGIFDVSHMGEFIFRGKDALEFLQYVTTNDISKPPAISGTYTLVLNERGAVKDETLVFNMGNDTYMMVCDSDAFEKLYAWFNAIKRGIEKFGELDLEIENKTYDMAMFSIQGPKARDLAKDLFGIDINELWWFQAKEVELDGIKMLLSRSGYTGENGWEVYFEDANPYHPDPSKRGEPEKALHVWKTILEAGEQYGIKPAGLGARDTLRLEAGYTLYGNETKEKQLLSTDIDEVTPLQANLDFALFWDKEFIGKDALLKQRERGLPSKMVHFKMVDKGIPREGYKVYANGEPIGEVTSGTLSPLLGIGIGIAFVKPEYAKPGVEIEVEIRGNPKKAVTVAPPFYDPKKYGAFREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5W5</accession>
    <name>A0A218P5W5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rubrerythrin diiron-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5W5"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01805"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01045">
        <property type="entry name" value="Ferritin_like_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rr_diiron-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33531:SF10">
        <property type="entry name" value="BLR7895 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33531">
        <property type="entry name" value="RUBRERYTHRIN SUBFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rubrerythrin diiron-binding" evidence="1">
        <location>
            <begin position="24"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="179" mass="20822" checksum="ADC9EE3B9BB7472F" modified="2017-09-27" version="1">MENLEDTLKKFRDMLLSLSEKGILSYWTFGEYEEAKIYWELAKKAEELRLPPSLVSTFRVLAKESEEHGDGLRRLYVKTYGEEPKKVDLPYIEAVSLARALEDPSNIDLVFRVAMETELIAKKLYEHLASITENEEAKGLYEFLANVEWTHYQRLRGEAELMGINVGKIEEEIRKKGFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5W7</accession>
    <name>A0A218P5W7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_01895</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853430.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5W7"/>
    <dbReference type="GeneID" id="33314984"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01895"/>
    <dbReference type="OrthoDB" id="6762at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154">
        <property type="entry name" value="LEUCINE-RESPONSIVE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR AZLB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="5"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="150" mass="16905" checksum="5105BA6660917F38" modified="2017-09-27" version="1">MVNALDAADMRLLKELKENSRENIASLSKKLGIPRTTVHYRIKKLVEDGVIEKFTVKPNYKKLNLGTTAFILARYDPDSGLNQREVAERIAALEGVYEVHIIAGEWDLLIKVRAPSSEEVGRIVVDRLREIRGVGQTVTMVSFVTVKEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5X7</accession>
    <name>A0A218P5X7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">S-methyl-5'-thioadenosine phosphorylase</fullName>
            <ecNumber evidence="5">2.4.2.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">5'-methylthioadenosine phosphorylase</fullName>
            <shortName evidence="5">MTA phosphorylase</shortName>
            <shortName evidence="5">MTAP</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mtnP</name>
        <name evidence="7" type="ORF">A3L08_02055</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>S-methyl-5'-thioadenosine + phosphate = 5-(methylsulfanyl)-alpha-D-ribose 1-phosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:11852"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="EC" id="2.4.2.28"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 5">Homohexamer. Dimer of a homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.28" evidence="5"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853460.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5X7"/>
    <dbReference type="GeneID" id="33315016"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02055"/>
    <dbReference type="OrthoDB" id="7681at2157"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00873"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017061">
        <property type="term" value="F:S-methyl-5-thioadenosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09010">
        <property type="entry name" value="MTAP_SsMTAPII_like_MTIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1580:FF:000012">
        <property type="entry name" value="Probable 6-oxopurine nucleoside phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01963">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010044">
        <property type="entry name" value="MTAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018099">
        <property type="entry name" value="Purine_phosphorylase-2_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01694">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006334">
        <property type="entry name" value="PRK08564.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679">
        <property type="entry name" value="S-METHYL-5'-THIOADENOSINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679:SF3">
        <property type="entry name" value="S-METHYL-5'-THIOADENOSINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01240">
        <property type="entry name" value="PNP_MTAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3 5" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="6">
        <location>
            <begin position="3"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="50"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="83"/>
            <end position="84"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="204"/>
            <end position="206"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="5">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="5">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00063054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="257" mass="29043" checksum="83301D59A5215D0D" modified="2017-09-27" version="1">MVKIGIIGGSGVYGVFEPKETVKVHTPYGRPSAPVEIGEIEGVEVAFIPRHGKNHEFPPHEVPYRANIWALHELGVERVIGVTAVGSLREEYKPGDIVITDQFIDFTKKRDYTFYNGPRVAHVSMADPFCPEMRKIFYETAKELGFPVHEKGTYVCIEGPRFSTRAESFMFRQYAHIIGMTLVPEINLARELGMCYANIATVTDYDVWADKPVDAQEVLKVMAENNYKVQELLKKAIPRIPEERKCGCADVLKSMFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Z8</accession>
    <name>A0A218P5Z8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">DNA methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_02145</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853476.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5Z8"/>
    <dbReference type="GeneID" id="33315034"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02145"/>
    <dbReference type="OrthoDB" id="7080at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016423">
        <property type="term" value="F:tRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11715">
        <property type="entry name" value="THUMP_AdoMetMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2130.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RlmKL-like_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053943">
        <property type="entry name" value="RlmKL-like_Mtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053796">
        <property type="entry name" value="Trm14-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040850">
        <property type="entry name" value="Trm14_Thcoccales"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14911">
        <property type="entry name" value="THUMP DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14911:SF13">
        <property type="entry name" value="TRNA (GUANINE(6)-N2)-METHYLTRANSFERASE THUMP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01261">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="THUMP" evidence="8">
        <location>
            <begin position="69"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="365" mass="40923" checksum="B2FE2D27C58F7821" modified="2017-09-27" version="1">MRLLLTTSQGIEDLAKAEVENLLSELGVPFRVEEKPLGVEGRVLAEVGEAFYTDEKGRKRELSVGTYLNERSRLLHRVIVEMASDRFEGIGEDEPEKALGRIEDFVASLPVERFVKVSESFAVRSFRKGEHKITSLDISRTVGKAIFDRLSRFGTPRVNLDHPAVIFRTELVGEVFFLGIDTTGDSSLHKRPWRVYDHPAHLKASIANALIELAEPDGGSFIDPFCGSGTIPIELALRGYSGRVICLEKYRKHLRGAEMNALSAGVYDKIEFILGDATKLSEYVDRVDFAVSNLPYGLKIGRKSMIPKLYMDFFGELAKVLEKRGVFITTEKKAIEKAIEENGFEIKHHRLIGHGGLMVHTYVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P619</accession>
    <name>A0A218P619_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pyrrolidone-carboxylate peptidase</fullName>
            <ecNumber evidence="9">3.4.19.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">5-oxoprolyl-peptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Pyroglutamyl-peptidase I</fullName>
            <shortName evidence="9">PGP-I</shortName>
            <shortName evidence="9">Pyrase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pcp</name>
        <name evidence="12" type="ORF">A3L08_02250</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9">Removes 5-oxoproline from various penultimate amino acid residues except L-proline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9 10">
            <text>Release of an N-terminal pyroglutamyl group from a polypeptide, the second amino acid generally not being Pro.</text>
            <dbReference type="EC" id="3.4.19.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the peptidase C15 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.3" evidence="9"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853495.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P619"/>
    <dbReference type="GeneID" id="33315055"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02250"/>
    <dbReference type="OrthoDB" id="39672at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016920">
        <property type="term" value="F:pyroglutamyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00501">
        <property type="entry name" value="Peptidase_C15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.20:FF:000001">
        <property type="entry name" value="Pyrrolidone-carboxylate peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.20">
        <property type="entry name" value="Peptidase C15, pyroglutamyl peptidase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00417">
        <property type="entry name" value="Pyrrolid_peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000816">
        <property type="entry name" value="Peptidase_C15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016125">
        <property type="entry name" value="Peptidase_C15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036440">
        <property type="entry name" value="Peptidase_C15-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029762">
        <property type="entry name" value="PGP-I_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033694">
        <property type="entry name" value="PGPEP1_Cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033693">
        <property type="entry name" value="PGPEP1_Glu_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009676">
        <property type="entry name" value="PRK13197.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00504">
        <property type="entry name" value="pyro_pdase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402">
        <property type="entry name" value="PROTEASE FAMILY C15 PYROGLUTAMYL-PEPTIDASE I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402:SF1">
        <property type="entry name" value="PYROGLUTAMYL-PEPTIDASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01470">
        <property type="entry name" value="Peptidase_C15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015592">
        <property type="entry name" value="Prld-crbxl_pptds"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00706">
        <property type="entry name" value="PYROGLUPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53182">
        <property type="entry name" value="Pyrrolidone carboxyl peptidase (pyroglutamate aminopeptidase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01334">
        <property type="entry name" value="PYRASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01333">
        <property type="entry name" value="PYRASE_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0788">Thiol protease</keyword>
    <feature type="active site" evidence="9 10">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <feature type="active site" evidence="9 11">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="202" mass="21915" checksum="219550609851059D" modified="2017-09-27" version="1">MKVLVTGFEPFGGESINPSWEAVKALPDEINGAELVKHRLPVTFGGVREILPRLIVKERPDVVVLTGQAGGRPNITVERVAINVMDSKMPDNEGFAPEDEPVFEGAPAAYFATIPIKAIVKALREEKIPASVSNTAGTYVCNTAMFTALHTIAVAGMETRAGFIHVPFSHEQALDKPRPSMAQETINRAIKRAVEVSIEWEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P644</accession>
    <name>A0A218P644_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_02335</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853510.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P644"/>
    <dbReference type="GeneID" id="33315072"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02335"/>
    <dbReference type="OrthoDB" id="28446at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051598">
        <property type="entry name" value="TSUP/Inactive_protease-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43701">
        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN MJ0441-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43701:SF2">
        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN YJNA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="197"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="226"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="245" mass="25925" checksum="FCCE582A50C8FA4E" modified="2017-09-27" version="1">MNPVFVGLGLLVGFLVGLTGVGGGALMTPSLIFLGVEPLVAVGTDLLYATVTRVFGVFFHGRKGRIRYDIALRLFAGSAPAIILGGVLLRWVNREVLNEYLTVLLGAVLVASAVLSLLKGEIHLPIRPRWAYVYLLGFVVGLTVQFTSVGAGVIVSFTLMNVVRLEPKDVVGVTITYGLALSTFSFMNYASVGSVDYGIASLLVLGTIPGVYLGTHVSRNADGAKLKKAINIIILLIGLFTLMSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P679</accession>
    <name>A0A218P679_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853536.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P679"/>
    <dbReference type="GeneID" id="33315101"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02480"/>
    <dbReference type="OrthoDB" id="86287at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43471">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43471:SF12">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12679">
        <property type="entry name" value="ABC2_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="306" mass="33800" checksum="FE7CC8920A737E31" modified="2017-09-27" version="1">MWGFELELKKSIRTKKFWLILVLILLIYVMAFREVKDNLEGASDPQGVLLTSLVGYIAVSAFLFIGVYALMAGATGINSDLENGTLRVALSKPLGRGSYLIGKFLGQSVSIVVAMVFATLLSFVVTKHYGLSLSGKLALDLALANGLVLLAMLQLLALGLLISTFVRSPNTALGLALVLFFVTGLVAPQIVDGWAEDRADKEFGLHERGDFTKLSPEQRTAYRERLGELYREYHLKYLFYVPQVLMLDIISGVETSKFNEDGTYTIEYLGLRHAISENPAQTGVIAALIPVYLALALIRFKRMDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P694</accession>
    <name>A0A218P694_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">A-type ATP synthase subunit B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">atpB</name>
        <name evidence="12" type="ORF">A3L08_02605</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane. The B chain is a regulatory subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853560.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P694"/>
    <dbReference type="GeneID" id="33315127"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02605"/>
    <dbReference type="OrthoDB" id="32941at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18112">
        <property type="entry name" value="ATP-synt_V_A-type_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18118">
        <property type="entry name" value="ATP-synt_V_A-type_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01135">
        <property type="entry name" value="V_A-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00310">
        <property type="entry name" value="ATP_synth_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005724">
        <property type="entry name" value="ATPase_A1-cplx_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022879">
        <property type="entry name" value="V-ATPase_su_B/beta"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01041">
        <property type="entry name" value="ATP_syn_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003235">
        <property type="entry name" value="PRK04196.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389:SF4">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039114">
        <property type="entry name" value="V-ATPsynth_beta/V-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 8" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="5 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="10">
        <location>
            <begin position="9"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="9">
        <location>
            <begin position="132"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase A/B type C-terminal" evidence="11">
        <location>
            <begin position="355"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF22919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="462" mass="51874" checksum="B26C10E42B58D547" modified="2017-09-27" version="1">MPGMEYSTVSKIYGPLMIVQGVKGVAYGEVVEIETESGEKRKGQVLEAREDMAIVQVFEGTRDLDVKSTRVRFTGETLKVPVSMDMLGRIFNGIGKPIDGGPEIIPEDRRDVHGAPLNPVARAYPRDFIQTGVSAIDGMNTLIRGQKLPIFSGSGLPHNMLAAQIARQAKVLGEEESFAVVFAAMGITYEEANFFKKSFEETGAMERAVLFLNLADDPAIERIITPRMALTVAEYLAFDYDMQVLVILTDMTNYAEALREISAAREEVPGRRGYPGYMYTDLATIYERAGRVRGRKGSITQVPILTMPDDDITHPIPDLTGYITEGQIVLSRELHRKGIYPPIDVLPSLSRLMKDGIGKGMTREDHPQLSQQLYAAYAEGRSLRDLVAVVGEEALSETDRKYLKFADRFEREFVAQRYDEDRSIFETLDLGWELLAELPESELKRVRKEYILKYHPKYRKGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6B2</accession>
    <name>A0A218P6B2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA primase large subunit PriL</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">priL</name>
        <name evidence="10" type="ORF">A3L08_02730</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Regulatory subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. Stabilizes and modulates the activity of the small subunit, increasing the rate of DNA synthesis, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="7">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimer of a small subunit (PriS) and a large subunit (PriL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the eukaryotic-type primase large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853584.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6B2"/>
    <dbReference type="GeneID" id="33315152"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02730"/>
    <dbReference type="OrthoDB" id="46081at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06560">
        <property type="entry name" value="PriL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00701">
        <property type="entry name" value="DNA_primase_lrg_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058560">
        <property type="entry name" value="DNA_primase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023642">
        <property type="entry name" value="DNA_primase_lsu_PriL"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003051">
        <property type="entry name" value="PRK03968.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04104">
        <property type="entry name" value="DNA_primase_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26466">
        <property type="entry name" value="DNA_primase_lrg_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140914">
        <property type="entry name" value="PriB N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="6 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 7" id="KW-0639">Primosome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA primase large subunit C-terminal" evidence="9">
        <location>
            <begin position="236"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="391"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="391"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="413" mass="47861" checksum="545FC24A9A42ABCE" modified="2017-09-27" version="1">MLDPFGAEARRLVKEEFGGITELLMVIPSYVGLETALERVRWVDSGKIPEEVLELDGIRDLLTFYALLGALAFSPYGLEMELVREANLRIYSARIERARTLEGTSLPLETVRGDEIPERDRIILERTHHTELSQEERRKLRLEYRLSLSKFLELWDGSLKEVYVRNGYAYLTWNQSVELWRRSFERSFERAVNLLYEVRDELPAYYLQLYERLGEIAREHFKERLEKLGRAEAQPLRFDLFPPCVKIALGGVPSGLRNYAITVLLTSFLSYARLCPNPPRRDVRVRDCVQSLDVIEKEILPVIIEAGNRCSPPLFEDQPHEIKNIWYHLGFGLTDKPTLEDSGNSPWYFPPNCSKIRANAPELCKPDRDCRNIKNPLTYYLRKLYFENKKSKPQETSKENTGEAAGAGVRDNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6D1</accession>
    <name>A0A218P6D1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Amylopullulanase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_02805</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the glycosyl hydrolase 57 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853599.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="GeneID" id="33315167"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02805"/>
    <dbReference type="OrthoDB" id="18576at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09626">
        <property type="entry name" value="DOMON_glucodextranase_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd10796">
        <property type="entry name" value="GH57N_APU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1190">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027552">
        <property type="entry name" value="CGP_CTERM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052046">
        <property type="entry name" value="GH57_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019248">
        <property type="entry name" value="Glucodextran_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004300">
        <property type="entry name" value="Glyco_hydro_57_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04288">
        <property type="entry name" value="CGP_CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36306:SF1">
        <property type="entry name" value="ALPHA-AMYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36306">
        <property type="entry name" value="ALPHA-AMYLASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09985">
        <property type="entry name" value="Glucodextran_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03065">
        <property type="entry name" value="Glyco_hydro_57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49344">
        <property type="entry name" value="CBD9-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1323"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 57 N-terminal" evidence="6">
        <location>
            <begin position="37"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Glucodextranase-like C-terminal" evidence="7">
        <location>
            <begin position="794"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="domain" description="Glucodextranase-like C-terminal" evidence="7">
        <location>
            <begin position="1038"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1275"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1279"/>
            <end position="1320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="1347" mass="150625" checksum="D53962A637B9ADB5" modified="2017-09-27" version="1">MKRQVVALFLAFLMVGSIVGVNLKTVGAAEPKPLNVIIVWHQHQPYYYDPVQDIYTRPWVRLHAANNYWKMAHYLSEYPDVHVAIDLSGSLIAQLVDYMDGKRDNYQIITEKIANGEPLTVDEKWFMLQAPGGFFDHTIPWNGEPITDPNGNPTRDFWDRYTELKDKMLQAKAKYANLPLEEQKAAVTGEFTEQDYIDLAVLFNLAWTDYGYIMDNPELKAIYEKVDEGGYTRDDVKTVLDAQMWLLNHTFEGHEKINYLLGNGNVEVTVVPYAHPIGPILNDFGWENDFDEHVKVSHELYKEYLGGGSVTPVGGWAAESALNDKTLEILAENGWTWVMTDQLVLDRLGVEKTTENYYKPWVAEFNGKKIYLFPRDHALSDRVGFTYGGMNQYQAVEDFVNELLKVQKENYDGSLVYVVTLDGENAWENYPYDGKLFLNELYKKLEELQEQGLIRTVTPSEYIKLYGEEANVLTPKMMERLDLTGDKVEALLKAQSLGDLYDMVGVKEEMQWPESSWIDGTLSTWIGEPQENYGWYWLYQARKALMEHKGEMSQADWEKAYEYLLRAEASDWFWWYGSDQDSGQDYSFDRYLKAYLYEMYRLAGLEPPSYLHGNYFPDGEPYQKLGPIGLKKGEVQSYSSLSPKASSVSVYFDDRGVHFVLNGDVDEFEVSLYEARKRVGNTFTLLQKKPDQLRYSLWPFSADSVGLMITKHVVYRDGKAEIYNATGYEESEKLGDLTVEKSGNTVDVTVPFEYIETPNDFYFAASTVDGNGNLDIISTPVELKLPTEVKGVVIADVKDPEGDDHGPGTYTYPTDGVFKPGVFDLLEFKLLEQTDAYVMQFKFKDLGGNPWNGPNGFSLQIIEVYFDYKDGGNSSAIKMFQDGPGSNVNLDPDHPWDVAFRIAGWDYGNLIVLPDGTAIQGEMQISADPASNTITVKVPKKYIQINEDYGLWGDVLIGSQDGYGPDKWRVVAVDAEQWKLGGADPQAVINGVAPRVVDELVPEGFKPTQEEQLSSYDANGGKLATVRAIPLLKQGIVVNDPEGDDHGPGTYTYPTDGVFKPGVFDLLRFKMTEGDDAWTLEFYFKDLGGNPWNGPNGFSLQIIEAYFDYTEGGNVTAIKMFPDGPGSNVQLDPRHPWDVALRIAGWDYGNLIIMPDGTVYQGEMQISADPTKNAIIVKLPKKYLPGVGDYGLYASVLVGSQDGYGPDKWRVVAVEAEQWKLGGAEPDAVINGVAPRVVDELVPEGFKPTQEEQLSSYDAENMKLATVVMIPLVEGSGGEVTPTPTETTSSTSSTSSTTSETTTSPSSTTQPTTNSPTTTTTGGGGGICGPAALIGLAIIPLLLRRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6G5</accession>
    <name>A0A218P6G5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Magnesium chelatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_02990</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853631.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6G5"/>
    <dbReference type="GeneID" id="33315204"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02990"/>
    <dbReference type="OrthoDB" id="24581at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000640">
        <property type="entry name" value="MoxR family ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.80">
        <property type="entry name" value="Magnesium chelatase subunit I, C-Terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011703">
        <property type="entry name" value="ATPase_AAA-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050764">
        <property type="entry name" value="CbbQ/NirQ/NorQ/GpvN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041628">
        <property type="entry name" value="ChlI/MoxR_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42759:SF1">
        <property type="entry name" value="MAGNESIUM-CHELATASE SUBUNIT CHLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42759">
        <property type="entry name" value="MOXR FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07726">
        <property type="entry name" value="AAA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17863">
        <property type="entry name" value="AAA_lid_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002849">
        <property type="entry name" value="AAA_ATPase_chaperone_MoxR_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="30"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="306" mass="34538" checksum="6D2491821AC664D4" modified="2017-09-27" version="1">MILEKIQAEIRKALVGMEDIVELMTIALIANGHVLLEGVPGIAKTTLSKNFANTLGLKFSRVQMTPDLLPADLIGHTFYDMRTGEFKIRKGPIFANVVLVDEINRASPKTQSALLEAMEERQVTIEGLPLKLPEPFVVLATMNPVEMEGVYELPTAQVDRFMMRIDMSYLPEDTEKEMLRRKNLGQFSEAKQVVLQSELAQARRDAMKVHVSDAIIDYIYEVVKETRLDERVILGASPRAGEHLLYAAKVRAYLENRGYVIPDDVKWLAVPVMAHRIVVKPEYEVEGIDGKTIVRDVLERIEVPVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6H1</accession>
    <name>A0A218P6H1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_02890</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853613.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6H1"/>
    <dbReference type="GeneID" id="33315184"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02890"/>
    <dbReference type="OrthoDB" id="17861at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051258">
        <property type="entry name" value="Diverse_Substrate_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42920:SF5">
        <property type="entry name" value="EAMA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42920">
        <property type="entry name" value="OS03G0707200 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="7"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="140"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="278" mass="31060" checksum="676C826096589CCC" modified="2017-09-27" version="1">MNRSEAVLLGITAIWGFTFPAMKVSLDYLPPMLFLAYRFGIASFLMLILFRSKVLRKETFREGFLLGLTLFFGHGFQIVGLKYTTASNSAFITSLYVVFTPFIAYFLLGDRLRPRDAVSLVVALTGLYLISGASLSLNHGDLLTVLCALSFAFQIVLVQRFGEKDYLSLAFWQITWNFVFSLAFALLFEPLVIPTDPLPWAGILYTSIFATVIAFTLQVKHQRNTSAHKAALIYSSEPIFGHVAAFLTIGEVLSVEGYIGALLIMAAIWNEVRHDHFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6I8</accession>
    <name>A0A218P6I8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_03110</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853653.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6I8"/>
    <dbReference type="GeneID" id="33315228"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03110"/>
    <dbReference type="OrthoDB" id="46222at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43630">
        <property type="entry name" value="POLY-BETA-1,6-N-ACETYL-D-GLUCOSAMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43630:SF1">
        <property type="entry name" value="POLY-BETA-1,6-N-ACETYL-D-GLUCOSAMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="321"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="364"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="4">
        <location>
            <begin position="47"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="405" mass="46548" checksum="495217F28DCF3D86" modified="2017-09-27" version="1">MDVLAIVYIISGALILWHLLIYPIVMGIIALIKKPLEKDYSYLPKFSIIVPAYNEEKVIINRIKNLEALNYPKDRYEIIIVESGSTDNTYMKVEEYIKSRKSKFPIIKLLHEAKRKGKPSAVNYGKKYSDGEIILVTDANSIFDKNVLKELAPHFKDPKVGAVGGRFVPIKNNSQGEGVDFYWDWEFLMRLGESQLYSTCVMHGEINAWRKELVELNPNLISDDLMIPLEVISKGKLVKYEPNAVVYEKVPSTKVDEIKQRKKNAIGTIQVTFKYIKFLLSPRLYSLIYFSHKGLQIFFPYLIVSFALPLLVGLAKKNSNVLYLLALYSILSIVSLFILMIIIQKVKVTYDIGAQTNNAKASSLIIKLPAIVIYFLWLQWIVVLAWFDYLRGRYSVKWQKAESTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6J6</accession>
    <name>A0A218P6J6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.282</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">tRNA wyosine derivatives biosynthesis protein Taw3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">taw3</name>
        <name evidence="9" type="ORF">A3L08_03175</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wyosine derivatives biosynthesis pathway. Probably methylates N-4 position of wybutosine-86 to produce wybutosine-72.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36635"/>
            <dbReference type="Rhea" id="RHEA-COMP:10378"/>
            <dbReference type="Rhea" id="RHEA-COMP:10379"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73543"/>
            <dbReference type="ChEBI" id="CHEBI:73550"/>
            <dbReference type="EC" id="2.1.1.282"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the TYW3 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.282" evidence="7"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853661.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6J6"/>
    <dbReference type="GeneID" id="33315241"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03175"/>
    <dbReference type="OrthoDB" id="19299at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1960.10:FF:000010">
        <property type="entry name" value="tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1960.10">
        <property type="entry name" value="tRNA wybutosine-synthesizing-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00266">
        <property type="entry name" value="TYW3_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022908">
        <property type="entry name" value="Taw3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003827">
        <property type="entry name" value="tRNA_yW-synthesising"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036602">
        <property type="entry name" value="tRNA_yW-synthesising-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003266">
        <property type="entry name" value="PRK04235.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003267">
        <property type="entry name" value="PRK04235.1-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047731">
        <property type="entry name" value="tRNAMtaseTaw3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48418">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48418:SF1">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02676">
        <property type="entry name" value="TYW3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111278">
        <property type="entry name" value="SSo0622-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA wybutosine-synthesizing protein" evidence="8">
        <location>
            <begin position="11"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="195" mass="22626" checksum="08DECFEF5CEDFB7C" modified="2017-09-27" version="1">MFLYEKNFEEQKAMAMEGLRKALAEDKVDHDIIPLLEKINALDNYFTTSSCSGRISVMEMPHFGDKVNSVWLGKWHREVTVEEVLEAIRKHRSGQLWFLVRSPILHVAARTMEDAVKLLNLAIGLGFKYSNIKSVSHKKLLVEIRSTERMDVPLGENGEPWVSEEYIKRIVNLANAQLRRFKGKLKRFEEEIEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6K8</accession>
    <name>A0A218P6K8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03315</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853686.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6K8"/>
    <dbReference type="GeneID" id="33315269"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03315"/>
    <dbReference type="OrthoDB" id="85914at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="464" mass="51542" checksum="3EB3A3900575425D" modified="2017-09-27" version="1">MRRRGQILSLDAMLALVMVVVMLGTITSTSTALQNEISTMVSWYDRANIASNMLDVLTKNPGDPANWIKDASKLRSLGLRSDTYPYAVSYEKISALMQLGDDTAVVNSLISMSNNKDFELHLYLTNTTVSLTGNFPKRVFIDLSDGKDRNMQIGQGSTGNNPFDATNVTLNGDKLPKRNQPYSLSPGDVLAFYTLEDITVHDRKNGEDYPIPAPAYVGIQVISTGSHFQVQWTDRGLHITGQGQVRIIVEGYQKNTIQVNVDVTEPEELTAPSYRIAVINGSKVNDDATIQKSRDRSPWVEYIERKVTVEKLKYEESIDVDSPSTTEWIAGRLTMNVPEYAYFRVTVAPQDTGRIILIARDGDEYRGVLIEKQSEDSALQAVVATSGDSSPPKFYIGNTTSVDVPWSSIFQAFDTSTGSKVILVWIYGNTFGGTAKITDMGHLGTIMKPKFERTMLKLWVWDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6L1</accession>
    <name>A0A218P6L1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TIGR00529 family membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853665.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6L1"/>
    <dbReference type="GeneID" id="33315246"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03200"/>
    <dbReference type="OrthoDB" id="19102at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005253">
        <property type="entry name" value="CHP00529"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007294">
        <property type="entry name" value="DUF401"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00529">
        <property type="entry name" value="AF0261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39556">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39556:SF1">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04165">
        <property type="entry name" value="DUF401"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="340"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="372"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="395" mass="43607" checksum="8A9DFAA74EFFB5A6" modified="2017-09-27" version="1">MEVLYLVLSFAVVIALIWLKINIGLSIFAGSLVLAFLFGMSPPEIAVALYRSATSWETLRLVLIIASIMGMTSVVSQIGYLKDMERAASELFPKAKYSLAMLPALIGLMPMPAGALVSAPMIEPVAGRFEMDPSDKTLVNYWFRHVWEHSWPMYQAIVIASAIVGLSIREISTKMFPLTVLMALIGYLIFIRPLPDEGSGSGNRWAGFWLLLKSTYPILVIILISIVLGIDMVYGALLGFLSALLPNIKRVNVREVATHALQPKIVFLLISIMYFKYILEATGAVEALPKAMLAMNLPVILILMLTPFIVGLMTGISFAYVGMTFPLLLPFFKSFDAIALAYLSGYMGMLFSPVHLCLVFSAEYYGAELRRVYLKLLAPAAALFVLGLTYIFFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6L5</accession>
    <name>A0A218P6L5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Thermosome subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_03165</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone; binds unfolded polypeptides in vitro, and has a weak ATPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Forms a Heterooligomeric complex of two stacked eight-membered rings.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853659.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6L5"/>
    <dbReference type="SMR" id="A0A218P6L5"/>
    <dbReference type="GeneID" id="33315239"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03165"/>
    <dbReference type="OrthoDB" id="9362at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03343">
        <property type="entry name" value="cpn60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012714">
        <property type="entry name" value="Thermosome_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02339">
        <property type="entry name" value="thermosome_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="545" mass="58803" checksum="FE2E3DA5C0EEBEFE" modified="2017-09-27" version="1">MAQLAGQPVVILPEGTQRYVGRDAQRLNILAARIVAETVRTTLGPKGMDKMLVDSLGDIVITNDGATILDEMDIQHPAAKMMVEVAKTQDKEAGDGTTTAVVIAGELLRKAEELLDQNIHPSIIIKGYALAAEKAQEILESIAKDVDVEDVETLKKAAITSITGKAAEEEREYLAEIAVEAVKQVAEKVGDGYKVDLDNIKFEKKEGGAVSDTQLIKGVVIDKEVVHPGMPRRVEGAKIALINEALEVKETETDAEIRITSPEQLQAFLEQEEKMLREMVDKIKEVGANVVFVQKGIDDLAQHYLAKYGIMAVRRVKKSDMEKLAKATGAKIVTNVRDLTPEDLGEAELVEQRKVAGENMIFVEGCKNPKAVTILIRGGTEHVVDEVERALEDAVKVVKDIVEDGKILAAGGAPEIELAIRLDEYAKEVGGKEQLAIEAFAEALKVIPRTLAENAGLDPIETLVKVIAAHKEKGPTIGVDVFEGEPADMMERGVIAPLRVPKQAIKSASEAAIMILRIDDVIAASKLDKDKGGEGGGNDFGGDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Q8</accession>
    <name>A0A218P6Q8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Inorganic pyrophosphatase</fullName>
            <ecNumber evidence="7">3.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pyrophosphate phospho-hydrolase</fullName>
            <shortName evidence="7">PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ppa</name>
        <name evidence="8" type="ORF">A3L08_03385</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) forming two phosphate ions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>diphosphate + H2O = 2 phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="7"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853698.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Q8"/>
    <dbReference type="GeneID" id="33315283"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03385"/>
    <dbReference type="OrthoDB" id="134160at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00412">
        <property type="entry name" value="pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.80.10:FF:000003">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00209">
        <property type="entry name" value="Inorganic_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="INORGANIC PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00387">
        <property type="entry name" value="PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="178" mass="20808" checksum="9CB024A800EB49DA" modified="2017-09-27" version="1">MNPFHELEPGPEVPEVVYALIEIPKGSRNKYELDKKTGLLKLDRVLYSPFFYPVDYGIIPQTWYDDGDPFDIMVIMREPVYPLTIIEARPIGIMKMEDSGDKDWKVLAVPVEDPYFEDWKDIDDVPKAFLDEIAHFFQRYKELQGKVTTVEGWGNAEEAKKEILRAIELYKEKFGKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6T8</accession>
    <name>A0A218P6T8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein eS6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps6e</name>
        <name evidence="5" type="ORF">A3L08_03575</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853729.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6T8"/>
    <dbReference type="GeneID" id="33315320"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03575"/>
    <dbReference type="OrthoDB" id="7793at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00512">
        <property type="entry name" value="Ribosomal_eS6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001377">
        <property type="entry name" value="Ribosomal_eS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020924">
        <property type="entry name" value="Ribosomal_eS6_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003293">
        <property type="entry name" value="PRK04290.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003294">
        <property type="entry name" value="PRK04290.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11502">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01092">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01405">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="125" mass="13749" checksum="84C10C4F980785BA" modified="2017-09-27" version="1">MATFKLVISNPKNGIAKQVEISGEEAEKLIGKRIGDEVPASELGLNLTEIFGEEIPGDAKLRITGGTDKDGFAMRPDVHGPRRVKILVSRGPGFRPKERGERRKKTVRGNTISPEIVQVNMKLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6U1</accession>
    <name>A0A218P6U1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PadR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853766.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6U1"/>
    <dbReference type="GeneID" id="33315361"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03780"/>
    <dbReference type="OrthoDB" id="56053at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052509">
        <property type="entry name" value="Metal_resp_DNA-bind_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33169">
        <property type="entry name" value="PADR-FAMILY TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33169:SF14">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR RV3488"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator PadR N-terminal" evidence="1">
        <location>
            <begin position="14"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="99" mass="11450" checksum="5A14635583D7CAC7" modified="2017-09-27" version="1">MMRRVLLGFMGIHILHHASRGEVTGTFMMAELSRHGYKVSPGTIYPLLHKMEEMGLLRSRTEVRNGRRVRLYRATPKGEELLEEAREKVRELCTEILGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6V7</accession>
    <name>A0A218P6V7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MFS transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_03775</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853765.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6V7"/>
    <dbReference type="GeneID" id="33315360"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03775"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17370">
        <property type="entry name" value="MFS_MJ1317_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23518">
        <property type="entry name" value="C-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23518:SF2">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="307"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="344"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="371"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="14"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="394" mass="43476" checksum="70DC595F6E996CE1" modified="2017-09-27" version="1">MEEKERKIFGISWNVFLLGIVSFLNDMSSEMINPIVPSYLTGVLREGQLVSGSVMGAIESMSSLFKVAFGYVSDRFRKRKAFVLAGYALSTLAKGALAFTRYWWDFLTLRALDRVGKGIRTAPRDALIAESSEKGKSGKSFGFHRMMDTLGAVAGPLAAIALIELLRGLPKETMYRYIFLLSAIPGLLSLFVIVLFVRDRGSEVKKKIKGISTLRSRGLQLFLAVVAVGALGRYSYAFTLWKAEELGFTVVQGIAFYALFNLIYALSAYPLGVVSDSFGKKRLITIGFLVATLASLAFAYARDFYTLVVAFVLYGVYIAIEDTIPRAYMADLAGEHEKGTIIGAYHTVFGLFVFPASVIAGYLWQARSLELAFTYAALMNALAFVMMFFVPESH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6X2</accession>
    <name>A0A218P6X2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Exosome complex component Rrp41</fullName>
            <ecNumber evidence="9">3.1.13.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rrp41</name>
        <name evidence="12" type="ORF">A3L08_03860</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalytic component of the exosome, which is a complex involved in RNA degradation. Has 3'-&gt;5' exoribonuclease activity. Can also synthesize heteromeric RNA-tails.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Catalytic component of the exosome, which is a complex involved in RNA degradation. Has 3'-&gt;5' exoribonuclease activity. Can also synthesize heteropolymeric RNA-tails.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8 9">Component of the archaeal exosome complex. Forms a hexameric ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers. The hexameric ring associates with a trimer of Rrp4 and/or Csl4 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the RNase PH family. Rrp41 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.-" evidence="9"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853782.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6X2"/>
    <dbReference type="GeneID" id="33315377"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03860"/>
    <dbReference type="OrthoDB" id="24266at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11366">
        <property type="entry name" value="RNase_PH_archRRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000004">
        <property type="entry name" value="Exosome complex component Rrp41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00591">
        <property type="entry name" value="Exosome_Rrp41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011807">
        <property type="entry name" value="Rrp41"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02065">
        <property type="entry name" value="ECX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF0">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9 12" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5 9" id="KW-0271">Exosome</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="10">
        <location>
            <begin position="25"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="11">
        <location>
            <begin position="157"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="249" mass="27482" checksum="6AA8C94A3BDD2B09" modified="2017-09-27" version="1">MMGRPEGLKLIDENGKRLDGRKKYELRPIKMEVGVLKNADGSAYVEWGKNKILAAVYGPREIHPKHLQRPDRGILRVRYNMAPFSVEERKKPGPDRRSVEISKVIRGALEPALMLEMFPRTAVDVFIEVLQADAGTRVAGITAASLALADAGVPMRDLVAACAAGKIDGEIVLDLNKDEDNYGEADIPVAIMPLKNDITLLQMDGYLTKDEFVEAVRLAIKGAKAVYQKQREALKEKYLKIAEEVGGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Y6</accession>
    <name>A0A218P6Y6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03930</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853795.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Y6"/>
    <dbReference type="GeneID" id="33315391"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03930"/>
    <dbReference type="OrthoDB" id="85637at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd11714">
        <property type="entry name" value="GINS_A_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21697">
        <property type="entry name" value="GINS_B_archaea_Gins23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038437">
        <property type="entry name" value="GINS_Psf3_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="164" mass="19066" checksum="133F768F2607D83C" modified="2017-09-27" version="1">MFTGRAVIAVKILRPFGDWKTGDIVLIEDWKAKELWEAGVVEVVDETDKVIGEIDKAIAEERSNEPLMPLPAGLYERAAFYMYYLENYVRLNAGESIETINVKLTKLANLKKKTEHLKTIRFNKILKAVMWRPNSLELLSRLSPEERRVYLQLSKIRNEWLGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Z0</accession>
    <name>A0A218P6Z0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_04040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853814.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Z0"/>
    <dbReference type="GeneID" id="33315412"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04040"/>
    <dbReference type="OrthoDB" id="4144at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18086">
        <property type="entry name" value="HsC9orf114-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003750">
        <property type="entry name" value="Put_MeTrfase-C9orf114-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12150">
        <property type="entry name" value="CLASS IV SAM-BINDING METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12150:SF13">
        <property type="entry name" value="METHYLTRANSFERASE C9ORF114-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02598">
        <property type="entry name" value="Methyltrn_RNA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="256" mass="29192" checksum="90CA9F8486E766C2" modified="2017-09-27" version="1">MAWHVFIPDSLLEESDDPKIRTYKVGQITRACAIFGVEHIWIYRAGGRDGRFIKTILEYAETPQYLRKRLFPLMPELKYAGVIPPLRTPHHKLKGKPKVGEIREGFAFRKGRRIYADIGLDDLAMVEGNVEGRATFRIVSVRPLRVIPAKPEEYWGYRVHLTGKSLAKTLKKARLDLVIATSRKGRDIREVKLPPLEGEVGFVFGSPRKGVIELLGEEDYTFDLILNTVPNQRTATVRTEEALLATLAIFNLIRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Z2</accession>
    <name>A0A218P6Z2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CBS domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_03895</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853789.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Z2"/>
    <dbReference type="GeneID" id="33315384"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03895"/>
    <dbReference type="OrthoDB" id="85195at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="7"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="67"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="133"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="228"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="281" mass="31966" checksum="66F928D7DD284ABF" modified="2017-09-27" version="1">MRVKTLMTKDPVVIELPATREYALELFRKHKVRSFPVINKNTKSLVGIISIKRVLLHPDEDQLAMLIRRDVPVVKPDDDLKKAVRAMLEMDYRRVIVVDDENRVLGILTVGDIVRRYLAKNEKLKEVTIEPYYQRNVGVVWRGTPLKAALKALLLSNSMAIPVIDDEGNLVGMVDETDLLKDSEVVRIMKQTSLAALSEEEWMLESNPTLLFEKAELQLPKKPVGEIMNPNVVVATPHMSVYDVAQKMVQYHIEQLPVIRGEGELVGIVRDMDIIKVILNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P703</accession>
    <name>A0A218P703_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl3</name>
        <name evidence="8" type="ORF">A3L08_04045</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 50S ribosomal subunit. Forms a cluster with proteins L14 and L24e.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853815.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P703"/>
    <dbReference type="GeneID" id="33315413"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04045"/>
    <dbReference type="OrthoDB" id="6121at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1430.10:FF:000005">
        <property type="entry name" value="50S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.960.10">
        <property type="entry name" value="Ribosomal protein L3, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01325_A">
        <property type="entry name" value="Ribosomal_uL3_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045077">
        <property type="entry name" value="L3_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044892">
        <property type="entry name" value="Ribosomal_L3_dom_3_arc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_uL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019928">
        <property type="entry name" value="Ribosomal_uL3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_uL3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03626">
        <property type="entry name" value="L3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003261">
        <property type="entry name" value="PRK04231.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="328"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="347" mass="39593" checksum="23F9880D3033F8E8" modified="2017-09-27" version="1">MGKIHRPRRGSLAYSPRKRAKSIVPRIRKWPKDSEVRMLGFAGYKAGMTHVLMIDDRPGLTKGKEIFMPVTIVEVPPLFVYGIRAYRQGYLGLETATEVWFHELNDHVKRRIKTLPKDYNEDAFQAKLGQLEDLVNDGEIVDVRLLVHTQPWLIKLKKKPEVMEYAIGGDDVKAKFAYAKEKIGKEIRASEVLHEGELLDIIAVTKGKGTQGPVKRWGVKIQFHKAQRAGKARHIGNLGPWHPTRVMWTVPQAGQMGFHHRTEFNKRLIAIGENGKLKLDEKNEVDITPKGGFPHYGIIRSDFLMIQGTVPGSFKRIVRVRPAIRAPKKRPPVERPQITYVSRESKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P725</accession>
    <name>A0A218P725_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_04195</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853843.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P725"/>
    <dbReference type="GeneID" id="33315443"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04195"/>
    <dbReference type="OrthoDB" id="7442at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44196">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE SDR FAMILY MEMBER 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44196:SF1">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="236" mass="26573" checksum="D123ECBF027DEC5D" modified="2017-09-27" version="1">MKTALITGPTGGLGRLLVEKLIERGYRVVGVARNEEKLRKLRDELLSFEYIVADLSKENFSDTVLEGLGRLGVERVDLLVNNAGFALRKPLLEHSREELENLFRVNTLAPVELTKALIPMLPKGSTVVFVISGVAFISVPELPSYCAAKAALHYLTINLERELGARGIHVMRVYPKQVKTSFWNGRVPKGSIEPQRVVDAILRGLDKGKREVFVPGYLKLVKYLPNWPVFTYHFKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7A2</accession>
    <name>A0A218P7A2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Biotin--protein ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_04665</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853920.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7A2"/>
    <dbReference type="GeneID" id="33315537"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04665"/>
    <dbReference type="OrthoDB" id="121492at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38075">
        <property type="entry name" value="DUF4139 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38075:SF1">
        <property type="entry name" value="DUF4139 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="434" mass="48156" checksum="D3E4E84A4ADF2FE2" modified="2017-09-27" version="1">MKKKAIAALGGIMLLLFAVFSFQGEKAAVGDTTVVLYSSARIGVIEETKEVELKEGLNEVPLNELAGLNIAEVTVMPLDDGVRVLGVFSRGSNGDVYSANVGSEVEIKLRSGGTITGKFLGFKDGKITVEGDGYYLVNPDEVSYFKAKNLEGTASVYAVLQADNAGRYNVSVTYRVANMSWESRYKLYIGDNAKLYGYIVLNNPTAQEFKDAKVLLVAGDVQLYQALPQPRTLYAKAEAGVEAVEVSQPQKVEAFYLYRLGIVDLNPSSTMMYPYISMEAPFEREYLYESWPGNGERPVYESIAFKTDKVLPEGIVEIYRETSDGALLIGERGIEHAPKGDTLRIGIGRDYDLKGTTTVLEQKNGDGYAYYKIKVTLENFGNETKTIIVRHHKWGKIITSSVQPVDETANYVEFSVTLKPGEREEIVFDYENRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7D1</accession>
    <name>A0A218P7D1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">C4-dicarboxylate ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_04780</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7D1"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04780"/>
    <dbReference type="OrthoDB" id="371890at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010656">
        <property type="entry name" value="DctM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011853">
        <property type="entry name" value="TRAP_DctM-Dct_fused"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02123">
        <property type="entry name" value="TRAP_fused"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43849">
        <property type="entry name" value="BLL3936 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43849:SF2">
        <property type="entry name" value="BLL3936 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06808">
        <property type="entry name" value="DctM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="444"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="472"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="495"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="540"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="575"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="600"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="627"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="711"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="745"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="domain" description="TRAP C4-dicarboxylate transport system permease DctM subunit" evidence="2">
        <location>
            <begin position="156"/>
            <end position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="777" mass="84899" checksum="6B829ED8BFE6A41C" modified="2017-09-27" version="1">MPDIDKEPKGLEELEELKEMEEKVEEVFEKTRTLTPKLELLIKAAAILIGIYEILFIFNFNYTLYDMFARMGLKIDLLRVTLQTKQGEAFVLAMILLITFLIYPLRKREEELRKVPFYDYILAALGVISSLYLFVVYQRYAEFAEVYTLDVVFGVLAIILVLEATRRVLGWVLPLVVTIFLLYGIQNIGFNWVRFTQQLYFDEGIFGIPFFVMTIYVFAFVFFGAFLLKIGISDYITEFMITLFGKRPGGPAKAAVVSSGLMGTVSGSSVANVLTTGTFTIPLMKKAGYPPEIAGAVEPVASTGGQLMPPIMGAAAFIMAEFLGVPYNKLIIAAVLPALVYYTGVYLFIDLETKRLGLKGMPSEHFAPLKYFLRKLYILLPIAVITVALVWGIPPHISAISSLGIAIWVAWIAKDEIEGHEGLYVGLVLLTTVLMFTSKSIAVPVAGILLLTGLALVALAFVSDLVELNEKLYISLLFILFVALTKFLGMSKEQILLMSGVMGIVFSLIVGYVSKSESGKKMYEATYQSMIDAGKTSTSVMLAAASAGLIQGVLTMTGLVTSLGYRLVDLTAGNLWLLLLLTMIFSLILGMGVPTTANYIITSLVAAPAIYNAVLGLQPYSSPVPGFGTPIALLAAHFFVFYFGILADVTPPVALASYAGSALAGGDFWKTAMNAVKYALAGYIGPYIYFTHPEMFIITVQEWTARMALTVIYDFGATLLVMYILAISLTGWFGVHIRKELRALLAAVGVLSATLNPIIVGIGLAAVLAIKFLGKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7G2</accession>
    <name>A0A218P7G2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit Rpo1C</fullName>
            <ecNumber evidence="8">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit A''</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rpo1C</name>
        <name evidence="8" type="synonym">rpoA2</name>
        <name evidence="11" type="ORF">A3L08_05045</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Forms part of the jaw domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853980.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7G2"/>
    <dbReference type="GeneID" id="33315613"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05045"/>
    <dbReference type="OrthoDB" id="372142at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06528">
        <property type="entry name" value="RNAP_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00411">
        <property type="entry name" value="RNApol_arch_Rpo1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012757">
        <property type="entry name" value="RPO1C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02389">
        <property type="entry name" value="RNA_pol_rpoA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF32">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 8 11" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNA polymerase Rpb1" evidence="10">
        <location>
            <begin position="28"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="21"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="391" mass="43895" checksum="371DA5380FB77BA6" modified="2017-09-27" version="1">MVAAKTIRSLVDKAELPENIKEELYNKLVEYDKKYKLKKAEIEAIIQEAVREYEKAKIEPGEAIGTVAAQSIGEPSTQMTLNTFHYAGVAEINVTLGLPRIIEIVDARKNPSTPIMTVYLDEKHRYDREKALEVARRIEGTTLENLARETSIDILNYEFIVEIDPERLQKAGLDMEKIQRKLEGSFKTAEFEVDGYTLIMRPKKVGKLSDLRKLSEKVKKHRLKGLSGVGKTIIRKEGDEYVIYTEGSNFKQVLKVPGVDPTRTRTNNIWEIAEVLGIEAARNAIIEEIVNTMREQGLEVDVRHIMLVADMMTLDGVILPIGRHGIVGEKASVLSRASFEITTQHLFEAAERGDVDPLNGVVENVLIGQPVPVGTGMVKLAMNLPLRPKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7G6</accession>
    <name>A0A218P7G6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable transcription termination protein NusA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nusA</name>
        <name evidence="9" type="ORF">A3L08_05055</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Participates in transcription termination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NusA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853982.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7G6"/>
    <dbReference type="GeneID" id="33315615"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05055"/>
    <dbReference type="OrthoDB" id="4116at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22530">
        <property type="entry name" value="KH-II_NusA_arch_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22531">
        <property type="entry name" value="KH-II_NusA_arch_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.20:FF:000024">
        <property type="entry name" value="Probable transcription termination protein NusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00945_A">
        <property type="entry name" value="NusA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010212">
        <property type="entry name" value="NusA_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030842">
        <property type="entry name" value="TF_NusA_bacterial"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01952">
        <property type="entry name" value="nusA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22648">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR NUSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22648:SF0">
        <property type="entry name" value="TRANSCRIPTION TERMINATION_ANTITERMINATION PROTEIN NUSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 6" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="32"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="145" mass="16253" checksum="CD280383ADA36384" modified="2017-09-27" version="1">MPLKLNTDQIKFIALFESMTGATVLDCLIDTGRNRLIYVIKKGEMGLALGKKGANVKRVQNMLGKDIELIEHSDNPEEFLRNIYKSLGVKVKKVHITEKRDGKKVALLDIGPRDKPRAIGRGGQNINLVKELMERHHGISDVIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7H4</accession>
    <name>A0A218P7H4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_04990</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853973.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7H4"/>
    <dbReference type="GeneID" id="33315602"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04990"/>
    <dbReference type="OrthoDB" id="84852at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="94" mass="10592" checksum="F6F9B49D4B6BD728" modified="2017-09-27" version="1">MAYIGFVKSPYGPGRTFDAIIDELKRQGFTIGFSKHHWAGDLPFGLVIAETDKGPVAIRWSLGKEFSIRLEEVDKETYDEFVEDTIEYTNADSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7I4</accession>
    <name>A0A218P7I4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Alanine aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_05115</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853990.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7I4"/>
    <dbReference type="GeneID" id="33315627"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05115"/>
    <dbReference type="OrthoDB" id="372018at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051926">
        <property type="entry name" value="Ala_Aminotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006230">
        <property type="entry name" value="PRK08363.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43488">
        <property type="entry name" value="GLUTAMATE-PYRUVATE AMINOTRANSFERASE ALAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43488:SF2">
        <property type="entry name" value="GLUTAMATE-PYRUVATE AMINOTRANSFERASE ALAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="5">
        <location>
            <begin position="31"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="399" mass="45291" checksum="47DDF5A817CCE730" modified="2017-09-27" version="1">MIRASERAMGIEYAIRDVVLPARELEKKGIKVIRLNIGDPGKYDFQPPKHMRDAYCKAIQEGHNYYGTSEGLPELREAIVQREKRKNGVDITPEDVRVTAAVTEALQFIFGALLNPGDNILVPSPSYPPYTGLVKFYGGVPNEYLTVEENGWQPDIDDMRKKINEKTKAIAVINPNNPTGALYEKKTVREILDLAGEYGLPVISDEIYDLMTYEGEHVSPGSLTKDVPVIVMNGLSKVYFATGWRLGYFYYVDPEDKLSEVREAIDKMARIRICPNTPAQFAAIAGLTGPMDYLKEYMAKLKERRDYIYKRLTEIPGISTTKPQGAFYIFPRIDERSKWKNDKEFVLDALNEAHVLFVHGSGFGYAGDWHFRIVFLPPVPILEEAMNNFEAFMRRKLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7J5</accession>
    <name>A0A218P7J5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conjugal transfer protein TraB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05205</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854006.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7J5"/>
    <dbReference type="GeneID" id="33315645"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05205"/>
    <dbReference type="OrthoDB" id="185689at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd14726">
        <property type="entry name" value="TraB_PrgY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002816">
        <property type="entry name" value="TraB/PrgY/GumN_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046345">
        <property type="entry name" value="TraB_PrgY-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21530">
        <property type="entry name" value="PHEROMONE SHUTDOWN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21530:SF7">
        <property type="entry name" value="TRAB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01963">
        <property type="entry name" value="TraB_PrgY_gumN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="231" mass="26466" checksum="663E4D359D36D513" modified="2017-09-27" version="1">MSYLRYVRVIGTMHVSPKSREEVIRTILEERPHAVAVELDRTRFIAMGQEREMTFEDALRFGRRGLINYVLAKVEEKLGEEFGMKPGEEMKAAISTARALGVPLYLIDEDINVILGKIAAAPAREKLLMALEALAVFLPIKPGEGMPDPMSEYRTMMMGFKRRYPYLYRVLVEERNEVMVRNLISIVDNLKLQGIKRPRVVAVVGLGHKQGIEHILDRAEERVATSRYFYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7J8</accession>
    <name>A0A218P7J8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cobalt transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_05220</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854009.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7J8"/>
    <dbReference type="GeneID" id="33315648"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05220"/>
    <dbReference type="OrthoDB" id="269083at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="entry name" value="Cation efflux protein, cytoplasmic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050681">
        <property type="entry name" value="CDF/SLC30A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11562">
        <property type="entry name" value="CATION EFFLUX PROTEIN/ ZINC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11562:SF17">
        <property type="entry name" value="RE54080P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="Cation efflux protein cytoplasmic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="9">
        <location>
            <begin position="16"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein cytoplasmic" evidence="10">
        <location>
            <begin position="209"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="299" mass="33403" checksum="D9EC41117D10BCBC" modified="2017-09-27" version="1">MGHHHHHGELKGRMAFSIALNLVITIAEVIGGILSGSLSLLSDSLHNFSDSMSLLASYFAIKIGERRANEKYTFGYKRAEILVAFINSAVLVGVSLFLLVEAYRRFKNPEPIDGPLMLGVALIGLGANLISVLLLHSHAHESMNVRSAYLHLMSDTLSSVAVVVGGVLIIKWNVTWVDPLVTVFISLYILREGYEILKESVEVLMEASPELDFEVIKREIESIPGVKNAHHFHAWRIGEKEIHFECHVEVDDMPVSKGQAIIDEVEERLRKYGITHVTVQLEVNRCTSKNVICNEEKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7L9</accession>
    <name>A0A218P7L9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ADP-dependent glucose/glucosamine kinase</fullName>
            <ecNumber evidence="9">2.7.1.147</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ADP-dependent glucokinase</fullName>
            <shortName evidence="9">ADP-GK</shortName>
            <shortName evidence="9">ADPGK</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Glucosamine kinase</fullName>
            <shortName evidence="9">GlcN kinase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">glkA</name>
        <name evidence="10" type="ORF">A3L08_05185</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the ADP-dependent phosphorylation of D-glucose to D-glucose 6-phosphate and glucosamine to glucosamine 6-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-glucosamine + ADP = D-glucosamine 6-phosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:62084"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58723"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-glucose + ADP = D-glucose 6-phosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11460"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.147"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Carbohydrate degradation; glycolysis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ADP-dependent glucokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.147" evidence="9"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854002.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7L9"/>
    <dbReference type="GeneID" id="33315641"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05185"/>
    <dbReference type="OrthoDB" id="124916at2157"/>
    <dbReference type="UniPathway" id="UPA00109"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043843">
        <property type="term" value="F:ADP-specific glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1110.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00809">
        <property type="entry name" value="ADP_glucokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007666">
        <property type="entry name" value="ADP_PFK/GK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015990">
        <property type="entry name" value="ADP_PFK/GK_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031299">
        <property type="entry name" value="GlkA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010641">
        <property type="entry name" value="PRK14038.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21208">
        <property type="entry name" value="ADP-DEPENDENT GLUCOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21208:SF1">
        <property type="entry name" value="ADP-DEPENDENT GLUCOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04587">
        <property type="entry name" value="ADP_PFK_GK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015883">
        <property type="entry name" value="ADP-Pfk_glckin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51255">
        <property type="entry name" value="ADPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 9" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="7 9" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 9 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="440"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="112"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="342"/>
            <end position="343"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="454" mass="50700" checksum="50C57EE8E21797E1" modified="2017-09-27" version="1">MMTWDALYSSAFEAVRSRIGRVGRVLLAYNTNIDAIKYLDGRDLEGRIEKAGKDEVLRYADGLPERIENVPQLLGSILWSIKRGKAAELFVESCSTRFYMKRWGWDELRMGGQVGIMANLLGGVYGVPVIAHVPQLSKLQADLFKDGPIYVPKVENGSLKLVQPRNFAGDEENCFHYIYEFPRGFGVFDFEAPRENRFIGSADDYNPNVYIRPEFRERFGEIASEAELAVISGLQALTKENYRQPFEIIEEHLKVLNEKGIPVHLEFAFTPDETVRKAILGLLGKFWSVGLNEVELASIMEVMGEKSLAEKLLSHDPVDPIAVTEAMLKLAEKTGVKRIHFHTYGYYLALTDYKGDFVRDALLFAALAAAAKAKLGDVRSIDDVIKAMDVPVNEKAKPVEEALVREYGMRDGIAEVNGYQLAFIPTKIVAKPKSTVGIGDTISSSAFVGEFALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7N5</accession>
    <name>A0A218P7N5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Pyrolysin (Pls)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_05400</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854044.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="GeneID" id="33315684"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05400"/>
    <dbReference type="OrthoDB" id="27270at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF11">
        <property type="entry name" value="CEREVISIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S8/S53" evidence="7">
        <location>
            <begin position="182"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="379"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="1428" mass="158110" checksum="6A389A9A3EA40227" modified="2017-09-27" version="1">MNRKALSLLIVAVMVLSIVPVMLPEISVSAASADNLKDTPTSVASSTTNTNFVKGEALQKEIQRILQTSDRTVRLIVAPDREHAMEVYNALKDLGRIDPISRPEYQFIVIEMPVSNVEKLADIPGILHVWKDRMVKLQEPVSPEENVLAESPAKESPKLPDMFMSVFTINAYNVWDNYGIYGDNVIVAILDTGIDVAHPFLQTTLDGRPKIIHIKDMSDEGMVEIYHTTTTVSGGVISVNKDVTVYWGAYANYYGHDPITTYHMGDYVVGSISGDVYYLGLLPERYFDLNNFSGVPNDPYGFGLFGDLSDVYPVLIVNQSGNLFAYIDFNLNNDFTDEEPMGIYDLTRDYVVVSTTNVTVAFEGFYGNVAYFMWDAHGHGTHVSGTVAGVGLPSDPVFYGVYGVAPNAQLMEYKVLPGEQGFGRTSWIINGMIEATLDGADVISMSLGGGGEINDGLETPEIFYVNLLSDYYGVVFAIAAGNEGPTTNSVHAPGDSDLAITVGNYWESERWELLYGLPGVANGPAMGSSRGPRDDGLLDPDVMAPGTDIFSSLPMWYTVLYEDPYRYYGFWSGTSMATPHVSGAVALMISYAKARGIPYDPIMIKRALELSAKPTNQTLVDQGFGLIQVDKAIAKLEELSQEPSIYIFAGTTFTSFKNPIEVPLIPISQAYVDFNGYFQSVFGFPYLYRGVYIRDEFPGSVPIYFSPLEYWQGWGLGYVFENKMYTISTNVDWIIPNTTEVTIHGADARYIEDLMGQFSISIDYSRLQKSGTYVGLVYIDDPDTSYIDGYVPVIVDIPLNRNGELSATISDTANPGETKHYFFNVPRGTKELRITLRIPTDSEGTPMGRVKLVIARPLGEVVYDGVPGYLYAGPGGPLEYTWIVENPVEGNWEITAYVSVSSYASTGYEKAHYEIEVMTSDVEMNPELIVKDIAEPGTINVQAQVINNYETFNAEPVGYGMGRLDIAEGTMSQVGQDEVDIVGYAGVDPTTYFLKMGITDPEDPAADLDLYVAYYTTYEQMVNDLQDGYINDYTTLYTDQIGPTSEEVLELFMPDPGYYLVYVYGYDTAGHDKITYTFYYQILGDSGDISIQSEPFEFMKGAKETLEASVSISDEGTYLGIIGLRNSDTGEILSYAPIIIQAGKPSMTMVFSTNATIGKGSVIKIKFIDPVTDEPIKGETKVVVNGVPYYTDDGTLTVYYVPKHLEETFTINAINSQYQDIETQIAVKAKEPFDSTIEYTTRITPKVISGFGQVASYETYGNRLRITVDGPSGYEGYVLVPLPYSSGYAKVEGEGVVDYYYEVGSMATYLIVKVKYGSPVTFTVDFVPVKPAVSGINYFYYSRYLRLNQTFTELYQKAVELGVDNETLQMALNYQQAAEAWYKEGLEYMNPMATSSGVLAMIRFRRAYVSIQDAIRILEKAVEELQAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7N8</accession>
    <name>A0A218P7N8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phosphoenolpyruvate synthase</fullName>
            <shortName evidence="12">PEP synthase</shortName>
            <ecNumber evidence="12">2.7.9.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Pyruvate, water dikinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">A3L08_05415</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>pyruvate + ATP + H2O = phosphoenolpyruvate + AMP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11364"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.9.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.7.9.2" evidence="12"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854047.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7N8"/>
    <dbReference type="GeneID" id="33315687"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05415"/>
    <dbReference type="OrthoDB" id="23397at2157"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008986">
        <property type="term" value="F:pyruvate, water dikinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000010">
        <property type="entry name" value="Phosphoenolpyruvate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.10">
        <property type="entry name" value="Phosphohistidine domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008279">
        <property type="entry name" value="PEP-util_enz_mobile_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006319">
        <property type="entry name" value="PEP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018274">
        <property type="entry name" value="PEP_util_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000121">
        <property type="entry name" value="PEP_util_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023151">
        <property type="entry name" value="PEP_util_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036637">
        <property type="entry name" value="Phosphohistidine_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002192">
        <property type="entry name" value="PPDK_AMP/ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01418">
        <property type="entry name" value="PEP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005057">
        <property type="entry name" value="PRK06464.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43030">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43030:SF1">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00391">
        <property type="entry name" value="PEP-utilizers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02896">
        <property type="entry name" value="PEP-utilizers_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01326">
        <property type="entry name" value="PPDK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000854">
        <property type="entry name" value="PEP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01736">
        <property type="entry name" value="PHPHTRNFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52009">
        <property type="entry name" value="Phosphohistidine domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00742">
        <property type="entry name" value="PEP_ENZYMES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00370">
        <property type="entry name" value="PEP_ENZYMES_PHOS_SITE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate phosphate dikinase AMP/ATP-binding" evidence="14">
        <location>
            <begin position="19"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilising enzyme mobile" evidence="13">
        <location>
            <begin position="383"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilising enzyme C-terminal" evidence="15">
        <location>
            <begin position="477"/>
            <end position="769"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ASJ06796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="783" mass="86613" checksum="223CC530B96F94CB" modified="2017-09-27" version="1">MSEYKFIRWFEELGKNDVPLVGGKGANLGEMTNAGIPVPPGFCVTAEAYKYFVENVKLEDGTVLQDWIMNIIAQTNVDDSKQLQENTAKIRQKIIELPMLPEIAKEIEDAYKKLSQRFNKDAVYVAVRSSATAEDLPEASFAGQQETYLDVYGVDDVIDKVKKCWASLWTARATFYRAKQGFDHSKVYLSAVVQKMVNSETSGVMFTANPVTNDRSEIMINAAWGLGEAVVSGSVSPDEYIVEKGTWKIKEKYIAKKEVMVVRNPETGKGTVYVKVADYLGPEYVEKQVLTDEQIIEVAKMGAKIEEHYGWPQDIEWAYDKDDGKLYIVQSRPITTLKDEVKTEEAEMTEEMKVLLKGLGASPGVGAGKVVVIFDASEIDKVKEGDVLVTTMTNPDMVPAMKRASAIVTDEGGRTCHAAIVSRELGIPAVVGTKEATKVLKDGMLVTVDGTRGVVYEGIVKSLVKKEEEEKATGGQVVVAGAPLVTATEVKVNVSMPEVAERAAATGADGVGLLRAEHMILGIGAHPVKFIREGKEEELVEKLVEGIRKVVEAFYPRRVWYRTLDAPTNEFRELPGGEDEPEERNPMLGWRGIRRGLDQPELLRAEFKAIKRLVDEGYDNIGVMLPLVSHPEQIRKAKEIALSVGLVPHKDVEWGVMIETPASALIIEDLIKEGLDFVSFGTNDLTQYTLAIDRDNERVFKLYDEKHPAVLKLIENVIKTCKKYGVETSICGQAGSDPKMVKLLVRLGIDSVSANPDAVELVRKTVAREEARIRLEAARKALE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Q9</accession>
    <name>A0A218P7Q9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Exosome complex component Csl4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">csl4</name>
        <name evidence="5" type="ORF">A3L08_05580</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Non-catalytic component of the exosome, which is a complex involved in RNA degradation. Increases the RNA binding and the efficiency of RNA degradation. Helpful for the interaction of the exosome with A-poor RNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the archaeal exosome complex. Forms a trimer of Rrp4 and/or Csl4 subunits. The trimer associates with an hexameric ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers. Interacts with DnaG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CSL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854076.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Q9"/>
    <dbReference type="GeneID" id="33315720"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05580"/>
    <dbReference type="OrthoDB" id="6768at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00975">
        <property type="entry name" value="Exosome_Csl4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039771">
        <property type="entry name" value="Csl4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019495">
        <property type="entry name" value="EXOSC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025721">
        <property type="entry name" value="Exosome_cplx_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030850">
        <property type="entry name" value="Exosome_Csl4_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF034126">
        <property type="entry name" value="PRK09521.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12686">
        <property type="entry name" value="3'-5' EXORIBONUCLEASE CSL4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12686:SF8">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT CSL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14382">
        <property type="entry name" value="ECR1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10447">
        <property type="entry name" value="EXOSC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110324">
        <property type="entry name" value="Ribosomal L27 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0271">Exosome</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="S1 motif" evidence="4">
        <location>
            <begin position="70"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="196" mass="21544" checksum="03E0B0880169FA46" modified="2017-09-27" version="1">MDGKKGVKTGDLVLPGDYLGVIEEYFPGDGVKEENGELYAVRAGKVVIDPDRMEISVEPVTDTPPLPKVGDVVIARVIEVKPQAAIVQIVKIEGRNNDREIATSKLAGIHISQARNGYVDNLANEFRIGDIVRARVIATEKSPIQLSTKDPDLGVIYALCTRCRTPLVRRGDKLICPRCGHVETRKLSSLYRKVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7R8</accession>
    <name>A0A218P7R8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">CoA-disulfide reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_05385</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854041.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7R8"/>
    <dbReference type="GeneID" id="33315681"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05385"/>
    <dbReference type="OrthoDB" id="27922at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050451">
        <property type="term" value="F:CoA-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017758">
        <property type="entry name" value="CoA_disulphide_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050260">
        <property type="entry name" value="FAD-bd_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03385">
        <property type="entry name" value="CoA_CoA_reduc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429:SF1">
        <property type="entry name" value="NAD(P)H SULFUR OXIDOREDUCTASE (COA-DEPENDENT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="9">
        <location>
            <begin position="5"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="8">
        <location>
            <begin position="327"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="442" mass="48628" checksum="EB7EF1EB4324A3DA" modified="2017-09-27" version="1">MERKTVVIIGGGAAGMSTASRVKRLRPEWDVKVFEATEWVSHAPCGVPYVVEGLSPKEKLMHYPPEVFIKKRGIDLHMKAEVIEVEQGKVRVREPDGEHTYEWDYLVFANGASPRVPAIEGIDLPGIFTADLPPDAVAITEYMEKHDVKDVVVIGTGYIALEMAEAFVARGKNVTLIGRSERVLRKTFDKEITDIVEETLKANLNLRLGELTMRFEGDGRVEKVITDAGEYKADLVIVATGIKPNTELARELGVRIGETGAIWTNEKMQTSVENVYAAGDVAETRHLITGRRVWIPLAPAGNKMGYVAGSNIAGKEIHFPGVLGTSVTKFLDLEIGKTGLTEVEAIKEGYDVRTAFIKAKTKPHYYPGAREIWLKGVVDNETNRLLGVQAVGAEILPRIDTAAAMLTAGFTTKDVFFTDLAYAPPFAPVWDPLIVLARVLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7S3</accession>
    <name>A0A218P7S3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">KaiC-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_232461675.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7S3"/>
    <dbReference type="GeneID" id="33315718"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05570"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="24900" checksum="010039B1D5E6282F" modified="2017-09-27" version="1">MFLTRAIEKGYFTVVSNYSVPLRSFVRHGVSVGLDVEKALSGEKMAIIDVFDSGYAKLKDSLPGVFYLDSVEPETINPKLDRIYCEELEEKLRSGKALRVIYTLDGAALMLGEENTLRLLNQTVAAKSVGMPNSILLLPVNADIVSKRFVAWISNISDYVLLAKSWIREDHIKEILYLIKAPNPDFEPAVYSMKIKKGANRIRLKKLRSAPELRPPAEEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7S6</accession>
    <name>A0A218P7S6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tritrans,polycis-undecaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific)</fullName>
            <ecNumber evidence="4">2.5.1.89</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Undecaprenyl diphosphate synthase</fullName>
            <shortName evidence="4">UDS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Undecaprenyl pyrophosphate synthase</fullName>
            <shortName evidence="4">UPP synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">uppS</name>
        <name evidence="5" type="ORF">A3L08_05560</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the sequential condensation of isopentenyl diphosphate (IPP) with geranylgeranyl diphosphate (GGPP) to yield (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30E,34E,38E)-undecaprenyl diphosphate (tritrans,heptacis-UPP). It is probably the precursor of glycosyl carrier lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>geranylgeranyl diphosphate + 7 isopentenyl diphosphate = tri-trans,hepta-cis-undecaprenyl diphosphate + 7 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:27622"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:60388"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="2.5.1.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="4">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the UPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.89" evidence="4"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854073.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7S6"/>
    <dbReference type="GeneID" id="33315716"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05560"/>
    <dbReference type="OrthoDB" id="8293at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016094">
        <property type="term" value="P:polyprenol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1180.10:FF:000003">
        <property type="entry name" value="Isoprenyl transferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291:SF43">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT DHDDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="4">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="44"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="88"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="219"/>
            <end position="221"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="264" mass="31371" checksum="18358246384792CC" modified="2017-09-27" version="1">MLRGLLSHIPHIIFKPVYDIYEGYLLEKVKSRELPSHVAIIMDGNRRWARKLEKPPWYGHLFGSKKLEEILEWCHELGIRTLTVYAFSTENFKRTPEEVNALMNLFEQKFKELIQDERVHKYGIRVNVLGRKELLPENVRKAAEEAERVTRKYTNYTLNVALAYGGRSEIADAVKDIVRDVQEGRISVEDIDEGLIKRYLYYPNMSDPDIVIRTGGEVRISNFLLYQIAYSELFFVDVYFPEFRKIDFLRIIREYQKRQRRFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7U2</accession>
    <name>A0A218P7U2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ferritin-like diiron domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7U2"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05740"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01045">
        <property type="entry name" value="Ferritin_like_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rr_diiron-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33531:SF10">
        <property type="entry name" value="BLR7895 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33531">
        <property type="entry name" value="RUBRERYTHRIN SUBFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="1">
        <location>
            <begin position="19"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="175" mass="20284" checksum="36757BC0ECDCFB31" modified="2017-09-27" version="1">MELKFSGEEKRNLQSIIDKLKRLSPQELLSYWINAELDEAETYNTLAEIVREYSWDPKIPKLFEELARESLEHAELLLKEYRRTYGGEKLIDTGIPGIELELSMGELERYIRSGRLGDLISVLMESERMAQEIYRCLAEKSSGKTRELFEHLASIENGHYLRLKALKESLEESGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7U5</accession>
    <name>A0A218P7U5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA repair protein Rad25</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_05660</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854092.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7U5"/>
    <dbReference type="GeneID" id="33315736"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05660"/>
    <dbReference type="OrthoDB" id="11644at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050615">
        <property type="entry name" value="ATP-dep_DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049430">
        <property type="entry name" value="UvsW_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274">
        <property type="entry name" value="RAD25/XP-B DNA REPAIR HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="103"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="303"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="464" mass="53477" checksum="D4BA9E3AB70DA65C" modified="2017-09-27" version="1">MVILRIPDGSALVRIEKADPKVYFSIYELLSYKKDYGKWEKPESLYDPYEKTFPVGLLPRVKKFLNSRGYRVRVKDERQVRGVKLNSTWNENYVMRKYQQRAVKKALKEKMGVLALPVGSGKTVVGLRIIHELDLSALIVVHTKELLYQWADKVREVLGIEPGIVGDNKWNEGSVTIAMVQTLLSRGADKLQHDYAIVMFDECHRTSAAEKFYQLGLSLPQVYRFGLSATPWRRVRGEEIKIEAVVGPTIFEVKAEDLIKEKFLAKPRFEVITYESAMPSFSERYKELYEDMIMNNDERNLAVVEKAVELARKGHRVLIDVKRIEHGKILKEMLEKRGMKAEFLSSKSPNRWEILEAFKEGEIPVLISTLLKEGVDIPEISAIILAGGGKSDIMTIQTIGRALRPKRGMRAVIVDVQDDDPLLFTHFIERQKALKQYYGKYYDKEMASKLEESVPKKRRPRKRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Y0</accession>
    <name>A0A218P7Y0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854145.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Y0"/>
    <dbReference type="GeneID" id="33315790"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05930"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="71" mass="8042" checksum="93FE9CD78C2A54DD" modified="2017-09-27" version="1">MKGGEIKGALAFVGILILGLLFFFGFLVIALALILAGLLLFLGFYAYLRLKLWWVRKHPPKELEGPEDYLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Y1</accession>
    <name>A0A218P7Y1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sodium:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_05865</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854132.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Y1"/>
    <dbReference type="GeneID" id="33315777"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05865"/>
    <dbReference type="OrthoDB" id="76879at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018461">
        <property type="entry name" value="Na/H_Antiport_NhaC-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43478">
        <property type="entry name" value="NA+/H+ ANTIPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43478:SF1">
        <property type="entry name" value="NA+_H+ ANTIPORTER NHAC-LIKE C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03553">
        <property type="entry name" value="Na_H_antiporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="344"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="385"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="478"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="503"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="Na+/H+ antiporter NhaC-like C-terminal" evidence="7">
        <location>
            <begin position="167"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="564" mass="60465" checksum="7AC1D3F20D232A0B" modified="2017-09-27" version="1">MTDYGILSLLPPLVAIGLAIVTKRVLLALFSGVWVGGLLVAGGNPIEATTETLKWIVLNIAAAWEEDGHLVTDLWNTRILVFDALIGAGVALIYKAGGMNAIAKAVTRKIRTSRAASLMAAVFGTLIFFDDYTNTIIVGNTMRPITDRARVSREFLAYADDSTAAPVAVLAVVSTWIGYELGLLKDAIASVGESISAYSAWFASWPYRLYPILAVLLVYIVAVTGRHYGPMLRAEYRARTTGKVLRDGAQPMMTTEVDVGMPIEGKENVWVFVLPVLTLILMTFLGLWVTGGGSATYAQGGFQEVLSHADSTWALVWGSFSMLVVAMALILGRRIMSLEEVESTIVSGMKQMHFAMMILILAWSIKRACDAVGTADYIVRVASNVLSPSLVPLVVFVVAAFISFTTGTSWGTFAIMMPIAVPLAYNLSGEFGPVVYASIASVFSGGVLGDHCSPISDTTIMSSMFSGCDHLDHVNTQIPYALTAGVVSVIMLLLFAAGLQNGWLLLAMAVPLLVVFHRLLSEWYGARIGIPGGRVPVYVVGDDYGETLGEETESEGGALTYAVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P821</accession>
    <name>A0A218P821_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854171.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P821"/>
    <dbReference type="GeneID" id="33315820"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06085"/>
    <dbReference type="OrthoDB" id="92696at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd18704">
        <property type="entry name" value="PIN_VapC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13638">
        <property type="entry name" value="PIN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="109"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="332" mass="38787" checksum="9EBAFA4432FF7993" modified="2017-09-27" version="1">MWGTSEVIEKPELQILLNLLPEIRVSYPLYGLQLLRAKPIETGYRVSVTVNRHEFNERVPERLSNELPTYTDFYECFISSGIIRYENVDEFAQNLELYERLKKGVVFAPDTNLFYHRFISSFRPLNGYQIVVAEGVKKEIEDAMNYKYRNKQLDDMRREVRNAALLREFSNRRTKKSRKAAYIALKEFEALKSRIIIAESVNEPAHNNDEIIVKSLKRYDRMTPTLLVFLTADIAVTDVAEMEGLEYFLFKYPREKLGRHDVSAYQLRTLLFNLAAVFGVIEVNGILIFGEFRGKQGLNELKVVFPAESRTYHEFEFHLKLSRKLMEIMGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P827</accession>
    <name>A0A218P827_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Metal-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854174.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P827"/>
    <dbReference type="GeneID" id="33315823"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06100"/>
    <dbReference type="OrthoDB" id="73362at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019271">
        <property type="entry name" value="DUF2284_metal-binding"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10050">
        <property type="entry name" value="DUF2284"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018748">
        <property type="entry name" value="UCP018748"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="153" mass="17792" checksum="5FF13D650300C875" modified="2017-09-27" version="1">MRVLWEREIDASEIVVSPRPVWKCRACPMYGKRPSCPPYAPNWREAKDWVGHFRRAFLIKFEIDMENFEGEKRKALLYLLKREEELFKEGHMYAMALFPGSCNLCDDCPFEHGQACRFPTTVRPSINAIGIEIGKLVKIDFSESVLYGMVLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P836</accession>
    <name>A0A218P836_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cation:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_06205</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854191.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P836"/>
    <dbReference type="GeneID" id="33315844"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06205"/>
    <dbReference type="OrthoDB" id="18006at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050601">
        <property type="entry name" value="CPA3_antiporter_subunitC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039428">
        <property type="entry name" value="NUOK/Mnh_C1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34583">
        <property type="entry name" value="ANTIPORTER SUBUNIT MNHC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34583:SF2">
        <property type="entry name" value="ANTIPORTER SUBUNIT MNHC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00420">
        <property type="entry name" value="Oxidored_q2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="114" mass="12005" checksum="FD63B0ADEADED4C0" modified="2017-09-27" version="1">MISVYYFGAIALILIGLYAVLVKKNVLKMLIGLSIMETGVNLLLVSVGYISGRSAPILSEGIGPNGAVDPIPQALVLTAIVIGVATTAMALSVAILIHEKYGTLNVEEIRRLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P838</accession>
    <name>A0A218P838_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cation:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_06210</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854192.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P838"/>
    <dbReference type="GeneID" id="33315845"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06210"/>
    <dbReference type="OrthoDB" id="99605at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050622">
        <property type="entry name" value="CPA3_antiporter_subunitB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007182">
        <property type="entry name" value="MnhB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046806">
        <property type="entry name" value="MrpA_C/MbhE"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006248">
        <property type="entry name" value="PRK08386.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009217">
        <property type="entry name" value="PRK12567.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33932">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33932:SF4">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20501">
        <property type="entry name" value="MbhE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04039">
        <property type="entry name" value="MnhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="MrpA C-terminal/MbhE" evidence="8">
        <location>
            <begin position="33"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Na+/H+ antiporter MnhB subunit-related protein" evidence="7">
        <location>
            <begin position="98"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="235" mass="25623" checksum="C42FA6779B997EBE" modified="2017-09-27" version="1">MLKRALAIISLLIIGYWLAQGLAGVPFGQDRMLVGQYYLDHVKEQTGAVNAVTAVVVNYRGFDTLGEVTVLFIASTGVGALLWRKKRERTAKTEGSIVLTTGTRLLAPFIMLFGAYIFIHGHLTPGGGFPGGATIATAFLLLYMAFTVYEIPHKAFEKVEGAVGMSYVLVGLIGLAIGGYFLFDWIWQTWGWGHNNIGRLFSGGFIPVIYTVIGIKVGTELSGIVDNMLKEEVRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P873</accession>
    <name>A0A218P873_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TIGR00297 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_06405</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854228.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P873"/>
    <dbReference type="GeneID" id="33315884"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06405"/>
    <dbReference type="OrthoDB" id="28948at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002794">
        <property type="entry name" value="DUF92_TMEM19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13353">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13353:SF5">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01940">
        <property type="entry name" value="DUF92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="24734" checksum="DCF326372437D50E" modified="2017-09-27" version="1">MLERIALEVAVVAGLGLSAYRLRALDARGAVAAAVLGLAVIELGGVYPFLAMLVFVVAGVLATKYRFREKARLGVAQEGNGVRSWGNVLGNGLAAVIFLIFEYISQRDVFWAATFAAIATANGDTLASELGKVFGKTPRLITDLRPTRPGTNGAVSWAGELFAIIGALMIALLALPLTCYKTQMLLAVTAGGFLGANIDSIIGATLENRGVTDNNSTNFLASLLGGIIGAGLFYLIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P881</accession>
    <name>A0A218P881_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">pH regulation protein F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_06545</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854255.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P881"/>
    <dbReference type="GeneID" id="33315912"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06545"/>
    <dbReference type="OrthoDB" id="84883at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015075">
        <property type="term" value="F:monoatomic ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007208">
        <property type="entry name" value="MrpF/PhaF-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04066">
        <property type="entry name" value="MrpF_PhaF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="89" mass="9646" checksum="06F3EEC77ECA67CF" modified="2017-09-27" version="1">MFENTILLLMVLYGVAGVIYFIRVLLGPTVLDSILAGDCVSLDVALIALLIAVYYENNLLAGGAFFLVLWAFVLDIFASKYLVKGEVGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P891</accession>
    <name>A0A218P891_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Protein frxA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06455</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854238.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P891"/>
    <dbReference type="GeneID" id="33315894"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06455"/>
    <dbReference type="OrthoDB" id="85598at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00518">
        <property type="entry name" value="H2MP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1450">
        <property type="entry name" value="HybD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023430">
        <property type="entry name" value="Pept_HybD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000671">
        <property type="entry name" value="Peptidase_A31"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00072">
        <property type="entry name" value="hydrog_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30302:SF7">
        <property type="entry name" value="F420-NONREDUCING HYDROGENASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30302">
        <property type="entry name" value="HYDROGENASE 1 MATURATION PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01750">
        <property type="entry name" value="HycI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00446">
        <property type="entry name" value="HYDRGNUPTAKE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53163">
        <property type="entry name" value="HybD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="149" mass="16457" checksum="7BA2D896C55F2505" modified="2017-09-27" version="1">MRTLILALGNELMRDDGVGLKAGRILAEKGYNVLEVGTDIFRLASHYHGEDRIIIIDAILSDKLKPGEIIHLQGDDVCEKLKAEIRSAHFMGAIDGLKLLMALDERLAKVDIHFIGVVAKEIDLGMELSDEVENALPRVVELVESLCKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8B0</accession>
    <name>A0A218P8B0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Hydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_06535</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I 20 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854253.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8B0"/>
    <dbReference type="GeneID" id="33315910"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06535"/>
    <dbReference type="OrthoDB" id="92751at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052375">
        <property type="entry name" value="Complex_I_20kDa-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42989:SF1">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42989">
        <property type="entry name" value="HYDROGENASE-4 COMPONENT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56770">
        <property type="entry name" value="HydA/Nqo6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase-like 20kDa subunit" evidence="7">
        <location>
            <begin position="14"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="253" mass="28951" checksum="3AF65E4318118B2C" modified="2017-09-27" version="1">MKSLWVFHVNTGACNGCDIEILDVLTPYYDVERLGVKLVPTPRHAHALLVTGPLTRQAYHAARMAYEAMPPKPRLVIAVGTCACSGGIFHDSYALRREHRESFEYPLRGGASEFLPVHLYIPGCPPRPEEILYGIALLKNIVEKKVRGSWFSESAFVLPRESFNAWVEVLLRARIRKELGYFDGYPLLRRFMELVESSESKEELEKLVQKAISEESDSRLRYGLEKLYSYYLEVVRTYESILAAKRALVRLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8D0</accession>
    <name>A0A218P8D0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2-oxoglutarate ferredoxin oxidoreductase subunit gamma</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_06770</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854293.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8D0"/>
    <dbReference type="GeneID" id="33315957"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06770"/>
    <dbReference type="OrthoDB" id="18183at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016903">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052554">
        <property type="entry name" value="2-oxoglutarate_synth_KorC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006211">
        <property type="entry name" value="PRK08338.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42730">
        <property type="entry name" value="2-OXOGLUTARATE SYNTHASE SUBUNIT KORC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42730:SF1">
        <property type="entry name" value="2-OXOGLUTARATE SYNTHASE SUBUNIT KORC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate/ketoisovalerate oxidoreductase catalytic" evidence="2">
        <location>
            <begin position="9"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="170" mass="18155" checksum="77345A835745916A" modified="2017-09-27" version="1">MQIRFAGIGGQGVVLAGVILGEAAAIEGLNVVQTQDYSSASRGGHSIADVIISKEPIYDVIVTKADVLVALAQLGYDTVKDELKEGGLLIIDTDLVKPDRDYIGAPFTRLAEETTGLALTVNMVALGYLVAKTGVVKKENVEEAIRRRVPKGTEEINIRAFRAGFEEGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8D4</accession>
    <name>A0A218P8D4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06810</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854301.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8D4"/>
    <dbReference type="GeneID" id="33315965"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06810"/>
    <dbReference type="OrthoDB" id="94128at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="150" mass="16805" checksum="8A516B96E3D4790E" modified="2017-09-27" version="1">MKLRSLLYLLIFPAWEFLLMILLPPEYYVGASCLQTHPYPICSFHFSPSATFVLLVIAPFLISIILGLWKEWSELVAFGVPSLALVLIAVILLYLFDGSLISFLVPATVSVVLYSLPRIKRAERPIWIATSLIVLFILCTMVWSGTSVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8F8</accession>
    <name>A0A218P8F8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF2226 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854270.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8F8"/>
    <dbReference type="GeneID" id="33315929"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06630"/>
    <dbReference type="OrthoDB" id="86210at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="571" mass="63483" checksum="89446848263210C1" modified="2017-09-27" version="1">MQLPDKSPLLENMVVTSAEELAGLIQRALSQGNGAFLKIFTKDAKGKYYVTVLLDRSKVLAAECLVLDTKQNLSGEEAIRMLKSFIGKPMVVDVYPLDELELKLSVADNVDVYAQTPKTPLEELLKAHEGGPPSGEKKPVEEKEPELPKKEPPSATSTSTTPTPRPPAVVETPAEKPKPAPFGKPEVIIKMSGGKMPEKAFHVYAEDLLKEAKRIRGLTINRIEFDANVGDGVVYLNVHIYGNSTGGSRDIEIAEKRMLHAVSKYAPVLLREAEIKPIIRDVSVIIDGQEVRPQEIVDKDKKKTANVTKDGKIVLSVLEDVWPYFSAFARTVVAEIESAGIKVDKAHFDIKGRREFEINLSMVAEAKMAEDEVQRVVRDVVGRHARELGRTLNRYITVHNVEVKTITKTAPSTTTRISDGVEASSKAAEILAKKELLEKEVEQLLKQAGIDELSTLTEEKKKESEETVLKSRIEPAIETLKNRVHAELKLVPRVTFKWLKLNHEVKGSTVYVDIEASFLRENVGGLFGSFSGISDEKIKRDITSTIQRVIKDVSREYGVNMNLKRLSVILR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8J4</accession>
    <name>A0A218P8J4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein A3L08_07135</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_07135</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CinA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854361.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8J4"/>
    <dbReference type="GeneID" id="33316030"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07135"/>
    <dbReference type="OrthoDB" id="372037at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00885">
        <property type="entry name" value="cinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00226_A">
        <property type="entry name" value="CinA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050101">
        <property type="entry name" value="CinA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023055">
        <property type="entry name" value="CinA_Arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002977">
        <property type="entry name" value="PRK03670.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13939">
        <property type="entry name" value="NICOTINAMIDE-NUCLEOTIDE AMIDOHYDROLASE PNCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13939:SF0">
        <property type="entry name" value="NMN AMIDOHYDROLASE-LIKE PROTEIN YFAY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="2">
        <location>
            <begin position="4"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="252" mass="28619" checksum="35E6AC9104398586" modified="2017-09-27" version="1">MFAEILTVGDELLTGNTVDSNSTFIAQRLTERGYWVRRKTTVGDDVEEIKTVIREILDRKPEVLVISGGLGPTHDDVTMLAVAEALGKRLVLCEECLEKIRTFYERLHREGYIDDPELNEGRRKMAYLPKGAEPLENTEGAAPGAYIEHDGVKIFVLPGMPREMKAMLENEVLPRLGRRKFIQRKLLAEITDESKLAPLLIETLERFNVRIHSSPKGFGKYIGIIIFGENEEEIERAKAFMEGKGVRFEEGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8L8</accession>
    <name>A0A218P8L8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hydrogenase assembly protein HupF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_07225</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HypE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854378.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8L8"/>
    <dbReference type="GeneID" id="33316048"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07225"/>
    <dbReference type="OrthoDB" id="31494at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06061">
        <property type="entry name" value="PurM-like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011854">
        <property type="entry name" value="HypE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30303:SF4">
        <property type="entry name" value="HYDROGENASE EXPRESSION_FORMATION PROTEIN HYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30303">
        <property type="entry name" value="HYDROGENASE ISOENZYMES FORMATION PROTEIN HYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005644">
        <property type="entry name" value="Hdrgns_mtr_HypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="2">
        <location>
            <begin position="38"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="3">
        <location>
            <begin position="155"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="332" mass="36900" checksum="88A5FFE39D9F1ED0" modified="2017-09-27" version="1">MKELLPGKLRNEVLREIVFQNLGVEDTAVVHGPREGFDSAVLEYDAGHYLIVATDPTLGVPRETFGFFTYHFASSDVAVFGARPRWLVVDLLFPSGTGEDFLEAVMRDINNECRRYGSTVIGGHTGAYPSVSKPTATTTAMGLVKKEELKIPVARPGDKIVVTAKVGLEFAVSAAYFREDELRKFLSFREIERLKKLYRFETVVPDALTARPFVRGMHDATEGGLTALHEIAGNSGVGFRVYADRLWIDLLVRKVLDFYGLEPWSVSSSGTLIAITPPENVGSLIAELNKNGIMAFELGEFTEEKERILVENGEERPFPEFEGDPYVRLYGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8N8</accession>
    <name>A0A218P8N8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_07290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854390.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8N8"/>
    <dbReference type="GeneID" id="33316061"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07290"/>
    <dbReference type="OrthoDB" id="101251at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053615">
        <property type="entry name" value="PCNA-interact_regulator"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041142">
        <property type="entry name" value="PCNA_Inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="64" mass="7796" checksum="9D63236995DF671E" modified="2017-09-27" version="1">MDKTLDEFLRSTVRKVKPEEEPRKRKKRLKPTSLESFLPEEHVNYFKQLRIGSKRIRNARIEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8R5</accession>
    <name>A0A218P8R5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dolichol-phosphate mannose synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07515</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854428.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8R5"/>
    <dbReference type="GeneID" id="33316106"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07515"/>
    <dbReference type="OrthoDB" id="11098at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04179">
        <property type="entry name" value="DPM_DPG-synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859:SF105">
        <property type="entry name" value="DOLICHYL-PHOSPHATE BETA-D-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859">
        <property type="entry name" value="GLYCOSYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="1">
        <location>
            <begin position="8"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="222" mass="25334" checksum="149E22C2A7E8721E" modified="2017-09-27" version="1">MLKGKRISVIIPAYNEERRLPLVLEKIPDFVDEVIVVDDGSSDKTYEVARVFAEKDPRINVIGLEKNCGKGCAMREGVKSSSGDVVVFMDADGQHKPEDIIKLVEPIANGEADMVIGARKVEEAGKRPLHRRLSNIITTRLIRLKLRTYIYDTQSGFRAYRRDFLPEIESDRYEVETEMLLKAAKMGARIKEVPVSMIYDPAREGRFGPRDVIRFIKTYLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8T5</accession>
    <name>A0A218P8T5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 16">tRNA-splicing ligase RtcB</fullName>
            <ecNumber evidence="16">6.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">rtcB</name>
        <name evidence="18" type="ORF">A3L08_07550</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Essential for tRNA splicing and maturation. Acts by directly joining spliced tRNA halves to mature-sized tRNAs. Joins RNA with 2',3'-cyclic-phosphate or 3'-phosphate ends to RNA with 5'-hydroxy ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP + H2O = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68080"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:68076"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="15 16">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 16">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 16">Belongs to the RtcB family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.-" evidence="16"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854434.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8T5"/>
    <dbReference type="GeneID" id="33316113"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07550"/>
    <dbReference type="OrthoDB" id="9887at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003972">
        <property type="term" value="F:RNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0170057">
        <property type="term" value="F:RNA ligase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1860.10:FF:000007">
        <property type="entry name" value="tRNA-splicing ligase RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1860.10">
        <property type="entry name" value="tRNA-splicing ligase RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001233">
        <property type="entry name" value="RtcB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036025">
        <property type="entry name" value="RtcB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118">
        <property type="entry name" value="RNA-SPLICING LIGASE RTCB HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118:SF1">
        <property type="entry name" value="RNA-SPLICING LIGASE RTCB HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01139">
        <property type="entry name" value="RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103365">
        <property type="entry name" value="Hypothetical protein PH1602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 16 18" id="KW-0436">Ligase</keyword>
    <keyword evidence="8 15 16" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="active site" description="GMP-histidine intermediate" evidence="13">
        <location>
            <position position="402"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="201"/>
            <end position="205"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="327"/>
            <end position="328"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="376"/>
            <end position="379"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="383"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="402"/>
            <end position="405"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="478"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU371113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ASJ07183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="479" mass="53201" checksum="B0D4E33E413DAED6" modified="2017-09-27" version="1">MVPLKRIDKIRWEIPKFDKRMRVPGRVYADDALIEKMRQDRTLEQAANVAMLPGIYKYSIVMPDGHQGYGFPIGGVAAFDVKEGVISPGGVGYDINCGVRLIRTNLREQEVRPRIKELVDTLFKNVPSGLGSKGRVRLHWTQLDDVLADGAKWAVENGYGWEEDLEHLEEGGRMEGADPEAVSQKAKQRGAPQLGSLGSGNHFLEVQVVDKIFNEEIAKAYGLFEGQVVVMVHTGSRGLGHQVASDYLRVMEKANRKYGIPWPDRELVSVPFQSEEGQRYFSAMKAAANFAWANRQMITHWVRESFEEVFKRKAEDMEMHIVYDVAHNIAKVEEHEVDGKKVKVVVHRKGATRAFPAGHPDVPRAYRDVGQPVLIPGSMGTASYVLAGAEGSMRETFGSSCHGAGRLLSRKAATRQYRGDRLRNELAQRGIYVRAASLRVVAEEAPGAYKSVDNVVNTVHEAGIANLVARMRPMGVAKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8V4</accession>
    <name>A0A218P8V4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carbohydrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854469.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8V4"/>
    <dbReference type="GeneID" id="33316154"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07760"/>
    <dbReference type="OrthoDB" id="23670at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02208">
        <property type="entry name" value="cupin_RmlC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052538">
        <property type="entry name" value="Flavonoid_dioxygenase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43346">
        <property type="entry name" value="LIGAND BINDING DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G14370)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43346:SF1">
        <property type="entry name" value="QUERCETIN 2,3-DIOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-2" evidence="1">
        <location>
            <begin position="32"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="114" mass="13392" checksum="F0EC562E7DA9C194" modified="2017-09-27" version="1">MKAEIKNLIDRGTYRKLPLFEGELPEGSYAQIVEVKPNQTVKKHYHERQYELFYIISGEARLGIGETEYLAKPGDIFLVKPKTVHWVVNEKDEPFRLFVVKLNYYGDDSVWLDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P904</accession>
    <name>A0A218P904_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Iron ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_07985</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854512.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P904"/>
    <dbReference type="GeneID" id="33316202"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07985"/>
    <dbReference type="OrthoDB" id="27848at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033214">
        <property type="term" value="P:siderophore-iron import into cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06550">
        <property type="entry name" value="TM_ABC_iron-siderophores_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3470.10:FF:000001">
        <property type="entry name" value="Vitamin B12 ABC transporter permease BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="entry name" value="ABC transporter involved in vitamin B12 uptake, BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472:SF25">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE PROTEIN MJ0876-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="FERRIC ENTEROBACTIN TRANSPORT SYSTEM PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="ABC transporter involved in vitamin B12 uptake, BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="253"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="312"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="344" mass="36680" checksum="0B299DBAB7882E45" modified="2017-09-27" version="1">MDYERYVARKLSIGVLLFLFTILVSLYSLSHGSYSIPLREVLDALLGKGNESAHLVVWKVRMPRIVAGLLVGSALAVAGAVMQGFLRNPLATPFTMGVSHGAMFGASLAIILGAGYAESSGRISLDNPYAVVLFAFIGAMTAVGVILLLARLKGLSPEAIILAGVAMSSLFVALTTFVQYFADELQLAAMVYWSFGDLGRATWREDAIMLAVFTPVFIYFVIKRWDLNASAAGDEVAKSVGVEVERVRLISTFLAALITAVSVAFVGVIGFIGLIAPHAIRLVAGGDYRFLIPLSALAGAFLLVTADTVARLVLAPMVLPVGIVTSFLGAPTFIYLLVKMEGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P916</accession>
    <name>A0A218P916_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Gins51 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_07955</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854506.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P916"/>
    <dbReference type="GeneID" id="33316196"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07955"/>
    <dbReference type="OrthoDB" id="86040at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd11581">
        <property type="entry name" value="GINS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21695">
        <property type="entry name" value="GINS_B_archaea_Gins51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054314">
        <property type="entry name" value="Gins51_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22090">
        <property type="entry name" value="Gins51_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gins51 C-terminal" evidence="2">
        <location>
            <begin position="142"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="121"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="193" mass="22048" checksum="7D5059B4585C41D4" modified="2017-09-27" version="1">MDIVKLRELLEQELSSPDLVDLDGEFYKEFDSLIKALKLSAESSRERGEDVEERLYLAQLEIAEKLAREIIKIRLHKIVDLVVEGIPAEMTEEERKIFTILRAFIEREELNVPLSGGELAETVPESTPEQPSEPSKKSIPTEAYIIKVDLPRVLDPELREYGPFRAGDMVILPRSIAKVLVERDVAERIRLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P976</accession>
    <name>A0A218P976_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rubrerythrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854583.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P976"/>
    <dbReference type="GeneID" id="33316282"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08380"/>
    <dbReference type="OrthoDB" id="103571at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01045">
        <property type="entry name" value="Ferritin_like_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rr_diiron-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33531:SF10">
        <property type="entry name" value="BLR7895 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33531">
        <property type="entry name" value="RUBRERYTHRIN SUBFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rubrerythrin diiron-binding" evidence="1">
        <location>
            <begin position="7"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="174" mass="20651" checksum="2160DF3D6AC4D1A7" modified="2017-09-27" version="1">MEITDKEVFEIARNAEIRAKEAYEKLASAVKSDIIRDELLFLAREEDKHREIIEKMAERFQSGEGEPKKVEIDVMAEFKIIAEKMAEAIKKPDLNLDEVYEIAMEAELVSEKLYNELAKYAATEKTKVLLNMLADMERNHYNILKKQYDYITRYPEIYKEEFYDQLMKDINFNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P979</accession>
    <name>A0A218P979_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Anion transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_08440</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arsA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854594.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P979"/>
    <dbReference type="GeneID" id="33316294"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08440"/>
    <dbReference type="OrthoDB" id="46198at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02035">
        <property type="entry name" value="ArsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025723">
        <property type="entry name" value="ArsA/GET3_ATPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016300">
        <property type="entry name" value="ATPase_ArsA/GET3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00345">
        <property type="entry name" value="GET3_arsA_TRC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803">
        <property type="entry name" value="ARSENICAL PUMP-DRIVING ATPASE ARSENITE-TRANSLOCATING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803:SF3">
        <property type="entry name" value="ATPASE GET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02374">
        <property type="entry name" value="ArsA_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ArsA/GET3 Anion-transporting ATPase-like" evidence="2">
        <location>
            <begin position="12"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="330" mass="37632" checksum="CFF34F06C66C17EA" modified="2017-09-27" version="1">MREYLLPKKDYRVLFVIGKGGVGKTTTSASIAVALAEKRYKTLIVSLDPAHNLGDVFMTKLGDRPKKLAENLYASELDMEKLIKGYLEHLEKNLKHTYRYLTVINLDKYFEVLRYSPGIEEYATLEAIRDILMKGDEWDVIVFDTPPTGLTLRVLALPKISLIWADKLIDVRRKILEKRAAIANIQGPLKFKAGEEEAELPTEESRDEVMNELKKYRSEVQFVDGVITDPEKTSVVAVMNPEALPLYETIRARDTLKKFRVPFRLIVVNKVINVSGEIPELKVKLDAQKKVLEQIKGEFRGVEVLKIPMFAEEPRGMEWLKRLGGMIVGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P983</accession>
    <name>A0A218P983_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Alanyl-tRNA editing protein AlaX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_08395</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854586.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P983"/>
    <dbReference type="GeneID" id="33316285"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08395"/>
    <dbReference type="OrthoDB" id="11392at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.130:FF:000032">
        <property type="entry name" value="Alanyl-tRNA editing protein AlaX-M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051335">
        <property type="entry name" value="Alanyl-tRNA_Editing_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43462">
        <property type="entry name" value="ALANYL-TRNA EDITING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43462:SF1">
        <property type="entry name" value="ALANYL-TRNA EDITING PROTEIN AARSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alanyl-transfer RNA synthetases family profile" evidence="5">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="212" mass="24851" checksum="96D5BC1D312941BB" modified="2017-09-27" version="1">MTRKLYYEDAYLKEAKAKVLEVRENALLLDQTIFYPTGGGQPHDKGTINGVEVLDVYKDDAGNVWHVVAEPERFKVGDEVELKIDWDYRYRLMRIHSAMHLLEHVLNEVLPGEWELYGSGMSAEKGRYDIVYPENVNQYKEQIIETFNRYVDEGGEMKIWWEGETRYTQIRDFEVIPCGGTHVRDIKEIGHLKKLKRSSLGKGKQRIEIWLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P993</accession>
    <name>A0A218P993_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P993"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08500"/>
    <dbReference type="OrthoDB" id="1018at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050508">
        <property type="entry name" value="Methyltransf_Superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42912">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42912:SF80">
        <property type="entry name" value="METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="44"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="209" mass="23933" checksum="B6EF582AB04F5024" modified="2017-09-27" version="1">MPKVEPFEKHRDRYENWFERHRYAYLSELEAVRTLLPKEGKGAEIGVGTGRFAAPLGIKLGVEPSKAMAEIARKRGIEVIEGVAENLPFTDESLDYLLMVTTICFVDDPEKALREAYRVLKPSGALIIGFVDRNSLIGRYYEEHRGESVFYRDARFFSTEELLELLKKVGFRKFEIVQTLFHRLDEIKEVEPAKPGYGEGSFVVIKAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9B6</accession>
    <name>A0A218P9B6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MFS transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_08625</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9B6"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08625"/>
    <dbReference type="OrthoDB" id="86286at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23518">
        <property type="entry name" value="C-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23518:SF2">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="313"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="375"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="400"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="1"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="422" mass="46357" checksum="8A82F73BF18A4DD2" modified="2017-09-27" version="1">MKGRPQKRKRTTLKTLEKGRQMKHRPDPGKWFYSFIPFKVSTGGAAPLIPLLTMNLGGGPTDVGAVNAIGSTASMLGGLFWGKLSDKLNRRKVFLLTGFLGTAVASILFALAKSVHQVMAVNAIYTFFIAATIPLPILIITKAFRLEDWDYAIGRFNEIGGWAWVAGMVLGLVMARFLSLRDIFVVLGLIGLLSFPWGLKTIREVPLHIDRRVLGIYTGYVVEKFRYLPNMITHLPRFSTEGFGRLYISTILFWIGAMLYFTQFPVLLKSRGLGATELYIMSIANSAVSAYMYTRVGLRLRKTGGYGALKKGLGLRGVAFLLLAVSTFVRASVFFVLAFISYVIAGYTWSYISVSTSSIISRRAPPKKKGSLIGAYNLVSSLGAIIGNLISGFVVEALGFTADFALASSLMFLALVPILRER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9E7</accession>
    <name>A0A218P9E7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_08715</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854639.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9E7"/>
    <dbReference type="GeneID" id="33316349"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08715"/>
    <dbReference type="OrthoDB" id="197151at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06262">
        <property type="entry name" value="metallo-hydrolase-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051453">
        <property type="entry name" value="MBL_Glyoxalase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46233">
        <property type="entry name" value="HYDROXYACYLGLUTATHIONE HYDROLASE GLOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46233:SF3">
        <property type="entry name" value="HYDROXYACYLGLUTATHIONE HYDROLASE GLOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="5">
        <location>
            <begin position="31"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="239" mass="27306" checksum="C28AB9854AA167EF" modified="2017-09-27" version="1">MRISQIEEFPRELVPVEVPPHTVMLRGIAWDSNVYLVRDGKETLIIDTGTGVNWHVHTGIWEREGYLNGIKKAIIFNTHEHFDHVGGNRPLREWLEGKGIEVLFAAHEVTAKTLEKGNDYVILAYSYGRKFEPQKVDLKLKDGDRLRIGSLELELVHTPGHTAGSSCLYLDDGETRLMFTGDTLFRGTVGRTDLPTGNGWELRESLEKLLEFEVDFGLPGHGRYIDDWKRNLREILGWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9I3</accession>
    <name>A0A218P9I3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09000</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_198362155.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9I3"/>
    <dbReference type="GeneID" id="33316406"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09000"/>
    <dbReference type="OrthoDB" id="85825at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="203" mass="23198" checksum="D09434E3C5CA397C" modified="2017-09-27" version="1">MKFKGKAFGSMVRMEFDILTLSELKIDDLRDFDVDSIRIELKPTSSGIKVIGIWEGTVENAGEGIKKALLESYRLRERVLNRIKSKTEAIRGTMQQLGFKEEVEGYGSVLRFIKKVGPYSIVVVASTTDDIVRVEVYGNDKKIISPELESIFEDVEVEELEVYDFEDGKEERLVINLEIPKNEEKPEKKIVEAIRMIESMLMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9J3</accession>
    <name>A0A218P9J3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Restriction endonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854691.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9J3"/>
    <dbReference type="GeneID" id="33316407"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09005"/>
    <dbReference type="OrthoDB" id="95588at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015666">
        <property type="term" value="F:restriction endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007560">
        <property type="entry name" value="Restrct_endonuc_IV_Mrr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052906">
        <property type="entry name" value="Type_IV_Methyl-Rstrct_Enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30015">
        <property type="entry name" value="MRR RESTRICTION SYSTEM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30015:SF7">
        <property type="entry name" value="TYPE IV METHYL-DIRECTED RESTRICTION ENZYME ECOKMRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04471">
        <property type="entry name" value="Mrr_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Restriction endonuclease type IV Mrr" evidence="1">
        <location>
            <begin position="18"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="715" mass="80119" checksum="709E62A3FF298604" modified="2017-09-27" version="1">MQWTPALIRLASDETLIENVIELLKRMGFREYERVSGKKEWGIDVVAIRDDPIAGTEKVVLALHSKGLASSKDVNVFADLVDKYKADKGIIISPVGFTKDAKVLISREHRGRIVPWDGEKLASLFNNYSLEPPEDLIEALESKADEEKEESSLKEFELDAPLLHEFSPEAVLKRVASAAVSKYPIKSDEVKLDSVSVLLSSAYIFSWSVERDDGTEEKDKAVVFSKERMVLRAIQDKVLSVPITKALLNDGSVIHATEREIDVPISPSEAVFILKETASKELGVPEGRIKIHERKKVYIPKKAELSLRVGENTARAEVDLENDEVRFEISPLPDEYFVERTAAAVEAQTGEAVVDYSLKRAGGKVKVSGKTERFSFELSFNEYTGKLLGMEALMSDEALDELLMSAYPEGQVVNLEKGKKVAVADILIPEGIAVMQVDLTSGKHREARRIPSPETAFGNARKVIEENFPLRNLELRSYRVLEHKYLELNMESSDGKAAVKVDGQTGDILDYVAEVTPERARELASQKYAGFEVTVAESGETEYVLKAENDRHVVTIKVSRDGKLIEEADRVLRREVAEELAERAAKEVDEEAVVKNLALNENWEVEFAGRTKTGKLVLHRATGEVVEKDVRFTEMAIKEAYLAHVKEKHGEENPVVERMTLYEEKGYVHIKVEGKENLYYARIDLKSGKVISEDVAPTKGLTAKLKQFQLENKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9M6</accession>
    <name>A0A218P9M6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tyrosine recombinase XerA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">xerA</name>
        <name evidence="9" type="ORF">A3L08_09260</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 'phage' integrase family. XerA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854734.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9M6"/>
    <dbReference type="GeneID" id="33316458"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09260"/>
    <dbReference type="OrthoDB" id="142231at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009037">
        <property type="term" value="F:tyrosine-based site-specific recombinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00798">
        <property type="entry name" value="INT_XerDC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02055">
        <property type="entry name" value="Recomb_XerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050090">
        <property type="entry name" value="Tyrosine_recombinase_XerCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033686">
        <property type="entry name" value="XerA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040815">
        <property type="entry name" value="recomb_XerA_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30349:SF41">
        <property type="entry name" value="INTEGRASE_RECOMBINASE PROTEIN MJ0367-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30349">
        <property type="entry name" value="PHAGE INTEGRASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02899">
        <property type="entry name" value="Phage_int_SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0229">DNA integration</keyword>
    <keyword evidence="4 5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="8">
        <location>
            <begin position="2"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="7">
        <location>
            <begin position="95"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <feature type="active site" description="O-(3'-phospho-DNA)-tyrosine intermediate" evidence="5">
        <location>
            <position position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="281" mass="32475" checksum="F163E49396F467D1" modified="2017-09-27" version="1">MSEIAEIVEEFGTYLDLEGKSPNTIRMYSYYIRRYLEWGGTLNARSALRFLARLRREGYSNRSLNLVVQALRAYFRFEGADDEAEKLKPPKVPRSLPKALTREEVKRLLSVIPPTKKRDRLIVLLLYGAGLRVSELCNLKRRDIDLERNLIVVRGGKGAKDRVVPIPAFLSDAIRDYLESRDDDSEYLIVEEGRRKKDRLSTKTVWHLLKKYGERAGVEVTPHKLRHSFATHMLENGVDIRAIQELLGHSNLSTTQIYTKVTVEHLRKAQEKARLIEVLMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9N0</accession>
    <name>A0A218P9N0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Probable tRNA sulfurtransferase</fullName>
            <ecNumber evidence="9">2.8.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Sulfur carrier protein ThiS sulfurtransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Thiamine biosynthesis protein ThiI</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">tRNA 4-thiouridine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">thiI</name>
        <name evidence="12" type="ORF">A3L08_09285</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + a uridine in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + ATP + H(+) = [ThiI sulfur-carrier protein]-L-cysteine + a 4-thiouridine in tRNA + 2 oxidized [2Fe-2S]-[ferredoxin] + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:24176"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:13337"/>
            <dbReference type="Rhea" id="RHEA-COMP:13338"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13340"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:136798"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[ThiS sulfur-carrier protein]-C-terminal Gly-Gly-AMP + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + AH2 = [ThiS sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + L-cysteinyl-[cysteine desulfurase] + A + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43340"/>
            <dbReference type="Rhea" id="RHEA-COMP:12157"/>
            <dbReference type="Rhea" id="RHEA-COMP:12158"/>
            <dbReference type="Rhea" id="RHEA-COMP:12910"/>
            <dbReference type="Rhea" id="RHEA-COMP:19908"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:90618"/>
            <dbReference type="ChEBI" id="CHEBI:232372"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ThiI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.4" evidence="9"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854739.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9N0"/>
    <dbReference type="GeneID" id="33316463"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09285"/>
    <dbReference type="OrthoDB" id="372227at2157"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:CCA tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140741">
        <property type="term" value="F:tRNA-uracil-4 sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002937">
        <property type="term" value="P:tRNA 4-thiouridine biosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01712">
        <property type="entry name" value="PPase_ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11716">
        <property type="entry name" value="THUMP_ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1510:FF:000001">
        <property type="entry name" value="Probable tRNA sulfurtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000053">
        <property type="entry name" value="Probable tRNA sulfurtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1510">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2300.10">
        <property type="entry name" value="THUMP superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00021">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020536">
        <property type="entry name" value="ThiI_AANH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054173">
        <property type="entry name" value="ThiI_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049961">
        <property type="entry name" value="ThiI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049962">
        <property type="entry name" value="THUMP_ThiI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003720">
        <property type="entry name" value="tRNA_STrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050102">
        <property type="entry name" value="tRNA_sulfurtransferase_ThiI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006222">
        <property type="entry name" value="PRK08349.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00342">
        <property type="entry name" value="tRNA uracil 4-sulfurtransferase ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43209">
        <property type="entry name" value="TRNA SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43209:SF1">
        <property type="entry name" value="TRNA SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02568">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22025">
        <property type="entry name" value="ThiI_fer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8 9" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 9" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="11">
        <location>
            <begin position="60"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="186"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ07496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="381" mass="43658" checksum="2A7CB4DF4579F8BF" modified="2017-09-27" version="1">MFNVVIVRYGEIGTKSRQTRRWFENLLMNNIREALVSEGIDFKKVEAKHGRVLVKTNRAKEAVEVLTRVFGIVSLSPAMEVDAEMEKINKTALKLFRKKKRELGLEKPRFRVTARRITKEFPLKSPEIQAKVGEYILENEESEVNLHDYDIEVGVELMEGKAYVFVDKVRAWGGLPIGTQGKVVALLSGGIDSPVAAFLMMKRGVEVIPVHIYMGEKTLEKVRKIWNQLKKYHYGGKSELIVVKPKEREKIIQKLKEMKKDKYTCVFCKFMMVRNADKIAKEFGAKGIVMGDSLGQVASQTLENMYIVSQASDLPIYRPLIGMDKEEIVEIAKKIGTFELSTLPEDEIPFIPKHPVIRGSWEEFRKLYKAVFGEEPKKREC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9P8</accession>
    <name>A0A218P9P8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cysteine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_09300</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854742.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9P8"/>
    <dbReference type="GeneID" id="33316466"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09300"/>
    <dbReference type="OrthoDB" id="10138at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="2">
        <location>
            <begin position="3"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="273" mass="30464" checksum="9ABCA24DE7900C0B" modified="2017-09-27" version="1">MSFAKLEFFNPFSRSIKDRTAFNMLLRAMERGDINGTRKLFEASSGNTSISLAALSNVFGIKFRAYLPKPTPKATQVLLRVLGAEVVVTDSETIDQEMVEFVKEEARKEGAANLNQFENDDNFDVHYRVTAREIEEQLQSIGKEPDVLIAGIGTSGHIAGIAKYLKERYDTYVVGVVPARGEKIPGIKRLETGQKWYPLVEIDRVVKVTRREAIEGSIDVARRDGLLIGLSSGAVAKAYERVSEEFGEGTYVLIFPDDGFKYVEVFESYLGMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9S5</accession>
    <name>A0A218P9S5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Metalloprotease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_09545</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854788.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9S5"/>
    <dbReference type="GeneID" id="33316515"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09545"/>
    <dbReference type="OrthoDB" id="10589at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08070">
        <property type="entry name" value="MPN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000085">
        <property type="entry name" value="Mov34/MPN/PAD-1 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028090">
        <property type="entry name" value="JAB_dom_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051929">
        <property type="entry name" value="VirAsm_ModProt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34858">
        <property type="entry name" value="CYSO-CYSTEINE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34858:SF1">
        <property type="entry name" value="CYSO-CYSTEINE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14464">
        <property type="entry name" value="Prok-JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 7" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="6">
        <location>
            <begin position="3"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="132" mass="15035" checksum="3B80F81C7A1E6C74" modified="2017-09-27" version="1">MQLMIRQEALKSIIQVAEKNDLEVCGFLFGRREEETFKVEEVRFITNRLNSPTEFEMDPLEMVEAIDEAEEKGLEVVGIFHSHPKCPPRPSGKDLRGMELWPVAWLIVDGKGNYGAYVLKDGRVEEVSVKAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9S8</accession>
    <name>A0A218P9S8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Large ribosomal subunit protein eL20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpl18a</name>
        <name evidence="3" type="synonym">rpl20e</name>
        <name evidence="3" type="synonym">rplX</name>
        <name evidence="5" type="ORF">A3L08_09490</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Part of the 50S ribosomal subunit. Binds 23S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eL20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854778.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9S8"/>
    <dbReference type="GeneID" id="33316504"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09490"/>
    <dbReference type="OrthoDB" id="191241at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00273">
        <property type="entry name" value="Ribosomal_eL20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028877">
        <property type="entry name" value="Ribosomal_eL20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023573">
        <property type="entry name" value="Ribosomal_eL20_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001981">
        <property type="entry name" value="PRK00773.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01775">
        <property type="entry name" value="Ribosomal_L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160374">
        <property type="entry name" value="RplX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL20" evidence="4">
        <location>
            <begin position="3"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="76" mass="8998" checksum="1627A7CF609F60A4" modified="2017-09-27" version="1">MEVKVFRVKGVFERNGKKERFTREYRGLKKEDVVEILYSEVGSKHRVPRNKIWIEAVEEIKPEEAENPIVRKLSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9X0</accession>
    <name>A0A218P9X0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 14">tRNA (cytidine(56)-2'-O)-methyltransferase</fullName>
            <ecNumber evidence="5 14">2.1.1.206</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 14">tRNA ribose 2'-O-methyltransferase aTrm56</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">A3L08_09705</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 14">Specifically catalyzes the AdoMet-dependent 2'-O-ribose methylation of cytidine at position 56 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>cytidine(56) in tRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(56) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42968"/>
            <dbReference type="Rhea" id="RHEA-COMP:10308"/>
            <dbReference type="Rhea" id="RHEA-COMP:10309"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.206"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4 14">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the aTrm56 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.206" evidence="5 14"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854818.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9X0"/>
    <dbReference type="GeneID" id="33316547"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09705"/>
    <dbReference type="OrthoDB" id="14397at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106059">
        <property type="term" value="F:tRNA (cytidine(56)-2'-O)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002128">
        <property type="term" value="P:tRNA nucleoside ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18083">
        <property type="entry name" value="aTrm56-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00077">
        <property type="entry name" value="tRNA_methyltr_aTrm56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002845">
        <property type="entry name" value="tRNA_mtfrase_aTrm56"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003048">
        <property type="entry name" value="PRK03958.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42197">
        <property type="entry name" value="TRNA (CYTIDINE(56)-2'-O)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42197:SF1">
        <property type="entry name" value="TRNA (CYTIDINE(56)-2'-O)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01994">
        <property type="entry name" value="Trm56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016123">
        <property type="entry name" value="UCP016123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 14 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="9 14 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="11 14" id="KW-0819">tRNA processing</keyword>
    <feature type="binding site" evidence="14">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="112"/>
            <end position="116"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="130"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ASJ07574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="177" mass="19845" checksum="1BAC8C309E54330C" modified="2017-09-27" version="1">MIVILRLGHRPERDKRITTHVALTARAFGADKIIIAAEEDEHVKESVEDVVRRWGGPFGIEFNPSWKKIMREWKDSGGIIAHLTMYGIHIDDALPSIRGELKAGKDLMIVVGAEKVPREVYEMADYNVAVGNQPHSEVAALAVFLDRLLEGEGLRKEFEGAKLKIIPQEKGKKVIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9X3</accession>
    <name>A0A218P9X3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Hydroxide adenosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_09695</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAM hydrolase / SAM-dependent halogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854816.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9X3"/>
    <dbReference type="GeneID" id="33316545"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09695"/>
    <dbReference type="OrthoDB" id="372224at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.90">
        <property type="entry name" value="Bacterial fluorinating enzyme like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10790">
        <property type="entry name" value="S-adenosyl-l-methionine hydroxide adenosyltransferase, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046470">
        <property type="entry name" value="SAM_HAT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046469">
        <property type="entry name" value="SAM_HAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002747">
        <property type="entry name" value="SAM_OH_AdoTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023227">
        <property type="entry name" value="SAM_OH_AdoTrfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023228">
        <property type="entry name" value="SAM_OH_AdoTrfase_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35092">
        <property type="entry name" value="CHLORINASE MJ1651"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35092:SF1">
        <property type="entry name" value="CHLORINASE MJ1651"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20257">
        <property type="entry name" value="SAM_HAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01887">
        <property type="entry name" value="SAM_HAT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006779">
        <property type="entry name" value="UCP006779"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101852">
        <property type="entry name" value="Bacterial fluorinating enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102522">
        <property type="entry name" value="Bacterial fluorinating enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S-adenosyl-l-methionine hydroxide adenosyltransferase N-terminal" evidence="3">
        <location>
            <begin position="2"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosyl-l-methionine hydroxide adenosyltransferase C-terminal" evidence="4">
        <location>
            <begin position="174"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="259" mass="29142" checksum="EC1BA40E6A13A9E7" modified="2017-09-27" version="1">MITLTTDFGLKGPYVGEMKVAMLRVNPNAKLVDVTHAVTRHSIVEGSFVMEQVVKYSPEGTVHVGVIDPGVGTERRAVIVEGEQWLVVPDNGLATLPMKHIKPRRAWEIDLERIRRFTGWKISSTFHGRDVFGPAGALIEKGVSPEEFAEEVPLDSLVRLDLKPRKEKDLWLLKVIYVDDFGNVILNLEDYERLNAVELPDFRLRIPYLDTYGQVKPGELLALPGSHDYLEIAVNQGSASERLGLKVGDEVRVRFVQKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Y9</accession>
    <name>A0A218P9Y9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alanyl-tRNA editing protein AlaX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_01040</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854850.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Y9"/>
    <dbReference type="GeneID" id="33314812"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01040"/>
    <dbReference type="OrthoDB" id="36064at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051335">
        <property type="entry name" value="Alanyl-tRNA_Editing_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43462">
        <property type="entry name" value="ALANYL-TRNA EDITING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43462:SF1">
        <property type="entry name" value="ALANYL-TRNA EDITING PROTEIN AARSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="153" mass="17441" checksum="86B256A76E8623C5" modified="2017-09-27" version="1">MFPIEVRTHTALHVVKGAVVKVLGEGAKWTASTYVKGSRGVLTVKFDRKPTPEEIAEIERLANEKVKEDVPIRVYELPREEAEEHFGEDVYDLFPIPPEVKTLKVVVIEGWNVNACKEEHTRTTGEMGEIKIRKVRFRRSKELLEISFDVVGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA50</accession>
    <name>A0A218PA50_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Endonuclease NucS</fullName>
            <ecNumber evidence="6">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nucS</name>
        <name evidence="9" type="ORF">A3L08_02040</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Cleaves both 3' and 5' ssDNA extremities of branched DNA structures.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NucS endonuclease family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA50"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02040"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22341">
        <property type="entry name" value="NucS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.180.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00722">
        <property type="entry name" value="NucS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002793">
        <property type="entry name" value="Endonuclease_NucS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048301">
        <property type="entry name" value="NucS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048302">
        <property type="entry name" value="NucS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049173">
        <property type="entry name" value="NucS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003270">
        <property type="entry name" value="PRK04247.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38814">
        <property type="entry name" value="ENDONUCLEASE NUCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38814:SF1">
        <property type="entry name" value="ENDONUCLEASE NUCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01939">
        <property type="entry name" value="NucS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21003">
        <property type="entry name" value="NucS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease NucS N-terminal PH-like" evidence="8">
        <location>
            <begin position="2"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease NucS C-terminal" evidence="7">
        <location>
            <begin position="103"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF21003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="223" mass="25335" checksum="521B0C602E361F47" modified="2017-09-27" version="1">MLTIFARCKVHYDGRAKSELGSGDRVIIVKPDGSFLIHQNRKREPVNWQPPGSFVTLEERDGTLVLRSVRRKPKEILEVELDEVYLVSVFRAEDYEELALTGSEAEMAEMIFENPELIEPGFKPLFREKDIGHGIVDILGRDESGNLVVLELKRRKADLHAVSQLKRYVEALKKEHPDVRGILVAPSLTSGAKKLLQKEGLEFKKVEPPKRDRSGRGRQTTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA61</accession>
    <name>A0A218PA61_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Na+/H+ antiporter subunit C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_06565</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA61"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06565"/>
    <dbReference type="OrthoDB" id="18006at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050601">
        <property type="entry name" value="CPA3_antiporter_subunitC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039428">
        <property type="entry name" value="NUOK/Mnh_C1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34583">
        <property type="entry name" value="ANTIPORTER SUBUNIT MNHC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34583:SF2">
        <property type="entry name" value="ANTIPORTER SUBUNIT MNHC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00420">
        <property type="entry name" value="Oxidored_q2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="125" mass="13563" checksum="81E731E9ED91AA37" modified="2017-09-27" version="1">MSFATSLVITAAVATMLIALYGVAVRPNLVKKVLCLSLFSDMVNVLVIFLGYRDVENPLPPVLTEYSSEGVTKLVERSVDPLPQALTITAIVIGLAVTVLMAYGVIHIQRKYGTVDVRKLAGWDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA73</accession>
    <name>A0A218PA73_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_06595</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA73"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06595"/>
    <dbReference type="OrthoDB" id="57329at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08186">
        <property type="entry name" value="Fe-ADH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1970:FF:000003">
        <property type="entry name" value="Alcohol dehydrogenase, iron-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056798">
        <property type="entry name" value="ADH_Fe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045910">
        <property type="entry name" value="AdhA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039697">
        <property type="entry name" value="Alcohol_dehydrogenase_Fe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496:SF102">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25137">
        <property type="entry name" value="ADH_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA" evidence="3">
        <location>
            <begin position="29"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Fe-containing alcohol dehydrogenase-like C-terminal" evidence="4">
        <location>
            <begin position="203"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="405" mass="44897" checksum="57CF577643E8D284" modified="2017-09-27" version="1">MWESHVSINRVFELRCRTTDYFGLCAIHKFNDIVRELKEKGVDKVILVTGRSSYKKCGAWDVVRPALEENGVEYVHYDKVGANPTVDMIDEAAEMGREFGAQAVVGIGGGSPIDSAKSVAILLEYPDKTARDLYEFRFTPTKAKPIIAINTTHGTGTEVDRFAVATIPEKEYKPAIAYDCIYPLYAIDDPALTVKLSREQTLYVTIDALNHITEAATTRLANPYSILLAKEAARLIFDYLPEALKHPDNLQARYALLYASAIAGISFDNGLLHFTHALEHPLSAVKPDLPHGLGLAMLLPAVIRHIYPATAKILAEVYRPLVPEAKGVPGEVELVARRVEEWLFSIGITEKLADVGFTETDVDRLTELAMKTPSLDLLLSMAPVEATRERIAAIYRDSLYPISRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA87</accession>
    <name>A0A218PA87_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Aldehyde ferredoxin oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_07400</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>tungstopterin</name>
            <dbReference type="ChEBI" id="CHEBI:30402"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AOR/FOR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854914.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA87"/>
    <dbReference type="GeneID" id="33316083"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07400"/>
    <dbReference type="OrthoDB" id="30771at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.569.10">
        <property type="entry name" value="Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.599.10">
        <property type="entry name" value="Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.9.10">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013984">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013985">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013983">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036503">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001203">
        <property type="entry name" value="OxRdtase_Ald_Fedxn_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036021">
        <property type="entry name" value="Tungsten_al_ferr_oxy-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051919">
        <property type="entry name" value="W-dependent_AOR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30038">
        <property type="entry name" value="ALDEHYDE FERREDOXIN OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30038:SF5">
        <property type="entry name" value="ALDEHYDE FERREDOXIN OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01314">
        <property type="entry name" value="AFOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02730">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00790">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48310">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, C-terminal domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56228">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0826">Tungsten</keyword>
    <feature type="domain" description="Aldehyde ferredoxin oxidoreductase N-terminal" evidence="10">
        <location>
            <begin position="4"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ07673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="621" mass="68859" checksum="C3AFB7CB354B8129" modified="2017-09-27" version="1">MKGWWGRILRVDLTNNKVWVQEYPEEVAKKFIGGRGLAAWILWNEAKNVDPLGPDNKLVFASGPFNGLPTPSGGKMVVAAKSPLTGGYGDGNLGTMATVHLRKAGYDAIVVEGQAKKPVYLYIEDDNVSILSAEGIWGKGTFETERELKKIHGKNVGVLSIGPAGENLVRYAVVMSQEGRAAGRPGMGAVMGSKKLKAVVIKGTKEIPVADKEKLRELSQEAYNAILNSPGYPFWHRQGTMAAVEWTNENSALPTRNFQDGSFEFARSIDGYTMEGMKVKQRGCPYCNMPCGNVVLDAEGQESELDYENVALLGSNLGIGKLNEVSVLNRIADDMGMDTISLGGAIAFIMEAKEKGLIKDDAAPEFGDFKKAKQLALDIAYRRGELGNLAAEGVMRMAQKLGDDSFAMHVKGLEVSGYNCYIYPAMALAYGTSSIGAHHKEAWVIAWEIGTAPIEGEKAKKAEYKITYDPEKAAKVIELQRLRGGLFEMLTACRLPWVEVGLSLDYYPKLLEAITGVKYTWDDLYNAADRVYALIRAYWVRELGEKWGRHMDYPPKRWFTEGLKSGPYKGQHLDREKYDGLLSEYYRMRGWDERGIPKKETLEKLGLDFVVPELEQVTKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P509</accession>
    <name>A0A218P509_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">KEOPS complex subunit Cgi121</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_00185</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853125.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P509"/>
    <dbReference type="GeneID" id="33314641"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00185"/>
    <dbReference type="OrthoDB" id="85996at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2380.10">
        <property type="entry name" value="CGI121/TPRKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036504">
        <property type="entry name" value="CGI121/TPRKB_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011465">
        <property type="entry name" value="PRK14886.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143870">
        <property type="entry name" value="PF0523-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ05862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="15049" checksum="5FFB4CCB5A106CF7" modified="2017-09-27" version="1">MEEITPNLHIAKVSIKNAETIIPRIKGDFQIVRAECWEEVAFAAILALRSFERGTNHARTLGGELLLRLAGTLQIKDAIAERGIRNGENYLVVFGDRGKAEALLQELGFEELPLTGCERDKLKTFFEKAALVEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P526</accession>
    <name>A0A218P526_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_00355</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853157.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P526"/>
    <dbReference type="GeneID" id="33314675"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00355"/>
    <dbReference type="OrthoDB" id="84835at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055556">
        <property type="entry name" value="DUF7132"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23476">
        <property type="entry name" value="DUF7132"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ05894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="112" mass="13493" checksum="4FFBA7639A5B1337" modified="2017-09-27" version="1">MEVLKEWDVKVKLVKTKRGAILHMIELEPGHFYLEQNPLKDSKYGVAYRKIKENFPEFYMFWEIKNNHYTGKLLAGAFLEKREIDDFITQLAKSEEYKKFENVMENIEELKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P542</accession>
    <name>A0A218P542_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">tRNA methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_00320</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853150.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P542"/>
    <dbReference type="GeneID" id="33314668"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00320"/>
    <dbReference type="OrthoDB" id="30774at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160107">
        <property type="term" value="F:tRNA (adenine(58)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.20:FF:000003">
        <property type="entry name" value="tRNA (Adenine(58)-N(1))-methyltransferase, mitochondrial isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049470">
        <property type="entry name" value="TRM61_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014816">
        <property type="entry name" value="tRNA_MeTrfase_Gcd14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133:SF1">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08704">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14801">
        <property type="entry name" value="TrmI-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017269">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51620">
        <property type="entry name" value="SAM_TRM61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61 C-terminal" evidence="6">
        <location>
            <begin position="74"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="104"/>
            <end position="107"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR017269-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ05887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="253" mass="28659" checksum="DF64E1C1FE0AB02D" modified="2017-09-27" version="1">MIREGDKVLLIDRRGKRYLVTVSDREFHTDLGILKLGELIGKPYGSSITSHKGEEFRVIKPDINDIIAKMRRGPQIIHPKDAGIIIAYAGISPGDTVIEAGVGSGALTIFLANIVGPSGRVISYEIREDHAEIARKNVELAGFSDRVTIKLKDIYEGIDEERADHIVLDLPQPENVLPHAVEVLRPGGYFVAYTPCANQVHRFFQAFQEYREYFYRPRVVEVLVREQEIKKECMRPKTTMLAHTGYITFIRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P552</accession>
    <name>A0A218P552_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TrmB family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853154.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P552"/>
    <dbReference type="GeneID" id="33314672"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00340"/>
    <dbReference type="OrthoDB" id="94698at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="1">
        <location>
            <begin position="12"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="109" mass="12571" checksum="2A1731A4BC062B5D" modified="2017-09-27" version="1">MEDVLRTLARSLKFFELGETEIRIYSLLQRESLTPRQIAKALGLSERIVREKLKHLLELGLIERTLVNRGWLGYVYHAKAPQKALNELLSRIESTLKALENEVQTNLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P553</accession>
    <name>A0A218P553_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_00520</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853187.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P553"/>
    <dbReference type="GeneID" id="33314708"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00520"/>
    <dbReference type="OrthoDB" id="9573at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.30.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002878">
        <property type="entry name" value="ChsH2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022002">
        <property type="entry name" value="ChsH2_Znr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052513">
        <property type="entry name" value="Thioester_dehydratase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075:SF5">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01796">
        <property type="entry name" value="OB_ChsH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12172">
        <property type="entry name" value="zf-ChsH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ChsH2 rubredoxin-like zinc ribbon" evidence="2">
        <location>
            <begin position="18"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="ChsH2 C-terminal OB-fold" evidence="1">
        <location>
            <begin position="51"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ05924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="138" mass="16034" checksum="C311409E64F97746" modified="2017-09-27" version="1">MGKPMQVARHWRHFEEKYALIGGKCENGHLMFPYREICPVCGSRNVEKHKFSGKGKVVSWTIVRNPPSGFEYYKPYPLALVQLEEGPVVLAQLTDVEPEEIDFGMEVEMVTRKVREFDEDGIILYAYKFRPVLKPQKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P554</accession>
    <name>A0A218P554_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF2357 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853179.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P554"/>
    <dbReference type="GeneID" id="33314699"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00475"/>
    <dbReference type="OrthoDB" id="67718at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018633">
        <property type="entry name" value="DUF2357"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007505">
        <property type="entry name" value="PDDEXK_7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09823">
        <property type="entry name" value="DUF2357"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04411">
        <property type="entry name" value="PDDEXK_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2357" evidence="1">
        <location>
            <begin position="68"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="544" mass="62278" checksum="A28804125C85D6D2" modified="2017-09-27" version="1">MECIDGTPLDGWRIFSAKAGCRAFKGKDKLYLIEWTDYYVEGTNNSLKTVLINGKPARRLKNGLFVVSFGNFVGLSELVLLFESGKTTKVPVEVLSLKVSRMYPELFSSFSGHWIERLSRLQNAFVETLTGEILQYSSAIPFHLESPTGFSTLESDEPINELFAYRFLRSNGERIIEAFETVLRRMKRKLVVEEELLRPDEVDEITPEALLSIVQHPEYLAPAGEGVLIADYLNGYAPTKVLGYMKYESFDTLENRFAKYFLNLLIEWSERVIETLGEKKANLGPIKELRGELEFIGSDGVWDDVGGMTLFHYTSQTLLKGDGYRDLLELYREFTARVPFFEELQRATDNKDIAKLYEYWAFFGLVEELGEILGKKELKVRILPSGELSERGDVYAEFDNGWRLYYNKPLSPTRWSYSVSLRPDFSLFNGNPSKKSTELIGVFDAKFKLDVVDKPKKIEEFDEETETAEKTGNYETWAKLEDVYKMHTYRDALGCRFAVVVYPGERSVFFDMNRGKVGGFALEEVLTGELNGVGYLRLVPEVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P561</accession>
    <name>A0A218P561_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cation:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_00390</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853163.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P561"/>
    <dbReference type="GeneID" id="33314682"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00390"/>
    <dbReference type="OrthoDB" id="19265at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050622">
        <property type="entry name" value="CPA3_antiporter_subunitB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007182">
        <property type="entry name" value="MnhB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006249">
        <property type="entry name" value="PRK08387.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33932">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33932:SF4">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04039">
        <property type="entry name" value="MnhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Na+/H+ antiporter MnhB subunit-related protein" evidence="7">
        <location>
            <begin position="6"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ05900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="133" mass="14414" checksum="0D85CA40DFDD9577" modified="2017-09-27" version="1">MKMSTVVRTTTKMVSPFLVTYAAYLMLYGHMSPGGGFQGGVILAVAVILLITSHGYAKVRRKFHFNWASLIESSAGVLLVLLGLTGVAFGAFYANFLPTEGGIILPFNVIVGLEVGAAFTFVFYILLRWVESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P592</accession>
    <name>A0A218P592_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0282 protein A3L08_00745</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_00745</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0282 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853225.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P592"/>
    <dbReference type="GeneID" id="33314753"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00745"/>
    <dbReference type="OrthoDB" id="21331at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01406">
        <property type="entry name" value="UPF0282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050114">
        <property type="entry name" value="UPF0173_UPF0282_UlaG_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014426">
        <property type="entry name" value="UPF0282_hydrls"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003290">
        <property type="entry name" value="PRK04286.1-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43546">
        <property type="entry name" value="UPF0173 METAL-DEPENDENT HYDROLASE MJ1163-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43546:SF4">
        <property type="entry name" value="UPF0282 PROTEIN MJ1629"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004944">
        <property type="entry name" value="UCP004944_hydrls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="2">
        <location>
            <begin position="14"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ05963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="299" mass="34097" checksum="A2D96FC3FDB30040" modified="2017-09-27" version="1">MRLIPLAAESLGVRSLATFVEASGLKILIDPGVALGPKRYGLPPTKIELETLQQMRRKLQGYARRADVVTISHYHYDHHTPFFEGLYESSSEEYAREIYAGKLLFIKHPRENINFSQRKRAWAFLKNAEPIAKKIEFSDGRTFDLGGVRLEFSPAVPHGSEGSKLGFVVMVLIDDGSKSVIHASDIQLLNREAVEWIVEKNPDLLITGGPPTYLGKRAEGSWETGIKNLNEIIRETDAEIILDHHIVRDKRYPEFFDGLEKKPKTFAGYLKVEDRPLEAYRRELHKRERGEETELPFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P593</accession>
    <name>A0A218P593_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_00645</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853210.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P593"/>
    <dbReference type="GeneID" id="33314733"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00645"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17325">
        <property type="entry name" value="MFS_MdtG_SLC18_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23518">
        <property type="entry name" value="C-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23518:SF2">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="304"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="328"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="363"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="391"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="28"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ05948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="412" mass="44915" checksum="F85EE7F274DA7A80" modified="2017-09-27" version="1">MPKRERVVIQGKRERTLKLKKLRVKRRNILILAIAMFIANVAFGMAFPYLSVYMSLLGASMFMVGLLSVAFNLTSTVFQYPFGWLSDSTGNRKAFIAFGAASIGVFYTAIAFVNSATGVLILRTLQGVFGSAMTPAHSALISELSTRAGSIFGLFNSIENAGYMVGNFLGSFVVEYLGIKSVFVIAGFLLFLSAGLVLLIRERPSGKRSILGMILVQEGRESWRATVKGSAFKELMRGHLGLFYVTVFLVMIASGQFYSVSSVYFKETFGEWSVGVIFGVESLAAALTGYFLGRLIDRYGAKRFYLLAIAGYGLAFLLYAFVKNVWLVFGVAFLSGVKWILTINSTSAYVAQNVKVSERAQGMGLLNAMMSLGWVVGPLIGGYLAGISFTLNFLSTLVPLGLAFLLALKLQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P597</accession>
    <name>A0A218P597_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribose 1,5-bisphosphate isomerase</fullName>
            <shortName evidence="4">R15P isomerase</shortName>
            <shortName evidence="4">R15Pi</shortName>
            <ecNumber evidence="4">5.3.1.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Ribulose 1,5-bisphosphate synthase</fullName>
            <shortName evidence="4">RuBP synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_00550</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the isomerization of ribose 1,5-bisphosphate (R15P) to ribulose 1,5-bisphosphate (RuBP), the CO(2) acceptor and substrate for RubisCO. Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and RubisCO.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>alpha-D-ribose 1,5-bisphosphate = D-ribulose 1,5-bisphosphate</text>
            <dbReference type="Rhea" id="RHEA:32243"/>
            <dbReference type="ChEBI" id="CHEBI:57870"/>
            <dbReference type="ChEBI" id="CHEBI:68688"/>
            <dbReference type="EC" id="5.3.1.29"/>
        </reaction>
    </comment>
    <comment type="miscellaneous">
        <text evidence="4">Reaction proceeds via a cis-phosphoenolate intermediate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eIF-2B alpha/beta/delta subunits family. R15P isomerase subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.29" evidence="4"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853193.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P597"/>
    <dbReference type="GeneID" id="33314714"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00550"/>
    <dbReference type="OrthoDB" id="27639at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043917">
        <property type="term" value="F:ribose 1,5-bisphosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046523">
        <property type="term" value="F:S-methyl-5-thioribose-1-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019323">
        <property type="term" value="P:pentose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.420:FF:000011">
        <property type="entry name" value="Ribose 1,5-bisphosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10470:FF:000019">
        <property type="entry name" value="Ribose 1,5-bisphosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.420">
        <property type="entry name" value="translation initiation factor eif-2b, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02230">
        <property type="entry name" value="R15P_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011559">
        <property type="entry name" value="Initiation_fac_2B_a/b/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027363">
        <property type="entry name" value="M1Pi_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005250">
        <property type="entry name" value="R15Pi"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00524">
        <property type="entry name" value="eIF-2B_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00511">
        <property type="entry name" value="ribulose_e2b2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475:SF2">
        <property type="entry name" value="RIBOSE 1,5-BISPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="20"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="135"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="212"/>
            <end position="213"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ05930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="322" mass="36198" checksum="343759CB14D7E42B" modified="2017-09-27" version="1">MAVVKEVLEIAQEIKDMRIRGAGKIARSAAYALQLQAEKSTAKDVDEFWNEMKQAAKILYETRPTAVSLPNALRYVMHRGKVAYSSGADLEELRYVIITAAKEFIHNSEKAVERIGEIGAKRIEDGDVIMTHCHSKAALSVMKKAWDQGKDIKVIVTETRPKWQGKITAKELADYGIPVIYVVDSAARHYMKMTDKVVMGADSITVNGAVINKIGTALIALTAKEHRVWTMIAAETYKFHPDTMLGQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYVDVIITERGIIPPSAAIDILKEEFGWALKYSEPWED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5D3</accession>
    <name>A0A218P5D3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Diphthine synthase</fullName>
            <ecNumber evidence="6">2.1.1.98</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Diphthamide biosynthesis methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">dphB</name>
        <name evidence="9" type="ORF">A3L08_00780</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 3 S-adenosyl-L-methionine = diphthine-[translation elongation factor 2] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:36415"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10172"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="ChEBI" id="CHEBI:82696"/>
            <dbReference type="EC" id="2.1.1.98"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the diphthine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.98" evidence="6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853232.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5D3"/>
    <dbReference type="GeneID" id="33314760"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00780"/>
    <dbReference type="OrthoDB" id="39139at2157"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004164">
        <property type="term" value="F:diphthine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11647">
        <property type="entry name" value="DHP5_DphB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.950.10:FF:000004">
        <property type="entry name" value="Diphthine synthase putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1010.10:FF:000004">
        <property type="entry name" value="Putative diphthine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01084">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004551">
        <property type="entry name" value="Dphthn_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00522">
        <property type="entry name" value="dph5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882:SF0">
        <property type="entry name" value="DIPHTHINE METHYL ESTER SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882">
        <property type="entry name" value="DIPHTHINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036432">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="8">
        <location>
            <begin position="3"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <begin position="115"/>
            <end position="116"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR036432-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ05970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="264" mass="29735" checksum="C9571B8CABDA363C" modified="2017-09-27" version="1">MAIYFIGLGLYDERDITLKGLEIARKCDTVFAEFYTSLLAGTTMDKIEELIGKPIRRLSREDVELNFEKIVLSEAKDKDVAFLTAGDPMVATTHSDLRIRAKQNGIESYVIHAPSIYSAIAITGLHIYKFGKSATVAYPEKNWFPTSHYDVIRENMERGLHTMLFLDIKADQNRYMTANEAMEILLQVEEMKGQGVFTPDTLVVVLARAGSLNPTLRAGYVKDMINEDFGKQPHVMVVPGRLHIVEAEYLVEFAGAPREILEET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5D6</accession>
    <name>A0A218P5D6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF366 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_00905</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853254.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5D6"/>
    <dbReference type="GeneID" id="33314785"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00905"/>
    <dbReference type="OrthoDB" id="70011at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007162">
        <property type="entry name" value="DUF366"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04017">
        <property type="entry name" value="DUF366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006503">
        <property type="entry name" value="UCP006503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ05992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="21167" checksum="14F7E2FCD4AF2992" modified="2017-09-27" version="1">MELLVVKDKRIDYDGSAIQSHWVYRNFGILGNSLVVFRGKCDVKVEEMIDIEDLRQSKEIKSDDMVHYIIEVFDLVNTLFASTLQKLFIAKLCEVLEEYGVKTSRKGDDIYVNGKKLSISIATVSPVSVKIHIGINVEAKGIPEGVDAIGLKELGITDVEDFMERTGKALVAEFEKVKKDSLKVRWAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5G7</accession>
    <name>A0A218P5G7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF835 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_00965</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5G7"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00965"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008553">
        <property type="entry name" value="DUF835"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05763">
        <property type="entry name" value="DUF835"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF835" evidence="2">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="176" mass="19435" checksum="D8644C3785D46BE0" modified="2017-09-27" version="1">MVSRNTYDQWVKKSGVEPDKFIWLSRAELEGAVDPGKLHVLQREILAFLETQSPASVYFEGVEYLVLYNDFPGVAKFLFSVKDAVLINNSLMLLFLPKGVLDSKQESVLAREFEPIDEKELTRRILNALPEKERAEITLFGVLPPAGGRESEGSKGAGAESSEEGSGAGEEKTEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5J0</accession>
    <name>A0A218P5J0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable phosphoglucosamine mutase</fullName>
            <ecNumber evidence="6">5.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 12" type="primary">glmM</name>
        <name evidence="12" type="ORF">A3L08_01120</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>alpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23424"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="5.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="6">Activated by phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.10" evidence="6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853292.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5J0"/>
    <dbReference type="GeneID" id="33314828"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01120"/>
    <dbReference type="OrthoDB" id="10363at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008966">
        <property type="term" value="F:phosphoglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03087">
        <property type="entry name" value="PGM_like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000001">
        <property type="entry name" value="Phosphoglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.50:FF:000009">
        <property type="entry name" value="Probable phosphoglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01554_A">
        <property type="entry name" value="GlmM_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023666">
        <property type="entry name" value="GlmM_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024086">
        <property type="entry name" value="GlmM_arc-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03990">
        <property type="entry name" value="Arch_GlmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43771">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43771:SF1">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 6 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="9">
        <location>
            <begin position="3"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="10">
        <location>
            <begin position="151"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="11">
        <location>
            <begin position="254"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase C-terminal" evidence="8">
        <location>
            <begin position="365"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="6">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="binding site" description="via phosphate group" evidence="6">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="6">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="451" mass="49188" checksum="D6AF1D32403D5E60" modified="2017-09-27" version="1">MGKYFGTSGIREVVNEKLTPELALRVGKALGTYLGGGTVVVGKDTRTSGEMLKNALISGLLSVGVEVIDIGLAPTPLTGFAIRLYGADAGVTITASHNPPEYNGIKVWQPNGMAYTPEMEAQLEAIIDSGNFKKAPWNEIGRVRKADPRKEYIKRALEMVHLDGSYTVVLDAGNGAGSIISPYLQRELGNKVISLNSHPSGFFVRELEPNAKSLSALAKTVKAMNADVGIAHDGDADRIGVVDDRGNFVEYEVMLSLIAGYMLRKFGKGKVVTTVDAGFALDDYVRPLGGEVIRTRVGDVAVADELARHGGVFGGEPSGTWIIPQWNLTPDGIFAGALVMEMVDKLGPLSELAREVPRYVTLRAKIPCPNEKKAKAMEIIAREALKTFDYKRLIDIDGVRIENDEWWILFRPSGTEPIMRITLEAHTEEKARELMERAKRLVKKAIGDIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5J4</accession>
    <name>A0A218P5J4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pyruvate ferredoxin oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">porA</name>
        <name evidence="5" type="ORF">A3L08_01315</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of one alpha, one beta, one delta and one gamma chain.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853327.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5J4"/>
    <dbReference type="GeneID" id="33314868"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01315"/>
    <dbReference type="OrthoDB" id="372068at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016903">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000010">
        <property type="entry name" value="Pyruvate ferredoxin oxidoreductase, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000012">
        <property type="entry name" value="Pyruvate:ferredoxin (Flavodoxin) oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050722">
        <property type="entry name" value="Pyruvate:ferred/Flavod_OxRd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006232">
        <property type="entry name" value="PRK08366.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154">
        <property type="entry name" value="PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154:SF0">
        <property type="entry name" value="PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate flavodoxin/ferredoxin oxidoreductase pyrimidine binding" evidence="3">
        <location>
            <begin position="19"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate:ferredoxin oxidoreductase core" evidence="4">
        <location>
            <begin position="264"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="394" mass="44050" checksum="1D437BF9C0F19971" modified="2017-09-27" version="1">MEYKPIRKVVSGNYAAAYAAKHARVQVVAAYPITPQTSIIEKIAEFIANGEADIQYVPVESEHSAMAACIGASAAGARAFTATSAQGLALMHEMLHWASGARLPVVMVDVNRAMAPPWSVWDDQTDSLAQRDTGWLQFYAENNQEVYDGVLMAFKVAETVNVPAMVIESAFILSHTYDVVEMIPQELVDEFLPPREPLYDLANFEKPFSVGALGTPADYYEFRYKLAKAHEEAKKVIKEVGKEFGERFGRDYSQMIETYRTDDADFVFMGMGSLMGTVKQAVDVLREEGYKVGAAKVRWFRPFPKEELYELAKNVDGIAVLDRNFSFGQEGILFNEAKGALYNTGAKPLMKNYIVGLGGRDFTVNDVRKIAENMKAIIEKGELDVEVDWYHLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5K2</accession>
    <name>A0A218P5K2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Multidrug ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_01375</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853337.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5K2"/>
    <dbReference type="GeneID" id="33314880"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01375"/>
    <dbReference type="OrthoDB" id="85744at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047817">
        <property type="entry name" value="ABC2_TM_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051784">
        <property type="entry name" value="Nod_factor_ABC_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43229:SF6">
        <property type="entry name" value="ABC-TYPE MULTIDRUG TRANSPORT SYSTEM, PERMEASE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43229">
        <property type="entry name" value="NODULATION PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00164">
        <property type="entry name" value="ABC2TRNSPORT"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="66"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="113"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-2" evidence="6">
        <location>
            <begin position="33"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="272" mass="30013" checksum="6C4321E74E9AE5E1" modified="2017-09-27" version="1">MVATELRALWGVAVKSWRIFLSYRVWFISDIAMGFFFVGQALLIGIGLTGERNSEALQELTGYSDYVTFAVLGFMVLGFGLTFLSGFVWSVVDELYAGTLEYSFAAPMRRLTFFLGNVLTRILLSFVYMAFYVPAFVLLFGLSFDPVAFSRAIPVLLLGTVGMIGMGMAAAGIVLYLRDPGPFISILEMLVFALSGAMYPVSILPKGLQYLAKALPYSPTTEAVRKVVAYGYGASISEITYLLLVSLTYGLLGYLTYRWSEKQARVVGLKTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5T4</accession>
    <name>A0A218P5T4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_01820</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853416.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5T4"/>
    <dbReference type="GeneID" id="33314969"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01820"/>
    <dbReference type="OrthoDB" id="26307at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11717">
        <property type="entry name" value="THUMP_THUMPD1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2130.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040183">
        <property type="entry name" value="THUMPD1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="2">
        <location>
            <begin position="49"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="161" mass="17684" checksum="B0499A0CB235C4AA" modified="2017-09-27" version="1">MTVLIATVPQGREGDGILELEWALGEVRVKGTDWGGVLLAETPLSKEGALRRLGEFETQAIQRVVPLDEFVPAKWEEIEEAVLRLAGEIQGTFAVRAKVRGNKRISARALEVKLGSLIVERFGLPVNLTDPDFTVVIEVLGKRAGVGVLKRGELLRFEVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5T7</accession>
    <name>A0A218P5T7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cation:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_01765</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853405.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5T7"/>
    <dbReference type="GeneID" id="33314958"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01765"/>
    <dbReference type="OrthoDB" id="18006at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050601">
        <property type="entry name" value="CPA3_antiporter_subunitC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039428">
        <property type="entry name" value="NUOK/Mnh_C1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006250">
        <property type="entry name" value="PRK08388.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34583">
        <property type="entry name" value="ANTIPORTER SUBUNIT MNHC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34583:SF2">
        <property type="entry name" value="ANTIPORTER SUBUNIT MNHC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00420">
        <property type="entry name" value="Oxidored_q2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="119" mass="13031" checksum="5BCAFF4E85076590" modified="2017-09-27" version="1">MIQFQFITAFLLIALGIYALLAKRNLLKLVLALDIIDSGIHLLLVSLGYRIELNEVPTAPIYTGYETLKSPMVGPLPQALVLTSIVIGVCVLALAVALTVNAYRHYGTLDVRALRRLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5V1</accession>
    <name>A0A218P5V1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853418.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5V1"/>
    <dbReference type="GeneID" id="33314971"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01830"/>
    <dbReference type="OrthoDB" id="350145at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007404">
        <property type="entry name" value="YdjM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04307">
        <property type="entry name" value="YdjM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="176" mass="18934" checksum="F82DFE728BCC928E" modified="2017-09-27" version="1">MPNYDAHVLSGIVSYPIAVAVAGYLSTIGAPFKLTTIALVIGYALYVLGADLPDMDHPDALIHRGTKPIVSVLTGGAAYINVIDRIHLNPEWLTITAAWGIAIAAALVAWYAFTWLMPKHRGIVHSLLFAFIYGALAFLLVDYGLGMSTGEGLYVGFAAFSGYTLHLLLDREISLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5V6</accession>
    <name>A0A218P5V6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ECF transporter S component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5V6"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01850"/>
    <dbReference type="OrthoDB" id="31497at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1760.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009825">
        <property type="entry name" value="ECF_substrate-spec-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37815">
        <property type="entry name" value="UPF0397 PROTEIN BC_2624-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37815:SF3">
        <property type="entry name" value="UPF0397 PROTEIN SPR0429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07155">
        <property type="entry name" value="ECF-ribofla_trS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="20737" checksum="EDA8334EE139C131" modified="2017-09-27" version="1">MSELTEILKPYGPYVLAGVTLLYVLYLYLNRKKFKSASVLAISAVMAAVVGVTTAFITISTPATGGYLNFGDTMVMFSAMVFGPVVGVFAGGVGSALGDIIAGYPGWAPITLVVKGLEGLVIGYLARKSDNVGNLIVAGVVGGMIMVLGYFSFEAYMYGVPAAATEIPVNTLQAVTGVIVGTGLAKAIKKRYPEVEDFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5W0</accession>
    <name>A0A218P5W0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TIGR00153 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_01705</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0111 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853395.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5W0"/>
    <dbReference type="GeneID" id="33314946"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01705"/>
    <dbReference type="OrthoDB" id="123511at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="entry name" value="Phosphate transport system protein phou homolog 2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002727">
        <property type="entry name" value="DUF47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018445">
        <property type="entry name" value="Put_Phosphate_transp_reg"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00153">
        <property type="entry name" value="TIGR00153 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36536">
        <property type="entry name" value="UPF0111 PROTEIN HI_1603"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36536:SF3">
        <property type="entry name" value="UPF0111 PROTEIN HI_1603"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01865">
        <property type="entry name" value="PhoU_div"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109755">
        <property type="entry name" value="PhoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="224" mass="25516" checksum="09674D4D20C5061F" modified="2017-09-27" version="1">MQVWTKLFAKSPFKPLIKHSEVVLNTVETLERALQAWERGDYEEMKRLAVEVDRLEDVADRIKEEIRDSLSSKLMMAVAREDVLIYLHMQDKVADAAEDTAKWLLVKKPGTIPAEIKEIIIQMGAESIKAAKLVHKAIVQMDRVIESGFSENEIKAEYEIIHEIEGVESKIDGLDTKLMQKVFEMADEMSWGDGICILNVARTLSNISDKAKDAAERIRLMMNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5X6</accession>
    <name>A0A218P5X6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tRNA (cytosine(72)-C(5))-methyltransferase</fullName>
            <shortName evidence="5">tRNA:m(5)C72 MTase</shortName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_01955</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in several tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>cytidine(72) in tRNA + S-adenosyl-L-methionine = 5-methylcytidine(72) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61988"/>
            <dbReference type="Rhea" id="RHEA-COMP:15996"/>
            <dbReference type="Rhea" id="RHEA-COMP:15997"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74483"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853442.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5X6"/>
    <dbReference type="GeneID" id="33314996"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01955"/>
    <dbReference type="OrthoDB" id="14725at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016428">
        <property type="term" value="F:tRNA (cytidine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07953">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02237">
        <property type="entry name" value="NSUN6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011023">
        <property type="entry name" value="Nop2p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043699">
        <property type="entry name" value="NSUN6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054728">
        <property type="entry name" value="RsmB-like_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00446">
        <property type="entry name" value="nop2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF34">
        <property type="entry name" value="TRNA (CYTOSINE(72)-C(5))-METHYLTRANSFERASE NSUN6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22458">
        <property type="entry name" value="RsmF-B_ferredox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="7">
        <location>
            <begin position="114"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5 6">
        <location>
            <position position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <begin position="206"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="385" mass="43618" checksum="02A37741570C8BB1" modified="2017-09-27" version="1">MSYEDAFPPELREYYRRLFGSEAEEIMASLRTPVEKYYIRVNTLKTSRSKLMSILKKEGLKPKRSPYLKEGIYFEREGPNFPDDYEPGLPVVRANKFASESVYQGAMLYAPGVLQADKKIKPGDEVEIRDPRGLLVGVGIARMSAKEMILSTRGLAVEVTLPKFRLPSLSELESFREGLFYAQSLPSMVVAHVLEPSEEELIIDMAAAPGGKTSHISQLMQNRGEIIAIDKSRNRLKKTEEELNRLGVKNVKLLHMDSRKLPELGIEADKILLDAPCTALGIRPKLWESRTPKDIEATARYQRHFINAAIKSLRKDGVLVYSTCTLSLEENEANVRYMLEKGLKLEEQSVFIGSHGIDLDGVQRFYPNRHLTQGFFIARLRKVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Z5</accession>
    <name>A0A218P5Z5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_02070</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5Z5"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02070"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="263" mass="29990" checksum="73A7B6F8E920DB9E" modified="2017-09-27" version="1">MKRAGLLAGVLVLIFVFSGFVGARPQADVDSWTAVDLFVREQIDMACAKGTINDPHFVEGLTDLVLKRFDRQLDAEIAEHAKNLTLEQRETLKRLLVSDYVSYLYQKSFYPKNAKVLVVKPVKVASVKANGKVYDYYYAPMSSSILYPIHWWVQVKDDLEGGHGKDGHGNYYWIDGNNNISYINVIYASSNVVYEIHFYGEDDPDPVMDEYYDWQRCRNYPGRDDTYGCEDIEAIEVSSGDIIFHSDWSHVCDDGMCGSDICR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P601</accession>
    <name>A0A218P601_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tryptophan--tRNA ligase</fullName>
            <ecNumber evidence="9">6.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Tryptophanyl-tRNA synthetase</fullName>
            <shortName evidence="9">TrpRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">trpS</name>
        <name evidence="12" type="ORF">A3L08_02120</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the attachment of tryptophan to tRNA(Trp).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>tRNA(Trp) + L-tryptophan + ATP = L-tryptophyl-tRNA(Trp) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24080"/>
            <dbReference type="Rhea" id="RHEA-COMP:9671"/>
            <dbReference type="Rhea" id="RHEA-COMP:9705"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78535"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="9"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853471.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P601"/>
    <dbReference type="GeneID" id="33315029"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02120"/>
    <dbReference type="OrthoDB" id="371821at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006436">
        <property type="term" value="P:tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00806">
        <property type="entry name" value="TrpRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.240.10:FF:000007">
        <property type="entry name" value="Tryptophan--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000138">
        <property type="entry name" value="Tryptophan--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00140_A">
        <property type="entry name" value="Trp_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020653">
        <property type="entry name" value="Tryptophan-tRNA-ligase_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008924">
        <property type="entry name" value="PRK12285.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008927">
        <property type="entry name" value="PRK12285.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00233">
        <property type="entry name" value="trpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10055:SF1">
        <property type="entry name" value="TRYPTOPHAN--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10055">
        <property type="entry name" value="TRYPTOPHANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 9 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="339"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="9">
        <location>
            <begin position="81"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="9">
        <location>
            <begin position="252"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="384" mass="44689" checksum="93A81911E422EFF2" modified="2017-09-27" version="1">MDDFKVTPWDVEGVVDYAKLIVEFGTSPLTDELIQKTAELTKSELPIFFRRRFFFSHRDYDKVLADYESGKGFFLYTGRGPSGPMHIGHIIPFFATKWLQEKFDVNLYIQITDDEKFLFKEKLTFDETKRWAYENILDIIAVGFDPDRTFIFQNSEFTKIYEMAIPIAKKINFSMAKAVFGFNEQSKIGMIFYPAIQAAPTFFEKKRCLIPAAIDQDPYWRLQRDFAESLGYYKTAAIHSKFVPGLTSLGGKMSASKPETAVYLTDDPEEAGKKIWKFALTGGRATAKEQRENGGEPEKCVVFKWFEIFFEPDDEKLMERYRACKSGELLCGQCKRELIERVQKFLKEHQERRKKAEKQVEKFKYTGELAREQWDKAVPEALKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6B5</accession>
    <name>A0A218P6B5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0216 protein A3L08_02700</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0216 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853579.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6B5"/>
    <dbReference type="GeneID" id="33315146"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02700"/>
    <dbReference type="OrthoDB" id="18795at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00585">
        <property type="entry name" value="UPF0216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002746">
        <property type="entry name" value="UPF0216"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003153">
        <property type="entry name" value="PRK04115.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01886">
        <property type="entry name" value="DUF61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005264">
        <property type="entry name" value="UCP005264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="135" mass="15830" checksum="D1DCF2A50AAF6D58" modified="2017-09-27" version="1">MPTAEDILNREVARVNLHLPAFRPTLSRLLAEEEPRVKLRDGSYHYFKRSELEYLASLLEEEETERLKVPIVLEISTVYRGYFRVRGAVEVKVIDKILGTYDILEEKSEELYPRYLLPKIRRALPTTTTYAFIAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6C8</accession>
    <name>A0A218P6C8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Fe-S protein, radical SAM family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_02845</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853607.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6C8"/>
    <dbReference type="GeneID" id="33315175"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02845"/>
    <dbReference type="OrthoDB" id="372128at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058374">
        <property type="entry name" value="DUF8061"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040087">
        <property type="entry name" value="MJ0021-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43288">
        <property type="entry name" value="BIOTIN SYNTHASE-RELATED PROTEIN, RADICAL SAM SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43288:SF1">
        <property type="entry name" value="GLYCYL-RADICAL ENZYME ACTIVATING ENZYME MJ0021-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26257">
        <property type="entry name" value="DUF8061"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01108">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="21"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="360" mass="41575" checksum="D71E8CF1C17EEA40" modified="2017-09-27" version="1">MVRETPYFSYAVRELPKGCQLCVRGEKLVLFTTGVCPRDCFYCPLSPWRREDVVYANERPGKRVDDVIEEALLQEAKGAGVTGGDPFARLDRTVEYIKLLKENFGEEFHIHLYTTGALATKRNLEKLYDAGLDEIRFHPDLFNPNSKLFKVEIENIKNAFDFDWDVGGEIPSIPGQFERMKWYAEFLDKLGAKFLNVNELEYSEMTLKTLLDLGYQPVSDESASIKGSLETGLKLLEWGEENTSLSYHLCTARLKDAVQLRNRLKRMARKVAKPYMEITEEGTLRFGIAEYDDLDELYSFLVKEAEVPPEWLHINREKGRIEMPEEVAVELAEAIEGDVKFFIVEEYPTWDRLEVERTPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6D8</accession>
    <name>A0A218P6D8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_02720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853582.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6D8"/>
    <dbReference type="GeneID" id="33315150"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02720"/>
    <dbReference type="OrthoDB" id="50325at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF102">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="7"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="151"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="282" mass="30747" checksum="7C99DF9796E09335" modified="2017-09-27" version="1">MNGRVKIATAMLIWGSVGIFARFSGLSGLGVAFARVSLGSILLLTIMGLLEKEKLAELPGLLRQQWKPFIGLGIALALNWAFLFTAFNYTTIANAVLVYYTSPILAMLISWRFLNERIAGKRWALIGMAFLGLALIISGQRLSLNDRDFVGILLAFVAAFFYALIPNFGRFLRDVDGKVLTFLQLAIASFVLAPFVAVQDTGTPVWWAVLVLVVVHTVLALFLYMDGLKTVEVNEAALLSYLDPMSAVVYAFLVFGEVPGFTTAMGGALILLASALDILSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6F0</accession>
    <name>A0A218P6F0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Vitamin B12-dependent ribonucleotide reductase</fullName>
            <ecNumber evidence="12">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">A3L08_02945</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + [thioredoxin]-disulfide + H2O = a ribonucleoside 5'-diphosphate + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ribonucleoside diphosphate reductase class-2 family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="12"/>
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        <property type="protein sequence ID" value="ASJ06362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A218P6F0"/>
    <dbReference type="GeneID" id="33315195"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02945"/>
    <dbReference type="OrthoDB" id="6188at2157"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:cobalamin binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="RNR_II_dimer"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Vitamin B12-dependent ribonucleotide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050862">
        <property type="entry name" value="RdRp_reductase_class-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
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        <property type="entry name" value="RNR_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013344">
        <property type="entry name" value="RNR_NrdJ/NrdZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008926">
        <property type="entry name" value="RNR_R1-su_N"/>
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    <dbReference type="NCBIfam" id="TIGR02504">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE"/>
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    </dbReference>
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        <property type="entry name" value="VITAMIN B12-DEPENDENT RIBONUCLEOTIDE REDUCTASE"/>
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    </dbReference>
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        <property type="entry name" value="ATP-cone"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00317">
        <property type="entry name" value="Ribonuc_red_lgN"/>
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    </dbReference>
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        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
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    </dbReference>
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        <property type="entry name" value="R1 subunit of ribonucleotide reductase, N-terminal domain"/>
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    </dbReference>
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    <keyword evidence="3 12" id="KW-0846">Cobalamin</keyword>
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    <keyword evidence="7" id="KW-0215">Deoxyribonucleotide synthesis</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="4 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="4"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001922"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007405"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ06362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="909" mass="103566" checksum="C12CA43F152F9D1A" modified="2017-09-27" version="1">MPVEKVMKRDGRIVPFDKERIRWAIQRAMLEVGVHDDKLLNKVVRRVVRRVNELYDGQVPNIENIQDIVELELMRAGLFDVAKAYILYRKRKAEIREEKKKILNKDKLDEIDKRFSINALRVLASRYLIKNEKGEIIESPRELFERVAVLAVIPDLLYDERVFDKNGGHAANPKGIEKYLSKLDEYDGKLSIGRFKLNKWHFERLLNLYRELAEKGQMKVSIDEIVEMLENGAFDKYEDEIEEYFRLMTGQIFMPNTPALINSGRPLGMLSACFVVPIEDDMESIMKAAHDVAMIQKMGGGTGLNFSKLRPEGDLVGTTTGAASGPVSFMHLIDAVSDVIKQGGVRRGANMGILEVWHPDIEKFVHAKERNTGTNVLSNFNISVGLWEDFWEALKEGKRYPLVNPRTGEKVKEIDPKSLFEELAYMAWAKADPGVVFFDVINRRNVLQSAKNEPIRATNPCGEEPLYDYESCNLASINLAKFVKYDDEGKPYFDWDEYAYVIQKVAKYLDNAIDVNKFPLPEIDRNTKLTRRIGVGMMGLADALFKLGIPYNSKEGFEFMRKATEYLTFYAYRQSVETAKKRGPFPLYEKTKYKDGELPVEGFYHREIWNLPWDELAEDIKRYGVRNGMVTTCPPTGSVSMIADTSSGIEPIFALVYKKSVTVGEFYYVDPVFEAELKRRGLYNDEILKKISDNYGSVQGLEEIPEELQRVFVTSMDIHWLDHLLAQANIQLWLTDSASKTINMPNDATVEDVKAAYLLAYKLGCKGVTVYRDGSLSVQVYSVEGEKRKRVPAKPSKYAIEKLKAVVEAEPWLARFINVEAILNGTNGKKESQPRGLTFPVSHISAPKPAHEHPHHAEKPEIPEEKIRELLGVAYCPVCYEKDGELVELRMESGCATCPRCGWSKCVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6G7</accession>
    <name>A0A218P6G7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03025</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853638.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6G7"/>
    <dbReference type="GeneID" id="33315211"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03025"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="115" mass="12909" checksum="224E61CCF000A65C" modified="2017-09-27" version="1">MQRGPTGWLVLKYFPEDVVVEVLSLARFALDLELAVETRKAFFVLKNGKIAGVIIFLSKKPPKEDRKFMAGLEEKLGVPVYAVFTKSSKYIAIDSEGEEIPLEQEELGAFVDLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6H0</accession>
    <name>A0A218P6H0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Polysaccharide biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_03065</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853645.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6H0"/>
    <dbReference type="GeneID" id="33315219"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03065"/>
    <dbReference type="OrthoDB" id="19148at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd13128">
        <property type="entry name" value="MATE_Wzx_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050833">
        <property type="entry name" value="Poly_Biosynth_Transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002797">
        <property type="entry name" value="Polysacc_synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30250:SF27">
        <property type="entry name" value="POLYSACCHARIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30250">
        <property type="entry name" value="PST FAMILY PREDICTED COLANIC ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01943">
        <property type="entry name" value="Polysacc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="336"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="397"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="431"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="455"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="509" mass="56949" checksum="E6D12E1B4068A280" modified="2017-09-27" version="1">MSEASQALQNIARGTGIVFAGTVISMFFGFLSRGVIARYFSTSEYGVFNLALTILSITLVLATLGFPNSLPREVAFYREKEPSRVRDLISTAVVIVAVNSFAIMMFLIFGSGFIAQVFNEERLVYPLKVMAFALPFSALIGAIISISQGFGRVREKVYFQNVVYPVIWLVFVVLLALLSLPFASLFWAYVIAQFLTFLTLILETYRIGLFRFRPSLDVGLGRKLVAFSFPLMFVGILNFLMTWTDTLMLGYYKGSEVVGLYNAATPLARLIPMFLNSAAVIYSPIVTSWYAQEKMAEMKRVYQVLTKWIFLLTLPLFALIFLFPEATISFLFGEKYVSAALALQVLSLGFMFHTFLGLNGMTLIVIGKPKLNMIGDTFAVVSNVALNLALIPSYGMVGAAVATTVSYFVANVFRSYWLYKMTRIHPFSWSYVKPLAIWFVLLGIIKGLNLSVPDIWYAILALLVFMGVYAVLVLLSRSIDKEDIELLLAVEKILGVDFGIIKRVLKRFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6H7</accession>
    <name>A0A218P6H7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Acetyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_269466916.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6H7"/>
    <dbReference type="GeneID" id="77169219"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03090"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03349">
        <property type="entry name" value="LbH_XAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050179">
        <property type="entry name" value="Trans_hexapeptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43300">
        <property type="entry name" value="ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43300:SF11">
        <property type="entry name" value="ACETYLTRANSFERASE RV3034C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="234" mass="26139" checksum="BFE4A7ABC17AE279" modified="2017-09-27" version="1">MSVKRFLKGLSAVVPYLIVKRSILIPPIYIDKVTVGKNVLIMQNTHIKNSTIADYVKIYANNTIWYSRIGEFSYINKNSTVVLAKIGRFCAIGPGVVIGAIKHTSNKISQYPAEMSKDLLVEVGSDVWIGANAVIMPGVRIGNGAIIGAGAVVTRDVPDYAIAVGVPAKIKKYRFTEEIIEKLKEIEWWNFPVEYLKNPEVRKVLFSDPTPENLKKLEEIKADIITTKKVDKYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6I2</accession>
    <name>A0A218P6I2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853639.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6I2"/>
    <dbReference type="GeneID" id="33315212"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03030"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="214" mass="24800" checksum="1840906A720457F5" modified="2017-09-27" version="1">MRRRTNHVLGGEVPSTVEYTARSNPLEADYYRQFPEIFDGHHVYIPDDILHQISRKERKAMRKIRRGKMLSAEEAETILSLIKKIRRHHHTLELSDEEVLEHISIITQHVLNANRKNPGPWGETSFYNMRRIVRAYAEMKCLNLATLFEEALSRKLISLEEFSGLVYNLMNARKNLRAGGRVRLVDGSYITLRGTEPFFHWAETSISGVKEAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6J3</accession>
    <name>A0A218P6J3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_03160</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853658.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6J3"/>
    <dbReference type="GeneID" id="33315238"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03160"/>
    <dbReference type="OrthoDB" id="85636at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45641:SF19">
        <property type="entry name" value="NEPHROCYSTIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45641">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_6G03870)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="55"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="93"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="113"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="341" mass="39488" checksum="70B740380749B7F6" modified="2017-09-27" version="1">MTDVKAEWENALKNKDCEKLLELFDDYVDTIDDEETLRRELERLKSVALECEDPYDLAHEIGHVYAHLDDVEGGIELYKRLVELKKDDPEEYATALYYLADAYEHFGKPEKAIEVYEKLLKHEEEVLKNEKEIALTLANLAVNYDELGETEKAIELMERAREIFEKLNDEKNLMISLLDLAHFHYELGNYDTAETLIKEVLRNPRDDEIEVNAKLVEAEIWAGREDYDKAFKALREALLKAINVSDDVFGVVFDTLVDFIEGLFNEGAYEAVAKNMDAFAELFEDDTAYFFKAIAELARWKAGDEDATKRFDELYSKVENEELKAILDEWKRPKLGLSLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6P9</accession>
    <name>A0A218P6P9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nitrosopumilus output domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03515</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853720.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6P9"/>
    <dbReference type="GeneID" id="33315308"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03515"/>
    <dbReference type="OrthoDB" id="95069at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.200">
        <property type="entry name" value="Protein of unknown function DUF3227"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021609">
        <property type="entry name" value="NitrOD5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044908">
        <property type="entry name" value="NitrOD5-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11537">
        <property type="entry name" value="NitrOD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitrosopumilus output" evidence="1">
        <location>
            <begin position="8"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="100" mass="11257" checksum="ABFE1A3F4D338037" modified="2017-09-27" version="1">MSEFHSEINRGMVVATARELLTKFGPHFLVAVEAYLKAKYGETLELAGRDPELFYDAVKDLFGEFAAVMFLQSLVRELHLSVEEESEEGLLKALKSYVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6V5</accession>
    <name>A0A218P6V5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03685</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853748.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6V5"/>
    <dbReference type="GeneID" id="33315342"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03685"/>
    <dbReference type="OrthoDB" id="102549at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.210">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="3"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="120"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="263"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="353" mass="42488" checksum="B63298BFB6C2F201" modified="2017-09-27" version="1">MKLEQALDEYERKREKALKEAEKIRKNYNKRLEKKVREILKRIDKLDRKDIPKNVDDSIKKIVTAERRSYVTALRNALSSVNDMESLGKRLPDLAKLHVGHGRYLLIIFEKDVYAINRLLKELNEEYLNYHNELAEKGLEDLRIREKMREIEDVKKDIERVKDELTELTERLRTGERELEEFYDREGLPKLEEEIKRLSSKVKNEELEVRSKVSKLQKPVKRMRLHEPIAEELVRDSSVALGRPKEFIEFVRKIEPKLEPKQRKAAKWLLENLEERAEEIRARRKELRELEVRREEILSRGHELEEELKRLKRLIEEREAELKKLGNRLEHLESELEKEIERLEKILGTEIER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P731</accession>
    <name>A0A218P731_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein eL34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">rpl34e</name>
        <name evidence="7" type="ORF">A3L08_04180</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL34 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853840.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P731"/>
    <dbReference type="GeneID" id="33315440"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04180"/>
    <dbReference type="OrthoDB" id="43096at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00349">
        <property type="entry name" value="Ribosomal_eL34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008195">
        <property type="entry name" value="Ribosomal_eL34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038562">
        <property type="entry name" value="Ribosomal_eL34_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018065">
        <property type="entry name" value="Ribosomal_eL34_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047868">
        <property type="entry name" value="Ribosomal_L34e_arc-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003143">
        <property type="entry name" value="PRK04059.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10759">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01199">
        <property type="entry name" value="Ribosomal_L34e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01250">
        <property type="entry name" value="RIBOSOMALL34"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01145">
        <property type="entry name" value="RIBOSOMAL_L34E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="50"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="90" mass="10428" checksum="0B2FE831C16E409A" modified="2017-09-27" version="1">MKPMYRSRSWKRKYVRTPGGRTVIHFERRKPKVAHCAMCGRPLNGVPRGRPSELRKLPKTAKRPERPYPNLCPSCMRKVMKAQVRAGIAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P740</accession>
    <name>A0A218P740_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">AsnC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_04330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853862.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P740"/>
    <dbReference type="GeneID" id="33315470"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04330"/>
    <dbReference type="OrthoDB" id="8136at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="1">
        <location>
            <begin position="6"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="79" mass="9313" checksum="FB74BE72D4C87A76" modified="2017-09-27" version="1">MMEVFVLVVVKPGHEEEVYNALSGHERIKEIYRVYGEYDLILRVEVENVEALDRFHDEVLRKLKNIEMTETLIASSYRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P743</accession>
    <name>A0A218P743_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Large ribosomal subunit protein eL40</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpl40e</name>
        <name evidence="5" type="ORF">A3L08_04285</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eL40 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853854.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P743"/>
    <dbReference type="GeneID" id="33315461"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04285"/>
    <dbReference type="OrthoDB" id="45138at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00788">
        <property type="entry name" value="Ribosomal_eL40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023657">
        <property type="entry name" value="Ribosomal_eL40_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001975">
        <property type="entry name" value="Ribosomal_eL40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038587">
        <property type="entry name" value="Ribosomal_eL40_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003161">
        <property type="entry name" value="PRK04136.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39649">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L40E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39649:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01377">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL40" evidence="4">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="52" mass="6034" checksum="E7A7732AACCA28BC" modified="2017-09-27" version="1">MARFPEAEARIFRKYICMRCGATNPWKAEKCRKCGYKGLRPKAREPRGGMGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P750</accession>
    <name>A0A218P750_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">AsnC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_04310</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853858.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P750"/>
    <dbReference type="GeneID" id="33315466"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04310"/>
    <dbReference type="OrthoDB" id="6995at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154">
        <property type="entry name" value="LEUCINE-RESPONSIVE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR AZLB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="149" mass="17235" checksum="7E76AE768F6A76CE" modified="2017-09-27" version="1">MDELDLKILSLLQENARLSYREIARELRVAVGTVYNRIKRMEEEGVIRGFAPVLDYEKLGFGLTAVIGVKAQGRRIVEIEREIARNDRVMLVYDITGEFDIVVVAKFRDRADMNRFVKWLLSLEGVEKTNTSVAMQVVKEDMRLRLTED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P754</accession>
    <name>A0A218P754_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable 6-oxopurine nucleoside phosphorylase</fullName>
            <ecNumber evidence="5">2.4.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Purine nucleoside phosphorylase</fullName>
            <shortName evidence="5">PNP</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_04350</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Purine nucleoside phosphorylase which is highly specific for 6-oxopurine nucleosides. Cleaves guanosine or inosine to respective bases and sugar-1-phosphate molecules. Involved in purine salvage.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a purine D-ribonucleoside + phosphate = a purine nucleobase + alpha-D-ribose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:19805"/>
            <dbReference type="ChEBI" id="CHEBI:26386"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="ChEBI" id="CHEBI:142355"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Purine metabolism; purine nucleoside salvage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 5">Homohexamer. Dimer of a homotrimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="5">Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.1" evidence="5"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853866.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P754"/>
    <dbReference type="GeneID" id="33315474"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04350"/>
    <dbReference type="OrthoDB" id="7681at2157"/>
    <dbReference type="UniPathway" id="UPA00606"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017061">
        <property type="term" value="F:S-methyl-5-thioadenosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09010">
        <property type="entry name" value="MTAP_SsMTAPII_like_MTIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1580:FF:000012">
        <property type="entry name" value="Probable 6-oxopurine nucleoside phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01963">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010044">
        <property type="entry name" value="MTAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018099">
        <property type="entry name" value="Purine_phosphorylase-2_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01694">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006599">
        <property type="entry name" value="PRK09136.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679">
        <property type="entry name" value="S-METHYL-5'-THIOADENOSINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679:SF2">
        <property type="entry name" value="S-METHYL-5'-THIOADENOSINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01240">
        <property type="entry name" value="PNP_MTAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3 5" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="6">
        <location>
            <begin position="3"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="50"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="83"/>
            <end position="84"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="212"/>
            <end position="214"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="5">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="5">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00063054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="267" mass="29759" checksum="96E60A732A965036" modified="2017-09-27" version="1">MPRIAIIGGSGVYDPKLLENVREEFVSTPYGKVRVKVGEYDGEEIAFLARHGEGHSVPPHKINYRANIWALHELGVERILSTSAVGSLNEAMKPGDFVILDQLMDFTKTRHYTFYDGDESPHDRKFVAHVDFTDPYCPELRKALITAAKELGFSYHPTGTYACMEGPRFETRAEIRALKILGADVVGMTQCPEAALARELEMCYASVAIVTNFAAGISTQKLTHTEVVELMAQKSEEIKYLLMKSIKYIPRERHCGCKDALKGATGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P771</accession>
    <name>A0A218P771_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_04510</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853891.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P771"/>
    <dbReference type="GeneID" id="33315506"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04510"/>
    <dbReference type="OrthoDB" id="10960at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30115">
        <property type="entry name" value="NITROGEN REGULATORY PROTEIN P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30115:SF11">
        <property type="entry name" value="NITROGEN REGULATORY PROTEIN P-II HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00340">
        <property type="entry name" value="PIIGLNB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00938">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="GlnB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51343">
        <property type="entry name" value="PII_GLNB_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="105" mass="11548" checksum="06FD933C05BE072C" modified="2017-09-27" version="1">MKKVEAILRPEDFENVKKALKSAGFMPLTVSSVQGRGVQGGVPPYDLLPKIKLEIVVNDSDLEQVIDIIARNAKRGIPGDGKIFVLPVYDAVRIRTGERGEEALY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P778</accession>
    <name>A0A218P778_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha-glucosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_04515</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853892.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P778"/>
    <dbReference type="GeneID" id="33315507"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04515"/>
    <dbReference type="OrthoDB" id="85762at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="214"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="32"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="244" mass="27711" checksum="045CF797AED90EA0" modified="2017-09-27" version="1">MKSRDILLDTAEVLESTLGRIDRITLLTDKEKERVKAKISEASKNFKKLAEEVEKDNEELAEFFYKKAKEFKTMSTDKAIEREGKKNYLKLADRILLYSRSAEYDFVPEKMKELKGVYRKYLFGMTAFFMLTGFYLNQFLAITALILAIPIVLSMLSLQRRGYLGLLLAYSAIPIPVIVGAMALSYGMRNLNDPAKIAEIAQHLSKSTTFAKGYLIVLVLLAAVELYLVLSSAVGLYKHRHAFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7A3</accession>
    <name>A0A218P7A3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_04655</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853918.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7A3"/>
    <dbReference type="GeneID" id="33315535"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04655"/>
    <dbReference type="OrthoDB" id="41163at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="2">
        <location>
            <begin position="38"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="176" mass="20265" checksum="8AA75F0161542CC4" modified="2017-09-27" version="1">MDEKTLRKGERYYKSGKVLWVVKYGDRLFSKVLGTYPYYVELDLRTGENRCTCPLGGDCKHVAAVMKAHESGFYFETFDRHAELFPEAVAMEFLAEVPELALDVILKELRFALSTDESGSEVARLLRRALKLTEATGKREALHFLEDAVEEYKHVFSDYELSLKLEDELRELKTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7B1</accession>
    <name>A0A218P7B1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_04660</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853919.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7B1"/>
    <dbReference type="GeneID" id="33315536"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04660"/>
    <dbReference type="OrthoDB" id="103573at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="77" mass="8831" checksum="E142B848C4BAD3C5" modified="2017-09-27" version="1">MDVFELARKYHDELGIKEPSMATMAAEFFDDLGLKMAEFLKGEGYTIVGTKFIDYDKSLVIDTTKGEKRFEITLRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7B5</accession>
    <name>A0A218P7B5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sodium:dicarboxylate symporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_04725</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853931.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7B5"/>
    <dbReference type="GeneID" id="33315549"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04725"/>
    <dbReference type="OrthoDB" id="3015at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3860.10">
        <property type="entry name" value="Sodium:dicarboxylate symporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001991">
        <property type="entry name" value="Na-dicarboxylate_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036458">
        <property type="entry name" value="Na:dicarbo_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42865">
        <property type="entry name" value="PROTON/GLUTAMATE-ASPARTATE SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42865:SF7">
        <property type="entry name" value="PROTON_GLUTAMATE-ASPARTATE SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00375">
        <property type="entry name" value="SDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00173">
        <property type="entry name" value="EDTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118215">
        <property type="entry name" value="Proton glutamate symport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="244"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="316"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="343"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="377"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="434" mass="46638" checksum="E2E57C0D5A342D70" modified="2017-09-27" version="1">MSILKRYISIPLPYRVGVAFLLGVTFGLVLWYYDMANGSNLGQGVATYVSPFGAVLVRMLKMVVIPIVFFSLIVGSSGLSPKKLGRVGGKVILWYMLTSFLAAVIGVGIAMFVHPGQGVAVDVEKLGSSMEEGGPSITPPSGLTDLILKMFTNPFEALANGEFLPIIVFALLFGLAARVLLDHTQNETERKQIQSILDFASGANSVMFKIVSWILEYFPIGVFSLAVVNFGLYGPSIIGPYLKAVLGVISGIAIMVFIIYPILLSLILKENPIDFFKRAKEVMITSFFTRSSAATLPVTIKTAIESFKIKKELAEFSLPVGATINMNGVCIHLPMWAVFATDVFNIDLTVSSLVIIIITTVLSAIGTGGVPGGSLMLLFIVLGTLGLEPNQIATIVALALGVNPLIDMFETMNNVTGDLCCTYIVAKKEGMIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7E2</accession>
    <name>A0A218P7E2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Histone</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_04845</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal histone HMF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853948.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7E2"/>
    <dbReference type="GeneID" id="33315573"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04845"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22909">
        <property type="entry name" value="HFD_archaea_histone-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050947">
        <property type="entry name" value="Archaeal_histone_HMF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050004">
        <property type="entry name" value="HmfB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF043032">
        <property type="entry name" value="archaea_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47828">
        <property type="entry name" value="ARCHAEAL HISTONE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47828:SF1">
        <property type="entry name" value="ARCHAEAL HISTONE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="7">
        <location>
            <begin position="2"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="67" mass="7420" checksum="10C60492E68ABF74" modified="2017-09-27" version="1">MAELPIAPIDRLIRKAGAERVSEEAAKVLAEYLEEYAIELAKKANDFARHAGRKTIKAEDIKLAIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7G7</accession>
    <name>A0A218P7G7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable glycine dehydrogenase (decarboxylating) subunit 2</fullName>
            <ecNumber evidence="5">1.4.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Glycine cleavage system P-protein subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Glycine decarboxylase subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Glycine dehydrogenase (aminomethyl-transferring) subunit 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">gcvPB</name>
        <name evidence="8" type="ORF">A3L08_05005</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[glycine-cleavage complex H protein] + glycine + H(+) = N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[glycine-cleavage complex H protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:24304"/>
            <dbReference type="Rhea" id="RHEA-COMP:10494"/>
            <dbReference type="Rhea" id="RHEA-COMP:10495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="1.4.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">The glycine cleavage system is composed of four proteins: P, T, L and H. In this organism, the P 'protein' is a heterodimer of two subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the GcvP family. C-terminal subunit subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.4.4.2" evidence="5"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853976.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7G7"/>
    <dbReference type="GeneID" id="33315605"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05005"/>
    <dbReference type="OrthoDB" id="371967at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016594">
        <property type="term" value="F:glycine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004375">
        <property type="term" value="F:glycine dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00613">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000034">
        <property type="entry name" value="Probable glycine dehydrogenase (decarboxylating) subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000014">
        <property type="entry name" value="Probable glycine dehydrogenase (decarboxylating) subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00713">
        <property type="entry name" value="GcvPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023012">
        <property type="entry name" value="GcvPB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049316">
        <property type="entry name" value="GDC-P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049315">
        <property type="entry name" value="GDC-P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020581">
        <property type="entry name" value="GDC_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003346">
        <property type="entry name" value="PRK04366.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773:SF1">
        <property type="entry name" value="GLYCINE DEHYDROGENASE (DECARBOXYLATING), MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773">
        <property type="entry name" value="GLYCINE DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21478">
        <property type="entry name" value="GcvP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02347">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycine cleavage system P-protein N-terminal" evidence="6">
        <location>
            <begin position="33"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine dehydrogenase C-terminal" evidence="7">
        <location>
            <begin position="355"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="5">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF21478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="502" mass="56145" checksum="113059FDC6C2638A" modified="2017-09-27" version="1">MFRQAKWDEPTIFELSRPGRIGYTLPKPIEDVEVEIPEKLRRKSPLNLPELSEPEVVKHYTRLSEMNYGVDSGIYPLGSCTMKYNPKINEEIASHPGVAYVHPYQDERTVQGALRIMWELEQWLKEITGMDRFTLQPAAGANGEFTGVSIIRAYHLDRGEVQRDEMLVPDSAHGTNPASAAMAGFKVIEIPSNENGTVDLEALENAVSERTAGLMLTNPNTLGIFEDEILEIAKIVHKAGGLLYYDGANLNAVLGKVRPGDMGFDVVHLNLHKTFSTPHGGGGPGSGPVGVKDFLKDYLPVPLVSYDEENDRYYLDYNVPKSIGKVKELYGNFAVIVRALTYLKIMGRDGLREVSEVAVLNANYLTQKLKGTRGYELPGKELRKHETVFSAEPMKKETSVTAMDVAKRLLDFGMHAPTVYFPLIVHEALMIEPTETVTKEELDAYVEALKRISEEAYTNPEVVKSAPHNTAVKRVDDVLAAKRPVITWRMYRELKEKGEIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7H0</accession>
    <name>A0A218P7H0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein eL30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl30e</name>
        <name evidence="7" type="ORF">A3L08_05050</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853981.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7H0"/>
    <dbReference type="GeneID" id="33315614"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05050"/>
    <dbReference type="OrthoDB" id="10759at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000053">
        <property type="entry name" value="50S ribosomal protein L30e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00481">
        <property type="entry name" value="Ribosomal_eL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000231">
        <property type="entry name" value="Ribosomal_eL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039109">
        <property type="entry name" value="Ribosomal_eL30-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022991">
        <property type="entry name" value="Ribosomal_eL30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002172">
        <property type="entry name" value="PRK01018.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11449">
        <property type="entry name" value="RIBOSOMAL PROTEIN L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00709">
        <property type="entry name" value="RIBOSOMAL_L30E_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00993">
        <property type="entry name" value="RIBOSOMAL_L30E_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="6">
        <location>
            <begin position="7"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="101" mass="10936" checksum="65A48F02720EDA33" modified="2017-09-27" version="1">MVDFAFELRKAEETGKIVMGAKKAIQYAKMGGAKLIIVARNARPDLKEDILYYAKLSGIPIYEFEGTSVELGTLLGRPHTVSALAILDPGESRILALGGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7I3</accession>
    <name>A0A218P7I3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lysine exporter LysO family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853992.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7I3"/>
    <dbReference type="GeneID" id="33315629"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05125"/>
    <dbReference type="OrthoDB" id="21422at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015661">
        <property type="term" value="F:L-lysine efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005642">
        <property type="entry name" value="LysO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35804">
        <property type="entry name" value="LYSINE EXPORTER LYSO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35804:SF1">
        <property type="entry name" value="LYSINE EXPORTER LYSO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03956">
        <property type="entry name" value="Lys_export"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="20538" checksum="D1CDD71FEEB40CB3" modified="2017-09-27" version="1">MRFLAYVLASLIAGMAVGHFYAPDFGNLYEVMLYLLILIIGIDLGGSFRLGEIKKLGKLAVKLPLGTLIGSLIGGLVASLLLGIELKWGLAVSAGCGWYSLTGPLIGQYSAVYGTLGFLANLMREILTVLFYPLAIKRLPKELAVSMGGATTMDTTLPLLTKFGGSEVALIAFVHGFVLTALVPFVVPFILQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7K3</accession>
    <name>A0A218P7K3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05255</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7K3"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05255"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="59" mass="7079" checksum="99706C768D9E3920" modified="2017-09-27" version="1">MTGFFFFFILSKAGELLTPEKLWKLKVRKIGIDLVTGKFSRNERAYAQSLYLKRYSWVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7K6</accession>
    <name>A0A218P7K6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Molybdopterin synthase sulfur carrier subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854022.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7K6"/>
    <dbReference type="GeneID" id="33315662"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05290"/>
    <dbReference type="OrthoDB" id="98357at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd17505">
        <property type="entry name" value="Ubl_SAMP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010038">
        <property type="entry name" value="MoaD_arc-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052045">
        <property type="entry name" value="Sulfur_Carrier/Prot_Modifier"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01687">
        <property type="entry name" value="moaD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38031:SF1">
        <property type="entry name" value="SULFUR CARRIER PROTEIN CYSO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38031">
        <property type="entry name" value="SULFUR CARRIER PROTEIN SLR0821-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="82" mass="9025" checksum="A84A09729233428C" modified="2017-09-27" version="1">MLVKLFATLIEFTGKRKLEISGPKTVGELLEELDRMFPGFKKELEKGRIILVNGKNIEHLQGLDTPLSDEDTVSIFPPAGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7L6</accession>
    <name>A0A218P7L6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05315</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854027.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7L6"/>
    <dbReference type="GeneID" id="33315667"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05315"/>
    <dbReference type="OrthoDB" id="359241at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00748">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81593">
        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="7"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="121" mass="13953" checksum="8F63A75A64261153" modified="2017-09-27" version="1">MSYERWISKGEDDIRLAELALENGIYDYAAFHAQQAVEKFLKAFLVKAGKPVPRTHDIAYLIELCKEVDPSFERLYDMNAHYLSDFAVEVRYPGYYTVPKEIAAEAIETAKKVLEFVLDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7M6</accession>
    <name>A0A218P7M6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Carbamoyltransferase</fullName>
            <ecNumber evidence="8">6.2.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">A3L08_05350</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>C-terminal L-cysteinyl-[HypE protein] + carbamoyl phosphate + ATP + H2O = C-terminal S-carboxamide-L-cysteinyl-[HypE protein] + AMP + phosphate + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55636"/>
            <dbReference type="Rhea" id="RHEA-COMP:14247"/>
            <dbReference type="Rhea" id="RHEA-COMP:14392"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:76913"/>
            <dbReference type="ChEBI" id="CHEBI:139126"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>an acyl phosphate + H2O = a carboxylate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="3.6.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; [NiFe] hydrogenase maturation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the carbamoyltransferase HypF family.</text>
    </comment>
    <dbReference type="EC" id="6.2.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854034.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7M6"/>
    <dbReference type="GeneID" id="33315674"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05350"/>
    <dbReference type="OrthoDB" id="371970at2157"/>
    <dbReference type="UniPathway" id="UPA00335"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003998">
        <property type="term" value="F:acylphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016743">
        <property type="term" value="F:carboxyl- or carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000124">
        <property type="entry name" value="Carbamoyltransferase HypF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001792">
        <property type="entry name" value="Acylphosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036046">
        <property type="entry name" value="Acylphosphatase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051060">
        <property type="entry name" value="Carbamoyltrans_HypF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004421">
        <property type="entry name" value="Carbamoyltransferase_HypF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041440">
        <property type="entry name" value="HypF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055128">
        <property type="entry name" value="HypF_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="Sua5-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011125">
        <property type="entry name" value="Znf_HypF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00143">
        <property type="entry name" value="hypF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42959">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42959:SF1">
        <property type="entry name" value="CARBAMOYLTRANSFERASE HYPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00708">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17788">
        <property type="entry name" value="HypF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22521">
        <property type="entry name" value="HypF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07503">
        <property type="entry name" value="zf-HYPF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006256">
        <property type="entry name" value="CMPcnvr_hdrg_mat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54975">
        <property type="entry name" value="Acylphosphatase/BLUF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51160">
        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Acylphosphatase-like" evidence="10">
        <location>
            <begin position="3"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="YrdC-like" evidence="11">
        <location>
            <begin position="200"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="772" mass="86607" checksum="0F6F37A97216017B" modified="2017-09-27" version="1">MKAYHIHVQGIVQAVGFRPFVYRIAHEHNLKGYVKNLGDAGVEIVVEGREEDIEAFLRDLHRKKPPLARIDRVEKREIPPQGFPEFYIERSSKGGKGGDSIIPPDIAICDDCLRELFDPTDKRYMYPFIVCTNCGPRFTIIEDLPYDRENTTMREFPMCDFCRSEYEDPLNRRYHAEPTACPVCGPSYRLYTSDGKELTGDPLRKAAELIDKGYIVAIKGIGGIHLACDATNEEAVVELRRRTFRPQKPFAIMADSLETVKSFAYVSREEEEELTSYRRPIITLRKKEPFPLPENLAPGLHTIGVMLPYAGTHYILFHWSRSKVYVMTSANYPGMPMVKDNERAFEELKEMADYLLLHNRKILNRADDSVIRFVDGKRAVIRRSRGFVPLPVEIPFDYRGLAVGAELMNAFGVSKNGKVYPSQYIGNTGKLEVLEFMREAIAHFRKILRVKDFDLIISDLHPGYNTTKLAMELANELDVELLQVQHHYAHIASVLAEKNLESGVGIALDGVGYGTDGNVWGGEVLYLGYEDVERLAHIDYYPLPGGDLASYYPLRALMGILSKVYSIEELGEIIGRCCPKAVESLKYGKVEFSVILNQLAKGVNTAYASSTGRVLDSLAVLLNVAYRRHYEGEPAMKLESFAMKGKNDLKFEVPVEGELIKVEELFLQALDAIEKASPADIAYSVHLALARAFANVAIEKAMEFGVKDVAISGGVAYNELIVKTIRKAVEASGLRFHATTEVPRGDNGINVGQAFLGGLYLEGYLSKEDLML</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7N9</accession>
    <name>A0A218P7N9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Tungsten cofactor oxidoreducase radical SAM maturase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_05285</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854021.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7N9"/>
    <dbReference type="GeneID" id="33315661"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05285"/>
    <dbReference type="OrthoDB" id="5620at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21121">
        <property type="entry name" value="SPASM_Cmo-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034391">
        <property type="entry name" value="AdoMet-like_SPASM_containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017200">
        <property type="entry name" value="PqqE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050377">
        <property type="entry name" value="Radical_SAM_PqqE_MftC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027604">
        <property type="entry name" value="W_rSAM_matur"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04317">
        <property type="entry name" value="W_rSAM_matur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228:SF34">
        <property type="entry name" value="TUNGSTEN-CONTAINING ALDEHYDE FERREDOXIN OXIDOREDUCTASE COFACTOR MODIFYING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13186">
        <property type="entry name" value="SPASM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037420">
        <property type="entry name" value="PQQ_syn_pqqE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01387">
        <property type="entry name" value="BtrN-like_SPASM_domain_contain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01386">
        <property type="entry name" value="main_SPASM_domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00570">
        <property type="entry name" value="tungsten_cofactor_oxidoreducas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="35"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="393" mass="45819" checksum="843680E73D075188" modified="2017-09-27" version="1">MKIPLAYSRRQGAVELTDKEYTFSLWDGKVIVRPKLDMKYLYIEITSRCNLKCEMCFKQYWEDEEGDMDWELFLKILDDAEEFPELKMIYFGGIGEPTVHPRFMDMVREVKKRGFALGMSSNGTLMTDDMLREFAKLGVDLIYFSMDTVPTAQNAITLGHLAAAATADRIKRLVKYRDEYGTHRPSVGVEVVVTKENYKQLPEMARFLLDLGVDSMLVSNLLPMTGEQMEDIIYDGSVDMKPILEELYRIAHNGLYIKLPYFELKTERSCDFDENNVAVVRWDGEVAPCYRFLHSYYEYIFGRKKKVNAYSFGNVREKSLAEIWTSEKYTWFRFTMKNYMYPSCTDCPLVDACDFVKTSDIDCWGNEPSCADCLWARRIVQCPIPQHNFGKFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7P8</accession>
    <name>A0A218P7P8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">CTP synthase</fullName>
            <ecNumber evidence="11">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cytidine 5'-triphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Cytidine triphosphate synthetase</fullName>
            <shortName evidence="11">CTP synthetase</shortName>
            <shortName evidence="11">CTPS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 14" type="primary">pyrG</name>
        <name evidence="14" type="ORF">A3L08_05455</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>UTP + L-glutamine + ATP + H2O = CTP + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>UTP + NH4(+) + ATP = CTP + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:16597"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="11">Allosterically activated by GTP, when glutamine is the substrate; GTP has no effect on the reaction when ammonia is the substrate. The allosteric effector GTP functions by stabilizing the protein conformation that binds the tetrahedral intermediate(s) formed during glutamine hydrolysis. Inhibited by the product CTP, via allosteric rather than competitive inhibition.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homotetramer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="11">CTPSs have evolved a hybrid strategy for distinguishing between UTP and CTP. The overlapping regions of the product feedback inhibitory and substrate sites recognize a common feature in both compounds, the triphosphate moiety. To differentiate isosteric substrate and product pyrimidine rings, an additional pocket far from the expected kinase/ligase catalytic site, specifically recognizes the cytosine and ribose portions of the product inhibitor.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the CTP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854054.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7P8"/>
    <dbReference type="GeneID" id="33315695"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05455"/>
    <dbReference type="OrthoDB" id="52769at2157"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097268">
        <property type="term" value="C:cytoophidium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000009">
        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000002">
        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01227">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003792">
        <property type="entry name" value="PRK05380.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00337">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="CTP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550:SF0">
        <property type="entry name" value="CTP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTP synthase N-terminal" evidence="13">
        <location>
            <begin position="3"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase" evidence="12">
        <location>
            <begin position="300"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Amidoligase domain" evidence="11">
        <location>
            <begin position="1"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for glutamine hydrolysis" evidence="11">
        <location>
            <position position="378"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="505"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="507"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="14"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="146"/>
            <end position="148"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="186"/>
            <end position="191"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="186"/>
            <end position="191"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="379"/>
            <end position="382"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="459"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF06418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ06803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="533" mass="59929" checksum="67F3B51821BB8AF6" modified="2017-09-27" version="1">MAKFIFVTGGVVSGLGKGITSASLGMLMKARGFRTTNIKIDPYINYDAGTMNPYQHGEVFVLDDGGEVDLDLGNYERFLDTSLSFDHNITTGKVYSAVIEKERKGEYLGATVQVIPHITNEIKERIRRIARDYDVVVVEIGGTVGDIESMPFLEAARQMQIEEGRENVAFVHVTYVPKLRVVGEQKTKPTQHSVKELRSLGIQPDAIVARSEEPLEESARLKISLFTNVPDEAVISAYDVEDTYEVPLMLEKEGLAKYLTKRLGLPEKEPELEAWREMVEKYKNLEDTVEIAIVGKYVKLADSYLSIKEALKHSSVANGVKVKIRWIEAEELEKAGTKLLEGVDGIIVPGGFGARGTEGKMMAVRYARENDIPFLGICFGFQLTVVEFARNVLGLKGAHSTEIDPQTPYPVVDLMPEQRDLDRLGGTMRLGAYPVHIKPNTLARELYGREIVYERHRHRWEVNPEYIEQFEKAGLVFSGIAGDDGRRMEILELPEHSYFIATQFHPEFKSRPMNPAPVFRGLVKAVKEKKYES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7P9</accession>
    <name>A0A218P7P9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF424 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854043.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7P9"/>
    <dbReference type="GeneID" id="33315683"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05395"/>
    <dbReference type="OrthoDB" id="18015at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1860.10">
        <property type="entry name" value="uncharacterized conserved protein from methanopyrus kandleri domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007355">
        <property type="entry name" value="DUF424"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04242">
        <property type="entry name" value="DUF424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="99" mass="11338" checksum="D4FECD1422CCEC94" modified="2017-09-27" version="1">MIYVKIYRVQGEVLLAACDEELLGKTFREGELKLEVKERFYKGELVEEDALENLLEEATIANLTGERCVSKAIELGYVDEERVLYIDGVPHAQMARLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7T9</accession>
    <name>A0A218P7T9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05760</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7T9"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05760"/>
    <dbReference type="OrthoDB" id="86129at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="273" mass="31006" checksum="C192018C082DBDAC" modified="2017-09-27" version="1">MDEIEVIFYIEGLGNDKKVLESALKETAEKLRRDKSLEIVDLKMEDVIENSETETLKYSGMIEARLKGTLEAITIAAIKYAPAVVDIISPGKIEITAEELMKVLGKVSYMMGELMEKFGPLAAYPKFDELPEPKIGYTRDEIEEFIFNERMIVYRFVVEAYGNDEEEVKIGLAKGLEYEGCKINKLVTKVQEENRGRKYILIALELVSSFETLFQLTAKYAPVAISIIEPEIIDVTAYELQGALVDLAGFAYELTTRPLKEKIIEKETFKFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7V5</accession>
    <name>A0A218P7V5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thiamine-monophosphate kinase</fullName>
            <shortName evidence="1">TMP kinase</shortName>
            <shortName evidence="1">Thiamine-phosphate kinase</shortName>
            <ecNumber evidence="1">2.7.4.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thiL</name>
        <name evidence="4" type="ORF">A3L08_05785</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent phosphorylation of thiamine-monophosphate (TMP) to form thiamine-pyrophosphate (TPP), the active form of vitamin B1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>thiamine phosphate + ATP = thiamine diphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:15913"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine phosphate: step 1/1.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Reaction mechanism of ThiL seems to utilize a direct, inline transfer of the gamma-phosphate of ATP to TMP rather than a phosphorylated enzyme intermediate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiamine-monophosphate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.16" evidence="1"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854116.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7V5"/>
    <dbReference type="GeneID" id="33315761"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05785"/>
    <dbReference type="OrthoDB" id="45909at2157"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00142"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009030">
        <property type="term" value="F:thiamine-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02194">
        <property type="entry name" value="ThiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02128">
        <property type="entry name" value="TMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006283">
        <property type="entry name" value="ThiL-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004353">
        <property type="entry name" value="PRK05731.2-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01379">
        <property type="entry name" value="thiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270">
        <property type="entry name" value="THIAMINE-MONOPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270:SF3">
        <property type="entry name" value="THIAMINE-MONOPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005303">
        <property type="entry name" value="Thiam_monoph_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="2">
        <location>
            <begin position="23"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="3">
        <location>
            <begin position="145"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="322" mass="34863" checksum="2E68E0AD958F34E2" modified="2017-09-27" version="1">MRESEIIELFLKHLRKQGDLPLGDDAGAIRLGDEWLVATNDMLVRKTDVPDIMTPEQVGFKAVTMNVSDVAAMGARPIGFLFSLGVPADVEGSYLEGIAEGIGKALEFYNVPVISADTNEADDLIIDGIALGKTRKLLTRSGAGAGELVCVTGDIGRALAGLLAWKRNLEISERTKKALYEKLLEPRARVEEGVELSGVASAAVDISDGLSKELHLLAEMSGVGIEVDAEKLPIRREVFEAAELLGVDPVEIALASGEEFEIVFTVPERVLKGLKIDCTAIGRTFEGSGVYITTGEKRQEMPLLGWEHLEKGVKGKYRTLFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Y6</accession>
    <name>A0A218P7Y6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HEPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854157.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Y6"/>
    <dbReference type="GeneID" id="33315804"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06000"/>
    <dbReference type="OrthoDB" id="101012at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="7"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="71" mass="8072" checksum="A7E7B44B3F70E90D" modified="2017-09-27" version="1">MDDEISLLLSNSHESLGAAELLLEKGFFAESLRERADYDATFQPGEEEARELVEDAKAFVERIEKALEELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Z2</accession>
    <name>A0A218P7Z2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glyoxalase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05955</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854150.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Z2"/>
    <dbReference type="GeneID" id="33315795"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05955"/>
    <dbReference type="OrthoDB" id="197151at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07721">
        <property type="entry name" value="yflN-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050855">
        <property type="entry name" value="NDM-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951:SF17">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="16"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="201" mass="22333" checksum="5EC41B4033E13A90" modified="2017-09-27" version="1">MHEEVLPGIHRIFDKFVNVYLVEGGDHLVAVDTGVDTTCGKILDAVEEIGKPLTAIVLTHGHLDHTGSLKCLKERTNAIVAAHVDEVELIKEKTGLEPDVKLKDGDVFEDFRVLHKPGHTMGSICLLDEKSKSLFVGDLVVERGGKLEEVPHQYSLDPEMNRIRIKELLEVDFENLLPAHGEPLLGNGKEKLRELVERLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P812</accession>
    <name>A0A218P812_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRISPR-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854172.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P812"/>
    <dbReference type="GeneID" id="33315821"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06090"/>
    <dbReference type="OrthoDB" id="105865at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="18582" checksum="141DC6BF11711C87" modified="2017-09-27" version="1">MTKVAYITLLGRFPWAVVNTYYKLLTMGKKADRIYVFTEERYKDNLPKVVKALETISKAYNLDPIIETEVVPNYGFFVADKKFRELFSRLEREGYRIGLDITSGRKALVAAAIVQVRKFPVEFIVYMGLLDPDFPDRPYMMIPTHMQPIKNFLGDGDVGNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P848</accession>
    <name>A0A218P848_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Molybdenum cofactor biosynthesis protein MoaA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_06240</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854198.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P848"/>
    <dbReference type="GeneID" id="33315851"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06240"/>
    <dbReference type="OrthoDB" id="371936at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040088">
        <property type="entry name" value="MJ0103-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050377">
        <property type="entry name" value="Radical_SAM_PqqE_MftC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228:SF35">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01110">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="109"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="5">
        <location>
            <begin position="359"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="419" mass="47499" checksum="490C456C18B4DA51" modified="2017-09-27" version="1">MIEVRLPHVTFQDLGDRIRLIWRETLYADFEKGELERAIWRKFRVSPEITVRDGALLIDTDYDKVEGYIALYIQNNLGALLRNRYTGRKVLYIHEGMDVPLLGYNAFGLIDRGTNLLQVRGVSGCNLSCVFCSVDEGPYSRTRKLDYVVDIDYLMKWFDDVAKIKGKGLEAHLDGQGEPLIYPFRVELVQALREHPNVSVISMQSNGTLLNDKLVEELAEAGLDRVNLSIHSLDPDKARMLMGRKDYDLEHVLDMAEALVNAGIDVLLAPVIIFGINDDEAEAFIEFARKIGAGKRWPALGFQNYVPYKFGRNPTIAKVVPFKEFYAWLRALEEKTGMRPLVLKPSHFGMEKREFIPLAFRPGEVVKAEVVLPGRIKGEMLAKARNRLIEVINTNAEVGDVIGVRIVRTRHGIYIATPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P851</accession>
    <name>A0A218P851_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854200.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P851"/>
    <dbReference type="GeneID" id="33315853"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06250"/>
    <dbReference type="OrthoDB" id="95543at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="193" mass="20942" checksum="B28D75ADE31998F4" modified="2017-09-27" version="1">MRRAYALALVVVVALSIAFYWGDFTFASSKTTSSVKFGSDVSTNATVNSGFCVYPDSPFGKMVAEELRNRGYKVLLLGAPVECDGQFLAVWVEWINASYTPLYAEGSIRVGAIYSSAGDPSHYLGYRNATDKEAALVNFEKTSTPQIRGYLRITVSDSSKGIIGFRGYAKHLMRSAAKSIASSVLRFPSGEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P871</accession>
    <name>A0A218P871_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06365</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P871"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06365"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="235" mass="26187" checksum="63217FAA22B11490" modified="2017-09-27" version="1">MKTVLSVFTVLVVLGAVLGMAYALAEPPSTAQVIRSIDEIHRYNFTRTVTFRQYYMNATNTNNTREVKKTFAYTGKVTATGQVDLDKGVVIEEEKFYVNGSAVMNGHVVVDLKTGEVSGSIKLLDGSEVDVLTFWREYFGLSREQALAMFRENFPLVSLRRILENSEDARMVSEGVSLSTRVLQGLGLEDKTYVYSVKTSSGRMWTLTVDSEGRPLEFVSDDETARVVVEIQPLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8B8</accession>
    <name>A0A218P8B8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_06720</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854285.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8B8"/>
    <dbReference type="GeneID" id="33315947"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06720"/>
    <dbReference type="OrthoDB" id="100909at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43483">
        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN HI_0806-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43483:SF3">
        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN HI_0806-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="226"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="244" mass="25857" checksum="2A077338F43AC1AA" modified="2017-09-27" version="1">MISYFIDFILGLGIGFIAGLLGVGGGFLIVPTLVLLGEPIHLAIGTSLACITISALASAYTHLRRGAVLFKVVLIKEAFSVPFAIIGAYLSAVTPERALRLIFAGLLLYLTYTLLRSGGKSLQESAGEVNYLRVPIVGILAGLTSGLLGISGGILNVPLFHTYVGIPMRYSVGTSSLSLLFTALAGTIAHYRLEQVDVNMVLLLAPGLMLGAHYGALTVHRVEPSLIRRLFAVLLVIIALKMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8C1</accession>
    <name>A0A218P8C1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854278.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8C1"/>
    <dbReference type="GeneID" id="33315939"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06680"/>
    <dbReference type="OrthoDB" id="50367at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07392">
        <property type="entry name" value="MPP_PAE1087"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041733">
        <property type="entry name" value="PAE1087_MPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37523:SF1">
        <property type="entry name" value="CALCINEURIN-LIKE PHOSPHOESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37523">
        <property type="entry name" value="METALLOPHOSPHOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="218" mass="23770" checksum="76252CB2009F22D9" modified="2017-09-27" version="1">MRILAITDIHGRLEKVETLTAALGGLEPELVLVAGDITNFSSANTARKVLKPLLALDIPVVAVHGNCDGRDVPELLGELGIGAHGRRVEINGLGIVGIGGSNLTPFNTIWELSEDEIKEILERNYRVGDVILSHVPPKDTKADKVHSGLHVGSTALREFIEKNQPPLVICGHIHEARSVDRIGETFIVNPGPLFKGYYAVIEFDEGEKRVKNVELRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8C2</accession>
    <name>A0A218P8C2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Endoglucanase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_06705</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="9">Binds 2 divalent metal cations per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase M42 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854283.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8C2"/>
    <dbReference type="GeneID" id="33315944"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06705"/>
    <dbReference type="OrthoDB" id="30642at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.40">
        <property type="entry name" value="Peptidase M42, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008007">
        <property type="entry name" value="Peptidase_M42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051464">
        <property type="entry name" value="Peptidase_M42_aminopept"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023367">
        <property type="entry name" value="Peptidase_M42_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32481">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32481:SF0">
        <property type="entry name" value="AMINOPEPTIDASE YPDE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05343">
        <property type="entry name" value="Peptidase_M42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001123">
        <property type="entry name" value="PepA_GA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101821">
        <property type="entry name" value="Aminopeptidase/glucanase lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR001123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ07033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="331" mass="36682" checksum="893658FDE487D972" modified="2017-09-27" version="1">MLVEELREITRIPGISGYEEKVREKLIEWIEPYADYTVDTIGNLVVELGEGELKAIFMAHMDEIGLLITGIRPDGKLTFRKIGGIDDRLLYGRHLDVITENGKLDGVIGALPVHLNLERKFDTVPWSKLVIDIGAESREEAEKLGVKVLDYAVFKKHFAVLNDRYVSTRSLDDRFGVVALVEAIKDLVDHDLDGRYIFAFTVQEEIGLKGARFLAEKYSPKYAFAVDSFACCGDLTGDVRLGGGAVIRAVDNSAIYTRALARKVAEIARRNEIPLQVGVTGGGTDASVFQHKSEVLALSVPIKYLHSEVETLHLADLEALIKLIEAIAFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8D9</accession>
    <name>A0A218P8D9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854303.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8D9"/>
    <dbReference type="GeneID" id="33315967"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06820"/>
    <dbReference type="OrthoDB" id="103563at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="149" mass="17366" checksum="D02683A78BF8B097" modified="2017-09-27" version="1">MLKELAELDSGAVLITGDGKRLARIYINAWSRGGRRVLAEYLPFQVNGDVYIGSPFESDDFEVYLIVNPLSRPKAEREKLHRWLAGHRDKLILLYEQKYVKDSIARYGIKEFIDYLIAYKRETVGFEQVDVMRLEEGKVVGSKTYIRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8E0</accession>
    <name>A0A218P8E0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA double-strand break repair Rad50 ATPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854287.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8E0"/>
    <dbReference type="GeneID" id="33315949"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06730"/>
    <dbReference type="OrthoDB" id="96601at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="195"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="316"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="405" mass="46048" checksum="D0A199435E233BD0" modified="2017-09-27" version="1">MNGMKGLALALAVLLLGSLMPLGLAKSNGTASNSTGTYTGMLDNSTREMVIAGNLVEKLQHLSKLAEDKIEPIKDKLPENSSILKNYGLAEEFKDRAIEEYNAGDYHNSILDSLTAMHYYRLALEGLKEGKEKAQDIREHIRMEVERLQEYFRFVEKTIRIAESRGIDVSNLTGLYNETKEAYGTVLEELNAGDYEKAKADLEVAQEKKALLDEELRKVREELAYKNADKIVKDFLVKGEKGIEMAEKAIQLGQEKGYNVTELQERLNAFTAVYNQVKELADEGQWEEALSVMQENRETIEEFHKAIEFIMRKVHERELQEKLTDMRAFLREMNERIQKDARALRELKGQGVDTRRAEAQLKVAVQELKVGVELLKAQKPAGARAHFAVVLEMLRRVDEFILAHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8H5</accession>
    <name>A0A218P8H5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NIF system FeS cluster assembly NifU C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_07025</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854341.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8H5"/>
    <dbReference type="GeneID" id="33316008"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07025"/>
    <dbReference type="OrthoDB" id="31224at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="12380" checksum="1B1C210A6E7564D1" modified="2017-09-27" version="1">MGDKFERLRRVIDEFNRLHGSEAESSIVSVDGNEVIIEFEGSFCATCGLYDYFDDIKWEALDFGLEIEPVEVIEAEEDEFEHGRYVVRYRLGRKSMLVPIPRKADKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8K2</accession>
    <name>A0A218P8K2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854363.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8K2"/>
    <dbReference type="GeneID" id="33316032"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07145"/>
    <dbReference type="OrthoDB" id="102023at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="120" mass="13133" checksum="CBEED31DFE19CC0A" modified="2017-09-27" version="1">MDAEEYVLVTGLLLMVLAFLLPGQLVKGTFCDGSYGKLGVYTVSVSNGYLKVSAGTGDVLLVHGDKVLLRRADIKYRYSSETGCYTLAVRQKREISLYGFVLGAVLAGGAVFYMLFLKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8L4</accession>
    <name>A0A218P8L4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Putative snRNP Sm-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_07180</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854370.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8L4"/>
    <dbReference type="GeneID" id="33316039"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07180"/>
    <dbReference type="OrthoDB" id="371816at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01731">
        <property type="entry name" value="archaeal_Sm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00257">
        <property type="entry name" value="Lsm_RuxX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016487">
        <property type="entry name" value="Lsm6/sSmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022901">
        <property type="entry name" value="snRNP_Sm-like_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001963">
        <property type="entry name" value="PRK00737.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021:SF0">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F SNRNP-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 6" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="5">
        <location>
            <begin position="4"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="76" mass="8480" checksum="3BE4EE070D277A75" modified="2017-09-27" version="1">MAERPLDVVHKSLDKDVLVLLKRGSEFRGKLIGYDIHLNVVLADADLIQEGEVVKRYGKIVIRGDNVLAISPVELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8N3</accession>
    <name>A0A218P8N3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Type 2 DNA topoisomerase 6 subunit B</fullName>
            <ecNumber evidence="7">5.6.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Type II DNA topoisomerase VI subunit B</fullName>
            <shortName evidence="7">TopoVI-B</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">top6B</name>
        <name evidence="9" type="ORF">A3L08_07335</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Relaxes both positive and negative superturns and exhibits a strong decatenase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12 13">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">Homodimer. Heterotetramer of two Top6A and two Top6B chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TOP6B family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="7"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854399.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8N3"/>
    <dbReference type="GeneID" id="33316070"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07335"/>
    <dbReference type="OrthoDB" id="65493at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00823">
        <property type="entry name" value="TopoIIB_Trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.50:FF:000028">
        <property type="entry name" value="Type 2 DNA topoisomerase 6 subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000202">
        <property type="entry name" value="Type 2 DNA topoisomerase 6 subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000062">
        <property type="entry name" value="Type 2 DNA topoisomerase 6 subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00322">
        <property type="entry name" value="Top6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005734">
        <property type="entry name" value="TopoVI_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015320">
        <property type="entry name" value="TopoVI_B_transducer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003218">
        <property type="entry name" value="PRK04184.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01052">
        <property type="entry name" value="top6b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48444">
        <property type="entry name" value="DNA TOPOISOMERASE 6 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48444:SF1">
        <property type="entry name" value="DNA TOPOISOMERASE 6 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09239">
        <property type="entry name" value="Topo-VIb_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006553">
        <property type="entry name" value="TopoVI_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="8">
        <location>
            <begin position="31"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="99"/>
            <end position="100"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="109"/>
            <end position="116"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="472"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00063696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="563" mass="63942" checksum="47CB1865A87F72E2" modified="2017-09-27" version="1">MSEANQLFKEFKIQSVSEFFRRNAAMLGYTGKVRSLTTLVHEAVTNSLDACEEAGIPPYVRVEIEELGSEHYKVVVEDNGPGIPEKYITHVFGKMLAGTKAHRNIQSRGQQGIGISGAVMFAQITSGKATRVITSTGDDEIIEAWVKIDVDRNEGKIVKKEKHPNPTGWRGTRIELEVKNVRYIRSRQGVYWYLKLTAIANPHAHIELIEPDGKLIVFPRSSEEVPKPPVEMKPHPRGVLTDDVYRMAKKTRRNTVRRFLVGEFSRISDKKVDELIEYIAALRLIKTVEDKNLQEQYYQKLMEGDVKSVLRAFRGYTKVVKQVAKLMEKPPEKLTWQEAEEIVEAFKYMKFLAPPTHGLRPIGEENIEKGLKGILKPEFVTAVTRPPKVYSGGIPFQVEVGIAYGGEIPAGFDVFRYANRVPLLFDAGSCVTTIAARSIDWKRYKVDDPDRTPLVVMINVISVHVPYTGTGKQSIANDEDIYNEIRLALMDAARRLQTYLSGKHRKLHQVKRRKTFEKYVPEIARALSVLTGEPEEAIREYFVRFIENHFASREAPEEVSENA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8P3</accession>
    <name>A0A218P8P3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Small ribosomal subunit protein uS10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rps10p</name>
        <name evidence="5" type="synonym">rps10</name>
        <name evidence="7" type="ORF">A3L08_07385</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the binding of tRNA to the ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_014011815.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8P3"/>
    <dbReference type="GeneID" id="58918905"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07385"/>
    <dbReference type="OrthoDB" id="371736at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.600:FF:000004">
        <property type="entry name" value="30S ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="entry name" value="Ribosomal protein S10 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00508">
        <property type="entry name" value="Ribosomal_uS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_uS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018268">
        <property type="entry name" value="Ribosomal_uS10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_uS10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_uS10_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005729">
        <property type="entry name" value="Ribosomal_uS10_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01046">
        <property type="entry name" value="uS10_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S10 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00971">
        <property type="entry name" value="RIBOSOMALS10"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="Ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00361">
        <property type="entry name" value="RIBOSOMAL_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS10" evidence="6">
        <location>
            <begin position="5"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="102" mass="11711" checksum="6B1DC25571CEA39E" modified="2017-09-27" version="1">MQKARIKLASTDIKALNEVTDQIKQIAERTGVRMSGPIPLPTKRIRITTRKSPDGEGTATFDRFELRVHKRLVDIEADERAMRQIMRIRVPEDVTIEIELIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Q2</accession>
    <name>A0A218P8Q2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Type 2 DNA topoisomerase 6 subunit A</fullName>
            <ecNumber evidence="12">5.6.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Type II DNA topoisomerase VI subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">top6A</name>
        <name evidence="17" type="ORF">A3L08_07340</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Relaxes both positive and negative superturns and exhibits a strong decatenase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12 13">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="11 12">Homodimer. Heterotetramer of two Top6A and two Top6B chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12 13">Belongs to the TOP6A family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="12"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854400.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Q2"/>
    <dbReference type="GeneID" id="33316071"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07340"/>
    <dbReference type="OrthoDB" id="5866at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00223">
        <property type="entry name" value="TOPRIM_TopoIIB_SPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000655">
        <property type="entry name" value="Type 2 DNA topoisomerase 6 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1360.10:FF:000011">
        <property type="entry name" value="Type 2 DNA topoisomerase 6 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00132">
        <property type="entry name" value="Top6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002815">
        <property type="entry name" value="Spo11/TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013049">
        <property type="entry name" value="Spo11/TopoVI_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036078">
        <property type="entry name" value="Spo11/TopoVI_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049333">
        <property type="entry name" value="Topo_VI_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004085">
        <property type="entry name" value="TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034136">
        <property type="entry name" value="TOPRIM_Topo6A/Spo11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003333">
        <property type="entry name" value="PRK04342.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848:SF0">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21180">
        <property type="entry name" value="TOP6A-Spo11_Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20768">
        <property type="entry name" value="Topo_VI_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04406">
        <property type="entry name" value="TP6A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01550">
        <property type="entry name" value="TOP6AFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01552">
        <property type="entry name" value="TPISMRASE6A"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56726">
        <property type="entry name" value="DNA topoisomerase IV, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52041">
        <property type="entry name" value="TOPO_IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 12 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 12 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12 13" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Spo11/DNA topoisomerase VI subunit A N-terminal" evidence="14">
        <location>
            <begin position="83"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Type II DNA topoisomerase VI subunit A all-beta" evidence="15">
        <location>
            <begin position="159"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Topoisomerase 6 subunit A/Spo11 TOPRIM" evidence="16">
        <location>
            <begin position="201"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="12 13">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00063696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF20768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF21180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ASJ07148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="386" mass="44662" checksum="E2049B0316940C0A" modified="2017-09-27" version="1">MPKRKAIHREKPKEKFSYDPKRVLSRLEEYGRQILEDIKSGKNPHFDIPMRGLSNVYFDEKRRVIRMGDKLSRRYFLNVAHARKFMQTLLIMAYVKRLVSENKHASLREAYYANKHTIPGTRENTFEDQSESDPIIEDLERMMGVLREEMHLTADRRGYIYGDIVIRDGEDEFNASRLGSGGWAVPGTVEHIQFPEINVDYALVVETAAMADRLIEEKFPKKENALIIATQGQASRGVRRLIHRLHYEEGVPIIVFTDGDPYGWYIYSTIKQGSINLAYLSDKLATPEAKFVGMTMDDIKRYGLENVTEKLKGIPPNKKGGPTGDYKRIIEEMNYPWFQNKEWQRQLKMALKMGVRIEQQALANKSLEFVAKEYLPGKINNGELLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Q6</accession>
    <name>A0A218P8Q6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation factor 1-alpha</fullName>
            <shortName evidence="10">EF-1-alpha</shortName>
            <ecNumber evidence="10">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Elongation factor Tu</fullName>
            <shortName evidence="10">EF-Tu</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 12" type="primary">tuf</name>
        <name evidence="12" type="ORF">A3L08_07380</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">GTP hydrolase that promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="10"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854408.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Q6"/>
    <dbReference type="GeneID" id="33316079"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07380"/>
    <dbReference type="OrthoDB" id="371718at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03693">
        <property type="entry name" value="EF1_alpha_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03705">
        <property type="entry name" value="EF1_alpha_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000003">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000005">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_A">
        <property type="entry name" value="EF_Tu_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004539">
        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00483">
        <property type="entry name" value="EF-1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008969">
        <property type="entry name" value="PRK12317.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10 12" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="9 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="5"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="14"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="89"/>
            <end position="93"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="144"/>
            <end position="147"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ07156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="428" mass="47584" checksum="731880D7DE959A9D" modified="2017-09-27" version="1">MAKEKPHINIVFIGHVDHGKSTTVGRLLFDSQNIPENIIQKFEQMGEKGKSFKFAWVMDRLKEERERGITIDVAHTKFETPHRYITIIDAPGHRDFVKNMITGASQADAAVLVVAATDGIMPQTKEHAFLAKTLGINHIIVSVNKMDMVNYDQKKFEQVANQVKKLLQMLGYKDVLIIPTSAWEGDNIVKKSDKMPWYNGPTLFEALDQIPEPPKPVDKPLRIPIQDVYSIKGVGTVPVGRVETGRLRVGDVVIFEPASTIFHKPIQGEVKSIEMHHEPMQEALPGDNIGFNVRGVGKNDIKRGDVAGHTTNPPTVVRPKDTFKAQIIVLNHPTAITVGYTPVLHAHTLQVAVRFEQLLAKLDPRTGNIVEENPQFIKTGDSAIVVLRPTKPMVIEPVKEIPQMGRFAIRDMGQTVAAGMVISIQKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8R8</accession>
    <name>A0A218P8R8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glutamine amidotransferase type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_07450</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8R8"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07450"/>
    <dbReference type="OrthoDB" id="350529at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026869">
        <property type="entry name" value="EgtC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42824">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42824:SF1">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YAFJ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13230">
        <property type="entry name" value="GATase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="2">
        <location>
            <begin position="2"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="266" mass="30046" checksum="CED5671F93090915" modified="2017-09-27" version="1">MCRVLFAVGRGDEMPPLVDAFVMASEKDPYKEARGKGREHRDGWGYVLFKDGNVRHYRSLRPVFEDIDAVESLKSELEGFVVLMAHSRAASQGSKNLFNTQPFGFSTRRGFSYWIYHNGDLDKGKIIEMAEFEEKDLESASDSYAMAAYLCRRLESFEASELLKHYSALAKTAKTSFNTGTLFLGSDGTAAGFVTAYSKPLYIMNPKNWDYVRQNVLREDGLFAVGSSTLELYFKAEWKPVVNGTAFYVDIDFENEAFNVKELVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8V5</accession>
    <name>A0A218P8V5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_07700</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854459.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8V5"/>
    <dbReference type="GeneID" id="33316143"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07700"/>
    <dbReference type="OrthoDB" id="387158at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="74"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="257" mass="30349" checksum="3C0CF35DF10BF8A9" modified="2017-09-27" version="1">MRELKWKIQNTREGAKCMNSFKWQIIVLFAIFILWTGAGYLFRDTLLVESALVVATIFMAGFVAYQAWATIVMAQQAQEQVKQMIRQAQLTQRSMEEMQKQVAIMGETLMEMKKDRMRIEELKDSLVSYIQYLIKALNASSPNYVLVEKPYPYFYKRYRTRIGTSLSPSEEGWVWRISKFIERLLEKKGFSLDELEEYNGMIAKFKKPGTPREEKHRLAAELEKKSKEMRQIAEDIFHEIKEMGEYAFEREIMANRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8V7</accession>
    <name>A0A218P8V7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8V7"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07680"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="22"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="150" mass="17389" checksum="695DB7C52AC1F7D7" modified="2017-09-27" version="1">MGIGNFLKKVGDATKKAMDRAAKEAKYRAKALDIKREIAEAERKFREEAARKEFEAKREILSQLKMRQLEAVCAAKGIPTYRTQIVNGEERRYKIRNKDELIDVVASHLTLEEVAEVAKRYKVKSRHIIQHFQKWLEEANEALGAFKEQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8W9</accession>
    <name>A0A218P8W9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854485.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8W9"/>
    <dbReference type="GeneID" id="33316174"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07845"/>
    <dbReference type="OrthoDB" id="31168at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051782">
        <property type="term" value="P:negative regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050625">
        <property type="entry name" value="ParA/MinD_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384:SF10">
        <property type="entry name" value="ATPASE INVOLVED IN CHROMOSOME PARTITIONING, PARA_MIND FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384">
        <property type="entry name" value="SEPTUM SITE-DETERMINING PROTEIN MIND HOMOLOG, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="1">
        <location>
            <begin position="11"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="240" mass="27451" checksum="E820C13A937FB7BE" modified="2017-09-27" version="1">MPVIGVTGPEGAGKTTLAINIGGYLAVNGARTLLIDANLYFPDMSFYLDMRPNYPLHLYLEDHEMDIEWLVTPHEGLKNLYLILGEPMKPIQRRYSFKLMTFLIAYLREHYGVIIVDFPPGLPVEPLPIIPEIDHQILVIDPTIVPLRNLKMWVESLIIKFSHMGAEKLWVLINKPLIPEKNLIELEDFIVDELGIPVIGTIPYDVEIHESTRTGTLLCEMWEMENPVSELAYSIEELFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8X7</accession>
    <name>A0A218P8X7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_07790</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854474.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8X7"/>
    <dbReference type="GeneID" id="33316160"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07790"/>
    <dbReference type="OrthoDB" id="6762at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054609">
        <property type="entry name" value="PF0864-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF7">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22482">
        <property type="entry name" value="AsnC_trans_reg_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="4"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="160" mass="18212" checksum="8648D1A5B6E28717" modified="2017-09-27" version="1">MVQLDDLDRAILRVLKEDARLTISEIAERLNRPESTIHFRIKKLQERNVIDKYTIVLGEDLQPRALAVVYIQAETPIIEDFLERYVKHIIKTLSLLPNVLAVARSGKGGVIALIGEESSEKLNRFVDETIRVLPTLKRVEVMHIDEFGKGKDLVGFLVNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Y1</accession>
    <name>A0A218P8Y1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TldE-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_07870</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854490.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Y1"/>
    <dbReference type="GeneID" id="33316179"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07870"/>
    <dbReference type="OrthoDB" id="84520at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="entry name" value="PmbA/TldD superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43666:SF1">
        <property type="entry name" value="CONSERVED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43666">
        <property type="entry name" value="TLDD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="Putative modulator of DNA gyrase, PmbA/TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloprotease TldD/E central" evidence="2">
        <location>
            <begin position="115"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloprotease TldD/E C-terminal" evidence="1">
        <location>
            <begin position="218"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="440" mass="49512" checksum="3D0F11FBD6438D20" modified="2017-09-27" version="1">MFDVNELILKKAKELGFGDVVVLSHEMNRRQVRFANNEITVAKNWHERKVELFVELEKRIAGTTITELSEENIERTLKTLLSNMKGMAPKEDYYGIAEGPFEYRDIPETFDKAIVELDEPNEYVERAINAALEEGAKRVAGVLYTDHDRIYLTTSNGVEAFDEGTGIEISVRAFIGDLESGHGTNSVRVLKKFDPESAGRKAGEIASLARNPEQGPEGKFDVIFDPLAFANLLSYMSFMTSAYAAEAGFSFLVNKLGQKVANEIVTIKDVGNMPNGYGTRKFDDEGVPTRETAIIENGTFRTFLLNTSLARKYKTETTANAGLIMPHAWNIVLEPGDYSREELFSEVKRGIYITNVWYTRFQNYVTGDFSTIPRDGIFLVENGELRPIRNIRVSDNLQNILESIAALTKESHHVHWWEVDTPVSTPYVLVKDVGITRATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Y7</accession>
    <name>A0A218P8Y7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF835 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_07875</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854491.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Y7"/>
    <dbReference type="GeneID" id="33316180"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07875"/>
    <dbReference type="OrthoDB" id="101917at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008553">
        <property type="entry name" value="DUF835"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33531:SF7">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33531">
        <property type="entry name" value="RUBRERYTHRIN SUBFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05763">
        <property type="entry name" value="DUF835"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="DUF835" evidence="2">
        <location>
            <begin position="227"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="358" mass="39954" checksum="4B4BB6FC86D95BA3" modified="2017-09-27" version="1">MLEILNVIMRFLTWGFATYRWRKRGEYFMLLLSIALWMDFLAALTQKQVLSDFGLNPNLASLIPLMSLMAVFEGILLIAASLSLRDRIKTLWGQFLLLACGIAGPTYVLLTVLLDASPLVITAFPVPFMGVSLMFLGYSLIKEEVGLKTVSTLFPVGAFLLGAINLTYPLTARTSVASYFYGLGALFRAMMFLGMMRCAFLTVKPPEMPITELPTGAFYSDSGRAFDILLQEMQSSGNGVLITRKSLEGFKPKFPVFWMTKVASGMIGENIIALSPTNIGILIDLVKRHLEKGHSLVVIDCFEYLMVENGFENAFKFLLSLKDTVVKYNGTLVVVIEPSAYSKKQMAMLMREFERLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P942</accession>
    <name>A0A218P942_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_08150</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 57 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854539.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P942"/>
    <dbReference type="GeneID" id="33316236"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08150"/>
    <dbReference type="OrthoDB" id="18576at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003844">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030979">
        <property type="term" value="P:alpha-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040042">
        <property type="entry name" value="Branching_enz_MT3115-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004300">
        <property type="entry name" value="Glyco_hydro_57_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41695">
        <property type="entry name" value="1,4-ALPHA-GLUCAN BRANCHING ENZYME RV3031-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41695:SF1">
        <property type="entry name" value="1,4-ALPHA-GLUCAN BRANCHING ENZYME TK1436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03065">
        <property type="entry name" value="Glyco_hydro_57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 57 N-terminal" evidence="3">
        <location>
            <begin position="17"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="361" mass="40503" checksum="7AE21F4BC2CE4886" modified="2017-09-27" version="1">MRALVFHGNLQYAEIPKAEIPKVIEKAYLPVLNELLKAEVPFGLNVTGYTLGLLSEEVLSLIRDGIETGLIEIIGTSYTHAILPLLPLDRVEAQVVRDREIKEELFGVSPRGFWLPELAYDPIIPAILRDNGYEYLFADGEAMLLSNHLNSVVKPIKPLYPHLIKAQRGEGNVYLNYLLGLRELKKAIGLAFGGKVTLKAVKDIEAVPVWVAINTAVMLGIGRFPLMSPKKVAGWLREKDDVLLYGTDIEFIGYRDLAGRRMDVKGLLELLEELGTEPCLPSRMRHSGKELYLRTSSWAPDKSLRIWTEDGGNARLNMLTPSAKGELAFLAENSDARGWEPLPERRLDAFRAIYKSWRGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P947</accession>
    <name>A0A218P947_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MIP18 family-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854561.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P947"/>
    <dbReference type="GeneID" id="33316259"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08265"/>
    <dbReference type="OrthoDB" id="371709at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIP18 family-like" evidence="1">
        <location>
            <begin position="28"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="126" mass="14227" checksum="2CDEC415CD38018F" modified="2017-09-27" version="1">MGLLGIFKKPAKRPKKGPREDLPPEVRRVVEVLRSVKDPETELNIVDEGLLYGLTVEGDKVQAFLLLARSTPECHFCQSIAVNVQRRILRDIVDVLRKEGFKRIEVYNEIGLLLEEWGDKDGSAGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P981</accession>
    <name>A0A218P981_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Carbon starvation protein CstA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_08430</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854593.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P981"/>
    <dbReference type="GeneID" id="33316292"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08430"/>
    <dbReference type="OrthoDB" id="77715at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051605">
        <property type="entry name" value="CstA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003706">
        <property type="entry name" value="CstA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30252">
        <property type="entry name" value="INNER MEMBRANE PEPTIDE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30252:SF0">
        <property type="entry name" value="PEPTIDE TRANSPORTER CSTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02554">
        <property type="entry name" value="CstA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="460"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="488"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="518"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="546"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="CstA N-terminal" evidence="7">
        <location>
            <begin position="2"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="CstA N-terminal" evidence="7">
        <location>
            <begin position="385"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="584" mass="63652" checksum="5CDECF98AE9045A6" modified="2017-09-27" version="1">MNSSVVILLAAVIYLGMYFTYGKGLQNKVVKADPNRPTPAHRLYDGVDYVPAHPLVLYGHNFASIAGAGPITGPAIAMAWGWLPGLIWVWFGNVFIGAVHDYLALMSSVRYDGKSVQWIAGKLMSRRTGVAFELYVWFTLLLVVAAFVAVTAKLLTTTPEAATATLLFLVVAVILGWLLYKMKMEFKLATLVGLILLAIAVWLGLKYPLVFVNGQTDATSAAYTTAYHYWNIILMVYIIIAASLPVWVLLQPRDYLNAYILWFGLLFGGIALTLLAKNFTAPAYTTWSAHVVTGVTSEGSKAVASPFWPTIPLIIACGSLSGFHSLVGSGTTSKQLDNEIHGLLVGYGGMFTEGFLATVVITSIAVYGVQLTGLQPDQWGLHYIEKGGLGAFIGSYAKAVSEFYGVSETFGKTFATLWVSAFTLTSLDTATRLGRFAWQELFGMVADTSKGAVKFITNKWVASIIIAGLGTYLAWGASYKVIWPAFSAMNQMLASIALMTGALWVAKVQRAGSWSWAVLIPAIFLWITVTAAMIWYIIYVPMVGRYLIAVKGALVVSLLLNFLLAWDFWVAWKRPEEEYAASAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P986</accession>
    <name>A0A218P986_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08365</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854580.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P986"/>
    <dbReference type="GeneID" id="33316279"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08365"/>
    <dbReference type="OrthoDB" id="101536at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12730">
        <property type="entry name" value="ABC2_membrane_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="217"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="247" mass="27572" checksum="F38F1490F4CEB12C" modified="2017-09-27" version="1">MKEVLRWELRSPLNLAMTSGGAVLIGLVMKRYYMTWSASSHGGPYGVDILGSIFRDAFTGDVYLLLALLVPFLVTLAVRLERDEGVALSVYSLPVSRVRILLAKFLAAFLVLFIFVFSVHLLLFYLHFADTPKAVIGVLKVQAIPMAFFYLSALLFMVSVAAVVAIASPNTYVSIFGGFVLLYLPEFLGAGWFWLLKWRDALGNYKWATISLSMDPVFGWEFIKASLLPALVLTALYLLVGNWRDVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P994</accession>
    <name>A0A218P994_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dinitrogenase iron-molybdenum cofactor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08485</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854603.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P994"/>
    <dbReference type="GeneID" id="33316303"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08485"/>
    <dbReference type="OrthoDB" id="84967at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.130">
        <property type="entry name" value="Dinitrogenase iron-molybdenum cofactor biosynthesis domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003731">
        <property type="entry name" value="Di-Nase_FeMo-co_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036105">
        <property type="entry name" value="DiNase_FeMo-co_biosyn_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051840">
        <property type="entry name" value="NifX/NifY_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33937:SF5">
        <property type="entry name" value="IRON-MOLYBDENUM COFACTOR-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33937">
        <property type="entry name" value="IRON-MOLYBDENUM PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02579">
        <property type="entry name" value="Nitro_FeMo-Co"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53146">
        <property type="entry name" value="Nitrogenase accessory factor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dinitrogenase iron-molybdenum cofactor biosynthesis" evidence="1">
        <location>
            <begin position="13"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="138" mass="16171" checksum="43CDE65112E22C3F" modified="2017-09-27" version="1">MRCLKVAFGMEDDEHLIDAHYGDSEFFAIYEVCEDGSVKLLEKRPNKAKDFEEEHDEGHGNPRKFKAVVSQLLDVDVLAAFRMGPNFLRIRDKTNKVAFFTRTRDLKLALQRIVENFDELWEQVQEKKRAIEKPIVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9B8</accession>
    <name>A0A218P9B8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Class II glutamine amidotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_08670</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854631.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9B8"/>
    <dbReference type="GeneID" id="33316340"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08670"/>
    <dbReference type="OrthoDB" id="350529at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01908">
        <property type="entry name" value="YafJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026869">
        <property type="entry name" value="EgtC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42824">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42824:SF1">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YAFJ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13230">
        <property type="entry name" value="GATase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="2">
        <location>
            <begin position="2"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="322" mass="36902" checksum="6BA57208F50AA7C0" modified="2017-09-27" version="1">MCELFSVNANKRVGISFTWKGFVRKGEYNPDGWGIGWYVTATNGKRAASLIKQPIPACRSRIAPSLPRLNVKSHILVSHVRYATSEINYVNTHPFVRRVYSVGQYDEWIFAHNGVLNGVEELPTRLQPLGTTDSEAAFCYIMENLEGTPTIRELFLKLLDLLDELSDYGTLNVLMSNGRYLFAYSHYPGKGMWLLKRHPPHRGRARLLDEDFEVNVGEMKAPDEYAYLAATRKLTDEKWKRMRPKTLHIFRDGALLLTIRDRIEPMLDEDSIEVLMAILNGESVEIDETVRRLVDIKLLKTTGNGVEINDHRRAIVRLIVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9C7</accession>
    <name>A0A218P9C7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative methylthioribose-1-phosphate isomerase</fullName>
            <shortName evidence="2">M1Pi</shortName>
            <shortName evidence="2">MTR-1-P isomerase</shortName>
            <ecNumber evidence="2">5.3.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">MTNA-like protein</fullName>
            <shortName evidence="2">aMTNA</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">S-methyl-5-thioribose-1-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_08590</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>5-(methylsulfanyl)-alpha-D-ribose 1-phosphate = 5-(methylsulfanyl)-D-ribulose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:19989"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="ChEBI" id="CHEBI:58548"/>
            <dbReference type="EC" id="5.3.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EIF-2B alpha/beta/delta subunits family. MtnA subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.23" evidence="2"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854619.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9C7"/>
    <dbReference type="GeneID" id="33316323"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08590"/>
    <dbReference type="OrthoDB" id="45195at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046523">
        <property type="term" value="F:S-methyl-5-thioribose-1-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.420:FF:000001">
        <property type="entry name" value="Methylthioribose-1-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10470:FF:000006">
        <property type="entry name" value="Methylthioribose-1-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.420">
        <property type="entry name" value="translation initiation factor eif-2b, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01678">
        <property type="entry name" value="Salvage_MtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005251">
        <property type="entry name" value="IF-M1Pi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011559">
        <property type="entry name" value="Initiation_fac_2B_a/b/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027363">
        <property type="entry name" value="M1Pi_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00524">
        <property type="entry name" value="eIF-2B_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004326">
        <property type="entry name" value="PRK05720.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00512">
        <property type="entry name" value="salvage_mtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475:SF1">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1 2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="57"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="257"/>
            <end position="258"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="2">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="357" mass="39786" checksum="3B43CC55A1CC93D1" modified="2017-09-27" version="1">MELRYEPEELTRLPRSVRYEKGKVIMIDQTLLPREFRTIELTTVDQVAEAIVTMKVRGAPAIGASAAFGLALYADTTKAKTKEEFMDGFYSAYERLKNTRPTAVNLFWALNRIKKLVEENLESPLDEIKRLIVNEAQKIADEDVEANLRMGHYGAEVLPEGNVLTHCNAGSLATVQLGTVGAVLRVMHRDGTLKLLWVDETRPVLQGARLSAWEYHYDGIPLKLITDNMAGFVMQQGKVDAIIVGADRIVANGDFANKIGTYSLAVLAKEHGIPFFTVAPLSTIDMSLKSGKEIPIEERKPEEVLTCGGCKIAPDVDVYNPAFDVTPHKYLTGIITDQGVVWPPFERNLKRLFKEQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9F7</accession>
    <name>A0A218P9F7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854671.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9F7"/>
    <dbReference type="GeneID" id="33316383"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08885"/>
    <dbReference type="OrthoDB" id="96856at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="301" mass="33936" checksum="0CEC6046E27209E6" modified="2017-09-27" version="1">MKLSRIKLLTGLLVALILVLSASTIQWIAYENARTKASALPCNLSPAPYRLEVESFLPPEELLDRAVGHRYNDGELEILVPEKIGSMDPSYKHLTWLLSWKLNDSVSSLRDFLDNGSDEEVQMAFQVVKRYYLMLRLEKEYCRKNLTAVVFRPSPPLKWLLSMKSSAGKLAGSPDFSASLLALSVAFLISALFWLWLFLVSKLSLRGHLRVMVAFVLLILFFGSMIFALNSLLSSGMDDGKSPSSQECDVWHTMDCYSLLFNEAFDHMDSAATCETLDYLRDRLDAQEMGRLEEALGVAYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9K6</accession>
    <name>A0A218P9K6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Molybdate/tungstate import ATP-binding protein WtpC</fullName>
            <ecNumber evidence="11">7.3.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">A3L08_08985</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Part of the ABC transporter complex WtpABC involved in molybdate/tungstate import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>tungstate(in) + ATP + H2O = tungstate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35027"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46502"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">The complex is composed of two ATP-binding proteins (WtpC), two transmembrane proteins (WtpB) and a solute-binding protein (WtpA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ABC transporter superfamily. Sulfate/tungstate importer (TC 3.A.1.6) family.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.6" evidence="11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854688.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9K6"/>
    <dbReference type="GeneID" id="33316403"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08985"/>
    <dbReference type="OrthoDB" id="18368at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015417">
        <property type="term" value="F:ABC-type polyamine transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901238">
        <property type="term" value="F:ABC-type tungstate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000425">
        <property type="entry name" value="Probable ABC transporter, ATP-binding subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050093">
        <property type="entry name" value="ABC_SmlMolc_Importer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005893">
        <property type="entry name" value="PotA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013611">
        <property type="entry name" value="Transp-assoc_OB_typ2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01187">
        <property type="entry name" value="potA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42781">
        <property type="entry name" value="SPERMIDINE/PUTRESCINE IMPORT ATP-BINDING PROTEIN POTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42781:SF4">
        <property type="entry name" value="SPERMIDINE_PUTRESCINE IMPORT ATP-BINDING PROTEIN POTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08402">
        <property type="entry name" value="TOBE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="MOP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="4"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00057369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ASJ07443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="354" mass="39721" checksum="D354FDF236BFF3C8" modified="2017-09-27" version="1">MVDVKLENIVKTFGETVALKGINLHIKAGELFTLLGPSGCGKSTTLRIIAGLDFPDSGTIYFGDEEVTYLPSSKRGAVLVFQNYALWPHMTVFDNVAYGLKLRKVPKAEIEKRVKWALELVKLQGFEDRYPTQLSGGQQQRVAIARALVVEPKVLLLDEPLSNLDAKLRLEMRSEIRRIQRELGITVIYVTHDQEEAMAISDRIAVMNVGTVEQVGTPKEIYETPKTEFVASFMGKTNVIPAKVVERNGDRVTVEFEGIRLDGLHYTEKSDDVVVVIRPERIKLKPVENAVSFTGTVDLVEYYGFFIEVVGLFGDTRIIARTISDRDVAGLRPTQPVTFYVERDDIIVLPKQHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9M1</accession>
    <name>A0A218P9M1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Peptide transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09210</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854728.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9M1"/>
    <dbReference type="GeneID" id="33316448"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09210"/>
    <dbReference type="OrthoDB" id="85097at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd08769">
        <property type="entry name" value="DAP_dppA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.130">
        <property type="entry name" value="Dipeptide transport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10780">
        <property type="entry name" value="Dipeptide transport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027476">
        <property type="entry name" value="DppA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007035">
        <property type="entry name" value="Peptidase_M55"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036177">
        <property type="entry name" value="Peptidase_M55_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04951">
        <property type="entry name" value="Peptidase_M55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015853">
        <property type="entry name" value="Pep_DppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63992">
        <property type="entry name" value="Dipeptide transport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="280" mass="30640" checksum="57567C40DD3FC547" modified="2017-09-27" version="1">MRAFISLDLEGLPYIVSREHLFVKGALYAEARRIATEIVKTLARALHENGFDEVVVADSHGPMVNVLPEEMPEYVELVRGFPRPLSMVAFAKGSDAALFLGYHAKAGTGYATFDHTYSGASVDRLEINGVEVSEFLLNSYLLGSWGIPVILVGGDRRLIETDVRNHAPWVVGIPFKEASSRYAAKSPSMARVKGMLEAGVREAVERLKTGKAKPLVTEEPVHVILRFLNSAFADAAELLPAVKRVDGKTVEFEAEKVEDAYRIFELLTLAAAGVNAVVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9M9</accession>
    <name>A0A218P9M9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854722.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9M9"/>
    <dbReference type="GeneID" id="33316442"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09180"/>
    <dbReference type="OrthoDB" id="86183at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="82" mass="9034" checksum="153E76A313D06EC2" modified="2017-09-27" version="1">MKVRLGYPDRIVEVDGEAVYVFRGRLVSAPLKEVVRYYRGEDALLPPAIREISRDVVAAILRTSALQNEPSAALQALHSISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9N2</accession>
    <name>A0A218P9N2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854738.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9N2"/>
    <dbReference type="GeneID" id="33316462"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09280"/>
    <dbReference type="OrthoDB" id="102066at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="108" mass="11955" checksum="581253FD0ECECDB6" modified="2017-09-27" version="1">MIEFVILLGVIGGWIVVASTLFLMLALGQTWGLVGVLLLVGFILVNQSLKAKYMRTIVDATPRAKAIAAHIFEMNELILLSSYLVSLLLYEGIQKYVEIIIKFPDLMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Q2</accession>
    <name>A0A218P9Q2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854753.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Q2"/>
    <dbReference type="GeneID" id="33316477"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09355"/>
    <dbReference type="OrthoDB" id="101324at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="77" mass="8311" checksum="65C27ED3B02E334A" modified="2017-09-27" version="1">MEESYDTLETLGAVVSALGLAVILITEGRALGLGLLPITIGTVVWKMGEMRRELKGELARLREELELLKSISGVENG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9S1</accession>
    <name>A0A218P9S1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Topoisomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_09255</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854733.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9S1"/>
    <dbReference type="GeneID" id="33316457"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09255"/>
    <dbReference type="OrthoDB" id="86047at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01027">
        <property type="entry name" value="TOPRIM_RNase_M5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.400">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034141">
        <property type="entry name" value="TOPRIM_RNase_M5-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Toprim" evidence="3">
        <location>
            <begin position="5"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="255"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="141"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="255"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="292"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="303" mass="33875" checksum="98C9A33A1F5FE2DD" modified="2017-09-27" version="1">MTIVDVRILVEGASDVEVVSKALQGLALGSEYNITISAIIPTTNVEIAKSAAAGADLLIIATDADRVGRDLAERLYNELNEMVGHVERMKLPLGHDLEHVDVELVRKELRNTLVRAGLKSLQVLPGYMEIRKELLDVKGKYDQLANDYEALYKEHEELQKKYEELHAEYTRLRSENEGLRELLTKKSRPVKIEDAWKGLFPAEPVPEERILALAVEKLGLAGKIIVGQGYIFAEDESLIEELLKTVYLSLAVKEELEEKKEPKPPKPPEGKPSASKPEETNIVENPEVNPDDIERLLKGVGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA05</accession>
    <name>A0A218PA05_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cation:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_01760</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA05"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01760"/>
    <dbReference type="OrthoDB" id="19265at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050622">
        <property type="entry name" value="CPA3_antiporter_subunitB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007182">
        <property type="entry name" value="MnhB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006248">
        <property type="entry name" value="PRK08386.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33932">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33932:SF4">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04039">
        <property type="entry name" value="MnhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Na+/H+ antiporter MnhB subunit-related protein" evidence="7">
        <location>
            <begin position="4"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="144" mass="15100" checksum="3D622F4A2C4BC533" modified="2017-09-27" version="1">MGVIVRTGARATIPLIGIFGAYIVAHGHLTPGGGFQGGATIAGAGILFLLAFGLSEMKRHFNKNLYSALEGIGGLAFLGAAMLGIGTAFFYNVLWHNGPFFNGKPGTLLSAGYLPIMNLAVGLKVFTGLVSAMVGLALYRRWRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA82</accession>
    <name>A0A218PA82_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA82"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08600"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="129" mass="14731" checksum="F9D95FF40FC524B2" modified="2017-09-27" version="1">MKISRSFSRFQLGDEEYEENYGKVIKSLLSTGEHKFFVATDPYGRYLGHVWVCLKIDTADFVPSAYIYGIETLFPGMGIGSALLKKAEEWAKRVGARKISLRVDVNNPARKWYAEKGYSERAVIMEKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PAA6</accession>
    <name>A0A218PAA6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Polymerase/histidinol phosphatase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854939.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PAA6"/>
    <dbReference type="GeneID" id="33316412"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09030"/>
    <dbReference type="OrthoDB" id="9968at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042578">
        <property type="term" value="F:phosphoric ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050243">
        <property type="entry name" value="PHP_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006251">
        <property type="entry name" value="PRK08392.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36928">
        <property type="entry name" value="PHOSPHATASE YCDX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36928:SF1">
        <property type="entry name" value="PHOSPHATASE YCDX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase/histidinol phosphatase N-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="217" mass="24345" checksum="6BF97673D5F21A6B" modified="2017-09-27" version="1">MMFPHDTHTHTVYSDGTGAIADNIAAAEERGLKVLAITDHSRYLGPKTVNRYVREVRRWAEDSEVMVLVGIEGNITGNGVDVPAFMAEKLDFVIASVHEWLETPEQYLELVKIALMDEDVDVIGHFGANFPYIGFPRAEELEEILELAEANGKAFEISSRYKVPDVEFIRECVRRGIKLTFASDAHTPRSVGNVGWSERAFIKAGGKKEDLLFEEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P4Y7</accession>
    <name>A0A218P4Y7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_00070</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853102.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P4Y7"/>
    <dbReference type="GeneID" id="33314618"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00070"/>
    <dbReference type="OrthoDB" id="9623at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.680">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051081">
        <property type="entry name" value="HTH_MetalResp_TranReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33154">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ARSR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33154:SF33">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR SDPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ05839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="198" mass="23348" checksum="05C667A07FA721D4" modified="2017-09-27" version="1">MESNKNLGRLLDILGNETRRRILILLTKRPYFVSELSQELGVGQKAILEHLRILESAGLIEGRTEKIPRGRPRKYYTIKRGFRLEVLLTPYAFGTEMYEPKAPRQTKEYDQARSLIKSTEPAEAKIDELLAFLEEIQNRISEIIQTKQELEEVRLLTETYIENLFRRIAQENEREFERLLKEFAPRLPRKILEDLDGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P508</accession>
    <name>A0A218P508_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aminotransferase</fullName>
            <ecNumber evidence="7">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_00190</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853126.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P508"/>
    <dbReference type="GeneID" id="33314642"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00190"/>
    <dbReference type="OrthoDB" id="372018at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000033">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050596">
        <property type="entry name" value="AspAT/PAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006229">
        <property type="entry name" value="PRK08361.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383:SF3">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="8">
        <location>
            <begin position="34"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ05863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="392" mass="44832" checksum="47ABEE36A6AFB8B4" modified="2017-09-27" version="1">MKYKKRKYFLAGRINLIQRSKIRELFEKAKKMDDVISLGIGEPDFDTPEVIKEAAKRAIDEGYTHYTPNAGIPEFREAIAEYYKTHYKVDVSPNDIIVTAGAYEATYLAFQTLLEQDDDVIIPDPAFVCYVEDAKIAEAGIIRIPLREENEFQIDPDELVEAITKRTRMLVINYPNNPTGAVLKKKTVKAIADIAEDYNLYILSDEPYEHFLYEGAKHYPMIKYAPDNTILANSFSKTFAMTGWRLGFTIAPTQVIKDMIKLHAYVIGNVTSFIQIAGITALRDKRSWDALETMRQTYAERRKLVLHHLHKMPHITPFKPKGAFYIWAKIDPELDMTSEDFANWLLENAGVVVIPGTAFGKQGEGWVRISYATEKEKLIEAMERMNEALSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P519</accession>
    <name>A0A218P519_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">AsnC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00305</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853147.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P519"/>
    <dbReference type="GeneID" id="33314665"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00305"/>
    <dbReference type="OrthoDB" id="8865at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="1">
        <location>
            <begin position="6"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="81" mass="8898" checksum="ABD892A014987866" modified="2017-09-27" version="1">MVRSYVLLTVEIGKVESVIEALKQIPGVTKADAVTGPYDAIVHIEANDLGELTRRILHDIHNIDGVIDTTTAIVVEMEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P534</accession>
    <name>A0A218P534_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853133.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P534"/>
    <dbReference type="GeneID" id="33314650"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00230"/>
    <dbReference type="OrthoDB" id="99520at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="62" mass="6836" checksum="061C30F27FB78527" modified="2017-09-27" version="1">MNEILIISALFLVCGLLLGIFIAKTILKDIGIPPDERALELDKLTALRTLELIMQGRCEVSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P545</accession>
    <name>A0A218P545_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Peptide ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_00290</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853144.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P545"/>
    <dbReference type="GeneID" id="33314662"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00290"/>
    <dbReference type="OrthoDB" id="312811at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050366">
        <property type="entry name" value="BP-dependent_transpt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025966">
        <property type="entry name" value="OppC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43386:SF1">
        <property type="entry name" value="D,D-DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DDPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43386">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN APPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12911">
        <property type="entry name" value="OppC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="144"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="341"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="142"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ05881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="392" mass="44045" checksum="76C8F458DD54FE4F" modified="2017-09-27" version="1">MGREEYKTNVLDRLSDALVEALGSFFSLFRKDWKKKNRSKMEEWKLMLYALNRSPPGLIGLLLVLMFVFLGLFGPSLATWSYRFFPTNYNFDTYLAPPGSTYTLNVTEIQGDNVIQYITTIHYPLGADHYGRDLLSLILYGARVSFVISIIVIALGVPLGIILGLVAGYYGGKVDELVMRITDVFLAFPALILAIAFSAVLPQRLQNFISAHESIQRFVLWLFALEQQDAGNLGKLLAVILAMIIVWWPAYARITRGSTLTERESLYVEAARAIGLSSRTIMFRHILPNIIGPILVYITLDFGGVILMEAGLSFLGLGATPPIADWGRIVYDGSQYFPEKWWLVTFSGFMIMLVALGWNLLGDTMRDILDPKTRRSIEFKVKKAKKEGESNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P546</accession>
    <name>A0A218P546_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cation:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_00370</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853160.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P546"/>
    <dbReference type="GeneID" id="33314678"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00370"/>
    <dbReference type="OrthoDB" id="84883at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007208">
        <property type="entry name" value="MrpF/PhaF-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006243">
        <property type="entry name" value="PRK08381.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34702">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER SUBUNIT F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34702:SF1">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04066">
        <property type="entry name" value="MrpF_PhaF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ05897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="91" mass="10075" checksum="B3DA210065FC60FA" modified="2017-09-27" version="1">MAQESLLVSAFYLLVFTAVLITYRVLRGPTLPDRVVGLNTVTTKVVVIIALVSVMREEYYLIDLAIVLLMVNSVGGLILAKYMERRGRSGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P566</accession>
    <name>A0A218P566_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF505 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_00495</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853182.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P566"/>
    <dbReference type="GeneID" id="33314703"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00495"/>
    <dbReference type="OrthoDB" id="63976at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007548">
        <property type="entry name" value="DUF505"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04458">
        <property type="entry name" value="DUF505"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ05919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="569" mass="64665" checksum="FD14C1F759FD4030" modified="2017-09-27" version="1">MYLKKRHIEILREMKKTESQAEIETKLPEEFQIRAIELYILGFAELEGGKIKLTEAGRKLLEISDSLNLAELPDVIADTEIMKMLELLEETGKISESWLEKLKERKLADENGLTEFGKALLQIYRETHPVVYLTPEIASFLRGMPKLGTLDELVTFKNSRLYGDNIVNALQAMRLLLISPPTEKGRAFTTTPAAKLALRALNMIPVFARAIVLRKEDFEALKAGKTNAELESMGLSDERGATEFGKAMMETYEAMGRVEEKVLPIYLLEDELNVLKAIQEIEKKYETNPDILPTEKEIAKRVDVEDLGAVLHLLESKELIERRLVKNKDTYWLTEWGKEAITFGTVSPDAMKAVTYAESGDVPIAEWVIKAQEEGVVKAGITDKGRFYLKLSREIRRKPYLTRYDAAILAKLPKKKYIHRNELIELVQNYVGGDEKEIVRAIGEAEAKGFVVELQNEMVKLTELGEKVKTALENAKLQEIVKVKFSVTPTLYNVLKVIYDNLETFNRIWKEKGEARGYKMEEVDVIRKHLSLSDDEIKKALTMLRELGFLGSKSLTEAGKTLVEAYMAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P573</accession>
    <name>A0A218P573_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Radical SAM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_00565</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853195.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P573"/>
    <dbReference type="GeneID" id="33314717"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00565"/>
    <dbReference type="OrthoDB" id="15538at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040086">
        <property type="entry name" value="MJ0683-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43432">
        <property type="entry name" value="SLR0285 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43432:SF3">
        <property type="entry name" value="SLR0285 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01084">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="4">
        <location>
            <begin position="14"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ05932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="280" mass="32681" checksum="13E5920771BE2A92" modified="2017-09-27" version="1">MYVRPFDPWKAKLCTCPFKYTLNVYTGCDHACVYCYITSYIPNAFRVRTKENLLSKLERELRKFDKRHIIALSYSSDPYPTIERELGVTRKVLELFKRYGVRCLLLTKSDIFERDIDILSELKCAVGITVTTVDERKARMLEPNAPSPRARIRALKKAKEAGIPVYARIDPIIPFYTWEDFDETLDALSFVSHITVSTLKLRADSKKRMFAKFPELRGKLWPLYEKGERIGGYHYLPQELRFEILREAERKILKRGITFGSCREGYRSFPSCDGSHLVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P579</accession>
    <name>A0A218P579_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_00620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853205.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P579"/>
    <dbReference type="GeneID" id="33314728"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00620"/>
    <dbReference type="OrthoDB" id="85425at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ05943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="161" mass="19430" checksum="7F0B066B9DB99F2C" modified="2017-09-27" version="1">MIKVKVMRPIFHPYTEERDFVLYPYHIFHLRLFYRRLGMKDKVMDYFAYVDLYRLGAERGDDFIPLEEWNVDERKLMEPLVDYEEAEKKAFESAITWGNSRVVSWWLPRIEVVREEMAYKVFWIYERNGEKLIMDSLDGKEYSLSAMVGKNGRSCKGPFCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P587</accession>
    <name>A0A218P587_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sarcosine oxidase subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_00670</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853214.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P587"/>
    <dbReference type="GeneID" id="33314738"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00670"/>
    <dbReference type="OrthoDB" id="168391at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF287">
        <property type="entry name" value="FAD-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="2">
        <location>
            <begin position="3"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ05952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="388" mass="43292" checksum="7C8AD02ACEAEDC8C" modified="2017-09-27" version="1">MSKVAIIGGGIIGVATAYELAKLGEEVILFEKNYFGSGSTFRCATGIRAQFTDEANIRLMKYAVERWEKLEEELGFDINFRQTGYLFLATSEEEIEAFKNNIRLQNKFGVPTRLIDMDEAKEIVPILNTEPFLAGAWNPKDGKANPFKTLFAYLFRAKELGVDAREHTEVVGFEHEGDVITAVKFRSNGKVESVKVDAVLNAANAWAPLINEMAGLKRDLVPITAYKHQLVKTEPLERGQAEPLVCPPSWDDAYIIQDGEDGGIICGAGIEHRAKSLDDYEPTYDFLRGVLRYATMIAPPLRYAHIVRQWAGFYAKTPDKNPAIGKLLDNFYIAAGFSGHGFMMAPAVAQAMAELIAKGRSKVPLDWEWYDPYRFGRGELRSSAFQIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5C6</accession>
    <name>A0A218P5C6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Replication factor C small subunit</fullName>
            <shortName evidence="7">RFC small subunit</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">Clamp loader small subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rfcS</name>
        <name evidence="9" type="ORF">A3L08_00980</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heteromultimer composed of small subunits (RfcS) and large subunits (RfcL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the activator 1 small subunits family. RfcS subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853266.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5C6"/>
    <dbReference type="GeneID" id="33314800"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00980"/>
    <dbReference type="OrthoDB" id="7928at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000029">
        <property type="entry name" value="Replication factor C small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000012">
        <property type="entry name" value="Replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000129">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01509">
        <property type="entry name" value="RfcS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023748">
        <property type="entry name" value="Rep_factor-C_ssu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001679">
        <property type="entry name" value="PRK00440.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF20">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="45"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="53"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="326" mass="36941" checksum="1B99D62746A063CD" modified="2017-09-27" version="1">MSAEVQEVKILEKPWVEKYRPERLDDIVGQAHIVKRLKHYAKTGSMPHLLFAGPPGVGKTTAALALARELFGENWRHNFLELNASDERGINVIREKVKEFARTKPIGGASFKIIFLDEADALTQDAQQALRRTMEMFSNNVRFILSCNYSSKIIEPIQSRCAIFRFRPLNDDAIAERIRYIAENEGLELTEEGLQAILYVAEGDMRRAINVLQAAAALDTKITDENVFLVASRARPEDVREMMELALEGNFLKARDKLREILLKQGLSGEDVLIQMHKEVFNLPIPEDKKVALADKIGEYNFRLVEGANEMIQLEALLAQFTIMGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5F8</accession>
    <name>A0A218P5F8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Iron ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_01000</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853270.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5F8"/>
    <dbReference type="GeneID" id="33314804"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01000"/>
    <dbReference type="OrthoDB" id="57034at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06550">
        <property type="entry name" value="TM_ABC_iron-siderophores_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3470.10:FF:000001">
        <property type="entry name" value="Vitamin B12 ABC transporter permease BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="entry name" value="ABC transporter involved in vitamin B12 uptake, BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472:SF25">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE PROTEIN MJ0876-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="FERRIC ENTEROBACTIN TRANSPORT SYSTEM PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="ABC transporter involved in vitamin B12 uptake, BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="125"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="338" mass="36302" checksum="48552361B488EC2F" modified="2017-09-27" version="1">MRKWLPALITLSITSLFLGVYIGSVSISPSDITSSIAYGIKEAFHGLFPSIDPGEKPSYFVIVWEIRLPRVLLAYLVGLSLAGAGTASQALFRNPLADPYIMGISAGAGLGAALASVYWHGHLGLAALVSAILSVFIVYSVSRVDGKVPVDTLLLAGIAFGFLASAATWYILLSHQDRATLTWMWLMGSFNGATWGDVGKMVLVSALGLGFLVWKWRELNLVLLGEESIALGLDLHLYRRLFIGAITLLTAFSVSTSGIIGFVGLMGPHIMRLILGPNHRELTPASALFGGVLLVTADLIARVLSRPTEIPVGIVTAMMGAPFFLYLLMKHKRGELYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5I9</accession>
    <name>A0A218P5I9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rubrerythrin diiron-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853300.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5I9"/>
    <dbReference type="GeneID" id="33314839"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01175"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rr_diiron-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33531:SF10">
        <property type="entry name" value="BLR7895 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33531">
        <property type="entry name" value="RUBRERYTHRIN SUBFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rubrerythrin diiron-binding" evidence="1">
        <location>
            <begin position="23"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="162" mass="18624" checksum="B30B84E79C9ABF40" modified="2017-09-27" version="1">MATIDVDIIQDVEVLLRNLNGYELLSYAICNEECGAESYEWLSGRVEGILVDEFKHLAKEKRKQAAQIRELFKRLYPNMDPLKFNAPPLDTLPVCGEMMGVEDVEKALAIALLSEAIGKDVYRKLQRMTGDEETAELFGRLAEIKEEAYKRLLEFYETMIER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5K9</accession>
    <name>A0A218P5K9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_01440</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">tRNA modification; archaeosine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeosine synthase type 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853349.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5K9"/>
    <dbReference type="GeneID" id="33314893"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01440"/>
    <dbReference type="OrthoDB" id="115061at2157"/>
    <dbReference type="UniPathway" id="UPA00393"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002099">
        <property type="term" value="P:tRNA wobble guanine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21149">
        <property type="entry name" value="PUA_archaeosine_TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.90">
        <property type="entry name" value="ArcTGT, C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10630">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053418">
        <property type="entry name" value="Archaeosine_synthase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050076">
        <property type="entry name" value="ArchSynthase1/Queuine_TRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040777">
        <property type="entry name" value="DUF5591"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038250">
        <property type="entry name" value="TGT_C2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040592">
        <property type="entry name" value="tRNA_mod_ArcS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46499">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46499:SF1">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17884">
        <property type="entry name" value="DUF5591"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88802">
        <property type="entry name" value="Pre-PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="tRNA-guanine transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="PUA" evidence="4">
        <location>
            <begin position="502"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="568" mass="65157" checksum="8304EC0CD7169E56" modified="2017-09-27" version="1">MEVLRHEGPGRLGLVRLGEHSFRTPALAGVDFTLSPFNSFFHPDEPGDYDFNLAPSIPLGFYTPGEVIEKAIGRLWSVNYEGFNAFYLPALRRTEYLGEFFKIIERYGFEAVYLGNSKILVKEYRYFVKILRELRERFPNVMIIADLEPFFYPLAVYLGVDAFDTRSLKLYDFEGKGFTQFSPFIWSDEPNSLDFAKETILEVRKALEEGKLRYLVENFFHTQYHAGILRIADLEHPDYLEKYTPIQKETVYFISDASIRRPEVKRWHSRVEERFLPPKNTELVLLFPCSAKKPYSFSRSHTLYRKAMKEALGSGISKVHELILTSPFGVVPREWEWLAKYDIVVTGHWGEEEIKPAAELLAKTLEKYPKDVPIIAHLDEAYVEIARLASEMTGREIIFTEVKNGTTSRESLDSLTETLKEFQLEGTKEDRTYRYFENIRKVFDFYFGAGAGEAVLPENGQVKGSKMLRLFVDNQQTGTFKDGVISVTPFGMQRIYDALKSYWVKVDFDLRGDVFAVGVDEADERIRPDDIVGIVRDGKVIGVGKAVLAGEEMVNAKKGVAVKVRKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Q6</accession>
    <name>A0A218P5Q6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">S26 family signal peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_01640</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853383.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5Q6"/>
    <dbReference type="GeneID" id="33314933"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01640"/>
    <dbReference type="OrthoDB" id="4822at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001733">
        <property type="entry name" value="Peptidase_S26B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02228">
        <property type="entry name" value="sigpep_I_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806:SF6">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00728">
        <property type="entry name" value="SIGNALPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="158" mass="17870" checksum="E3AC94F93C25BD93" modified="2017-09-27" version="1">MDEGWKKDLTWALIAVIVVFIFQFGLKVALHTDSPLVIVVSGSMEPVFYRGDVVLLKGINEDNIDDVHVNDVIVYKRPGYEYPIIHRVREISLVKLGNKTEKCFLTWGDNNWAPDPDYPTPYGMVPCVPAYAVEDKALLVFPKIGLIPLEIREWLGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5R3</accession>
    <name>A0A218P5R3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01530</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853365.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5R3"/>
    <dbReference type="GeneID" id="33314911"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01530"/>
    <dbReference type="OrthoDB" id="84640at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="117" mass="13849" checksum="C496F52272E3B0CC" modified="2017-09-27" version="1">MSAREMRMEMFLKALLKRDFSRAKGHLEKLQKMAGSDEWGRGYSKAINGFMSAIKDNDPDALIVQLITEHNREKAEELLRHFQDVILEHEFRDDYEKGYYTAWVEFLRAYLSQKTLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5V4</accession>
    <name>A0A218P5V4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MrpA C-terminal/MbhD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_01750</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853403.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5V4"/>
    <dbReference type="GeneID" id="33314955"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01750"/>
    <dbReference type="OrthoDB" id="102564at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025383">
        <property type="entry name" value="MrpA_C/MbhD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006242">
        <property type="entry name" value="PRK08378.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13244">
        <property type="entry name" value="MbhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="MrpA C-terminal/MbhD" evidence="7">
        <location>
            <begin position="17"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="87" mass="9262" checksum="D0F176110646C9AB" modified="2017-09-27" version="1">MNALTLDMTIQAIILLGVLITAYLTIRSRDLLVAALMSAAMSLLLSLEFYSLHAPDVAIAEAAVGAGVVTALVVYGIAKTERWEGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5X4</accession>
    <name>A0A218P5X4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Molybdopterin synthase sulfur carrier subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_01935</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853438.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5X4"/>
    <dbReference type="GeneID" id="33314992"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01935"/>
    <dbReference type="OrthoDB" id="98357at2157"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990133">
        <property type="term" value="C:molybdopterin adenylyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00754">
        <property type="entry name" value="Ubl_MoaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000010">
        <property type="entry name" value="Molybdopterin synthase sulfur carrier subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010038">
        <property type="entry name" value="MoaD_arc-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044672">
        <property type="entry name" value="MOCS2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054834">
        <property type="entry name" value="SAMP1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01687">
        <property type="entry name" value="moaD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041918">
        <property type="entry name" value="SAMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33359">
        <property type="entry name" value="MOLYBDOPTERIN SYNTHASE SULFUR CARRIER SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33359:SF1">
        <property type="entry name" value="MOLYBDOPTERIN SYNTHASE SULFUR CARRIER SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="88" mass="9915" checksum="25FCE91F1906272A" modified="2017-09-27" version="1">MKVTVRYFARYRSLVGKGEEELEVPDGITVGELIEILREKHPVLRNEVFAEDDDLADVNVSRNGRYVRFDEVLHDGDVVALFPPVSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Y1</accession>
    <name>A0A218P5Y1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_02045</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853458.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5Y1"/>
    <dbReference type="GeneID" id="33315014"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02045"/>
    <dbReference type="OrthoDB" id="49941at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007417">
        <property type="entry name" value="DUF473"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04322">
        <property type="entry name" value="DUF473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="127" mass="14368" checksum="C064214CC272AB16" modified="2017-09-27" version="1">MEAVVLAGMARRVLDELMRNPYRTIELRSARNVLAVEEALERAMRLFLTYDPFSDVDVGTEGLLAELLGAEKLETRVPWEESDEREVTVCRAKVKLVGLGRVVEVENKKGLLIVRIRELIPQEMGMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Z2</accession>
    <name>A0A218P5Z2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">5-methylthioadenosine/S-adenosylhomocysteine deaminase</fullName>
            <shortName evidence="4">MTA/SAH deaminase</shortName>
            <ecNumber evidence="4">3.5.4.28</ecNumber>
            <ecNumber evidence="4">3.5.4.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mtaD</name>
        <name evidence="6" type="ORF">A3L08_02060</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the deamination of 5-methylthioadenosine and S-adenosyl-L-homocysteine into 5-methylthioinosine and S-inosyl-L-homocysteine, respectively. Is also able to deaminate adenosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>S-adenosyl-L-homocysteine + H2O + H(+) = S-inosyl-L-homocysteine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:20716"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:57985"/>
            <dbReference type="EC" id="3.5.4.28"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>S-methyl-5'-thioadenosine + H2O + H(+) = S-methyl-5'-thioinosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:25025"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:48595"/>
            <dbReference type="EC" id="3.5.4.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the metallo-dependent hydrolases superfamily. MTA/SAH deaminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.28" evidence="4"/>
    <dbReference type="EC" id="3.5.4.31" evidence="4"/>
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    <dbReference type="KEGG" id="tpaf:A3L08_02060"/>
    <dbReference type="OrthoDB" id="372084at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090614">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:S-adenosylhomocysteine deaminase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Urease, subunit C, domain 1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AMIDOHYDROLASE-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="5">
        <location>
            <begin position="51"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>substrate</name>
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        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
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        <location>
            <position position="211"/>
        </location>
        <ligand>
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        </ligand>
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    <feature type="binding site" evidence="4">
        <location>
            <position position="296"/>
        </location>
        <ligand>
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        </ligand>
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    <feature type="binding site" evidence="4">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="424" mass="46406" checksum="22DA6FE981700162" modified="2017-09-27" version="1">MSVLIRNGHVIYGEDFEIVKADVLIEGNRIAKVAKGINEGADTVIDAKGRVVSPAFVNLHTHSPMGLFRGLADDLPLMDWLQNHIWPREAKLTREYTKVGAYLGALEMIKSGTGTFLDMYFFMDAVAEVVEESGLRGYLSYGMIDLGDPEKTEKELKEALRTMEFIEKLNSDRVQFVFGPHAPYTCSIALLKEVRRLASEHNKLITIHVSETMAEIGQITERYGKSPVVLLDDIGFLGRDVIIAHGVWLDSRDIQILARNGVTVAHNPGSNMKLASGVMPLQKLLNAGVNIGLGTDGAASNNNLDMLDEMKLAALLHKVHNLDPTVADAETVFRMATLNGAKALGINAGAIKEGYLADIAIIDFNQPHLRPVNNVISHLVYSANGNDVETTIVNGEILMLDGEILTLDEEKVIAEAERVSEKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Z7</accession>
    <name>A0A218P5Z7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TIGR04076 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_02165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06216.1"/>
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    </dbReference>
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        <property type="entry name" value="CHP04076"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="111" mass="12112" checksum="FBF3B31A3867DB2C" modified="2017-09-27" version="1">MEKLEARIVEIRGKCPVFRLGDRVTIEGPRINLKETDAVCTHAFASLLPYIVALRKGIKPEELGLGRGEKAYVQCLDPGPPYTDGGTVIFEITVVRDEGEKGLESGEGGNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P606</accession>
    <name>A0A218P606_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein-glutamine gamma-glutamyltransferase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_02205</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853487.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="tpaf:A3L08_02205"/>
    <dbReference type="OrthoDB" id="99436at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="entry name" value="TgpA-like_C"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Protein-glutamine gamma-glutamyltransferase-like C-terminal" evidence="2">
        <location>
            <begin position="207"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="283" mass="32245" checksum="E8123338627DE1B3" modified="2017-09-27" version="1">MSTRVKFAALYGLLFALMTLLLSYSVKTGEVKKSPAEFSGFLVIAVALLSAIVLALLFLSWRDIPLKRKLYPANNLKYLLSWIVMALGALGIIYWMKYSNASGLLTPVNNTTNVTVNGSVLPPSPVYHNNTLTNSTSPLSHTGYPLYIFLAVFMAGLAYFAVTYYREALKKRERKRMRKRALNFDRKVDELGFEMFSDPREAVVGIYKNAVLWLEVLGIPYRESWTHWEHAGKVNVSLLREPFTNLTRLFEKAKYAPEKVTWDDAERALELYRKMRGVLNEAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P610</accession>
    <name>A0A218P610_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_02255</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853496.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P610"/>
    <dbReference type="GeneID" id="33315056"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02255"/>
    <dbReference type="OrthoDB" id="92146at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="108" mass="12480" checksum="7B82815A56E1692D" modified="2017-09-27" version="1">MKMRVVFDKEYGLLSGVYMVRVRELEFDEELKEVLDGVDPAIRIRGEEVKLSELTERSFELQTREEAERTMGNVRVALIEALSALIARFKEAQSFNGSVSYEIDFNEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P617</accession>
    <name>A0A218P617_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Carbohydrate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_02230</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853491.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P617"/>
    <dbReference type="GeneID" id="33315051"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02230"/>
    <dbReference type="OrthoDB" id="26949at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002139">
        <property type="entry name" value="Ribo/fructo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10584:SF166">
        <property type="entry name" value="RIBOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10584">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00990">
        <property type="entry name" value="RIBOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="5">
        <location>
            <begin position="5"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="277" mass="30359" checksum="D5B8072F9C0FF415" modified="2017-09-27" version="1">MGFDLVVLGHVSIDHIRMPGKPEVILPGGAAAAVATSAALAGAKVGLVTKVGEDFPKEWLEKLASLLDVRGVHVLPGKTIHIYMIYHEDGSVDAPVDMGVARMMGETPIPEEYLDSEVFHIAPIPPEEQLKALKRLDGKRISLDFNPTYMGDYLSRTDLMREIVSRVDVLFPNEREALVITKAKTVEEAAERLHEWGAKLVVVTRGERGVLLYDGEFREFEALPISENEIVDPTGAGDAFAGGFLAGYSRGKPLEECVRLGLERAREVLKKEGSWSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P622</accession>
    <name>A0A218P622_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MoxR associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_02220</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_232461777.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P622"/>
    <dbReference type="GeneID" id="33315049"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02220"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002881">
        <property type="entry name" value="DUF58"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33608">
        <property type="entry name" value="BLL2464 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33608:SF6">
        <property type="entry name" value="BLL2464 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01882">
        <property type="entry name" value="DUF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="DUF58" evidence="2">
        <location>
            <begin position="209"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="423" mass="48450" checksum="1F46A31F9A26B934" modified="2017-09-27" version="1">MNAGGPDEKGPVEGRMIPTEKTEELLLAVWLLVLIAFLLLRWEMVYLLLPALWLVFVAVFFFKPRLDVELERIIPHNRFLEGSELEVVLRIKSKERIPSLKIREDLPEELELIEGEREWVVSLSKGETREFRYKVRVKRGIHEFNWVELSYRDPFGFFHFTKKFDLYTEVIGVPIIEDVPTPYSTRGTKITVGALPSPRVGEGVEFHAIREYQPGDPLKIINWKATARTGRIMANEYESERKVDVIFIVDASYTGELVFDNLIRAAASLMLNALNSGTSFGLLLAEDAPLWVRPDYGKRHFFKCIDFLSTARPDRNNMIAYQVEHLIRSHFPPRAQLVYFSPLLTEESREALKIMASFGYNVAVISPDPYTAVEPKSREEELALKLLSLQRKAALKKMAAYGIIIDWDVRKPLKAAIAEVVGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P623</accession>
    <name>A0A218P623_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853465.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P623"/>
    <dbReference type="GeneID" id="33315021"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02080"/>
    <dbReference type="OrthoDB" id="102548at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="59" mass="6774" checksum="8DD116B0B2CBDDB8" modified="2017-09-27" version="1">MLFVFGFLVLYYAIRKVACAQGFCDSSFFHRTPPVYSADMLAMVFSVSVLFFALLWYVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P646</accession>
    <name>A0A218P646_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FAD-dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853524.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P646"/>
    <dbReference type="GeneID" id="33315089"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02420"/>
    <dbReference type="OrthoDB" id="4240at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028348">
        <property type="entry name" value="FAD-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049516">
        <property type="entry name" value="FAD-depend_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43106">
        <property type="entry name" value="DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43106:SF1">
        <property type="entry name" value="DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21688">
        <property type="entry name" value="FAD-depend_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01946">
        <property type="entry name" value="Thi4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038984">
        <property type="entry name" value="FAD_binding_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-dependent protein C-terminal" evidence="1">
        <location>
            <begin position="265"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="488" mass="54124" checksum="346CF3C7CA166E7E" modified="2017-09-27" version="1">MVSDNGSGKVYDVVIIGAGPAGLFAAYELAERSDFKILVIDEGGDVDQRKCPMYELGHCIGCQPCHIMSGVGGAGGLSDGTINLRPDIGGDLRELTNDENYAWQLVWEVDQIFLRHKAPRNLFKGDSEQVRYWEQKAAQAGVKFIPIIQRHIGSDRTPEVIGNIKRHLESKGVKFLLWTKALEFGRGWVKVKRGKDVFEIKAKYIIVAPGRGGAEWFHDVAQKIGLKARHGPIDVGVRVEVPAIVMEPITSINHDPKFHIYTDTYDDFVRTFCTNPNGFVVEERYDGYVGVNGHSMHGKKSNNTNFAFLSRIELTEPVEDTTAYGRSIAQLATTIGGGRPIIQRLGDLRRGRRSTWARIRRSDVEPTLKHVTPGDIAMALPHRVVTNIIEGLEKLDRVLPGVASDHTLLYAPEIKYYAMRAEVNENLETSIEGIFAAGDGAGLSRDIVNAAATGILAARGILKKEGLYTEREFRKPGNWRHAIESLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P667</accession>
    <name>A0A218P667_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">4-aminobutyrate aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_02450</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853530.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P667"/>
    <dbReference type="GeneID" id="33315095"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02450"/>
    <dbReference type="OrthoDB" id="6534at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004171">
        <property type="entry name" value="PRK05639.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF58">
        <property type="entry name" value="LEUCINE_METHIONINE RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="459" mass="50268" checksum="B0E4887ED02A8149" modified="2017-09-27" version="1">MTSEYPKIVVKPPGPKARKMIEREKRVVSSGLGVKLFPVVPERGYGALIEDVDGNVFIDFLAGAAAASTGYAHPRLVKEVQEQVAKIQHSMIGYTYSKRAIEVAEILAERAPVDSPKILFGLSGSDALDLTMKVARFATRKPWIVAFIGAYHGQTYGATSIAAFQSSQKRGFSPLVPNVVWVPYPNPYRNVWGIDGYEEPDELINHFLDYMESYIFAHVVPPDETAVLIAEPIQGDAGIVVPPENFFVELKKLLDEHGILLAMDEVQTGIGRTGRWFASEWFGVRPDLVAFGKGVASGMGMSGVIGRGELMEMTSGSALLTPAANPVISAAAYATLRIIEEEDLLGNALRVGEFIQKRLREMQEEYEVIGDVRGKGLMIGAEIVKPDGKPDPELTGKICWRAFELGLILPSYGMFGNVIRITPPLVITEELAEKGVEIMETALKDALAGRVTHRTVTWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6A0</accession>
    <name>A0A218P6A0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hded protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853542.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6A0"/>
    <dbReference type="GeneID" id="33315107"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02510"/>
    <dbReference type="OrthoDB" id="101111at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052712">
        <property type="entry name" value="Acid_resist_chaperone_HdeD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005325">
        <property type="entry name" value="DUF308_memb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34989">
        <property type="entry name" value="PROTEIN HDED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34989:SF1">
        <property type="entry name" value="PROTEIN HDED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03729">
        <property type="entry name" value="DUF308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="188" mass="20281" checksum="A8BFBE3DCFA9C932" modified="2017-09-27" version="1">MEYGEVKKNWIWMLGLGIIFTTLGFAGLMILPLLTITSVAIFGAFMVVGGALQVIQGITKTKDWKSRTLHIIMGAIYVIGGIATLENPVLATTILTLVLGFSLITIGAIRIGVALQNKDISQWALMTLSGFLTILLGFMIVIQWPWSGLWAVGLFVSVDLIMSGLNFIAIALSAKMEHTQGQKNPSTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6A2</accession>
    <name>A0A218P6A2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_02735</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853585.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6A2"/>
    <dbReference type="GeneID" id="33315153"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02735"/>
    <dbReference type="OrthoDB" id="27015at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01124">
        <property type="entry name" value="KaiC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010624">
        <property type="entry name" value="KaiC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637:SF1">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51146">
        <property type="entry name" value="KAIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KaiC" evidence="3">
        <location>
            <begin position="8"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="249" mass="28191" checksum="223B5E6222FD4B80" modified="2017-09-27" version="1">MVTEYTVERVKSGIQGFDDLIEGGFPKGTTVLVTGPTGSGKTTFGVQFVYKGAELYDEPGVIVTLEERSRDLRREMLAFGWDIGKYEREGKIAIIDGVSAVVGLPSEEQYVLEGNLNAEDFLRYVYRVVKAINAKRLVIDSIPSIAFRLKKEEEIRSVLLQFNTILLEMGVTSILTTEAPDPSRGKISRYGIEEYIARGVILLDFVEKEVELKRYLLIRKMRETKHSMKKYPFEINENGIVVYPSGELY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6B0</accession>
    <name>A0A218P6B0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853595.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6B0"/>
    <dbReference type="GeneID" id="33315163"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02785"/>
    <dbReference type="OrthoDB" id="73420at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07741">
        <property type="entry name" value="metallo-hydrolase-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42663:SF6">
        <property type="entry name" value="HYDROLASE C777.06C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42663">
        <property type="entry name" value="HYDROLASE C777.06C-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="32"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="271" mass="30460" checksum="52D3AE8668E1B205" modified="2017-09-27" version="1">MIELTFLGSGGGRFITITQFRSTGGFHIRASRNIYVDPGPGALVRSWRYKLDPRKLDAIFVSHRHVDHCNDLEVMVEAMTGGALKKRGTLIASKSVVYGDETHTPAISKYHLDVLESVHIPEPGSRIPIGEDEFIITPSQHSDPTTIGFRMKTRFGDISYIPDTAYFDGLIEWHDGARLLIAAVTRPRDMGIPYHLSTDDAVEMLKSMEKKPEALVISHIGMKMHFANPYKEAKYIENVTGVKTYVAKEGFRVMVEKNEISVRTLRPARFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6D4</accession>
    <name>A0A218P6D4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Peptide ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_02620</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853563.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6D4"/>
    <dbReference type="GeneID" id="33315130"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02620"/>
    <dbReference type="OrthoDB" id="312811at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050366">
        <property type="entry name" value="BP-dependent_transpt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43386:SF1">
        <property type="entry name" value="D,D-DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DDPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43386">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN APPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="342"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="384"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="214"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="422" mass="46592" checksum="8DE255A419B829C7" modified="2017-09-27" version="1">MRTNKLRLGVAIIVIYTIAAFIAPYFVNEEKIENWYNGLYWKENPKLAPPSWVNLFGKSLPPTEELAPVKSQSGLQAFEYDFRYSKPPQDIIVKSNKTEGSPMTVTVTTPSGESYQFYRGISDEVSLAYQWQVLMEIAEDRGVEFRMEDTAFRMGLKPLFFVNQSGEVVPEHGVYTITVEGALNSTVKIVGATYGILGTDTYGRDVGAAFLWGARQTVVLVYLTAVVAVVLGTLAGLAASLRGKTGTVVDSISKLSTIVPVIPFMIALTPLTGSVSYNARITVPMWAFVLALAVLLFGKIARNVGAIVRTELNKEYVAAAESLGADRKWILRRHILKIVGPYSIYQLSLFTPKAIALISILGFFRVAPGFNWGTMLGMVLSQNAIYSMAWWMIVPIGLALVVFALAFVLINRELEERFLSRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6E7</accession>
    <name>A0A218P6E7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA primase small subunit PriS</fullName>
            <ecNumber evidence="11">2.7.7.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">priS</name>
        <name evidence="14" type="ORF">A3L08_02725</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalytic subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. The small subunit contains the primase catalytic core and has DNA synthesis activity on its own. Binding to the large subunit stabilizes and modulates the activity, increasing the rate of DNA synthesis while decreasing the length of the DNA fragments, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair.</text>
    </comment>
    <comment type="function">
        <text evidence="13">RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterodimer of a small subunit (PriS) and a large subunit (PriL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11 12">Belongs to the eukaryotic-type primase small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853583.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6E7"/>
    <dbReference type="GeneID" id="33315151"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02725"/>
    <dbReference type="OrthoDB" id="31125at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04860">
        <property type="entry name" value="AE_Prim_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.160">
        <property type="entry name" value="DNA primase S, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.920.10">
        <property type="entry name" value="DNA primase, PRIM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00700">
        <property type="entry name" value="DNA_primase_sml_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002755">
        <property type="entry name" value="DNA_primase_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014052">
        <property type="entry name" value="DNA_primase_ssu_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023639">
        <property type="entry name" value="DNA_primase_ssu_PriS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00335">
        <property type="entry name" value="primase_sml"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10536">
        <property type="entry name" value="DNA PRIMASE SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01896">
        <property type="entry name" value="DNA_primase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56747">
        <property type="entry name" value="Prim-pol domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 11 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 11" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3 11 12" id="KW-0639">Primosome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 11 12" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="11">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ06321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="346" mass="40713" checksum="AF8C2C50A117BC4B" modified="2017-09-27" version="1">MSELFREATKEERVRYYRREWSAKRLPEFIVETLENREFGFDHTGEGPSDRKNVFLDVRDLEDYMKATAPYAVYSSVALYEEPREMEGWLGAELVFDIDAKDLPLRRCQHIHEHGRVCPICLEDAKELARDTLIVLKEDFGFESVHVIYSGRGYHIRVLDDWAMTLDGKAREKVLSYISAAEEITFDDIRSRRIMLSSGYYRVFRLRFGYFLRRMNENHLVNINLRRGQVEKLLGNREEVYENFVRKGLLTAFPQGIGYKTLTKLFALSSTFSKAYFDGRVTVDVKRILRLPSSLHSKVGFITTYIGPDEGKLERFNPFEDAVPRFRREEVREAYRLWLEEHGDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6F6</accession>
    <name>A0A218P6F6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PqqD family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_02775</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853593.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6F6"/>
    <dbReference type="GeneID" id="33315161"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02775"/>
    <dbReference type="OrthoDB" id="211309at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1150">
        <property type="entry name" value="Coenzyme PQQ synthesis protein D (PqqD)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008792">
        <property type="entry name" value="PQQD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041881">
        <property type="entry name" value="PqqD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05402">
        <property type="entry name" value="PqqD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="13240" checksum="55BEBE0B6251D4C2" modified="2017-09-27" version="1">MEEYLNLIPVCNEKVQLRKIEGKYYLLVPMESKLDFLARKLHGDYRRIELDEVGAYIWELCDGRRTIGEIGKALKARFGEEVEPLYERLVTFLLELRKRYLVEFKRADDIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6I6</accession>
    <name>A0A218P6I6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PIN domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853656.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6I6"/>
    <dbReference type="GeneID" id="33315235"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03145"/>
    <dbReference type="OrthoDB" id="89389at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002850">
        <property type="entry name" value="PIN_toxin-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00305">
        <property type="entry name" value="putative toxin-antitoxin system toxin component, PIN family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34610">
        <property type="entry name" value="SSL7007 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34610:SF3">
        <property type="entry name" value="SSL7007 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13470">
        <property type="entry name" value="PIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="146" mass="16672" checksum="631B34AF19171CED" modified="2017-09-27" version="1">MKVVMDTNVVLAAMIKPSGLAALLIKALDGNFLVNYTSEEALHELSLKIGLLAEKGRLFSEWKRILARYLRGSITVSPSREFNLSRDPNDNKWLEIAYEGKVEYILTWDDDLIALRGENRVVCLEDHTLKILPPLEFYHEVLNQIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6K2</accession>
    <name>A0A218P6K2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycosyl transferase family 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853651.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6K2"/>
    <dbReference type="GeneID" id="33315226"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03100"/>
    <dbReference type="OrthoDB" id="132546at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050194">
        <property type="entry name" value="Glycosyltransferase_grp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947:SF3">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="1">
        <location>
            <begin position="144"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="356" mass="41151" checksum="D1DE277492B7585E" modified="2017-09-27" version="1">MRIAVIGHFDNTDEGTRIVAKTIAQKLEERGLRVKKINISSISTFLEIRRFKPDVIHFVIGPTTLGFISTKLLSFSWLRAVTVLSVVHSSIPKKKILSLFRHDIALVQSKDSERVYEELGFNTRFLPNGVDIDKFKPVPIEQKIKLREKYGLPTEKFIILHMASMKRKRNLSVFKTLKQQFSDIEILLIGRENEKNIDWELVKELKSAGCRVWLKHFPEIEEIYQLADCYVFPAKNKTAAIETPLSVLEAMACNIPVITTKFGALPRMFDEGEGLFFVENEDEIVSIIKTLREKRDIPVNTRKKVLKYSWDNIINDLIKIYNRMLGEGYEKTDHNSISWSRRFGKKHSIQGNSPRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6K7</accession>
    <name>A0A218P6K7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2341 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03305</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_157721581.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6K7"/>
    <dbReference type="GeneID" id="33315267"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03305"/>
    <dbReference type="OrthoDB" id="101984at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1072" mass="120955" checksum="CA36A2497B883D9D" modified="2017-09-27" version="1">MKRRGFLINSAVLLLLIPLLLLIATYEDASSMIITSQSENVQIERTFRLTSYLEEDFKNTLSLSTKRAIALSVDYVTSERPLDNASAALKQLITYGHYPYIGGTNSEWKSREEFFMKNNTIKDWLRNMEWELERQGYTMKPSPDEIVQNMKLTVAPLDSFHIVVNASIPNIIIEDSSGLVVYNSSIPQKGSVYVVIPIEGIEDPLFPHLTSGRTSRIISACKFAYPSITPPYTRLDGYGHSSIKTFSGQLYNVPRGGTIFYSDKYTAGENVLGYITRQQPSETPNAPYIFNTTLGGRKVSPLSVFNPGDIGVMTFDSISGGTGTPSHWCEKKLEYRANMTLPSTAPPNSLVLLELTPSSVPFGSAVHDGSAASIRIYKRSDTSCEIAPYWIEYWGDDKILIWLNTTDTREYTVYYSTSDQSMEWSGNIAIFPVHNQSVALTAGEEESKLVSTVPWDSFFVRYSVKASTSTWDFDSGVEVETIPKGGEKYLKATVNYPESLSGVQIPIHLDSATAQAITHNSQNEAQIEVYSDEQLQNPVPFWIEYWNDNGALIWVKGNLPGTFYIKYNTGTYTRGDGSQVFLWFTDSDKRIDDGQSTSFDLSSYGIQGDIAIRFSMKPTTKNKAWNAGIRVHTEYTYKVRGRWYTDVYYINFTDDLVEEDNTLKIQDEWWDDYYGGWYSYYPTSVQKTRGCCGYRTYEVSIHPGYWDGDYPVADVDFADYGTTNRAYFDNPIRYNDDDGYYRVYKDPLLSLELINLDDNNDNTAVFDWVFIRRYVDISQLSEYVEIAGGQEPVSLQFIDDNPGHQDHGGDKLAILQDWDTNLDNYNGAWDVETPQRYEVIVEKDSINLDLTFTHSPNLAGSRESTASVQIGQVTGFKLFAIIDNGQGNDAYFDWIVAALYPYETYTESQITTTSSESVPSAGGYSTARAYDIQPFIDCIQAQKYFGVQGAPSFFERLEGGDTTNRNYYERIAAKMQMAVYGTARYPIGLVSFILPKDLPPNLNFLIRRQPAADYIYLNYRDYPSDNPNAKKVFGISTNGGVSSPLLDENFYLTPAIARKMFDVQGASDLLQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6L4</accession>
    <name>A0A218P6L4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphoribosylformylglycinamidine synthase subunit PurS</fullName>
            <shortName evidence="6">FGAM synthase</shortName>
            <ecNumber evidence="6">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Formylglycinamide ribonucleotide amidotransferase subunit III</fullName>
            <shortName evidence="6">FGAR amidotransferase III</shortName>
            <shortName evidence="6">FGAR-AT III</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Phosphoribosylformylglycinamidine synthase subunit III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">purS</name>
        <name evidence="7" type="ORF">A3L08_03265</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PurS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853677.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6L4"/>
    <dbReference type="GeneID" id="33315259"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03265"/>
    <dbReference type="OrthoDB" id="56303at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1280.10">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase subunit PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01926">
        <property type="entry name" value="PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003850">
        <property type="entry name" value="PurS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036604">
        <property type="entry name" value="PurS-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00302">
        <property type="entry name" value="phosphoribosylformylglycinamidine synthase subunit PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004630">
        <property type="entry name" value="PRK05974.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34696">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34696:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02700">
        <property type="entry name" value="PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82697">
        <property type="entry name" value="PurS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="80" mass="9443" checksum="9583CA20531CE0B8" modified="2017-09-27" version="1">MKWKVKVIVRLKEGLNDPEGRVIGKALRNLGYAVENLRVPKYFEFELESENPEEEVEEMCRKLLANPLIHSWEYRVEPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6N5</accession>
    <name>A0A218P6N5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase</fullName>
            <ecNumber evidence="10">6.3.4.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate--formate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">purP</name>
        <name evidence="13" type="ORF">A3L08_03280</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the ATP- and formate-dependent formylation of 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'-monophosphate (AICAR) to 5-formaminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'-monophosphate (FAICAR) in the absence of folates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + formate + ATP = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24836"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (formate route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.23" evidence="10"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853679.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6N5"/>
    <dbReference type="GeneID" id="33315262"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03280"/>
    <dbReference type="OrthoDB" id="98133at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00134"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01163">
        <property type="entry name" value="IMP_biosynth_PurP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023656">
        <property type="entry name" value="IMP_biosynth_PurP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009720">
        <property type="entry name" value="IMP_biosynth_PurP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010672">
        <property type="entry name" value="IMP_biosynth_PurP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009779">
        <property type="entry name" value="PRK13278.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38147:SF2">
        <property type="entry name" value="5-FORMAMINOIMIDAZOLE-4-CARBOXAMIDE-1-(BETA)-D-RIBOFURANOSYL 5'-MONOPHOSPHATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38147">
        <property type="entry name" value="5-FORMAMINOIMIDAZOLE-4-CARBOXAMIDE-1-(BETA)-D-RIBOFURANOSYL 5'-MONOPHOSPHATE SYNTHETASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06849">
        <property type="entry name" value="DUF1246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06973">
        <property type="entry name" value="DUF1297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004602">
        <property type="entry name" value="ATPgrasp_PurP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="12">
        <location>
            <begin position="98"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</name>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</name>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</name>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="331" mass="37660" checksum="A0FDDF7602A5613B" modified="2017-09-27" version="1">MRVATYASHSALQILKGAKDEGFETVAFGSGRVRPLYTKYFPVADHFIEGTYPEEELLELEAVVVPTGSFVAHLGIELVERMRVPYYGNKEVLRWESDRNLERRWLEKAKLRLPRVYDDPDDIDGPVIVKPHGAKGGKGYFLAKNPADFWEKAKRLGIRDKEDLSNVQIQEYVLGVPVYPHYFYSKLNRELELMSVDRRYESNADAIGRIPAKEQLGLELETNYTVIGNIPIVLRESLLMDVIEAGERVVKTAEELMGGLWGPFCLEGVFTEELEFVVFEISARIVAGTNPFVHGSPYSWLRYDFPVSTGRRIAMELRQALEEGRLGEILT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Q4</accession>
    <name>A0A218P6Q4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Preprotein translocase subunit SecG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">Protein transport protein Sec61 subunit beta homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">secG</name>
        <name evidence="14" type="ORF">A3L08_03580</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in protein export. The function of the beta subunit is unknown, but it may be involved in stabilization of the trimeric complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the protein translocase complex. Heterotrimer consisting of alpha (SecY), beta (SecG) and gamma (SecE) subunits. Can form oligomers of the heterotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the SEC61-beta family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853730.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Q4"/>
    <dbReference type="GeneID" id="33315321"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03580"/>
    <dbReference type="OrthoDB" id="43651at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00751">
        <property type="entry name" value="SecG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023531">
        <property type="entry name" value="Preprot_translocase_SecG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016482">
        <property type="entry name" value="SecG/Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002318">
        <property type="entry name" value="PRK01253.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03911">
        <property type="entry name" value="Sec61_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="6 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="12">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ06472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="56" mass="6058" checksum="935B5C4DA25E5B99" modified="2017-09-27" version="1">MAKEKTTLPPTGAGLMRFFDEDTKAIKIGPKGVIALTLILVAFEILLHTFGPQIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6R4</accession>
    <name>A0A218P6R4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_03450</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853709.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6R4"/>
    <dbReference type="GeneID" id="33315296"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03450"/>
    <dbReference type="OrthoDB" id="85833at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051797">
        <property type="entry name" value="TrmB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293:SF1">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="2">
        <location>
            <begin position="12"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="84"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="113" mass="13168" checksum="78ED40CAE64417DA" modified="2017-09-27" version="1">MSDVYERLEALLRSLGVKKTELKIYRLLLEKKQPMRITEIQRELGISERSVREHVLSLYRKGILRRTLIEQGWLGYTYSAVSPGEVLDNIKRNLLKRINELEQELKGSRAGEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6R8</accession>
    <name>A0A218P6R8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Exonuclease SbcD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853710.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6R8"/>
    <dbReference type="GeneID" id="33315297"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03455"/>
    <dbReference type="OrthoDB" id="10013at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024173">
        <property type="entry name" value="Pesterase_MJ0037-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39323">
        <property type="entry name" value="BLR1149 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39323:SF1">
        <property type="entry name" value="BLR1149 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000887">
        <property type="entry name" value="Pesterase_MJ0037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="20"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="216" mass="24446" checksum="06BE1B6D33DF83B9" modified="2017-09-27" version="1">MDSFDDLERLSLEMETSHGKTLLIADPHIGFELSRGLRIRTHFEERLAAFVSEKDPDLLVILGDVKEPIGLSFTVKRLLMGFFSDLREIPTVITKGNHDGRIEEVAGKFPHVQVTEHLLLDEELFLHGHTNLQGVEFREAYLGHIHPAYTFKSGGVSRKTKVFVRAGKFLILPTVNPFIEGFDVREGVRMVPFLRGVPSLDLFLPEGVYVGRVQLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6T9</accession>
    <name>A0A218P6T9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03765</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853763.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6T9"/>
    <dbReference type="GeneID" id="33315358"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03765"/>
    <dbReference type="OrthoDB" id="84697at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="73" mass="8618" checksum="8154ED67B2431038" modified="2017-09-27" version="1">MYMAEFKLRFGDRKWYVRRIVEASSMEEAEEKARRYAALMNRGEVKWELSYVIESERPLLIGDDELKKLASGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6V8</accession>
    <name>A0A218P6V8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6V8"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03805"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="68" mass="7560" checksum="BE7B0C256985EC83" modified="2017-09-27" version="1">MKESTGEVEITFPSQTGALVVVTLYGNRGFAETLKSAVEACRQSENVEERLISSRRIPSRGQSFPREK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6W1</accession>
    <name>A0A218P6W1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03740</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853758.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6W1"/>
    <dbReference type="GeneID" id="33315353"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03740"/>
    <dbReference type="OrthoDB" id="94677at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3490.10">
        <property type="entry name" value="PH0156-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10620">
        <property type="entry name" value="PH0156-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024508">
        <property type="entry name" value="DUF3226"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11536">
        <property type="entry name" value="DUF3226"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160945">
        <property type="entry name" value="PH0156-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="345" mass="39687" checksum="2F81D046E9ADC0EA" modified="2017-09-27" version="1">MRVVTGSRWEEFAEENSALFPEYRKNRDELLTFTSSLKGNETVVTSSLELIDLIAWRFRKGEENVLIYSDTGKALTLKEVYELRKYLDFDVRGGFSGEEARTSVLFVEGKTDAKFFKAVFKKLFEFKESRSAPRNLRFIERVFERDNFDLLRREGDNYYLAVIPSEGNSGVIRNLGNFLRAMDVFDFRVDRVGAAIDVDEDRESALASITGKLSGFKHEKTPLGYRVGETEVVPLIIGLPFEDELIEWKKPTVEDLMLHLIAREGLLDKVKPGLRALNESLGRKLKPKEVMYLALSAYGHWGNLEGFYELFVMRSRFRNLKAVLREARLMEELSYLAGMEVGGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Y7</accession>
    <name>A0A218P6Y7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rpl4lp</name>
        <name evidence="7" type="synonym">rpl4</name>
        <name evidence="8" type="ORF">A3L08_04050</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Forms part of the polypeptide exit tunnel.</text>
    </comment>
    <comment type="function">
        <text evidence="7">One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853816.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Y7"/>
    <dbReference type="GeneID" id="33315414"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04050"/>
    <dbReference type="OrthoDB" id="10737at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1370.10:FF:000011">
        <property type="entry name" value="50S ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01328_A">
        <property type="entry name" value="Ribosomal_uL4_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_uL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_uL4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013000">
        <property type="entry name" value="Ribosomal_uL4_euk/arc_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045240">
        <property type="entry name" value="Ribosomal_uL4_euk/arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019970">
        <property type="entry name" value="Ribosomall_uL4-arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03672">
        <property type="entry name" value="rpl4p_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19431">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="Ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00939">
        <property type="entry name" value="RIBOSOMAL_L1E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="255" mass="28672" checksum="6EF52F1A80E0B2AA" modified="2017-09-27" version="1">MKVKVFNLEGEPVEEIELPKVFSTPYRPDLIRRAVIASWTHRIQPQGRSPYAGKRRVTENIGKGHGMARVERIKTSPRFAAFVPFAVGGRRTHPPKVEKIIWEDINKKERRLAIMSAIAATANYDLVRARGHVVDNVPQVPIVVTDDLEKVFKTAQTREIFKKLGVWDDIERAKKNTKIRAGKGKMRGRRYKKAKGPLVVVAKNEGIVQGARNHPGVDVVTVENLSAELLAPGTHPGRLTIWTKGAIERLREIYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P724</accession>
    <name>A0A218P724_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerase subunit Rpo6</fullName>
            <ecNumber evidence="4">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">DNA-directed RNA polymerase subunit K</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpo6</name>
        <name evidence="4" type="synonym">rpoK</name>
        <name evidence="5" type="ORF">A3L08_04265</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the archaeal Rpo6/eukaryotic RPB6 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="4"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853851.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P724"/>
    <dbReference type="GeneID" id="33315457"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04265"/>
    <dbReference type="OrthoDB" id="10567at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00192">
        <property type="entry name" value="RNApol_arch_Rpo6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020708">
        <property type="entry name" value="DNA-dir_RNA_polK_14-18kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006110">
        <property type="entry name" value="Pol_omega/Rpo6/RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036161">
        <property type="entry name" value="RPB6/omega-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006111">
        <property type="entry name" value="Rpo6/Rpb6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002208">
        <property type="entry name" value="PRK01099.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47227">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47227:SF5">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01192">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000778">
        <property type="entry name" value="RpoK/RPB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63562">
        <property type="entry name" value="RPB6/omega subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01111">
        <property type="entry name" value="RNA_POL_K_14KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 4 5" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="2 4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="57" mass="6309" checksum="8B68C5CC29A81D9A" modified="2017-09-27" version="1">MFRYTRFEKARIIGARALQIAMGAPILIDVPEGITPLEAALLEFEKGIIPLTVIRPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P770</accession>
    <name>A0A218P770_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_04475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853885.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P770"/>
    <dbReference type="GeneID" id="33315499"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04475"/>
    <dbReference type="OrthoDB" id="97544at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="227" mass="25174" checksum="577C54605B7BDF7C" modified="2017-09-27" version="1">MGWKLPASVLMSAVISFFAWIVVQLNRPLGLATFALAFLLPVVTFRRYYSRKPRMLVPFGILVILIAILLTPPRATSLNLVSGLHLKTGCWDVEGSLWPLNESADVSQSCSYLVEPAGKVVHVSGMAWKNLEVRMPITSYPIVLRRPAEKADGAYLNAVQNARENGYAEAPGNFGPRESIIREVLMRKENHCVYIAEVRIAGGLAVVSVRGDCRAVDEFVQSRVRMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7B8</accession>
    <name>A0A218P7B8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_04730</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853932.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7B8"/>
    <dbReference type="GeneID" id="33315550"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04730"/>
    <dbReference type="OrthoDB" id="85965at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53795">
        <property type="entry name" value="PEP carboxykinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="34351" checksum="72577E0FB1600922" modified="2017-09-27" version="1">MRTGGVNIRFTGELDDGFRDAFFGLFARRYLPDVGVRSGDPQVIVERFKGDEFRVFSASYDHMGVDSYKIESGVPSAYGNEAPIFFILQAAARAGAKSGRMFITDSVGVVTPNGKAVLFVGYPHTGKSTMSVLALGHGLPVLSTENTVVEVRDGRLYIVGGTEVLVYDPRVEGIYDVEVPYDEQTRSGYRIKDMGMDDERKKLLRDGVEIDMIVLLHAAFNCMEASFSRIKGRKVRKTLWYFSTALMKGLDYYEPAPLHVPMNDEISRHLREFLETASSGYSDRMFEAFGNHRAVFERVLEMSSLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7C7</accession>
    <name>A0A218P7C7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl11</name>
        <name evidence="10" type="ORF">A3L08_04815</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the ribosomal stalk of the 50S ribosomal subunit. Interacts with L10 and the large rRNA to form the base of the stalk. L10 forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853943.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7C7"/>
    <dbReference type="GeneID" id="33315567"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04815"/>
    <dbReference type="OrthoDB" id="8842at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.250:FF:000006">
        <property type="entry name" value="50S ribosomal protein L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1550.10:FF:000007">
        <property type="entry name" value="50S ribosomal protein L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="entry name" value="Ribosomal protein L11/L12, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_uL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_uL11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020785">
        <property type="entry name" value="Ribosomal_uL11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_uL11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_uL11_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002232">
        <property type="entry name" value="PRK01143.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="Ribosomal L11/L12e N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00359">
        <property type="entry name" value="RIBOSOMAL_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL11 N-terminal" evidence="9">
        <location>
            <begin position="5"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL11 C-terminal" evidence="8">
        <location>
            <begin position="70"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="163" mass="17248" checksum="1C6412ED356E0662" modified="2017-09-27" version="1">MAQVVEVLVEGGKASPGPPLGPAIGPLGLNVKQVVDEINKATKDFEGMQVPVKIIVTDPKKKTFEIEVGVPPVSQLIKKEIGAPKGSSEPGHSPVGNLTMEQVIRIAKAKQEQMLAADLKAAAKEVIGTALSMGVTVEGKDPREVQKEIDEGVYDEIFANAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7D7</accession>
    <name>A0A218P7D7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glutamate--tRNA ligase</fullName>
            <ecNumber evidence="10">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Glutamyl-tRNA synthetase</fullName>
            <shortName evidence="10">GluRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 15" type="primary">gltX</name>
        <name evidence="15" type="ORF">A3L08_04870</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>tRNA(Glu) + L-glutamate + ATP = L-glutamyl-tRNA(Glu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="10"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853953.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7D7"/>
    <dbReference type="GeneID" id="33315578"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04870"/>
    <dbReference type="OrthoDB" id="10470at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043604">
        <property type="term" value="P:amide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09287">
        <property type="entry name" value="GluRS_non_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.10:FF:000033">
        <property type="entry name" value="Glutamate--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000222">
        <property type="entry name" value="Glutamate--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="entry name" value="Ribosomal Protein L25, Chain P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02076">
        <property type="entry name" value="Glu_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050132">
        <property type="entry name" value="Gln/Glu-tRNA_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004526">
        <property type="entry name" value="Glu-tRNA-synth_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_bL25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_bL25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049437">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00463">
        <property type="entry name" value="gltX_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003169">
        <property type="entry name" value="PRK04156.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097:SF5">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097">
        <property type="entry name" value="GLUTAMINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20974">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="Ribosomal protein L25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="12">
        <location>
            <begin position="99"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib anti-codon binding" evidence="13">
        <location>
            <begin position="409"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA synthetases class I (E and Q) anti-codon binding" evidence="14">
        <location>
            <begin position="509"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="10">
        <location>
            <begin position="106"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF20974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ASJ06697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="572" mass="66505" checksum="BD6AB8E5FE1C25D8" modified="2017-09-27" version="1">MEVETLVWKYALVNAYTHKGKANPKAVIGKVLGENPELRPKAKEIIPLVNEIVEKVNALSIEEQEAKLREIYPEFFEEKREKKEEKKGLPPLPKAEKGKVVTRFAPNPDGAFHLGNARAAILSHEYARMYDGKFILRFDDTDPKVKRPEPIFYEWINEDLEWLGFRVDEIHIASDRLEIYYDYAEKLIKMGKAYVCTCPPEKFRELRDKGIACPHRDEPVEVQLERWRKMLNGEYKEGEAVVRIKTDLKHPNPAVRDWPALRIIDNPDHPRVGDKYRVWPLYNFASAIDDHELGVTHIFRGQEHAENETRQRYVYDYLGWEYPVTVHHGRLSIEGVILSKSKTRKGIEEGKYLGWDDPRLGTIRALRRRGIQPEAIRELIIEVGLKRSDTTISWDNLAAINRRIVEPIANRYFFVADPIPMYIEGAEEFTAEIPLHPDHLERGVRRLKFEPGKPVYVSKDDMELFRPGNFVRLKDLFNVEVVEVSENGIKAKFHSVDYEIARENRWRMVHWVTEGKACEVLVPDGDELLVRKGLLEADAEVKVDEVVQFERFGFVRIDEVTPEKVVAIFAHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7F9</accession>
    <name>A0A218P7F9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_04985</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853972.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7F9"/>
    <dbReference type="GeneID" id="33315601"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04985"/>
    <dbReference type="OrthoDB" id="18264at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07391">
        <property type="entry name" value="MPP_PF1019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004376">
        <property type="entry name" value="Pesterase_MJ0037"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024173">
        <property type="entry name" value="Pesterase_MJ0037-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00024">
        <property type="entry name" value="SbcD_rel_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39323">
        <property type="entry name" value="BLR1149 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39323:SF1">
        <property type="entry name" value="BLR1149 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000887">
        <property type="entry name" value="Pesterase_MJ0037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="228" mass="25806" checksum="2128716C804C654C" modified="2017-09-27" version="1">MKPRPLPGKALRLGRTIVIADLHLGYEVSMAREGFYLPRVFHEVVESLRTILEKEKPKRLVIDGDLKHSFVPEWREREELKAFVEEVSPLVSELVLVRGNHDVGTIWLRELGVEVVDELEVGCWKLVHGHKLVEGERFIIGHEHPAIRLRDEVGALVKVPAFLFGEDLIVLPAFSPWAYGNDVLREIVSPFLRAYDVSRVRVLVPLDDELLDFGELGKLSRVLGSLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7G8</accession>
    <name>A0A218P7G8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Thiamine biosynthesis protein ThiS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853987.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7G8"/>
    <dbReference type="GeneID" id="33315624"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05100"/>
    <dbReference type="OrthoDB" id="104640at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd17506">
        <property type="entry name" value="Ubl_SAMP2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006231">
        <property type="entry name" value="PRK08364.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="67" mass="7330" checksum="9EA9405C80683C4D" modified="2017-09-27" version="1">MIRVKVLGRGIEKELEWEKGMKVADVLREIGFNTESAIAKVNGRVALEDESVKDGDYVEVIPVVSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7K7</accession>
    <name>A0A218P7K7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Iron-molybdenum cofactor-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854010.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7K7"/>
    <dbReference type="GeneID" id="33315649"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05225"/>
    <dbReference type="OrthoDB" id="25911at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00851">
        <property type="entry name" value="MTH1175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.130">
        <property type="entry name" value="Dinitrogenase iron-molybdenum cofactor biosynthesis domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003731">
        <property type="entry name" value="Di-Nase_FeMo-co_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036105">
        <property type="entry name" value="DiNase_FeMo-co_biosyn_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033913">
        <property type="entry name" value="MTH1175_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051840">
        <property type="entry name" value="NifX/NifY_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33937:SF2">
        <property type="entry name" value="DINITROGENASE IRON-MOLYBDENUM COFACTOR BIOSYNTHESIS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33937">
        <property type="entry name" value="IRON-MOLYBDENUM PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02579">
        <property type="entry name" value="Nitro_FeMo-Co"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53146">
        <property type="entry name" value="Nitrogenase accessory factor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dinitrogenase iron-molybdenum cofactor biosynthesis" evidence="1">
        <location>
            <begin position="16"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="109" mass="11809" checksum="44BF4C6706E23394" modified="2017-09-27" version="1">MVKTLVAVPTSKGGLDDEVHKSLVRAETFTLVEVEDGAVKDVKVIENPYRQEPYGAGSKVALFLVNLGINAIISRTDCPKGKMILDSAGIKMVVLDTPMKVGDVLRKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7L2</accession>
    <name>A0A218P7L2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">C4-dicarboxylate ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_05265</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tellurite-resistance/dicarboxylate transporter (TDT) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854018.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7L2"/>
    <dbReference type="GeneID" id="33315657"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05265"/>
    <dbReference type="OrthoDB" id="184482at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000319">
        <property type="term" value="F:sulfite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09321">
        <property type="entry name" value="TDT_like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.150">
        <property type="entry name" value="Voltage-dependent anion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004695">
        <property type="entry name" value="SLAC1/Mae1/Ssu1/TehA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051629">
        <property type="entry name" value="Sulfite_efflux_TDT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011552">
        <property type="entry name" value="TehA/Mae1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038665">
        <property type="entry name" value="Voltage-dep_anion_channel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00816">
        <property type="entry name" value="tdt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31686">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31686:SF1">
        <property type="entry name" value="SULFITE EFFLUX PUMP SSU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03595">
        <property type="entry name" value="SLAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="174"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="242"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="299"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="334" mass="37437" checksum="3F2F83A6AB8D6EED" modified="2017-09-27" version="1">MKVSVKDFAPSWFASVMGTGALALVSLAYSGRLAVLKNVAVWLTYFNTAMFFVLLIPWSLRWLKYRENSTKDLYHPMVSHFYGTIAIAMLVLSADYLLILRETAVAKAFWLIGTPLTIFFALLIPYLMFIQEKIDIKSVTPAWFIPPVGLIVIPLSGGPLIGTFHGIWKEIAILINYFAWGSGFFLYLALFAVVMHRFITHEPLPCGIAPAIWINLGPIGAGTSTLYMLVRNSDFITAKEPFLAFGIVFWGFGVWWLAMAVIMTLHYIRRLSLPYSLAWWAFIFPLGAYVSATHNVGTAFGIGAIDSFGFALYWLLLALWLLTGVKTLKHTLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7P6</accession>
    <name>A0A218P7P6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854045.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7P6"/>
    <dbReference type="GeneID" id="33315685"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05405"/>
    <dbReference type="OrthoDB" id="102256at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="318"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="349" mass="39241" checksum="4E08B3F45B23B486" modified="2017-09-27" version="1">MRGLKIFSLAFFSYLGLSLYANYFDEGLRDTIYSRGFELSMLVLGTVYALAFFAVFALGYSAKIELPPGFLAGLFFLLSLSRPVVITPVAFIVLVAYKKGVNPFRLFPEESLLVSLLIPLSLYVIVGIPLRDRCLRYELVGPLVLAAILAVIGIAYSKLSLRWKTLLLFVYSFLFFLGTFRSLTLLVFLAYFLTVYIGYPQLRKWLVPLSVFPLALVVGMSGGLEAVFIRVGFTFLVFHNLVRLSLPWGLFHGSLLLSNNPRHLVASLFDGPTNYTYFFFGQPIADFGLLGLFEAFLLGMFLRDSERTPESLVTVLSLMIYSIDPGVDAFLLMFIVSTLLFLRMERKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7S7</accession>
    <name>A0A218P7S7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Beta-ribofuranosylaminobenzene 5'-phosphate synthase</fullName>
            <shortName evidence="2">Beta-RFA-P synthase</shortName>
            <ecNumber evidence="2">2.4.2.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_05635</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the condensation of 4-aminobenzoate (pABA) with 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to produce beta-ribofuranosylaminobenzene 5'-phosphate (beta-RFA-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>5-phospho-alpha-D-ribose 1-diphosphate + 4-hydroxybenzoate + H(+) = 4-(beta-D-ribofuranosyl)phenol 5'-phosphate + CO2 + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:48556"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17879"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:82767"/>
            <dbReference type="EC" id="2.4.2.54"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta-RFA-P synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.54" evidence="2"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854087.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7S7"/>
    <dbReference type="GeneID" id="33315731"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05635"/>
    <dbReference type="OrthoDB" id="85156at2157"/>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043793">
        <property type="term" value="F:beta-ribofuranosylaminobenzene 5'-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004422">
        <property type="entry name" value="RFAP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00144">
        <property type="entry name" value="beta_RFAP_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF6">
        <property type="entry name" value="BETA-RIBOFURANOSYLPHENOL 5'-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861">
        <property type="entry name" value="HOMOSERINE/4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004884">
        <property type="entry name" value="Sugar_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="3">
        <location>
            <begin position="57"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="4">
        <location>
            <begin position="203"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR004884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="323" mass="35387" checksum="CC03BFB3E71DC13E" modified="2017-09-27" version="1">MIIRTPRRLHMGLIDPAAVFGRRFGSLGVALEGGYEVRVTEGERMEIVAEGEDRETIEAVLKKMNSAYETGVNYRVEVRKAIPRHVGLGSTTQLSLAVAVGIARLSGLKIPVEELAKTLGRGKNSGAGIYAFAHGGFVIDGGVREDIPPLIFREDFPEDWAFLLVIPGLRPGFDEEEEKPVMAGVVGRADVAMEISHRILLGLLPALNERDIRTFGEHLSAIQRLVGKHFEAYQGGEFREDVKLILGFLAEKTYGVGQSSWGPTVYGLIRKGEFQRLSAETHDFLKDHGLKASVELGIPKNSGADVIEESAFIERLIRNVAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7U4</accession>
    <name>A0A218P7U4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ABC transporter permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05645</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7U4"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05645"/>
    <dbReference type="OrthoDB" id="86287at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43471">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43471:SF12">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12679">
        <property type="entry name" value="ABC2_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="289" mass="31842" checksum="57D3A528F8FFA03F" modified="2017-09-27" version="1">MFWGFRLEFKQSLRTKKLWVILGVMMLLYIPGFYLQKSSGQEIETVGQAVSTLINGINGLGSFFIGILALLIGATAINSEIEKGTLRVAMSKPIKRLSYIGGKFLAHTAVLLLALLLTTVIGIIGVVWLGAPVKGQLITDSLLLNGLLLLAMVQLVALGYILSTFIKSSNTALGVALVVMFVVFMIMPSVIQFMAAKDTLLSENPDWQAYQEKSKEYQTKYLFYVPTTQIDVIVSDATVIEGDIANPSIEYVGIGHAIKSNPTNLFNLAFLTLVYLAIAFYRFLRMDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7U6</accession>
    <name>A0A218P7U6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable ribosomal RNA small subunit methyltransferase A</fullName>
            <ecNumber evidence="7">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">16S rRNA dimethyladenosine transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">16S rRNA dimethylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rsmA</name>
        <name evidence="7" type="synonym">ksgA</name>
        <name evidence="10" type="ORF">A3L08_05735</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854106.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7U6"/>
    <dbReference type="GeneID" id="33315751"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05735"/>
    <dbReference type="OrthoDB" id="9883at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000179">
        <property type="term" value="F:rRNA (adenine-N6,N6-)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000023">
        <property type="entry name" value="Ribosomal RNA small subunit methyltransferase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.100">
        <property type="entry name" value="Ribosomal RNA adenine dimethylase-like, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00607">
        <property type="entry name" value="16SrRNA_methyltr_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023165">
        <property type="entry name" value="rRNA_Ade_diMease-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011530">
        <property type="entry name" value="rRNA_adenine_dimethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00755">
        <property type="entry name" value="ksgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727:SF7">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 7 8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 7 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA adenine methylase transferase N-terminal" evidence="9">
        <location>
            <begin position="30"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="280" mass="32129" checksum="3EB659307CCD1418" modified="2017-09-27" version="1">MRERLFYLISKYNLKPNRNLGQNFLIVPDIIERNVERAGLSEKDTVLEVGPGLGVLTDALAGRAGKVYAIEKDSLLVKILRNEYDWPNVEIIEGDALKVPFPEFNKIVSNLPYQISSPVTFRFLKHEFERAVLIYQLEFAQRMVAKPGEKNYSRLSLMIRAKAHAEIAERIGRGAFWPKPEVDSAVVILEPKPPEERIELDENLVRALFQHRRSTVLSALKKSHHMLGLTKEEFKRVKGILSRIPHANERVFQLSPEDVWEIEEFLGEEGIIPLTPRVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7W7</accession>
    <name>A0A218P7W7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein eL21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl21e</name>
        <name evidence="7" type="ORF">A3L08_05720</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL21 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854103.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7W7"/>
    <dbReference type="GeneID" id="33315748"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05720"/>
    <dbReference type="OrthoDB" id="6295at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.70:FF:000001">
        <property type="entry name" value="60S ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.70">
        <property type="entry name" value="Ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00369">
        <property type="entry name" value="Ribosomal_eL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001147">
        <property type="entry name" value="Ribosomal_eL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022856">
        <property type="entry name" value="Ribosomal_eL21_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018259">
        <property type="entry name" value="Ribosomal_eL21_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036948">
        <property type="entry name" value="Ribosomal_eL21_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003303">
        <property type="entry name" value="PRK04306.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20981">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01157">
        <property type="entry name" value="Ribosomal_L21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01171">
        <property type="entry name" value="RIBOSOMAL_L21E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="98" mass="11226" checksum="F4DC79C919C46435" modified="2017-09-27" version="1">MVKKAHSFRRKTRGKLSKSPRRRGLPPLTRFLQEFEVGQKVHIVIEPSYHRGMPDPRFHGRTGTVVGKRGDAYVVQIKDGGKVKTFFIHPVHLRAQKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P829</accession>
    <name>A0A218P829_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Na+/H+ antiporter subunit G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854194.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P829"/>
    <dbReference type="GeneID" id="33315847"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06220"/>
    <dbReference type="OrthoDB" id="19138at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005133">
        <property type="entry name" value="PhaG_MnhG_YufB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01300">
        <property type="entry name" value="CPA3_mnhG_phaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009314">
        <property type="entry name" value="PRK12674.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34703">
        <property type="entry name" value="ANTIPORTER SUBUNIT MNHG2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34703:SF1">
        <property type="entry name" value="ANTIPORTER SUBUNIT MNHG2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03334">
        <property type="entry name" value="PhaG_MnhG_YufB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="135" mass="14657" checksum="F33EB42B53673DA4" modified="2017-09-27" version="1">MNALTAIGEVLVLIGTFFYLLSALGLIRMPDVYNRMQTSTKSATLGSLGVMIGVGIWALGEDFGSVAWLTKTIVIALFLLLTNPVSAHALIRAAYKSGIPLWEGSVMDKYREYIESKTRAPESEEGETPEEGGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P834</accession>
    <name>A0A218P834_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NADH dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_06200</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854190.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P834"/>
    <dbReference type="GeneID" id="33315843"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06200"/>
    <dbReference type="OrthoDB" id="371891at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050586">
        <property type="entry name" value="CPA3_Na-H_Antiporter_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006241">
        <property type="entry name" value="PRK08377.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42703:SF1">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER SUBUNIT D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42703">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01434">
        <property type="entry name" value="NADHDHGNASE5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="302"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="370"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="397"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="7">
        <location>
            <begin position="129"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="495" mass="52450" checksum="468F56F1E1742850" modified="2017-09-27" version="1">MNGQYASLLIALPLISAFFVPLIKGFGKKAIKYYLVLITAIQTGIAGWVFSEVYSTGKPIIVIAGGWRPPVGINLYLGHFAALFVFIVALVSFFMAVFSVKAIDVEPIDKYAMLFLLLMLGATGMIATGDLFNLFVFMEITAISAYALTAYNKTGEAAEASLKYIVLGGIGSSFFLVGVALLYGSLGTLNLAHIAQLAGGVNATVAQVSLALIIFGLAVEAELFPLNAWAPDAYQAAPHPITAMFSAFVVKAGLYAMARILYVLSAASGWANIARLLVIMGTLTVIVGELSALRQRDVKRMIAYSSIAQVGLIAVALALGTADGVSAGVFHMVNHAIVKALLFLAVGYVGITLGGTAVENFEGLGKRMPLTAFSITVGAVAVMGIPLFNVFWSKMRIILAAMGAGYAWVVALVLAASVVEAVYYIRLIHTMWFTGEGEKLNENLVFGVIMLLLVVAIIVIGIYPEPLWDIVQKAGNDIVNVAGYIKNVPLMGVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P850</accession>
    <name>A0A218P850_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854201.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P850"/>
    <dbReference type="GeneID" id="33315854"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06255"/>
    <dbReference type="OrthoDB" id="94027at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="235" mass="26661" checksum="5886F1D23F994469" modified="2017-09-27" version="1">MRGSAKALVILIGAVYLIVGYFLLSTSVSWESKGEFNALSNVSYSGRVFVDIPLELELMYDVYPELGHDEFKSFLRNAIEEVLISHNLWPDFTHVRPVVVLPGDSPPDRVNAPVVVIFTNFQGRENRFYYESCYASVLIYMNSNGDVGSYLSVERKYSEDSSKTDDLSNFARDLYETARARGNSTGDYSLKVVYWNVLEVRTGKFSGKNCWEMLTGEIVKEVDEWASTLDYSDEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P852</accession>
    <name>A0A218P852_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Class III signal peptide-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854216.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P852"/>
    <dbReference type="GeneID" id="33315870"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06335"/>
    <dbReference type="OrthoDB" id="382356at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007166">
        <property type="entry name" value="Class3_signal_pept_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04021">
        <property type="entry name" value="Class_IIIsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="66" mass="7557" checksum="F9617CDC7FECA59A" modified="2017-09-27" version="1">MGRLRAQGAVEYLLIMTLVLIILAWSIYNLRVAKRQHENLADFLSSSEDRFNEKIRSEISSSLDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P857</accession>
    <name>A0A218P857_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Prenyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06315</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P857"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06315"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="182" mass="20732" checksum="B1D9ECF5F33B3DB2" modified="2017-09-27" version="1">MFVLFGLSSNVALVGVLYLLLNIMYSYFLKRLFLIDVIAISIGYLLRTIAGAQVIDVPVSNWLVFSVFQVALILAFHKRRCELNVPSSIKHRNTLGEYTPGLIINLSTMTTASLVVTYLIYINEVNPKLVFTIPLVFYGVFRLEYLLIKCETDFEPQYLRDMPLIATLISWVVLVVISLYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P892</accession>
    <name>A0A218P892_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06245</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854199.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P892"/>
    <dbReference type="GeneID" id="33315852"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06245"/>
    <dbReference type="OrthoDB" id="381198at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="228" mass="24929" checksum="C6B630E604EFA81C" modified="2017-09-27" version="1">MRRKLLLVAGVVISLLLVVSLVIASENSVLAEYSGTTAHSSYPVVYESNESISLATPPKSSTVYIAVQADEPLKSLLENRLSERVRERGLRPIVVEVGDLPKVQLKGRVLFVYVSSLNEKDGFLVRIVTVDAIAYYSTAGDIVDFLSALQKCSKGVSSSDTERLMEIVSHETEANMDTKRIPGDAVFMYWEGLKAKSGILANSDPYMMLADYIADEIISGMRSVGDSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8A4</accession>
    <name>A0A218P8A4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutamate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06585</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854262.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8A4"/>
    <dbReference type="GeneID" id="33315920"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06585"/>
    <dbReference type="OrthoDB" id="27922at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009409">
        <property type="entry name" value="PRK12770.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42783">
        <property type="entry name" value="GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42783:SF3">
        <property type="entry name" value="GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="19"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="352" mass="38551" checksum="5BA43D831B8F7940" modified="2017-09-27" version="1">MSGMRFAFLCKSRPEPTGKKVAIVGAGPAGLSAAGYLVCQGHEVHVYDKLPEPGGLMLFGIPEFRIPIYRVREGCGELKDLFEVKFFPRTKVVCGECESEAGDEFVERTVHLAELKENYDAVLIATGAWEPWKPEVEGSELQGVYPALEYLFRIKSAKLGHMKWTDIIHPEGKKILVVGAGHTAVDAALESLLLGADEVYLSYRRSIKEAPAGPYEIHQLIQRGVKWLEKTMPVRIIGDGKVKAVEMVRMELGEPDASGRRRPVPVEGSEFTLEVDYVIFAVGQTPTPPAGDGLEIARDKKGRVVVDERHMTSVEGIFAAGDVVTGPSKVGLAVKDGLYAARSMHMWMMGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8F4</accession>
    <name>A0A218P8F4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Dipeptide/oligopeptide/nickel ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_06945</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8F4"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06945"/>
    <dbReference type="OrthoDB" id="18209at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43067:SF3">
        <property type="entry name" value="MALTOSE ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43067">
        <property type="entry name" value="OLIGOPEPTIDE/DIPEPTIDE ABC TRANSPORTER, ATPASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="338" mass="37851" checksum="9E6B549972C6DEFF" modified="2017-09-27" version="1">MNKRMVLENLRAYYRQLKGTTEYLVKAVDGVNLEIYEGDILGLVGESGCGKSTLAKTMMMDLTPPLQYMGGKLQIISRDGTVFDITQFKSKDQVKRTLWGKHIAYVPQDALNALMPTIKIKKIAYDVLRSNESGITFQEAVKKAKERLTELDLPDYVTELYPFQLSGGMRQRAVLAMATLLNPEILIVDEPTSALDVTTQKIVLKSLLKLRKLELVDSIVFITHDISTVRQIANRIAVMYAGKIVEAGPTESIIKEPLHPYTKALIEAVASIEPEGREKGLSYIPGQPPNLINPPTGCRFYPRCPYAMEVCRKEEPELVKIGETQVACWLYKGGRENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8L6</accession>
    <name>A0A218P8L6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_07250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854383.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8L6"/>
    <dbReference type="GeneID" id="33316053"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07250"/>
    <dbReference type="OrthoDB" id="85694at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="86" mass="9516" checksum="B6B9DE875D24C7D8" modified="2017-09-27" version="1">MLEKVLEMLKTGKDVDEIAEELNVPREEVIGAMEVLASMGYIERVDAGEGACATCPLRNFCPGSCFRFKGKVYTVAEVKLEKSSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8M8</accession>
    <name>A0A218P8M8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_07310</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC-3 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854394.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8M8"/>
    <dbReference type="GeneID" id="33316065"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07310"/>
    <dbReference type="OrthoDB" id="11310at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="entry name" value="ABC transporter involved in vitamin B12 uptake, BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001626">
        <property type="entry name" value="ABC_TroCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30477:SF21">
        <property type="entry name" value="ABC-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30477">
        <property type="entry name" value="ABC-TRANSPORTER METAL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00950">
        <property type="entry name" value="ABC-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="ABC transporter involved in vitamin B12 uptake, BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="205"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="273" mass="29112" checksum="A0ECB175860D515F" modified="2017-09-27" version="1">MIPEYIIRALLASIMVSVLLGMLSPLINTKGLAFLTHALFHALLFGAVLGMILGLLFENLSLVMLTALFVTVVIVLLIAHLEKLGFSPDSAVGIVASFVAGLTVLGFGVLYKVMASRPYFPLGESIVSYLTGDIFLISLNDLTALVLGGAALFFVMLFLYRDFLYLSFDPEGMESYGGNVRAYLMVLYVLVGAIGALIVQTVGLITLQVVAVLPGAIALMVSSDLRKVIGVSLFLTLGVQLSSVVLAYLTDIPPSGLATIMLGAIYGVLLFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8R4</accession>
    <name>A0A218P8R4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854391.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8R4"/>
    <dbReference type="GeneID" id="33316062"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07295"/>
    <dbReference type="OrthoDB" id="56872at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071164">
        <property type="term" value="F:RNA cap trimethylguanosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741">
        <property type="entry name" value="S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741:SF32">
        <property type="entry name" value="TRIMETHYLGUANOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="1">
        <location>
            <begin position="87"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="383" mass="44503" checksum="6D27D629F21E847A" modified="2017-09-27" version="1">MLEKISEEKVREAVELIRRGLDERKLRARLGEDWELIAEIARARIKARDKFSRDDLWMDLQGLRYSTHEEVAKYRARRLKEFGVKSIADVSCGIGIQLIFYAMNVERAYGIDIDPLKVEFARRNAEKYGVSNIEFINADSLSPEIAEKIDAEVIFSDPARPPEMPERRLEDLLPSPLEVYRAYGRKTDAFIFDLPPQIRRERVPWKGEFEYIDLFGALNRLTFYTEPLARAERSAVILPAGARVESDPNLENIVEWTDEPGEYLYEIPQSVDYADLINELLHLLPVEARMLLREKRRVLATGDEEIRSPYLKRTYRVVGIVPFHPVRINDFLRKEGFGRATLRISVPDGEYWRFRKRVEANLSGERRAFVFQLGDRAVIAEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8T1</accession>
    <name>A0A218P8T1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Thymidylate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_07490</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IMPACT family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854425.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8T1"/>
    <dbReference type="GeneID" id="33316101"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07490"/>
    <dbReference type="OrthoDB" id="121633at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.30">
        <property type="entry name" value="Impact, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023582">
        <property type="entry name" value="Impact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001498">
        <property type="entry name" value="Impact_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036956">
        <property type="entry name" value="Impact_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015796">
        <property type="entry name" value="Impact_YigZ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015269">
        <property type="entry name" value="UPF0029_Impact_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020569">
        <property type="entry name" value="UPF0029_Impact_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00257">
        <property type="entry name" value="IMPACT_YIGZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301:SF20">
        <property type="entry name" value="IMPACT FAMILY MEMBER YIGZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301">
        <property type="entry name" value="IMPACT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09186">
        <property type="entry name" value="DUF1949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01205">
        <property type="entry name" value="Impact_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00910">
        <property type="entry name" value="UPF0029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Impact N-terminal" evidence="2">
        <location>
            <begin position="17"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="UPF0029" evidence="3">
        <location>
            <begin position="139"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="205" mass="22539" checksum="2A864A1AF9FFC57A" modified="2017-09-27" version="1">MGYRTLKGPGTAQFIVKKSVFIGYASPANTEDEAKAFIAKIKAHHSDATHNVSAYLINDGKNFAVRYDDDGEPKGSAGKPVLKVIQNKGLSNVVVVVTRYFGGIKLGYGGLVKAYSDAASLAIENAGIVEVYETERFEVTFPYNLFHTVRETVESAGGKVVDEEYGELVKFTVETRKGEAEPLMELLTEKTRGRARLRPLFMRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8X3</accession>
    <name>A0A218P8X3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MBL fold metallo-hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07775</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854472.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8X3"/>
    <dbReference type="GeneID" id="33316157"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07775"/>
    <dbReference type="OrthoDB" id="7773at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07713">
        <property type="entry name" value="DHPS-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041712">
        <property type="entry name" value="DHPS-like_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052926">
        <property type="entry name" value="Metallo-beta-lactamase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13754:SF18">
        <property type="entry name" value="7,8-DIHYDROPTERIN-6-METHYL-4-(BETA-D-RIBOFURANOSYL)-AMINOBENZENE-5'-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13754">
        <property type="entry name" value="METALLO-BETA-LACTAMASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="21"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="276" mass="30977" checksum="EA82EC2DD94002AC" modified="2017-09-27" version="1">MRVTVLVEDYSGYESPFLAQHGVSFLVEKGGKRILFDTGQSAEPVLHNMELLGIEPGSIDYIFLSHCHYDHTGGLLGILKAVGRRIPVIAHPEIFRKHFITKPYLRSVGIPFRRDEIEELAELYFTAELIEIVEDLYSTGEIRGREDFEKANLEVYTLRGRKVIKDELLDDTSLVAKTEEGLVVVSGCSHAGIVSIVKHAIRLTGEKRVKAIIGGFHLIDASEERIERTVREFLELGVEEVYTGHCTGLKAEAAFLQAYGERFHKLHSGVVIDFPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P920</accession>
    <name>A0A218P920_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">thioredoxin-dependent peroxiredoxin</fullName>
            <ecNumber evidence="2">1.11.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Thioredoxin peroxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">A3L08_07810</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peroxiredoxin family. BCP/PrxQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="2"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854478.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P920"/>
    <dbReference type="GeneID" id="33316165"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07810"/>
    <dbReference type="OrthoDB" id="145578at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03017">
        <property type="entry name" value="PRX_BCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000007">
        <property type="entry name" value="Thioredoxin-dependent thiol peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050924">
        <property type="entry name" value="Peroxiredoxin_BCP/PrxQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42801:SF4">
        <property type="entry name" value="AHPC_TSA FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42801">
        <property type="entry name" value="THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="11">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ07230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="153" mass="17372" checksum="D443F0D38AF0184C" modified="2017-09-27" version="1">MNPLEVKVLDEEGKELPLKDAVLGRWTVLYVYPKDNTPGCTTEAKEFTELLPEFEKLGFQVIGVSKDSVKSHLKFKEKHNLKVRLLSDPNAELIKALGAWGKKKRYGKEYEGVIRSTFILNPEGEIVWKKINVRAKGHAAKVLEEAKKLVRAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P959</accession>
    <name>A0A218P959_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">4-oxalocrotonate tautomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_08325</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854572.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P959"/>
    <dbReference type="GeneID" id="33316271"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08325"/>
    <dbReference type="OrthoDB" id="358896at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.429.10">
        <property type="entry name" value="Macrophage Migration Inhibitory Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004370">
        <property type="entry name" value="4-OT-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014347">
        <property type="entry name" value="Tautomerase/MIF_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041920">
        <property type="entry name" value="DmpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01361">
        <property type="entry name" value="Tautomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55331">
        <property type="entry name" value="Tautomerase/MIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4-oxalocrotonate tautomerase-like" evidence="2">
        <location>
            <begin position="2"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="65" mass="7418" checksum="E0A78F706D15B226" modified="2017-09-27" version="1">MPTIIVEGPKADKETKRELVKRLTEVVREVYGVHHVSVIIHENETENVGVDGELLSDILERRRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P962</accession>
    <name>A0A218P962_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Mechanosensitive ion channel protein MscS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_08320</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854571.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P962"/>
    <dbReference type="GeneID" id="33316270"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08320"/>
    <dbReference type="OrthoDB" id="31543at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049278">
        <property type="entry name" value="MS_channel_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008910">
        <property type="entry name" value="MSC_TM_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045275">
        <property type="entry name" value="MscS_archaea/bacteria_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011066">
        <property type="entry name" value="MscS_channel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221:SF19">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05552">
        <property type="entry name" value="MS_channel_1st_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21082">
        <property type="entry name" value="MS_channel_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82689">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="8">
        <location>
            <begin position="101"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS C-terminal" evidence="9">
        <location>
            <begin position="174"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ07324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="270" mass="29391" checksum="A5055A5FFFBDD158" modified="2017-09-27" version="1">MVGFDEPLPYVGITPLQIITAVVVLVVGWIVAKIVVTSFKRGLKKTKLPDLVVEFLGRFLSALLYVAVILLAVRALGIEVGSVVMGLSAVIGLILGFGMQDTLTNLAAGVWIAALRPIDMGEVVEVAGKTGKVNGVGIMSTELLTPDNVLITIPNKLVWGNVITNYTRMPTRRVDVDVGVAYGTDLDRAIKLAMDLMKNHPKVLKDPEPSVVITALADSSINLQLRAWVKTEDYWAVKGDLTKGIYELYTKEGIEIPFPQMDVHIKEMPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P966</accession>
    <name>A0A218P966_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mercury-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_08080</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P966"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08080"/>
    <dbReference type="OrthoDB" id="44171at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000001">
        <property type="entry name" value="ATPase copper transporting beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="2">
        <location>
            <begin position="2"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="68" mass="7537" checksum="E58BE916E9E03A5B" modified="2017-09-27" version="1">MTKAVLKIPNMSCKHCVMRIEKAIKSAGAEGKADLETKTAVVEFDPGKVRLEEIIKAIERYGYEVKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P971</accession>
    <name>A0A218P971_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P971"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08280"/>
    <dbReference type="OrthoDB" id="102364at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="247" mass="27830" checksum="A0D73EB3F032FE56" modified="2017-09-27" version="1">MVGVERVKVAVPLIALMLILMGLGNVSGAQMSGNVLHVWSYDMKSHYVEIHKGNKQLGNGDVFAVEQTVFVTNYSGSAYVALLEVQGTSNNNNDNTYSIYWYGENEVLLKTPTNSTTIPLDMSKPHRYRIEMTTTSVRFVIDSTTTVEFPANVKKVQRVNSGAWDDNCTYDMYIDDVKEYWNGQLVASEDFEKDEDGDDNNDDYYKDDHTISRNAYEDSGETVTDSTQVPEFPFLKPLFDYLSSVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9A0</accession>
    <name>A0A218P9A0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_08415</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854590.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9A0"/>
    <dbReference type="GeneID" id="33316289"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08415"/>
    <dbReference type="OrthoDB" id="384825at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="185" mass="20989" checksum="0910997455EB39E7" modified="2017-09-27" version="1">MALHSRPDGFVYDDDTGKGIIFTGSKRGSTIAVNFVAYLKMSAVENARPLYAAVGIEKLREEPSEFWTEDLLTDVIRGRGIKVHSLEIHNGKEIITATLKLEMGVIAHGRPSSYDVKELSDAVLGLIMDIVDEIIYWDHYVPEEDDFGDGLTWEFTDSWKSEDQSVDIFGSIEFWWEQFHGEEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9B9</accession>
    <name>A0A218P9B9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MBL fold metallo-hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_08655</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854629.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9B9"/>
    <dbReference type="GeneID" id="33316337"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08655"/>
    <dbReference type="OrthoDB" id="40950at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43694">
        <property type="entry name" value="RIBONUCLEASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43694:SF1">
        <property type="entry name" value="RIBONUCLEASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="88"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Zn-dependent metallo-hydrolase RNA specificity" evidence="2">
        <location>
            <begin position="462"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="517" mass="59174" checksum="2188EF58530EFDE5" modified="2017-09-27" version="1">MKITVYDGATTIGGSKIHLEEDNNGIFLDFGMNFAKYARYYEEFISERPSRGIHDLWRLGLIPRLNVYRRDLIPADILEEVRQSPKVSVSAVLISHAHLDHVGNIAFLDEKVPIVGSPTTMIILKALRDTSRGSHMGMELSYYALRKPSDSNPYVLEANRKLKYYASRDIFLTEKLPEEGMSFLTWRANVELAENPNRVNYIRGSNVSLVEDQDLGFEIRAYPVDHSIFGAIAYIVEGDISVAYTGDFRLHGKNADKTKEFIRAAKSAQVLITEGTRAGRGDDVNVSEEEVYQNAKAIVDDAKGLVVADFSARNFERLESFKRIAEETGRQLVVTTKDAYFLHALGLIDGQNHLKGLRVYRNSKAKVEKWEEWVFINYPEIGITPEELRREQENYILCFSFYDMPHLLDVMPDGGVYIYSSSEAFTEEQTFSFLRLWNWLQYFRFEVHGFSVDADGKPTFEKGLHASGHISKEELEEVINDIDPDVLIPVHTESPYWFEERWSEKVVLLKDGESFEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9E2</accession>
    <name>A0A218P9E2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Alanine glyoxylate aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_08775</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854650.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9E2"/>
    <dbReference type="GeneID" id="33316361"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08775"/>
    <dbReference type="OrthoDB" id="372018at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901605">
        <property type="term" value="P:alpha-amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000053">
        <property type="entry name" value="Aminotransferase, class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050859">
        <property type="entry name" value="Class-I_PLP-dep_aminotransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790:SF19">
        <property type="entry name" value="KYNURENINE_ALPHA-AMINOADIPATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="7">
        <location>
            <begin position="35"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="404" mass="45020" checksum="F3724B5836296140" modified="2017-09-27" version="1">MRYEKFLAGRANWVKGSALAEVMKKAAELRARGKELISLSAGDPDPNLIPREILGELAREVLSNVPASVMYTPANGIPELREEISRFLERYEGYKVSPEDIIITVGGTGALDLLGRILIDPGDAVITENPSYINTLLAFEQLGAKIYGVPVDGEGMRVDLLEEKLRELRAKGVKVKFIYTIPTGQNPMGVTMSHERRKALLEIASEHDLLIVEDSAYNFMRYEGEATPLKAMDDEGRVIVAGTLSKVLGTGFRVGWVIAHGEVKQKILMEKSPIDFCAPTISQYIALEYLRRGYFEEYHLKRGLLGYKEKRDVMLSALEENLPDAEFTRPIAGMFVMFFLPSGADGMAFASELMEKKGVVVVPGGPFYTDESGKNAIRLNFSRPGKEEIEEGIKRLAELYREKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9I0</accession>
    <name>A0A218P9I0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA double-strand break repair protein Mre11</fullName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">mre11</name>
        <name evidence="12" type="ORF">A3L08_08750</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. The complex may facilitate opening of the processed DNA ends to aid in the recruitment of HerA and NurA. Mre11 binds to DSB ends and has both double-stranded 3'-5' exonuclease activity and single-stranded endonuclease activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="9">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Nuclease activity is regulated by Rad50.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer. Forms a heterotetramer composed of two Mre11 subunits and two Rad50 subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the MRE11/RAD32 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854646.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9I0"/>
    <dbReference type="GeneID" id="33316356"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08750"/>
    <dbReference type="OrthoDB" id="11638at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045027">
        <property type="term" value="F:DNA end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000403">
        <property type="term" value="F:Y-form DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.80">
        <property type="entry name" value="DNA double-strand break repair nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02044">
        <property type="entry name" value="Mre11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050535">
        <property type="entry name" value="DNA_Repair-Maintenance_Comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032885">
        <property type="entry name" value="Mre11_archaea-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30337">
        <property type="entry name" value="COMPONENT OF ATP-DEPENDENT DSDNA EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30337:SF0">
        <property type="entry name" value="NUCLEASE SBCCD SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 9" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="406"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="412"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ07400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="476" mass="54441" checksum="8CA9F6FD90352F24" modified="2017-09-27" version="1">MKFAHIADVHLGREQFNQPFRYDDYLKAFRESIEKAVKADVDFILIAGDLFHVSRPSPRTIRDAVEVLELPRRKGIPVFAIEGNHDKTIRETSVFDLLEHLGLIHTVGLKREPRKGEFQESKKLSENRYLVWGKVGDLEIHGLRHHTRWQLIRNDGAINVLKALFKGKKGILMLHQAIDYLAKDTPYQDAFDLRLSELPDGFSYYALGHIHVRRIAEPSQTGLSGPLAYPGSLERTEVREASHIIRYSTRDKKPKVIENREGPKGFYIVEDFVPEFVGVDARPFYSVRVKGESKSELRRKVEEVANLIPKDAIAVITLEGTVKGGIALAEFNDLLKDSGVRYYTFRSRVVGEAVLSKERLSEEELFTEWERELLLHLRVEPKEFSEGLTEFVSWLMERYEKGIPQKAMVPKKTPEKKAEPGEKAPKKAKVPEKAPKEKKPSETKKPEKEEKKPQNKPVSKPKNPSSLDAWLGRGKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9I9</accession>
    <name>A0A218P9I9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13 16">Nucleoside diphosphate kinase</fullName>
            <shortName evidence="13">NDK</shortName>
            <shortName evidence="13">NDP kinase</shortName>
            <ecNumber evidence="3 13 16">2.7.4.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Nucleoside-2-P kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ndk</name>
        <name evidence="18" type="ORF">A3L08_08800</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:18113"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13 14 15">Belongs to the NDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="3 13 16"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854655.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9I9"/>
    <dbReference type="GeneID" id="33316366"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08800"/>
    <dbReference type="OrthoDB" id="6874at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NDK-like_dom"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PRK00668.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11349">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="11 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 13" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="8 13 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 13" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside diphosphate kinase-like" evidence="17">
        <location>
            <begin position="6"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="13 14">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <feature type="binding site" evidence="13 14">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <feature type="binding site" evidence="13 14">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022840"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ASJ07410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="174" mass="19978" checksum="C67C7E3B0DD88BD3" modified="2017-09-27" version="1">MADKQIERTLVILKPDAVVRGLMGEIISRFEKRGLKIVGMKMMWIDRELAEKHYEEHKGKPFFEPLIDYITKAPSVVMVVEGRYAISVVRKMAGATDPKDAEPGSIRGDYGLDVGDAIYNVIHASDSPESAEREINLYFKPEELFEYCKAADWFYHTHARTKKEYLDSTDCLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9J1</accession>
    <name>A0A218P9J1_9EURY</name>
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        <submittedName>
            <fullName evidence="4">Methylmalonyl-CoA epimerase</fullName>
        </submittedName>
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        <name evidence="4" type="ORF">A3L08_09045</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
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            <taxon>Archaea</taxon>
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        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methylmalonyl-CoA epimerase family.</text>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03081">
        <property type="entry name" value="metmalonyl_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43048">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR43048:SF3">
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    </dbReference>
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        <property type="entry name" value="Glyoxalase_4"/>
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        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="3">
        <location>
            <begin position="4"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="132" mass="14667" checksum="74066C577F883AD2" modified="2017-09-27" version="1">MIKKIDHVGIAVKNLDEAIKVWEGLGLRVEEIEEVPDQKVRTAIIHVGESRIELLEPTAEDSPIAKFIAKRGEGIHHIALGVDDIEKHLEELKEKGYRLIDEKPRIGAGGAKIAFVHPKAVTGVLLELCQRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9L6</accession>
    <name>A0A218P9L6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF63 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854707.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9L6"/>
    <dbReference type="GeneID" id="33316425"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09095"/>
    <dbReference type="OrthoDB" id="84937at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002749">
        <property type="entry name" value="DUF63"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40700:SF1">
        <property type="entry name" value="DUF63 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40700">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED, DUF63 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01889">
        <property type="entry name" value="DUF63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="271" mass="31108" checksum="74F3B7381A56EA2E" modified="2017-09-27" version="1">MGLYEFFYKYFVLPIKENQGYNPVNTVTYAIILGIAVILLYKMLKRMGIRVDDRFFKALIPYIILGPLMRGMTDVGILPRTYLTVSPGGYFVIAAFAIASLYVVWKHIGPGEKLYPLYRDFGWILVGGLLFVLVINLDRVSFNWKALGYFVPFLIIAEAFIWLIAKKAPLIADNKTLFYTHFYDATTTFVGIQFFGFWEQHVLARWLMDTLGTPAVMYLEKFFILLPVVWILDREMKDEDPDLINFVKLTVFILGFGPGTRNLLIMLMGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9M7</accession>
    <name>A0A218P9M7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NAD(P)H-flavin oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_09275</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854737.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9M7"/>
    <dbReference type="GeneID" id="33316461"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09275"/>
    <dbReference type="OrthoDB" id="287850at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02062">
        <property type="entry name" value="Nitro_FMN_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="entry name" value="NADH Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050627">
        <property type="entry name" value="Nitroreductase/BluB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23026:SF90">
        <property type="entry name" value="IODOTYROSINE DEIODINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23026">
        <property type="entry name" value="NADPH NITROREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="FMN-dependent nitroreductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="4">
        <location>
            <begin position="7"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="202" mass="23421" checksum="97DFA9EC87C6DD40" modified="2017-09-27" version="1">MELDEAILRRTSVRYFSDEPVSDDAIRELVKAAIRAPTASGLENWLFVAFRSEEARKRAFDLIAEGMVEYYRAVNLPEEKIEKLKNRIYEEGMYRAPVYIAVFIDRRVRFLKGKEFDEVEFIWSVESAAMAIQNLMLKAVELGLGTVYIGVTNFRGIEEKVRELAGLDDNYYLVGLIPVGYPREEVKPRKRRKGIEEVLKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9N4</accession>
    <name>A0A218P9N4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_09020</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ABC transporter superfamily. Drug exporter-1 (DrugE1) (TC 3.A.1.105) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854694.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9N4"/>
    <dbReference type="GeneID" id="33316410"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09020"/>
    <dbReference type="OrthoDB" id="31298at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043215">
        <property type="term" value="P:daunorubicin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900753">
        <property type="term" value="P:doxorubicin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03265">
        <property type="entry name" value="ABC_DrrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000589">
        <property type="entry name" value="ABC transporter, ATP-binding subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005894">
        <property type="entry name" value="DrrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025302">
        <property type="entry name" value="DrrA1/2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01188">
        <property type="entry name" value="drrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43582:SF4">
        <property type="entry name" value="ANTIBIOTIC RESISTANCE ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43582">
        <property type="entry name" value="LINEARMYCIN RESISTANCE ATP-BINDING PROTEIN LNRL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13732">
        <property type="entry name" value="DrrA1-3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="6"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ07450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="325" mass="37250" checksum="80BD29B980694F9B" modified="2017-09-27" version="1">MSEYAIEVENLVKKYGDFEAVKGISFKVRRGEIFAFLGPNGAGKTTTVHVLTTLLKPTSGKVFVAGHDVVAEPNEVRRKIGIVFQDPSLDRELTAYENMYLHGRIYGLGGNELREKIEKLLKFVELWDFRDRQVKTFSGGMRRRLELARSLLHEPEVLFLDEPTVGLDPQTRAHIWDYIRVMKEEHNMTIFLTTHYMDEAEMLADRIAIMDHGKIIAEGTAEELKKLIGNDVVYLKLEGPEEIRCLKADFIRGCKLLPDGRVALEVENAAEALPRLFELAKERGVKILEVTYHRPTLNDVFLHLTGREIRDEGGEQKFPMPFKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9N5</accession>
    <name>A0A218P9N5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">acetate--CoA ligase (ADP-forming)</fullName>
            <ecNumber evidence="3">6.2.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_09310</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP + CoA = acetyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15081"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the acetate CoA ligase beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.13" evidence="3"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854744.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9N5"/>
    <dbReference type="GeneID" id="33316468"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09310"/>
    <dbReference type="OrthoDB" id="18103at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043758">
        <property type="term" value="F:acetate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000020">
        <property type="entry name" value="Protein lysine acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051538">
        <property type="entry name" value="Acyl-CoA_Synth/Transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43334:SF1">
        <property type="entry name" value="3-HYDROXYPROPIONATE--COA LIGASE [ADP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43334">
        <property type="entry name" value="ACETATE--COA LIGASE [ADP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13549">
        <property type="entry name" value="ATP-grasp_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="9">
        <location>
            <begin position="26"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ07501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="242" mass="27295" checksum="68AA9117DBB7D767" modified="2017-09-27" version="1">MKEEALKVIESVLAQGRTALVEYEAKQVLKAYGLPVPEEKLAKTLDEALKYAEEIGYPVAMKLMSPQILHKSDAKVVLLNIKTPEELKEKWEVIHENARKYRPDAEILGVLIAPMLRPGREIIIGVTEDPQFGHAIMFGLGGIFVEVLKDVTFRIIPITERDARKMIQEIKSYPILAGARGEEPADIDAIVQLLLKVSELVDDLRDYIKEMDLNPVFVYERGKGAVVVDARIIVKEPKENRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9N8</accession>
    <name>A0A218P9N8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Endonuclease III</fullName>
            <ecNumber evidence="11">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">DNA-(apurinic or apyrimidinic site) lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">nth</name>
        <name evidence="13" type="ORF">A3L08_09335</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="11">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the Nth/MutY family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854749.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9N8"/>
    <dbReference type="GeneID" id="33316473"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09335"/>
    <dbReference type="OrthoDB" id="84708at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000703">
        <property type="term" value="F:oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1670.10:FF:000001">
        <property type="entry name" value="Endonuclease III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000031">
        <property type="entry name" value="Endonuclease III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00942">
        <property type="entry name" value="Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004036">
        <property type="entry name" value="Endonuclease-III-like_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003651">
        <property type="entry name" value="Endonuclease3_FeS-loop_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004035">
        <property type="entry name" value="Endouclease-III_FeS-bd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005759">
        <property type="entry name" value="Nth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43286">
        <property type="entry name" value="ENDONUCLEASE III-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43286:SF1">
        <property type="entry name" value="ENDONUCLEASE III-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10576">
        <property type="entry name" value="EndIII_4Fe-2S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001435">
        <property type="entry name" value="Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00525">
        <property type="entry name" value="FES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00764">
        <property type="entry name" value="ENDONUCLEASE_III_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01155">
        <property type="entry name" value="ENDONUCLEASE_III_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="10 11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 11" id="KW-0408">Iron</keyword>
    <keyword evidence="7 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="12">
        <location>
            <begin position="56"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ07505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="237" mass="27344" checksum="89D931513AD84D72" modified="2017-09-27" version="1">MVTASKSLDLSAFTFEESWEEKKERAKKIVEILMKTYPREKLLIGDPYRTLVHCIISQRMRDEVTYRVWEELFKRYKDIETIAATPVEEMQGFLRKQGVGLWKTKGEWIVKASRIILEKYGGKVPDDINELMKLPGIGRKCANIVLAYGFGKQAIPVDTHVNRISKRLGLAPPRVVPEKVEEYLTELIPRDKWIYVNHAMVDHGRSICRPIRPKCGECPLKELCPYARGLVGDEDIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9R8</accession>
    <name>A0A218P9R8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Small ribosomal subunit protein eS19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rps19e</name>
        <name evidence="7" type="ORF">A3L08_09475</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">May be involved in maturation of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 6">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic ribosomal protein eS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854775.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9R8"/>
    <dbReference type="GeneID" id="33316501"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09475"/>
    <dbReference type="OrthoDB" id="371836at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000449">
        <property type="entry name" value="30S ribosomal protein S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01474">
        <property type="entry name" value="Ribosomal_eS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001266">
        <property type="entry name" value="Ribosomal_eS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027548">
        <property type="entry name" value="Ribosomal_eS19_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018277">
        <property type="entry name" value="Ribosomal_eS19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006811">
        <property type="entry name" value="PRK09333.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01090">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01413">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00628">
        <property type="entry name" value="RIBOSOMAL_S19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6 7" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="150" mass="17307" checksum="A7C3C122F254156A" modified="2017-09-27" version="1">MATVYDVPGDLLVERVAQKLKEIEAIKPPEWAPFVKTGRHKERLPEQDDWWYYRVASVFRKVYVDGPVGIERLRTWYGGRKNRGHAPEHFYKASGSIIRKALQQLEAAGFVQKVPGEGRVVTPQGQSFLDKIATELKKELEEQIPELKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9S4</accession>
    <name>A0A218P9S4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Seryl-tRNA synthetase</fullName>
            <shortName evidence="8">SerRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Seryl-tRNA(Ser/Sec) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">serS</name>
        <name evidence="13" type="ORF">A3L08_09410</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>tRNA(Sec) + L-serine + ATP = L-seryl-tRNA(Sec) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42580"/>
            <dbReference type="Rhea" id="RHEA-COMP:9742"/>
            <dbReference type="Rhea" id="RHEA-COMP:10128"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>tRNA(Ser) + L-serine + ATP = L-seryl-tRNA(Ser) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12292"/>
            <dbReference type="Rhea" id="RHEA-COMP:9669"/>
            <dbReference type="Rhea" id="RHEA-COMP:9703"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. The tRNA molecule binds across the dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854762.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9S4"/>
    <dbReference type="GeneID" id="33316488"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09410"/>
    <dbReference type="OrthoDB" id="35932at2157"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016260">
        <property type="term" value="P:selenocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00770">
        <property type="entry name" value="SerRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000048">
        <property type="entry name" value="Serine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="entry name" value="Serine-tRNA synthetase, tRNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00176">
        <property type="entry name" value="Ser_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033729">
        <property type="entry name" value="SerRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="12">
        <location>
            <begin position="199"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="71"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="252"/>
            <end position="254"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <begin position="283"/>
            <end position="285"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="299"/>
            <end position="302"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 9">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <begin position="370"/>
            <end position="373"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="406"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for serine binding" evidence="9">
        <location>
            <position position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ07518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="455" mass="52899" checksum="4347B4FB72D6A9CE" modified="2017-09-27" version="1">MLDIKLIRENPDLVKGDLIKRGELEKLKWIDEILELDAKWRENLRKINQLRKERNRLAVQIGKRKKAGEPIDDLLAKSNEIVKQIEALEKEVEELRKKIDYYLWRLPNITHESVPVGKDDTENVPIRFWGKAKVWEGFLDTFKEQSLGKMEYEVLSWKPRLHVDMLELLRGADLERAAKVSGARFYYLMNELVILDLALIRFALDRLIEKGFVPVIPPYMVRRFVEEGVTSFGDFEDVIYKVEGEDLYLIPTAEHPLAGMHANEIIEGKDLPLLYVGVSPCFRKEAGTAGKDTKGIFRVHQFHKVEQFVYSRPEESWEWHEKLITNAEEIFQELEIPYRVVNICTGDLGYVAAKKYDIEAWMAGQGKFREVVSASNCTEWQARRLNIRFRDKTHEKPRFVHTLNSTAIATSRAIVAILENHQTEEGVVKLPKALWKYTGFKEILPASMKEKCCQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9V4</accession>
    <name>A0A218P9V4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Nicotinamide-nucleotide adenylyltransferase</fullName>
            <ecNumber evidence="4 5">2.7.7.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">NAD(+) diphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">NAD(+) pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">NMN adenylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_09690</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>beta-nicotinamide D-ribonucleotide + ATP + H(+) = diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21360"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="EC" id="2.7.7.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the archaeal NMN adenylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.1" evidence="4 5"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854815.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9V4"/>
    <dbReference type="GeneID" id="33316544"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09690"/>
    <dbReference type="OrthoDB" id="264480at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00600"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000309">
        <property type="term" value="F:nicotinamide-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02166">
        <property type="entry name" value="NMNAT_Archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00243">
        <property type="entry name" value="NMN_adenylyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006418">
        <property type="entry name" value="NMN_Atrans_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01527">
        <property type="entry name" value="arch_NMN_Atrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002243">
        <property type="entry name" value="PRK01153.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21342:SF0">
        <property type="entry name" value="BIFUNCTIONAL NMN ADENYLYLTRANSFERASE_NUDIX HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21342">
        <property type="entry name" value="PHOSPHOPANTETHEINE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 4 7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="6">
        <location>
            <begin position="8"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="NCBIfam" id="TIGR01527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="190" mass="21913" checksum="F92C380DBE0A0653" modified="2017-09-27" version="1">MSKLKRGLFVGRFQPVHNGHIKALEFVFSQVDEVIIGIGSAQASHTLKNPFTTSERMEMLIRALDEAGLTKKRYYLIPLPDINFNAIWATYVVSMVPRFDVVFTGNSLVAQLFREKGYEVIVQPMFRKDILSATEIRRRMIKGEPWEELVPKSVAEFIREIKGCDRIRMLATNLEKNEKELQAPIRIPEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9V9</accession>
    <name>A0A218P9V9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NADH oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_09595</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854797.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9V9"/>
    <dbReference type="SMR" id="A0A218P9V9"/>
    <dbReference type="GeneID" id="33316525"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09595"/>
    <dbReference type="OrthoDB" id="287850at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02144">
        <property type="entry name" value="iodotyrosine_dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="entry name" value="NADH Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050627">
        <property type="entry name" value="Nitroreductase/BluB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23026:SF90">
        <property type="entry name" value="IODOTYROSINE DEIODINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23026">
        <property type="entry name" value="NADPH NITROREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="FMN-dependent nitroreductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="4">
        <location>
            <begin position="8"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="187" mass="21585" checksum="A9B5FBD3D04744F3" modified="2017-09-27" version="1">MRVLELAKRRKTVRQFLPDRPPKEDILKAIKAAKEAPSGMNAQPWRFVVIDDDWLKGRIRELCEAEEEKFYSRTKGDLMAWLNAKGFKPEKPFLSEAPYLILVFGYTKAPYWLQSTWIAVGYLLLALEELGLGTVTYTPPNPKPVEELLKTPPEYKLQTILPVGYPADPKPKYERRKLEEVVSFNGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9X9</accession>
    <name>A0A218P9X9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secondary thiamine-phosphate synthase enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0047 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854839.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9X9"/>
    <dbReference type="GeneID" id="33316570"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09820"/>
    <dbReference type="OrthoDB" id="6663at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.460">
        <property type="entry name" value="YjbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001602">
        <property type="entry name" value="UPF0047_YjbQ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035917">
        <property type="entry name" value="YjbQ-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00149">
        <property type="entry name" value="TIGR00149_YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YJBQ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615:SF8">
        <property type="entry name" value="UPF0047 PROTEIN C4A8.02C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01894">
        <property type="entry name" value="YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004681">
        <property type="entry name" value="UCP004681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111038">
        <property type="entry name" value="YjbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="140" mass="16006" checksum="58C5104F076D02C9" modified="2017-09-27" version="1">MLFELEVPTSERFQIIDITDEVQRLVYRSKVKHGMAVVFTRHTTTGLFINEAESRLIEDIKAKMKELVPRGAGYSHDSLDGNAHSHIRASLLLNPEVVVPIDQAELQLGTWQRILFAELDGPRHRKVLVMICPCPEFQEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Z4</accession>
    <name>A0A218P9Z4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Alpha-amylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_01160</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854852.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Z4"/>
    <dbReference type="GeneID" id="33314836"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01160"/>
    <dbReference type="OrthoDB" id="18347at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11320">
        <property type="entry name" value="AmyAc_AmyMalt_CGTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00604">
        <property type="entry name" value="IPT_CGTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006048">
        <property type="entry name" value="A-amylase/branching_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031319">
        <property type="entry name" value="A-amylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357:SF215">
        <property type="entry name" value="ALPHA-AMYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357">
        <property type="entry name" value="ALPHA-AMYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02806">
        <property type="entry name" value="Alpha-amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00632">
        <property type="entry name" value="Aamy_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="689"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="8">
        <location>
            <begin position="17"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-amylase C-terminal" evidence="7">
        <location>
            <begin position="412"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="659"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="667"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="710" mass="79444" checksum="E7C2F0E3DFBABAF2" modified="2017-09-27" version="1">MVSAYTYLVPERGVIYQVMVDRFYDGNGSNNGPFYDPDHKNYRLYWGGDLEGLTEKLDYIKSLGVSMIWVSPLNDNINKMAHGSAPYHGYWTRDYKGIDEHFGDWNDFRELVEEAQKRGICVIVDYVPNHAGPATDGEFGALYDNGTKVTDYYEDTKNATVNPITGIREAIYHHNGNIFRWEDIPLKYANLYGLADFNQLNPWVDSYLTEGAKLFVENGACGLRIDAVKHMELGWLESFYLRLYSERPLFIYGEYYSLSTDRTEDLHELYRYSNVSPVLDIPIREDIVRTFGFVGSFETLAGELESYYSIFVYPNKQVNFLDSHDLVRFLNEAKRDDALERFHMALALTMTLPGIPVIYYGDESYLVSKDGKGDPYNRPMMVFNNSTEAARIIRTLAELRKTNDALAFGDFKTLYANYSVWAFERTFGTHKLLVVMNKGSPSNLTLSLDWPDGAYSDALYGAGMTVKGGKAMLTLPKNSFYVFHIKRPQDGPLIGSLTPYVAQPGQEVLIGGAGFRSSGRVLIGGEEAEVLSWSPSEILVRVPEVETSSAWVDVTVEAGGKRSNPAKLRYYSGSDIPALVAFNASNFTGKLWIVGNISELAEPRPLLESSTGYYFTVVPLPNGTAFSVRLYRGSAWEELEPLNVTLYGVGNRTVLLKAEPEGPPITSSPVEPSLSESPGNMPGGWPAKAIYASLAVLVVLAIGMAWRKMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Z5</accession>
    <name>A0A218P9Z5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_01370</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854856.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Z5"/>
    <dbReference type="GeneID" id="33314879"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01370"/>
    <dbReference type="OrthoDB" id="147058at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047817">
        <property type="entry name" value="ABC2_TM_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051784">
        <property type="entry name" value="Nod_factor_ABC_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43229:SF6">
        <property type="entry name" value="ABC-TYPE MULTIDRUG TRANSPORT SYSTEM, PERMEASE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43229">
        <property type="entry name" value="NODULATION PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006648">
        <property type="entry name" value="DrrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="92"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-2" evidence="6">
        <location>
            <begin position="7"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="255" mass="28310" checksum="CCAC3DBC899A22AE" modified="2017-09-27" version="1">MFFRYPLRVISSILVGVVFLLQFVYFGQAVLGGRYSALLESSTGMGDYPTYALIGYVLWWVSVSPMEAYVWGVRRELQRGTFETNVLSPSRLTGLLFGLALSWLLMDSALMVIVFAFGVSLFDIPVTIPLLLKSLPAIIASLIAFMGFGFIFAGLVMLFKNIGPFAQIFEFAMLFLSGVFFPLSVMPDWVIAISRLFPLTHSAVIVRALFAGGSYSDVGSSLLWLLALMPLYWGIGYLLFSWAERTTRVIGYGGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Z9</accession>
    <name>A0A218P9Z9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09810</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854837.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Z9"/>
    <dbReference type="GeneID" id="33316568"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09810"/>
    <dbReference type="OrthoDB" id="84940at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="104" mass="11620" checksum="7A11D1591432074D" modified="2017-09-27" version="1">MPKRVKLGHHYYYIVTVDQLNSGSFRGKNVVIEGVIEDKPVVEFLPMELPGYRTTFKVSGIRVEFSGSPCIGLGDSVRVYGRFLGDCIMASAIETERALFTTEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA10</accession>
    <name>A0A218PA10_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein-glutamine gamma-glutamyltransferase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_02980</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA10"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02980"/>
    <dbReference type="OrthoDB" id="101276at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025403">
        <property type="entry name" value="TgpA-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13559">
        <property type="entry name" value="DUF4129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="446"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="Protein-glutamine gamma-glutamyltransferase-like C-terminal" evidence="2">
        <location>
            <begin position="511"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="582" mass="64346" checksum="FFC3F33E7B817AAD" modified="2017-09-27" version="1">MRKVSILIILILIIGSVPLASGKAVQEPTQRDVFLYQYFSQVLVRFDYSLRYALVNENYSLKLANLTLSELELIKDESLYYQEKGVNSTVMRVIPPFYDFASEMVVLVQLVLEFHENPTPALASGILSTVDDMERTLDVIEGIRLMNGTEVLTFNTAKVRTHLAAIRKLVSNLPPGQGDFVIGVSDDSPILHQTITIFGSCPGNSSVTVVIEGGNSTSLLLVNSRNGLFSTNYRFDELGTYRIYAVQGGERSNNVTVTVRRIPTSFIVDDSYSAFLNSTVHLSGRLVDYYGNPLGGRKITVGNSTLTTGSDGGFSKGYFSPVATSFKVTLRFGGDATHAATMKEVIVRFTRYPVSITLDGPSEVVLGKTAVFTGTVDPALPFPITVYVAGKEYLRLTPDNGTFSFELKPNETGEFEVYASFSGNERYEKAKSNVVVLKVVPPENMMLRYIAIALLALLMVALILLERRKEGDKAPARKKPEPDVVPGEEKVPIKAETEFKIPEDVGEAYTLLRERLREALGVSESMTPREVLRALRDWELYPYLETVTKLHEKAVYGEMPLSEGETAEFVNAMENLLRGVSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA22</accession>
    <name>A0A218PA22_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription elongation factor Spt4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">spt4</name>
        <name evidence="4" type="ORF">A3L08_03375</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Stimulates transcription elongation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer composed of Spt4 and Spt5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal Spt4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_198362144.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA22"/>
    <dbReference type="GeneID" id="33315281"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03375"/>
    <dbReference type="OrthoDB" id="275101at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00949">
        <property type="entry name" value="Spt4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022800">
        <property type="entry name" value="Spt4/RpoE2_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007178">
        <property type="entry name" value="Spt4_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038589">
        <property type="entry name" value="Spt4_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006224">
        <property type="entry name" value="PRK08351.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041664">
        <property type="entry name" value="RNAP_arch_Epp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40704">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40704:SF1">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06093">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01389">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="RNA polymerase subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Spt4/RpoE2 zinc finger" evidence="3">
        <location>
            <begin position="3"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="61" mass="7092" checksum="FEDE652C993C4525" modified="2017-09-27" version="1">MKERACRHCHYITTEDRCPVCGSRDLSDEWFDLVIITDPEKSRIAEKLGVKVPGKYAIRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA52</accession>
    <name>A0A218PA52_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA-directed RNA polymerase subunit Rpo5</fullName>
            <ecNumber evidence="7">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">DNA-directed RNA polymerase subunit H</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rpo5</name>
        <name evidence="7" type="synonym">rpoH</name>
        <name evidence="9" type="ORF">A3L08_05030</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the archaeal Rpo5/eukaryotic RPB5 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="7"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854896.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA52"/>
    <dbReference type="GeneID" id="33315610"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05030"/>
    <dbReference type="OrthoDB" id="30537at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.940.20:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.20">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00025">
        <property type="entry name" value="RNApol_Rpo5_RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014381">
        <property type="entry name" value="Arch_Rpo5/euc_Rpb5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000783">
        <property type="entry name" value="RNA_pol_subH/Rpb5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020608">
        <property type="entry name" value="RNA_pol_subH/Rpb5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035913">
        <property type="entry name" value="RPB5-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007129">
        <property type="entry name" value="PRK09570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01191">
        <property type="entry name" value="RNA_pol_Rpb5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55287">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01110">
        <property type="entry name" value="RNA_POL_H_23KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 7 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4 7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNA polymerase subunit H/Rpb5 C-terminal" evidence="8">
        <location>
            <begin position="7"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="82" mass="9583" checksum="39E5E4C8368154F5" modified="2017-09-27" version="1">MATKKEFDIFMHELVPEHRILSEEEKEELLRKYRIKISQLPQIKASDPAVVALGAKPGDVLEIKRKSPTAGYYYYYRLVVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P4X8</accession>
    <name>A0A218P4X8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0128 protein A3L08_00055</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0128 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853099.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P4X8"/>
    <dbReference type="GeneID" id="33314615"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00055"/>
    <dbReference type="OrthoDB" id="84574at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00264">
        <property type="entry name" value="UPF0128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005266">
        <property type="entry name" value="UPF0128"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00703">
        <property type="entry name" value="TIGR00703 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03673">
        <property type="entry name" value="UPF0128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="222" mass="26227" checksum="5B77CA37D34F5DC0" modified="2017-09-27" version="1">MLDGYYIVENTGVVPAERRFKFKDLKAWGYDLHLGTIDGTEAYFVSKAGTREEGETYTRDGKEYHISETQREIPKNARLLARIVIEGGQPYLEFWLDTEEGNFPLAKEDPRLILHRFWTEKKFNQLEKHVGSVGLTTDFFKDRVFVKGLPLPYEEYPPKVRRVLREVRDIHRDMTGFGRFVFQYFGEEDKAHQYRLWWLLPTIHLFDVEVSNEVDKVLAMLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P4Y3</accession>
    <name>A0A218P4Y3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Carbohydrate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_00105</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853109.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P4Y3"/>
    <dbReference type="GeneID" id="33314625"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00105"/>
    <dbReference type="OrthoDB" id="26949at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050306">
        <property type="entry name" value="PfkB_Carbo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43085:SF57">
        <property type="entry name" value="CARBOHYDRATE KINASE PFKB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43085">
        <property type="entry name" value="HEXOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ05846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="275" mass="30412" checksum="BF3DD70183DC2405" modified="2017-09-27" version="1">MKCLVVGHLVRDVILRGSKAESRIGGGAYYSAIALSRFCDVEVLTSVGKDFPEGWLKELRERGIELHIIPSEKSTSYRLHYLDANTRELSLLSVAEEIASMPEGDYDMVVLNPVAGEIPPETVEEAKRKSSFVAADAQGFIRSPEIGKLRLRGINGDLFEGLKVLHADVSEINLISRFDPGEVEVLLVSNGSEEGRAYLRGREYSYLPVKVPVEESTGAGDVFLAAFSYFYTQCPFIQALKRAAAFTALFLKHRNFTFSMDEVNELAIMVGARPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5B1</accession>
    <name>A0A218P5B1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Quinolone resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_00860</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853246.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5B1"/>
    <dbReference type="GeneID" id="33314776"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00860"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17489">
        <property type="entry name" value="MFS_YfcJ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050189">
        <property type="entry name" value="MFS_Efflux_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43124">
        <property type="entry name" value="PURINE EFFLUX PUMP PBUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43124:SF9">
        <property type="entry name" value="SUGAR TRANSPORT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="209"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="335"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="363"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="7"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ05984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="390" mass="41162" checksum="A1CCA18AD05FFFB1" modified="2017-09-27" version="1">MDRTVRNIWLLNLSTFFFFLGISSVNPIVSPFSMTLGASPFLVGTLAGITSVVSLISKPVGGLVGDRGYRLEAMILGNLLSLVSGVLYVVSALTGSIWLFAFTRALHGFSMGIFFPSSLSTAVDLAPVGRVGETLGWRGMMFSLGNIIGPALGGYASDYLGFAGAFFLVVIFSAVGALFVVPVRRDVGRTLVPVEKSEEADYSELLRPYFVAASVALLFFAASYSGVTTFLPALYKELSYPQRVFGTYMMVIGIASFVTRLIGGRSADRMGPIPVSAAGLLTVITGYMLLIRFTTPPASYASAVLIGAGFGLAIPAMQLMALGNLPQRIRTMGSSVYTMFFDLGTMGGQFALGYAAGGLGYAGILRFLPALAGVALLILAVSYFVGDKNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5F0</accession>
    <name>A0A218P5F0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">8-amino-7-oxononanoate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_00985</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853267.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5F0"/>
    <dbReference type="GeneID" id="33314801"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00985"/>
    <dbReference type="OrthoDB" id="9071at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06454">
        <property type="entry name" value="KBL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000006">
        <property type="entry name" value="5-aminolevulinate synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010962">
        <property type="entry name" value="AONS_Archaea/Firmicutes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01825">
        <property type="entry name" value="gly_Cac_T_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005394">
        <property type="entry name" value="PRK06939.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF3">
        <property type="entry name" value="LD36009P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="6">
        <location>
            <begin position="39"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="395" mass="43851" checksum="9B464FE288A568B6" modified="2017-09-27" version="1">MAKLDWIREELQELKDKGLYVTIRKLESAQGPWVVVDGKKVLNMCSNNYLGLAAAPEIRYAAIRAILDYGVGAGAVRTIAGTMELHVELEEKLAKFKKREAAILLQSGYNANLGAISALLKKGEDGVFISEELNHASIIDGMRLSGAPKVIYKHLDMEDLEARLKENKDKKKKIIVSDGVFSMDGDLAPLPEMAELAEQYDAILYIDDAHGEGVLGDSGRGIVDHFKLHDRVDFEMGTLSKAFGVIGGYVAGPEEAIEYLRQRARPFLFSSAPNPPDVAAAIAAVEILQRSDELVKKLWDNTHFLQNGLRELGYDLGNTKHPITPVMLYDEKLAQEFSRRLYDEYNIFAQAIVYPTVPLGTARIRLEPSAAHSKEDLQYVIDAFEDLGKKTGFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5G3</accession>
    <name>A0A218P5G3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Probable GTP 3',8-cyclase</fullName>
            <ecNumber evidence="11">4.1.99.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Molybdenum cofactor biosynthesis protein A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">moaA</name>
        <name evidence="13" type="ORF">A3L08_00960</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>GTP + AH2 + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49576"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.1.99.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="11">Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the radical SAM superfamily. MoaA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.22" evidence="11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5G3"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00960"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061798">
        <property type="term" value="F:GTP 3',8'-cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21117">
        <property type="entry name" value="Twitch_MoaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01225_A">
        <property type="entry name" value="MoaA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040064">
        <property type="entry name" value="MoaA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013485">
        <property type="entry name" value="MoaA_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010505">
        <property type="entry name" value="MoaA_twitch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050105">
        <property type="entry name" value="MoCo_biosynth_MoaA/MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02668">
        <property type="entry name" value="moaA_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001199">
        <property type="entry name" value="PRK00164.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960:SF0">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SYNTHESIS PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06463">
        <property type="entry name" value="Mob_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01383">
        <property type="entry name" value="cyclic_pyranopterin_phosphate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01386">
        <property type="entry name" value="main_SPASM_domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 11" id="KW-0408">Iron</keyword>
    <keyword evidence="6 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="12">
        <location>
            <begin position="6"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="257"/>
            <end position="259"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="308" mass="35662" checksum="6FB1AB613058BFA1" modified="2017-09-27" version="1">MVLYDRFGRPVTNLRISLTQDCNFRCFFCHREGQRFNVGNEMTPEEIERLVRIASHLGIKKVKLTGGEPTVRDDILEIVERIKPYVVDLSMTTNGSRLKELAKPLAKAGLDRVNVSLHSLKPDVYKRITGVDMLETVLEGIEEAVKYLSPVKLNMTVMKGLNEGEIWDMVDFAAKTGTILQLIELEAPREMTKTTFFRKYFYPLKHVERELEKRAVETRERRMHRRKKYFVPTDYGIAEVEVVRAMHNTVFCANCTRLRVTSNGMFKTCLLRNDDLINFLSAMRNGASDAEIAEIFKKAVLMREPYWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5H4</accession>
    <name>A0A218P5H4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cardiolipin synthase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_01055</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5H4"/>
    <dbReference type="GeneID" id="33314815"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01055"/>
    <dbReference type="OrthoDB" id="100026at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="CLS_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13396">
        <property type="entry name" value="PLDc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Cardiolipin synthase N-terminal" evidence="7">
        <location>
            <begin position="23"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="89" mass="10226" checksum="521300C97E3DE7F9" modified="2017-09-27" version="1">MGEMAFFEIVWILGLTLMVAQLIALVWVIYDVMTKQKKMPDVEKVIWVLVAFFTTILGAIIYYILVKRTGKYEEGPGEHMENPDGPIVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5H5</accession>
    <name>A0A218P5H5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Antitoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_01105</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Antitoxin component of a type II toxin-antitoxin (TA) system.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UPF0165 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5H5"/>
    <dbReference type="GeneID" id="33314825"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01105"/>
    <dbReference type="OrthoDB" id="116241at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1150.10">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="AF2212-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01954">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141694">
        <property type="entry name" value="AF2212/PG0164-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-1277">Toxin-antitoxin system</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU368051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="70" mass="8085" checksum="797B4369A82548E0" modified="2017-09-27" version="1">MRDGGGREIIEAIYEDGVLKPLKKPKLREHSKVRIKIIPEDVDDLLDSLVIEKVGSIDYKRLKEAYYESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5J2</accession>
    <name>A0A218P5J2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5J2"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01210"/>
    <dbReference type="OrthoDB" id="105894at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="1">
        <location>
            <begin position="69"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="183" mass="21188" checksum="560A8840CAA6E512" modified="2017-09-27" version="1">MDIFNKLIQRKFRGIAGERYEEIAKRYREFLLLPEEFVLPEVRSILMPIDRFSGEIPESLYETLSAYIEAKVTVVYISEKKTLKIIEETLGREEAERLRNAKMEFAEKLSGEIASRLEGLGLRVERRHFLGSKSDDIISLLKDEDFDLLVVSRSYGSEVTKTSPISPIVLRIVQHVEKPVIVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5L0</accession>
    <name>A0A218P5L0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alkylhydroperoxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_01290</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853322.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5L0"/>
    <dbReference type="GeneID" id="33314863"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01290"/>
    <dbReference type="OrthoDB" id="111898at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004675">
        <property type="entry name" value="AhpD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00778">
        <property type="entry name" value="ahpD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33930">
        <property type="entry name" value="ALKYL HYDROPEROXIDE REDUCTASE AHPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33930:SF2">
        <property type="entry name" value="BLR3452 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxymuconolactone decarboxylase-like" evidence="2">
        <location>
            <begin position="30"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="119" mass="13133" checksum="3311BC56AED34DD1" modified="2017-09-27" version="1">MGEEDVMVKLKEIEALLDKLGKEHPKEISAFSRFLRETLDNKALTTREKELIAVALGIAAGCEWCIYLHTQKALEAGAKPEELLEAGLVAVLMAGGPALMHLIPLTKAIEAFKKDYEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5M0</accession>
    <name>A0A218P5M0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_01415</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
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            <taxon>Thermococcales</taxon>
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        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06082.1"/>
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    <dbReference type="GeneID" id="33314888"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01415"/>
    <dbReference type="OrthoDB" id="26019at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050638">
        <property type="entry name" value="AA-Vitamin_Transporters"/>
    </dbReference>
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        <property type="entry name" value="EamA_dom"/>
    </dbReference>
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        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32322:SF2">
        <property type="entry name" value="EAMA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32322">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
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            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
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    <feature type="transmembrane region" description="Helical" evidence="5">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
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            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
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            <begin position="6"/>
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    </feature>
    <feature type="domain" description="EamA" evidence="6">
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            <begin position="145"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="277" mass="30263" checksum="383D25A0B3F273EF" modified="2017-09-27" version="1">MSKKHAVGAVLLWSTVASAFKLSLRYLTPLQLLFYASLTSLIVFGALYSREFSLRRGNLRSAYLGLINPLLYYIVLFSAYDRLPAQEAQALNYTWPLMLVLLSIPLLGKRPGARTVLGLSIGFLGALVVATRGDITDLNFSDPVGFALGLGSAVIWASYWLLNLRDERPLVEKMFWNFLFGFAYVFTALALSGGLALPPLKGLVGAVYVGLFEMGVTFLLWYKAVEGDMAFASNLAYLVPFLSLFFISLVVGESIAPATVLGLAMIVGGILLGRRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5M5</accession>
    <name>A0A218P5M5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ubiquinone biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_01425</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853346.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5M5"/>
    <dbReference type="GeneID" id="33314890"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01425"/>
    <dbReference type="OrthoDB" id="147504at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050508">
        <property type="entry name" value="Methyltransf_Superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023576">
        <property type="entry name" value="UbiE/COQ5_MeTrFase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42912">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42912:SF96">
        <property type="entry name" value="METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01184">
        <property type="entry name" value="UBIE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="4">
        <location>
            <begin position="41"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="202" mass="23608" checksum="F0EBA68735DCD1EA" modified="2017-09-27" version="1">MSFREKYARLGERYERIDGPLERFFDTLRRKAARFVSGEVLEVGVGTGFMLPYYSREIELHAIDAVPEMLEVAKKRAEELGLNARFYVMDAERLEFPDESFDTVVSAFVFCTIPNPERAMKELYRVLKPGGRAVFLEHTKSDCRLVNWLLLKPLDLFLGWLIGDNTLRETHRLVEKYFDVEHEESHYSGVVRLMIARKPEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5N5</accession>
    <name>A0A218P5N5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Na-dependent transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_01300</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Potential transporter for phosphate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853324.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5N5"/>
    <dbReference type="GeneID" id="33314865"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01300"/>
    <dbReference type="OrthoDB" id="101311at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101:SF80">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="131"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="292"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="320" mass="32817" checksum="96022E58FF6DA0A6" modified="2017-09-27" version="1">MEGLELAAVAVAFYIAWNIGSNDSANAMGTAVGAGILSFRQATLTIAIFVLMGAYLQGYKVMKTVGKGIVPEGHLTMEMAVIALLAAGVWVTIATVKGLPVSTTQAIVGGVIGVGLATHAPVNWWTLTKIASAWVISPILSGIFAIVLFKFYSWVISRIKSVSTIETLYKALAILGGSYMAFNFGTNEVANASGPLVGAGFMEPKVAGIFGALALATGALTFSYAVMHTVGKKITALGPVSAFAAQFGSAISVSLANFFGLPVSSSQSIVGGVVGVGLLAGQGVKKSVIRDIIFGWVATPLTAIFISLAISKAFAFAGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5R5</accession>
    <name>A0A218P5R5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_01785</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 30 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853409.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5R5"/>
    <dbReference type="GeneID" id="33314962"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01785"/>
    <dbReference type="OrthoDB" id="43567at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.80">
        <property type="entry name" value="NADH:ubiquinone oxidoreductase, 30kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037232">
        <property type="entry name" value="NADH_quin_OxRdtase_su_C/D-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001268">
        <property type="entry name" value="NADH_UbQ_OxRdtase_30kDa_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10884:SF14">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10884">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00329">
        <property type="entry name" value="Complex1_30kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143243">
        <property type="entry name" value="Nqo5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase 30kDa subunit" evidence="2">
        <location>
            <begin position="48"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="189" mass="21450" checksum="90EA8405FB826B0A" modified="2017-09-27" version="1">MNDNPVTEANEVKEPTKAEKVAKVIAERFPEAEVQVKTNKWGRQRVWVRIPREKYRELMEFVKTLDGEAHYSIGIEQDWGDELGFLSHLLIYYDDAPAVSLLVDVHAPKDDPVIPDISDIFPIALQFEREGMEMVGLDFEGAPDKRKLFLPDDFPEGIYPLRLDDKGVSEEMVHNAGHPYLIKKGGGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5S8</accession>
    <name>A0A218P5S8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cation:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_01770</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853406.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5S8"/>
    <dbReference type="GeneID" id="33314959"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01770"/>
    <dbReference type="OrthoDB" id="371891at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050586">
        <property type="entry name" value="CPA3_Na-H_Antiporter_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001516">
        <property type="entry name" value="Proton_antipo_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006240">
        <property type="entry name" value="PRK08376.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42703:SF1">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER SUBUNIT D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42703">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00662">
        <property type="entry name" value="Proton_antipo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01434">
        <property type="entry name" value="NADHDHGNASE5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="344"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="387"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="424"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="465"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="NADH-Ubiquinone oxidoreductase (complex I) chain 5 N-terminal" evidence="8">
        <location>
            <begin position="72"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="7">
        <location>
            <begin position="134"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="511" mass="54672" checksum="165BD546EBF14F0D" modified="2017-09-27" version="1">MMVLPYLIIIPLLGAFSMPIVSLAGRKAREAWAVLISGATLAVGSWVFYHVYENGTILYTLGAKSPLGQGVNFPIRIVWEVDLFGALMVLMVTLVSFMAVLYSIGYMRHDTGLDKYYALIITLELGMLGIAITGDLFNFYVFLEIMSIASYALVAFRNDTWEGIEAGIKYMFVGSLASSFVLLGIALLYGQYGTLTMGYLALKLSESLTITAKVALALFIAGLLFKSGASPVHMWLADAHPAAPSSISAMLSGLVIKIGGLYALARIAFSIYGASVSVKTVGWLIIIFACITLIVGNAMAVIQTDMKRLLAYSSVGQVGYILLGLGIGLAAYGSQTGEVALAGAIYHTFNHALMKALLFLIAGAVIHQLGTRNLNELSGLAKTMPKTTFAFLIGAAAIVGMPPLNGFASKWLIYESSALFNPVLAAIAVIGTAFCTAAYVRVLYTFFGKPSEKVMEAKDPEASMLWPMIILTIAIIVMGLFPWQISDKVMIPAAKALENQLSYIATLMGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5V0</accession>
    <name>A0A218P5V0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Enolase</fullName>
            <ecNumber evidence="6">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">2-phosphoglycerate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 12" type="primary">eno</name>
        <name evidence="12" type="ORF">A3L08_01875</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reversible conversion of 2-phosphoglycerate (2-PG) into phosphoenolpyruvate (PEP). It is essential for the degradation of carbohydrates via glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(2R)-2-phosphoglycerate = phosphoenolpyruvate + H2O</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds a second Mg(2+) ion via substrate during catalysis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Mg(2+) is required for catalysis and for stabilizing the dimer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell surface</location>
        </subcellularLocation>
        <text evidence="6">Fractions of enolase are present in both the cytoplasm and on the cell surface.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853426.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5V0"/>
    <dbReference type="GeneID" id="33314980"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01875"/>
    <dbReference type="OrthoDB" id="8680at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.10:FF:000001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF1">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
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        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00178">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 6 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Enolase N-terminal" evidence="11">
        <location>
            <begin position="7"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="10">
        <location>
            <begin position="140"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6 7">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6 7">
        <location>
            <position position="340"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="367"/>
            <end position="370"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="369"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="430" mass="46700" checksum="210AF665B7D9A6BF" modified="2017-09-27" version="1">MENPFEITGVVAREILDSRGNPTVEVEVYTPISMGRAAVPSGASTGTHEALELRDGGTRYHGKGVRRAVENVNKIIAPEIIGMDVTWQRDIDNLMLELDGTENKSNLGANAILGVSLAVAKAAANALGLPLYQYIGGTNAYVMPVPMSNVINGGVHAGNELDFQEFMIMPVGADSFREAIRWVSETYHVLKKVIAERYGKNAINVGDEGGFAPPMKEVTEPLDVLIKAIEEAGYKPGDEIAFALDAASSEFFHPDKGKYVVAGKEYDKGELLELYRELVGTYPIVSIEDPFHEEDWEGFAMITKELGSKIQIVGDDLFVTNPKRIRKGIELGAANALLLKVNQIGTLSEAIDAAYTAFRAGYGVVVSHRSGETEDATIADLAVALNAGQIKTGAPARSDRNAKYNQLIRIEEELEGIALYPGRKFRNPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5V2</accession>
    <name>A0A218P5V2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Thioredoxin-disulfide reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_01840</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
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            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
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    <dbReference type="GeneID" id="33314973"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01840"/>
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    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:removal of superoxide radicals"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
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        <property type="entry name" value="Ferredoxin-NADP_redctase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008255">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_2_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005982">
        <property type="entry name" value="Thioredox_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01292">
        <property type="entry name" value="TRX_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48105">
        <property type="entry name" value="THIOREDOXIN REDUCTASE 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FADPNR"/>
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    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PYRIDINE_REDOX_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="6">
        <location>
            <begin position="19"/>
            <end position="310"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="330" mass="35972" checksum="7EA69939CF32A9AA" modified="2017-09-27" version="1">MFSLGGFSRGGEYENKTWDVLIIGAGPAGFTAAIYAARYGLDTLIISKDLGGNMALTDLIENYPGFPEGISGSELANRMHEHVKKLGVDVVFDEVERIDPAECAYYEGPCKFTVKTKNGKEYKGKTIIIAVGAAPRKLHVPGEEEFTGRGVSYCATCDGPLFKGKKVIVVGGGNTALQEALYLKSIGVDVTLVHRRDQFRADKILQDRFKESGIPAILNTVVTEIKGTNKVEAVKLKNRVTGEEWEMPVDGVFIFIGYEPKTDFVKHLGITDEYGYIPVDMHMRTKVPGIFAAGDITNVFKQIAVAVGQGAIAANSAKELLEEWNSKVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5X1</accession>
    <name>A0A218P5X1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MrpA C-terminal/MbhE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_01755</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853404.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5X1"/>
    <dbReference type="GeneID" id="33314956"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01755"/>
    <dbReference type="OrthoDB" id="371891at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046806">
        <property type="entry name" value="MrpA_C/MbhE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20501">
        <property type="entry name" value="MbhE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="MrpA C-terminal/MbhE" evidence="2">
        <location>
            <begin position="43"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="101" mass="11389" checksum="37DFB42303AF2DCC" modified="2017-09-27" version="1">MKRTLAYLSLLFILGVLLYVANPNYGLKFGPGGEEWKALRYTDDYYITHGLEEVGGTNIVTDIVFDYRGYDTIGEATVLFTAIAGAVALFRPWRRDGNEHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5X5</accession>
    <name>A0A218P5X5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1884 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01855</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853423.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5X5"/>
    <dbReference type="GeneID" id="33314976"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01855"/>
    <dbReference type="OrthoDB" id="86177at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.10">
        <property type="entry name" value="hypothetical protein PF0899 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014418">
        <property type="entry name" value="ENCP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036216">
        <property type="entry name" value="ENCP4_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041191">
        <property type="entry name" value="encap_f4b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08967">
        <property type="entry name" value="ENCP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004604">
        <property type="entry name" value="UCP004604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111057">
        <property type="entry name" value="Hypothetical protein PF0899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="94" mass="10756" checksum="A6F3ADA16C2A6ED3" modified="2017-09-27" version="1">MKELKDLLHKAVNELKSEGLEPDIILVGPQFIEHAAEVLRGCGFKIYKIEELGYDAVVADSKYLGQMKRASRRISIEPLLAENEMWEELKKLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Y3</accession>
    <name>A0A218P5Y3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_01910</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853433.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5Y3"/>
    <dbReference type="GeneID" id="33314987"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01910"/>
    <dbReference type="OrthoDB" id="14434at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF4">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="156"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="305" mass="35891" checksum="743BAC50D492848F" modified="2017-09-27" version="1">MPEPVMEELELLVEILDRHPLESLRRIAESEGIEYYRLKRLHDKYYGKYLTVSASANIRRIGLRSFVAFLSVSPDRLMEVASRVTRNPFIGYANPAFGFKNGLSLTIYVPDKQKKLVGKMLSKYSDDYEYYEVRAYPYSGDEDFGDWYLSYDYAVLLDILKWDARTPITEISRRLGKSRPTVRYMIKRLQEEGIIVKFAPLVDMNIHNRGVIGLTKHLDDEVLERFKEYEITVGVLPGYGYILEWFFSSKEDLGSKVLEFSNYVEKLLIEYFDPVFKEMNDRNLKDRYQRMVKEDGNGYRSILEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Y4</accession>
    <name>A0A218P5Y4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rubrerythrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01810</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853414.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5Y4"/>
    <dbReference type="GeneID" id="33314967"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01810"/>
    <dbReference type="OrthoDB" id="102142at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01045">
        <property type="entry name" value="Ferritin_like_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rr_diiron-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33531:SF10">
        <property type="entry name" value="BLR7895 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33531">
        <property type="entry name" value="RUBRERYTHRIN SUBFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rubrerythrin diiron-binding" evidence="1">
        <location>
            <begin position="16"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="160" mass="18775" checksum="BF3B658B685DE430" modified="2017-09-27" version="1">MVVEILNEIRKLNERELLSYWIKGEYEEAETYWKLAERAKELGLPENVGETFKRLGNESKRHGDELLKIYRDEYGDDLVEVNVPNVEALNVLGKFWKVEDLEDVLKIAMESEKLAETIYRKLADETENPKLRETYLRLAEIEKGHYEALASLWKSLGLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P608</accession>
    <name>A0A218P608_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Type II secretion protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_02180</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853483.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P608"/>
    <dbReference type="GeneID" id="33315041"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02180"/>
    <dbReference type="OrthoDB" id="33500at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01130">
        <property type="entry name" value="VirB11-like_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050921">
        <property type="entry name" value="T4SS_GSP_E_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486">
        <property type="entry name" value="TWITCHING MOTILITY PROTEIN PILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486:SF15">
        <property type="entry name" value="TYPE II_IV SECRETION SYSTEM ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="352"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="619" mass="69333" checksum="65D63A837C95400F" modified="2017-09-27" version="1">MFDEKKKKKEVSWIDEILNGEDNLLESMLNGEKKKGTKEKEELPLLGSGGGVNLEEILSTPTTPEEAVGSRPTGADILGEILTKEEPVEKPRPRPKPRPPSTLQDILGSSARVEEASYAGKAEVLDAYGNVRIVKVKGEPVPIYELRLPELSKDEEELLARIRDRAITELQIDPTAFPNPEERRRVFMNAVKQVIRSEAPTFSEGRIEVLADMIVQSMIGYGKLDPLVHDDNLEEIMVIGTNKPVYVWHRRFNMCKTNIVFPNDKEILNIIERIAREVGRRIDQQSPLLDARLPDGSRVNATIPPISLDGPTITIRKFRKDPLTIIDLIKYGTMNTDIAALLWIFVDGLGVKPANVLVAGGTGSGKTTTLNSLGMFIPPSERVITIEDTAELQLPVEHWVRLETRPPNVEGKGEITMDDLVKNTLRMRPDRIIVGEVRGPEARTMFTAMNTGHDGCMGTIHANSARETIVRLESPPMSVPRIMIPALDIILMQVRFHSRKKGTIRRVTEIAEVSGIEAESIQLNTLYKYDPAKDELVPTGVPSRALNNLAQHTGMSIAELELEKEKRKIILEWMVEQGIRGIDKVGHYIRQFYIDEDALLKRIEAEGSAEMSRKVRNVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P609</accession>
    <name>A0A218P609_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P609"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02075"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="212" mass="23814" checksum="9201BDEA104738F9" modified="2017-09-27" version="1">MNYDTGECTIFGMMVYNTPCRVFSMITFSLGMAMLILFLAGRLRASFAVGIAYYSVTFFAAWTWALSAKVLASLGIFLSLIGLYSSPRDTETSRSLREATSLVIYALVFLIFLLSAVSYIFYFSWVCWDAYSGAVAFLCSLISYILIGAGLVSPLLWRKRLRIPALVALLPAVLFLDFAVKLLQFSRLLWALVAVLFAMELWGRCENVMRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P624</accession>
    <name>A0A218P624_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="2 6">2.7.7.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">UDP-glucose pyrophosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_02305</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>alpha-D-glucose 1-phosphate + UTP + H(+) = UDP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the UDPGP type 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9" evidence="2 6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853505.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P624"/>
    <dbReference type="GeneID" id="33315066"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02305"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-alpha-D-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02541">
        <property type="entry name" value="UGPase_prokaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005771">
        <property type="entry name" value="GalU_uridylyltTrfase_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01099">
        <property type="entry name" value="galU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43197">
        <property type="entry name" value="UTP--GLUCOSE-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43197:SF1">
        <property type="entry name" value="UTP--GLUCOSE-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="7">
        <location>
            <begin position="5"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="292" mass="32907" checksum="D19C2C0245D5D253" modified="2017-09-27" version="1">MRVRKAVIPAAGLGTRMLPITKSMPKEMLPIVDKPVIHYVVEEAIKAGIDDILIITGKGKRAIEDYFDRSFELEYYLREKGNLGQLRQVEEIGEMVDIYYVRQKKPLGLGDAILHAEKHVNGEPFAVLLGDDIIISEKPGIKQLIEVAEKKDAPVIGVEEVPWELVSRYGIVEGELVSRSLYRIKDMVEKPSPSEAPGNIAIIGRYILPPEIFEFLRRTPPGKGGEIQLTDALREMLKEQDMFAKVIDGERYDVGSRAGFIRANLEISLSRGDLREEVIKLMKSVLREVQDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P633</accession>
    <name>A0A218P633_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">KaiC-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_02280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853501.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P633"/>
    <dbReference type="GeneID" id="33315061"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02280"/>
    <dbReference type="OrthoDB" id="103620at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="247" mass="28396" checksum="62AEEAC9370BD1DA" modified="2017-09-27" version="1">MEVLSTGIPVLDEALGGGLLEDSNLLIIYDTYSNGWTLAFEVLRNRIREGDFGVIIDSVLPLTPLKMELGMLNFDVEKEGRAGNLAILDIFSSFHKLSYDEDYVYTDSTLDSETFLPKYLHLYRRVLKERIHERRPIGIDVTVDGLAFLLGEESTIKLFQSLMALKERAKMEEKRKRPINIFLLNRGRASDKLVSWISLYSQYVIEFCSSADSIKEKMVIRKSTLPEFKPREGGYRFRIENGRVIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P641</accession>
    <name>A0A218P641_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_02400</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853521.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P641"/>
    <dbReference type="GeneID" id="33315085"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02400"/>
    <dbReference type="OrthoDB" id="92044at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49344">
        <property type="entry name" value="CBD9-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="459" mass="52058" checksum="C95E57E9DD41EC56" modified="2017-09-27" version="1">MMIETWESDLKGWIVSNDNTFTDVTSEIKRKYSKIDDDWIVVEIAIPISIFGDVTNPALKVSFVLTGGSDGTLQWFSDVAPDQDVPSWNKWGGAVAPAILTRFIFWSPDQPLEIQIVDTEFSYDQYILNKKATLIASFIGLNSFYGQKKFEEYYSKYQDINKELEEYYIPPEVGDKIYEYNNEVTDILKQYQEGITKISLPGQAFIGAFKIYRSYTDLREIVLEMEEILENAKRGQYERKEYMEELAKNLTKTIDGNLDDWSVEPVAVDDVGYGQDGANLKALYVDYDDQFLYIAITTENKASWRIAYGVSLDYKDGGYTTGQDSWGRKVDFSRGIDAQLYFFWNGEFFDDKGTSTITSAQLVLWNGAGWEYRDLQWVGFYNYTGSADDGLQTLEIAIPWSELGGKPKQISVVAYVTGQGVGDSAVDSLPLQDAVKDTDPGQEWGDADTFTEFATVTIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P657</accession>
    <name>A0A218P657_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable L-aspartate decarboxylase</fullName>
            <shortName evidence="7">ADC</shortName>
            <ecNumber evidence="7">4.1.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mfnA</name>
        <name evidence="9" type="ORF">A3L08_02375</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the decarboxylation of L-aspartate to produce beta-alanine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-aspartate + H(+) = beta-alanine + CO2</text>
            <dbReference type="Rhea" id="RHEA:19497"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="EC" id="4.1.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the group II decarboxylase family. MfnA subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.11" evidence="7"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853517.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P657"/>
    <dbReference type="GeneID" id="33315080"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02375"/>
    <dbReference type="OrthoDB" id="56891at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004068">
        <property type="term" value="F:aspartate 1-decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000125">
        <property type="entry name" value="Probable L-tyrosine/L-aspartate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01610">
        <property type="entry name" value="MfnA_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050477">
        <property type="entry name" value="GrpII_AminoAcid_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020931">
        <property type="entry name" value="MfnA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021115">
        <property type="entry name" value="Pyridoxal-P_BS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03812">
        <property type="entry name" value="tyr_de_CO2_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42735">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42735:SF6">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE LYASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00392">
        <property type="entry name" value="DDC_GAD_HDC_YDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="4 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7 8">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="386" mass="42573" checksum="AD8A0F88E407EE24" modified="2017-09-27" version="1">MFPGQGASEEDVLRELEEKTAEDLTFDSGRILGSMCTYPHPFAVKVVQRYIDRNLGDPGLHVGSQKIEKEAVDMLANLLGLEKGYGHIVSGGTEANILAVRAFRNLSDVEKPELILPESAHFSFIKAGEMLGVKLIWAKLRKDYSVDVRDVESKITDNTIGIVGIAGTTGLGVVDDIPALSDLALDYGLPLHVDAAFGGFVIPFAKALGYDIPDFDFRLKGVKSITIDPHKMGMVPIPAGGIIFREKKYIDAISVLAPYLAGGKIWQATITGTRPGASALAVWAMIKHLGFEGYKEVVKRAMELSRWFAGELKKIPSVYLIREPVLNIVSFGAEELERVEEELKRRGWGISAHRGYIRVVVMPHVRREHLEEFLRDLREIVAGLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P663</accession>
    <name>A0A218P663_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sulfate adenylyltransferase</fullName>
            <ecNumber evidence="7">2.7.7.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">ATP-sulfurylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Sulfate adenylate transferase</fullName>
            <shortName evidence="7">SAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">sat</name>
        <name evidence="10" type="ORF">A3L08_02355</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>sulfate + ATP + H(+) = adenosine 5'-phosphosulfate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18133"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="EC" id="2.7.7.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the sulfate adenylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.4" evidence="7"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853514.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P663"/>
    <dbReference type="GeneID" id="33315076"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02355"/>
    <dbReference type="OrthoDB" id="6358at2157"/>
    <dbReference type="UniPathway" id="UPA00140">
        <property type="reaction ID" value="UER00204"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004781">
        <property type="term" value="F:sulfate adenylyltransferase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070814">
        <property type="term" value="P:hydrogen sulfide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00517">
        <property type="entry name" value="ATPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.10">
        <property type="entry name" value="Sulfate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00066">
        <property type="entry name" value="Sulf_adenylyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025980">
        <property type="entry name" value="ATP-Sase_PUA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020792">
        <property type="entry name" value="SO4_adenylyltransferase_pro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024951">
        <property type="entry name" value="Sulfurylase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002650">
        <property type="entry name" value="Sulphate_adenylyltransferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003166">
        <property type="entry name" value="PRK04149.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00339">
        <property type="entry name" value="sopT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43509">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43509:SF1">
        <property type="entry name" value="SULFATE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01747">
        <property type="entry name" value="ATP-sulfurylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14306">
        <property type="entry name" value="PUA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 7 10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-sulfurylase PUA-like" evidence="9">
        <location>
            <begin position="4"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Sulphate adenylyltransferase catalytic" evidence="8">
        <location>
            <begin position="164"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="379" mass="43626" checksum="5A50541C0E73D92F" modified="2017-09-27" version="1">MVSKPHGGKLVRRLVAERTRERILSEQKEYPRVNIDHGRAVDLENIAHGVYSPLKGFLTSDDFHSVLDHMRLSDDTPWTIPIVLDVREKTFDEGDAVLLYHDGLPIARMHVEEIYTYDKKEFAVKVFKTDDPAHPGVVRVMSMGDYLVGGEIELLNELPNPFAKYTLRPVETRILFKELGWKTIVAFQTRNAPHVGHEYVQKAALTFVDGLFINPVLGKKKKGDYRDEVIIKAYEALFKHYYPKDAATLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGVGDYYGPYEAWEMFDNFPDLGITPMFIREAFYCRKCGGMVNAKICPHSEEFHVHISGTKLRKMIMAGEQPPEYMMRPEVYEVIRSFENPFVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P688</accession>
    <name>A0A218P688_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Oligopeptide ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_02675</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853574.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P688"/>
    <dbReference type="GeneID" id="33315141"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02675"/>
    <dbReference type="OrthoDB" id="18209at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000016">
        <property type="entry name" value="Oligopeptide ABC transporter ATP-binding component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050319">
        <property type="entry name" value="ABC_transp_ATP-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43776:SF8">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43776">
        <property type="entry name" value="TRANSPORT ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="335" mass="38346" checksum="FF85B5701128BB3D" modified="2017-09-27" version="1">MAEPVLKVENLKKYFPIKRGFVDTIKGAPQRKVHAVDGISFEIFKQQVFALVGESGCGKSTTGKLIVKLLEPTDGKIYLEGNDVTHIKTREEILNYRRHVQMIFQDPFSSMNPRFRIFDILEEPLLIHGIGETKAEREELIYKALEMVKITPPEDYVGRFPHMLSGGQRQRVAIARALILNPTFIVADEPVSMLDVSIRAEILELMKELKEKMGVTYLYITHDMSTARYFADWMAVMYLGRIVEMGPAKRVIDNPLHPYTRALLTAVPEPKPERRNVIKELPIKGEVPNAVDIPPGCRFHPRCIYAQKGLCDTKHPQLVEYEHNHWAECHLVGKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6D0</accession>
    <name>A0A218P6D0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Phosphoglucosamine mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">A3L08_02790</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853596.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6D0"/>
    <dbReference type="GeneID" id="33315164"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02790"/>
    <dbReference type="OrthoDB" id="10363at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008966">
        <property type="term" value="F:phosphoglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03087">
        <property type="entry name" value="PGM_like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000001">
        <property type="entry name" value="Phosphoglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.50:FF:000009">
        <property type="entry name" value="Probable phosphoglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024086">
        <property type="entry name" value="GlmM_arc-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03990">
        <property type="entry name" value="Arch_GlmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43771">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43771:SF1">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="9">
        <location>
            <begin position="2"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="10">
        <location>
            <begin position="148"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="11">
        <location>
            <begin position="250"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase C-terminal" evidence="8">
        <location>
            <begin position="363"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="456" mass="50464" checksum="F9F5B6A25DA66E40" modified="2017-09-27" version="1">MKLFGTAGIRGTLWEKVTPELAMRMGMAIGTYVDGESVAVARDGRTSSVMLQSAMISGLLSTGKEVLDLGLIPTPALAWATREHADGGVMITASHNPPTDNGIKVFNGDGTEFYVEQERELEELYFSGSFRKANWDEIKSVKPIEVIDDYIKAVLDFVNHETSLKVLYDGANGAGSVLAPYLLREMGAKVISVNAHVDGHFPGRKPEPRYENIAYLGELARELGVDLVIAQDGDADRIAVFDEKGNYLTEDTVIALFAKRYVEEHGGGTVVVSIDTGSRIDHVVEEAGGRVVRIPLGQPHDGIKKYGAIFAAEPWKLVHPKFGPWIDSFVTMGLLIKMIDEEGKPLSEIARENVPEFYLTKKNVPCPDELKKRVVESSAKVVEEKLGDEIKEILTISGYRFNLKDRSWVLVRPSGTEPKIRVVVEAPSEKRRDELFEFAYRTVREAVEKAKKNKGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6D2</accession>
    <name>A0A218P6D2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Peptide transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_02665</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853572.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6D2"/>
    <dbReference type="GeneID" id="33315139"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02665"/>
    <dbReference type="OrthoDB" id="312811at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43839">
        <property type="entry name" value="OPPC IN A BINDING PROTEIN-DEPENDENT TRANSPORT SYSTEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43839:SF1">
        <property type="entry name" value="OPPC IN A BINDING PROTEIN-DEPENDENT TRANSPORT SYSTEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="289"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="332"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="401"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="453"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="283"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="489" mass="53866" checksum="ED8E35FDD88F3D46" modified="2017-09-27" version="1">MRWVDVKEGIKEFLEEFKREKTGIAGVILLVLLVIVALTAPYTTMPDLPEKWRSSAYWEDNPKNVPPTWYNMFTSQKLVPQQVYYVDDLKIGHPSDTETVIEADYTFPEGYYFGPQGIIIKNINVTLNQASQAPTYSIYLKRPDGKTVVLVKNKPLTSSTTIAVGRDATISANIYVWLVNVTEGKELDPIQAQMDPIVIMNINTLVSTAFAKVEPGMKAEDVINNPEPLPGNYKLVLDIKNPAPDQNKVILDEMKVTFLGRSYGMMGTDYLGRDLWAGIIWGSRVSLTIGILVSVLSTIIGLTYGVTSAYLGGNMDELMMRINEIFASIPSLPILILIGATVGHVTLMFIVFLLVIFGWMGIARIARSMALQIKEQTYIEAARALGAGNGRIIFKHILPQLLPYAFATIALSVPGAVISEASLSFLGIGDPTAVTWGQILNAAQTQSATTKGYWWWVLPPGLGIAVVGLTFVLIGTALDRILNPRLRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6E8</accession>
    <name>A0A218P6E8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA 2',3'-cyclic phosphodiesterase</fullName>
            <shortName evidence="2">RNA 2',3'-CPDase</shortName>
            <ecNumber evidence="2">3.1.4.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_02925</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-phosphomonoester.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + H2O = a 3'-end 2'-phospho-ribonucleotide-RNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11828"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:17353"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:173113"/>
            <dbReference type="EC" id="3.1.4.58"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 2H phosphoesterase superfamily. ThpR family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.58" evidence="2"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853620.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6E8"/>
    <dbReference type="GeneID" id="33315191"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02925"/>
    <dbReference type="OrthoDB" id="44091at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004113">
        <property type="term" value="F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008664">
        <property type="term" value="F:RNA 2',3'-cyclic 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1140.10:FF:000009">
        <property type="entry name" value="RNA 2',3'-cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01940">
        <property type="entry name" value="RNA_CPDase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014051">
        <property type="entry name" value="Phosphoesterase_HXTX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004175">
        <property type="entry name" value="RNA_CPDase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02258">
        <property type="entry name" value="2_5_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35561">
        <property type="entry name" value="RNA 2',3'-CYCLIC PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35561:SF1">
        <property type="entry name" value="RNA 2',3'-CYCLIC PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02834">
        <property type="entry name" value="LigT_PEase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="LigT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoesterase HXTX" evidence="3">
        <location>
            <begin position="7"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoesterase HXTX" evidence="3">
        <location>
            <begin position="98"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HXTX 1" evidence="2">
        <location>
            <begin position="40"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HXTX 2" evidence="2">
        <location>
            <begin position="125"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="184" mass="21022" checksum="A78C5FF7B06170BF" modified="2017-09-27" version="1">MRAFIAVEVSDEVRDNLLKAQERIGSKAAKIKFVERENFHVTLKFLGEIDEATAEEVKKSLEEIAKKHKKHRVRVRGIGVFPNPNYVRVIWAGIENDEGIKAIAADVEREMRRLGFKKEKDFVAHITIGRVKFVKDKLELAMALKDLANEEFGEFEVNAIELKKSTLTPKGPIYETVARFELAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6F5</accession>
    <name>A0A218P6F5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853628.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6F5"/>
    <dbReference type="GeneID" id="33315200"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02970"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058357">
        <property type="entry name" value="DUF8044"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26161">
        <property type="entry name" value="DUF8044"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="79" mass="8795" checksum="60B12A882FD05A11" modified="2017-09-27" version="1">MAMRENVILFLAVWIVVSAILSPSTGIFLTIALIGILIALEVGEFYLTKDVKDSLKFSAYLLLLAFAFIVARKVYEVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6H9</accession>
    <name>A0A218P6H9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Xanthine phosphoribosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_02940</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853623.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6H9"/>
    <dbReference type="GeneID" id="33315194"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02940"/>
    <dbReference type="OrthoDB" id="4952at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363">
        <property type="entry name" value="HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363:SF1">
        <property type="entry name" value="HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="3">
        <location>
            <begin position="14"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="154" mass="17589" checksum="DCDA7961EC3AC732" modified="2017-09-27" version="1">MDKVYLTWWQVDRAIFALADELRKNFMPDVIVGVARGGLIPAVRLSHILGDVEVKVIDVKFYKGIDERMEKPVITIPLHGEFKGKKVVIVDDVSDTGKTLEVVIDEVKKAGASEVKVVCLSMKPWTKVVPDFYVFRTDKWVVFPWEEFPVVVRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6L9</accession>
    <name>A0A218P6L9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tubulin-like protein CetZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cetZ</name>
        <name evidence="8" type="ORF">A3L08_03340</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in cell shape control.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the CetZ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853690.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6L9"/>
    <dbReference type="GeneID" id="33315274"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03340"/>
    <dbReference type="OrthoDB" id="329751at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000065">
        <property type="entry name" value="Tubulin-like protein CetZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01946">
        <property type="entry name" value="CetZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032907">
        <property type="entry name" value="CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048737">
        <property type="entry name" value="CetZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045061">
        <property type="entry name" value="FtsZ/CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314">
        <property type="entry name" value="CELL DIVISION PROTEIN FTSZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314:SF10">
        <property type="entry name" value="TUBULIN-LIKE PROTEIN CETZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21011">
        <property type="entry name" value="CetZ_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00423">
        <property type="entry name" value="CELLDVISFTSZ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 6" id="KW-0133">Cell shape</keyword>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="7">
        <location>
            <begin position="2"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="10"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="103"/>
            <end position="105"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="389" mass="42491" checksum="922025CE843A170D" modified="2017-09-27" version="1">MRALIIGVGQCGTKIADLFSLVDFEALAVNTSKGDLEYLKHIPHERRILIGEGLTRGKGVNANPILGREAMKRDLPLVMRKIGSIIGYEDIDIFFLTFGFGGGTGAGGTPILAEALKEEYPDSLVVAIGALPLKEEGIRPTINAAITIDKLSKIADSIIAIDNNKLKEGDDDISKAYEKINYTIVERIASLLALVDVPGEQTLDSSDLKFVLKAFGSFATVGYAKADAGKVKSLSRLIMKSFESEGLYLEANIESALYGLVAVHGPPEVLKASDIFEALDYLTGKIRGKQIFRGFYPDPREREVEVVTLLSGIYDSRSIEDIIITAKNYAQSFMKAREEAETKKKKLLSGLPDFDDVYPPGETGVSELYDGSFPDVDGITKKLRRQEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6M4</accession>
    <name>A0A218P6M4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pyridoxal 5'-phosphate synthase subunit PdxS</fullName>
            <shortName evidence="7">PLP synthase subunit PdxS</shortName>
            <ecNumber evidence="7">4.3.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pdx1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pdxS</name>
        <name evidence="11" type="ORF">A3L08_03215</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of pyridoxal 5'-phosphate from ribose 5-phosphate (RBP), glyceraldehyde 3-phosphate (G3P) and ammonia. The ammonia is provided by the PdxT subunit. Can also use ribulose 5-phosphate and dihydroxyacetone phosphate as substrates, resulting from enzyme-catalyzed isomerization of RBP and G3P, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + phosphate + 3 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">In the presence of PdxT, forms a dodecamer of heterodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">Belongs to the PdxS/SNZ family.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="7"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853668.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6M4"/>
    <dbReference type="GeneID" id="33315249"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03215"/>
    <dbReference type="OrthoDB" id="6840at2157"/>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04727">
        <property type="entry name" value="pdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000406">
        <property type="entry name" value="Pyridoxal 5'-phosphate synthase subunit PdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01824">
        <property type="entry name" value="PdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001852">
        <property type="entry name" value="PdxS/SNZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033755">
        <property type="entry name" value="PdxS/SNZ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033983">
        <property type="entry name" value="Thiazole_synthase_ThiG"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003215">
        <property type="entry name" value="PRK04180.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00343">
        <property type="entry name" value="pyridoxal 5'-phosphate synthase lyase subunit PdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829:SF0">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01680">
        <property type="entry name" value="SOR_SNZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05690">
        <property type="entry name" value="ThiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029271">
        <property type="entry name" value="Pdx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01235">
        <property type="entry name" value="PDXS_SNZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51129">
        <property type="entry name" value="PDXS_SNZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0704">Schiff base</keyword>
    <feature type="domain" description="PdxS/SNZ N-terminal" evidence="9">
        <location>
            <begin position="13"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Thiazole synthase ThiG" evidence="10">
        <location>
            <begin position="247"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with D-ribose 5-phosphate" evidence="7">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="278"/>
            <end position="279"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="335" mass="36988" checksum="BAE39F5A6178DE00" modified="2017-09-27" version="1">MGKLDIIEAKGTERLKRGFAKMVKGGVIMDVTNAEQARIAEEAGAVSVMALHRVPADIRKAGGVARMAPIEKIQEIMDAVTIPVMAKVRIGHVAEARILEALGVDMIDESEVLTPSDPYFHIDKREFKVPFVCGNRNLGEAVRRIWEGAAMMRTKGEAGTGNIVEAVRHVRLLKDNIALIQRMTDEQVYGVAEKFAEPYLRLAFEVREISGLPRQVLENEPVYGHYTYREIVEGLYKILLEIKKLGRLPVVNFAAGGVATPADAALMMQMGMDGVFVGSGIFKSSNPGKMARAIVEAVNHWDEPDVLVEISKEIGEPMRGQDIEELEVRLEERGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6M8</accession>
    <name>A0A218P6M8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Squalene cyclase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03345</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853691.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6M8"/>
    <dbReference type="GeneID" id="33315275"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03345"/>
    <dbReference type="OrthoDB" id="86043at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00688">
        <property type="entry name" value="ISOPREN_C2_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="736" mass="82842" checksum="146C4E9C5FD1E6F4" modified="2017-09-27" version="1">MKKVLAVIIIVLMLVPAVSAEAIDGSVRFLSGTSENTQQVREISLSIMALAAAKDDVNWDITPEIEVLVNELLEDQNADGGWGYYPNEPSNVLDTAYAVIALRTFIDNVSSPLHAEASVSLKKGLRYLVSANNGEAWGYIPKTTSECYPTLIALWALGMNKYTIHREIVKNAIEYLSSVETCEISPHERFALELIAYHYLKVPVNSEQIERAKYILLNETLTTKERAVLTYALTLYEPVDFNTLRALHILINQGQKTIDNLTYWTNVPNCFGTSELVATTAYAIMGISRSYQKPIPPESFNETTMDLCSVLASAQNPDGGWPFSDFQESNPTVTYYALKALSSCYPRDSKIIQNALTFMRKVMKKEEDKIKLRNYISPEYYYAVKALAESGELSSNEKNEIIRNILNSRLQGTNLWGSKILGPQPLDTAYAIDLLLDLGISPSDPVIQSATKWLMDISSTGWGTYMGGRYIKYMLKPNLFVTMVVLKTLTRVVNEKEVREHIDWLESQRWDKGWGYYKYYEDITGRRIPQEPEIFITTEITTLLLEYGIDYREDTLTLILENLNDIKENYLDLAASIAFIAKIRPLVTLENIVSILNTQSFKVFADSDEYNISKIASNLHSVFGQQFIKTKTISKEGNYVIISKIGGIDVQEYNPQITVEILNSSIKIGDIEVPKSDVVLIVPGRTSNGFALLILYTPGTEEIVKEIFSIGFIKYIRGDAMVLVNENGKARVIVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6P0</accession>
    <name>A0A218P6P0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853713.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6P0"/>
    <dbReference type="GeneID" id="33315300"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03475"/>
    <dbReference type="OrthoDB" id="382387at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="230" mass="25688" checksum="4FB39CF7923CDD13" modified="2017-09-27" version="1">MPLRIVDHPVVLMPFLLLAVSLLTGEKVSWILMGLSLWLIVYISFTGEAKIEGQKIVIRIGRPIPLAKKEILLDEVVEVIELPGAYGIRMIGQFQRPWVPIGCLTTGLFVGILLLLRGESYGIFWMYISLLSSLDYLFRPVDKRIRVVASGIISLFSALAFFYLRHPEFSLGVIAYGFFDVISSEDYGQDAIIIKTENEKIVLLGESASKERFLKGLRSIIVGGLNAQTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6P5</accession>
    <name>A0A218P6P5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidase M28</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_03445</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853708.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6P5"/>
    <dbReference type="GeneID" id="33315295"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03445"/>
    <dbReference type="OrthoDB" id="18376at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045175">
        <property type="entry name" value="M28_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147">
        <property type="entry name" value="METALLOPEPTIDASE M28 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147:SF26">
        <property type="entry name" value="PEPTIDASE M28 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PA" evidence="1">
        <location>
            <begin position="117"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="2">
        <location>
            <begin position="228"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="562" mass="63598" checksum="D76D69889A88BC68" modified="2017-09-27" version="1">MRRFLKEAEVFDPNRVLHYIAEISQFHRIQGSKELPEAVRFIREELRIWGISPELYEEVYDGESRYLTLRAPIAWDVVEGKVEVLGKTITTGQSPLVVMAHSPSGSAEGEIVHVAREKDWENVEGKIVLAGKEWREAYRRANEKGAAGFIAYREGTGNAIPYIGLFLTKADLEWARIPAVAVPEGLAKEMIRKLNSGEKVEAKIEVKAQIKEREVLPILYAEIGEPPFILFTAHICHPKPGANDNASGSAMLMELARVLSRLHDGSSRFGFAFLWVPEYYGTQAFVEQYAELEKYYAVINLDMVAGSPDRSGSTVMLVRTPLSRFSIVSGLLEYFLELANEGGKSFSGSPLPRLRLKSFPYEMGSDHDVFNFFGIPSTMPITWPDRFYHSSEDTVDKVSKESIEIIGKAVLATALALAKAEGDELRRFARGYAMKYLGELSREQETEEAERLVMMGLARDSDFLGIESGHRFEEKPWLVWEIKGVVSERFIRERDEKLAEEFKELTGDRKVLAHLHELLMLGELLPKERAFEALKEEYGDVEGEKLERLVIILEKAGVVRFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Q3</accession>
    <name>A0A218P6Q3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0145 protein A3L08_03500</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_03500</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0145 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853717.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Q3"/>
    <dbReference type="GeneID" id="33315305"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03500"/>
    <dbReference type="OrthoDB" id="59443at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.70">
        <property type="entry name" value="Hypothetical protein apc22750. Chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00338">
        <property type="entry name" value="UPF0145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035439">
        <property type="entry name" value="UPF0145_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002765">
        <property type="entry name" value="UPF0145_YbjQ-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002989">
        <property type="entry name" value="PRK03732.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34068:SF2">
        <property type="entry name" value="UPF0145 PROTEIN SCO3412"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34068">
        <property type="entry name" value="UPF0145 PROTEIN YBJQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01906">
        <property type="entry name" value="YbjQ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117782">
        <property type="entry name" value="YbjQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="112" mass="12167" checksum="31B96AEF987F56A6" modified="2017-09-27" version="1">METIEDVIVVTTEAIPGYRIVEVKGIARGGIVKATHLGRDIMAVLRNIKGGEVKEYTEMMAEAREEALRRMVLHAKEMGANAVVDVRFATSNVGSSVAEVYAYGTAVVVEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6R3</accession>
    <name>A0A218P6R3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">SMC-Scp complex subunit ScpB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_03630</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853740.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6R3"/>
    <dbReference type="GeneID" id="33315331"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03630"/>
    <dbReference type="OrthoDB" id="8628at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051304">
        <property type="term" value="P:chromosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005234">
        <property type="entry name" value="ScpB_csome_segregation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00281">
        <property type="entry name" value="SMC-Scp complex subunit ScpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34298">
        <property type="entry name" value="SEGREGATION AND CONDENSATION PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34298:SF2">
        <property type="entry name" value="SEGREGATION AND CONDENSATION PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04079">
        <property type="entry name" value="SMC_ScpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019345">
        <property type="entry name" value="ScpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="179"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="209" mass="23555" checksum="F3D022D4990F2768" modified="2017-09-27" version="1">MGLLEDKALVEAALFVSGRPLSIKELSRALGIRSLDYLEKLIELIAAEYAERKSAIEVVKVLGDKYVMQVKQEYSQRVVHLMPRPDLRTGELKTLALIAYLQPIEQSKVVKLRGSQAYEHIRKLLEMGLIYAEPYERTKLLGTTSKFAELYGFPENDPNIIKEAFKRVVHAEYSDLISKLEGKGEDEEAPEGEGMKEVESTEAISPAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6S2</accession>
    <name>A0A218P6S2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_03665</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853744.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6S2"/>
    <dbReference type="GeneID" id="33315338"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03665"/>
    <dbReference type="OrthoDB" id="86208at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43357">
        <property type="entry name" value="INNER MEMBRANE ABC TRANSPORTER PERMEASE PROTEIN YDCV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43357:SF4">
        <property type="entry name" value="INNER MEMBRANE ABC TRANSPORTER PERMEASE PROTEIN YDCV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="9"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="54"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="191"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="289"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="351"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="383"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="409"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="463"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="512"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="56"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="345"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="541" mass="60623" checksum="6F1790F0E2227720" modified="2017-09-27" version="1">MRIRPTQLVLLIPLAFLMVFFYIPLVSIIYMGLWDNGPTLRYISSVLANDYHRRVILFTVGQAIASTLLTLAFGLPGAYIFAKYDFPGKRAVKAVLTVPFVMPSVMVALGYILLFGKNGFVTGLIGRDLGILYSWKGILLAHAFYNFPIVIRMVSSLWQRINPHYEEAAMALGARGWKLFWRVTLPMLSPAIFASAMLTFVFCFLSFSIPLIIGGYQYATIEVDIFTSIMTLLDFKTGSALAIIQITLSVVFMYIYLRSLDAYAKREEQRVFRKPVPFTKRDWLSLRGLAIAVYSFIVFIFIVSPLLAVIYDSLHFNGQWSLEWYRRVFSAEYNPMFGATTLGAVRNSLTFGLATVFLSVLVALPIAYALHRWNFRGKRLFDVLAMLPLASSAITLGLGYIRVFHATPLYYTMWIVIAAHTVIAYPFVLRAVSTSLKKIRPNLFEAALSLGAREWKAFLKVELPLAIGGVIVGAIFAFAMSIAELGATYMLAKPEYTTMAVAIYKFLGARQFGSASALSVLLMAISTLSFLVIERIGEEVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6S6</accession>
    <name>A0A218P6S6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Guanine permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_03655</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. Azg-like subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853742.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6S6"/>
    <dbReference type="GeneID" id="33315336"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03655"/>
    <dbReference type="OrthoDB" id="27788at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005345">
        <property type="term" value="F:purine nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045018">
        <property type="entry name" value="Azg-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026033">
        <property type="entry name" value="Azg-like_bact_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43337">
        <property type="entry name" value="XANTHINE/URACIL PERMEASE C887.17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43337:SF1">
        <property type="entry name" value="XANTHINE_URACIL PERMEASE C887.17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005353">
        <property type="entry name" value="PbuG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="336"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="387"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="425"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR005353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="445" mass="47186" checksum="22B16E76A53F8628" modified="2017-09-27" version="1">MGWFEDYFEFDKHRTDMKTEILAGVTTFMTMAYILFVNPSILSDAMGKEAFNSLVAVTALAAGFATILMGLYAKKPFALAPGMGLNAYFAYSVVLGMGYDWRIALAAVFVEGLIFIALSVTKVRSAIIHAIPLSQKYAVGAGIGLFLVFIGLNDVGLLTAVVNDSGVLQFTGLNTGALASKEIVLFFFGLFLAAILISLRIKGALLISIITTSVLGWLTGAAPWPDHLFSTPDISYTFLKMDLKGLLNVGALGVIFAFFMVDFFDTLGTVTGLSAKAGFLTKDGKVPDAEKVLLTDAIGTTVGAVLGTSTVTTYIESAAGIEEGGRTGMTALVTGLLFLGIGLFIAPLAGAIPAFATAPALVIVGYYMLSAVKEIDFTDHTEAIPAFLVLVMIPYTYSIADGIGAGFISYTLLKLFSGRGRELHPLMYALAIVFLAYFAYLGGAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Z8</accession>
    <name>A0A218P6Z8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADH dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_03995</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 51 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853805.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Z8"/>
    <dbReference type="GeneID" id="33315403"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03995"/>
    <dbReference type="OrthoDB" id="297477at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02980">
        <property type="entry name" value="TRX_Fd_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.230:FF:000001">
        <property type="entry name" value="Mitochondrial NADH dehydrogenase flavoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11540:FF:000001">
        <property type="entry name" value="NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11540">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 51kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.230">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 51kDa subunit, iron-sulphur binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011538">
        <property type="entry name" value="Nuo51_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037225">
        <property type="entry name" value="Nuo51_FMN-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019575">
        <property type="entry name" value="Nuop51_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037207">
        <property type="entry name" value="Nuop51_4Fe4S-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019554">
        <property type="entry name" value="Soluble_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010120">
        <property type="entry name" value="PRK13596.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43578">
        <property type="entry name" value="NADH-QUINONE OXIDOREDUCTASE SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43578:SF3">
        <property type="entry name" value="NADH-QUINONE OXIDOREDUCTASE SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01512">
        <property type="entry name" value="Complex1_51K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10589">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10531">
        <property type="entry name" value="SLBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00928">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142019">
        <property type="entry name" value="Nqo1 FMN-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142984">
        <property type="entry name" value="Nqo1 middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140490">
        <property type="entry name" value="Nqo1C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="538"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="568"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="600" mass="66029" checksum="0199F6F461B234AD" modified="2017-09-27" version="1">MSEIKAIAVGMNSCGIAAGAKETYEALKRELEERGLDVKLKIVGCVGMCYREPLVDIITDDEIITYGHVDPKKVPRIIEEHVINGKPIKEWIVKRDWWENGERKTWDVDGYFAKQKKIVLENSGYIDPENIDEYIAAGGYEALKKALQMEPEEIIDVIIKSGLRGRGGAGFPTGMKWKFTRQAPGDEKYIVCNADEGDPGAFMDRNVLEGDPHRVIEGMIIGAYAIGATKGFIYVRAEYPLAIRRLKIALKQARERGFLGENILGSGFSFDIAIKEGAGAFVCGEETALIASIEGKRGMPRPRPPYPAQKGLWGKPTNINNVETWANVPWIIKHGWQAYASLGTEKSKGTKVFALSGKIKHGGNVEVPMGITLREILYEIGGGTKTGKRIKAVQLGGPSGGCIPEELFDTPVDYESVNATGAIMGSGGMVVMDEDTCMVDVAKFFLDFTVKESCGKCTFCRLGTKRMWEILDKFTRGEATLEDLEKLEKLAYQVKAGSLCGLGQTAPNPVLTTLRYFRDEYLAHIEGKCPAKVCKPLIRYVIIADKCTGCTACAIFCPAKVISGERLKPHSIDQEACIKCGTCYEVCRFNAIEILTGRDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Z9</accession>
    <name>A0A218P6Z9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl24p</name>
        <name evidence="4" type="synonym">rpl24</name>
        <name evidence="6" type="ORF">A3L08_04105</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Located at the polypeptide exit tunnel on the outside of the subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="4">One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853826.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Z9"/>
    <dbReference type="GeneID" id="33315425"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04105"/>
    <dbReference type="OrthoDB" id="10899at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000009">
        <property type="entry name" value="60S ribosomal protein L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01326_A">
        <property type="entry name" value="Ribosomal_uL24_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005825">
        <property type="entry name" value="Ribosomal_uL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005756">
        <property type="entry name" value="Ribosomal_uL24_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="Ribosomal_uL24_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01080">
        <property type="entry name" value="rplX_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11143">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L26 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16906">
        <property type="entry name" value="Ribosomal_L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01108">
        <property type="entry name" value="RIBOSOMAL_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="KOW" evidence="5">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="121" mass="14071" checksum="D882F86C99145DD8" modified="2017-09-27" version="1">MKLDTKQPRKQRKFLHTAPLHLRSKIMAATLSPELRNKYGIRSIPIRVGDKVRVMRGDFKGKEGKVVELDLKRYRIHVEGVTHKKTDGTEVFYPLHPSNVMIIELNLDDEKREKIISRRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P716</accession>
    <name>A0A218P716_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable tRNA pseudouridine synthase B</fullName>
            <ecNumber evidence="5">5.4.99.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">tRNA pseudouridine(55) synthase</fullName>
            <shortName evidence="5">Psi55 synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">tRNA pseudouridylate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">tRNA-uridine isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">truB</name>
        <name evidence="8" type="ORF">A3L08_04200</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 5">Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>uridine(55) in tRNA = pseudouridine(55) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42532"/>
            <dbReference type="Rhea" id="RHEA-COMP:10101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10102"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the pseudouridine synthase TruB family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="5"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853844.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P716"/>
    <dbReference type="GeneID" id="33315444"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04200"/>
    <dbReference type="OrthoDB" id="35866at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160148">
        <property type="term" value="F:tRNA pseudouridine(55) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000495">
        <property type="term" value="P:box H/ACA sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031118">
        <property type="term" value="P:rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02572">
        <property type="entry name" value="PseudoU_synth_hDyskerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21148">
        <property type="entry name" value="PUA_Cbf5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.10:FF:000001">
        <property type="entry name" value="H/ACA ribonucleoprotein complex subunit CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.10:FF:000010">
        <property type="entry name" value="Probable tRNA pseudouridine synthase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01081">
        <property type="entry name" value="TruB_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012960">
        <property type="entry name" value="Dyskerin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004802">
        <property type="entry name" value="tRNA_PsdUridine_synth_B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026326">
        <property type="entry name" value="TruB_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00425">
        <property type="entry name" value="CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003280">
        <property type="entry name" value="PRK04270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127">
        <property type="entry name" value="CENTROMERE/MICROTUBULE BINDING PROTEIN CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127:SF0">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT DKC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08068">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01136">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Dyskerin-like" evidence="7">
        <location>
            <begin position="4"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="PUA" evidence="6">
        <location>
            <begin position="251"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00060072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00060775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="336" mass="38038" checksum="AAD8560EA0AA4A30" modified="2017-09-27" version="1">MARDEVRRILPADIKREVLIKDEKAETNPKWGFPPEKRPMEMHMQFGIINLDKPPGPTSHEVVAWVKRLFNLSKAGHGGTLDPKVSGILPVALERATRVVQALLPAGKEYVALMHLHGDVPEDRIRAVMKEFEGEIIQRPPLRSAVKRRLRTRKVYYIEVLEIDGRDVLFRVGVEAGTYIRSLIHHFGLALGVGAHMAELRRTRSGPFKEDETLVTLHDLVDYYHFWKEDGIEEYFRKAIQPMEKAVEHLPKVWIRDSAVAAVTHGADLAVPGIVKLNKGIKKGDLVAIMTLKDELVALGKATMTSGEMLQKSKGIAVDVDKVFMPREWYPKLWAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P718</accession>
    <name>A0A218P718_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rps14P</name>
        <name evidence="7" type="synonym">rps14</name>
        <name evidence="8" type="ORF">A3L08_04120</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds 16S rRNA, required for the assembly of 30S particles.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the universal ribosomal protein uS14 family. Zinc-binding uS14 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853829.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P718"/>
    <dbReference type="GeneID" id="33315428"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04120"/>
    <dbReference type="OrthoDB" id="5615at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.830.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.10">
        <property type="entry name" value="30s Ribosomal Protein S14, Chain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01364_A">
        <property type="entry name" value="Ribosomal_uS14_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001209">
        <property type="entry name" value="Ribosomal_uS14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023676">
        <property type="entry name" value="Ribosomal_uS14_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018271">
        <property type="entry name" value="Ribosomal_uS14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039744">
        <property type="entry name" value="RIbosomal_uS14_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043140">
        <property type="entry name" value="Ribosomal_uS14_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004424">
        <property type="entry name" value="PRK05766.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12010">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12010:SF2">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00253">
        <property type="entry name" value="Ribosomal_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00527">
        <property type="entry name" value="RIBOSOMAL_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="56" mass="6584" checksum="B5D96B63CCD1F686" modified="2017-09-27" version="1">MAKADYNKRKPRKFGKGARRCVRCGQYGPVIRIHGLMLCRHCFREIAPKLGFKKYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P722</accession>
    <name>A0A218P722_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Large ribosomal subunit protein eL14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpl14e</name>
        <name evidence="5" type="ORF">A3L08_04190</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853842.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P722"/>
    <dbReference type="GeneID" id="33315442"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04190"/>
    <dbReference type="OrthoDB" id="63594at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06088">
        <property type="entry name" value="KOW_RPL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000045">
        <property type="entry name" value="50S ribosomal protein L14e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00721">
        <property type="entry name" value="Ribosomal_eL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039660">
        <property type="entry name" value="Ribosomal_eL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023651">
        <property type="entry name" value="Ribosomal_eL14_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041985">
        <property type="entry name" value="Ribosomal_eL14_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003320">
        <property type="entry name" value="PRK04333.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="KOW" evidence="4">
        <location>
            <begin position="6"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="83" mass="9014" checksum="4A352E95E2725296" modified="2017-09-27" version="1">MPAMEVGRLAVVIAGRRAGQKVVVVDVIDRNFVLVTGAGLNKVKRRRMNVKHLEPLPEKVNIERGADDEAVKAALEQAGISLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P729</accession>
    <name>A0A218P729_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rps4</name>
        <name evidence="11" type="ORF">A3L08_04220</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="7">With S5 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">Part of the 30S ribosomal subunit. Contacts protein S5. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853846.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P729"/>
    <dbReference type="GeneID" id="33315448"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04220"/>
    <dbReference type="OrthoDB" id="10429at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000026">
        <property type="entry name" value="30S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01306_A">
        <property type="entry name" value="Ribosomal_uS4_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022802">
        <property type="entry name" value="Ribosomal_uS4_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_uS4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005710">
        <property type="entry name" value="Ribosomal_uS4_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003139">
        <property type="entry name" value="PRK04051.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01018">
        <property type="entry name" value="uS4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF5">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7 11" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 7 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7 8" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS4 N-terminal" evidence="10">
        <location>
            <begin position="5"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="RNA-binding S4" evidence="9">
        <location>
            <begin position="103"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="180" mass="21005" checksum="7C66B6A4A28EF3FC" modified="2017-09-27" version="1">MGDPKRQRKRYETPSHPWIKERLDRERVLMKKYALKNKKELWRHETQLKEFRRRARRLLAARGKQAEIEKVQLLQRLNRLGLLPADAVLDDVLSLTVEDVLDRRLQTVVFKKGLARTIGQARQLIVHGHIEVNGQVIRSPGYLVLKEEEDTITYAKNSPFAKESHPERMVIEQAKQGGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P736</accession>
    <name>A0A218P736_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA-directed RNA polymerase subunit Rpo10</fullName>
            <ecNumber evidence="9">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">DNA-directed RNA polymerase subunit N</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rpo10</name>
        <name evidence="9" type="synonym">rpoN</name>
        <name evidence="10" type="ORF">A3L08_04255</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the archaeal Rpo10/eukaryotic RPB10 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="9"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853850.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P736"/>
    <dbReference type="GeneID" id="33315455"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04255"/>
    <dbReference type="OrthoDB" id="371754at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000335">
        <property type="entry name" value="DNA-directed RNA polymerase subunit N, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00250">
        <property type="entry name" value="RNApol_arch_Rpo10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023580">
        <property type="entry name" value="RNA_pol_su_RPB10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020789">
        <property type="entry name" value="RNA_pol_suN_Zn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000268">
        <property type="entry name" value="RPABC5/Rpb10"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003089">
        <property type="entry name" value="PRK04016.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431:SF3">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01194">
        <property type="entry name" value="RNA_pol_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005653">
        <property type="entry name" value="RNA_pol_N/8_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46924">
        <property type="entry name" value="RNA polymerase subunit RPB10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01112">
        <property type="entry name" value="RNA_POL_N_8KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 9 10" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="65" mass="7706" checksum="F0E205873753519F" modified="2017-09-27" version="1">MIVPVRCFTCGKVIGDKYYEFKERVEKGEDPEKVLDDLGVERYCCRRTLLSHVELIDQVMVYKVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P747</accession>
    <name>A0A218P747_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Large ribosomal subunit protein uL5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rpl5</name>
        <name evidence="12" type="ORF">A3L08_04115</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">This is 1 of the proteins that bind and probably mediate the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. May contact the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7 8">Part of the 50S ribosomal subunit; contacts the 5S rRNA and probably tRNA. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853828.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P747"/>
    <dbReference type="GeneID" id="33315427"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04115"/>
    <dbReference type="OrthoDB" id="372044at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1440.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01333_A">
        <property type="entry name" value="Ribosomal_uL5_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_uL5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022804">
        <property type="entry name" value="Ribosomal_uL5_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_uL5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057266">
        <property type="entry name" value="Ribosomal_uL5_euk/arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_uL5_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003258">
        <property type="entry name" value="PRK04219.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 8 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 8" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="2 8" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL5 N-terminal" evidence="10">
        <location>
            <begin position="15"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL5 C-terminal" evidence="11">
        <location>
            <begin position="72"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00065757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="183" mass="20854" checksum="9D1C544F346C582F" modified="2017-09-27" version="1">MQINREAILADWEAHPMRKPRIAKVTINIGVGESGERLTKAETMLEGLVGQKPIRRRAKQTNRDFGIRRGEPIAVKVTLRGEKAYQMLDRLLEAVDRKLNVSNFDEHGNFCFGIQEHINIPGVEYDPEIGIFGMDVCVTLERPGFRVAKRKRQRKKLPTKHKLAKEEGIVFAIEELKAKVEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P784</accession>
    <name>A0A218P784_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Threonine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_04545</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853898.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P784"/>
    <dbReference type="GeneID" id="33315513"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04545"/>
    <dbReference type="OrthoDB" id="6371at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01563">
        <property type="entry name" value="Thr-synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004170">
        <property type="entry name" value="PRK05638.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF6">
        <property type="entry name" value="L-THREONINE DEHYDRATASE CATABOLIC TDCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="4">
        <location>
            <begin position="365"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="443" mass="48893" checksum="E97F10F4E1B77F6A" modified="2017-09-27" version="1">MKVRCPGCGRLYSSIIPPTCCGEPLRIEYDYEGVDISRWSNREPGVWRYRELLPEVPEVVSLREGGTPVIRARLGEELGYSVFIKDETRNPTGSFRDRLVTVAVSYGLPHAENGFVVASNGNAAASLAAYAARTGKPAYTVVPKLIEQGKLNQIAAFGARLIRYGESVDEDISYAEGLAEGKGLYNITPESNLIGLEGQKTIAFELWEKVRPTHVIVPTGSGSNLYSIYKGFAELMKIGVLDEMPKLIAVQTERCSPIASEVLGVEPRVEPTKALGLYVKNPVMKELALEAINGSGGTAVLVGEDELDLGQRLLAKEGIFAEYASSVIVPALLKLAEEGYFERDDRIALIVTSSGLKGHYSESREKFSVGGTKLDILKLLSEREMYGYEIWEALEKPLKYQAVYQHLRELESMGLIEETHRKGRRVYYGLTEKGRKFLETLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P790</accession>
    <name>A0A218P790_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA cytosine methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_04590</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P790"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04590"/>
    <dbReference type="OrthoDB" id="86126at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05753">
        <property type="entry name" value="TRAP_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="626"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="658"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="670"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="687" mass="76071" checksum="2817B58C9FFD01B6" modified="2017-09-27" version="1">MRKPALILASLLILSLFSSWSVFAENQVKYAAINDVSLGLKDQVKIGPYSVKFSDADPDWKRVSFEVSGPSGSKIFVGTDGDVFYYPSEGDQYLAIRLVWVSEASGKVVISIKAPLREIMTTENPLKKGDHVSLPAGFPDIQIYVDSISTESKKVTFEISYPYGDTEHLTLQEGEGDGIDYRLPGGYIYSNYVYIDVLSISKDGVKFTVYAPRVASTDFKIIRNNENGNQPSETPEQTVLVYNGLLYVDENLPVKVDNTTYYVKVVSTIPDVAKVEILKGSQSLGTMLLEVGDVPKNVPNTPLQLSVQGVEPDYQRATIRVYAPMGAQVTPILRQANLVAKIDAIPKEIMLDDNLVITINVQNLGRGDAYDVNVAAPIPDDFELVSMAESWTLKNLPAFTNMPALIYVLKPTKVGEFDIGKVIVTFYDDKSLDTGKKRVVYSQPLTGIKVYSIPTLDVTAQAYNGTWGNYVSAKVNDTVKLKFTVRASDGNPDYEFIKNATLLLDLTSNLDGQPVIDVGMLKAGETKVVSLDVKVLKENLTNVRATLIYLDPLGGQHRLDLGNIVTINSIPPKVIVEEVKVWPTPDELPDYVNKTLSGMEDPMPLASELANISTYYLPKEEKSNPWQPTAIIFIILTIVLAGMAYKYWDEAQKLREKLERKKQHRPGGLPKKEGEKESKESSEIVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P796</accession>
    <name>A0A218P796_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3-hexulose-6-phosphate synthase</fullName>
            <ecNumber evidence="4">4.1.2.43</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_04625</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-ribulose 5-phosphate + formaldehyde = D-arabino-hex-3-ulose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:25201"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58542"/>
            <dbReference type="EC" id="4.1.2.43"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HPS/KGPDC family. HPS subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SIS family. PHI subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.43" evidence="4"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853912.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P796"/>
    <dbReference type="GeneID" id="33315529"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04625"/>
    <dbReference type="OrthoDB" id="350569at2157"/>
    <dbReference type="UniPathway" id="UPA00294">
        <property type="reaction ID" value="UER00434"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043801">
        <property type="term" value="F:hexulose-6-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019647">
        <property type="term" value="P:formaldehyde assimilation via ribulose monophosphate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04726">
        <property type="entry name" value="KGPDC_HPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05005">
        <property type="entry name" value="SIS_PHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000022">
        <property type="entry name" value="3-keto-L-gulonate-6-phosphate decarboxylase UlaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017553">
        <property type="entry name" value="3-hexulose-6-phosphate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041710">
        <property type="entry name" value="HPS/KGPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017552">
        <property type="entry name" value="PHI/rmpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03128">
        <property type="entry name" value="RuMP_HxlA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041755">
        <property type="entry name" value="RuMP_HxlAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03127">
        <property type="entry name" value="RuMP_HxlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43443">
        <property type="entry name" value="3-HEXULOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43443:SF1">
        <property type="entry name" value="3-HEXULOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SIS" evidence="7">
        <location>
            <begin position="241"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="406" mass="44261" checksum="5612A9546154E3B2" modified="2017-09-27" version="1">MILQVALDLTDIEQAISIAEKAARGGAHWLEVGTPLIKKEGMRAVELMKRRFPDRKIVADLKTMDTGALEVEMAARHGADVVSILGVADDKTIMDAVEVARRYGIRVMVDLIGVKDKVKRAKELEKMGVHYILVHTGIDEQVQGKSPLEDLEKVVKAVKVPVAVAGGLNLQTIPKVVELGATIIIVGGAITKAEDPEDVTRKIIDLFWGEYMMTIRKAMTDITEHINGVAESLKLEQVRGFVDAMIGANKIFIYGAGRSGLVGKAFAMRLMHLDFNVYVVGETITPAFEQGDLLIAISGSGETKSIVDAAQIARAQGGKVVAITSYANSTLGKLADVVLEIPGRTKSDVPTDYIARQMLTKYKWIAPMGTLFEDSTMIFLDGVIALLMATFQKTEKDMKRKHATLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P799</accession>
    <name>A0A218P799_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcription elongation factor NusA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_04600</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853907.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P799"/>
    <dbReference type="GeneID" id="33315524"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04600"/>
    <dbReference type="OrthoDB" id="4111at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005013">
        <property type="entry name" value="PRK06418.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="57"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="170" mass="18643" checksum="07BA1DDE869C3587" modified="2017-09-27" version="1">MKAPICEVCLKTDDILCPADEKKLQEGLISELDVKVARLLYKLLGDVDIEFKKAVEAGDIIVIMVGEGDVPVTIGKGGKNIKALMRELGKRIRVIEAVEVKDTEDLKKLATDLLYPAGVFGVNIVYKPGGGNYYKVLVLSRDRKKLPEKADVLESILSQMAGAEVKINFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7C2</accession>
    <name>A0A218P7C2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutaredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_04735</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853933.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7C2"/>
    <dbReference type="GeneID" id="33315551"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04735"/>
    <dbReference type="OrthoDB" id="35385at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02975">
        <property type="entry name" value="PfPDO_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02973">
        <property type="entry name" value="TRX_GRX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011903">
        <property type="entry name" value="TON_0319-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02187">
        <property type="entry name" value="PDO_seleno_TRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37170:SF1">
        <property type="entry name" value="GLUTAREDOXIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37170">
        <property type="entry name" value="GLUTAREDOXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13192">
        <property type="entry name" value="Thioredoxin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin-like fold" evidence="3">
        <location>
            <begin position="137"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="226" mass="25510" checksum="14C152AC3D335F50" modified="2017-09-27" version="1">MGLISDADKKVIKEEFFSKLTNPVKIIGFTGREHCQYCDQLKQLVQELSELTDKLTYEFHDFDTPEGKEVAEKYRIDRAPAVTITQDGKDMGVRYFGLPAGHEFGSFLEDIVDVSNATTDLMPESKEELAKIDRDVRILVFVTPTCPYCPLAVRMAHKFAIENTNAGKGKILGDMVEAIEYPEWADQYSVMAVPKIVIQVDGEDKVQFEGAYPEKMFLEKLLAALE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7F2</accession>
    <name>A0A218P7F2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Phospho-sugar mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_04895</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853958.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7F2"/>
    <dbReference type="GeneID" id="33315583"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04895"/>
    <dbReference type="OrthoDB" id="84553at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016868">
        <property type="term" value="F:intramolecular phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43771">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43771:SF1">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="7">
        <location>
            <begin position="9"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="235" mass="26662" checksum="ACEECF2439266D5E" modified="2017-09-27" version="1">MEVYHSPRFNPEELALLGRAIGTISHGTIIVGRDGRAISRYGKRAMVVGIVSTGSTIMDVRLIPLIALKDFAHRKGLPLAYVYYHNGVRVYVSGIDSDEIGAILESRSFIEAHPNDIGATVYYPNALDDFLHEVFKHYNFQVGGKVLVDAMNTPAVLFFPRMSDHFGFEVELINDMMTSYLPPKPKEVFLHKLSKGDYDFGLRFRPEGVVEFYREGEELEFGSMWKLLDHMKKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7G3</accession>
    <name>A0A218P7G3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Molybdopterin oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_04960</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853967.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7G3"/>
    <dbReference type="GeneID" id="33315596"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04960"/>
    <dbReference type="OrthoDB" id="84999at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.90">
        <property type="entry name" value="ADC-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.530.10">
        <property type="entry name" value="CPE0013-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036593">
        <property type="entry name" value="CPE0013-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012460">
        <property type="entry name" value="DUF1667"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39450:SF1">
        <property type="entry name" value="DUF1667 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39450">
        <property type="entry name" value="MOLYBDOPTERIN OXIDOREDUCTASE, 4FE-4S CLUSTER-BINDING SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07892">
        <property type="entry name" value="DUF1667"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04879">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160148">
        <property type="entry name" value="CPE0013-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53706">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase, domains 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="4">
        <location>
            <begin position="2"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="115" mass="12370" checksum="853F5AD870DA359D" modified="2017-09-27" version="1">MTTYRFTCIVCPLGCAIEVDVEDGKVLEVKGHTCPRGKEWAVQEVTNPKRVVMSVVPVEGGALPTVSVKTAEPVPKEKIPELMQFLAGIKVKAPVKIGDVVAEWEGIKIVATRGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7H2</accession>
    <name>A0A218P7H2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7H2"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05110"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="297" mass="33302" checksum="AD08D4789579603F" modified="2017-09-27" version="1">MNVKKYLLVVTVFSLILLIIPKGILVPTNAKGASFKEDVSSLECPFKIQTSLNFENFSYYFTIFTQDIQKSNYTLPGINGGYICIPEGVLLYAYFIQDSQSTLLFLGDNLTEKWKKEFPAYPLKHLNESLILVGNPRFGSSDTSCVYVIDISTGLLKNWFCPNVLGGYISDVKILHDRVYVTIGVPEAPPLRTRAFIYVKEGNKLRKSEIASISGEGVGIRLPIDANEKYVAVAYFLANERGEEKNGVCVLTARSLRKIACKELNEGERPLKVKVKDNIVYVQTTKGVKAYKILSLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7J3</accession>
    <name>A0A218P7J3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Arsenical-resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_05250</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the arsenical resistance-3 (ACR3) (TC 2.A.59) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854015.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7J3"/>
    <dbReference type="GeneID" id="33315654"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05250"/>
    <dbReference type="OrthoDB" id="326456at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015104">
        <property type="term" value="F:antimonite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015105">
        <property type="term" value="F:arsenite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1530.20:FF:000020">
        <property type="entry name" value="Arsenical-resistance membrane protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004706">
        <property type="entry name" value="Arsenical-R_Acr3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002657">
        <property type="entry name" value="BilAc:Na_symport/Acr3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00832">
        <property type="entry name" value="acr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43057:SF1">
        <property type="entry name" value="ARSENICAL-RESISTANCE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43057">
        <property type="entry name" value="ARSENITE EFFLUX TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01758">
        <property type="entry name" value="SBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005508">
        <property type="entry name" value="Acr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="84"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="228"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="258"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="289"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="319"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="364" mass="39806" checksum="DBFFF3C6D4AE151F" modified="2017-09-27" version="1">MGKKGLSLFEKYLSIWVALCIMAGIAIGKLFPALPETLSRLTIANVNMPIAVLIWAMIYPMMVKVDFSAIKRVHKGQMLKGLTVTWVTNWLIKPFSMFLISSFFIGALFSAKLGLIEPSLAKEYIAGAILLGAAPCTAMVFVWSYLADGDPLYTLVQVATNDLIILFAFAPIVGFLMGLNEVPVPYDTLLLSVVLFVVIPLTAGYLSRRHLLRTKGVEWFERNFLPKLSTVSIVGLLLTLILLFSFQGKIILENPLHIALIAVPLTIQTYFIFAIAYGWAWLWKLPHKVAAPASFIGASNFFELAVAVAIALFGLESGAALATVVGVLEEVPIMLSLVWIANKTRHLFTAKFEAGSAAPALAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7M0</accession>
    <name>A0A218P7M0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854038.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7M0"/>
    <dbReference type="GeneID" id="33315678"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05370"/>
    <dbReference type="OrthoDB" id="85161at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="299" mass="32904" checksum="645ABE775B2817EF" modified="2017-09-27" version="1">MRYSSIAVRLFEREGEVVFYDPAYHGRTLKVFGMDEWPDKALEHLAGKYMEKDYSRVIFDTKGSFSEEGFDTVLRIQDTKPSGLDPIKLAAEGHFDFYTAATIIQTIYGLDRTLTEMLYSDILAGKVGSVPEALKAGQKYSEVIAESYTALDQLLYSGEVPELGQNILVDFGDAHSITLVGNAFLILSAAVEKRRRVMVGLNDAAVLAYTTAGGAGLPILAKPALKRVTVVTSEYALDSLLNMSGPVLLLYHDPDVQSLIYEASGVPPGPMRKHVHKGQGAFIYRTPETIDVEWGEMPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7M5</accession>
    <name>A0A218P7M5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Arsenate reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_05245</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854014.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7M5"/>
    <dbReference type="GeneID" id="33315653"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05245"/>
    <dbReference type="OrthoDB" id="295776at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046685">
        <property type="term" value="P:response to arsenic-containing substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16345">
        <property type="entry name" value="LMWP_ArsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023485">
        <property type="entry name" value="Ptyr_pPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036196">
        <property type="entry name" value="Ptyr_pPase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43428">
        <property type="entry name" value="ARSENATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43428:SF1">
        <property type="entry name" value="ARSENATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01451">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00226">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52788">
        <property type="entry name" value="Phosphotyrosine protein phosphatases I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0059">Arsenical resistance</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphotyrosine protein phosphatase I" evidence="2">
        <location>
            <begin position="4"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="150" mass="17324" checksum="2B1FF41EB97B6112" modified="2017-09-27" version="1">MEKKLILFVCVKNSARSQMAEAFFNYFNDDPRFRAMSAGTEPAEDIDPLARKVMEEIGISLEGQYPKLYTEEMADKAYLVITMGCLDKCPYAPPEKTWDWGLEDPYGKPIEKYREVRDEIKRRVLKLIEDLKAGKSREEIIGKKSLFSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7S1</accession>
    <name>A0A218P7S1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CARDB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_05545</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854070.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7S1"/>
    <dbReference type="GeneID" id="33315713"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05545"/>
    <dbReference type="OrthoDB" id="86225at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="383"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="392"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="519" mass="56870" checksum="A69C400F7AFDBAA8" modified="2017-09-27" version="1">MRPRSNLLLLVALVLLFLPAVKASDSALFSIAPLNNNFTALPGDTVVVPFRLVNLGSEILENVTVYITGPAGDFLYQNTLISGPIPPGGDYNGTLTIKVLNTALGRYKLFLVARHNSTYSQAPVFVNVKRITDYSLGVDCKERYLIGKPVEVKLRITSKSNGILSGSVGYYIIGPSGTVLNRTTVTFVKPGQSWTQRLLLHDLPLGNYEVHLWANFSGVYKETWGTFQVYRRHLEYKVGFRDGEISVFVYNKTGSGVSDIPVTIGNTTLTTGDDGRLSVPVSEPGTYIVTLDLDGKIVRVPVEVKGLRISYEQNKTRLIVEVRDSNGFPVPNVTVKAVGALDRDYSVTNSSGRAVIDLRKTGYGIIILSAENSNYLGASATAKTFQPPTPTPTETNTTPSNSSPTTMPPITTTMVLTKPPKNYGPLPLILILSGILLAGTSYVAFFRPIVQEETIDRYYFVKVRAPKLKGLDSFSFEKRTNALEVRATKGKAKIEDGRVLWEIEHLEPGEEAYLQVLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7V2</accession>
    <name>A0A218P7V2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ribosomal RNA small subunit methyltransferase Nep1</fullName>
            <ecNumber evidence="10">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">16S rRNA (pseudouridine-N1-)-methyltransferase Nep1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">nep1</name>
        <name evidence="11" type="ORF">A3L08_05830</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Methyltransferase involved in ribosomal biogenesis. Specifically catalyzes the N1-methylation of the pseudouridine corresponding to position 914 in M.jannaschii 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a pseudouridine in rRNA + S-adenosyl-L-methionine = an N(1)-methylpseudouridine in rRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46696"/>
            <dbReference type="Rhea" id="RHEA-COMP:11634"/>
            <dbReference type="Rhea" id="RHEA-COMP:13933"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2 10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase NEP1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="10"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854125.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7V2"/>
    <dbReference type="GeneID" id="33315770"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05830"/>
    <dbReference type="OrthoDB" id="7612at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070037">
        <property type="term" value="F:rRNA (pseudouridine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18088">
        <property type="entry name" value="Nep1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.10:FF:000042">
        <property type="entry name" value="Ribosomal RNA small subunit methyltransferase Nep1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00554">
        <property type="entry name" value="NEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005304">
        <property type="entry name" value="Rbsml_bgen_MeTrfase_EMG1/NEP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023503">
        <property type="entry name" value="Ribosome_NEP1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003205">
        <property type="entry name" value="PRK04171.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003207">
        <property type="entry name" value="PRK04171.2-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636">
        <property type="entry name" value="NEP1/MRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636:SF5">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE NEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03587">
        <property type="entry name" value="EMG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 10" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8 10" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="7 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 10 11" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="196"/>
            <end position="201"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="10">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <feature type="site" description="Stabilizes Arg-xx" evidence="10">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="10">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="10">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="10">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="219" mass="24992" checksum="CAB99845E8B5CF5F" modified="2017-09-27" version="1">MLHLVIAEAEIELVPEPIRDHPAVVNYARKRGKRPDEVLLDSTYHHAALKKLTDGERRGRPDIVHICLLNALESIANKEGKLRVYVHTRNDEVIYIKPETRIPRNYNRFVGLMESLFKNRVVPKDLGLLRMEEKSLEALIKEINPDGVFVMHEKGTFTKPVDFGKRLASLENPAVIVGGFPHGEFKGKILGEKISIYKASLMAWTVVNETIINFEHWVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7V7</accession>
    <name>A0A218P7V7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Crossover junction endodeoxyribonuclease Hjc</fullName>
            <shortName evidence="11">Hjc</shortName>
            <ecNumber evidence="11">3.1.21.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Holliday junction resolvase Hjc</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">hjc</name>
        <name evidence="12" type="ORF">A3L08_05550</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are subsequently ligated to produce recombinant products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).</text>
            <dbReference type="EC" id="3.1.21.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the Holliday junction resolvase Hjc family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.10" evidence="11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854071.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7V7"/>
    <dbReference type="GeneID" id="33315714"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05550"/>
    <dbReference type="OrthoDB" id="34330at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00523">
        <property type="entry name" value="Holliday_junction_resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01490">
        <property type="entry name" value="HJ_Resolv_Hjc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002732">
        <property type="entry name" value="Hjc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014428">
        <property type="entry name" value="Hjc_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040854">
        <property type="entry name" value="Hol_resolv_Hjc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39651">
        <property type="entry name" value="HOLLIDAY JUNCTION RESOLVASE HJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39651:SF1">
        <property type="entry name" value="HOLLIDAY JUNCTION RESOLVASE HJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01870">
        <property type="entry name" value="Hjc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004985">
        <property type="entry name" value="Hlld_jn_rslvs_ar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 11" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 11" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="11">
        <location>
            <position position="30"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="11">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="133" mass="15244" checksum="9C949191FD6C9572" modified="2017-09-27" version="1">MRYRTGASAERELIKMLEKAGFAVVRSAGSKKVDIVAGNGRLYLCIEVKSTHDDTLYFREEDYEKLTSFAERFGGKAVIAVKFINRGWRFFYPENLRKGGKNYKLDVQTKEYLTFDEITGRQASLVGVINRET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P801</accession>
    <name>A0A218P801_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lipid A biosynthesis N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854176.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P801"/>
    <dbReference type="GeneID" id="33315826"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06115"/>
    <dbReference type="OrthoDB" id="101010at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="91" mass="10170" checksum="F8A188E897292210" modified="2017-09-27" version="1">MNGGEIIGLIGMLLLVGSWVPQTLETVRKRHCPLNLEFIVIYVTAASMLTVYSYIIGDWIFFTLNFLSALQSAINLVVKLMELKGKIKSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P832</accession>
    <name>A0A218P832_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uracil permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_06155</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854183.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P832"/>
    <dbReference type="GeneID" id="33315834"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06155"/>
    <dbReference type="OrthoDB" id="76842at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042907">
        <property type="term" value="F:xanthine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006042">
        <property type="entry name" value="Xan_ur_permease"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00801">
        <property type="entry name" value="ncs2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037981">
        <property type="entry name" value="NCS2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42810">
        <property type="entry name" value="PURINE PERMEASE C1399.01C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42810:SF2">
        <property type="entry name" value="PURINE PERMEASE C1399.01C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01116">
        <property type="entry name" value="XANTH_URACIL_PERMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="324"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="352"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="384"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="410"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="433" mass="44794" checksum="46830CA00D7337FE" modified="2017-09-27" version="1">MENVEVMEKPVLKVGIEEKVEPAKALVFGLQHVLAMFGATVTVPLVVGGAIGLSGDQIALMIQAVLLAMGIATLLQTTIGSRYPIVQGSSFAFIPGLISIGSSLGMAAVQGALLIGGLIEAMIGWLGIIGKVRKLFTPLVTGVTITLIGFSLADVAIKNFFNFYADPAGETLVKSSAVALVTFLTTVFVALRAKGSLKAMPVVVGALVGYLISVPFGLTDFDLVKSLPVLGVPSIFPWGAPVFDASAIALLLFAFMVSIIESVGDYHAIATVTGSEITEEHIARGIGSEGLACSIAGLLGACGTTSYSENIGVVALTKIGSRHVVQVGAVMLIFLSLLPKFAGILASMPAPVLGGLTLALYGMISVTGLRLIKEKVEFTDRNTLILAAALIAGLGAPQLPPEFLEHFPKLIASILESGMAVGALTAIILDRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P837</accession>
    <name>A0A218P837_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF354 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06270</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854204.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P837"/>
    <dbReference type="GeneID" id="33315857"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06270"/>
    <dbReference type="OrthoDB" id="185087at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007152">
        <property type="entry name" value="DUF354"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39662:SF1">
        <property type="entry name" value="DUF354 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39662">
        <property type="entry name" value="DUF354 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04007">
        <property type="entry name" value="DUF354"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005357">
        <property type="entry name" value="UCP005357"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="376" mass="42008" checksum="23FF48DDAD4679C2" modified="2017-09-27" version="1">MKVWVDITNAPHVHFFKGVIRELEKAGHEVLVTTREFDGLTGILDMYGFDYYVVGRHGGATLEGKLIAGTERMYRLSKLIVEEKPDLAIYKHSAEAPRVAFGLQIPSIGFVDNETAIAQNKLILPYTKLLLFPKAIDAYDLLKCGADPNGMRPVNGFSELAHLYGFRPDRRVLTELGVKRHGYIVMRTEPVKANYFNGPEKSVLEDVIPLLPDVPIVLFPRTEEQRARFERFDNVIMPERPVDSLSLLHYAKLMIGAGGTMNREAIALGTPTISTYPGRLLAVTRWLVEKGVKFHSTDPVKVAAMAERMMELNGSYRSYIRSVVSGFENPMNVILEEIETYEESGMFMAGLEGQSEAGNLGGYVGLDQGGNEEKRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P858</accession>
    <name>A0A218P858_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Dihydrodipicolinate synthase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_06290</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854208.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P858"/>
    <dbReference type="GeneID" id="33315861"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06290"/>
    <dbReference type="OrthoDB" id="33636at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008675">
        <property type="term" value="F:2-dehydro-3-deoxy-phosphogluconate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008840">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00408">
        <property type="entry name" value="DHDPS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020625">
        <property type="entry name" value="Schiff_base-form_aldolases_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128:SF66">
        <property type="entry name" value="4-HYDROXY-2-OXOGLUTARATE ALDOLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="DIHYDRODIPICOLINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00666">
        <property type="entry name" value="DHDPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="5">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001365-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001365-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="293" mass="32396" checksum="9B1559DAE0AF7881" modified="2017-09-27" version="1">MRGVIVPLVTPFNEDYSIDVPALEEHVEYLQKAGVHGIFINATTGEFTSLSTEEKKFLAEKGRELVTSAFYLVGTASSNTFEVIELTRHAQDIGADYVVIAPPYYCPLGEEALFKHYSMVAEKTDLPIILYNIPSCANSLSVPLIKRLALEYSNIAGVKETIDSVNHIRDVILEVKGERKDFKVFTGLDQHFLNTLILGGDGGIMACANFAPEIHLAVWKAFQEKRFEEAFEGARKLAKLSKVYDLASSFGSAIKLAMSVRGFSIKPVLRPPYVMDGEETKEEIGKLLAEILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P878</accession>
    <name>A0A218P878_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein uS15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rps15</name>
        <name evidence="9" type="ORF">A3L08_06380</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2 5">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854223.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P878"/>
    <dbReference type="GeneID" id="33315879"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06380"/>
    <dbReference type="OrthoDB" id="6533at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.10:FF:000003">
        <property type="entry name" value="40S ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.130:FF:000001">
        <property type="entry name" value="40S ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_A">
        <property type="entry name" value="Ribosomal_uS15_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_uS15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023029">
        <property type="entry name" value="Ribosomal_uS15_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012606">
        <property type="entry name" value="Ribosomal_uS15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006331">
        <property type="entry name" value="PRK08561.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885">
        <property type="entry name" value="RIBOSOMAL PROTEIN S15P/S13E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885:SF6">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08069">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01386">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00362">
        <property type="entry name" value="RIBOSOMAL_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS15 N-terminal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="151" mass="17361" checksum="CEF24219F59DC616" modified="2017-09-27" version="1">MARIHARKRGKSGSKKPPRTAPPTWVEYTAEEVEGLVIKLRKEGYSAAMIGTILRDQYGIPSVKLITGKKITKILEENGLAPDIPEDLMALIRKAVNLRKHLEQHPKDKHSMRGLQLTESKIRRLVKYYRRTGKLPAKWRYDPEQAKLLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8C3</accession>
    <name>A0A218P8C3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Vitamin B12 transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_06615</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854267.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8C3"/>
    <dbReference type="GeneID" id="33315926"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06615"/>
    <dbReference type="OrthoDB" id="24039at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01143">
        <property type="entry name" value="YvrC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050902">
        <property type="entry name" value="ABC_Transporter_SBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054828">
        <property type="entry name" value="Vit_B12_bind_prot"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038402">
        <property type="entry name" value="TroA_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30535:SF34">
        <property type="entry name" value="MOLYBDATE-BINDING PROTEIN MOLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30535">
        <property type="entry name" value="VITAMIN B12-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53807">
        <property type="entry name" value="Helical backbone' metal receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="3">
        <location>
            <begin position="85"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="3">
        <location>
            <begin position="356"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="24"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="25"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="622" mass="67510" checksum="048A86881403CA74" modified="2017-09-27" version="1">MKRTALLLIVLFLGAVVASGCISGETTTKTPSPTTSVPETTSSFTPTETTTTSSPTATETEKPYYPLTITDFSNRTVTIEKEPRRVVTLAPSITEDLYYLGLFDRVVGVTQFDDFPPEVSNVTRVGGYGKYANLEIIASLNPDIILVDSYSMAILDELEKIAPVVVVDPHSMDDIPLALELLGKVFNAENNARKVISEFKSNIEAINSTVKGYPKLSVFYVVWNNPLMTAGGGTFISDVIELAGGRNVFNDTTGWPTVSQEQVIERNPEVIILTPHCGMTVQDVYNGPLANTKAARDGKVYVIENENDLIHPSPRVLEGLKTIAGLLHPEAFKVSYPLTVTDFEGRTVTIDKEPQRIVSLAPSITETLFYIGAGGKVVGVTDYDDFPPAVKNITRVGGYGKYANLEIIASLNPDLIVADDFSMGILDSLEKIAPVIILSPKSIDDVYRKVELLGKVTNREEGARAVVADMKARVSYVTSMVAGKQKVRTFFLLSYYNGYWTAGNGTFINDLISLAGGENIFADISGWGAANAEQIIKRNPEVIIISPNAGIKPEDLCSGPLSNVDAVKNGRVYIISDENLVVRPGPRIVHGLEEIAEYLLHPEAFNYQPQPLVCNATSSASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8G4</accession>
    <name>A0A218P8G4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_06995</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854336.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8G4"/>
    <dbReference type="GeneID" id="33316002"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06995"/>
    <dbReference type="OrthoDB" id="8638at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08023">
        <property type="entry name" value="GH16_laminarinase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF55">
        <property type="entry name" value="GLYCOSIDE HYDROLASE FAMILY 16 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH16" evidence="3">
        <location>
            <begin position="62"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="28"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="28"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="340" mass="38111" checksum="7AE9F771B9560A8A" modified="2017-09-27" version="1">MDKKLLGLLILVVMLGSFISGCIGGETQTTPSSSWTSTTQTTSMAPLDSSTTRTTTTTATTTTTSKTTTPTTTTSTAQVPAEIQENGRRWKLIWHDEFEGEKVNENYWTFEIGNGGAYGIPGWGNNELEYYSPNNTRIENGMLVIEARKEWATDEYGTYMYTSSRLKTEGKAEFAPPVRVEARMKLPKGKGLWPAFWMLGSNIREVGWPQCGEIDIMEFLGHEPRTVHGTVHGPGYSGGRAITKSYTLPEGVPDFTEDFHVFAIDWFPDRIEWYVDGTLYHVVTKEQVEAMGKEWVFDKPFYIILNLAVGGNWPGPPGATTKFPARMYVDYVRVYKLAED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8I1</accession>
    <name>A0A218P8I1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Peptide ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_06955</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854329.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8I1"/>
    <dbReference type="GeneID" id="33315994"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06955"/>
    <dbReference type="OrthoDB" id="44105at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43376">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43376:SF1">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="200"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="302"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="107"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="335" mass="37620" checksum="46933F18BE7C1FA6" modified="2017-09-27" version="1">MRPIYKYLLIKLSFLLATYFVAVTIAFVLPRIAPGNPVQQIVAGLSQGSLSPELLDQYQKRLVEEFGLNKPLYVQYLEFLKNAFSGDLGTSISSYRQPVTQLIAWHLPWTLLLLVPATLVAWTIGNYLGARAAYRRGTLFEKTVVSTGIILSQIPYYWMAMVLIYVFAIKLGWLPSGSAYDPTLAPSLNWEFIKSYLRHYILPFLSIALVSTGGWIIGMRVLAAMELGSTYVKFSEMLNVDGEIIFRYVLRNSLLPQVTGIAIQLGTVMAGQIITEQLFNYQGMGILLARALGSRDYPMIQGIFLILIGTLLLANFIVEFIYVLIDPRIRLGARE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8I3</accession>
    <name>A0A218P8I3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Sodium:phosphate symporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_07035</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Potential transporter for phosphate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_198362102.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8I3"/>
    <dbReference type="GeneID" id="33316010"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07035"/>
    <dbReference type="OrthoDB" id="101311at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101:SF80">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="237"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="283"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ07093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="305" mass="30854" checksum="E4E76589B85EFB63" modified="2017-09-27" version="1">MVLLASALFMAWAIGANDSAKAVGTAVGSGVIGFKRAVLLIGVFVTLGVFLGGAGVSGTISGMAEGMNPTHVGLVLFSAATAVTLASLWGRPISTTQSIIGGLVGASLALGLPVNWWTVGKIVSAWVLSPLIAALFAVAVYRIYKPAMKGIKGLRNLELAQKWLVFTAAAYSSFNLGANELSNVAGLLQSLGVDGPFKLVLALTLALGALTFSYNVMMTVGRELAPLGPTSAFSSQFGASLAVSAANLFGLPVSSGQAIVGAISGLGLYKGENVNLGLLVGIARGWVVAPIAAGALSYVMITILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8J2</accession>
    <name>A0A218P8J2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_07095</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854353.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8J2"/>
    <dbReference type="GeneID" id="33316022"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07095"/>
    <dbReference type="OrthoDB" id="85959at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_transp_PIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36838">
        <property type="entry name" value="AUXIN EFFLUX CARRIER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36838:SF3">
        <property type="entry name" value="TRANSPORTER AUXIN EFFLUX CARRIER EC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="270"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="292" mass="32370" checksum="D3C529E151DD441B" modified="2017-09-27" version="1">MNIVEMLALIALGYILKLALKDERPFDWLRRITNDVLLAFFVFGNVASKDLAYLLSIKTVFLYVFIIIAISLGFSYLYGRFVLKNDPWAGALMVLSVYPNTAALGFPIASLFLSDITPAILYSTTNSMIVIPIVTFIAAHYSSGGASVKESFLKALKFPPTLGNLLALGLVIAGIKLPGWFIEPIKTIGWWSIPLLLIYFGSRITLRAFELRKLLEVGTFRIAIPFVFVFLTLRWASRDVFYSVLVEASMPPAIAANAILAQYRLKAEEAISVTFVLTLLVIGLFMALKALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8J7</accession>
    <name>A0A218P8J7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transglutaminase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_07060</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0252 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854346.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8J7"/>
    <dbReference type="GeneID" id="33316015"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07060"/>
    <dbReference type="OrthoDB" id="86147at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.620.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007562">
        <property type="entry name" value="Transglutaminase-like_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04473">
        <property type="entry name" value="DUF553"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="208"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Transglutaminase-like" evidence="3">
        <location>
            <begin position="296"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="577" mass="66753" checksum="B1ED52474EE8E66A" modified="2017-09-27" version="1">MSEKDLHYLITRIINPHLNWIIASLRSNNRVLHGRDGYTSIHWVDHTLSDTFASELEGWRDNLWYKYKEDFLERGAEILDSLLEIMIRIEAKLPDNDSKSRIMSDLWRMAGEIEGYLELNGYLESRKEYFTNKALEIRKRDEELRRKLEEQKKRADIGSRASKRLAGKSIFESERKIVHPHNKRTVSPNTFQENPNKKRKWKFPWKKFFGIILVFLVFFALYDLSHTNGRLVRQVIPDEIEAKISEFWGTAKNFTGETASVKSLTSTAVACSRADYSLEAALECYLNDPDEFQVLGPLASRLKGSTLQESSWNILEWEDQNIQYDWAKYNGLGSTKIQKPSETIQSGSGVCVDYAVLTAGLLLAMNYSPVYVLEIDFSNDQMGHAAAAIKINGQFFVIDQHPPIMDLGTYWKYWAYWRSEYSGGTLKNLIISSAKVYEARLESEKVVVDYVGTLTGEEFKKHDYTFSETDLTRLISDLRARFLREFPNLQLDSKISNLDTTAYLPYGYSAGVTWKTTFPDFTEHYNPIFHDEFVDYMYSRITGSRRVVSDLGTYNRFWVKGQIEGDNVKVVLCLAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8N2</accession>
    <name>A0A218P8N2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Restriction endonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854403.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8N2"/>
    <dbReference type="REBASE" id="210321">
        <property type="entry name" value="TpaP4McrBC2P"/>
    </dbReference>
    <dbReference type="GeneID" id="33316074"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07355"/>
    <dbReference type="OrthoDB" id="9837at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052934">
        <property type="entry name" value="Methyl-DNA_Rec/Restrict_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37291">
        <property type="entry name" value="5-METHYLCYTOSINE-SPECIFIC RESTRICTION ENZYME B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37291:SF1">
        <property type="entry name" value="TYPE IV METHYL-DIRECTED RESTRICTION ENZYME ECOKMCRB SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="1">
        <location>
            <begin position="407"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="716" mass="80910" checksum="5C302B78DACB7379" modified="2017-09-27" version="1">MIRPVPVKTSMYLINRFLDAEYGDFGFVIEDMKLFPNGKYIAVLVRLDDDSRFIHVFGTGGQPVGGWEKGLPVPCADEAIMLPGEEKLAVFTKCGTGEVRIYPVQSADPESEVLPVNFTRLPFYVSFTPRERLVVFCDYKVEYFAPGEVEVIKIPTPQDGFAYIHSVKDTDDSLYLLYTFRKNQRQRLELAKIPLPMFPYYSSLEFWDGIEVISKSSIVPRGGRQIGDIWLKDGSVMATLGTKGGNEVHIVGSAGAKTVLLPGELVFLSFTSRGLFVITGVYGGNLRAGFISFDVLNTSKKIGAEDFQGEVVLGRYVPGVVDPKFAGISRDGETLYFGRSGGKSSGGTFYYFLRREVDYLYLLEAPKVQSGDLEPEGTASEAHGGGHQKEGLDENLGETVLISLLRRFKQVIIYGPPGTGKTHLALKTAKNAEFVTFHQSYSYEDFVEGFRPIEKEEKVVYTVSDGVFKRLAVRAIYDSLPDGFRRRGENMEYEDMKMAVQEFLKKRRAGDKTPIVPRREFYIVIDEINRGNISRILGELITLLDPDKRLGAPTEVIVTLPYSRELFALPPNLYIIATMNSADRSIALLDIALRRRFAFVELSPDPEKLRDVNVDGINLEHLLTRINSIIEQEKGKDYTLGHGYFLDIPSADDPRQALYMVFYHKVLPLFQEYFYGNWEHLRSLYPGFEFIDERGRIIWMDLDEFIDALRRLVGSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8N9</accession>
    <name>A0A218P8N9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Translation initiation factor IF-2B subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_07130</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854360.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8N9"/>
    <dbReference type="GeneID" id="33316029"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07130"/>
    <dbReference type="OrthoDB" id="45195at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.420">
        <property type="entry name" value="translation initiation factor eif-2b, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051501">
        <property type="entry name" value="eIF2B_alpha/beta/delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027363">
        <property type="entry name" value="M1Pi_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006210">
        <property type="entry name" value="PRK08335.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45860">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45860:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="275" mass="30622" checksum="CE74C7EE9931E7E1" modified="2017-09-27" version="1">MLPQEIRIILEEMRSERIRGASWLAMKGAEAYIALADLLEGEELENALREMKTEIPAVNRTMASLYNLSRFIPVTGNPEVVRAKAEEFIRLAEEARREIGNIGGELIDENEVVITHSFSSAVLEIFKAAGRKGKHFKVVLTESAPDYEGLALARELEKLGIPFEIITDAQLGLFAEKATLALVGADNVTRDGAVVNKAGTYLLALACHDNGVPFYAAAESFKLHPVMTSRDVELVERPYARQGHRIRNYLFDVTPWRYVRGVITEFGVLVPPKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8P5</accession>
    <name>A0A218P8P5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Glutamine--fructose-6-phosphate aminotransferase [isomerizing]</fullName>
            <ecNumber evidence="3 11">2.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">GFAT</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Glucosamine-6-phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Hexosephosphate aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">L-glutamine--D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">glmS</name>
        <name evidence="14" type="ORF">A3L08_07285</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="3 11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854389.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8P5"/>
    <dbReference type="GeneID" id="33316060"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07285"/>
    <dbReference type="OrthoDB" id="372195at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046349">
        <property type="term" value="P:amino sugar biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006047">
        <property type="term" value="P:UDP-N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00714">
        <property type="entry name" value="GFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000001">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000002">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000006">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00164">
        <property type="entry name" value="GlmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005855">
        <property type="entry name" value="GFAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047084">
        <property type="entry name" value="GFAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01135">
        <property type="entry name" value="glmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001484">
        <property type="entry name" value="PRK00331.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937">
        <property type="entry name" value="GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE, ISOMERIZING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="GLUTAMINE--FRUCTOSE-6-PHOSPHATE TRANSAMINASE (ISOMERIZING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 14" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 11 14" id="KW-0808">Transferase</keyword>
    <feature type="initiator methionine" description="Removed" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="12">
        <location>
            <begin position="2"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="13">
        <location>
            <begin position="280"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="13">
        <location>
            <begin position="453"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for GATase activity" evidence="11">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="active site" description="For Fru-6P isomerization activity" evidence="11">
        <location>
            <position position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ07138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="602" mass="66140" checksum="A5E1148013073DD4" modified="2017-09-27" version="1">MCGIIGYIGDRPACQVIVKGLKRLEYRGYDSAGIVTEDGRRLFIKKGAGRIDELIERLGLLEMPGKRGIGHTRWATHGVPNDVNAHPQTDCTGRIALVHNGIIENFAEIKDELVAKGHRFESDTDTEVIAHLIEEELRGESDFEQAMRKALLKLRGSFALGIIYTGEPDRLYFVRNESPLVLGIGDGENFAASDVPAFLEYTNRVVFLDDREYAVITKDSWVVKNLDTGEVVEKPVHEIGWTLEMAEKAGFPHFMLKEIYEQPRAIKDAIHGNADVINAVAREIAKYDKIFIVAMGTSYHAGMVGRYLFQRLAKKVPIVEDASEFRYEFEDIIDEDTLVIAITQSGETADTLAAMKLAKKKGAKVLAIVNVVGSMATRIADLTLYTHAGPEIGVAATKTYTTQLTVLTMLAIELARILGTADEDYLDELEAGLKAVPNLVEEVLKHDGGIKELAESLVDKRDFFYIGRGVSVPTALEGALKLKEISYIHAEGLSAGELKHGPLALLEDGVPVVAVAPSGKTFDKIVGNIEESRARGAFIISLGDSEELGRVSNAFIRMPDMDELLSPIVYVVPLQLIAYHMAVLRGNDPDKPRNLAKSVTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8S7</accession>
    <name>A0A218P8S7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854402.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8S7"/>
    <dbReference type="GeneID" id="33316073"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07350"/>
    <dbReference type="OrthoDB" id="85471at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006120">
        <property type="entry name" value="Resolvase_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02796">
        <property type="entry name" value="HTH_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase HTH" evidence="1">
        <location>
            <begin position="85"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="133" mass="15471" checksum="82EBAE49771B93C5" modified="2017-09-27" version="1">MRAEIKARNNEELIRKLRESLSDEVTEVYINLRPTKEILVRILERAPNVRKISCPPSLYPKVSKKVIAALAQMGIELVPEGYPRGRPRKYDERTVRQVRELLRKGVPAKEISSRMGIPLRTVYYMIEQVEKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8W3</accession>
    <name>A0A218P8W3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Flavin prenyltransferase UbiX</fullName>
            <ecNumber evidence="6">2.5.1.129</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ubiX</name>
        <name evidence="8" type="ORF">A3L08_07795</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Flavin prenyltransferase that catalyzes the synthesis of the prenylated FMN cofactor (prenyl-FMN) for 4-hydroxy-3-polyprenylbenzoic acid decarboxylase UbiD. The prenyltransferase is metal-independent and links a dimethylallyl moiety from dimethylallyl monophosphate (DMAP) to the flavin N5 and C6 atoms of FMN.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>dimethylallyl phosphate + FMNH2 = prenylated FMNH2 + phosphate</text>
            <dbReference type="Rhea" id="RHEA:37743"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:87467"/>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
            <dbReference type="EC" id="2.5.1.129"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the UbiX/PAD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.129" evidence="6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854475.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8W3"/>
    <dbReference type="GeneID" id="33316161"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07795"/>
    <dbReference type="OrthoDB" id="9540at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106141">
        <property type="term" value="F:flavin prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1950:FF:000001">
        <property type="entry name" value="Flavin prenyltransferase UbiX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01984">
        <property type="entry name" value="ubiX_pad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004507">
        <property type="entry name" value="UbiX-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004685">
        <property type="entry name" value="PRK06029.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00421">
        <property type="entry name" value="ubiX_pad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43374">
        <property type="entry name" value="FLAVIN PRENYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43374:SF1">
        <property type="entry name" value="FLAVIN PRENYLTRANSFERASE PAD1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 6" id="KW-0288">FMN</keyword>
    <keyword evidence="1 6" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Flavoprotein" evidence="7">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="9"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="79"/>
            <end position="82"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>dimethylallyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>dimethylallyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="177" mass="19224" checksum="7DE8F90F9C8A0469" modified="2017-09-27" version="1">MKVIVAITGASGAVYGLRLIEVLRELGHEVVTVASKTGLAVVRHETGVDFKPDYTEENLFAPIASGSNPFDAMVIAPCSMKTLSAIASGYADNLIARAADVALKERRKLILLVRETPLNLIHLQNMMAVTQAGGIIMPASPGFYTKPKTLDDMINFIIGKILDLLGIENEVYPRWRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8X4</accession>
    <name>A0A218P8X4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">TldD-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_07865</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U62 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854489.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8X4"/>
    <dbReference type="GeneID" id="33316178"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07865"/>
    <dbReference type="OrthoDB" id="98233at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2290.10:FF:000003">
        <property type="entry name" value="Zinc-dependent protease, TldD/PmbA family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="entry name" value="PmbA/TldD superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051463">
        <property type="entry name" value="Peptidase_U62_metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025502">
        <property type="entry name" value="TldD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30624">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN TLDD AND PMBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30624:SF11">
        <property type="entry name" value="ZINC-DEPENDENT PROTEASE, TLDD_PMBA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004919">
        <property type="entry name" value="TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="Putative modulator of DNA gyrase, PmbA/TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloprotease TldD/E N-terminal" evidence="5">
        <location>
            <begin position="20"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloprotease TldD/E central" evidence="7">
        <location>
            <begin position="114"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloprotease TldD/E C-terminal" evidence="6">
        <location>
            <begin position="232"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="473" mass="53348" checksum="5AA29AFB8B4C84D7" modified="2017-09-27" version="1">MHELVEFAVEKALELGASYAEARFEEKNGTSLTMKNGSPEGLEVLAEKGMGIRVLVDGGMGFASTNVLTKESVAEAVKRAVKLAKAASRVRNEPIRFSEENFHRISYKVKMKKDFRDVSPEEKLELLRKIEEEVKATGVNVPMRFIRYYDQVWHKIIANSDGAFIESVIPRISTTYNLVVFENGQMEQAPFVQRAFSGGLELIEKDEPWKWAVKDVQALKKLIYEGKKPPEGKVDLVISPEVAGIAVHESVGHPYEADRILGREAAQAGESFVKPEMLGERIGSEVVTVIDDPTIPNSWGFYLYDDEGVKARPRYLIRNGIITEFLTNREYAAKLGQRSNAAARAINYNREPIVRMANTYLAPGDHSFEELIEDIKLGVYMVSFNEWNIDDRRYQQRYIGREAYLIENGEIKHPVRRPILEITTRALWSSVDAVGKDVEFYPGTCGKGEPGQGVPVWMGGAHARLRDIPLRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Y0</accession>
    <name>A0A218P8Y0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0166 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854482.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Y0"/>
    <dbReference type="GeneID" id="33316170"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07830"/>
    <dbReference type="OrthoDB" id="8505at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003793">
        <property type="entry name" value="UPF0166"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35983">
        <property type="entry name" value="UPF0166 PROTEIN TM_0021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35983:SF1">
        <property type="entry name" value="UPF0166 PROTEIN TM_0021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02641">
        <property type="entry name" value="DUF190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="GlnB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="126" mass="14480" checksum="0BBD032776998259" modified="2017-09-27" version="1">MVEVEHWNTLRLKIYIGENDRWEGKPLYKAIVEKLREMGIAGATVYRGIYGFGKKSRVHSADVMRLSTDLPIVIEVVDRGYKIEKAICEIKPMIKDGMITVEPIIVVWVGTSEEVKKFEEDAIREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P908</accession>
    <name>A0A218P908_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854522.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P908"/>
    <dbReference type="GeneID" id="33316215"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08050"/>
    <dbReference type="OrthoDB" id="9837at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052934">
        <property type="entry name" value="Methyl-DNA_Rec/Restrict_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37291">
        <property type="entry name" value="5-METHYLCYTOSINE-SPECIFIC RESTRICTION ENZYME B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37291:SF1">
        <property type="entry name" value="TYPE IV METHYL-DIRECTED RESTRICTION ENZYME ECOKMCRB SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="1">
        <location>
            <begin position="128"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="377" mass="44330" checksum="DEAFB8A28B08DD1F" modified="2017-09-27" version="1">MKEDGISGLRDIPRTEKGALSLAQTTDDPRVLESLYNDHKENPRVVKAIVNNPYCPSNIKRDYLDYVLNDYLRAYRDRLRTLITDWDDLLDAMNICLEVWNPPREIAPIPEINLEDPRMREVFEAIENYKQVILYGPPGTSKTYYAKGLALAITNDPNNVELVQFHPSYSYEDFVESIFPEVDEETKIIKFNIKDKVFKKLCKKADANPDKKYVLIIDEINRADLSKVFGEIFSALEYRKEAIRLLYSGEKLTIPSNLYIIGTMNTIDKSTVDLDFALMRRFKFFEVNPDPNVLRKMLVKNEVEEELIEKIINVFKQIQEYYPLGHAYFKDVKSKQDLKLLWNHQLSFLLKEYFTDLQKETYETIKRIYWEGLELED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P911</accession>
    <name>A0A218P911_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Iron ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_07980</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854511.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P911"/>
    <dbReference type="GeneID" id="33316201"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07980"/>
    <dbReference type="OrthoDB" id="24644at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03214">
        <property type="entry name" value="ABC_Iron-Siderophores_B12_Hemin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000134">
        <property type="entry name" value="Iron-enterobactin ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42794">
        <property type="entry name" value="HEMIN IMPORT ATP-BINDING PROTEIN HMUV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42794:SF1">
        <property type="entry name" value="HEMIN IMPORT ATP-BINDING PROTEIN HMUV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="4"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="248" mass="27264" checksum="25ECA5112A91F5FC" modified="2017-09-27" version="1">MKAIKARNLSFSYNGADVLKGINLEVEEGEFLAILGPNGAGKSTLLKCISGILNCRGVEVFGKPIGEYSRNELARIIAYVPQRTEPGFMTVFDTVLLGRRPYMGLRPSKKDLEAVKRALERMGIAGFALKITNKLSGGELQKVGIARALAQEPKILMMDEPTNNLDIRSQLEVMRLAREFSAEGGTVIMVMHDVNLALRFAGRFVFMKNGKIVADGGREILKPSIFREVYGVDVIIEEVRGIPLVVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P972</accession>
    <name>A0A218P972_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_08310</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854570.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P972"/>
    <dbReference type="GeneID" id="33316268"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08310"/>
    <dbReference type="OrthoDB" id="46222at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06423">
        <property type="entry name" value="CESA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43630">
        <property type="entry name" value="POLY-BETA-1,6-N-ACETYL-D-GLUCOSAMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43630:SF1">
        <property type="entry name" value="POLY-BETA-1,6-N-ACETYL-D-GLUCOSAMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="5"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="53"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="343"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="385"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="4">
        <location>
            <begin position="93"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="452" mass="52452" checksum="C72797E3AFF7EF68" modified="2017-09-27" version="1">MRRKVLSYLSAFIISAYVVYLDYSFLQWSSWRAVLFIFGRAVDRYSVYSFVRYLFYLTGAFIIFLYTAYFIFYLYYRSNEGLIKPPRFYPRVSIVVPAHNEALTIERLIESIEYQDYPYNLVEVIIVDDGSEDGTGEIASRYGVKVIRHEKNLGKAQSLEDGIKSAKGDVIITMDADSYFGNGYSLRRIVESLYERSLVGISTGVIRIERRFGNWIEKFQEIEFLHSFELGRRIQGYLGWLLVIPGAFSAFKGYFIKSLPAIPKDTLAEDFDLTVIAFRAGLDSNFEPGAVVYTEPEQSWRRLYRQRIRWYYGGLQVLRKHDDLILNPKYGEKGIFMFLHSIVLEYVLAVIQITGIILLPVIVLFRHYLGISITDINLPPDIMVFLFLFVFFIQYIPGVFMSGTLMVIERGSKEALRNIPTIFLYYLFYNPLLSLAKLDATLRFLRGVAQGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9A5</accession>
    <name>A0A218P9A5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_08290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854566.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9A5"/>
    <dbReference type="GeneID" id="33316264"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08290"/>
    <dbReference type="OrthoDB" id="96447at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="268" mass="29900" checksum="D988471DDF23790F" modified="2017-09-27" version="1">MKRKNTTVEIAAPKEAVEAVLNDAPSFITNWPYVVRVSMKDGLKAEIMLPRFIFKFRDVYRFEYHSDYNSHIYDGTGEKGHISLVVTLKEWRKNTSAVLELSYKGKGEFWLGKTLQDFVEKIGSVLKEMAENRPVQEQVQVPAGTKESIAVNVDFADPMSVASFLSRSRMVQSGLHIIGEKGLFDIISELRATIPDRILYISGITSDGSSSFKVLLDGSRILAIEHRTSEGVEVVKVENDEDARRALEIASGIKGAYMVNVWVPIGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9E3</accession>
    <name>A0A218P9E3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9E3"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08690"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="523" mass="60222" checksum="59E9F3127C64028F" modified="2017-09-27" version="1">MLYTKRKEDIEKALEWLRSSEVCFDDGSFASIRRGPMITTVIYYLQHHLGYKDGVSLEELKAFLYCMLLSEINKWEKTVKAIAWCPIEQTLEEISGHVIKVHNGKFIVDPEKVVGNSGIPPYWRALEPVVLSAKKICNDILNPPNERIEKLLSQPPQSLQKDPEMEEYLSRIRYEINHETPSRLEKNRKDQPSFENKPMPKVWSRQNDLKKFVKPEGITAQEVLREYSDPDILRDIEKAYEIAWQKEKLLYGEGYIKFLVSMAEYYLEEHSMRLRSELARLLDEIFGSNTRKKAAEYYIKAARIATEISLIYFPKLNYPEEAEKYLKLAVDLEEKAIELGFTPEYHSITLNNLGTHYYETNRPREALRVLKKALKYASTPDEKGLILHNLALTYADLGMKTEAVESMVKSICIHYAAQHDFGDVSLYDDGINRIIEMTGDPNTDIQALRIALDFIGGNLNATEAVEYLQRINTSKWPLSKALLRALTDGDVSLPDSVSECKKLLENVTKAREHTARRSMGVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9E8</accession>
    <name>A0A218P9E8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4Fe-4S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854661.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9E8"/>
    <dbReference type="GeneID" id="33316372"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08830"/>
    <dbReference type="OrthoDB" id="5817at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23947">
        <property type="entry name" value="PAPS_reductase-like_YbdN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07953">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050128">
        <property type="entry name" value="Sulfate_adenylyltrnsfr_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010367">
        <property type="entry name" value="PRK13795.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010369">
        <property type="entry name" value="PRK13795.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43196:SF2">
        <property type="entry name" value="PHOSPHOADENOSINE PHOSPHOSULFATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43196">
        <property type="entry name" value="SULFATE ADENYLYLTRANSFERASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="567"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="598"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="631" mass="72357" checksum="B70582931299B025" modified="2017-09-27" version="1">MGRPVFLGKAYINWCEECNVPLIGDSCAVHGKEALFRLDITPPGDLRFAFEKDIEFIRSVFKEHYGVDVGELFDGKIVLLSKTPGEDDVYEIILDGYVFGWIRFDPLELRWKPGLKVEGAIALWKRFGKNMKKWIIVDRGAVEPILSGSNLLPVGITEAEPSIRRGDDVILISEDCEVIATGIAKKDYEGLANKERGTGVKVRRQKSVNYREGKKATMEDVLRANSIELEKKVEEARRFMRKTANRYSDLPVAVAFSGGKDSLAVLGLALEEFSEDGFTVFFNNTGIEFPETLEYVEELKKELEPKGIKFIAADAGDAFWRAIHVFSPPGRDYRWCCKVTKLGPITMAIKENYPQGVLMFVGQRKYESIKRFKQPRVWRNPWVPNETGASPIFHWRALEVWLYIFSRKLKYNPLYEDRLDRIGCFLCPSASLAEIYTLKEEKPELWAKWESELKRWQKRFNMPDEWITYGFWRWKKLSKGEKAIARELGVEVPEERSWEPVKYSMEETEDGTFIVRFNTVVNLNRIREVAPILGEVEEGENYIRAGDVVFREDGAYAEDFNEGVQAYHLVKRAYECVGCGVCVGKCPEGALSIDPRSKKIVVNYDLCTHCRECMDVCPLLKIKNPEEGSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9G1</accession>
    <name>A0A218P9G1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Biotin transporter BioY</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_08920</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the BioY family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854677.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9G1"/>
    <dbReference type="GeneID" id="33316390"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08920"/>
    <dbReference type="OrthoDB" id="50443at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015225">
        <property type="term" value="F:biotin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1760.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003784">
        <property type="entry name" value="BioY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34295">
        <property type="entry name" value="BIOTIN TRANSPORTER BIOY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34295:SF1">
        <property type="entry name" value="BIOTIN TRANSPORTER BIOY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02632">
        <property type="entry name" value="BioY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016661">
        <property type="entry name" value="BioY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="1 2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR016661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="173" mass="17902" checksum="5E68F3D09E3EFC15" modified="2017-09-27" version="1">MNARDVAFAGLFAALTAVGAQIAVPIGPVPFTLQVLLVLLSGLVLGSRLGLVSQLVYLVAGAVGFPVFAEFSGGFAHIYGPTGGYLLAFPIAAFLAGYITEKTGESVWGMTAGSLFGVVVIYALGWLRLCLFMAGDFGKAFKLGVLPFVPFDVLKAGIAVGVAKTVRKMVEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9I8</accession>
    <name>A0A218P9I8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chromosome partition protein Smc</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">smc</name>
        <name evidence="8" type="ORF">A3L08_09055</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for chromosome condensation and partitioning.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">Contains large globular domains required for ATP hydrolysis at each terminus and a third globular domain forming a flexible hinge near the middle of the molecule. These domains are separated by coiled-coil structures.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SMC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854700.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9I8"/>
    <dbReference type="GeneID" id="33316417"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09055"/>
    <dbReference type="OrthoDB" id="9143at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01894">
        <property type="entry name" value="Smc_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011890">
        <property type="entry name" value="SMC_prok"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02169">
        <property type="entry name" value="SMC_prok_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02168">
        <property type="entry name" value="SMC_prok_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43977">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="7">
        <location>
            <begin position="520"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="169"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="660"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="1188" mass="135271" checksum="74A3CDA3F19E2333" modified="2017-09-27" version="1">MPYIEKIEMKGFKSYGNRKVVVPLSKGFTAIVGANGSGKSNIGDAVLFVLGGLSAKAMRATRISDLIFAGTKTEPPAKYAEVAMYFNNEDRGFPVDEDEVVIKRRVYPDGRSTYWLNGKRTSRSDILDVLSAAMISPEGYNLVLQGDITKFIKMSPTERRLLIDEISGIAEYDAKKEKALQELKQAEENLARVDLLIREVKAQLDKLEKERNDALRYLDLKDRVEKAKVTLLLGEIRKLQSLIEESNLRDKEIEAEIAAMEARLKDIAREIVAKEKELNAVEKELEEKSEDGILEVTRKISEVQSKIEMARKNIELAHKEIEDSQRRLAKAKEELKKVSEEIEKSKSAINRWSKRREKLKAEIKEKEVVKNELVIKLGEIDRDFAVAKEEFDKVVDELEEAKKELYMKESDIKKFEEEIERAKAKIAQNNAKKVALKSKIGEARKSLESKRSELGDIDGKISKAEARLRKAEKELEEKTKALRKTESELAKAKEELIKAEAQREVRGNRAVDFLKGQNIPGLYGTLGELITVADEKYALAVEVALGGNYDNVVVEDDRVAEKAIKLLKEKKLGRLTFLPLNKIKPRSMREKPPLGVPAIDVVHYDPRFKNAVAYALGDTLIVEDMDEARTVGIGKVRMVTLGGELLERSGAITGGHYRPRGKLGVNVDEIRKRVEKLEREKEALESAVNALKLEVRGLENELFNLRMKKSELSKDLQVVQNEMDRLLAEDKALAEEIEENERLIEALEKKIHDTKGEMAKLRGRIERLEKKREKLKRALENPEARELNQKIREVEAEISKLREELGKVESKLEGLEVRINEELLPRKADLEEEIEGLVNRINALRANIGENEKAIKDFEAELEELRKAEENVKDELKGLRERRERLKNEIIDLRSEKDELSSKLQELRIEANTLKIKLAQYEATLKEKQDELKHFDPKLIKSIKEIPLELEALREQIEKMEEEIRSLEPVNMKAIEDFEVVERRYLELKSKREQVVAEKESIEEFIEEIEGQKRNVFMQTLNEIAKNFSELFAKLSPGGSARLILENPEDPFAGGLEIEAKPAGKDVKRIEAMSGGEKALTALAFVFAIQRYKPAPFYLFDEIDAHLDDANVKRVADLIKEASQNSQFIVITLRDVMMANADKIIGVSMRKGVSRVVALSLEKAMKILEEARKKSEAEHAEMFGHLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9L2</accession>
    <name>A0A218P9L2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_09040</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIMIBI class G3E GTPase family. ArgK/MeaB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854697.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9L2"/>
    <dbReference type="GeneID" id="33316414"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09040"/>
    <dbReference type="OrthoDB" id="21324at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03114">
        <property type="entry name" value="MMAA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052040">
        <property type="entry name" value="GTPase/Isobutyryl-CoA_mutase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005129">
        <property type="entry name" value="GTPase_ArgK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00750">
        <property type="entry name" value="lao"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43087:SF1">
        <property type="entry name" value="LAO_AO TRANSPORT SYSTEM ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43087">
        <property type="entry name" value="LYSINE/ARGININE/ORNITHINE TRANSPORT SYSTEM KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03308">
        <property type="entry name" value="MeaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="44"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="319" mass="35101" checksum="299345FAD70CF8F3" modified="2017-09-27" version="1">MIDDLIERMLRGDKRAAARLITLVENDEEKAREIISKVYPHTGNAYIVGITGPPGAGKSTLLDKLIKVVREEGKVVGVIAIDPTSPFTGGALLGDRIRMQRHSTDPGVFIRSMATRGSLGGLAKATNDAIKVLDAYGCDVIFVETVGVGQIEIDIVKTADTVVLVTVPGLGDDIQAIKAGLMEIADVFVINKADKEGADATYFELNLMLDLEKERWEKRGWRPPIIETVATTMRGIRDLWKAINDHRKSLEESGRLAEKRKFRAEEEVKTIVSGRIARAVEERLDDEEVSSLIERVVKREIDPYSAADTVLEKALGVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9M8</accession>
    <name>A0A218P9M8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854730.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9M8"/>
    <dbReference type="GeneID" id="33316454"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09240"/>
    <dbReference type="OrthoDB" id="89671at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="76" mass="8522" checksum="4D44F3894159A7B6" modified="2017-09-27" version="1">MIRDFSPSLLPHTLRYVRFNLGASSFKLTGKNGRSRFIVAVENNIVRGVEYESESESLITEVDKNVLEVGEDEFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9T1</accession>
    <name>A0A218P9T1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09515</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854782.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9T1"/>
    <dbReference type="GeneID" id="33316509"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09515"/>
    <dbReference type="OrthoDB" id="98753at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="701"/>
            <end position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="725" mass="78759" checksum="4F019148A7C2C130" modified="2017-09-27" version="1">MNRVGRPEFLVLMLILISLSSEAMALSVEMGLDEVLLVDGQVVTFDVANGGSLILLHVDAGNRSYSPVLRFGENLTVDGVTYAVGSFDPSTLRLRLHVSGNYSSVRVMKKHDFDVKVIEAFDTYVKLNVTSNGFYPLNGTLKVYSGGILLKEVPIELDCGESILLKLSPPSSPLTLVMEEQGISKTVSITKFDKQVSIERIWSNGSPVVELFNRGDPVNATVKLLYNGLTAETKRVELRRGSNTVAFSSPVAQGMVLVDYGALAQESFYLKPALLVLDGVQKNRSVLVVRLRNAGEVTFRGTVSALQNGVIVGNPLSRDVEIKPGELVEVEFEVPSDASYLTLTATSEIGGFSFPVMLGSEGIQVEAQSGSIRVPLGGRGAYILMLKGSGRVRLGIEGLPESMHYSFYAGESEIRELNVDGAAQVSLVLTVPNLPSGFSLGEPITFNVTVYTDDGTRVLPLELEVYGLGLLPVYGDNWLAKMNYTAETHFVGIPYRFAGADLTPPLAFEEWPGEKIAIAYGEYVQQGADLTVHVLSKSGGILHSSEQRKGMADYVVFNETDFMLMLEGDRFRGILLVADYMNGPGNLSFEVSPKEFGTGIRTFIINATGIRGKRLNLKVHSDSPVEIRAYYFTLNDERKDFDPLSSDFKGVFRGRGTSVEGSVNIRPYEDFIAVSIVGAGNVSIEMVPESVPVQAGGLGKYAGYLLIAGLLVLLGLFVYLERRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9W0</accession>
    <name>A0A218P9W0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transglutaminase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_09470</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0252 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_157721617.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9W0"/>
    <dbReference type="GeneID" id="33316500"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09470"/>
    <dbReference type="OrthoDB" id="97757at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.620.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007562">
        <property type="entry name" value="Transglutaminase-like_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04473">
        <property type="entry name" value="DUF553"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="234"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Transglutaminase-like" evidence="3">
        <location>
            <begin position="87"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="253" mass="28908" checksum="17AF5E301F1E44A3" modified="2017-09-27" version="1">MGVLLKGIIKLIIVVLIISAIIYDHQTGVITVKTSDLLQKAGINTIDRDAYLKKYSEAFDCSKPYWRNWGPGIIKCDINESEINAIMPVAEKVKGRDILETAWNLHKWEVENTDYDYEKFMALEGGTLLPHEFLQKKKGVCRDYAVFNYAVMKALGYRDIYLAYISMSSSIWGHAVLVFKYNGTYYALDWWGPQKIDAHYAERWEGSLSIVILNDKGEVVSYVGVTPWEVINRLPQLTVMVIGILLIVLVLKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9W2</accession>
    <name>A0A218P9W2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Archaetidylinositol phosphate synthase</fullName>
            <shortName evidence="2">AIP synthase</shortName>
            <ecNumber evidence="2">2.7.8.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_09710</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the formation of archaetidylinositol phosphate (AIP) from CDP-archaeol (CDP-ArOH or CDP-2,3-bis-(O-phytanyl)-sn-glycerol) and 1L-myo-inositol 1-phosphate (IP or 1D-myo-inositol 3-phosphate). AIP is a precursor of archaetidyl-myo-inositol (AI), an ether-type inositol phospholipid ubiquitously distributed in archaea membranes and essential for glycolipid biosynthesis in archaea.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>CDP-2,3-bis-O-(phytanyl)-sn-glycerol + 1D-myo-inositol 3-phosphate = saturated 1-archaetidyl-1D-myo-inositol 3-phosphate + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36823"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:74004"/>
            <dbReference type="ChEBI" id="CHEBI:74006"/>
            <dbReference type="EC" id="2.7.8.39"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 Mg(2+) or Mn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.39" evidence="2"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854819.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9W2"/>
    <dbReference type="GeneID" id="33316548"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09710"/>
    <dbReference type="OrthoDB" id="9904at2157"/>
    <dbReference type="UniPathway" id="UPA00085"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02242">
        <property type="entry name" value="AIP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044270">
        <property type="entry name" value="AIP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054868">
        <property type="entry name" value="archin_ph_syn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040950">
        <property type="entry name" value="archin_ph_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2 3" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="148"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="187" mass="20473" checksum="E905CE9940F46AF6" modified="2017-09-27" version="1">MVLNNYRENVRGYLEAIVRPLAKAGVTPNTITVLGLLISLAGAYLFYLGEQFWAAIVLLIGSLIDALDGTLARMTGKTSRFGAFLDSTFDRISDGAVLFGIALGGLADWRIAFLTFMGAYLVSYERCRAELAGSGKLAVGIAERAERLLILIGFALFGYVEYGVYLVGILAWVTVLQRMWAAYQRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Z3</accession>
    <name>A0A218P9Z3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Molybdate/tungstate import ATP-binding protein WtpC</fullName>
            <ecNumber evidence="11">7.3.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">A3L08_09780</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Part of the ABC transporter complex WtpABC involved in molybdate/tungstate import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>tungstate(in) + ATP + H2O = tungstate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35027"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46502"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">The complex is composed of two ATP-binding proteins (WtpC), two transmembrane proteins (WtpB) and a solute-binding protein (WtpA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ABC transporter superfamily. Sulfate/tungstate importer (TC 3.A.1.6) family.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.6" evidence="11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854831.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Z3"/>
    <dbReference type="GeneID" id="33316562"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09780"/>
    <dbReference type="OrthoDB" id="18368at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055052">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901238">
        <property type="term" value="F:ABC-type tungstate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015689">
        <property type="term" value="P:molybdate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03299">
        <property type="entry name" value="ABC_ModC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000425">
        <property type="entry name" value="Probable ABC transporter, ATP-binding subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047641">
        <property type="entry name" value="ABC_transpr_MalK/UgpC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053428">
        <property type="entry name" value="Molybdate/tungstate_ABC-ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004606">
        <property type="entry name" value="Mop_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005116">
        <property type="entry name" value="Transp-assoc_OB_typ1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00638">
        <property type="entry name" value="Mop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040840">
        <property type="entry name" value="tungstate_WtpC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43875">
        <property type="entry name" value="MALTODEXTRIN IMPORT ATP-BINDING PROTEIN MSMX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43875:SF15">
        <property type="entry name" value="TREHALOSE IMPORT ATP-BINDING PROTEIN SUGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03459">
        <property type="entry name" value="TOBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="MOP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51866">
        <property type="entry name" value="MOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="2"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Mop" evidence="16">
        <location>
            <begin position="280"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00057369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ASJ07588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="344" mass="38328" checksum="FB647B725AC6B264" modified="2017-09-27" version="1">MLEVNSVSKDYKEFKLRDITFDVREGEHFIILGPSGAGKTVLLEVIAGIIEPDSGRVYLNGEDVTDLPPEKRGLAYIPQNYALFPHMSVFDNIAFGLRLRKLPKAEVERKVREIAEVLDIGHLLGRKPKTLSGGESQRVAIARALVVEPPLILMDEPFANLDVQTRSRLINEMKRWRKELGFTALHVTHSFEEAVSLGDRVGVMLNGRLVQVGSVREVFSRPASEDVARFLGFENIIEGTANGRVLRANGVEIELPVEVHGRIRVGLRPEDIILSLEPLRSSARNEFKAVVDSVEELGPLVRVHLFVGGLHLRAFITRSSMLEMGIRPGKEVYASFKASALHVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA15</accession>
    <name>A0A218PA15_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Septum site-determining protein MinD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA15"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01495"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051782">
        <property type="term" value="P:negative regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050625">
        <property type="entry name" value="ParA/MinD_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384:SF10">
        <property type="entry name" value="ATPASE INVOLVED IN CHROMOSOME PARTITIONING, PARA_MIND FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384">
        <property type="entry name" value="SEPTUM SITE-DETERMINING PROTEIN MIND HOMOLOG, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="1">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="261" mass="29595" checksum="A6A6C02EFA764F1E" modified="2017-09-27" version="1">MTANLGTYFSKVGYRSLVIDGDLYLPKLAFHFGIYTPQYNIHTLLKNPDMRVVEAVYHDPRTGVDILPGSSKLYDIIDLDQKRMRDIVREIQTRYNVTIIDSPVGIPFDTISTFRLAQYQLILVEIERCPIHSLEHMVENEVIKLKSIGDTYGLKVGVVLNKVRESAESVNDIVDFLEYSVEVPVVGVVPFDNRVPEATNYGRPVLDYAPRSGASKAISEAGGVLDEWMFGKKKKRNPLTRLYESLLSLFRRTKAPSLKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA20</accession>
    <name>A0A218PA20_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Polymerase nucleotidyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA20"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03960"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05403">
        <property type="entry name" value="NT_KNTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052548">
        <property type="entry name" value="Type_VII_TA_antitoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33933">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33933:SF3">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE MJ0604-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="92" mass="10583" checksum="6E98361BA8CF4AF7" modified="2017-09-27" version="1">MRERFGDSIEEVILFGSYVRGDYDEESDIDVLIVGEVDFDELMEIVTDILLKHGELVSPIVIKPEEFKRRKDSFIRTVLEEGKPLYSSTSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA66</accession>
    <name>A0A218PA66_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Endonuclease V</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_06655</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage at apurinic or apyrimidinic sites to products with a 5'-phosphate.</text>
            <dbReference type="EC" id="3.1.21.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA66"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06655"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043737">
        <property type="term" value="F:deoxyribonuclease V activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016891">
        <property type="term" value="F:RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06559">
        <property type="entry name" value="Endonuclease_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2170.10">
        <property type="entry name" value="archaeoglobus fulgidus dsm 4304 superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007581">
        <property type="entry name" value="Endonuclease-V"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28511">
        <property type="entry name" value="ENDONUCLEASE V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28511:SF1">
        <property type="entry name" value="ENDONUCLEASE V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04493">
        <property type="entry name" value="Endonuclease_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="188" mass="20628" checksum="50854F28D91BBC09" modified="2017-09-27" version="1">MTERKLELIATLQRKLAGQIVERPLDVSNVKTVAAVDVSYRGDLARAAFVLCSFPECELLRANVVEIEVSVPYIPTYFFLRETRPVLLALRGEDFDVLLVEGHGRAHPRGYGLASHIGLLTGRPTVGVAKRPLRGVPEELFRRTGKAYVSVGNLIDLGSAVGIVEPLLEGGYPKPLKLADRLSKRGEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA90</accession>
    <name>A0A218PA90_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08945</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA90"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08945"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="127" mass="13076" checksum="9559BCE988EB296A" modified="2017-09-27" version="1">MHGAALFAAMGVMGVLSFLIALFIAAIFLSLAGKLVGIEKASIGRSMIAILGGGILGGIVTLLVALVFAPLAPLLGFLANLWVIKTVFETGWLRAFLAWLLSAVMAAVIMMLLAAFGLFTIGALSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P505</accession>
    <name>A0A218P505_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853124.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P505"/>
    <dbReference type="GeneID" id="33314640"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00180"/>
    <dbReference type="OrthoDB" id="56938at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22231">
        <property type="entry name" value="RHH_NikR_HicB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="entry name" value="Met repressor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36215">
        <property type="entry name" value="BLL4998 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36215:SF1">
        <property type="entry name" value="BLL4998 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribbon-helix-helix protein CopG" evidence="1">
        <location>
            <begin position="6"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="63" mass="7417" checksum="53A45E074CDF55B5" modified="2017-09-27" version="1">MAKMRIISVQLPQGLINAMDQLVRKGVYPNRSEIIREAIRELLKKELYQLETEERSTPDYVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P527</accession>
    <name>A0A218P527_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Peptide ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_00295</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853145.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P527"/>
    <dbReference type="GeneID" id="33314663"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00295"/>
    <dbReference type="OrthoDB" id="44105at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045621">
        <property type="entry name" value="BPD_transp_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43163">
        <property type="entry name" value="DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DPPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43163:SF6">
        <property type="entry name" value="DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DPPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19300">
        <property type="entry name" value="BPD_transp_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="301"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="98"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ05882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="333" mass="37493" checksum="52998E614C028ECA" modified="2017-09-27" version="1">MANLKKFLIRRLLTFIPTLIGVTLIVFLIAYVIPADVARAWAGGEKASQAYMEQIRKEYHLDEPWYDQYWFLVSGLAKNDIIDPRTSNYVFDDISERFPVTFELALMAFFFILIIGIPLGIISALKRNTWVDTVIRFFALTGVSMPVFWLGYLLIFVFFVKVHWITLAGFPAPPEHQITHIPIIDALITGDFQTLGQHLHRLWLPGFTLGFMGAGVLARFVRNSFLEAISSDYVAFLKAKGVPKRAIYRHALKNAMVPIVTVLGLQFGGLLGGTPITETVFGLPGMGSYVIESIRNLDFPAVVAITMVFALIFLITNLVVDILYALIDPRVRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P529</accession>
    <name>A0A218P529_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853151.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P529"/>
    <dbReference type="GeneID" id="33314669"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00325"/>
    <dbReference type="OrthoDB" id="97702at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="74" mass="8028" checksum="B5413663CBBE4214" modified="2017-09-27" version="1">MRKQIYIASVVLTAIALFWPILYGNITILMRIPGDPVLQAVAGVLVFGTLAYFTYGEDEGAEGEEAEEEFTASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P576</accession>
    <name>A0A218P576_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase</fullName>
            <ecNumber evidence="9">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">flpA</name>
        <name evidence="10" type="ORF">A3L08_00540</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with nop5. Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA. These sRNP particles form homodimers, giving rise to an asymmetric holoenzyme.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the methyltransferase superfamily. Fibrillarin family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="9"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853191.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P576"/>
    <dbReference type="GeneID" id="33314712"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00540"/>
    <dbReference type="OrthoDB" id="6244at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990259">
        <property type="term" value="F:histone H2AQ104 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008649">
        <property type="term" value="F:rRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000613">
        <property type="entry name" value="Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00351">
        <property type="entry name" value="RNA_methyltransf_FlpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000692">
        <property type="entry name" value="Fibrillarin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020813">
        <property type="entry name" value="Fibrillarin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003276">
        <property type="entry name" value="PRK04266.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003277">
        <property type="entry name" value="PRK04266.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335:SF17">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335">
        <property type="entry name" value="RRNA 2-O-METHYLTRANSFERASE FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01269">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006540">
        <property type="entry name" value="Nop17p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00052">
        <property type="entry name" value="FIBRILLARIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01206">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00566">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 9" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0819">tRNA processing</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="85"/>
            <end position="86"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="104"/>
            <end position="105"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="129"/>
            <end position="130"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="149"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00057806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ05928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="226" mass="25373" checksum="74D26C22BEB381FC" modified="2017-09-27" version="1">MRVKKHKFPGVYVFIDEDGSEKIATKNLVPGQKVYGERLIKAEGEEYRIWNPSRSKLGAAILNGLKNFPIRPGSTVLYLGVASGTTASHVSDIVGWEGKVFGVEFSPRVLRELVPIVEERRNIVPILGDATKPEGYRALVPKVDVIFEDVAQPTQAKILIDNARVYLKSGGYGMISVKSRSIDVTKEPEQVFNEVEKELSEYFEVIERLSLEPYEKDHALFVVRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5A7</accession>
    <name>A0A218P5A7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sarcosine oxidase subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_00685</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853217.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5A7"/>
    <dbReference type="GeneID" id="33314741"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00685"/>
    <dbReference type="OrthoDB" id="27922at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.440">
        <property type="entry name" value="2Fe-2S iron-sulphur cluster binding domain, sarcosine oxidase, alpha subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042204">
        <property type="entry name" value="2Fe-2S-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051691">
        <property type="entry name" value="Metab_Enz_Cyan_OpOx_G3PDH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42949">
        <property type="entry name" value="ANAEROBIC GLYCEROL-3-PHOSPHATE DEHYDROGENASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42949:SF3">
        <property type="entry name" value="ANAEROBIC GLYCEROL-3-PHOSPHATE DEHYDROGENASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13510">
        <property type="entry name" value="Fer2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="2">
        <location>
            <begin position="118"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ05955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="481" mass="52220" checksum="38D4C8073FB11522" modified="2017-09-27" version="1">MRLNEHPLLRFKRGREVTIYFNGQPVKAYEGEPIAAALHAAGIKVLNYSANEKRPRGLFCAIGKCSSCLMIVNGIPNVRTCITLVEGGMRIEPQRGKAKLPKEAKPPEFRDAKAVKADIVVIGGGPAGLMAAIHAADAGASVVLLDENPMLGGQLVKQTHKFFGKREQFAGVRGVEIAKILEKEVRKRDNIEVFLETSAVGIFQEGDEKLVLCVRKNKELMEFRGRALIVATGAMEKMIPFENNDLPGIYGAGAIQTLMNTYGVKPGDRVLIVGAGNVGLILAYQLVQAGVEVEAIVEVMPKVGGYFVHAAKVRRLGIPILTRHTILRAEGKERVERAVIAQLDENWKPIPGTEKVFDVDVIALAVGLRPSIELLHQAGCQIRFVRELGGHVAVRDEWMETTVRGIFVAGDTAGIEEATTAMLEGKIAGIAAALRLGIAEESWVKEIERAQRDLEEFRSGPFGRHVAEGIRKLLAGVGGNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5C5</accession>
    <name>A0A218P5C5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tetratrico peptide repeat group 5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_00850</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853244.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5C5"/>
    <dbReference type="GeneID" id="33314774"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00850"/>
    <dbReference type="OrthoDB" id="97625at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ05982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="18325" checksum="540D3F7E1833D79B" modified="2017-09-27" version="1">MSGVRERALSLFREALEAENARNLEEAKRKLDDIMRLTRKDEPELYFEACFRMADVFIQEDNYRGAVKFAIRGIYLAPSEELRKFGIRRLGDILTILKSENRLQELAENMEPILTLVRDDEELHAFVTALVKLARGEEVKTEIFSDEFERILEELKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5E4</accession>
    <name>A0A218P5E4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YbaK/aminoacyl-tRNA synthetase-associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5E4"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01045"/>
    <dbReference type="OrthoDB" id="27691at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.960.10">
        <property type="entry name" value="YbaK/aminoacyl-tRNA synthetase-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036754">
        <property type="entry name" value="YbaK/aa-tRNA-synt-asso_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007214">
        <property type="entry name" value="YbaK/aa-tRNA-synth-assoc-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30411">
        <property type="entry name" value="CYTOPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30411:SF1">
        <property type="entry name" value="CYTOPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04073">
        <property type="entry name" value="tRNA_edit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55826">
        <property type="entry name" value="YbaK/ProRS associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YbaK/aminoacyl-tRNA synthetase-associated" evidence="1">
        <location>
            <begin position="21"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="145" mass="15947" checksum="A4198CE5B19DB3EB" modified="2017-09-27" version="1">MGKLEEIAKELGAEILEVGRPVKTVEQATRETGTSPKQVIKSLVIISEREPLLVIVDGESRVDMGKLERLFGKCRFAKPEEVKELTGYEVGGVPPVGVPLRTIVDPKVLENEYVIGGGGAIDRLLRIEPQKIVEYQGAEVVDVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5E8</accession>
    <name>A0A218P5E8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9 10">DNA repair and recombination protein RadB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">radB</name>
        <name evidence="12" type="ORF">A3L08_00945</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Involved in DNA repair and in homologous recombination. May regulate the cleavage reactions of the branch-structured DNA. Has a very weak ATPase activity that is not stimulated by DNA. Binds DNA but does not promote DNA strands exchange.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the eukaryotic RecA-like protein family. RadB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853259.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5E8"/>
    <dbReference type="GeneID" id="33314793"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00945"/>
    <dbReference type="OrthoDB" id="17644at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00350">
        <property type="entry name" value="RadB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011939">
        <property type="entry name" value="DNA_repair_and_recomb_RadB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02237">
        <property type="entry name" value="recomb_radB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942:SF47">
        <property type="entry name" value="DNA REPAIR AND RECOMBINATION PROTEIN RADB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942">
        <property type="entry name" value="RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003336">
        <property type="entry name" value="RadB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01874">
        <property type="entry name" value="DNAREPAIRADA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="11">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="NCBIfam" id="TIGR02237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ05997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="234" mass="25733" checksum="F1EEDF51A936A1B4" modified="2017-09-27" version="1">MLSTGVKSLDELLGGGIAGGVLTQVYGSFATGKTTLAVQIGLLSGKKVAYVDTEGGFSPERLSQMAGARGFNPEEALQRFILFTPGDFKEQRRVIGSLKKVVDKTFSLVVVDSLTAHYRAEEPRKNLTNELAKQLQVLLWIARKNEVPVLVINQVHFDSRAGKIKPVAEHTLNYRTKDILRLDKLSTPGLRVAVLERHRFKPEGGMVFFRITERGIEEALGVGEAGDITSDGYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5F1</accession>
    <name>A0A218P5F1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dephospho-CoA kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853288.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5F1"/>
    <dbReference type="GeneID" id="33314824"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01100"/>
    <dbReference type="OrthoDB" id="85381at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006226">
        <property type="entry name" value="PRK08356.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41930:SF1">
        <property type="entry name" value="DEPHOSPHO-COA KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41930">
        <property type="entry name" value="UPF0200 PROTEIN MJ1399"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13238">
        <property type="entry name" value="AAA_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="21980" checksum="DF6ECBF515B3FD5C" modified="2017-09-27" version="1">MIIGVVGKIAAGKTTVAKFFEEKGFCRVSCSDPLIDLLTHNVSDYSWIPELPEKAEPTRDRLIEFGKYLKDKYGGDILIKLAVDKKRHCRNIVIDGVRSKEEIEAIKRMGGKVIYVEARPEIRFERLMRRKASKDKTIKSFEDFKAMDDAEERLYHTSELKDLADYIIVNEGTLEELRKKVKGIIDDIAGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5M3</accession>
    <name>A0A218P5M3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6 7">Acylphosphatase</fullName>
            <ecNumber evidence="2 6 7">3.6.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_01390</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6 7">
            <text>an acyl phosphate + H2O = a carboxylate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="3.6.1.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the acylphosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.7" evidence="2 6 7"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853340.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5M3"/>
    <dbReference type="GeneID" id="33314883"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01390"/>
    <dbReference type="OrthoDB" id="6643at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003998">
        <property type="term" value="F:acylphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000012">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020456">
        <property type="entry name" value="Acylphosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001792">
        <property type="entry name" value="Acylphosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036046">
        <property type="entry name" value="Acylphosphatase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017968">
        <property type="entry name" value="Acylphosphatase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011010">
        <property type="entry name" value="PRK14436.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47268">
        <property type="entry name" value="ACYLPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47268:SF4">
        <property type="entry name" value="ACYLPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00708">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00112">
        <property type="entry name" value="ACYLPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54975">
        <property type="entry name" value="Acylphosphatase/BLUF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00150">
        <property type="entry name" value="ACYLPHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00151">
        <property type="entry name" value="ACYLPHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51160">
        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acylphosphatase-like" evidence="9">
        <location>
            <begin position="5"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="20"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="91" mass="10299" checksum="31551780CE5394E1" modified="2017-09-27" version="1">MAWIRAHLRIHGRVQGVGFRWSMQREARKLGLAGWVRNLPDGTVEAVVEGEEERVEALIGWAHQGPPMARVTKVEVKWEEPEGLEGFRVTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5P5</accession>
    <name>A0A218P5P5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">AsnC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012572508.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5P5"/>
    <dbReference type="GeneID" id="74506649"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01680"/>
    <dbReference type="OrthoDB" id="8136at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.920:FF:000019">
        <property type="entry name" value="Transcriptional regulatory protein, asnC family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF6">
        <property type="entry name" value="REGULATORY PROTEIN ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="1">
        <location>
            <begin position="8"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="75" mass="8578" checksum="67F8DAC2C1A54FC1" modified="2017-09-27" version="1">MVTAFILMVTAAGKEREVMEKLLAMPEVKEAYVVYGEYDLVVKVETDTLKDLDQFITEKIRKMSEIQMTSTMIAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5S1</accession>
    <name>A0A218P5S1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.282</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">tRNA wyosine derivatives biosynthesis protein Taw3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">taw3</name>
        <name evidence="9" type="ORF">A3L08_01630</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wyosine derivatives biosynthesis pathway. Probably methylates N-4 position of wybutosine-86 to produce wybutosine-72.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36635"/>
            <dbReference type="Rhea" id="RHEA-COMP:10378"/>
            <dbReference type="Rhea" id="RHEA-COMP:10379"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73543"/>
            <dbReference type="ChEBI" id="CHEBI:73550"/>
            <dbReference type="EC" id="2.1.1.282"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the TYW3 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.282" evidence="7"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853382.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5S1"/>
    <dbReference type="GeneID" id="33314931"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01630"/>
    <dbReference type="OrthoDB" id="19299at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1960.10:FF:000010">
        <property type="entry name" value="tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1960.10">
        <property type="entry name" value="tRNA wybutosine-synthesizing-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00266">
        <property type="entry name" value="TYW3_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022908">
        <property type="entry name" value="Taw3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003827">
        <property type="entry name" value="tRNA_yW-synthesising"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036602">
        <property type="entry name" value="tRNA_yW-synthesising-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003264">
        <property type="entry name" value="PRK04235.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047731">
        <property type="entry name" value="tRNAMtaseTaw3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48418">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48418:SF1">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02676">
        <property type="entry name" value="TYW3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111278">
        <property type="entry name" value="SSo0622-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA wybutosine-synthesizing protein" evidence="8">
        <location>
            <begin position="3"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="212" mass="24346" checksum="0D5E6E613F6AC92B" modified="2017-09-27" version="1">MKAKREALTSLFTAMREGKVDEDIIELLLLINSIKGVYTTSSCSGRIGIIEEPALGAKPLSRWLIKVHRPIEFEEALDALRNAREGLIFLKSQPPIFHIVAEDMENAKKLHEIGLASGFKYTTFKVISNRYLVEINATEYLTTPLGKDGKVLVDEAYLRFAIEVGNSMLKRSKGRLTRLEENFRKLREELGEDELFYELVRDFEIEKNWRIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5U7</accession>
    <name>A0A218P5U7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rubrerythrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01815</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5U7"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01815"/>
    <dbReference type="OrthoDB" id="102142at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01045">
        <property type="entry name" value="Ferritin_like_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rr_diiron-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33531:SF10">
        <property type="entry name" value="BLR7895 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33531">
        <property type="entry name" value="RUBRERYTHRIN SUBFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rubrerythrin diiron-binding" evidence="1">
        <location>
            <begin position="18"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="159" mass="18676" checksum="43B5E8F9338C7398" modified="2017-09-27" version="1">MIDVEEIIERLSKLSYEEALAYWIKSEREEAEFYRQLAERAINLGLPESLVETFKKLSKDSEKHAKELTKLFRESYSKEPESDIPPIEVLSILEEFERADQVGEVLKAAMESELIAMNAYRTLAEKVEDPKLRELYLKLSEVERTHYEALKREYEKLGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5U9</accession>
    <name>A0A218P5U9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Membrane dipeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01655</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853386.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5U9"/>
    <dbReference type="GeneID" id="33314936"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01655"/>
    <dbReference type="OrthoDB" id="26221at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070573">
        <property type="term" value="F:metallodipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01301">
        <property type="entry name" value="rDP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000180">
        <property type="entry name" value="Dipep_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008257">
        <property type="entry name" value="Pept_M19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10443:SF12">
        <property type="entry name" value="DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10443">
        <property type="entry name" value="MICROSOMAL DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01244">
        <property type="entry name" value="Peptidase_M19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00869">
        <property type="entry name" value="RENAL_DIPEPTIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51365">
        <property type="entry name" value="RENAL_DIPEPTIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="311" mass="35338" checksum="4AC76525941968AF" modified="2017-09-27" version="1">MIFDAHSDLPTFVYDERKRGKTTVLERNFERFFSGWVSSRVMAIWTRPERRGDATIYGLEVLNTLLKDVEESERFELVKSVGEMREAIEEGRVALWLSLEGGEPIEESLDLLEVFYRLGLRVLTLTWSLRNAIADGIFERTNGGLTNFGVEVVGKAEELGIVIDLSHINEAGFWDTLDVTAFPVIASHSNAKKLCDNPRNLTDDQILAIAERSGVIGAVAIPSFVDREMPTLEKYVEHIAYMVDLAGYKHVGLGFDFVYYLPGWSGRSVEGFEDESRIPALLERLRENFSEKEVEAITFRNFERIFERVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Z1</accession>
    <name>A0A218P5Z1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Macro domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853462.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5Z1"/>
    <dbReference type="GeneID" id="33315018"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02065"/>
    <dbReference type="OrthoDB" id="15450at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001662">
        <property type="entry name" value="PRK00431.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106">
        <property type="entry name" value="GANGLIOSIDE INDUCED DIFFERENTIATION ASSOCIATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106:SF27">
        <property type="entry name" value="MACRO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="1">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="179" mass="19840" checksum="8A7B7A6ADC8E2CE0" modified="2017-09-27" version="1">MRFEIVRGDITRFPAEAIVNAANRYLEHGGGVAYAIAKAAAGDVREYIRISKEAMREQLGKSSIEHGEVVVTPAIRLEQYGIKYVIHTVGPYCGGVWDEDKKEKLKKAILGALRKAEELGIRSIAFPAVSAGIYGCPLEEVVKTFKEVVGEFGKEEGSVERVYLVLYSKEDYERALKVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P647</accession>
    <name>A0A218P647_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Sodium:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">A3L08_02380</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853518.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P647"/>
    <dbReference type="GeneID" id="33315081"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02380"/>
    <dbReference type="OrthoDB" id="12029at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562">
        <property type="entry name" value="NAPA-TYPE SODIUM/HYDROGEN ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562:SF3">
        <property type="entry name" value="SODIUM ION_PROTON EXCHANGER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="144"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="174"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="298"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="334"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="362"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="11">
        <location>
            <begin position="14"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="391" mass="42144" checksum="FB2EFB7D5D13569A" modified="2017-09-27" version="1">MDFLAALAILLVTAKSIEWLFERVEIHPIIAHVLTGILLGPFVLGIIKPTEELKVLAEFGLIMMMLYMGLTSNFSAIAQNTKKATVVAALGVAFSFALGFLTVEFFGKGTTAAIFIGITLGNTAIEVTSGVLVKEHVKRTVSSILMGAAFADDIMAVYLIGIITALAGGGLNPISFGILTVKIFAFIAATLLVSEYVFKRARWFYSVVRNLNVFFTFTLILTFTLAIIAQWAGLNQIIGAYLAGLTISRLRERKDPLVVTRIKLNELIEDLQVVLTEFFIPLFFIYVGLMFNPPLADISLALIGALYLAAILGKLLGCGLGAKLFGLSWRDSALIGIGMGGRGSLELAILTFGLNAGLIDQALFASVIAVSMLTALTTPIFFRAYIKRAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P664</accession>
    <name>A0A218P664_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_02485</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853537.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P664"/>
    <dbReference type="GeneID" id="33315102"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02485"/>
    <dbReference type="OrthoDB" id="87732at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025302">
        <property type="entry name" value="DrrA1/2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43335">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43335:SF4">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13732">
        <property type="entry name" value="DrrA1-3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="2"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="305" mass="34293" checksum="A28468854C27D193" modified="2017-09-27" version="1">MLRIEKLVKVYKDVRALDGLSLEVKPGQVYGFLGPNGAGKSTTILSTLGLIFPQEGRIELFGEEVFRDGKFDEGRLVKVKARIGYMPEHATLWDFLTPVQTLDTIADAFKIPKAEKEKRINELLDLVGLKDARNKKVGKFSKGMRQRLLLAQALINDPELLILDEPMSGLDPKGIAEFKDIIREQKKAGKTVFFSSHILAHVEEVCDTVGVIVKGKLRLEDSIENIKKEFLKKAGYTILIETNKPVDWRSAELSVTSLGENKYRVVASRDVREEIHDYVASQGAKVLTMQVKEPSLEEIFLKLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P665</accession>
    <name>A0A218P665_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein-export membrane protein SecD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">secD</name>
        <name evidence="11" type="ORF">A3L08_02560</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in protein export.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Part of the protein translocation apparatus. Forms a complex with SecF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SecD/SecF family. SecD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853551.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P665"/>
    <dbReference type="GeneID" id="33315118"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02560"/>
    <dbReference type="OrthoDB" id="146638at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3400">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01463_A">
        <property type="entry name" value="SecD_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022813">
        <property type="entry name" value="SecD/SecF_arch_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024912">
        <property type="entry name" value="SecD_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048634">
        <property type="entry name" value="SecD_SecF_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006216">
        <property type="entry name" value="PRK08343.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081:SF1">
        <property type="entry name" value="PROTEIN TRANSLOCASE SUBUNIT SECD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081">
        <property type="entry name" value="PROTEIN-EXPORT MEMBRANE PROTEIN SEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02355">
        <property type="entry name" value="SecD_SecF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="350"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="374"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="448"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="477"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="Protein export membrane protein SecD/SecF C-terminal" evidence="10">
        <location>
            <begin position="330"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="507" mass="55496" checksum="509BB99208BCD65D" modified="2017-09-27" version="1">MKKRTKKLLFNWRIILLILFLVGSIATLALKPLTFGIDISGGVALVAQTEHPVDSDTMQLVVDSLQKRLNTLGVRDITIEAQGNQIVLVKVANVSTEEQANQIKAVIENQGVFYMEFNGEIFGTGKDVEYVGIYQIKSDNSWAVPFRISKAAAEKFAKLAEGKPGWPVDMFLDPPVNSLMVVPDNIYRLMNSTEFNAQAPNAPTLLERITKAFNITVVTYANQSAEEIAKLAQGKDKIVLINVPQELQTELENMNLTVRYIPGEPGVSDRQIIIKALGLYGPYSLGEGLASGQPQQDVQITGSAPDRLTAEREASTIYTVLKSGSLPVKLNVVGMEFISPRLGENFKTQALYAGLAALMTVLIIIYYHYRNWKIAIPVASTSLFEVIIILGFAALINWNLDLPSIAGIIAAIGTGVDQQVVITDELLSGERSERITRRASVLKRMGRAFFVIFASAATTIAAMSFLLVYFVGTLKGFAFTTILGVLIGILITRPAYAEIAKYLLGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P682</accession>
    <name>A0A218P682_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Peptide ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_02660</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853571.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P682"/>
    <dbReference type="GeneID" id="33315138"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02660"/>
    <dbReference type="OrthoDB" id="44105at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30465:SF45">
        <property type="entry name" value="BINDING-PROTEIN-DEPENDENT TRANSPORT SYSTEMS INNER MEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30465">
        <property type="entry name" value="INNER MEMBRANE ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="160"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="271"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="313"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="123"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="351" mass="39502" checksum="90BA1D4457940F8F" modified="2017-09-27" version="1">MGYLRYLLFRIANAILVLLIVTFIIAALFVKVAEESNRARMNDEMMQWDRTTGQKILKTGGQDAYEKAKAEQEKLLREKYELDLPYWQKVYKKALRTLRLNFGTTTTPIFGTNNVAEIIKVAVPRSILLFTTATIIVIILGIFLGVRAANNPGSVFDRGLSIFALLTYSLPMWWTGMMFLLLFAYKLGWFPLSSMFDPNLTGWAHVKDVLWKLTLPVLTYVFVAFGGWAWTTRNIMIGTLQEDFIMAARAKGVPERKVIYGHALRAAAPPIVTMVIFALLGSLGGAIISELVFNYPGMGRLYWVALQQNETNLLIGLTYFFTMLYLAGVVLADMVYGFLDPRVKVGASANM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6F3</accession>
    <name>A0A218P6F3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">UDP-glucose 4-epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_02965</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853627.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6F3"/>
    <dbReference type="GeneID" id="33315199"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02965"/>
    <dbReference type="OrthoDB" id="4907at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048040">
        <property type="term" value="F:UDP-glucuronate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042732">
        <property type="term" value="P:D-xylose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05234">
        <property type="entry name" value="UDP_G4E_2_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000913">
        <property type="entry name" value="UDP-glucose 4-epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044516">
        <property type="entry name" value="UXS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43078:SF6">
        <property type="entry name" value="UDP-GLUCURONIC ACID DECARBOXYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43078">
        <property type="entry name" value="UDP-GLUCURONIC ACID DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="5">
        <location>
            <begin position="3"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="317" mass="35662" checksum="15CC47F8099A7F04" modified="2017-09-27" version="1">MRVLVTGGAGFIGSHLVDALMESRNEVRVLDDLSAGSLDNIKRWLDNERFEFIKGDMRNPEIVERAVEGVDVVFHLAANPEVRIGAQSPELLYETNVLITYNLLNAMRDSDVEYLVFTSSSTVYGDAEVIPTLEDYAPLEPISVYGGAKLAAEALISGYAHTFGFRALIFRLANIIGERSNHGVIYDFINKLKKNPDELEILGDGTQRKSYLHVSDTVEGMLHIFEHFRKESKTVDFYNMGNDDWITVKEIAEIVSEEMGLNPRFRFTGGVDGGRGWKGDVKFMRLSIEKAKKTGWRPKMNSYEAVRRTVRELLGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6G8</accession>
    <name>A0A218P6G8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Exosortase/archaeosortase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_03005</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853634.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6G8"/>
    <dbReference type="GeneID" id="33315207"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03005"/>
    <dbReference type="OrthoDB" id="102280at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026392">
        <property type="entry name" value="Exo/Archaeosortase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019127">
        <property type="entry name" value="Exosortase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04178">
        <property type="entry name" value="exo_archaeo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09721">
        <property type="entry name" value="Exosortase_EpsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="271" mass="30617" checksum="8FD3C126C4FEBA00" modified="2017-09-27" version="1">MRRSELYIAFSFLFTVLLFALFRTPFLLYFGLVYSLVLYLRRFALVPRAFKFDVGVILGIALMAISPVFLVVRFGEYTSPSTFHALFLLGLFSVFFEVRDGFLPLIPLAEAMIALIAKTDTFEVAVNWLTHLFMDITSYLVRGLIDIFNLPIAMKGNVAVVRNSMVIIGSGCSGLDAFILYILAALLLIYLRKSSRREAALLLVGALGIIPLNAVRIFTLLVIGYHSGISFLELFHSHLGDLMFVAYVFLYWWAVLRWKKQVKGEEKLTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6H4</accession>
    <name>A0A218P6H4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dTDP-4-dehydrorhamnose 3,5-epimerase</fullName>
            <ecNumber evidence="3">5.1.3.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Thymidine diphospho-4-keto-rhamnose 3,5-epimerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_03045</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the epimerization of the C3' and C5'positions of dTDP-6-deoxy-D-xylo-4-hexulose, forming dTDP-6-deoxy-L-lyxo-4-hexulose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-beta-L-rhamnose</text>
            <dbReference type="Rhea" id="RHEA:16969"/>
            <dbReference type="ChEBI" id="CHEBI:57649"/>
            <dbReference type="ChEBI" id="CHEBI:62830"/>
            <dbReference type="EC" id="5.1.3.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the dTDP-4-dehydrorhamnose 3,5-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.13" evidence="3"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853641.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6H4"/>
    <dbReference type="GeneID" id="33315215"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03045"/>
    <dbReference type="OrthoDB" id="2990at2157"/>
    <dbReference type="UniPathway" id="UPA00124"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008830">
        <property type="term" value="F:dTDP-4-dehydrorhamnose 3,5-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019305">
        <property type="term" value="P:dTDP-rhamnose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00438">
        <property type="entry name" value="cupin_RmlC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000888">
        <property type="entry name" value="RmlC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01221">
        <property type="entry name" value="rmlC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21047">
        <property type="entry name" value="DTDP-6-DEOXY-D-GLUCOSE-3,5 EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21047:SF2">
        <property type="entry name" value="THYMIDINE DIPHOSPHO-4-KETO-RHAMNOSE 3,5-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00908">
        <property type="entry name" value="dTDP_sugar_isom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="site" description="Participates in a stacking interaction with the thymidine ring of dTDP-4-oxo-6-deoxyglucose" evidence="2">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR600888-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR600888-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU364069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="166" mass="19274" checksum="A37DD958C7D35EEA" modified="2017-09-27" version="1">MPFEFKRLEIPDVVLIKPRVFEDERGFFMETYKKQDFEKAGIKGEFIQDNHSRSKYGVLRGLHFQREPYAQAKIVRAIRGVIYDVAVDLRKNSPTFGKWVGVILSEYNKWQLYIPRGFAHGFVVLSDVAEVVYKVDNVYAPDYEAGIIWNDPEIGIDWPVDEPVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6I0</accession>
    <name>A0A218P6I0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NAD(P)-dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853644.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6I0"/>
    <dbReference type="GeneID" id="33315218"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03060"/>
    <dbReference type="OrthoDB" id="4907at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008831">
        <property type="term" value="F:dTDP-4-dehydrorhamnose reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019305">
        <property type="term" value="P:dTDP-rhamnose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05254">
        <property type="entry name" value="dTDP_HR_like_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005913">
        <property type="entry name" value="dTDP_dehydrorham_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029903">
        <property type="entry name" value="RmlD-like-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01214">
        <property type="entry name" value="rmlD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10491">
        <property type="entry name" value="DTDP-4-DEHYDRORHAMNOSE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10491:SF4">
        <property type="entry name" value="METHIONINE ADENOSYLTRANSFERASE 2 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04321">
        <property type="entry name" value="RmlD_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RmlD-like substrate binding" evidence="1">
        <location>
            <begin position="1"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="287" mass="32764" checksum="C6B8171AA12FB447" modified="2017-09-27" version="1">MKIAIIGANGQLGTDLVEVFGEDPSFEVIPLTHKDLDVTIPESLRILKELKPDVIVNTAAYVRVDDAELYPEKAFAVNAIGALNVARVSSEIGTINVYISTDYVFDGEKGKPYTEEDVPNPVNVYGASKYAGEIFTRNYSEKYYIIRVASLYGKAGASGKGGNFVEWVIEKAKRGEELKIVNDQFMSPTYTMDVARTLKEFLKLKPEFGIYHMVNEGYCSWYEFTRAIFEILGWEVEVKPIKSSELNRLARRPRLSALENRRLHKIGLRMPNWKERLKRYLVEKRYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6J5</accession>
    <name>A0A218P6J5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Formate-dependent phosphoribosylglycinamide formyltransferase</fullName>
            <ecNumber evidence="8">6.3.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">5'-phosphoribosylglycinamide transformylase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Formate-dependent GAR transformylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">GAR transformylase 2</fullName>
            <shortName evidence="8">GART 2</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Non-folate glycinamide ribonucleotide transformylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Phosphoribosylglycinamide formyltransferase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">purT</name>
        <name evidence="11" type="ORF">A3L08_03245</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the de novo purine biosynthesis. Catalyzes the transfer of formate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR). Formate is provided by PurU via hydrolysis of 10-formyl-tetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(1)-(5-phospho-beta-D-ribosyl)glycinamide + formate + ATP = N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24829"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (formate route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PurK/PurT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.21" evidence="8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853673.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6J5"/>
    <dbReference type="GeneID" id="33315255"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03245"/>
    <dbReference type="OrthoDB" id="9299at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00127"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043815">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase 2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004644">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000013">
        <property type="entry name" value="Formate-dependent phosphoribosylglycinamide formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000035">
        <property type="entry name" value="Formate-dependent phosphoribosylglycinamide formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000022">
        <property type="entry name" value="Formate-dependent phosphoribosylglycinamide formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01643">
        <property type="entry name" value="PurT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003135">
        <property type="entry name" value="ATP-grasp_carboxylate-amine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005862">
        <property type="entry name" value="PurT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054350">
        <property type="entry name" value="PurT/PurK_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048740">
        <property type="entry name" value="PurT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006766">
        <property type="entry name" value="PRK09288.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01142">
        <property type="entry name" value="purT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43055">
        <property type="entry name" value="FORMATE-DEPENDENT PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43055:SF1">
        <property type="entry name" value="FORMATE-DEPENDENT PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02222">
        <property type="entry name" value="ATP-grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21244">
        <property type="entry name" value="PurT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22660">
        <property type="entry name" value="RS_preATP-grasp-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-grasp" evidence="10">
        <location>
            <begin position="123"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="26"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>N(1)-(5-phospho-beta-D-ribosyl)glycinamide</name>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>N(1)-(5-phospho-beta-D-ribosyl)glycinamide</name>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="199"/>
            <end position="202"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>N(1)-(5-phospho-beta-D-ribosyl)glycinamide</name>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="375"/>
        </location>
        <ligand>
            <name>N(1)-(5-phospho-beta-D-ribosyl)glycinamide</name>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="382"/>
            <end position="383"/>
        </location>
        <ligand>
            <name>N(1)-(5-phospho-beta-D-ribosyl)glycinamide</name>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="422" mass="46950" checksum="2629B3337853BB2F" modified="2017-09-27" version="1">MIKPRDELGTAMTDSAQKILLLGSGELGKEIAIEAQRLGVEVIAVDRYANAPAMQIAHRAYVGDMKNADFLFSVVEREKPDAIIPEIEAINLDALFELEKDGYFVVPNAKATWIAMHRERLRETLVKEAKVPTSRYAYATTLDELYDVCEKIGYPCHTKAIMSSSGKGSYFVKGPEDIPKAWEEAKKKARGSADKIIVEEHIEFDVEITELAVRHYDENGDIVTTFPKPVGHYQIDGDYHASWQPAEISERAEREVYRIAKRITDVLGGLGLFGVEMFVKGDKVWANEVSPRPHDTGMVTLASHPTGFSEFGLHLRAVLGLPIPGEWVDGYRLFPMLTPAATHVIKASVSGYSPRFRGLAKALSVPNATIRLFGKPEAYPGRRLGVAIAWDKSVSDAKKRAEMVAHAVELRTRTSTWQRQDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6L8</accession>
    <name>A0A218P6L8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protoporphyrinogen oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_03355</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6L8"/>
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    <dbReference type="KEGG" id="tpaf:A3L08_03355"/>
    <dbReference type="OrthoDB" id="27149at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
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        <property type="term" value="C:eRF1 methyltransferase complex"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:protein methyltransferase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052190">
        <property type="entry name" value="Euk-Arch_PrmC-MTase"/>
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        <property type="entry name" value="PrmC-related"/>
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        <property type="entry name" value="SAM-dependent_MTases_sf"/>
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        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
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        <property type="entry name" value="hemK_rel_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRK14968.1-3"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875:SF1">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase small" evidence="4">
        <location>
            <begin position="22"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="193" mass="21396" checksum="1790AA24D18A3483" modified="2017-09-27" version="1">MPLYYGLNIKLHPQVYEPAEDTFLLAENLAVREGDTALDMGTGTGLIALLMARRAKFVLGVDVNPIAVELARENARLNGIQNVEFFLSDLFENVSGRFDVITFNAPYLPGEPEEPIDLALVGGETGREVLDRFIKEVPDYLKPGGIVQVVQSSITGVEETLKRLEKVGLTARVAAKRHIFFEDILLINAQARW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6N3</accession>
    <name>A0A218P6N3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase</fullName>
            <ecNumber evidence="9">4.1.3.44</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">tRNA wyosine derivatives biosynthesis protein Taw1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">taw1</name>
        <name evidence="11" type="ORF">A3L08_03350</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the wyosine derivatives biosynthesis pathway that catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine (imG-14) on guanosine-37 of tRNA(Phe).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>N(1)-methylguanosine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + 5'-deoxyadenosine + L-methionine + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:36347"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10165"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="EC" id="4.1.3.44"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TYW1 family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.44" evidence="9"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853692.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6N3"/>
    <dbReference type="GeneID" id="33315276"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03350"/>
    <dbReference type="OrthoDB" id="68499at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102521">
        <property type="term" value="F:tRNA-4-demethylwyosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01921">
        <property type="entry name" value="TYW1_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013917">
        <property type="entry name" value="tRNA_wybutosine-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034556">
        <property type="entry name" value="tRNA_wybutosine-synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023993">
        <property type="entry name" value="TYW1_archaea"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03972">
        <property type="entry name" value="rSAM_TYW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930:SF0">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE TYW1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08608">
        <property type="entry name" value="Wyosine_form"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00284">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01071">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Radical SAM core" evidence="10">
        <location>
            <begin position="55"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="333" mass="38838" checksum="E531F896B31FCFF3" modified="2017-09-27" version="1">MAITFVSNPNMPEEMARLFRKQHYALVGRHSSVKLCHWLKESIKHDRFCYKQKFYNIHSHRCLQMTPVTAWCTHNCIFCWRPMEGFLGTELPEPWDDPAFIVEESIKAQRKLLVGYKGMPGINMKKFEEAWNPKHAAISLSGEPMLYPYMGDLVEEFHKRGFTTFIVTNGTVPERLEEMKREDKLPTQLYVSLTAPDIETYNRVNVPMIPDGWDRIKETLALMRDAQTRTVIRLTLVKGENMHNPEGYAKLIKLANPMFVEAKAYMFVGFSRNRLTINNMPRHEEIKAFAEELVKYLPGYHIEDEYEPSRVVLIMRDDVDPHGTGVNGRFIKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6N6</accession>
    <name>A0A218P6N6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerase subunit Rpo7</fullName>
            <ecNumber evidence="4">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">DNA-directed RNA polymerase subunit E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpo7</name>
        <name evidence="4" type="synonym">rpoE</name>
        <name evidence="6" type="ORF">A3L08_03380</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the RNA polymerase complex. Forms a stalk with Rpo4 that extends from the main structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="4">Forms 2 domains with an elongated structure; Rpo4 packs into the hinge region between the 2 domains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="4"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853697.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6N6"/>
    <dbReference type="GeneID" id="33315282"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03380"/>
    <dbReference type="OrthoDB" id="7927at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04331">
        <property type="entry name" value="RNAP_E_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04460">
        <property type="entry name" value="S1_RpoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00865">
        <property type="entry name" value="RNApol_arch_Rpo7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004519">
        <property type="entry name" value="RNAP_E/RPC8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046399">
        <property type="entry name" value="RNApol_Rpo7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005576">
        <property type="entry name" value="Rpb7-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006333">
        <property type="entry name" value="PRK08563.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00448">
        <property type="entry name" value="rpoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF4">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03876">
        <property type="entry name" value="SHS2_Rpb7-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="N-terminal, heterodimerisation domain of RBP7 (RpoE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 6" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S1 motif" evidence="5">
        <location>
            <begin position="82"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="186" mass="21214" checksum="09221FB5342DC03D" modified="2017-09-27" version="1">MYKLLKIKDVVRIPPRMFTMDPKEAAKLVLRETYEGIYDRDEGVVLAVMDVEDIGQGVIVPGDGATYHEVVFDVLVWKPEMHEVVEGEVIDVAPYGAFIRIGPMDGLVHISQLMDDYVVFDEKNKQFLGKETNRTLKLGDYVRARVIAISVKSRVIRENKIGLTMRQPGLGKRDWIEKEKHKEAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6P1</accession>
    <name>A0A218P6P1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1102 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03405</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853702.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6P1"/>
    <dbReference type="GeneID" id="33315287"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03405"/>
    <dbReference type="OrthoDB" id="85476at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009482">
        <property type="entry name" value="DUF1102"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06510">
        <property type="entry name" value="DUF1102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="182" mass="20344" checksum="E798486B8105EBE1" modified="2017-09-27" version="1">MNKLFGLAFLMIGMLLAVGAGANFRYYAADRSASFQVVSDDNELIDLTALQPYVTYDAGKLYVDISQYNPNYNQSRGWGTGMSPNTTYVFEEMFEVSNELWENNQTDYPICVKIKTDHDNVLIFAGNYTNTIAGPDDNIEFTVEHGNPIPIGMIFDNTNATLNMDQFQMSFEAHAGACVPQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6R7</accession>
    <name>A0A218P6R7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_03520</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853721.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6R7"/>
    <dbReference type="GeneID" id="33315309"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03520"/>
    <dbReference type="OrthoDB" id="27015at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030665">
        <property type="entry name" value="KaiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010624">
        <property type="entry name" value="KaiC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637:SF1">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039117">
        <property type="entry name" value="KaiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01874">
        <property type="entry name" value="DNAREPAIRADA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51146">
        <property type="entry name" value="KAIC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="KaiC" evidence="10">
        <location>
            <begin position="2"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="RecA family profile 1" evidence="9">
        <location>
            <begin position="234"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="KaiC" evidence="10">
        <location>
            <begin position="238"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="448" mass="50523" checksum="68BFF4050B057CDB" modified="2017-09-27" version="1">MKIIPTGIESLDRALSGGFNRGSTVLIAGNPGAGKTHLMLHVLYNNMKRGLKGAYISFAETKKQFYEGALNQGIDLREMEEKGLFRFYDMLTLPKDEFEDFVDYLVTDLVDWKPDIVVFDSITVFGQVFGEIHLRAFLHSIVGRLVNALNMLVFLIDEIPYGGKGIGFGIEEFVADGVIVLEMEKYGEIIRRYMRIPKMRNRPLERYSYEYVITDKGIEVFAVPELEFVEEEKRRERIRTNIPGFDDLLGGGLYEGSITLLAGPTGSGKTILALNIAANLSKAGKRVLYIAFEETAEGLRDAKEKLGLEGEFKILSLVPEAKTPVEYYVMIKSLVERENADVLIIDSLSAMQAHMNEEEFTKALRYLQLLSKERALTLVLTYTGAGWEKMVRTGFSTLVDNLVFLMYTFPKAIGETLERAIIVLKSRRSKSDPTLRRFTIGEGGIRIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6U4</accession>
    <name>A0A218P6U4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">L-asparaginase</fullName>
            <ecNumber evidence="2">3.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_03770</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the asparaginase 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.1" evidence="2"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853764.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6U4"/>
    <dbReference type="GeneID" id="33315359"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03770"/>
    <dbReference type="OrthoDB" id="85706at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08963">
        <property type="entry name" value="L-asparaginase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1170:FF:000001">
        <property type="entry name" value="L-asparaginase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="entry name" value="L-asparaginase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006033">
        <property type="entry name" value="AsnA_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020827">
        <property type="entry name" value="Asparaginase/glutaminase_AS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027475">
        <property type="entry name" value="Asparaginase/glutaminase_AS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041725">
        <property type="entry name" value="L-asparaginase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00519">
        <property type="entry name" value="asnASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707:SF28">
        <property type="entry name" value="60 KDA LYSOPHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500176">
        <property type="entry name" value="L_ASNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00139">
        <property type="entry name" value="ASNGLNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00057">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00144">
        <property type="entry name" value="ASN_GLN_ASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00917">
        <property type="entry name" value="ASN_GLN_ASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="L-asparaginase N-terminal" evidence="7">
        <location>
            <begin position="2"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase/glutaminase C-terminal" evidence="8">
        <location>
            <begin position="203"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00070292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="329" mass="35665" checksum="2FB640C5CBD466D9" modified="2017-09-27" version="1">MRILIIGTGGTIASARTEKGYKAALSADEILEVAGIKKRNGLIIETEDILNLDSTLIQPEDWVTIGEAVFRNLSDYDGIVITHGTDTLAYTSSALSFMLRNVPIPVVLTGSMLPITEPKSDAPRNLRTALTFASKGFPGIYVAFMDKIMLGTRVSKVHSLGLNAFQSINYPDIAYVKGGELKVLHRPEVPQGEVIFDPLLDPNVVHIRLTPGLSPEVFSSIGEWSHGIVLEGYGAGGIPYRKRNLLKAVERVAGEKPVVMTTQAVYGGVDLTRYEVGRRALEAGIIPAGDMTKEATLTKLMWALGHTADVAEVREIMERNIAGEITPRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6V3</accession>
    <name>A0A218P6V3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Nucleotide pyrophosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03830</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853776.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6V3"/>
    <dbReference type="GeneID" id="33315371"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03830"/>
    <dbReference type="OrthoDB" id="147562at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047840">
        <property type="term" value="F:dCTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006253">
        <property type="term" value="P:dCTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042262">
        <property type="term" value="P:DNA protection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11537">
        <property type="entry name" value="NTP-PPase_RS21-C6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1080">
        <property type="entry name" value="MazG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052555">
        <property type="entry name" value="dCTP_Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025984">
        <property type="entry name" value="DCTPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46523">
        <property type="entry name" value="DCTP PYROPHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46523:SF1">
        <property type="entry name" value="DCTP PYROPHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12643">
        <property type="entry name" value="MazG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029826">
        <property type="entry name" value="UCP029826_pph"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101386">
        <property type="entry name" value="all-alpha NTP pyrophosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="108" mass="12339" checksum="BF5A2906CFDF1107" modified="2017-09-27" version="1">MDFKELERRLVEFRDARGWRKYHTPKNLAISAAVELGELLEHFQWESDGEILEAVKESGKKEAIADEIADVVIYLTLLAHELGIDLSEALERKIEKNGRKYPVGVTSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6W3</accession>
    <name>A0A218P6W3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03810</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853772.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6W3"/>
    <dbReference type="GeneID" id="33315367"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03810"/>
    <dbReference type="OrthoDB" id="85246at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.440">
        <property type="entry name" value="Protein of unknown function DUF4152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025206">
        <property type="entry name" value="DUF4152"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13680">
        <property type="entry name" value="DUF4152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="227" mass="25468" checksum="C4FDE2E23D20A5D7" modified="2017-09-27" version="1">MKFVAADTGGALLTEDYEPVGLIATAAVLVEKPYRTATLSAVRYADPFNYDMSGRQAVRDEAFLAVELAREVKPDVIHLDSTIGGIEVRKLDEPTIDALTITDRGKEVWKDLAKDLQPLAKKFWEETGIEIIAIGKWSVPVRIAEIYSGIYTAKWAIDYARENGKVMVGLPRYMKVEIKPGQIYGESLDPREGGLFGEIEADTDGIGWELYPNPLVRRYMVLEVWRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P719</accession>
    <name>A0A218P719_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl6</name>
        <name evidence="8" type="ORF">A3L08_04130</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 6">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853830.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P719"/>
    <dbReference type="GeneID" id="33315430"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04130"/>
    <dbReference type="OrthoDB" id="7144at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000008">
        <property type="entry name" value="50S ribosomal protein L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000004">
        <property type="entry name" value="60S ribosomal protein L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="entry name" value="Ribosomal protein L6, alpha-beta domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01365_A">
        <property type="entry name" value="Ribosomal_uL6_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_uL6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_uL6-like_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_uL6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019907">
        <property type="entry name" value="Ribosomal_uL6_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002359">
        <property type="entry name" value="Ribosomal_uL6_CS2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004037">
        <property type="entry name" value="PRK05518.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03653">
        <property type="entry name" value="uL6_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF16">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="60S/50S RIBOSOMAL PROTEIN L6/L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="Ribosomal protein L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00700">
        <property type="entry name" value="RIBOSOMAL_L6_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="7">
        <location>
            <begin position="13"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="7">
        <location>
            <begin position="97"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="184" mass="20884" checksum="056338B1CC0BAFAF" modified="2017-09-27" version="1">MPIDAWVREEVEIPEGVEVTVENNVVKVKGPKGELEREFSYPGVKIFTEDGKVVVFKEFPRRRDIAIARTFRAHIANMIKGVTEGFTYKLKAVYSHFPMTIKVQGDEVLIENFLGEKNPRRAKILPGVTVKVRGQEVIVEGIDKEAVGQTAANIEQATRITKWDRRVFQDGVYIVEKAGKPIRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P721</accession>
    <name>A0A218P721_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6 7">RNA 3'-terminal phosphate cyclase</fullName>
            <shortName evidence="6">RNA cyclase</shortName>
            <shortName evidence="6">RNA-3'-phosphate cyclase</shortName>
            <ecNumber evidence="6 7">6.5.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rtcA</name>
        <name evidence="10" type="ORF">A3L08_03950</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + ATP = a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23976"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.5.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.4" evidence="6 7"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853798.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P721"/>
    <dbReference type="GeneID" id="33315395"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03950"/>
    <dbReference type="OrthoDB" id="7994at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003963">
        <property type="term" value="F:RNA-3'-phosphate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00874">
        <property type="entry name" value="RNA_Cyclase_Class_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.20:FF:000002">
        <property type="entry name" value="RNA terminal phosphate cyclase-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00200">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013791">
        <property type="entry name" value="RNA3'-term_phos_cycl_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023797">
        <property type="entry name" value="RNA3'_phos_cyclase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037136">
        <property type="entry name" value="RNA3'_phos_cyclase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000228">
        <property type="entry name" value="RNA3'_term_phos_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017770">
        <property type="entry name" value="RNA3'_term_phos_cyc_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020719">
        <property type="entry name" value="RNA3'_term_phos_cycl-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036553">
        <property type="entry name" value="RPTC_insert"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03399">
        <property type="entry name" value="RNA_3prim_cycl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096">
        <property type="entry name" value="RNA 3' TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096:SF0">
        <property type="entry name" value="RNA 3'-TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01137">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05189">
        <property type="entry name" value="RTC_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005378">
        <property type="entry name" value="RNA3'_term_phos_cycl_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52913">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase, RPTC, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01287">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase" evidence="8">
        <location>
            <begin position="10"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase insert" evidence="9">
        <location>
            <begin position="181"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="6">
        <location>
            <position position="308"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="284"/>
            <end position="288"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="NCBIfam" id="TIGR03399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="344" mass="36938" checksum="2F105C933E52833A" modified="2017-09-27" version="1">MGWVEIDGSYGEGGGQILRTAVALSVITGKAVRIYNIRANRPNSGLKPQHLHGILALKELSGARVKGAQVGSTELEFIPGEAKPKHVRVPISTAGSITLVLQALLPAMAFVGGSFEVTGGTDVPWSPPVDYLKHVTLFALGKMGLRVELEIRRRGHYPKGGGVVAGRVEPWEEKRPLVALEWEKVERFAGISHATNLPAHVAERQAKAARERIGELYNVPVEIETEVSRSLGPGSGIVVWAETDMLRLGGDALGKRGKPAEVVGREAADELLDQLKTRAAVDKFLGDQLIPFLAFAGGEIGVAEITNHLVTNVWVVEKFLGKVFEVEGEVGGPGVVRVVREVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P733</accession>
    <name>A0A218P733_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein uS13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rps13</name>
        <name evidence="8" type="ORF">A3L08_04215</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit. Forms a loose heterodimer with protein S19. Forms two bridges to the 50S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853845.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P733"/>
    <dbReference type="GeneID" id="33315447"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04215"/>
    <dbReference type="OrthoDB" id="372127at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.50:FF:000001">
        <property type="entry name" value="30S ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="entry name" value="30s ribosomal protein s13, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01315">
        <property type="entry name" value="Ribosomal_uS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="Rbsml_uS13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_uS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_uS13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019977">
        <property type="entry name" value="Ribosomal_uS13_archaeal"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003140">
        <property type="entry name" value="PRK04053.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03629">
        <property type="entry name" value="uS13_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S13/40S RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002134">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="S13-like H2TH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="149" mass="17006" checksum="349D7C5A215C0126" modified="2017-09-27" version="1">MTDNFRHIVRVAGVDLDGNKQLRWALTGIRGIGINFATMVLRVAGIDPFMKAGYLTDEQVKEIEKILEDPVAHGIPAWAVNRPKDYETGKDMHLVTAKLAMAWREDVNRLRRIKSYRGIRLERGLPVRGQRTRSNFRHGTTLGVSRKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P738</accession>
    <name>A0A218P738_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Signal recognition particle 54 kDa protein</fullName>
            <shortName evidence="10">SRP54</shortName>
            <ecNumber evidence="10">3.6.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">srp54</name>
        <name evidence="13" type="ORF">A3L08_04335</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.4"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9 10">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. Archaeal SRP consists of a 7S RNA molecule of 300 nucleotides and two protein subunits: SRP54 and SRP19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="10">The SRP-RNC complex is targeted to the cytoplasmic membrane.</text>
    </comment>
    <comment type="domain">
        <text evidence="10">Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.4" evidence="10"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853863.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P738"/>
    <dbReference type="GeneID" id="33315471"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04335"/>
    <dbReference type="OrthoDB" id="52849at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17875">
        <property type="entry name" value="SRP54_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000022">
        <property type="entry name" value="Signal recognition particle 54 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="entry name" value="Signal recognition particle, SRP54 subunit, M-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 54K PROTEIN SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564:SF5">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="Signal peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 10" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="12">
        <location>
            <begin position="268"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="299"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="107"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="189"/>
            <end position="193"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="247"/>
            <end position="250"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="448" mass="49994" checksum="BD6130CA22E6C8B6" modified="2017-09-27" version="1">MALEKLGKALNSALKKLARSRTVDEATIKEVVRDIQRALIQADVNVRMVLQLTKTIEKRALEEEPPAGASKKEHIIQIVYEELTKFLGKEAKPLEIKEKPTILLTVGIQGSGKTTSVAKLARHLQKRGYKVGLVCSDTWRPGAYFQLKQLVEPLGIEVFGDPEEKDAVKLAREGVEYFKEKGVDVIIVDSAGRHKEESGLIEEMRQISEAIKPHEVILVIDGTIGQQAYNQALAFKEATPIGSIIVTKLDGSAKGGGALSAVAATGAPIKFIGVGERIDDLEAFDPKRFVSRLLGMGDIEGLLEKLEELQKQQEFKEEDMEKFLKGKFNLKDMYAQLEAMQNMGPLKQILQMIPGLGYSLPDDMVKVGEEKLRKYRVIMDSMTDEELENPEIINYSRIKRIARGSGTSTAEVRELLNQYNQMKKMFKSMDKRKLAKMAKKFNFGGLGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P744</accession>
    <name>A0A218P744_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">FUN14 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_04295</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FUN14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853856.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P744"/>
    <dbReference type="GeneID" id="33315463"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04295"/>
    <dbReference type="OrthoDB" id="85289at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007014">
        <property type="entry name" value="FUN14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04930">
        <property type="entry name" value="FUN14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="99" mass="10601" checksum="ACD65A8D670537FD" modified="2017-09-27" version="1">MEFDVNAMMGDMGVGAVVGFLTGYAIKKVMKLALALIGAYVVSLLWLEQKGVLIIDKDRLFNLVGEWSHEVLTAGEKVMALLPGTAAFLGGFALGFHRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P757</accession>
    <name>A0A218P757_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">KaiC-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_04400</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_198362136.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P757"/>
    <dbReference type="GeneID" id="33315484"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04400"/>
    <dbReference type="OrthoDB" id="85349at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="226" mass="25380" checksum="AF297F4231B8C887" modified="2017-09-27" version="1">MDPLEIMAMSTRQLSPTLISHEIGSDVEVIIYHLIKMLQPDYEKVVVISFNDAYYSVVKYLRAVYPDVDATLKNVVLVSINPLVEESMNPDLILRTSDPEIIGGRIRAAFSGMSNVLYIVLGLDIYGVRRTRDLPVMIPAITKTLSLGGNNGILMAFNVKLFPENIVELVNSFSLSVFRLSVKVEGESLKRVLTVVRSPFLEYNLKSWSYMVTKGGLIFTPEKEPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P779</accession>
    <name>A0A218P779_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Putative nickel-responsive regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_04490</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Transcriptional regulator.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
        <text evidence="8">Binds 1 nickel ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the transcriptional regulatory CopG/NikR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853888.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P779"/>
    <dbReference type="GeneID" id="33315502"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04490"/>
    <dbReference type="OrthoDB" id="25654at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010045">
        <property type="term" value="P:response to nickel cation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22231">
        <property type="entry name" value="RHH_NikR_HicB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="entry name" value="ACT-like. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="entry name" value="Met repressor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00476">
        <property type="entry name" value="NikR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050192">
        <property type="entry name" value="CopG/NikR_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022988">
        <property type="entry name" value="Ni_resp_reg_NikR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014864">
        <property type="entry name" value="TF_NikR_Ni-bd_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001884">
        <property type="entry name" value="PRK00630.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002169">
        <property type="entry name" value="PRK01002.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002815">
        <property type="entry name" value="PRK02967.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003381">
        <property type="entry name" value="PRK04460.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34719">
        <property type="entry name" value="NICKEL-RESPONSIVE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34719:SF2">
        <property type="entry name" value="NICKEL-RESPONSIVE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08753">
        <property type="entry name" value="NikR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8" id="KW-0533">Nickel</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Ribbon-helix-helix protein CopG" evidence="9">
        <location>
            <begin position="5"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor NikR nickel binding C-terminal" evidence="10">
        <location>
            <begin position="55"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="138" mass="15885" checksum="E70F9C3A382B89F5" modified="2017-09-27" version="1">MKITRFGVSVPEELLEKFDRIIEEKGYVNRSEAIRDMMRDFIVRHEWEQGEGEVAGTITMLYNHDEAEVVKELLDLQHDYLEEIISSIHVHMDEHNCLEVVIVKGKASRIKEIADRLLSLKGVKHGKLVMTGTGKELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P795</accession>
    <name>A0A218P795_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Arginase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_04630</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the arginase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853913.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P795"/>
    <dbReference type="GeneID" id="33315530"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04630"/>
    <dbReference type="OrthoDB" id="85348at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033389">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via agmatine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="ARGINASE/AGMATINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358:SF26">
        <property type="entry name" value="GUANIDINO ACID HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="241" mass="28211" checksum="F3CE247298BA6E80" modified="2017-09-27" version="1">MVTFIPFGEKPNRDGVLYVLQFLKRNKLIEDYMIVESSRVELLPDRIPQDRAYIIGEHLATYGIVEKLRPNSLLSIDAHTDLMHDYLDHGSWLAYALKERLVNRAVVLAPVLMIPTTERTQLWTRRVKIFPALLRSRKVRGKWRAYKNLQTNDLLEILDETKKYLGREIYLTVDLDVLRPEYKIARFQHGELTLEELLEILEEVKKNFKISAFDIAEVSDRIRRSKLGKKAFAEVFQLLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7A9</accession>
    <name>A0A218P7A9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Proteasome subunit beta</fullName>
            <ecNumber evidence="9">3.4.25.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">20S proteasome beta subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Proteasome core protein PsmB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">psmB</name>
        <name evidence="11" type="ORF">A3L08_04535</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.25.1" evidence="9"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853896.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7A9"/>
    <dbReference type="GeneID" id="33315511"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04535"/>
    <dbReference type="OrthoDB" id="6330at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000049">
        <property type="entry name" value="Proteasome subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02113_A">
        <property type="entry name" value="Proteasome_B_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019983">
        <property type="entry name" value="Pept_T1A_Psome_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03634">
        <property type="entry name" value="arc_protsome_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF0">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE SUBUNIT HSLV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0645">Protease</keyword>
    <keyword evidence="7 9 11" id="KW-0647">Proteasome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0888">Threonine protease</keyword>
    <keyword evidence="8 9" id="KW-0865">Zymogen</keyword>
    <feature type="propeptide" id="PRO_5011123583" description="Removed in mature form; by autocatalysis" evidence="9">
        <location>
            <begin position="1"/>
            <end position="7"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023371380" description="Proteasome subunit beta" evidence="9">
        <location>
            <begin position="8"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9 10">
        <location>
            <position position="8"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="203" mass="21910" checksum="6F07CD3A2505EE14" modified="2017-09-27" version="1">MAEKLKGTTTVGIVCRDGVVLAADRRASLGNMVLSENVTKVFWIDDHLALAGAGSVGDILSIVRLLRAEAKLYRAKVGREMSVKALATLTSNILHGGRGYAYLAWFLVGGYDSAPRLYSIDAAGGVTEDRFTAAGSGMEFAFSILETEFEEDMPLKEGVKLALRAIKAATRRDVFTGGGVTLVTITEEGYKEWTEEEIKALLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7E4</accession>
    <name>A0A218P7E4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UPF0292 protein A3L08_04860</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_04860</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPF0292 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853951.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7E4"/>
    <dbReference type="GeneID" id="33315576"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04860"/>
    <dbReference type="OrthoDB" id="56459at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01027">
        <property type="entry name" value="TOPRIM_RNase_M5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01095">
        <property type="entry name" value="UPF0292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034141">
        <property type="entry name" value="TOPRIM_RNase_M5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022972">
        <property type="entry name" value="UPF0292"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003090">
        <property type="entry name" value="PRK04017.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39964:SF2">
        <property type="entry name" value="UPF0292 PROTEIN MJ1624"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39964">
        <property type="entry name" value="UPF0292 PROTEIN TK1411"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110455">
        <property type="entry name" value="Toprim domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Toprim" evidence="4">
        <location>
            <begin position="20"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="133" mass="15448" checksum="6544B37F35722DD6" modified="2017-09-27" version="1">MYAENYKRFLELIDKLREFEGALIVEGMRDEVALRNLGVRAEIIRLSRLPLTEVALIASSYKEVMILTDFDRKGEELARKLLQYLEGYPCRVDAETRRELKRIARKDIKGVEDLYGLYLKVISVSDPLPEGIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7F3</accession>
    <name>A0A218P7F3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sucrose transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_04925</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853961.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7F3"/>
    <dbReference type="GeneID" id="33315589"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04925"/>
    <dbReference type="OrthoDB" id="85689at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17313">
        <property type="entry name" value="MFS_SLC45_SUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23528">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23528:SF1">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="313"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="351"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="381"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="415"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="7"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="453" mass="50355" checksum="C71B522FD9F126F6" modified="2017-09-27" version="1">MEFKYSRIFLLGFGFFGISIIWSLYNAYIPIFLQDTFGMSRTATGFVMTIDNLFAVLLLPFLGALSDMTRTRLGRRKPYILLGAPSAAIMFALIPIAREHGSLALFMGTIIFMNFFMALFRSPVVAFMPDITPSEKRSQANGIINFMGGLGALLAYFGGKVLYDMNYAYPFYAGAAIMLLANLLVVFFVPEPEEYRVPGEKVSIRKLLSETSRKSFGELKENLKDVFASHERSLLFILVSIFFWFVAFNSLETFFTSYFKYHLFRIPVGAAETEFTRKIESTGAFLLGAVSLSFMISAIPAGFLGARIGRKRTITAGLILISLVVLGAYFLGETHQPQSPSLSDPVVMEFMALFFVAGIGWAMVNVNSLPMVVDMTTEEKLGGYTGLYYFFSQAANLVAPPLAGAFLDLIGYETLLPFAVVFFILAAITMQFVRRGDIVRTKGNALDYVPDMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7G0</accession>
    <name>A0A218P7G0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_04970</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853969.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7G0"/>
    <dbReference type="GeneID" id="33315598"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04970"/>
    <dbReference type="OrthoDB" id="86157at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="67" mass="8288" checksum="04EDE10BEC575F11" modified="2017-09-27" version="1">MGKVDDALFEARPYVEYWDRLKELVEKLWDEVESEEEFIKRIMEEMEKAEEPFKTDLRIFLQKFEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7H7</accession>
    <name>A0A218P7H7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rps12</name>
        <name evidence="10" type="ORF">A3L08_05060</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">With S4 and S5 plays an important role in translational accuracy. Located at the interface of the 30S and 50S subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7 9">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_014122317.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7H7"/>
    <dbReference type="SMR" id="A0A218P7H7"/>
    <dbReference type="GeneID" id="33325075"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05060"/>
    <dbReference type="OrthoDB" id="45154at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03367">
        <property type="entry name" value="Ribosomal_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000007">
        <property type="entry name" value="40S ribosomal protein S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00403_A">
        <property type="entry name" value="Ribosomal_uS12_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_uS12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022863">
        <property type="entry name" value="Ribosomal_uS12_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005679">
        <property type="entry name" value="Ribosomal_uS12_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005680">
        <property type="entry name" value="Ribosomal_uS12_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003254">
        <property type="entry name" value="PRK04211.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00982">
        <property type="entry name" value="uS12_E_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002133">
        <property type="entry name" value="Ribosomal_S12/S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01034">
        <property type="entry name" value="RIBOSOMALS12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7 9" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="147" mass="16421" checksum="C55DB32035C5818A" modified="2017-09-27" version="1">MAGKKAPYGEFAGRKLKLKRKKFRWSDIRYKRRVLRLKEKSDPLEGAPQAKGIVLEKIAVEAKQPNSAMRKAVRVQLIKNGKVVTAFTPGDGAINHIDEHDEVIIEGIGGPKGGSMGDIPGIRYKVVKVNRVSLKELVKGRKEKPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7I8</accession>
    <name>A0A218P7I8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein eL42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl44e</name>
        <name evidence="8" type="ORF">A3L08_05130</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the eukaryotic ribosomal protein eL42 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012571193.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7I8"/>
    <dbReference type="GeneID" id="28495965"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05130"/>
    <dbReference type="OrthoDB" id="52456at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.80:FF:000001">
        <property type="entry name" value="60S ribosomal protein L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01476">
        <property type="entry name" value="Ribosomal_L44e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000552">
        <property type="entry name" value="Ribosomal_eL44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053708">
        <property type="entry name" value="Ribosomal_LSU_eL42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004425">
        <property type="entry name" value="PRK05767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10369">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36A/L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00935">
        <property type="entry name" value="Ribosomal_L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01172">
        <property type="entry name" value="RIBOSOMAL_L44E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="3 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="zinc finger region" description="C4-type" evidence="6">
        <location>
            <begin position="11"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="94" mass="11259" checksum="593DB1D145DE2DF5" modified="2017-09-27" version="1">MKYPKQIRTYCPFCKKHTIHKVEKVKKRPRSELSQGQRRFRRIMKGYRGFPRPNPAGREKPVKKLDLRFRCTVCGKAHTRGQGFRVKKFELVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7N2</accession>
    <name>A0A218P7N2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0212 protein A3L08_05445</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0212 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854052.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7N2"/>
    <dbReference type="GeneID" id="33315693"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05445"/>
    <dbReference type="OrthoDB" id="63517at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01223">
        <property type="entry name" value="UPF0212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007564">
        <property type="entry name" value="UPF0212"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003035">
        <property type="entry name" value="PRK03922.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42199">
        <property type="entry name" value="UPF0212 PROTEIN MJ0068"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42199:SF1">
        <property type="entry name" value="UPF0212 PROTEIN TK1194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04475">
        <property type="entry name" value="DUF555"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016934">
        <property type="entry name" value="UCP016934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="126" mass="13725" checksum="C0D6B12211202ECC" modified="2017-09-27" version="1">MGDYVVVLEAPIIVRDVETSEDAINVAVSKVAKALNKEKLDFVRVEIGYSQCPVCGAHFESAFVIGSVGLVGMYLTIKVYNAQTVEHAERIAKAVIGKALKKVPLKVYEIRELTEEDEGDGVELGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7R3</accession>
    <name>A0A218P7R3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Adenylate cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854068.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7R3"/>
    <dbReference type="GeneID" id="33315710"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05530"/>
    <dbReference type="OrthoDB" id="46040at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07890">
        <property type="entry name" value="CYTH-like_AC_IV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.320.10">
        <property type="entry name" value="Hypothetical Protein Pfu-838710-001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008173">
        <property type="entry name" value="Adenylyl_cyclase_CyaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033469">
        <property type="entry name" value="CYTH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023577">
        <property type="entry name" value="CYTH_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00318">
        <property type="entry name" value="cyaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21028:SF2">
        <property type="entry name" value="CYTH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21028">
        <property type="entry name" value="SI:CH211-156B7.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01928">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01118">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55154">
        <property type="entry name" value="CYTH-like phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51707">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CYTH" evidence="1">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="177" mass="21180" checksum="590219DFDCDF4AD7" modified="2017-09-27" version="1">MIEIEVKGYADDKIFERVREDFELIRREYHEDTYFQHPCRDFTETDEALRIRVRRFNGHFEAFLTYKGPKIDPNSKTRREIEVPISDPDKHSEILSRLGFQEVLTVEKVREKYYVDKGIIIALDEVEGLGKFIEIEALAENERAVEETVERLRGILKELGVERFERRSYLELLLERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7S4</accession>
    <name>A0A218P7S4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">N-glycosylase/DNA lyase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">8-oxoguanine DNA glycosylase</fullName>
            <ecNumber evidence="6">3.2.2.-</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">AGOG</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">DNA-(apurinic or apyrimidinic site) lyase</fullName>
            <shortName evidence="6">AP lyase</shortName>
            <ecNumber evidence="6">4.2.99.18</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_05640</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="6">Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif).</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the archaeal N-glycosylase/DNA lyase (AGOG) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="6"/>
    <dbReference type="EC" id="4.2.99.18" evidence="6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854088.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7S4"/>
    <dbReference type="GeneID" id="33315732"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05640"/>
    <dbReference type="OrthoDB" id="15106at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000702">
        <property type="term" value="F:oxidized base lesion DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01168">
        <property type="entry name" value="AGOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016544">
        <property type="entry name" value="AGOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015254">
        <property type="entry name" value="AGOG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009785">
        <property type="entry name" value="PRK13280.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09171">
        <property type="entry name" value="AGOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008955">
        <property type="entry name" value="AGOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="2 6" id="KW-0228">DNA excision</keyword>
    <keyword evidence="4 6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Schiff-base intermediate with DNA" evidence="6">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>8-oxoguanine</name>
            <dbReference type="ChEBI" id="CHEBI:52617"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>8-oxoguanine</name>
            <dbReference type="ChEBI" id="CHEBI:52617"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>8-oxoguanine</name>
            <dbReference type="ChEBI" id="CHEBI:52617"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>8-oxoguanine</name>
            <dbReference type="ChEBI" id="CHEBI:52617"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>8-oxoguanine</name>
            <dbReference type="ChEBI" id="CHEBI:52617"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>8-oxoguanine</name>
            <dbReference type="ChEBI" id="CHEBI:52617"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>8-oxoguanine</name>
            <dbReference type="ChEBI" id="CHEBI:52617"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="263" mass="30077" checksum="3294BDB4A79C0FED" modified="2017-09-27" version="1">MTLDRFIQIKYKSDDEKVRELVSILRELGLECARTIEEHVDLQFDALKNLRGNLGDDETFIKLVVANSIVSYQLSAKGEQWWWEFSHYFSANPPERGIAEAYADFLPNSRTNRRLVSGKVKRLEKLEPFLERLDMDALKGYYFGGMERLRDDLARALGSKRSAKTVVFAVKMFGYAGRIAFGEFVPYPMGIEIPDDVRINAYTKRFTNEPPVSFWKKVAEKTGIPPLHIDSILWPVLGGNPEVLRRLEEHCEESDAVLGLASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7W4</accession>
    <name>A0A218P7W4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">HIT family hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_05880</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854135.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7W4"/>
    <dbReference type="GeneID" id="33315780"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05880"/>
    <dbReference type="OrthoDB" id="26806at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01275">
        <property type="entry name" value="FHIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052908">
        <property type="entry name" value="AP-4-A_phosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039383">
        <property type="entry name" value="FHIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42997:SF1">
        <property type="entry name" value="AP-4-A PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42997">
        <property type="entry name" value="HIT FAMILY HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="3">
        <location>
            <begin position="22"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="2">
        <location>
            <begin position="117"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="164" mass="19025" checksum="31E287F69808AF66" modified="2017-09-27" version="1">MKVLWAPWRIEYIRSPKHKGCIFCDFPKENRDKERLILYRGKHSFVIMNNYPYNPGHVMIAPYRHVGKWEDLTDEELLEIMKLSQLMIKALKRAMNPDGFNLGVNLGRVAGAGIDDHVHLHIVPRWNGDTNFMPVIADTKVIPESLEEAYNELKKAIEEVEGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P806</accession>
    <name>A0A218P806_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06075</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854169.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P806"/>
    <dbReference type="GeneID" id="33315818"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06075"/>
    <dbReference type="OrthoDB" id="98800at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="201" mass="21745" checksum="9CBABEC3F4010512" modified="2017-09-27" version="1">MGKTPPWKALFTFPAALAILGLLWGTGPSVTYVQVNGTFSGGMVVPSTIADEVEDYFSNVNATLYSFEAKVVGEMNASITTYALKVTPPFDPDGFEIIINAHPVNGTTYVPYAEGIPVSVRYMGHSYRSVLTVRPTRSVGSFGEWSEEYLGGANGSRLLKVDSLTLVVDVVEPGHYDFVIVKEPENLEISRDGLILEGNTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P831</accession>
    <name>A0A218P831_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Stage II sporulation protein M</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06275</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854205.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P831"/>
    <dbReference type="GeneID" id="33315858"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06275"/>
    <dbReference type="OrthoDB" id="86288at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002798">
        <property type="entry name" value="SpoIIM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35337">
        <property type="entry name" value="SLR1478 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35337:SF1">
        <property type="entry name" value="SLR1478 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01944">
        <property type="entry name" value="SpoIIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="20203" checksum="C1F64F59D26086D7" modified="2017-09-27" version="1">MRLPEIPRRTFVLLLMVFMVFSFVGYAAGAANPEAAVEAVKKVISQIGPISDSSFQNFIKIFTNNSLVALFMFISGLFFGLGPWFIMAFNGLVVGLVVLAVHRTAGMPMSQVILALVPHGVIEIPAIAIAGVAGIVWYRELVKGEGEPAERFKRGMMEGFKLYLLSVALLLVAALVEAYVTPKVAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P844</accession>
    <name>A0A218P844_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KEOPS complex subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06390</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854225.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P844"/>
    <dbReference type="GeneID" id="33315881"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06390"/>
    <dbReference type="OrthoDB" id="85338at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011470">
        <property type="entry name" value="PRK14887.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="85" mass="9162" checksum="9268199A04CDCDF8" modified="2017-09-27" version="1">MRIEARAEIVWHYGDPVKAEAIAGAVQVDNEGIPPGLKKSLNVVSLSEDGDVITKVKYSGEIETLIKALDDLVFAVKIAEEALNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P845</accession>
    <name>A0A218P845_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UDP-N-acetyl glucosamine 2-epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854202.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P845"/>
    <dbReference type="GeneID" id="33315855"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06260"/>
    <dbReference type="OrthoDB" id="7018at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd03786">
        <property type="entry name" value="GTB_UDP-GlcNAc_2-Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003331">
        <property type="entry name" value="UDP_GlcNAc_Epimerase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029767">
        <property type="entry name" value="WecB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00236">
        <property type="entry name" value="wecB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43174">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE 2-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43174:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE 2-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02350">
        <property type="entry name" value="Epimerase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDP-N-acetylglucosamine 2-epimerase" evidence="1">
        <location>
            <begin position="27"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="377" mass="42361" checksum="B61E0814B27E967F" modified="2017-09-27" version="1">MRPAFVFGTRPEIIKLSPVIRAFIKRGVEPLLIHTGQHYDYEMSAVFLEELELPEIDYHLEVGSGTQAEQTGLAMMKIEKVLMDEKPDVTLVQGDTNTVLAGALASVKLKIPVAHVEAGLRSFDRTMPEEINRVLADHASEVLFPPTEEARRNLEREGITENVYVTGNTIVDAVLQNAEMAEKKSDVLERFSLKPGEYILITAHRAENTDSRENLEKLIEILESLPMRAIYPMHPRTKNRLKEFGLLGKVEGIENLIVTKPLGYLDFLKLERNAFAIMTDSGGIQEEAIILNVPCLTLRYNTERPETVEAGGNVLVGLEKERALNYINKLLNDAEFYRKMASAPNPFGDGKAGERIAEILLKLHERGELKVRSSRFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P870</accession>
    <name>A0A218P870_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06345</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854218.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P870"/>
    <dbReference type="GeneID" id="33315872"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06345"/>
    <dbReference type="OrthoDB" id="85398at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="188" mass="21009" checksum="B9F89D1A8291C2CF" modified="2017-09-27" version="1">MGLVHVSCESASVEDCVNDFRKKLKESLPVSGCYIKSAELNLTFGAFMHLSAILLVDTSRSGGGVIVEYSTGRNREDAIQRVLEKINPVIEDAEVVSFKVETYTTPVTRRTYAVGVAVYNLSPRSHGPLTETADRRKILAHVLSLFDYNPKVLNISELARVFGVSRDTIYYDIQRILEEKKKEGDHLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P894</accession>
    <name>A0A218P894_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Hydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_06530</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P894"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06530"/>
    <dbReference type="OrthoDB" id="23478at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010226">
        <property type="entry name" value="NADH_quinone_OxRdtase_chainI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849:SF35">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 8, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="20"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="55"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="163" mass="18192" checksum="FCDF2B252FFF7F9A" modified="2017-09-27" version="1">MPVTKMYPFEPAEAPPEYRGIPRIDPVLCIGCGACANACPPNAILRIDDPESGMRRIVLDVGRCIRCARCEEVCPTGAARLTAEFEAATDDRRDHVEVVELKLARCRNCGRYTHYTERQVAKVLSLIPRGILDFDRVREKLPLCSECKLKLTVLNATKFGEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8A3</accession>
    <name>A0A218P8A3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_06590</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854263.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8A3"/>
    <dbReference type="GeneID" id="33315921"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06590"/>
    <dbReference type="OrthoDB" id="2837at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16374">
        <property type="entry name" value="DMSOR_beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000813">
        <property type="entry name" value="7Fe_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050954">
        <property type="entry name" value="ET_IronSulfur_Cluster-Binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43177">
        <property type="entry name" value="PROTEIN NRFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43177:SF3">
        <property type="entry name" value="PROTEIN NRFC HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13247">
        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00354">
        <property type="entry name" value="7FE8SFRDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5 8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 8" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="9">
        <location>
            <begin position="4"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="9">
        <location>
            <begin position="71"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ07013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="168" mass="18638" checksum="97798B5ED3C2E449" modified="2017-09-27" version="1">MTKKILHVDYSLCIGCETCQGVCDFIHDGKPNIRIYYTVSGLPVPINCRHCERAPCLEICPVGAIYKDHEGAVIIDPGKCIGCLMCLAVCPFGVPSFDVRIRVVTKCDMCAERRAEGMEPACGEMCPAEAIFFGESEEIEDRIMRRTAEKIARERISSMNLEGVGRML</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8A6</accession>
    <name>A0A218P8A6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cation:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_06570</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854259.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8A6"/>
    <dbReference type="GeneID" id="33315917"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06570"/>
    <dbReference type="OrthoDB" id="85180at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002758">
        <property type="entry name" value="Cation_antiport_E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34584">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER SUBUNIT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34584:SF1">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER SUBUNIT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01899">
        <property type="entry name" value="MNHE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019239">
        <property type="entry name" value="MrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="164" mass="18294" checksum="20BDAED7930C7F69" modified="2017-09-27" version="1">MRSVPVMILAFIIYIIVTGSITAYDVITGAVTALAAGLLFGRHLVSNPRKALNPVRWGRFALYFLKYITVIEAKAHADVIRRIISGDVRPGIVKVPLDLRDEYARFLVAASITNTPGTVTVHMDDRYAYVNWINVSTSDPEKRRKEILEEFEENAKKIFEGGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8B6</accession>
    <name>A0A218P8B6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PRC-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854272.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8B6"/>
    <dbReference type="GeneID" id="33315931"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06640"/>
    <dbReference type="OrthoDB" id="68960at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.240">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38137">
        <property type="entry name" value="PRC-BARREL DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38137:SF1">
        <property type="entry name" value="PRC-BARREL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC-barrel" evidence="1">
        <location>
            <begin position="2"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="69" mass="7759" checksum="90590FAD4BF5ABDC" modified="2017-09-27" version="1">MVMRLSKLYGKQIYNTRGYYVGYVDEILIEIDRGRGKVLALGLPGEKVGVPYERVTAIGDIILVKAKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8D8</accession>
    <name>A0A218P8D8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Formate hydrogenlyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_06515</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854249.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8D8"/>
    <dbReference type="GeneID" id="33315906"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06515"/>
    <dbReference type="OrthoDB" id="371891at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052175">
        <property type="entry name" value="ComplexI-like_HydComp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003918">
        <property type="entry name" value="NADH_UbQ_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42682:SF3">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42682">
        <property type="entry name" value="HYDROGENASE-4 COMPONENT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01437">
        <property type="entry name" value="NUOXDRDTASE4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="215"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="344"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="426"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="459"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="571"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="8">
        <location>
            <begin position="106"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="593" mass="63509" checksum="EC0FEAA934B77A5A" modified="2017-09-27" version="1">MIFALVFLLAIITGLVLRNSKVVSALSALASLSLVIEAARGITFHASISGITFAFATDELSRFFAILVGVAGSAVSVYSLSYMGRRHLVTVAYPLFVLSMALIVLARDFLTLLIFWELMTAASYVLIVYNYEKEETRRASVVYLVTMHALNTAPLIMGLGYVCSHAGTLDYSALSGTVVPSWVIWALTIGFLAKAGIFPMHFWLPEAHPVAPSNVSALLSGVMLKMAVYGMLRTLETFGGVDSIAVPIVVLSIITIALGSLLALNQKDIKRMMAYSSIDNMGYIFLAIGAYLLLPGELKEVALTAVLLHSFNHMLFKNLLFMLSGNILHATGRRDTGIRGLAKEMPITFGLSIVGILAVSGVPPLNGFASKWLIYRATFLSKDPLLVVGGAVALLGSALTLAAYLKLYRVFTGEPNVEAEEAPLPMLLGEGILAALCVVGGVVPGLFLHPVGLSYPAELNLPLLALVIAVLIASLLVALPPRARKGGIWTNGEPVEEFEIKPEHMYTGVSEAVQSISAAGGRIQLLALSMGRLYRSTEVGYIDEALFMPLIRLSYGFGAYLRELSKGLNGMLATAFLVLVAMVIAFLAFAGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8E2</accession>
    <name>A0A218P8E2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribonuclease VapC</fullName>
            <shortName evidence="5">RNase VapC</shortName>
            <ecNumber evidence="5">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Putative toxin VapC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">vapC</name>
        <name evidence="7" type="ORF">A3L08_06800</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Toxic component of a toxin-antitoxin (TA) system. An RNase.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PINc/VapC protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="5"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8E2"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06800"/>
    <dbReference type="OrthoDB" id="41298at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09854">
        <property type="entry name" value="PIN_VapC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00265">
        <property type="entry name" value="VapC_Nob1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039018">
        <property type="entry name" value="VapC20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022907">
        <property type="entry name" value="VapC_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42188">
        <property type="entry name" value="23S RRNA-SPECIFIC ENDONUCLEASE VAPC20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42188:SF1">
        <property type="entry name" value="23S RRNA-SPECIFIC ENDONUCLEASE VAPC20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0800">Toxin</keyword>
    <keyword evidence="1 5" id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="domain" description="PIN" evidence="6">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="5"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="138" mass="15564" checksum="D3038B7B5B6B5DA3" modified="2017-09-27" version="1">MRFIDANVFIYAFLKPRREPPDTVKAIKEKAKGILRRVSDGEEVVTTVIHLSEVANVVESRAGKGKAAEIVLSVLTSQNIRVLDVSTADYLRAALIAEERNLGINDALAYLKMRELGIDEIYTFDRDFEKLDVKIVRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8E9</accession>
    <name>A0A218P8E9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_06845</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854308.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8E9"/>
    <dbReference type="GeneID" id="33315972"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06845"/>
    <dbReference type="OrthoDB" id="50488at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050492">
        <property type="entry name" value="Bact_metal-bind_prot9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006128">
        <property type="entry name" value="Lipoprotein_PsaA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006127">
        <property type="entry name" value="ZnuA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42953">
        <property type="entry name" value="HIGH-AFFINITY ZINC UPTAKE SYSTEM PROTEIN ZNUA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42953:SF1">
        <property type="entry name" value="METAL-BINDING PROTEIN HI_0362-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01297">
        <property type="entry name" value="ZnuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00690">
        <property type="entry name" value="ADHESNFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53807">
        <property type="entry name" value="Helical backbone' metal receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="307"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="328" mass="35279" checksum="F6499CA49A12A68D" modified="2017-09-27" version="1">MKVKAVLFVLLIAGVLFTPGVSGSEGKPLVVTTIGPLAGIISDAFGNSVDVVYLIPPGADPHEYQLTPSQIELLRKADVIVTTGGHLPVEKRIAELREEGTITGEALFIEDYKANGFRYLKEHWYSGKDNPHGVWLDPTNALAIARAAERALEKADPGNAEVYAEEYERFEGRVKAIADSYRALVGQNHTAVIQMPPDQYAIEWLGIKAVASIKPEEEVPAIGVDDLVPTAKTADLIVYGIDSPDQMKRAALELASKSGKPAAEITVFWSDRPYTEVLIENSAAVVKALSGNAPVEKPEGRSDVTQYVLISLVVGIVLGTALGVVLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8F6</accession>
    <name>A0A218P8F6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="3 6">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_06870</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FKBP-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="3 6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8F6"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06870"/>
    <dbReference type="OrthoDB" id="8615at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054016">
        <property type="entry name" value="FKBP26_IF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048261">
        <property type="entry name" value="SlpA/SlyD-like_ins_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47861">
        <property type="entry name" value="FKBP-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE SLYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47861:SF2">
        <property type="entry name" value="LONG-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22199">
        <property type="entry name" value="FKBP26_IF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="8">
        <location>
            <begin position="7"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="233"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="239"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="263"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="277"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="318"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="346"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="357" mass="40407" checksum="925263EA4F0DE227" modified="2017-09-27" version="1">MTKVQRGDVIRLHYTGKVKETGEVFDTTYEEVAKEAGIYNEKGIYGPVPIAVGAGHVLKGLDEQLEGLEVGEKYEIIVPPEKGFGKRDPKLIKTFTLGQFRRQGIYPFPGMPVEIETEGGRKLRGRVLTVSGGRVRVDFNHPYAGKHLVYEVEVVEKIDDPIEKVKALMELRMPNVDREKVIIEVGENDVTVDFTPVLGEVDKNTLILGEIILESDLKFLGYEEITFKPSVDELLKPPEEEEESKEVVELEEEVSEPLVEKAEETEEKEATAEDTEEKPAEEKPAEEETKEEEKAEAAEAKEEEKEVTEEKTEEKTKKTTGKKKTTKGRKTRRTTTRKTTSKKKTKASEKKEEPKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8F9</accession>
    <name>A0A218P8F9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_06840</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854307.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8F9"/>
    <dbReference type="GeneID" id="33315971"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06840"/>
    <dbReference type="OrthoDB" id="372203at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06580">
        <property type="entry name" value="TM_PBP1_transp_TpRbsC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43370:SF1">
        <property type="entry name" value="GUANOSINE ABC TRANSPORTER PERMEASE PROTEIN NUPQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43370">
        <property type="entry name" value="SUGAR ABC TRANSPORTER INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="297" mass="31708" checksum="7CEACB2357303FE5" modified="2017-09-27" version="1">MNAGDVITILITSLMAMVPIVLTSVGAAWSERAGVVSIGYEGVLLMSAFFGAIFAELSGHATIGLLGGVLTGVLLGMLHGALTVYLKGDHVIPGIGINLLAMGVVPFGILAYWGTAGQHQLPDNAELWHIKTSTVELSPMVFVTILIALVTWWVLFKTPLGLRVRAVGENPEAADALGINVEKYRFWSAVYGHALAGLAGAYMSVDWLGLVHKTMSGGRGFIALANMVFSGWNPLVSLIGGWLFGFFEALATWLAPKHIIPGQFILMLPYIMTLIIVAGIIGKARPPKADGRPYKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8H3</accession>
    <name>A0A218P8H3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HNH nuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_07050</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8H3"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07050"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00085">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002711">
        <property type="entry name" value="HNH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003615">
        <property type="entry name" value="HNH_nuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052892">
        <property type="entry name" value="NA-targeting_endonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33877:SF2">
        <property type="entry name" value="OS07G0170200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33877">
        <property type="entry name" value="SLL1193 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01844">
        <property type="entry name" value="HNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00507">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HNH nuclease" evidence="2">
        <location>
            <begin position="19"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="154" mass="18311" checksum="A04DEB382F6CDBB1" modified="2017-09-27" version="1">MFFWDDEKKSASRRDITGAMRRDIIEIVKRKCEYCGRRITTTTGYIHHITPVREGGKTTPSNIIVLCGDCHRKADNAIIKRTELRKKVRNRPKEVQSAIQRVLNRSKSRRPSNTKPRDEFSFIEPDFSFFDEHKKKRGRKRKKSDDDSWWPQFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8K5</accession>
    <name>A0A218P8K5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">tRNA-guanine(15) transglycosylase</fullName>
            <ecNumber evidence="7">2.4.2.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">7-cyano-7-deazaguanine tRNA-ribosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Archaeal tRNA-guanine transglycosylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tgtA</name>
        <name evidence="9" type="ORF">A3L08_07040</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Exchanges the guanine residue with 7-cyano-7-deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D-loop) of archaeal tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>guanosine(15) in tRNA + 7-cyano-7-carbaguanine = 7-cyano-7-carbaguanosine(15) in tRNA + guanine</text>
            <dbReference type="Rhea" id="RHEA:43164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10371"/>
            <dbReference type="Rhea" id="RHEA-COMP:10372"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:82850"/>
            <dbReference type="EC" id="2.4.2.48"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">tRNA modification; archaeosine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the archaeosine tRNA-ribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.48" evidence="7"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854343.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8K5"/>
    <dbReference type="GeneID" id="33316011"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07040"/>
    <dbReference type="OrthoDB" id="6871at2157"/>
    <dbReference type="UniPathway" id="UPA00393"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002099">
        <property type="term" value="P:tRNA wobble guanine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21149">
        <property type="entry name" value="PUA_archaeosine_TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1020.10">
        <property type="entry name" value="ArcTGT, C1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.90">
        <property type="entry name" value="ArcTGT, C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="entry name" value="Queuine tRNA-ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01634">
        <property type="entry name" value="TgtA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050076">
        <property type="entry name" value="ArchSynthase1/Queuine_TRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038370">
        <property type="entry name" value="ArcTGT_C1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032729">
        <property type="entry name" value="TGT_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029402">
        <property type="entry name" value="TGT_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038250">
        <property type="entry name" value="TGT_C2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004804">
        <property type="entry name" value="TgtA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00432">
        <property type="entry name" value="arcsn_tRNA_tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46499">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46499:SF1">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14809">
        <property type="entry name" value="TGT_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14810">
        <property type="entry name" value="TGT_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88802">
        <property type="entry name" value="Pre-PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="tRNA-guanine transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="6 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PUA" evidence="8">
        <location>
            <begin position="505"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="580" mass="65939" checksum="F5F16B9CF09F3985" modified="2017-09-27" version="1">MVDFRFEVKARDAAGRIGKLTVNGKTIETPAIMPVVNPKQLIVTPKELKEMGFGIIITNSYIIYKTPELREKALEVGIHRLLDYDGIIEVDSGSFQLMRYGGVEVTNREIVEFQHDIGVDIGTFLDIPTPPDAPREKAEEDLRITLERAKEAEEIKEIAMNAAVQGSTHLDLRTYAAKKLGEMNFEIHPVGAVVPLMESYRYKDLVDVVIASKQGLRPDRPVHLFGAGHPMIFALAVAMGIDLFDSASYALYAKDDRYLTPEGTKRLEELDYFPCSCPVCSRYTPQELREMPKEERTRLLALHNLWVIREELNRVKQAVKEGTLWELVDERARSHPKMFAAYKRLLEYREYLEKNEPVTKASAFFKVSEESLRWPTAVRAKERAERVKDKFPKTIEHPIFGEIPKYLSLSYPFAQSEGEKDFSIRKPSKDEARSYVMAIAEYQFGEGAGEAFRDAFVELSRKTGMPRQIKAKGKHLATFRAEDGLLTLGIEGARRLHEVLPFPRMRVVVNEDAEPFARKGKNVFAKFVIDADPEIRPYDEVLVVNERDELLATGQTLLSGEELKVFQSGLAVKVRRGIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8P9</accession>
    <name>A0A218P8P9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Translation initiation factor 1A</fullName>
            <shortName evidence="5">aIF-1A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">eif1a</name>
        <name evidence="11" type="ORF">A3L08_07320</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5 8">Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 7">Belongs to the eIF-1A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854396.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8P9"/>
    <dbReference type="GeneID" id="33316067"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07320"/>
    <dbReference type="OrthoDB" id="2586at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05793">
        <property type="entry name" value="S1_IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00216">
        <property type="entry name" value="aIF_1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001253">
        <property type="entry name" value="TIF_eIF-1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018104">
        <property type="entry name" value="TIF_eIF-1A_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00523">
        <property type="entry name" value="eIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003084">
        <property type="entry name" value="PRK04012.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003085">
        <property type="entry name" value="PRK04012.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003086">
        <property type="entry name" value="PRK04012.2-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21668">
        <property type="entry name" value="EIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00652">
        <property type="entry name" value="eIF1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01262">
        <property type="entry name" value="IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 5 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1-like" evidence="10">
        <location>
            <begin position="21"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ07144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="121" mass="14119" checksum="6FA02A5202F98371" modified="2017-09-27" version="1">MAYHRSGNNKKKKDRQVQGDEVIRVPLPKEGQLFGVIEQALGSGWMDVRCSDGKIRRCRIPGKLKRRMWMRVGDVVIVQPWEVQSDERGDIVYRYTRTQVDWLLRRGKISQDFISGGELLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8S3</accession>
    <name>A0A218P8S3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable cyclic pyranopterin monophosphate synthase</fullName>
            <ecNumber evidence="3">4.6.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Molybdenum cofactor biosynthesis protein C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">moaC</name>
        <name evidence="5" type="ORF">A3L08_07570</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the conversion of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate to cyclic pyranopterin monophosphate (cPMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate = cyclic pyranopterin phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:49580"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59648"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.6.1.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 3">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homohexamer; trimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MoaC family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.17" evidence="3"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854438.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8S3"/>
    <dbReference type="GeneID" id="33316117"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07570"/>
    <dbReference type="OrthoDB" id="10067at2157"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061798">
        <property type="term" value="F:GTP 3',8'-cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01419">
        <property type="entry name" value="MoaC_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.640">
        <property type="entry name" value="Molybdopterin cofactor biosynthesis C (MoaC) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01224_A">
        <property type="entry name" value="MoaC_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023047">
        <property type="entry name" value="Mo_CF_biosynth-C_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023045">
        <property type="entry name" value="MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036522">
        <property type="entry name" value="MoaC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050105">
        <property type="entry name" value="MoCo_biosynth_MoaA/MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002820">
        <property type="entry name" value="Mopterin_CF_biosynth-C_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00581">
        <property type="entry name" value="moaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006870">
        <property type="entry name" value="PRK09364.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008999">
        <property type="entry name" value="PRK12343.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960:SF0">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SYNTHESIS PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01967">
        <property type="entry name" value="MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55040">
        <property type="entry name" value="Molybdenum cofactor biosynthesis protein C, MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 3" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molybdopterin cofactor biosynthesis C (MoaC)" evidence="4">
        <location>
            <begin position="13"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="73"/>
            <end position="75"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="109"/>
            <end position="110"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="153" mass="16859" checksum="595986221C362D7A" modified="2017-09-27" version="1">MELTHVDEKGVKMVEVGHKSEVFRKAVAKGRIYLRPETIELIKAGKTKKGNVIATAQIAGILAVKKTPELIPLCHPIPLTGVDISFEFGEDYIEATCEVRATYKTGVEMEALTGVSVALLTIWDMVKAVEKDKNGQYPVTRIENIHVVEKVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8V0</accession>
    <name>A0A218P8V0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS</fullName>
            <shortName evidence="6">tRNA(Ile2)-agm2C synthetase</shortName>
            <ecNumber evidence="6">6.3.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">tRNA(Ile2) agmatidine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tiaS</name>
        <name evidence="11" type="ORF">A3L08_07615</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">ATP-dependent agmatine transferase that catalyzes the formation of 2-agmatinylcytidine (agm2C) at the wobble position (C34) of tRNA(Ile2), converting the codon specificity from AUG to AUA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>cytidine(34) in tRNA(Ile2) + agmatine + ATP + H2O = 2-agmatinylcytidine(34) in tRNA(Ile2) + AMP + 2 phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43608"/>
            <dbReference type="Rhea" id="RHEA-COMP:10625"/>
            <dbReference type="Rhea" id="RHEA-COMP:10626"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58145"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:83545"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TiaS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.22" evidence="6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854447.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8V0"/>
    <dbReference type="GeneID" id="33316126"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07615"/>
    <dbReference type="OrthoDB" id="39189at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002101">
        <property type="term" value="P:tRNA wobble cytosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04482">
        <property type="entry name" value="RPA2_OBF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01892">
        <property type="entry name" value="tRNA_Ile2_agm2C_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053434">
        <property type="entry name" value="TiaS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053870">
        <property type="entry name" value="TiaS-like_TCKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013696">
        <property type="entry name" value="TiaS_FLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024913">
        <property type="entry name" value="tRNA_Ile2__agm2C_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055394">
        <property type="entry name" value="Zn_ribbon_TiaS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040849">
        <property type="entry name" value="tRNAmod_TiaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40705">
        <property type="entry name" value="TRNA(ILE2) 2-AGMATINYLCYTIDINE SYNTHETASE TIAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40705:SF1">
        <property type="entry name" value="TRNA(ILE2) 2-AGMATINYLCYTIDINE SYNTHETASE TIAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08489">
        <property type="entry name" value="TiaS_FLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22641">
        <property type="entry name" value="TiaS_TCKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23783">
        <property type="entry name" value="Zn_ribbon_TiaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TiaS-like TCKD" evidence="9">
        <location>
            <begin position="5"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="TiaS FLD" evidence="8">
        <location>
            <begin position="140"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="OB" evidence="7">
        <location>
            <begin position="270"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="TiaS C-terminal zinc ribbon" evidence="10">
        <location>
            <begin position="354"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ07196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="424" mass="48412" checksum="D06F03B335E83B5C" modified="2017-09-27" version="1">MLIHIGIDDTDSPNGMCTTYLGALLYRELSRIAEPLDLPRLIRLNPNVPYKTRGNGAVTMSFDADEGVIPEIKDTVLFYVSQLSDFEHENTNPGVVFFEGEIPDELREFSLKALREHVTIQEAEKVAREVGAEYFKFKLGRGVIGSLAAIGYPLERFTYELLAYREPENWGTPRRVNSESVFRVDKWAYPFSYDNVDPYKRSVLITPHGKDPVLVGIRGIDKTKVIQVFERVEFGEPVAFYQLYKTNQNTDDHLAPKKIGELKLYDSAVVRGRVVGEYWERGRHVFFELEDDTGRIRVAAFEPTKKFRNYVRKLLPGDEIIAAGGVKEHEGVLTLNLEKFYPVKLVPKVEYQKPKCPKCGGTMKSKGDYLKCKHCGYKMPKKLIPVEVLRDLEKKIYEVPPDARKHLSRPLVLPGGEDKLLELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8W8</accession>
    <name>A0A218P8W8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_07745</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854466.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8W8"/>
    <dbReference type="GeneID" id="33316151"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07745"/>
    <dbReference type="OrthoDB" id="87732at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000589">
        <property type="entry name" value="ABC transporter, ATP-binding subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050763">
        <property type="entry name" value="ABC_transporter_ATP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711:SF18">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="4"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="283" mass="32215" checksum="3D21FC5EDE3830AF" modified="2017-09-27" version="1">MPVIEVENVRKYYGDVRGVDGLSFSVEEGEIYGFLGPNGAGKTTTVKILVKIIKDYEGTVKVFGKDLREWGKDYYNKIGVSFEFPAVYSRLTALENLQFFASFYKRHLDPLEVLKMVGLEKEADQLVSGFSKGMKKKLDLARALLPDPEVLFLDEPLEGLDPASARKIKDLLLEMRENGKTVFLTTHNMYVADELCDRVAFIVEGKIALVDSPGELKVKMGKRLVRVEYVDDNDVKMAEFPLENLGRNEEFLNIIKNHEVRRINTEEPTLEEIFLKVTGRRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Z5</accession>
    <name>A0A218P8Z5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Putative nickel-responsive regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_08000</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Transcriptional regulator.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
        <text evidence="8">Binds 1 nickel ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the transcriptional regulatory CopG/NikR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854515.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Z5"/>
    <dbReference type="GeneID" id="33316205"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08000"/>
    <dbReference type="OrthoDB" id="25654at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010045">
        <property type="term" value="P:response to nickel cation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22231">
        <property type="entry name" value="RHH_NikR_HicB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="entry name" value="ACT-like. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="entry name" value="Met repressor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00476">
        <property type="entry name" value="NikR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050192">
        <property type="entry name" value="CopG/NikR_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022988">
        <property type="entry name" value="Ni_resp_reg_NikR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014864">
        <property type="entry name" value="TF_NikR_Ni-bd_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001884">
        <property type="entry name" value="PRK00630.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002169">
        <property type="entry name" value="PRK01002.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002815">
        <property type="entry name" value="PRK02967.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003381">
        <property type="entry name" value="PRK04460.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34719">
        <property type="entry name" value="NICKEL-RESPONSIVE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34719:SF2">
        <property type="entry name" value="NICKEL-RESPONSIVE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08753">
        <property type="entry name" value="NikR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8" id="KW-0533">Nickel</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Ribbon-helix-helix protein CopG" evidence="9">
        <location>
            <begin position="5"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor NikR nickel binding C-terminal" evidence="10">
        <location>
            <begin position="55"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ07267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="138" mass="15724" checksum="CAEAC8DD75DAE131" modified="2017-09-27" version="1">MGVIRFGVSVPEELLEKFDRIIEEKGYVNRSEAIRDMMRDFIVRHEWEQGDAEVAGTITLLYNPDEVDVVKELLDLQHEYIDEVVSSLHVHMDEHNCLEVVIVKGKASRIKEIADRLLSLKGVKHGKLVMTGTGKEPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P903</accession>
    <name>A0A218P903_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MBL fold metallo-hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07975</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854510.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P903"/>
    <dbReference type="GeneID" id="33316200"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07975"/>
    <dbReference type="OrthoDB" id="6433at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07709">
        <property type="entry name" value="flavodiiron_proteins_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045761">
        <property type="entry name" value="ODP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016440">
        <property type="entry name" value="Rubredoxin-O_OxRdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43717">
        <property type="entry name" value="ANAEROBIC NITRIC OXIDE REDUCTASE FLAVORUBREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43717:SF1">
        <property type="entry name" value="ANAEROBIC NITRIC OXIDE REDUCTASE FLAVORUBREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19583">
        <property type="entry name" value="ODP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005243">
        <property type="entry name" value="ROO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="1">
        <location>
            <begin position="265"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="409" mass="46621" checksum="3B53731BC1D62DE7" modified="2017-09-27" version="1">MVEVRVEQLCTDPELYIIRIDDGKIRYFEATWDIPEGITYNSYLMKLKDAVVLFDTSKAEYADLFMDALKKLVDPKEITHIIVHHTEPDHSGALPKVLEENGYRAQLIGTTFARNLLQGFYGDKVVENFKVAKDGEEMDIGGKTFRFIAVPWLHWPDTMITYVVEDRLIFSCDAGGGYGIPETIDDSDEKVVEEYLPHVTKYIVTVIGHYHKYIVQNIKKLKELGIVESARMILPGHGLVWRKNPVRIFEHYERVGAGVPTKDKVLVIYDSMYGFVERRMNIVIDELKKLGKNPIVYRFTDKEVPAVSDILGEVPDSEALIIGASTYEADIHPRIRYALFEILDKANYEKPVLVVGAFGWGGVAGKKIETLISRSKFDLVDTVESRGMPTQEDEERLREGVRKLVSWIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P918</accession>
    <name>A0A218P918_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Anion transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_08120</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_232461707.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P918"/>
    <dbReference type="GeneID" id="33316229"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08120"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051475">
        <property type="entry name" value="Diverse_Ion_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43568">
        <property type="entry name" value="P PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43568:SF1">
        <property type="entry name" value="P PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03600">
        <property type="entry name" value="CitMHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="308"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="345"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Citrate transporter-like" evidence="7">
        <location>
            <begin position="20"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="363" mass="40105" checksum="36738E55261DE15F" modified="2017-09-27" version="1">MARGLKEFIRREWFLSSLLVLYVVLLLRDGSLLKRTPALIDWGGLSLITSLILVSRGLELSGAFMRLSLQIIALSRGSERRLALILLPAIALSSAIIMNDTAMLIFIPFIVALSRLIERDMTALVVFSAIAANVGSALTPIGNPQNIIIWRYYGVPFPDFVAYMLPFVLLWLILLLLFAFVTFRGSLPERKLHQVKVREGLLWSSLFLLIANIVLAREGKALWTLPLTVGVLFLVGRDVLLSFDWALVLTFAFIFVDFGELSEIMASLGLHPPSSEIGLFISSALLSQVVSNVPATVLLLSAENWGPLAVGVNAGGTGFMVGSLANLIAIRIARMSIRKFHRYSVPYFLLILPLSTLFLWILP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P919</accession>
    <name>A0A218P919_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="18">ATPase P</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">A3L08_08015</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854517.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P919"/>
    <dbReference type="SMR" id="A0A218P919"/>
    <dbReference type="GeneID" id="33316208"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08015"/>
    <dbReference type="OrthoDB" id="8588at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043682">
        <property type="term" value="F:P-type divalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055070">
        <property type="term" value="P:copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd02094">
        <property type="entry name" value="P-type_ATPase_Cu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000005">
        <property type="entry name" value="Copper-exporting P-type ATPase A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000002">
        <property type="entry name" value="Copper-transporting ATPase 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006122">
        <property type="entry name" value="HMA_Cu_ion-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01511">
        <property type="entry name" value="ATPase-IB1_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00003">
        <property type="entry name" value="copper ion binding protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520">
        <property type="entry name" value="ATP7, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520:SF8">
        <property type="entry name" value="P-TYPE CU(+) TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00943">
        <property type="entry name" value="CUATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0186">Copper</keyword>
    <keyword evidence="8" id="KW-0187">Copper transport</keyword>
    <keyword evidence="13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="163"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="186"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="252"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="403"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="430"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="745"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="775"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="17">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="17">
        <location>
            <begin position="71"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="575"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ASJ07269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="799" mass="86504" checksum="CAD43D8D499517E8" modified="2017-09-27" version="1">MEITLKVSGMTCAMCVKTIEMALKELPGVKDARANLGTETVLVKFDESEVGLNQIIKAIEDVGYTVVRERKDVVLKIGGMTCAMCVKTIEAALKELPGVLEASVNLGTETARVSYDPSVVDIEDIKKAIESVGYQFIGVEGEETHDVERELREKTLRDMKRKLAVAWGIGIALFASMQAGRFGLEIPYLMPLQFVLATLAIAYAGRGIFGKALSSLRHKNLTMEVMYSLGIGSAYFASVLATVGIIPKEFNFYEASVLLMAFLLLGRYLETRAKGRTSEAIKKLIGLQAKKATVLRDGEEVEVPISEVKVGDVVIVKPGERIPVDGIVLEGESYVDESMITGEPVPGLKREGDEVIGGTINKNSVLKIEAKRVGRDTVLAGIIRLVEEAQNTKPPIQRLADKIVTYFIPAVLTIALLSFAYWYFVAGKPLLFAFTTLLSVLVIACPCAFGLATPTALTVGMGKGAEMGILIKNGEVLEIARKATVVLFDKTGTLTRGQPEVTDVITFGMDEKELLKLVASAERRSEHPLGEAVVRKAEELGLELEEPEKFEAITGKGVRAVVSGREVLAGNRKLMVESGLNLDEVEETLRKLENEAKTAVIVAVDGKITGVLGIADTIKEGAKEVIEELHRMGKKVGMITGDNRRTAEAIGRVLGVDYVLAEVMPGEKANEVKKLQEKGEIVVFVGDGINDAPALAQADIGIAVGNATDIAMESGDIVLMRNDPRDVVRAIKLSQKTLGKIKQNIFWAMFYNTILIPFAAGLAFILFGIEFRPEWAAGAMSMSSVSVVTNSLLLKRAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P923</accession>
    <name>A0A218P923_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cytochrome C biogenesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_08175</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P923"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08175"/>
    <dbReference type="OrthoDB" id="115386at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051790">
        <property type="entry name" value="Cytochrome_c-biogenesis_DsbD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039447">
        <property type="entry name" value="UreH-like_TM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31272:SF9">
        <property type="entry name" value="BLL1027 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31272">
        <property type="entry name" value="CYTOCHROME C-TYPE BIOGENESIS PROTEIN HI_1454-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13386">
        <property type="entry name" value="DsbD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="205"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="270"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="312"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="348"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="399"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Urease accessory protein UreH-like transmembrane" evidence="3">
        <location>
            <begin position="209"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="176"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="432" mass="46015" checksum="26B2C9A6C31B6723" modified="2017-09-27" version="1">MRRALLIFMLSLIFTGFVSSGEISYGRLEFHDVSSEEELNALISAHEGEYFFIFYHSESCPACRYMKENVFPTQKAKEVLSGINLVAIDVYKGRGLTTLQYVVRGKVLVIQPENSGYYTPKNPGEKINVGVPGTPTMVLFKVENGEKVLKGVAVGALNPDGLEFFIKTAIGDELQNSTTNSTQTETAKEGASGEESSGTRLSLAVLLPIFSAGVLSVFSPCVLPVIAGALSLTFARRKVEAVIAGMVASFALLGALVGSLGSYASQIQGALYIIGGVGFILIGASFVSADLRARLERLLSFSPAEGIVSKKGIAYDFALGSALGATWLGCIAPYVGFAVITAALTGEILKGVVVMGTYGLGMGLTVYLITMSKDLGEWINRKLLSRGVSLGGKSSRWELALGLALIVLGMLMLTEVTPLKLWSKLFESLSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P929</accession>
    <name>A0A218P929_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Shikimate kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_08095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854530.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P929"/>
    <dbReference type="GeneID" id="33316224"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08095"/>
    <dbReference type="OrthoDB" id="9602at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="12789" checksum="026932CE7D0E4489" modified="2017-09-27" version="1">MEDVEKTPMVVLLPEERVLTESLRGRDFSVIAPYVEEAFELALRGGWKKALVLNGPVYSTYLGYNPEPIKIALELGAIPDLSGKGPAFFALAEEPENLIHEWNRFGEVMVTKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P933</accession>
    <name>A0A218P933_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_08225</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P933"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08225"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="67" mass="7512" checksum="4D12521291F29629" modified="2017-09-27" version="1">MRYYTPLPLKLCAILALRSQEKKRAIATTIPNPKKLLVPMNDAGAATLCGSFQSHNHPWATAKMRYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P965</accession>
    <name>A0A218P965_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_08375</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854582.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P965"/>
    <dbReference type="GeneID" id="33316281"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08375"/>
    <dbReference type="OrthoDB" id="103603at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="134" mass="14627" checksum="42150D9C92E17A64" modified="2017-09-27" version="1">MPRKVLALFLLGVLFFAGVAVALNGKEPEAITRDPLVHDENTYAPTEDGSLYCWISGYASQSQPGYLYNIYGNAGSTGVCDLWTKKFSPSTATPTGRIRLGLCPGGRHRLVPLLAQDHLRRDNLGSGNYLYKHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9B5</accession>
    <name>A0A218P9B5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Radical SAM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_08505</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854605.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9B5"/>
    <dbReference type="GeneID" id="33316307"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08505"/>
    <dbReference type="OrthoDB" id="17974at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040084">
        <property type="entry name" value="GTPase_Obg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43787">
        <property type="entry name" value="FEMO COFACTOR BIOSYNTHESIS PROTEIN NIFB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43787:SF11">
        <property type="entry name" value="UPF0026 PROTEIN SLR1464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01083">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="10"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="315" mass="36310" checksum="5004885D84DA6037" modified="2017-09-27" version="1">MIAFGPVPSRRLGKSLGVNNIPDKVCSFACVYCQIGKTIRMELERRPFYEPELIFKEVRKKVEEARERGERIDYITFVPDGEPTLDANLGREAELLKTLGIPLAILTNSSLIWREDVREDLLKFDFVSLKVDAVSENLWRRIDRPHKSLSLERILEGMLEFRKTFRGKVVTETMLIDGIDYGNEFEEIAEFLAELKPDVAYIAIPTRPPAEGWVKPAGEDVINHAFQTFVEKLGEDRVEYLIGYEGNAFAFTGNVENDLLSITSVHPMREDAVDEFLRKANADWSVVERLLREGKLIELEYNGKKFYMRRLKSRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9F1</accession>
    <name>A0A218P9F1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="14">DNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">A3L08_08745</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HerA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854645.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9F1"/>
    <dbReference type="GeneID" id="33316355"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08745"/>
    <dbReference type="OrthoDB" id="107033at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008571">
        <property type="entry name" value="HerA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018538">
        <property type="entry name" value="HerA_barrel_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033186">
        <property type="entry name" value="HerA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957:SF1">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09378">
        <property type="entry name" value="HAS-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05872">
        <property type="entry name" value="HerA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 14" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase HerA barrel" evidence="13">
        <location>
            <begin position="7"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase HerA central" evidence="11">
        <location>
            <begin position="139"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase HerA-like C-terminal" evidence="12">
        <location>
            <begin position="401"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF05872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF09378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ07399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="556" mass="62571" checksum="708E3EAE4E119850" modified="2017-09-27" version="1">MERDSPVGIVFGESTTDHFTFIVNPRNELPRFGEFLIARNRDGDEVLALLKSIRNVNWLMDAGRGSYDYVEKTVNVFSKGVLDKSEEILATAKVLGVLRTADGDFLPKPAPNRVPIKPGEKVYLARDEDLERIFSQGHIRLGRLIAREKVEVNLDANRLVSRHFAVLAVTGAGKSNTIAVLTKELVSNINATVVILDPHGEYRHLSWPGARVNPIKATIDPGRIRLSEFATLLGIAENASLQRRFLGLVYRTVREEMRRNGRVVGGMDFIHAMEDKIEEWIRVYENTDDKIIYYYDEKGIETPRKIQAKDLDSLIRLKDYLSELRANFGEFISPVNVLSEIRPGMVNVIDLSGMEEEQMITLASFVLRGILKNRIEYIKALRTNDRPTAREIIENYPAVRKPLLVIVEEAHIFAPRGEKNPATLWLGKIAREGRKFGVGLGIVSQRPKKLDDDILSQTNTKIILKLVEPNDQRYVQQASEQISEDLLSDIASLGVGEAVIVGYAITIPAMVKIYSFERDMKGHYGGGDIDIVAEWLEGKDEEENVTEEEAINALPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9F9</accession>
    <name>A0A218P9F9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_08765</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9F9"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08765"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.410">
        <property type="entry name" value="Protein of unknown function UPF0759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002763">
        <property type="entry name" value="DUF72"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036520">
        <property type="entry name" value="UPF0759_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30348:SF4">
        <property type="entry name" value="DUF72 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30348">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YECE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01904">
        <property type="entry name" value="DUF72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117396">
        <property type="entry name" value="TM1631-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="62" mass="7344" checksum="0323DDCF6C5061F1" modified="2017-09-27" version="1">MIRVGTCGFCEGRGKYYRDFDAVEVQQTFYRILREKTLKRWRKEAPEGFTFSIKAFQGAEDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9H0</accession>
    <name>A0A218P9H0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Iron ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_08980</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854687.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9H0"/>
    <dbReference type="GeneID" id="33316402"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08980"/>
    <dbReference type="OrthoDB" id="28023at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30183">
        <property type="entry name" value="MOLYBDENUM TRANSPORT SYSTEM PERMEASE PROTEIN MODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30183:SF3">
        <property type="entry name" value="MOLYBDENUM TRANSPORT SYSTEM PERMEASE PROTEIN MODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="98"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="290"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="339"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="430"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="465"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="518"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="579"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="98"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="394"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ07442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="612" mass="67306" checksum="F7D269377ADDE519" modified="2017-09-27" version="1">MKVSKWSERLFGTPIFDPVVTTSFLFPLLYLIAFLIIPVLAMLTIAFVYNGHISLHWFSNILKSDYYISRPTGEFYRLITMPSGEKIYYVQGVDFGVILNSIIVSISVMILTTILGTVFAFVMARYDFPGKNIVRILLFVPLLVTPFVNVFIVKKMFLPDGIINWLFYDLLHLFPHRIVIDGLVGVIVAQSLTYYPIVYLNAYASFINIDPTLEEQAENLGSGGFHLFRTVTFPLALPGIAAGATLVGIFSLEDLAAPIVFQGNPLARKLMSFQIYSAFTSGFNVGSPQLAALAIIMLTIAILMFLGIRKYVSMRQYAMLSKGGRWKPRVAKPKGWQAALIYLVVLPLLLLSIFPQVGVVLLAFSESWSGTWPDGFTTQHIMSIITQPDIERVIMNSVMYSTVAIVVIILLSLTASYASSRFKKSQLGPVLDSLATIPIAVPGIVIAMGYFFFFAKVFPNTPLDPTNLLGFNPAMVLVLAYSIRRLPFAARSISAGIQQVHVSLEEAALNLGASRWKALTGILIPLILLNLLGGAMLSFVYCMSETSVGITLGSINPDYYPITARMVELMTSAVGSANLAAALGVFLMTVQIIAIVLANVITKQRYSFIGLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9L5</accession>
    <name>A0A218P9L5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854718.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9L5"/>
    <dbReference type="GeneID" id="33316437"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09155"/>
    <dbReference type="OrthoDB" id="85992at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="106" mass="12812" checksum="3B0E3E52C0F31F5E" modified="2017-09-27" version="1">MSLKTLVKLYRVAKGKEKVAIAWKLVHEAARYSNHEPYWEFLKENFEVRADEIKDVLRFLEGRGELQIKRSIDGKRLYVSTLKDIRENPVRLDRWLRLTSRRRPMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9N6</accession>
    <name>A0A218P9N6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sugar fermentation stimulation protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">sfsA</name>
        <name evidence="4" type="ORF">A3L08_09250</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SfsA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854732.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9N6"/>
    <dbReference type="GeneID" id="33316456"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09250"/>
    <dbReference type="OrthoDB" id="34139at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22358">
        <property type="entry name" value="SfsA-like_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00095">
        <property type="entry name" value="SfsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005224">
        <property type="entry name" value="SfsA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040452">
        <property type="entry name" value="SfsA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041465">
        <property type="entry name" value="SfsA_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00230">
        <property type="entry name" value="sfsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30545">
        <property type="entry name" value="SUGAR FERMENTATION STIMULATION PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30545:SF2">
        <property type="entry name" value="SUGAR FERMENTATION STIMULATION PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03749">
        <property type="entry name" value="SfsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17746">
        <property type="entry name" value="SfsA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SfsA N-terminal OB" evidence="3">
        <location>
            <begin position="17"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar fermentation stimulation protein C-terminal" evidence="2">
        <location>
            <begin position="86"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="241" mass="27152" checksum="CEC8CD18FFF20550" modified="2017-09-27" version="1">MRKPVLLELNVVPCTFIERLNRFVALVDVKGETRKALITNTGRLEEFMTPGRKAFCTPKSGGKTDFVLVAFEDLGGRGAVIDTRTQAKAFERALELNLVPWLKDCRIKRKEVRTGKSRLDYLFECPDGEIHAEMKSAVLRGGDRGEYAMYPDCPSTRGQKHIRELIGLSKEGKRTMIFFIGAMPGVEKFTPYEKGDPEIARLLKEARKAGVEIHALSISLLPDGRVVLERPSLEVELREDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Q1</accession>
    <name>A0A218P9Q1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Septum site-determining protein MinD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_09365</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854755.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Q1"/>
    <dbReference type="GeneID" id="33316479"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09365"/>
    <dbReference type="OrthoDB" id="31168at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051782">
        <property type="term" value="P:negative regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000285">
        <property type="entry name" value="Sporulation initiation inhibitor Soj"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010224">
        <property type="entry name" value="MinD_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025501">
        <property type="entry name" value="MinD_FleN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050625">
        <property type="entry name" value="ParA/MinD_ATPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01969">
        <property type="entry name" value="minD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384:SF10">
        <property type="entry name" value="ATPASE INVOLVED IN CHROMOSOME PARTITIONING, PARA_MIND FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384">
        <property type="entry name" value="SEPTUM SITE-DETERMINING PROTEIN MIND HOMOLOG, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003092">
        <property type="entry name" value="MinD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="4">
        <location>
            <begin position="3"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="11"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR003092-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="245" mass="26210" checksum="95623882020324BE" modified="2017-09-27" version="1">MGRLISVASGKGGTGKTTTTANLAIALGKMGYRVCAVDADLTMANLSLVMGIDDADTTIHDVLAGMADINDAIYATTYENVHVIPAAIDWEHVIRADPRKLPDTIKPLKEKFDFVIIDSPAGLQMDAMNAMMSGEEVLLVTNPEISCLTDTMKVGMVLKRAGLAVLGFVLNRYGRSENDIPPDAAEEVMEIPLLEVIPEDPKVREATLEGVPVVEYAPNSPGAKAFMELAETIVRIAGLKARVMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Q3</accession>
    <name>A0A218P9Q3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">acetate--CoA ligase (ADP-forming)</fullName>
            <ecNumber evidence="2">6.2.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_09315</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP + CoA = acetyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15081"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="6.2.1.13" evidence="2"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854745.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Q3"/>
    <dbReference type="GeneID" id="33316469"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09315"/>
    <dbReference type="OrthoDB" id="18103at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043758">
        <property type="term" value="F:acetate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051538">
        <property type="entry name" value="Acyl-CoA_Synth/Transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043938">
        <property type="entry name" value="Ligase_CoA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032875">
        <property type="entry name" value="Succ_CoA_lig_flav_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43334:SF1">
        <property type="entry name" value="3-HYDROXYPROPIONATE--COA LIGASE [ADP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43334">
        <property type="entry name" value="ACETATE--COA LIGASE [ADP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19045">
        <property type="entry name" value="Ligase_CoA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13607">
        <property type="entry name" value="Succ_CoA_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA-binding" evidence="6">
        <location>
            <begin position="12"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="473" mass="51667" checksum="E40CF762BAC799B1" modified="2017-09-27" version="1">MAERIVEEMRPFFDPKAVAIIGATNKKGKVGNVIFENFRMNKERGIFKGNIYPVNPKLDEIEGYKVYKSVAELPDDTDLAVISIPAPFVPQTMRDIAEKGIKAVIIITGGFGELGEEGKKLEREIYEIAKANGIRVIGPNCVGVYVPDTGVDTVFLPEEKMDRPKSGPIAFVSQSGAFAAAMLDWAAMAGIGIGKMVSYGNKLDVDDADLMDYFIHDDEINVVTFYIEGVKDGRKFMEAAKRITKVKPVIALKSGRTEYGAKAASSHTGSLAGADTIYDAVFKQTGIIRAEDFEHMFDLAKAFAALKDKLPKGDRIGIITDGGGAGVMASDAVAKFGLKMADLSEETIKFLKENFPPHAVPGNPTDVVGDTDAERYRVAIEAFTKDPNVDAIVIIVLFQVPLLEEEKIIDILAEYQKKSDKPIVAVAMGGKKTDHYARILEDKGVPVYPTPERGVRAMAGLVKYAEYLKRLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9R0</accession>
    <name>A0A218P9R0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">protein adenylyltransferase</fullName>
            <ecNumber evidence="9">2.7.7.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">A3L08_09405</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-tyrosyl-[protein] + ATP = O-(5'-adenylyl)-L-tyrosyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:54288"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="EC" id="2.7.7.108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-(5'-adenylyl)-L-tyrosyl-[protein] + ATP = O-[5'-(adenylyl-(5'-&gt;3')-adenylyl)]-L-tyrosyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:66528"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="Rhea" id="RHEA-COMP:17046"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="ChEBI" id="CHEBI:167160"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the MntA antitoxin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.108" evidence="9"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854761.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9R0"/>
    <dbReference type="GeneID" id="33316487"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09405"/>
    <dbReference type="OrthoDB" id="9287at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070733">
        <property type="term" value="F:AMPylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05403">
        <property type="entry name" value="NT_KNTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009185">
        <property type="entry name" value="Nucleotidl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052038">
        <property type="entry name" value="Type-VII_TA_antitoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33571:SF14">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE MJ0435-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33571">
        <property type="entry name" value="SSL8005 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005928">
        <property type="entry name" value="Nucleotidltrnsf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-1277">Toxin-antitoxin system</keyword>
    <keyword evidence="3 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="13">
        <location>
            <begin position="45"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ07517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="232" mass="27289" checksum="E6AB0A3D8D055C07" modified="2017-09-27" version="1">MPREKVVRVWDEREVVYTPKRWRYLREKREKALGIMERLEQFDPQLYGSVARGDVRRDSDIDVFIPYRVPSYLIELALEGLVGRRKIVMATPWHLIKGVIEIDEETTVTFPLIDPTDRELEFYRWGGAIDLWGVKTNERVPGVNKKLILIIPTERGHIEREVVGRESEVAKLLGVSIDIVTERVHVLTRRDRIGRTGIYIDEEVPDWMTFEEALKMIADRDPNVRRKIRERG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9T9</accession>
    <name>A0A218P9T9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase</fullName>
            <shortName evidence="5">HPPK</shortName>
            <ecNumber evidence="5">2.7.6.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">6-hydroxymethyl-7,8-dihydropterin diphosphokinase</fullName>
            <shortName evidence="5">6-HMPDK</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">7,8-dihydro-6-hydroxymethylpterin diphosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">7,8-dihydro-6-hydroxymethylpterin pyrophosphokinase</fullName>
            <shortName evidence="5">PPPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mptE</name>
        <name evidence="7" type="ORF">A3L08_09565</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the transfer of diphosphate from ATP to 6-hydroxymethyl-7,8-dihydropterin (6-HMD), leading to 6-hydroxymethyl-7,8-dihydropterin diphosphate (6-HMDP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>6-hydroxymethyl-7,8-dihydropterin + ATP = (7,8-dihydropterin-6-yl)methyl diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal 6-HMPDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.3" evidence="5"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854792.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9T9"/>
    <dbReference type="GeneID" id="33316519"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09565"/>
    <dbReference type="OrthoDB" id="34207at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003848">
        <property type="term" value="F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004788">
        <property type="term" value="F:thiamine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02131">
        <property type="entry name" value="HMPDK_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027510">
        <property type="entry name" value="HMPDK_MptE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002826">
        <property type="entry name" value="MptE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036759">
        <property type="entry name" value="TPK_catalytic_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39648">
        <property type="entry name" value="6-HYDROXYMETHYL-7,8-DIHYDROPTERIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39648:SF1">
        <property type="entry name" value="6-HYDROXYMETHYL-7,8-DIHYDROPTERIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01973">
        <property type="entry name" value="MptE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63999">
        <property type="entry name" value="Thiamin pyrophosphokinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="6-hydroxymethylpterin diphosphokinase MptE-like" evidence="6">
        <location>
            <begin position="49"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="235" mass="26359" checksum="1BB045BE1269B7FC" modified="2017-09-27" version="1">MNWEQWKPFYLRIVREMGYSIDGDRRAAQILRALLLEGDEYILREELEAIIRKKAYVFGAGPSLEDALKQFDFSDGTLIAADGATSALLDAGLVPDVIVTDLDGRIPDLRIANDRGSFMAIHAHGDNIDKLTTYVPFFSRILGTCQTDPLDIVYNFGGFTDGDRAAFLAEALGAREIVLIGFDFGDVVGKWSKPSLREHSPVWESKGKKFAFAQELLRWLEKNGKARIDYLRPDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9W1</accession>
    <name>A0A218P9W1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleotidyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854802.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9W1"/>
    <dbReference type="GeneID" id="33316530"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09620"/>
    <dbReference type="OrthoDB" id="96853at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07749">
        <property type="entry name" value="NT_Pol-beta-like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019646">
        <property type="entry name" value="Aminoglyc_AdlTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10706">
        <property type="entry name" value="Aminoglyc_resit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="18366" checksum="DD91AB2A126B800F" modified="2017-09-27" version="1">MVPESHLKVLRKLYERLKDSDVDWAVTGSLGFALQGVPVEPHDIDIQTDKEGAYEIERLFSEFVVRPVKFRESERIRSHFGALRIDGIEVEIMGDIQKKVGDEWEPPVDVRKYRRFVEIEGMKIPVLDLEYEYQAYLKLGRVEKAEILKRFLEGRSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9X4</accession>
    <name>A0A218P9X4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">MazF family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09795</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854834.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9X4"/>
    <dbReference type="GeneID" id="33316565"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09795"/>
    <dbReference type="OrthoDB" id="90963at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003477">
        <property type="entry name" value="PemK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011067">
        <property type="entry name" value="Plasmid_toxin/cell-grow_inhib"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33988">
        <property type="entry name" value="ENDORIBONUCLEASE MAZF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02452">
        <property type="entry name" value="PemK_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50118">
        <property type="entry name" value="Cell growth inhibitor/plasmid maintenance toxic component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="124" mass="14256" checksum="1FED13D922492EDD" modified="2017-09-27" version="1">MSRRCEAPRGLRQWEVVLLEFPFSDSPKKKLRPVLVLSNSDFNRISNSVVVCQITSNLSSGFKEYNVFIEPGDVCLYGDAFMRPSLVKPYMVFSVSKREVRFKVGVLSEEKADEVKEKLKKLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9X6</accession>
    <name>A0A218P9X6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09675</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854812.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9X6"/>
    <dbReference type="GeneID" id="33316541"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09675"/>
    <dbReference type="OrthoDB" id="102487at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="79" mass="9329" checksum="FB3C462EEB062B2E" modified="2017-09-27" version="1">MNVRPFLKFLFFHLPRILFQIAGLFRVINQGKRAFRKGLESEDLPKELVEELLGEFDPLEDLSLREVLRFPMGRKRGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Y0</accession>
    <name>A0A218P9Y0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Molybdate/tungstate transport system permease protein WtpB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">A3L08_09785</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Part of the ABC transporter complex WtpABC involved in molybdate/tungstate import. Probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The complex is composed of two ATP-binding proteins (WtpC), two transmembrane proteins (WtpB) and a solute-binding protein (WtpA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854832.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Y0"/>
    <dbReference type="GeneID" id="33316563"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09785"/>
    <dbReference type="OrthoDB" id="11163at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053405">
        <property type="entry name" value="Mo/W_ABC_Transporter_Permease"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040839">
        <property type="entry name" value="tungstate_WtpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30183">
        <property type="entry name" value="MOLYBDENUM TRANSPORT SYSTEM PERMEASE PROTEIN MODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30183:SF3">
        <property type="entry name" value="MOLYBDENUM TRANSPORT SYSTEM PERMEASE PROTEIN MODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="90"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="52"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ07589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="248" mass="26959" checksum="6DFD47B53528B590" modified="2017-09-27" version="1">MRRDYTLYLFATIGSFLVVYIALPLVVIFLKQAADLRMLIKTLHDPLVIESLRNSLLTATATALIALLFGVPLGYVLARKEFPGKSLVQALVDVPIVIPHSVVGIMLLVTFSNAILDSYTGIIAAMLFVSAPFTINAARDGFLAVDEKLEQVARTLGASRLRAFSSIALPMAFPAIASGAIMTWARAISEVGAILIVAYYPKTAQVLVMEYFNNYGLRASRPISVILVAMSLAIFVLLRWLVGRAKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Z2</accession>
    <name>A0A218P9Z2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CAAX prenyl protease 2/Lysostaphin resistance protein A-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_09630</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854804.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Z2"/>
    <dbReference type="GeneID" id="33316532"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09630"/>
    <dbReference type="OrthoDB" id="137554at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080120">
        <property type="term" value="P:CAAX-box protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="2">
        <location>
            <begin position="103"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="257" mass="29011" checksum="E3A937BD04AB3C26" modified="2017-09-27" version="1">MDRGKKIALSALGVEIVGSSILLLFYRDIGWVPLGILMLFLLYLVVKKLGVGKEELGLGGELDLKIHILLPIAFMLLSFVWILPLGVGLNTFGKPLKVVLLIYLVLLVRYLVFVALYEEILFRGLMQRGFELWKGPKFAVIATAIIFSLGHIGSRFSFQPTFPNFWRLYNPLLAGIVYSIYRWKFRRIEGLILAHGLSDFFDRIMTVEKADWLLGTLEGHVYMVAAYTAVMALEGYAYVKIAEKLGETAKKSKEMVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Z8</accession>
    <name>A0A218P9Z8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">A3L08_02415</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854869.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Z8"/>
    <dbReference type="GeneID" id="33315088"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02415"/>
    <dbReference type="OrthoDB" id="33422at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002144">
        <property type="term" value="C:cytosolic tRNA wobble base thiouridylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01993">
        <property type="entry name" value="TtuA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000174">
        <property type="entry name" value="ATPase, PP-loop superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000541">
        <property type="entry name" value="Ncs6/Tuc1/Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054306">
        <property type="entry name" value="TtuA-like_LIM_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00269">
        <property type="entry name" value="TIGR00269 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807">
        <property type="entry name" value="ATPASES OF THE PP SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807:SF27">
        <property type="entry name" value="TRNA-5-METHYLURIDINE(54) 2-SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22082">
        <property type="entry name" value="TtuA_LIM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Rhodanese" evidence="13">
        <location>
            <begin position="14"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="3"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR004976-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ07626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="304" mass="35043" checksum="75C4554AD780C40F" modified="2017-09-27" version="1">MKCKFCDKLAFIKLHYPKMYLCEEHFTEYFERKVKRTIERYKMFKPDDKILVVVSGGKDSAVTAYVLKKFGYNIECLHINLGIGEYSEKGERYAKKQCEMIGAPLHIVRVRELLGAGIGEVRTRRPTCSYCGLTKRYIFNKFAYDNGFDVVATGHNLDDEASFIFNNIMNWNTQYLAKQGPVTPSALSGKLVKKVKPLYEVTEREVVAYALANGIEYEIDECPHARGATTLEWKAILNEMEEKRPGTKINFVKGYLRKKHLFEAELEEAELKECKVCGMPSSGEVCSFCRFWRLEKPVDFKIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA14</accession>
    <name>A0A218PA14_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleotide pyrophosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_02320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA14"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02320"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="27946" checksum="F2A9D015FEC3696F" modified="2017-09-27" version="1">MLTGRKIPEIEEPFIKRQKFIANKGNITKVKTPWYVKLGAKILPLSIRKRIGDAMTPDPFKETYDYLLKTRKYRTIFDYFEKTWTNGVPSYGRNVSTPEIKEAMYKAVKGNLKPLLEYAFRTYETDRKALFEALEGDYELIFWYTPFLDEISHFLIRKKLKLMNVYFDLNKLVKNVSEKLDEDDVLYIISDHGMEPIPGDPRGGDHSDHGFFSSNTGETIKKPQDLFELVVSKSRAYYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA18</accession>
    <name>A0A218PA18_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">S-adenosylmethionine decarboxylase proenzyme</fullName>
            <shortName evidence="15">AdoMetDC</shortName>
            <shortName evidence="15">SAMDC</shortName>
            <ecNumber evidence="15">4.1.1.50</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="15">S-adenosylmethionine decarboxylase beta chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="15">S-adenosylmethionine decarboxylase alpha chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="15" type="primary">speH</name>
        <name evidence="16" type="ORF">A3L08_02550</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 15">Catalyzes the decarboxylation of S-adenosylmethionine to S-adenosylmethioninamine (dcAdoMet), the propylamine donor required for the synthesis of the polyamines spermine and spermidine from the diamine putrescine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 15">
            <text>S-adenosyl-L-methionine + H(+) = S-adenosyl 3-(methylsulfanyl)propylamine + CO2</text>
            <dbReference type="Rhea" id="RHEA:15981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="4.1.1.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="15">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 15">Amine and polyamine biosynthesis; S-adenosylmethioninamine biosynthesis; S-adenosylmethioninamine from S-adenosyl-L-methionine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 15">Heterotetramer of two alpha and two beta chains arranged as a dimer of alpha/beta heterodimers.</text>
    </comment>
    <comment type="PTM">
        <text evidence="15">Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl group blocking the N-terminus of the alpha chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14 15">Belongs to the prokaryotic AdoMetDC family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.50" evidence="15"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854870.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA18"/>
    <dbReference type="GeneID" id="33315116"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02550"/>
    <dbReference type="OrthoDB" id="114016at2157"/>
    <dbReference type="UniPathway" id="UPA00331">
        <property type="reaction ID" value="UER00451"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004014">
        <property type="term" value="F:adenosylmethionine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.110:FF:000001">
        <property type="entry name" value="S-adenosylmethionine decarboxylase proenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.110">
        <property type="entry name" value="S-adenosylmethionine decarboxylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00464">
        <property type="entry name" value="AdoMetDC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042286">
        <property type="entry name" value="AdoMetDC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003826">
        <property type="entry name" value="AdoMetDC_fam_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042284">
        <property type="entry name" value="AdoMetDC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016067">
        <property type="entry name" value="S-AdoMet_deCO2ase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017716">
        <property type="entry name" value="S-AdoMet_deCOase_pro-enz"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03330">
        <property type="entry name" value="SAM_DCase_Bsu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33866">
        <property type="entry name" value="S-ADENOSYLMETHIONINE DECARBOXYLASE PROENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33866:SF2">
        <property type="entry name" value="S-ADENOSYLMETHIONINE DECARBOXYLASE PROENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02675">
        <property type="entry name" value="AdoMet_dc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56276">
        <property type="entry name" value="S-adenosylmethionine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="4 15" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="9 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 15" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="11 15" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="10 15" id="KW-0704">Schiff base</keyword>
    <keyword evidence="6 15" id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword evidence="8 15" id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_5023309986" description="S-adenosylmethionine decarboxylase beta chain" evidence="15">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023309987" description="S-adenosylmethionine decarboxylase alpha chain" evidence="15">
        <location>
            <begin position="63"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid" evidence="15">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for processing activity" evidence="15">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for catalytic activity" evidence="15">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autolysis" evidence="15">
        <location>
            <begin position="62"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="15">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ASJ07627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="139" mass="15642" checksum="5D4BCDFCFF8B0F3B" modified="2017-09-27" version="1">METIGFHYVVEAAGCDPEVLSNADKIRQIFLEAAKVSNMEVKSSYFFRFSPTGVSGVVIVAESHISVHTWPEKGYAALDVYTCGTKADPEKAVDYILEKFRAKYAHVSEIKRGIEEDDETYTHMIMTWEEALRKNGNGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA37</accession>
    <name>A0A218PA37_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TIGR04140 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_02285</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854866.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA37"/>
    <dbReference type="GeneID" id="33315062"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02285"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026486">
        <property type="entry name" value="CHP_AF_0576"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04140">
        <property type="entry name" value="chp_AF_0576"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="65" mass="7476" checksum="EDC58CE3C84318CF" modified="2017-09-27" version="1">MRIIETPIPLKELNEIRERGGAKVSLTLLGRTERNGIPLNRVLIRGNQKEIERFMEFLMRSRAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA39</accession>
    <name>A0A218PA39_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Daunorubicin ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_01365</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ABC transporter superfamily. Drug exporter-1 (DrugE1) (TC 3.A.1.105) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854855.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA39"/>
    <dbReference type="GeneID" id="33314878"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01365"/>
    <dbReference type="OrthoDB" id="31298at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043215">
        <property type="term" value="P:daunorubicin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900753">
        <property type="term" value="P:doxorubicin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050763">
        <property type="entry name" value="ABC_transporter_ATP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005894">
        <property type="entry name" value="DrrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025302">
        <property type="entry name" value="DrrA1/2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01188">
        <property type="entry name" value="drrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711:SF18">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13732">
        <property type="entry name" value="DrrA1-3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="4"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="324" mass="37252" checksum="987B656D617EA2B6" modified="2017-09-27" version="1">MWAVEAEDLRKSYPKKIPFPLRKVEWIDAVRGISFRVKRGELFGLLGPNGAGKTTTIKILTTLLEPSGGTARILGLDVRRETREIRRRINLVAEGERTLYWRLSAYENLKYFARIYYVPKNEERERIERLLKLVGLWERRNDLVMNFSRGMKQRLAIAKALINDPEVLFLDEPTLGLDVQSAIFVREFIRRLVDEEGKTVLLTTHYMAEAEQLCDRIAIIDGGKIIALDTPEGLKKLVRDEETVEIHAKDVGPEKIAPSPFKMAVVERNPETGVVRLRAQVDEEELPKLVEWLVRKGVKILSVERKEPTLEDVFIKLTGRGLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA53</accession>
    <name>A0A218PA53_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Haloacid dehalogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_07210</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854913.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA53"/>
    <dbReference type="GeneID" id="33316045"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07210"/>
    <dbReference type="OrthoDB" id="10041at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050155">
        <property type="entry name" value="HAD-like_hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43434">
        <property type="entry name" value="PHOSPHOGLYCOLATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43434:SF1">
        <property type="entry name" value="PHOSPHOGLYCOLATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="220" mass="25784" checksum="418B0E8C0D136095" modified="2017-09-27" version="1">MAVYLFDFDGTLVDSTGAVEKALRIAIEKTIPAVIESDLYDDYYKALFLFIKGKLTYQYLGVIHELVAQGTIHEYYKLMPKYIKDFPFARQLVRTLRKRGRYVISFSGEHTYPGGKVIFMKKTNWYDEFDEVITFKGTKDMLTKFQTLRELHPDEPFVWVDDSPSRFTYILDENTLLVQKASPHKSDVALLFERQNFIKVKSLREILEMDESLIDFENKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA58</accession>
    <name>A0A218PA58_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF86 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_05535</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the HepT RNase toxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA58"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05535"/>
    <dbReference type="OrthoDB" id="25331at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110001">
        <property type="term" value="C:toxin-antitoxin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.580">
        <property type="entry name" value="bsu32300-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008201">
        <property type="entry name" value="HepT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037038">
        <property type="entry name" value="HepT-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01934">
        <property type="entry name" value="HepT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-1277">Toxin-antitoxin system</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="112" mass="12530" checksum="09AB0458A4838B0E" modified="2017-09-27" version="1">MPPSLEEFKSLGLAKDGIYKRLEFAIGLVLEGLAERAKEHGVIAISYGDVVASLREKGLIPEDVAEKATFLAQLREVLIYDYDLVNDEIAFRDMEEYAKFVEDVLAHLEGLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P4X6</accession>
    <name>A0A218P4X6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Radical SAM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_00045</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853098.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P4X6"/>
    <dbReference type="GeneID" id="33314613"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00045"/>
    <dbReference type="OrthoDB" id="15538at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040086">
        <property type="entry name" value="MJ0683-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43432:SF6">
        <property type="entry name" value="RADICAL SAM CORE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43432">
        <property type="entry name" value="SLR0285 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01084">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="4">
        <location>
            <begin position="29"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ05835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="266" mass="30517" checksum="4B42E103A5AE75CD" modified="2017-09-27" version="1">MNLRVIEKKAKSIYTRSKIPGVDWTVNQYVGCAFACKYCYAKFMAKWRDYGEWGSWVEVKTNAPDLARKRVKGSVVMSTVSDPYQPIESGLKLTRRVLQYMDKRNELSILTKSPLVTRDIDIFKLFRSIEVGLTINGFTGREKRLFEPLTPVHEARVSALKELKEAGLRTYVFVSPIIPEITDVSAILGDTRDFADYYFFEVLNLRAAGKEFQALLKEEYPESYGALTDLGKFGEFIRRLKEEIKGLGIKADGIETHQRGWEFVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P4Y9</accession>
    <name>A0A218P4Y9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF371 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_00060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853100.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P4Y9"/>
    <dbReference type="GeneID" id="33314616"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00060"/>
    <dbReference type="OrthoDB" id="9265at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.630">
        <property type="entry name" value="mth639 domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007171">
        <property type="entry name" value="DUF371"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023131">
        <property type="entry name" value="Mth639-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40696:SF1">
        <property type="entry name" value="DUF371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40696">
        <property type="entry name" value="DUF371 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04027">
        <property type="entry name" value="DUF371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ05837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="15768" checksum="B99BC5CB17802372" modified="2017-09-27" version="1">MLREVIHCRGHENVKATHKSTLEFTKEDYLTPRGDCIICIGADKGINDLSEEFKAALKAGKRLLIRIRVSGIVDEVLAEGSPDLILDHDYSMVVRKSTYIDGRTLAVRANKAARDIDRRIVELLKNPGTVAEVELIIDENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P4Z6</accession>
    <name>A0A218P4Z6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Myo-inositol-1-phosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_00140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the myo-inositol 1-phosphate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853116.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P4Z6"/>
    <dbReference type="GeneID" id="33314632"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00140"/>
    <dbReference type="OrthoDB" id="80661at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004512">
        <property type="term" value="F:inositol-3-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052199">
        <property type="entry name" value="MIPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002587">
        <property type="entry name" value="Myo-inos-1-P_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013021">
        <property type="entry name" value="Myo-inos-1-P_Synthase_GAPDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43125">
        <property type="entry name" value="INOSITOL-3-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43125:SF1">
        <property type="entry name" value="INOSITOL-3-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01658">
        <property type="entry name" value="Inos-1-P_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07994">
        <property type="entry name" value="NAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015578">
        <property type="entry name" value="Myoinos-ppht_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myo-inositol-1-phosphate synthase GAPDH-like" evidence="2">
        <location>
            <begin position="212"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ05853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="382" mass="42097" checksum="9857E6B1D252ADF6" modified="2017-09-27" version="1">MVRVVILGQGYVASIFASGLEKIKAGKMEPYGVPLADELPIKIKDIEIVGSYDVDASKVGKDLYEVVRSYDPEAPESLKGITVRKGVHLGSLRNLPIEATGLDDEMTLKEAVDHLVSEWKELKPDVFVNVCTTEAFVPFESREELEKAIEENNKDRLTATQVYVYAAAKYAKEAGGAAFVNAIPTLIANDPAFVELAKESNLVIFGDDGATGATPLTADVLSHLAQRNRYVLDIAQFNIGGNNDFLALTDKERNKSKEFTKSSVVKDLLGYDAPHYIKPTGFLEPLGDKKFIAMHIEYVSFNGAHDELVITGRINDSPALAGLLVDLVRLGKIAVDKKEFGTVYEVNAFYMKNPGPKERGNIPRIIAHEKMRTWAGLKPRWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P513</accession>
    <name>A0A218P513_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FAD synthase</fullName>
            <ecNumber evidence="8">2.7.7.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">FMN adenylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Flavin adenine dinucleotide synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ribL</name>
        <name evidence="10" type="ORF">A3L08_00160</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the transfer of the AMP portion of ATP to flavin mononucleotide (FMN) to produce flavin adenine dinucleotide (FAD) coenzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>FMN + ATP + H(+) = FAD + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17237"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.7.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the archaeal FAD synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.2" evidence="8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853120.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P513"/>
    <dbReference type="GeneID" id="33314636"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00160"/>
    <dbReference type="OrthoDB" id="1912at2157"/>
    <dbReference type="UniPathway" id="UPA00277">
        <property type="reaction ID" value="UER00407"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046444">
        <property type="term" value="P:FMN metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02115">
        <property type="entry name" value="FAD_synth_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050385">
        <property type="entry name" value="Archaeal_FAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024902">
        <property type="entry name" value="FAD_synth_RibL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43793">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43793:SF1">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0274">FAD</keyword>
    <keyword evidence="1 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2 8" id="KW-0288">FMN</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="9">
        <location>
            <begin position="14"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="18"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="23"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ05857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="152" mass="17307" checksum="70F0A8171256A37C" modified="2017-09-27" version="1">MGDENEGKRKVRVLTGGVFDLLHVGHVHFLSQAKALGDELVVIVAHDETVRKRKRREPVNTAEDRAELLKALKMVDEVYIGSPGAIDYELVKRIDPDIIAVGPDQSFNCERLKEDLRKHGINAEVIRIPYLYRSDRAKTSKIIQRIVETYCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P523</accession>
    <name>A0A218P523_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_00235</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853134.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P523"/>
    <dbReference type="GeneID" id="33314651"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00235"/>
    <dbReference type="OrthoDB" id="67699at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46558:SF4">
        <property type="entry name" value="DNA-BIDING PHAGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46558">
        <property type="entry name" value="TRACRIPTIONAL REGULATORY PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="2">
        <location>
            <begin position="5"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ05871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="70" mass="8349" checksum="07DAF5965A4915C3" modified="2017-09-27" version="1">MKNRLRELREERGLTQEELAKALGVTRQTIIAIEKGRYDPSLRLAFKMARFFNVRIEDIFIYEGENDECR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P528</accession>
    <name>A0A218P528_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UPF0200 protein A3L08_00265</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_00265</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPF0200 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853140.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P528"/>
    <dbReference type="GeneID" id="33314657"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00265"/>
    <dbReference type="OrthoDB" id="85381at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01111">
        <property type="entry name" value="UPF0200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022970">
        <property type="entry name" value="NTP_hydrolase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41930:SF1">
        <property type="entry name" value="DEPHOSPHO-COA KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41930">
        <property type="entry name" value="UPF0200 PROTEIN MJ1399"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13207">
        <property type="entry name" value="AAA_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="7"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ05877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="189" mass="21216" checksum="A9877CFDF1763494" modified="2017-09-27" version="1">MIIIVTGMPGSGKSKIVKEFERRGFPSVSMGDIVREETVKRGLELTKENVAKVSIRLRQELGQNAVAKLAVEKVRALLKNSGVAVIDGVRSLDEVGTFRGAFPDERIVIVAVHTPPKQRFERLRARGRHDDPKTWEDFEERDWKELRFGIGGVIAMADYMIINDGSREEYEKKVGEIVELILTSNPGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P549</accession>
    <name>A0A218P549_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sodium:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_00380</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853161.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P549"/>
    <dbReference type="GeneID" id="33314680"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00380"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025383">
        <property type="entry name" value="MrpA_C/MbhD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13244">
        <property type="entry name" value="MbhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="MrpA C-terminal/MbhD" evidence="7">
        <location>
            <begin position="12"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ05898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="76" mass="8036" checksum="385EED7C7DC9E134" modified="2017-09-27" version="1">MLGTILDVIFVAMALLALAVVEEKNLVSSVVKYSLLSLLFVLALFELNAPDVALSAIVVGAVVIGVFIFTIEEVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P590</accession>
    <name>A0A218P590_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P590"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00750"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="441" mass="49580" checksum="9307E09E4E58B54C" modified="2017-09-27" version="1">MARSPVGGVLKLTALLLVLFIVVGNVALRMIPGVKPDGGIKGVLTAPIKVIEYRREHGDNVSVEFGWHSFGGDDERERPPYVRFDGEALWEDVHSPEVRAYVGEIINESIERIQTSGYEVTPQLVRGTAYSLLLQKVHYVHDGRVDLPSYTIEHGGVCSDWALVDYALILAVNERFNVTAEYFFVSSLPPVGVGHAFLAVHYPQAGRWELYDWFATQYLESPYESSRFKVIEIGKNEYLFGWFPAGVAIANQFSSIEEALSVYAMKGGHLQNHYSVTLYRLPEFVRVPQYSTSSFEINTELAMKKIEKIRLRYANYEKQYTLRFEPIGNGGARLLNATVKGDFLRVTFEPLNLSSTVELVAADNSWVVLYDTGGATLAGWKRVTHIGNFPAYKYALLKGKPERLTLLVSLKSDYYEIYPWQVTVRSYMGGNGVRIVLYGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5G1</accession>
    <name>A0A218P5G1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853277.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5G1"/>
    <dbReference type="GeneID" id="33314811"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01035"/>
    <dbReference type="OrthoDB" id="203750at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="526" mass="59672" checksum="42AE8E94E0FA8536" modified="2017-09-27" version="1">MKPGLKVYALGVFLAFILLIGWFFRPWFHGLVMGFYRNPASFYMFAALAAVIVIQVRRPGAAGRKALFPAVLGLIVVISIFASALASPFSNTALYKAYHPSEVTGELNLSTGYIRILPKFTAYRYAIDTIEYARYTLSSGHLTMLNGTPVWGFYIVPDGAWNAIRLKDRGVLFVDMGTTQAKMHRIEEELQVGPAMQVFDNLEWALYKKHYLVDLDLPRALYHNGRLYIVVPYISYSFRVFYTVPRWGGVFIVDEEGNVEDLSPEEALRDERLRDFPLFPEALVREVVEAQNYWKENPFSNIKNLWLHHENQIELIDVSGQGNRQPFLVVANDGRKYWMTAVEPYGKAHGLAAIYLMDARTGEMSQVKFEVPLTGPVKAIDYVKKALPTFDWSQFMAVEPIPVFLEGTLWWRVAIIPRSGSGVAKIAFVNAESKEVKIFENEREVREFLLKGEVVQAEEITGQVKALYSYIKDGNTHWILVVGNRTLYISAADLSEELILKLVSLREGDTVTLKVSEGRVVDIERG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5G5</accession>
    <name>A0A218P5G5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853279.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5G5"/>
    <dbReference type="GeneID" id="33314814"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01050"/>
    <dbReference type="OrthoDB" id="14509at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053199">
        <property type="entry name" value="cDPG_synthetase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42869">
        <property type="entry name" value="SLL0572 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42869:SF1">
        <property type="entry name" value="SLL0572 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobW/HypB/UreG nucleotide-binding" evidence="1">
        <location>
            <begin position="242"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="448" mass="50917" checksum="87542E81A1655E36" modified="2017-09-27" version="1">MAEKRRKRVVILGAAGRDFHNFNVFFRDNPEYEVVAFTATQIPDIEGRVYPPELAGELYPNGIPIWSEDDLEKIIKEHNIDIVVFAYSDVSHEHVMHLASRAHSAGADFWLLGPKSTMIKSTKPVVAVTAVRTGCGKSQTSRKVAQLLQEMGYKVVAIRHPMPYGDLRKQVVQRFATYEDLDRHECTIEEREEYEPYIDRGMVVYAGVDYEKILREAEKEADIILWDGGNNDFPFYEPDLWIVVTDPHRPGHELKYHPGETNFRAADVIIINKIDTANRDDIQKVRESIEKINPNAIVIDGASPLYVDKPELIKGKRVLVVEDGPTLTHGGMKYGAGYVAAKKYGAAEIIDPRPYAVGSIVETYKKYPHLDVILPAMGYGKKQIKELEETINRADADVVVMGTPVDLRRFMNLNKPAVRVRYELEEIGEPKLKDVLKDWVEKCEKLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5G6</accession>
    <name>A0A218P5G6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ATP-dependent protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_01215</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853308.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5G6"/>
    <dbReference type="GeneID" id="33314847"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01215"/>
    <dbReference type="OrthoDB" id="15525at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000206">
        <property type="entry name" value="Archaeal serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="ATP DEPENDENT LON PROTEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="626"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="4">
        <location>
            <begin position="30"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="648" mass="70532" checksum="1A5592F9257FCDDE" modified="2017-09-27" version="1">MKRLAAMAFVLMLLLPVTGFTAAQCPSEGHTVVLKAPAVSRTSNGELTGVATDFVITVAPGTGHVYVETWPLAEVDMQASARLAAQIAGKVLGVDMSKYDVFIHIRADSPIIGGPSAGGTMTVGIIAALEGWAVNPKVMMTGMINPDGTIGPVGGILEKASAAHDVGAELFLIPEGQRIQYVQETQKREIGGIVEINTQTKRVDVVQYAKERWGLTVIEIKDIYDAVYYFTGHRIPRPEAPSYTQIDTSFLKDDALKDYENTTDYYEKTLQKLKDSDVGYGTYATLMEALNEAKDILNQSKKSIDDGLYYTALSKDFQARIIIRHVDWYLDVKTPDDVQELLKGAESQINASESTVSSVNIRGITMLQAIAAAEERVEQAKSLIEEAWKYYYSGDYWNAVGDAAYAYERAKTAEFWAKLGERFAGGEEISRETVKTTARDYIDESNLIVTYIESMYGNVGGDLSGSIQQAERYYEDGKYSAALFTAMEARVQAQVFLDTLGIDNMTVLMDKLKSMKENAKTAIGIAQSNGVTPILAMAYYEFAESYEKSAEENNSLNDLQNAMIFYQYARETAGLFIGGTTTPTVQVPNNTTGIPQIVIPSNAVGPTETPANASVDATGAGKSDELLGVVAVIAFGTFLIGVLVGRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5H2</accession>
    <name>A0A218P5H2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF835 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_01115</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853291.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5H2"/>
    <dbReference type="GeneID" id="33314827"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01115"/>
    <dbReference type="OrthoDB" id="86165at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008553">
        <property type="entry name" value="DUF835"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33531:SF7">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33531">
        <property type="entry name" value="RUBRERYTHRIN SUBFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05763">
        <property type="entry name" value="DUF835"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="DUF835" evidence="2">
        <location>
            <begin position="227"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="352" mass="39586" checksum="E47A13E14A93A6EF" modified="2017-09-27" version="1">MNVILLHTGQTLSFGAKLFAAIYLWYSYRRSFRKSALIFSVAFLTASLQVLGDLIEIETATITMEALFASLLFYGSLKLLDEEGLSFTVSRGYYVSLTPLILTLYMLTAERSSPPNWLATVGVAYVVSGLFILLSGFLLLGLRNLYGNTLKYLGILLIVYGAHEMDYPLLRPVDWFAPFGFSLSAALTLLIAYFFVRFAGQEEFLKLPPERSKPLKEIKPGLTLLSPEEYKKLLPPIKELPLLAFTRNPREAPENWTVYTISQIERERTVSPTNLPKITETVSRYLKTAGNGGIALIDGIEYLNMYNGFEATAKWLSALGDIAYVNNGSVIVVTEREAWNEREWNLLMRLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5H6</accession>
    <name>A0A218P5H6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_01205</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CitM (TC 2.A.11) transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853306.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5H6"/>
    <dbReference type="GeneID" id="33314845"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01205"/>
    <dbReference type="OrthoDB" id="19068at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015105">
        <property type="term" value="F:arsenite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01116">
        <property type="entry name" value="P_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000802">
        <property type="entry name" value="Arsenical_pump_ArsB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051475">
        <property type="entry name" value="Diverse_Ion_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43568">
        <property type="entry name" value="P PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43568:SF1">
        <property type="entry name" value="P PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03600">
        <property type="entry name" value="CitMHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00758">
        <property type="entry name" value="ARSENICPUMP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="95"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="229"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="252"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="280"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="324"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="361"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="402"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Citrate transporter-like" evidence="9">
        <location>
            <begin position="18"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="428" mass="46514" checksum="3AEED542670D9811" modified="2017-09-27" version="1">MEQNIMAAMAVTVFLFTYALIISERVHRTVAALFGAAVVLFIRIVPWEKLPEYLDLDTLFLLIGMMIIVNTAKESGLFEFIAIKTAKFAKGDPMRVLLMFSLVTALVSSVLDNVTTVLLLTPMLLYITKLMDVNPVPFLLSEVFASNIGGTATLIGDPPNIMIGSAAGLSFNDFLLNMGPIAAVDLLLSLGMIYLLYRGTMRVTGDKKDRILSAIEGLREDEAIRDYQLFKKSVTVIIGVVVLFFIHDRLGIEPAVVALFGASTLLLWSRMEPDEILEKVEWTAIFFFVGLFILVGSLVQTGVINDVARWVTGYISGTGEALFIITWFSAIASAIVDNIPLTAAMIPLIKAMGSSMNIYPLWWALSLGACLGGNGTAIGASANVVVIGIASREGVKITFMDFLKVGVIVMFTTVAVGMGLLWVRYIGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5I6</accession>
    <name>A0A218P5I6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pyruvate synthase subunit PorD</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Pyruvate oxidoreductase delta chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Pyruvic-ferredoxin oxidoreductase subunit delta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">porD</name>
        <name evidence="14" type="ORF">A3L08_01325</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterotetramer of one alpha, one beta, one delta and one gamma chain.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853329.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5I6"/>
    <dbReference type="GeneID" id="33314870"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01325"/>
    <dbReference type="OrthoDB" id="23478at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011898">
        <property type="entry name" value="PorD_KorD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053389">
        <property type="entry name" value="Pyruvate_synthase_PorD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040684">
        <property type="entry name" value="PorD_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02179">
        <property type="entry name" value="PorD_KorD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007203">
        <property type="entry name" value="PRK09624.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43724">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43724:SF2">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14697">
        <property type="entry name" value="Fer4_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="13">
        <location>
            <begin position="44"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="13">
        <location>
            <begin position="74"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ06066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="105" mass="12003" checksum="796AC45040EE5D30" modified="2017-09-27" version="1">MAESPFKADIERVQKEYSEKMTPGAIATIPGSSVINKTGSWRVFMPEFNRDKCVRCYLCYVYCPEPAIYLDEENYPVFDYDYCKGCGVCANECPVEAIVMVRETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5J6</accession>
    <name>A0A218P5J6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">2-dehydropantoate 2-reductase</fullName>
            <ecNumber evidence="3 10">1.1.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 10">Ketopantoate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">A3L08_01255</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(R)-pantoate + NAD(+) = 2-dehydropantoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61292"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="9">
            <dbReference type="Rhea" id="RHEA:61294"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(R)-pantoate + NADP(+) = 2-dehydropantoate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16233"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.169"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="8">
            <dbReference type="Rhea" id="RHEA:16235"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ketopantoate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.169" evidence="3 10"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853314.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5J6"/>
    <dbReference type="GeneID" id="33314855"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01255"/>
    <dbReference type="OrthoDB" id="201845at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008677">
        <property type="term" value="F:2-dehydropantoate 2-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003710">
        <property type="entry name" value="ApbA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050838">
        <property type="entry name" value="Ketopantoate_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013332">
        <property type="entry name" value="KPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00745">
        <property type="entry name" value="apbA_panE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005092">
        <property type="entry name" value="PRK06522.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43765:SF2">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43765">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02558">
        <property type="entry name" value="ApbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="4 10" id="KW-0521">NADP</keyword>
    <keyword evidence="6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketopantoate reductase N-terminal" evidence="11">
        <location>
            <begin position="3"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Ketopantoate reductase C-terminal" evidence="12">
        <location>
            <begin position="172"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="304" mass="33116" checksum="86C06B5A76C09F6E" modified="2017-09-27" version="1">MKIYVLGAGSIGSLFGALLARAGNDVTLIGREPQVRAINESGLRVSGVEDFTVHPNASLYAPEEPPDLLILATKSYSTKTALECARKCLGPETWILSVQNGLGNEELALKETPKVIGGVTTNGAMLVEWGHVHWMGKGITVIGKYPTGNEPFVEAVAGIFRKAGLEVSVSENIIGWKWAKAIVNSVINGLGTVLGVKNGVLKDDPYLERISIDIAREGCLVAQQLGVEFEIHPLELLWDTIERTRENYNSTLQDIMRGKRTEVDYIHGKIAEYAQSVGLEAPRNELLWALIKAKENLNKSDGEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5K7</accession>
    <name>A0A218P5K7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF112 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_01280</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853320.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5K7"/>
    <dbReference type="GeneID" id="33314861"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01280"/>
    <dbReference type="OrthoDB" id="96468at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002823">
        <property type="entry name" value="DUF112_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01970">
        <property type="entry name" value="TctA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="252"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="393"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="432"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="DUF112" evidence="2">
        <location>
            <begin position="12"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="452" mass="48146" checksum="162DFE0D3B3946F9" modified="2017-09-27" version="1">MELASLLIQATLLTMFSVFLYVIIGMIPGTDETATIAPITLAFLTAGFDPLLILAWFMGTIVAFKAADAVPTALAAIPGGVMAVPQVPDALVARKYGYSEILLRKGITASVIGTVVAIAITLPLSFYLAPLGELLKNSAGPLNWPGWVYLIVAGTLLLAIMSKAKILALLSIFPFAMLVQGLRGLYGHSAFISIFLAITIGPILYELLTLISPNNHHLRRSDYARIKLVKTRKISLNPLHHLTKAEVTLSSILAGISGILATFMSPVGLTVLFGDLIKESQKDELKGSLMAYAVRDAIKNATYIGGTLIPLIALGVPTGPMSAGPAHPFFAELASFGGLTPRDVLLQRYSTSTIMLVTIYATLFAALLAYTILIKYSKQLTLFVFRKVPAEALYATFLAIVLVLAYNDAGVAGIFGSILVGLISATFVRNGVSIGILFMVLVAAPYLVGLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5N7</accession>
    <name>A0A218P5N7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01455</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853352.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5N7"/>
    <dbReference type="GeneID" id="33314896"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01455"/>
    <dbReference type="OrthoDB" id="11867at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43734">
        <property type="entry name" value="PHYTOENE DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43734:SF1">
        <property type="entry name" value="PHYTOENE DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="420" mass="46807" checksum="F4923EDFB5651880" modified="2017-09-27" version="1">MRAVVIGSGIGGLLTSSFLAKNGYEVTVLEKAPYIGGRFTNLEYRGFGLSTGAFHMLPHGEDGPLAHLLKLLGANVTIVNSDPKGRIFYKGKTFHYRDGWKYLSWRERARAMKLLADVKRNKLPTGEEAEMSGREWIREKIGDNEFVDLFIKSFLGWADSVLDVPAGELAREIKAALKWGGPGLVKGGCNAVTDELARLTEKNGGRILTRKKAVEIDPEEKKVFTADNEEFTYDILISNVGIKETVELMGRDNFDREYLKRVDSLRPSEGIKYNVALKGEPRVGNTVVFTLDTERINGYNEPSSISPELVPEGYTLIMLHHALQSKNVKAEQRKGIEDIYRIFPNLDEEGEILLIQTYLDGNPVNRVASGQTVEDFPIQDVYIVGDAYKLPGGIEVDGIALGVMRTLERLGLGSFSEWYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5P0</accession>
    <name>A0A218P5P0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01490</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853358.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5P0"/>
    <dbReference type="GeneID" id="33314903"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01490"/>
    <dbReference type="OrthoDB" id="92175at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="71" mass="7909" checksum="1909CFF4AB18269C" modified="2017-09-27" version="1">MFGRRKDIVYKTLATKKRAVALQKLSAELETPASAVFKTVKELESDGLVEVFYGQDKAAIMVRAKTIGDYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5P9</accession>
    <name>A0A218P5P9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF3213 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01540</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853367.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5P9"/>
    <dbReference type="GeneID" id="33314913"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01540"/>
    <dbReference type="OrthoDB" id="86081at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1750">
        <property type="entry name" value="Uncharacterised protein PF11491, DUF3213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021583">
        <property type="entry name" value="DUF3213"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11491">
        <property type="entry name" value="DUF3213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="103" mass="12202" checksum="A57CD5F975373A8D" modified="2017-09-27" version="1">MTTERVFNKKLTRLNFKFGNIDWEKATIKQYELEKELGVWRIFLNGYAKNGFVIFDEELIPKEKLLEALKELEPEITAIKRLTVEELIESSYSWNNILGKMES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5T5</accession>
    <name>A0A218P5T5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cation:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853402.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5T5"/>
    <dbReference type="GeneID" id="33314954"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01745"/>
    <dbReference type="OrthoDB" id="19138at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005133">
        <property type="entry name" value="PhaG_MnhG_YufB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01300">
        <property type="entry name" value="CPA3_mnhG_phaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009312">
        <property type="entry name" value="PRK12672.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34703">
        <property type="entry name" value="ANTIPORTER SUBUNIT MNHG2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34703:SF1">
        <property type="entry name" value="ANTIPORTER SUBUNIT MNHG2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03334">
        <property type="entry name" value="PhaG_MnhG_YufB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="123" mass="13258" checksum="403033C5C4AA24FA" modified="2017-09-27" version="1">MSWVDYLIYAFLAISLTFNALGSIALHRFPDVYTRLHGATKCTTFGTIFAVLAVVTHALYQLHVTGDSKYLQMALHSVVALIALLLTNPVGAHAIAKAAHLSGYKPAKAVVDAYEEKLGGGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5U3</accession>
    <name>A0A218P5U3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Hydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_01780</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I 20 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853408.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5U3"/>
    <dbReference type="GeneID" id="33314961"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01780"/>
    <dbReference type="OrthoDB" id="5740at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052375">
        <property type="entry name" value="Complex_I_20kDa-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005012">
        <property type="entry name" value="PRK06411.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42989:SF1">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42989">
        <property type="entry name" value="HYDROGENASE-4 COMPONENT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56770">
        <property type="entry name" value="HydA/Nqo6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase-like 20kDa subunit" evidence="7">
        <location>
            <begin position="44"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="173" mass="19049" checksum="0D905324A44228DE" modified="2017-09-27" version="1">MSIKVPVEKKENQSERERLEKRIAQLCRFLGRSPWVFHINSGSCNGCDIEIIAALTPRYDAERFGVKLVGSPRHADILLVTGPVTNQSLERVKLVYEQTPEPKMVIAVGACPTGGSVFYESPFTNAPLDRVIPVDVYVPGCPPRPEAILQGVVLALEKLAKILKGEVPEVSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5U8</accession>
    <name>A0A218P5U8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PadR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_01880</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853427.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5U8"/>
    <dbReference type="GeneID" id="33314981"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01880"/>
    <dbReference type="OrthoDB" id="56053at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43252:SF2">
        <property type="entry name" value="TRANSCRIPTION REGULATOR, PADR-LIKE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43252">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR YQJI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator PadR N-terminal" evidence="2">
        <location>
            <begin position="19"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="134"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="165" mass="19271" checksum="FEB8155B74AFC4BA" modified="2017-09-27" version="1">MQDSNVVRNLYTVPMKNMILLIIGLKGEAHGYEILKEIEKMTFGNWKPSHGNLYTMLNKLAEEGLIEPREEYRGKRRIVKYALTENGWLYLREANELALKSLYLAVQYHERLREKLRELGYGKEIAATAIDSYIELLDGIIDILEEKKRELKELKREKELRGTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5X2</accession>
    <name>A0A218P5X2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF835 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_01970</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853445.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5X2"/>
    <dbReference type="GeneID" id="33314999"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01970"/>
    <dbReference type="OrthoDB" id="86083at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008553">
        <property type="entry name" value="DUF835"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05763">
        <property type="entry name" value="DUF835"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="DUF835" evidence="2">
        <location>
            <begin position="126"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="256" mass="29224" checksum="F8BE777E3B218CAC" modified="2017-09-27" version="1">MELTVPWYILVYDVVLLVGMGYIWWFFLKRWNRYVAELRPFIRSAAVFIGFAFVGRLLDLITDFYPLPYQDAAFAVLYGVSILGVIYAMINYVLLLERSYIPPKTVEFKPNPSGLMGAYVLLGGREKLVEIVKIIREHGMPALIFTRSPHFYAGLGELVTTVWVTQATDAGVPPTKLHVIQETAIQFAKEVKGSVIVIDCVEYLLLYNDFKTVFKFLTNLRDHIVLKYGSGLILFIDNAVLSEREKALLLKEFEPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P634</accession>
    <name>A0A218P634_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DHH family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853513.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P634"/>
    <dbReference type="GeneID" id="33315075"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02350"/>
    <dbReference type="OrthoDB" id="86112at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDH" evidence="1">
        <location>
            <begin position="3"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="311" mass="35424" checksum="AD7C5EA75E509C63" modified="2017-09-27" version="1">MHLIVHHWDTDGVTSAALLVRALGLKEFTNMTAPIGEFRFDGRIWDAIERAERLYVLDFNVPDEVEKVKVPTLFIDHHTQPKIKNPLVEQVNPSLEGKYYPSCSLVVSEYFGIWNAWSALGVVGDIGEKAFELETVNELLRKANLSRTEALRLVELIDSNYIAMEREAVEEAVTVLLESEIKDLLEYEPWIKKAKAIRDAIENALSSVEEKNGFALVEFESPYNVISKVARKLVWEMNYRGAVVVNRNFHGKAQVYFRISPEEAERIDMGKVITRLRGLGTNAGGKREVLGCVCERDKIDDVLKIIEEYLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P640</accession>
    <name>A0A218P640_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Nucleotide pyrophosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_02345</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853512.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P640"/>
    <dbReference type="GeneID" id="33315074"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02345"/>
    <dbReference type="OrthoDB" id="33550at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="476" mass="55247" checksum="8A1ADA0280041006" modified="2017-09-27" version="1">MEFGKKVREGMENTKRVFVIGLDSAPPELLFNRFIDDMPNVKRLLEKSVYGPMQTGIPAITIPMWMVMVTGKTPGELGLYGFRHRTGYSYTDYWIAHSRKVKEPTLWDYLGERGKESIIVGVPPTYPPKPIKGHLVSCFITPDASVDYTYPKELKGEIERLVGEYIFDVPFRKEAKDEVKEGIWEMTEKRFEVIRYLLQEKDWDYFHFVEIGLDRLHHAFWRYFDENHHLYPGKGNKYENVIPDYYKLLDKEIGETLKLIDLDETAVFIVSDHGIKAMHGNFAVNQWLAEEGLLKVRNPEVLHDGKVKRFEQIEIDWKETTAWGWGGYYSRVFLNVLGREKEGKIPLSKFEKVRDEVAEQIKSIRGPNGEKWDTKVFYPEEIYPEAKGSKPDIMVYFDNLNWRAAGTVGHPTNYLPENDTGPDDANHSEFGVFSMYLPGFDESRATQLTIYDFAPTILRLFGIEEPIKSMHGRSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P643</accession>
    <name>A0A218P643_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Sulfotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_02325</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853508.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P643"/>
    <dbReference type="GeneID" id="33315070"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02325"/>
    <dbReference type="OrthoDB" id="96665at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="322" mass="38269" checksum="3DE8A01422F2ACDB" modified="2017-09-27" version="1">MRPLLLKTLAKFNMLVDIAASRIHNYSPKDTIAVFGSRRSGSTWLMEILESLPEYRSVFEPFHPRYYPEFGRIGFPYDPYVPLPGEYNRIREYLERSLSGKVYIQFPYRMGVIKRLKGSKLVVKFVRGNTILPWAARTFKIRGYYFIIRHPCATIASQLTTGYHSRITVEQLLNEVNKVPELSENKKLIMHLMGINSEIKRLAAIWAFENYIPLASEKPYPWYTVVYERLIREPEEEFRRVFRYIGEEVPEEAWKKMRKPSMVTRKSYGREYIGTPKQLLKWKDQLSERQVKDILEVVSWFGLDFYDERPEPDYDALARWRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P661</accession>
    <name>A0A218P661_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853544.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P661"/>
    <dbReference type="GeneID" id="33315110"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02520"/>
    <dbReference type="OrthoDB" id="95481at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="175" mass="20116" checksum="20C3C81EE36B4CE7" modified="2017-09-27" version="1">MKISYETSFRLKVLAIAVLFGLIIFYLVYYPIISHNPVPYGVASPRGQILLMQNITLGDFSWNNAVDLYNNLVLKGDEDYSDYVVVRLTTPGWCMDAVVWDGTKYTKRASCVREVTISRYTFRIPPGSYWYLDGSYHLILYKPEGTPENYELVNFTVTYGPKSDWGAFKATYPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P662</accession>
    <name>A0A218P662_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">YkgJ family cysteine cluster protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_02525</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853545.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P662"/>
    <dbReference type="GeneID" id="33315111"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02525"/>
    <dbReference type="OrthoDB" id="36424at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005358">
        <property type="entry name" value="Puta_zinc/iron-chelating_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35866">
        <property type="entry name" value="PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35866:SF2">
        <property type="entry name" value="YKGJ FAMILY CYSTEINE CLUSTER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03692">
        <property type="entry name" value="CxxCxxCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="175" mass="20471" checksum="9A33F89E5DA8E3BB" modified="2017-09-27" version="1">MRFKPKPFTEPVPFKCLYCLDCCRGRHVYLTLKDIERIARKGHDPQDFVTFSVEGNQIRFVLAVREWDLGCVFHDPETGKCKIHDANPIICRIYPFMVSRKPLGVEGEKPFEYKGQTLWLYYDESCPGINAEEPETAIDPEEIAELGLEFEREFERTDMEGFVELIERLEITETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P673</accession>
    <name>A0A218P673_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">A-type ATP synthase subunit D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">atpD</name>
        <name evidence="12" type="ORF">A3L08_02610</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the V-ATPase D subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853561.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P673"/>
    <dbReference type="GeneID" id="33315128"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02610"/>
    <dbReference type="OrthoDB" id="117390at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.3240:FF:000007">
        <property type="entry name" value="V-type ATP synthase subunit D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00271">
        <property type="entry name" value="ATP_synth_D_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002699">
        <property type="entry name" value="V_ATPase_D"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001545">
        <property type="entry name" value="PRK00373.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00309">
        <property type="entry name" value="V_ATPase_subD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11671">
        <property type="entry name" value="V-TYPE ATP SYNTHASE SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01813">
        <property type="entry name" value="ATP-synt_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="140"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="217" mass="24992" checksum="E8024E5105540342" modified="2017-09-27" version="1">MAELLNVKPTRMELLNLKRRITLAKKGHKLLKDKQDALVMEFFTIYDEALSLRRELGMKMEEAFNALQAAEIDVGTLRLREISLSVKPNREVEVKKRNVMGVPVPLIEAESFRRSAEERGYAFVSSSAKVDLAAEKFEEVLDLAVRLAEVEETLKRLAKEIEVTKRRVNALEYIIIPRMEATVKFIKGRLDEMERENFFRLKRVKALIEARSQAEGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P697</accession>
    <name>A0A218P697_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Radical SAM family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_02640</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853567.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P697"/>
    <dbReference type="GeneID" id="33315134"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02640"/>
    <dbReference type="OrthoDB" id="53113at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43787:SF3">
        <property type="entry name" value="ARYLSULFATASE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43787">
        <property type="entry name" value="FEMO COFACTOR BIOSYNTHESIS PROTEIN NIFB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="52"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="302" mass="34424" checksum="8766C5BF519D0143" modified="2017-09-27" version="1">MKRMSWEEFARSMGVEPQILENKEARLLKQFVMDLKFPTHCQGCQGLDLNNPNPVHHPSYELTPACNHDCIFCYSNVAVKLGKAPKPGYYGWEDPYAITVSQYGEPLLSPRIVEVNKMLRERFPNARLDLQTNGSLLTEELWKKLDFDLVMISLDAASREKHLKITNADTFDAVVNALKIVGNDKSVRSVVRTIFMPGINDEDIPKIAELAASLGIDEMMLQPLTIHELNVERLKKAGLDFDRAESVREFLKAAMEAKKHIDVRISGCQLAIYRTMDPLTLFSARRIARDVVPIVKRERKER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6D5</accession>
    <name>A0A218P6D5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycoside hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_02915</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853618.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6D5"/>
    <dbReference type="GeneID" id="33315189"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02915"/>
    <dbReference type="OrthoDB" id="84549at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054046">
        <property type="entry name" value="PSMA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054047">
        <property type="entry name" value="PSMA-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22160">
        <property type="entry name" value="PSMA-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22159">
        <property type="entry name" value="PSMA-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Maltose-forming alpha-amylase-like N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Maltose-forming alpha-amylase-like C-terminal" evidence="2">
        <location>
            <begin position="322"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="578" mass="66637" checksum="83C2D5AC1A81A961" modified="2017-09-27" version="1">MFMKYAHHFHAYQPGDIVYVHDGDGSKPIEYEERKSPVAVKIRGEEVRGENWTRAMLYSYEHISDTLSRMKGISVDIEPFTFLMLLRYHRKAFEETVGLLQGFDAVPTTPFHPIVPHLDEFEQRILAKVSFDFYTPFTGEKDVIGYWLPEAVITRKSASIVESSTDKRLVFLLDERQLLYDFPQAKHSCNRYGNSFVFGREWRISDAFAFNTLDVPGLVSATLAYRDDYKENLGVPYLLFTASDLESLLGNPAQLDRFTAWMGGLEENGVERVSAMEFVRKKLSGEFRRLDGECSFEMGVKDYSAWSDYFDLSLDGKTSDSRWLGYRRADGKVFAREVKGRKVSQVWKVAFTRLFEELNRFVRLGVLKGLEDLGVGSQNAEEFLVRYARVFFRDYYDYFGVETSLGYVLEPVNGDREALKLGRAYYLMLLANHSCPRFWENLDTRVSFGNVSAIAKAIIELMRYFDGSETKSIFIEAYLRLLNFEGLHHLWDLGSMEGLEGWETTENAWSEALKPEVPNSGYNVVTRAALYVGKRDLRGELRSLIEPYNLEWAVADTGHIPGEVHGHWENSEWCEHRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6K9</accession>
    <name>A0A218P6K9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycosyl transferase family 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_03105</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853652.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6K9"/>
    <dbReference type="GeneID" id="33315227"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03105"/>
    <dbReference type="OrthoDB" id="132546at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12526">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12526:SF629">
        <property type="entry name" value="TEICHURONIC ACID BIOSYNTHESIS GLYCOSYLTRANSFERASE TUAH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="3">
        <location>
            <begin position="195"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="379" mass="44110" checksum="BDE2D4A8A8F8B7C4" modified="2017-09-27" version="1">MKILVISLPDLQKIPIQRYHHLIKEISKKHDVTVLSVNAWWLQEIEESKRFLQNVDYHYITKRRIHPIIQEALIVHHISKIKRSNFDLIINFNTLIATLLVKSFIPTIPLLFDIADELISGILDSPKFPKFMKNALSLKILRLLLSFNVSKATIVTIVSENLKRKYNLPDARLIYNGVRKDLMYSFYTPRRQREADMTLGFVGVVREWVDLHPVFNAISKLKFEYKINFLIIGDGPLIGYYKKLAQKLNIEKNVQFLGFIPHSKIPRYLQSIDIGLIPFNQSEIAIFAFPIKLLEYFSLKKPVISSRLPAIENNFGSTVFYAETNDEYLQILKDVMNNKKLLKKKGVRGYKITKNQYTWEHIGKTLNLVLSKIVQGVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6N4</accession>
    <name>A0A218P6N4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">S26 family signal peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_03400</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853701.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6N4"/>
    <dbReference type="GeneID" id="33315286"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03400"/>
    <dbReference type="OrthoDB" id="50404at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.109.10">
        <property type="entry name" value="Umud Fragment, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001733">
        <property type="entry name" value="Peptidase_S26B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02228">
        <property type="entry name" value="sigpep_I_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806:SF6">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00728">
        <property type="entry name" value="SIGNALPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="355" mass="39454" checksum="415B41E54027FF6E" modified="2017-09-27" version="1">MKKLIENIIVFIVIMFLITSVMGFVLDRPVLISYAYSESMTPTINKGDLFFINPLSKGGEVGDIIVFHRRDGWTVHRIFAVTDEGYITKGDNNVATDQQDGAYPPVNREDIIGKVMAVGNHPLVIRGGGALIESARSKLTNIYAVAVILLLGAFLTFSGGNNRRGHGRKKRFLKLSMRTVYAVVSVGIVAGFIFVTVASWGTLAFTYSSTLASGQMEGWYLPGSTFEKNLSIENRAIYPFHYFIGTKSVRVTILNEKQFKIDGKGSHEVLMKVSVPEDTKIYREEIEVRSYPAILPGRLVAAMYKINPYLPLIPYTLELAAILLALYYMADIGQGEVIRIRIRRRSLLSKIAGDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6N8</accession>
    <name>A0A218P6N8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoribosylformylglycinamidine synthase subunit PurQ</fullName>
            <shortName evidence="8">FGAM synthase</shortName>
            <ecNumber evidence="8">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Formylglycinamide ribonucleotide amidotransferase subunit I</fullName>
            <shortName evidence="8">FGAR amidotransferase I</shortName>
            <shortName evidence="8">FGAR-AT I</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Glutaminase PurQ</fullName>
            <ecNumber evidence="8">3.5.1.2</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Phosphoribosylformylglycinamidine synthase subunit I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">purQ</name>
        <name evidence="9" type="ORF">A3L08_03270</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="8"/>
    <dbReference type="EC" id="3.5.1.2" evidence="8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853678.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6N8"/>
    <dbReference type="GeneID" id="33315260"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03270"/>
    <dbReference type="OrthoDB" id="6486at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01740">
        <property type="entry name" value="GATase1_FGAR_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00421">
        <property type="entry name" value="PurQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010075">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurQ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01737">
        <property type="entry name" value="FGAM_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002957">
        <property type="entry name" value="PRK03619.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47552">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47552:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13507">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001586">
        <property type="entry name" value="FGAM_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01211">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="223" mass="24672" checksum="B4C5F5C0F5621B9A" modified="2017-09-27" version="1">MVRFAVVVFPGTNCDFETERAIRKAGAEAERVWYKRSLKDFDGVVLPGGFSYADYLRAGAIAARQEIMGEVKEFAREGRPVLGICNGFQILTEAGLLPGALRPNKISRFLCRWVHLRVADNETPFTSLYEPGEVIRMPIAHAEGNYYVDNPSKVRIVFQYSDEEGNVTEAANPNGSVLNIAAVANGRGNVLGTMPHPERASDSFLGSEDGLKVFKSMVEWARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6S9</accession>
    <name>A0A218P6S9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Antitoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03730</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6S9"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03730"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="61" mass="7364" checksum="625D3478B80336D2" modified="2017-09-27" version="1">MSEVIERLERIEKLLIALNSKVDNFLGYESLTKKEREELRKMREEVKRGDFVKFEELFGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6T5</accession>
    <name>A0A218P6T5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03710</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853752.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6T5"/>
    <dbReference type="GeneID" id="33315347"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03710"/>
    <dbReference type="OrthoDB" id="101258at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="90" mass="10747" checksum="CC779D4CB18F9AF4" modified="2017-09-27" version="1">MEEVNRLVFNFPLFKDYEEKERFFKVIGLLVSHQITLEKAAELLDMKLEELSFLLDKLGVEYSLLDEEEAELELSKLKEIREELKREGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6T7</accession>
    <name>A0A218P6T7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S58 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853745.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6T7"/>
    <dbReference type="GeneID" id="33315339"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03670"/>
    <dbReference type="OrthoDB" id="85110at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02253">
        <property type="entry name" value="DmpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.70.12">
        <property type="entry name" value="L-amino peptidase D-ALA esterase/amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016117">
        <property type="entry name" value="ArgJ-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005321">
        <property type="entry name" value="Peptidase_S58_DmpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36512:SF3">
        <property type="entry name" value="BLR5678 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36512">
        <property type="entry name" value="D-AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03576">
        <property type="entry name" value="Peptidase_S58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56266">
        <property type="entry name" value="DmpA/ArgJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="347" mass="37541" checksum="40B18610D858A8F3" modified="2017-09-27" version="1">MKAPELGIKIGIHEHGKRNSIADLGVRVGHKTLIEGENIRTGVTVLLPPVKNPYRERLFASTFVMNGFSKPIGFVQVNELGYIETPIALTNTLSVYTVASAMVRHMIELNPDLKSVSPVVMECNDSYLNDIRRMAVREEHYFEAVKRAKLDFEEGSVGAGTGMSAFEFKGGVGSSSRVVEIGGEEYTVASLVLANFGRREDLTIAGVPVGLCLKDYPGRGTSGKGSISMVVATDAPLTARQLKRLAKRAVVGLARTGGYAYHGSGDVVLAFSTAQTVPMGKEPELVSFLPDNSLSRLFRATAEATEEAIINALLQAKTVEGNGHVRYALPVEKVLEILERHGMIERP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6U6</accession>
    <name>A0A218P6U6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Replication protein RepA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853738.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6U6"/>
    <dbReference type="GeneID" id="33315329"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03620"/>
    <dbReference type="OrthoDB" id="85901at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="125" mass="14451" checksum="C44D47CA0B813852" modified="2017-09-27" version="1">MEEIKFRRRKPAVERKIGEIREDDTRVSLIGKAFKVDKMDYTFWLDDGTGVILVESEENVLPENGQIVRVIGRVIRNEEGTHIYGEVIQDFSNADLDALEEVRELERKVLPKVESIIEFFGGEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P700</accession>
    <name>A0A218P700_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl10e</name>
        <name evidence="5" type="ORF">A3L08_04030</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853812.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P700"/>
    <dbReference type="GeneID" id="33315410"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04030"/>
    <dbReference type="OrthoDB" id="30538at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1170.10:FF:000008">
        <property type="entry name" value="50S ribosomal protein L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="entry name" value="Ribosomal protein L10e/L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00448">
        <property type="entry name" value="Ribosomal_uL16_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047873">
        <property type="entry name" value="Ribosomal_uL16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022981">
        <property type="entry name" value="Ribosomal_uL16_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018255">
        <property type="entry name" value="Ribosomal_uL16_CS_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_uL16_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001197">
        <property type="entry name" value="Ribosomal_uL16_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_uL16_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003237">
        <property type="entry name" value="PRK04199.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003239">
        <property type="entry name" value="PRK04199.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11726">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005590">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="Ribosomal protein L16p/L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01257">
        <property type="entry name" value="RIBOSOMAL_L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="182" mass="21050" checksum="CC56F8EA0F3791F6" modified="2017-09-27" version="1">MGLRPAKIDRDVDKPAYTRREYIRGAPGPKITIFDMGNLSAEFEYEVSLHAEQAMQIRQNALEAIRIQVNRYLQKNVGRSNYHFKIRVFPFQVLRENPMATGRKADRYGNGMRRPFGKPIGLAARVKKDQKILTVWVNEAHLKFALGAMHRAKMKLPYSAYYRIYDKEGNDVTTKVLSTMKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P723</accession>
    <name>A0A218P723_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein uL30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rpl30p</name>
        <name evidence="5" type="synonym">rpl30</name>
        <name evidence="7" type="ORF">A3L08_04155</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2 5">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853835.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P723"/>
    <dbReference type="GeneID" id="33315435"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04155"/>
    <dbReference type="OrthoDB" id="6379at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.15.30:FF:000002">
        <property type="entry name" value="50S ribosomal protein L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.15.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01371_A">
        <property type="entry name" value="Ribosomal_uL30_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_uL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005997">
        <property type="entry name" value="Ribosomal_uL30_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018038">
        <property type="entry name" value="Ribosomal_uL30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_uL30_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004711">
        <property type="entry name" value="PRK06049.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01309">
        <property type="entry name" value="uL30_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00634">
        <property type="entry name" value="RIBOSOMAL_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="6">
        <location>
            <begin position="5"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="155" mass="17639" checksum="8B03197EE58E3B05" modified="2017-09-27" version="1">MAKLALIRLRSGIRAKGEVRDTLAMLRLHRINHLVLVDDTPSYKGMVQKVKDYITWGEINKETLAELIRKRGRLIGNKPVTDEYVKEKLGMTIDEFAEKVVSGEMKLTDLPNIKPVFRLHPPRGGLKGSKKRSFKEGGALGYRGEKINELIERML</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P746</accession>
    <name>A0A218P746_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Adenine phosphoribosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_04320</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853860.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P746"/>
    <dbReference type="GeneID" id="33315468"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04320"/>
    <dbReference type="OrthoDB" id="31493at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050118">
        <property type="entry name" value="Pur/Pyrimidine_PRTase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006328">
        <property type="entry name" value="PRK08558.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43864:SF3">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE, FUSED TO N-TERMINAL DNA-BINDING DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43864">
        <property type="entry name" value="HYPOXANTHINE/GUANINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="2">
        <location>
            <begin position="17"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="1">
        <location>
            <begin position="107"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="238" mass="26966" checksum="C8D30509BF038454" modified="2017-09-27" version="1">MSQLKSVQEKLRLVRVLRLLKKTYTYEELSKITGLPITVLNRYVRGKVLPSAERTKELLNLLLPYINIEEEVRKRIKFDEYGFFDNMPVLSDTALMSLIAEDVAGRYMDMNVDKVLTAATDGIAFGVHVARELNVDVVYAKKKKEVGVEKFYEVSYVPSASGSVTTLYLPQWALRKGENVLIVDDVIRSGETQRALLEMCRQAGAKPVGMFFLISVGDVIDHLKEEYSIPVESLIKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P756</accession>
    <name>A0A218P756_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">Adenylate kinase</fullName>
            <shortName evidence="12">AK</shortName>
            <ecNumber evidence="4 12">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">adkA</name>
        <name evidence="13" type="ORF">A3L08_04170</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the archaeal adenylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="4 12"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853838.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P756"/>
    <dbReference type="GeneID" id="33315438"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04170"/>
    <dbReference type="OrthoDB" id="26198at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00234">
        <property type="entry name" value="Adenylate_kinase_AdkA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023477">
        <property type="entry name" value="Adenylate_kinase_AdkA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003122">
        <property type="entry name" value="PRK04040.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13207">
        <property type="entry name" value="AAA_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 12 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="9"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="196" mass="22267" checksum="F476AC32DBB20D26" modified="2017-09-27" version="1">MPFVVMITGIPGVGKSTITRLALKKSRAKFRLVNFGDLMFDEALKSGLVKHRDEMRKLNPNVQKELQLKAARRIVEISQSEPVLIDTHATIRTPVGYLLGFPREVIEIINPNFIVIIEANPSEILGRRLRDLKRDRDVETEEQIQRHQDLNRAAAVSYAMHSNALIKIIENHEDKGLEEAVHELVEVLDLAVGEYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P762</accession>
    <name>A0A218P762_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_04410</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853874.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P762"/>
    <dbReference type="GeneID" id="33315486"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04410"/>
    <dbReference type="OrthoDB" id="27015at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030665">
        <property type="entry name" value="KaiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010624">
        <property type="entry name" value="KaiC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637:SF1">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039117">
        <property type="entry name" value="KaiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01874">
        <property type="entry name" value="DNAREPAIRADA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51146">
        <property type="entry name" value="KAIC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="KaiC" evidence="10">
        <location>
            <begin position="1"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="RecA family profile 1" evidence="9">
        <location>
            <begin position="234"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="KaiC" evidence="10">
        <location>
            <begin position="236"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="453" mass="51189" checksum="22FCC296B5414094" modified="2017-09-27" version="1">MRKMGIDFFDEQVVEGGFPEGALILVAGEPGSGKTIFSSTWLYNGAKKFGEKGVYISFAETKTDFFEEMKQLGMDFGELEQKGLFKFIDLVTVSSETIEKEIELIMGEILTFQPQRVVLDSISVFGQLMGQAKLRAFLHTTLGRFMKATGATVLLIAEKPMGQEKVGYGIEEFVVDGVIVLKSKSLGESLIRLMEVRKMRRRSIKKPQFEYTISSHGIEFLDIPELRRAEIEPTWEKISTGIKKLDEIVGGGLYRGSSVLLVGMTGTGKTTFALHFAVNNALEGRKALYLAFEEPMDQLLRAAKNYGLPIEKVLNGNLNILNIIPEAHTPVQTFIRIKRAIEEHKPEVLVVDSLTALKQHMNEKELTKMLRYLTLLTKEYRTTIYFTLNDETDFRVVPMTGASTLTDVIIGLRYELVNDRIERRLAVVKARGSNHSRKIYRYEITDEGVVIYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P766</accession>
    <name>A0A218P766_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Serralysin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_04425</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853877.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P766"/>
    <dbReference type="GeneID" id="33315489"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04425"/>
    <dbReference type="OrthoDB" id="26738at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43281">
        <property type="entry name" value="FARNESYL DIPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43281:SF1">
        <property type="entry name" value="FARNESYL DIPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01017">
        <property type="entry name" value="Polyprenyl_Transferase_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="343" mass="38521" checksum="8F1385A039717119" modified="2017-09-27" version="1">MGKYDELFMRIKEKAKDVDRVILELVPEKEPKSLYEASRHYPLAGGKRVRPFVVLRATEAVGGDPEKAIYPAASVEFIHNYSLVHDDIMDMDELRRGRPTVHKLWGVNMAILAGDLLFSKAFEAIAKAEVPPEKKARILDVLVRTSNMLCEGQALDIEFETREEVGVDEYLRMISGKTGALFQGSAEIGAIVGTDNEEYIQALSKWGMNVGIAFQIWDDVLDLIADEEKLGKPVGSDIRKGKKTLIVSHFFDHASEEDKTEFLKVFGKYAGDAKGDALIHDEKVKEEVAKAIELLKKYGSIDYAAQYAKNLIKEANEALKVLPESEARRDLELLAEFLVEREF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P788</accession>
    <name>A0A218P788_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable tRNA pseudouridine synthase D</fullName>
            <ecNumber evidence="4">5.4.99.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">tRNA pseudouridine(13) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA pseudouridylate synthase D</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA-uridine isomerase D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">truD</name>
        <name evidence="6" type="ORF">A3L08_04580</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Could be responsible for synthesis of pseudouridine from uracil-13 in transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>uridine(13) in tRNA = pseudouridine(13) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42540"/>
            <dbReference type="Rhea" id="RHEA-COMP:10105"/>
            <dbReference type="Rhea" id="RHEA-COMP:10106"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the pseudouridine synthase TruD family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.27" evidence="4"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853903.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P788"/>
    <dbReference type="GeneID" id="33315520"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04580"/>
    <dbReference type="OrthoDB" id="1798at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160150">
        <property type="term" value="F:tRNA pseudouridine(13) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3160:FF:000001">
        <property type="entry name" value="Probable tRNA pseudouridine synthase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1510.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.20">
        <property type="entry name" value="TruD, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01082">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001656">
        <property type="entry name" value="PsdUridine_synth_TruD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020119">
        <property type="entry name" value="PsdUridine_synth_TruD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011760">
        <property type="entry name" value="PsdUridine_synth_TruD_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042214">
        <property type="entry name" value="TruD_catalytic"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00094">
        <property type="entry name" value="tRNA_TruD_broad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326:SF21">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE PUS7L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01142">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037016">
        <property type="entry name" value="Pseudouridin_synth_euk_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50984">
        <property type="entry name" value="TRUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01268">
        <property type="entry name" value="UPF0024"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TRUD" evidence="5">
        <location>
            <begin position="158"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="416" mass="48202" checksum="B8371DD176F25E36" modified="2017-09-27" version="1">MDYREFFSQFHYLSEKPGIGGRIKVFPEDFVVIEDPLPSIFEGKKYAIFLLKKRNWDTMAAVKEIAKRAGIRHRDIGFAGTKDRHAVTYQYISVPAQAKEKVEGVQIRDIELRFVSYGRFIKLGHLIGNRFRIIVRDVGEEAFERTREIVRELREKGGFPNYFGYQRFGERRVTNHLIGKLLLQGDFEGAARLFLGAHEGGMEGDEARKNFWETGDVDRALEEFPNFLRYERAMLHRYKETGSWKRAFLSLPLPIMRIFIHAYQSYLFNLYISRRIEEGLPLNEALVGDIVVQVKGGIPYRDRTYRVTETNKDFVNEKIRRNQAMVSGPLFGFSMRTAKGLPGRLEEGILKEEGISLETFKNLPKPMAEPGGRRELLIRPLGMIYGYVPDTGMCFRFFLPKGVYATSVLREIMKDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7A0</accession>
    <name>A0A218P7A0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">D-glutamate cyclase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_04610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853909.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7A0"/>
    <dbReference type="GeneID" id="33315526"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04610"/>
    <dbReference type="OrthoDB" id="86109at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047820">
        <property type="term" value="F:D-glutamate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006536">
        <property type="term" value="P:glutamate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1640.20">
        <property type="entry name" value="TON_0340"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025504">
        <property type="entry name" value="GLUCM_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32022">
        <property type="entry name" value="D-GLUTAMATE CYCLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32022:SF10">
        <property type="entry name" value="D-GLUTAMATE CYCLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14336">
        <property type="entry name" value="GLUCM-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-glutamate cyclase-like C-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="269" mass="28882" checksum="A43C9309CFB78CE4" modified="2017-09-27" version="1">MIAHLITTDVGNRGVLGVYLDYRRENPNFLHNSAEMFLDNYSRVLIVTGFPIPPDMTPETDGPPGALALAKAVQSLGGRAEVLTYPEVGKALERFGVKLAEKPELGNYSLVIAVETPGRAADGKYYSMSGLEITRETFDWAVIEAQKLGIPTIGIGDGGNEAGMGKIRKLVERHVPHGERIASVVEVEELVLSAVSNWGAYGLLAEASVELGRNLLADWDEEKAVKAMMNAGLIDGVSKKLAPTVDGIPLEVHRGIVELLKALVNEALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7A1</accession>
    <name>A0A218P7A1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_04620</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853911.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7A1"/>
    <dbReference type="GeneID" id="33315528"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04620"/>
    <dbReference type="OrthoDB" id="85717at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="251" mass="29343" checksum="D476A77068F9EFD6" modified="2017-09-27" version="1">MQSFTHLFVGIGNAGARIVNNIHADGTVRVTVNPAYLLMPRAQAHEKKLRDFFSRLPENTFLWLIFEDKEANHLVREIILDSVPSDTINLAYVLTPKRELVTEKKPPWAEQFETVFYDSLWEFFRGPDEPLATAFYAASRHIAEMFSRLHYYLENQMLVNIDYADLFNMIKGGNVGILRLLRKVDFNWHWGIWERGLIGILVGNEFPLMNAHRILKNFQEILSEKDIIWGVIIDENLDREVEILSLLVRKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7B2</accession>
    <name>A0A218P7B2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aspartate--tRNA(Asp) ligase</fullName>
            <ecNumber evidence="11">6.1.1.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Aspartyl-tRNA synthetase</fullName>
            <shortName evidence="11">AspRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Discriminating aspartyl-tRNA synthetase</fullName>
            <shortName evidence="11">D-AspRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">aspC</name>
        <name evidence="11" type="synonym">aspS</name>
        <name evidence="13" type="ORF">A3L08_04595</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the attachment of L-aspartate to tRNA(Asp) in a two-step reaction: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Asp) + L-aspartate + ATP = L-aspartyl-tRNA(Asp) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9660"/>
            <dbReference type="Rhea" id="RHEA-COMP:9678"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 3 Mg(2+) cations per subunit. The strongest magnesium site (Mg1) is bound to the beta- and gamma-phosphates of ATP and four water molecules complete its coordination sphere.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.12" evidence="11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853906.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7B2"/>
    <dbReference type="GeneID" id="33315523"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04595"/>
    <dbReference type="OrthoDB" id="5908at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04316">
        <property type="entry name" value="ND_PkAspRS_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000038">
        <property type="entry name" value="Aspartate--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000324">
        <property type="entry name" value="Aspartate--tRNA(Asp/Asn) ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02075">
        <property type="entry name" value="Asp_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
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        <property type="entry name" value="NA-bd_OB-fold"/>
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        <property type="entry name" value="aspS_nondisc"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PRK05159.1"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="9 11" id="KW-0648">Protein biosynthesis</keyword>
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        <location>
            <begin position="137"/>
            <end position="438"/>
        </location>
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            <begin position="192"/>
            <end position="195"/>
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        <location>
            <position position="170"/>
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        <ligand>
            <name>L-aspartate</name>
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        </ligand>
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        <location>
            <begin position="214"/>
            <end position="216"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
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        <ligand>
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        </ligand>
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        <ligand>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
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        <location>
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        </location>
        <ligand>
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        </ligand>
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        <location>
            <position position="364"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
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        <location>
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            <end position="412"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
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        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
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        </source>
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        </source>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ASJ06650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="438" mass="51120" checksum="4B87AB17DC21F5BE" modified="2017-09-27" version="1">MYRTHYSSEITEELNGQRVRVAGWVWEIKDLGGIKFLWLRDREGIVQITAPKKKVDPELFKLIPKLNSEDVVAVEGIVNFTPKAKLGFEILPEKLEILNRAESPLPLDPTGKVKAELDTRLDNRFMDLRRPEVMAIFKIRSSVFKAVRDFFHGEGFIEIHTPKIIATATEGGTELFPMKYFEKDAFLAQSPQLYKQIMMASGLDRVYEIAPIFRAEEHNTTRHLNEAWSIDAEMAFIENEEEVMNLLERLVAYTINYVREHNAKELEILNFELEEPKLPFPRVSYDKALEILSDLGKEIPWGEDIDTEGEKLLGKYMLENEEAPLYFLYQYPSEAKPFYIMKYDDKPEICRAFDLEYRGVEITSGGQREHRVDILEEQIREKGLNPENFEFYLRTFRYGMPPHGGFGLGAERLIKQMLDLNNIREVILFPRDRRRLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7B7</accession>
    <name>A0A218P7B7_9EURY</name>
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        <submittedName>
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        </submittedName>
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        <name evidence="1" type="ORF">A3L08_04685</name>
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        <name type="scientific">Thermococcus pacificus</name>
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            <taxon>Archaea</taxon>
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            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
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                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
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    </dbReference>
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            <dbReference type="EMBL" id="ASJ06668.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="104" mass="11754" checksum="12E9AC06A51852BE" modified="2017-09-27" version="1">MGDKVRRKKYSILAKRIAVVAKSSNGLNEFVEKLLSQMAGDSLFVSREKGENFRNVYKRAKEHEEEVLRFLKDYPYLSTVLYAAYVESISGGGKNEGNIEDEGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7C6</accession>
    <name>A0A218P7C6_9EURY</name>
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        <submittedName>
            <fullName evidence="1">C4-dicarboxylate ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_04790</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
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            <dbReference type="EMBL" id="ASJ06683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="329" mass="34893" checksum="FC98A23F69710B68" modified="2017-09-27" version="1">MKLKAIGLMLVLAFAIVAAGCIQSEGGGTATQTQEITIYTGGTSGVYFPLGSKYAEILNKNGVKAKAVTSGASVSNAKAIGDGTAQAAILQNDVAYYAYNGLYMFEGSKIPNIRGVAALYPETIQFVVRADSDIKTLQDFKGKKVAIGAAGSGTAVAAEQVLKAAGVWDSIEKVNQKFSEAAQSLKLGQVDVAVIVSGAPTPAVNQIAVQTPVRVIPIPDDVLSKLKDQGYIFYVRQVLPKDTYNGMEEDTPTIAVKAMLAVSADLPDDTVYQMTKILFDNLDELHQVHAKTKYISLDTALDGMSIPLHPGAIKYYEEKGISVPAELKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7D5</accession>
    <name>A0A218P7D5_9EURY</name>
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        <submittedName>
            <fullName evidence="8">B12-binding domain-containing radical SAM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_04850</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
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            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7D5"/>
    <dbReference type="GeneID" id="33315574"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04850"/>
    <dbReference type="OrthoDB" id="2305at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02068">
        <property type="entry name" value="radical_SAM_B12_BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.280">
        <property type="entry name" value="Cobalamin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051198">
        <property type="entry name" value="BchE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023980">
        <property type="entry name" value="CHP04013_B12-bd/rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04013">
        <property type="entry name" value="B12_SAM_MJ_1487"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43409">
        <property type="entry name" value="ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43409:SF17">
        <property type="entry name" value="METHYLTHIOTRANSFERASE MJ0865-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="B12-binding" evidence="6">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="160"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="424" mass="48892" checksum="5F300D86191EDA51" modified="2017-09-27" version="1">MPEIAVRMTKRNHNAFVHLLGALESQGFDLSELLITKDFNEILKARPKVVLYSFFTEEIWGSLQEEVRLLKERGALLIAGGYHAIAMPKHTLSHLGFDIAVIGEGEEVLFQLLNVLKRTGYRITKDLLDIRGLAFHINGEFIFTGFAKVEDFWRFPPYPESVRLISPIEITRGCPFGCYYCQTPYIKGLRMRHRPIDQIVKYSRRMRDMRYITPNAFAYGSPGAILKLDKLEALLKALQPLRKEGRRLFYGTFPSEVRPEFVLPETLELLIDYADNRRLAIGAQSGDDAMLRAMHRLHKVEHVQRAVEYMLEYGFEPVVDFIVGLPNESEESQRKSIELMKWIMNKGGKVRAHYFMPLPGTPWSRCKPSPLSPEMKKFLGRMAAKGKIEGSWGKQVELSRRLQRLIDEFYEEPMSHTVPVRNVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7E3</accession>
    <name>A0A218P7E3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Polysaccharide deacetylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_04910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7E3"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04910"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF2334"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10096">
        <property type="entry name" value="DUF2334"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="277" mass="31627" checksum="6055FFB842DD7FB0" modified="2017-09-27" version="1">MFMATVSGIQGEKPLGIPSKFLGNEEPNAEHKPSELGSDITQAQERAELIILVHDVSPVYENELKELLGIISHYGFQNETYLFVIPNHGGKMPINDYPEFVAFLRNLSSEGYHVELHGYDHIGDEFNCGRGEAERRLYLGLKELEDAGFVPEYFIPPRYSLSEDALNVLLSHNLTVIGKDFIYLTNGTMKPIINREYTWYIPRFLVDYQLESAEESYKNTKGTFFLSIHPKAANNEAGLEFLRKFLEFVRKYKTGLGSLESPNGPVVEEHRYQEHSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7G5</accession>
    <name>A0A218P7G5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="15">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">A3L08_05035</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="15"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853978.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7G5"/>
    <dbReference type="GeneID" id="33315611"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05035"/>
    <dbReference type="OrthoDB" id="6009at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.270.10:FF:000011">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1100.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007646">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007647">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019969">
        <property type="entry name" value="RNAP_Rpo2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006335">
        <property type="entry name" value="PRK08565.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007175">
        <property type="entry name" value="PRK09606.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03670">
        <property type="entry name" value="rpoB_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04566">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04567">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 15 23" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="19">
        <location>
            <begin position="28"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="18">
        <location>
            <begin position="175"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="20">
        <location>
            <begin position="424"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="21">
        <location>
            <begin position="521"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="22">
        <location>
            <begin position="605"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="16">
        <location>
            <begin position="653"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="17">
        <location>
            <begin position="1027"/>
            <end position="1116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF04566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF04567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="ASJ06723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="1122" mass="127099" checksum="834B13F42F83C885" modified="2017-09-27" version="1">MASRGPTVVDVTPDDLWLVMEAYWKERGLVRQHLDSYNAFIDYGMQQVIDEFGGVKPDIPDFEVKFGKVRLGEPEFQEAQGQRKPLYPMDARIRNLTYSAPLYLELIPVIKGIEQEPVEVRIGELPIMLKSKACRLYGLSDEELVKLGEDPKDPGGYFIINGSERVIVSIEDLAPNKTLVERDDRQNRIIAKVFSYRHGYRALITVERRKDGILYVNIPNVPRPVKFVYVMRALGLLSDREIVEAVSDDPRIQQVLFDNLEDASDITTQEEALDYIGKLALPGQPKEYRLRRAEHIIDNNLLPHMGVEEKDRRVKAYYLGMMALKVLELSLGLRGEDDKDHYANKRLKLAGDLLRDLFRVAFGQLVKDMQYQMTKTYQRKGERYTFENIQRFVRNSIRPDVLSERIEHALATGAWPGGRTGVSQLLDRTNYMSTLSHLRRVTSPLSRDQPHFEARDLHGTHWGRICPTETPEGPNCGLVKNLALMSQITTGIPEEEVREYLSRLGVVPIEERRPNPKLWRVYLNGVLIGTVEDGESLVGRIRTDRRSGRISDAINVALYQDEEVKEIYVNSDDGRVRRPLIIVENGQPKLTREHVEGIKNGTLTWSDLIKMGVIEYLDAEEEENAYVATWPWEVTEEHTHLELMPAAILGIPASLVPYPEHNAAPRNTYGAGMAKQSLGLGSANFRIRVDTRGHLMHYPQVPLVNSRIMKAVGFEERPAGQNFVVAVLSYGGYNMEDAVIMNKASIERGLARSTFFRTYEAEEKRYLGGQTDRFEVPDPTIQGYLGDKYYRNLDEDGIIFPESKVEGKDVLVGRTSPPRFLEEQSGLGGIILQERRETSVSVRPSERGIVDKVIITETGDGTKLVKVTVRDLRIPEFGDKFASRHGQKGVIGLIVPQEDMPWTESGIVPDLIVNPHGIPSRMTVGQLIEAIGGKVASLKGRRVDGTAFIGEPEEKLRKELEELGFKHSGREVMYDGITGRRLEADVFVGVIYYQRLHHMVADKMHARSRGPVQVLTKQPTEGRAREGGLRFGEMERDVLIGHGAAMLLIERLLEESDKTEVWVCESCGHLALEDKRRGKVYCPVCGEEERISKVEMSYAFKLLLDELKAMVIRPSLNITDRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7K9</accession>
    <name>A0A218P7K9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Metalloprotease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">A3L08_05195</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M50B family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854004.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7K9"/>
    <dbReference type="GeneID" id="33315643"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05195"/>
    <dbReference type="OrthoDB" id="86131at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06158">
        <property type="entry name" value="S2P-M50_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052348">
        <property type="entry name" value="Metallopeptidase_M50B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044537">
        <property type="entry name" value="Rip2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35864">
        <property type="entry name" value="ZINC METALLOPROTEASE MJ0611-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35864:SF1">
        <property type="entry name" value="ZINC METALLOPROTEASE YWHC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 14" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5 14" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="74"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="178"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ06752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="198" mass="22754" checksum="C2503E3246CD61B7" modified="2017-09-27" version="1">MDYRAWKGSRKNELEDFAISFLVLVLLFSNFDPRQLPYALVAVLTAFLSHELAHRQVARRYGYKAYYKRWDTGIMIALLFGLLTRVLTGSTWIFAALGAVYIYTPYQYWEDKEAYGKISLAGPATNIAVGFLSLLILKSLYLSPFAWRVLWTTVVVNLWLAFFNLLPFPPLDGYNVTRWNTGVWAASIGLAYLLYRLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Q0</accession>
    <name>A0A218P7Q0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Peptidase M42</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_05540</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="9">Binds 2 divalent metal cations per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase M42 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854069.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Q0"/>
    <dbReference type="GeneID" id="33315712"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05540"/>
    <dbReference type="OrthoDB" id="30642at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05656">
        <property type="entry name" value="M42_Frv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.40">
        <property type="entry name" value="Peptidase M42, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054931">
        <property type="entry name" value="lys_aminopep_Arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008007">
        <property type="entry name" value="Peptidase_M42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051464">
        <property type="entry name" value="Peptidase_M42_aminopept"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023367">
        <property type="entry name" value="Peptidase_M42_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040822">
        <property type="entry name" value="lys_aminopep_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32481">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32481:SF0">
        <property type="entry name" value="AMINOPEPTIDASE YPDE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05343">
        <property type="entry name" value="Peptidase_M42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001123">
        <property type="entry name" value="PepA_GA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101821">
        <property type="entry name" value="Aminopeptidase/glucanase lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR001123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="345" mass="38116" checksum="99B057D7441D701D" modified="2017-09-27" version="1">MVDIELLRKVVETPGVSGYEFLGIRDVVIEELEPVVDEIYTDKLGNVIAHKKGSGPKIMIAAHMDKIGVMVNHIDKEGYLHVVPVGGVDPRTLVAQRIRFFTEKGERFGVVGHIPPHLQKPEDRKKAADWDTIVVDVGVDSKEEAEELGFRVGTVGEFAPAFVQLNENRIATPYLDDRVCLYTMIEAAKRLESHEADIYFVASVQEEVGLRGARVASYAIDPEIGIAMDVTFAKQVGDKGKIVPRLGGGPVMDVGPNINPKIRAFADEVAKKYNIPLQVEASPRPTGTDANIMQINREGVATAVLSVPIRYMHSQVETADLRDIEKTIEFAKHFLEELKPMDLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7R1</accession>
    <name>A0A218P7R1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Methionine aminopeptidase</fullName>
            <shortName evidence="8">MAP</shortName>
            <shortName evidence="8">MetAP</shortName>
            <ecNumber evidence="8 9">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">map</name>
        <name evidence="11" type="ORF">A3L08_05510</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="8">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase M24A family. Methionine aminopeptidase archaeal type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="8 9"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854064.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7R1"/>
    <dbReference type="GeneID" id="33315706"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05510"/>
    <dbReference type="OrthoDB" id="372008at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004239">
        <property type="term" value="F:initiator methionyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01088">
        <property type="entry name" value="MetAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01975">
        <property type="entry name" value="MetAP_2_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050247">
        <property type="entry name" value="Met_Aminopeptidase_Type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028595">
        <property type="entry name" value="MetAP_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002468">
        <property type="entry name" value="Pept_M24A_MAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018349">
        <property type="entry name" value="Pept_M24A_MAP2_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00501">
        <property type="entry name" value="met_pdase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777:SF2">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01202">
        <property type="entry name" value="MAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="10">
        <location>
            <begin position="6"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="295" mass="32947" checksum="D327EA1F2A2F7145" modified="2017-09-27" version="1">MDEREALIKAGEIARQVKKEVVDLIKPGAKLYDIAEFVERRIVELGGKPAFPCNLSINEIAAHYTPYKGDESVLKEGDYLKVDLGVHVDGYVADTALTFRVGMEEDELMEAAKQALEDAIATVRAGVRISEIGKAIEEAIRGKGFNPIVNLSGHKIERYKLHAGVSIPNVYRPNDTYELKEGDVIAIEPFATTGAGQVIEVPPALIFMYVRDRPVRMAQARRLLMHIKREYNTLPFAYRWLQGFMPEGQLKLALAQLDRVGAIYSYPILREVRGGMVAQFEHTVIVEKDGAYVTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7S2</accession>
    <name>A0A218P7S2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TBP-interacting protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_05565</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854074.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7S2"/>
    <dbReference type="GeneID" id="33315717"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05565"/>
    <dbReference type="OrthoDB" id="84510at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.30">
        <property type="entry name" value="TBP-interacting protein, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.70">
        <property type="entry name" value="TBP-interacting protein, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049118">
        <property type="entry name" value="TBPIP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043112">
        <property type="entry name" value="TIP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029125">
        <property type="entry name" value="TIP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038230">
        <property type="entry name" value="TIP_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21664">
        <property type="entry name" value="TBPIP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15517">
        <property type="entry name" value="TBPIP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159612">
        <property type="entry name" value="TBP-interacting protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TBP-interacting protein N-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="TBP-interacting protein C-terminal" evidence="2">
        <location>
            <begin position="103"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF15517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="224" mass="25956" checksum="C40E15D6CB58FC70" modified="2017-09-27" version="1">MEYGELSPRIKRVYAQVRYLDDYHWEITGDRIIGTHKKSNVKVFIDVADDREHAQKLAEEEKPEGIRIIAIPDKSVFFVHNGAFILTYRYIKATLADINDHIVWSGFKIVEDGGKLVQEDFYEYLGGALINHIKNNMLAGQDYAFWQFYKCEVCGKYVDVESLEGHLKGHGIKHHEKSEEHYEVFEINFQEGKLYDKYGKEIKRDELSEEARDFLDEITAGAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7X7</accession>
    <name>A0A218P7X7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">SAM-dependent tRNA/rRNA cytosine-C5 methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_05850</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854129.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7X7"/>
    <dbReference type="GeneID" id="33315774"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05850"/>
    <dbReference type="OrthoDB" id="14725at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016428">
        <property type="term" value="F:tRNA (cytidine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031341">
        <property type="entry name" value="Methyltr_RsmF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011023">
        <property type="entry name" value="Nop2p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="RsmB/NOL1/NOP2-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00446">
        <property type="entry name" value="nop2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF74">
        <property type="entry name" value="TRNA (CYTOSINE(48)-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17125">
        <property type="entry name" value="Methyltr_RsmF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="8">
        <location>
            <begin position="29"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="120"/>
            <end position="126"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="309" mass="34995" checksum="60D348D99F7A3C88" modified="2017-09-27" version="1">MLEKLFSLGYSKTFAERYYELWGERALAIAEAMEKPLPRCFRVNTLRIEVPKLTKLLNKKGFQFRRVPWAREGFCLTREPFSITSTPEYLSGLLYIQEASSMYPPVALEPKPGETVADMAAAPGGKTSYLAQLMENEGIIYAFDVGEERLKETRLNLSRLGVTNIVLIHSSSLHIDELGVEFDKILLDAPCTGSGTIHKNPERKANRTMDDVKFCQGLQMKLLEKGLSVLRKGGTLVYSTCSLEPEENEFVIQWVLDNFDVELLPLRYGEPALTRPFGIELSEEIAKARRFYPDRHGTSGFFVAKIKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P811</accession>
    <name>A0A218P811_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit Rpo12</fullName>
            <ecNumber evidence="8">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit P</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rpo12</name>
        <name evidence="8" type="synonym">rpoP</name>
        <name evidence="9" type="ORF">A3L08_06045</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the archaeal Rpo12/eukaryotic RPC10 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_014788082.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P811"/>
    <dbReference type="GeneID" id="58918566"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06045"/>
    <dbReference type="OrthoDB" id="129238at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.30">
        <property type="entry name" value="RNA polymerase ii, chain L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00615">
        <property type="entry name" value="RNApol_arch_Rpo12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006591">
        <property type="entry name" value="RNAP_P/RPABC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023464">
        <property type="entry name" value="Rpo12"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001607">
        <property type="entry name" value="PRK00398.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03604">
        <property type="entry name" value="Zn_ribbon_RPAB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00659">
        <property type="entry name" value="RPOLCX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="RNA polymerase subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 8 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 8" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="49" mass="5729" checksum="6C4DB128F75BB39C" modified="2017-09-27" version="1">MVMAVYRCAKCGREVELDLENTREVRCPYCGSKILYKPRPRVARRVKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P833</accession>
    <name>A0A218P833_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NADH dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_06180</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 30 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854187.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P833"/>
    <dbReference type="GeneID" id="33315839"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06180"/>
    <dbReference type="OrthoDB" id="43567at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.80">
        <property type="entry name" value="NADH:ubiquinone oxidoreductase, 30kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037232">
        <property type="entry name" value="NADH_quin_OxRdtase_su_C/D-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001268">
        <property type="entry name" value="NADH_UbQ_OxRdtase_30kDa_su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004738">
        <property type="entry name" value="PRK06074.2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10884:SF14">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10884">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00329">
        <property type="entry name" value="Complex1_30kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143243">
        <property type="entry name" value="Nqo5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase 30kDa subunit" evidence="2">
        <location>
            <begin position="39"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="179" mass="21053" checksum="E1CB9C0FF2B6FA62" modified="2017-09-27" version="1">MAEEVKVTWPKGEEIVSTLLEKAPYAEGKVRRERRLEFRVPADRIVDFLRTAKAHGFELLLQITAVDWPKEGEIELVYQLFSVSHGTHAMVRTRVPRDDARISSVMDIYPVAETYERDAHEFYKVVFEGNPKLSMPWILEDEDREAGVSHRKDFDMLSYVKKKYKILDRFDEDKENYVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P835</accession>
    <name>A0A218P835_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">YgiT-type zinc finger protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05905</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P835"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05905"/>
    <dbReference type="OrthoDB" id="103460at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="82" mass="9383" checksum="F3408761BC7703F1" modified="2017-09-27" version="1">METRKCPLCGGTMIRGKNYQSGYARYFWEAPWKHGIKAALKGPAPAYPWLCLDCGVVLPYVEEAELQRIREEYERAKTGDTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P841</accession>
    <name>A0A218P841_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycosyltransferase RgtA/B/C/D-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_06330</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_157721592.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="33315869"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06330"/>
    <dbReference type="OrthoDB" id="114973at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050297">
        <property type="entry name" value="LipidA_mod_glycosyltrf_83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038731">
        <property type="entry name" value="RgtA/B/C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33908">
        <property type="entry name" value="MANNOSYLTRANSFERASE YKCB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33908:SF11">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13231">
        <property type="entry name" value="PMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="297"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="361"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="390"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="413"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="438"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase RgtA/B/C/D-like" evidence="9">
        <location>
            <begin position="62"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="735" mass="83655" checksum="A30DC568507C0FAA" modified="2017-09-27" version="1">MKDRKKSVMFLSVILSYYLVTRLWGISSAMNEYFDYDEGTYLMIARFINHGVLPYRDIYAVHPPFYYYLLALWLRIFGDSYVVGRLLSVFLGLLAVLVAYLVGRELRDWKTGVLFSAVVVMDPIMVHMNGLVFHETTIELFTMLSLYYFVRYVKHRNRKDALWSLFWVGVGSTSKFTILPYAAALYVVLMLLIDAETESYLDGLGRVLLNRVQVFLVLVAYGIMSLLVVGAVMLYPSDELRRIFILPGVHGIDVVGHIIPAGIFLVIWGFLTLYVFRISYLRKLVRSVHLILRNLRTALQYLLAFLLPKVVIEGILGFGVSGDYLNQTYLAQGSRYIPLAGFFDLFAGIIEDFTNEKPDFIVFYVPLIFIFTVLLFYFGRGEKLERPSVAGPLLIVSSVMYLLAFPVIPNMRFLYPMVLTVYLAFFESLPDRLERKKLIALAFAIALVFGVADYGVAYNYRNGKLLIAWAGHSKDLRDDLGEYIRNTNLHGTYLSINPFNAYYLHLGIDPYCLDTFGIVYMGNSSRLWETANKSDYMLFSTWMYAISRESKVFEKTFGKLKERAVVNGSLLYAESYGKRDVIELFSNSGEKSHVIGFSSFRGKLQLWVNGSEVAYVYPSVGNVSYSWRATIKRNPNGEYDLVYYSKDGNSIAVRLSRDGNSLTLSFPVVVNLTIEFKENAVAIQDGKLLREGTRGKFEIFYPEGSFSVEGNGTIMVVTSSKVVLQCRGVRIEAEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P862</accession>
    <name>A0A218P862_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF2079 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854213.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P862"/>
    <dbReference type="GeneID" id="33315867"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06320"/>
    <dbReference type="OrthoDB" id="44002at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018650">
        <property type="entry name" value="STSV1_Orf64"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09852">
        <property type="entry name" value="DUF2079"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="312"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="502" mass="57821" checksum="FC9F2629CD8614CD" modified="2017-09-27" version="1">MRVKTTYSVWGLILSYSVLLSIIAVNRHYTFRTNAYDLGIFMQAIWSTANGFRVLYNTIEEYQVCVTTHLGVHFSPILLVLVPIYKVFPHAETLLIIQTFVIALSAYPLFLLSKKVLHSERLSLAIVVLYLMNSLLHGINGYDFHATPFALPFIFMTALYFERGEYRKALVSSFFVLLVKEDAGLSILSLSMFYLLRNSKLFSLQTYKSLFEKILTNKLHPSEKTALIMGVLATSWVLISWRVIIPHIAGVHITSAVYYARFPCVEYIPMKILYFIVANLTLGLLTFLRPKYTFLLTFAPWLEILLSCEKNLFRIGFQYPYMILPLSLISVIYTIRELQSNNLKKVFAVSVLVGILFSILTTPILPLDNEIKKDLLVPPHYYQPITHHDRLLLDIIKVVDSTNFSVLTQNDIFPHLANRISTYVIWSSYCGNTLPKTDIILLDNTLLYSVYNYRIKDHLRAYTKVYENDGIEVWIRNDVLETSQATELVRKLQSLERNGKGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P867</accession>
    <name>A0A218P867_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">THUMP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_06400</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854227.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P867"/>
    <dbReference type="GeneID" id="33315883"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06400"/>
    <dbReference type="OrthoDB" id="64018at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002937">
        <property type="term" value="P:tRNA 4-thiouridine biosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11718">
        <property type="entry name" value="THUMP_SPOUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2130.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025849">
        <property type="entry name" value="MJ0421-like_SPOUT_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041730">
        <property type="entry name" value="MJ0421-like_THUMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050102">
        <property type="entry name" value="tRNA_sulfurtransferase_ThiI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43209">
        <property type="entry name" value="TRNA SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43209:SF1">
        <property type="entry name" value="TRNA SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14419">
        <property type="entry name" value="SPOUT_MTase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="2">
        <location>
            <begin position="46"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="350" mass="39731" checksum="0CEF1BD9B4DA9B90" modified="2017-09-27" version="1">MKFIVKTQRGMEGVAANYIREALPNSKVQASPLGYYGLVIVESDDENAVEKILEIPEVERVIPVLVEVPAEIEEIVNAAENVAPFIGENETFAVKTKRRGKHGFSSLDVNRSLGAKVQELTGADVNLSWPDKVVQVEIIGDKAYISVVPGEEFRKFTPDKIDARELFRKLTVVQMPYWGDYKACRSFGEKIGRAAQAFEVKELIIAPKEKMDAFELAEFIKGVKIGQESRHQIQREAYPWKVEKVPVSVWDLYQVVRDKRRAKRLLIITDPKGPTLAEVKDNLARDMFHAKEIIVFVGSREGIPRGLFRFADYVVDLAPYMTFATEHGIPAALVSLWEVYEEYLRSREKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P875</accession>
    <name>A0A218P875_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fe-S cluster assembly protein SufB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_06450</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron-sulfur cluster assembly SufBD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854237.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P875"/>
    <dbReference type="GeneID" id="33315893"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06450"/>
    <dbReference type="OrthoDB" id="372168at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055346">
        <property type="entry name" value="Fe-S_cluster_assembly_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045595">
        <property type="entry name" value="SufBD_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508">
        <property type="entry name" value="FES CLUSTER ASSEMBLY PROTEIN SUF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508:SF1">
        <property type="entry name" value="UPF0051 PROTEIN ABCI8, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19295">
        <property type="entry name" value="SufBD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="Stabilizer of iron transporter SufD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD N-terminal" evidence="3">
        <location>
            <begin position="121"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD core" evidence="2">
        <location>
            <begin position="187"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00043967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="446" mass="50237" checksum="5676E6159AD35348" modified="2017-09-27" version="1">MTETITLSDAKAIIENQIEELAKRNREPEWMTRIRYKALEAFERAPHRDPVISEEELLHFIAKPEIEGLPEKVESLDDLPPEMKALLDRLGISEVEQKYIAGLAVQTDTGVIYNQFLQEWAKKGLIVLPTEEAVRKYPDIVKQHFLKLFRADESKLTAYHTAVWNGGIFLYVKEGLKVPFPLHLFFLIQESALAQAPHIIIIAEKNSEFHLIEGCTAPILVRHSLHLDMTEAYLHDGARAQLTVLQNWPEYVHTRPMTRARIGKGARFINTTVGLGSGRSNVANPKYWVDENGYVELNGILLGQKDWYVDLGGEMYLQGPGAAGINASKAVIMDESRVITRGVIKAEAPKTKGHISCDALLMSDKAVMETYPGLVSRVDDAELSHEAAIGKIREEELFYLMSRGLREEKATQLIVKGFLEPMLKDIPMEFLVEIRRIIELAVSGGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P885</accession>
    <name>A0A218P885_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleotide-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854241.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P885"/>
    <dbReference type="GeneID" id="33315897"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06470"/>
    <dbReference type="OrthoDB" id="147997at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd18677">
        <property type="entry name" value="PIN_MjVapC2-VapC6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39677">
        <property type="entry name" value="RIBONUCLEASE VAPC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39677:SF4">
        <property type="entry name" value="RIBONUCLEASE VAPC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="2"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="143" mass="16176" checksum="833B02AD736586D2" modified="2017-09-27" version="1">MKDLLVDSSVLIEYFKGNERAVKLLESFENADVVLHINDVVFSEVVYILLGHYLGRSPRAMKGNPEKLPPENEEVFKVLKAFGFIPVEQSTVFKAMTLIQKYAMLPNDALILATCVEHDFSLVTLDEDFKLPSEKEGVQTITR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P898</accession>
    <name>A0A218P898_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Coenzyme F420 hydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_06605</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FrhG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854265.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P898"/>
    <dbReference type="GeneID" id="33315924"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06605"/>
    <dbReference type="OrthoDB" id="38261at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.700">
        <property type="entry name" value="NADH:ubiquinone oxidoreductase-like, 20kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051349">
        <property type="entry name" value="Hydrogenase_assoc-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037024">
        <property type="entry name" value="NiFe_Hase_small_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42845">
        <property type="entry name" value="COENZYME F420-REDUCING HYDROGENASE, GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42845:SF2">
        <property type="entry name" value="F420-NON-REDUCING HYDROGENASE VHU SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56770">
        <property type="entry name" value="HydA/Nqo6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="164"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="194"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="230" mass="25519" checksum="411BF2513AC612B3" modified="2017-09-27" version="1">MGRITVLHADFGGCAGCSVSILRSYPEIKDTVKLETPYLVDKYFQSEGYDVAIITGGICVNEGWMLDKLKYIREISDVVVAYGSCAAFGGILRFCRGGQEPRPDHRSFQPINSVIKVDYSIPGCPPAPLMLQSFFRFYLDEDERRLELFRICGSIKKLSGFDLLDDVVLTGLCIGCGACELSCPTHALQVVDKRPNLVQERCIRCGTCYIRCPRATQILTLGGGSRDQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8C0</accession>
    <name>A0A218P8C0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_06410</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854229.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8C0"/>
    <dbReference type="GeneID" id="33315885"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06410"/>
    <dbReference type="OrthoDB" id="57033at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012318">
        <property type="entry name" value="HTH_CRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154">
        <property type="entry name" value="LEUCINE-RESPONSIVE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR AZLB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00034">
        <property type="entry name" value="HTHCRP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="5"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="149" mass="17128" checksum="EB26AF9509925431" modified="2017-09-27" version="1">MADKITSIDIRILKLLSRNARLTYKELAEILGTTRQRISRRMDRLERNGVIKKYTVIPDYDALGYIHVILGITVKPTVNIDEIIHILVEDENVKVVQRALGTHNLVIHVVGPKDMKELEKMIAEVTRKIPEIDKLDITFITDTVKFEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8D2</accession>
    <name>A0A218P8D2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 17">Riboflavin kinase</fullName>
            <shortName evidence="17">RFK</shortName>
            <ecNumber evidence="4 17">2.7.1.161</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14 17">CTP-dependent riboflavin kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15 17">CTP:riboflavin 5'-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13 17">Flavokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ribK</name>
        <name evidence="19" type="ORF">A3L08_06650</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 17">Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>riboflavin + CTP = CDP + FMN + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25021"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.1.161"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="17">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 17">Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the archaeal riboflavin kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.161" evidence="4 17"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854274.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8D2"/>
    <dbReference type="GeneID" id="33315933"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06650"/>
    <dbReference type="OrthoDB" id="30955at2157"/>
    <dbReference type="UniPathway" id="UPA00276">
        <property type="reaction ID" value="UER00929"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008531">
        <property type="term" value="F:riboflavin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009398">
        <property type="term" value="P:FMN biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.30">
        <property type="entry name" value="Riboflavin kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01285">
        <property type="entry name" value="Riboflavin_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012318">
        <property type="entry name" value="HTH_CRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039063">
        <property type="entry name" value="RibK_CTP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023470">
        <property type="entry name" value="Riboflavin_kinase_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023602">
        <property type="entry name" value="Riboflavin_kinase_CTP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023465">
        <property type="entry name" value="Riboflavin_kinase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40706">
        <property type="entry name" value="RIBOFLAVIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40706:SF1">
        <property type="entry name" value="RIBOFLAVIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01982">
        <property type="entry name" value="CTP-dep_RFKase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13545">
        <property type="entry name" value="HTH_Crp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00419">
        <property type="entry name" value="HTH_CRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82114">
        <property type="entry name" value="Riboflavin kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51063">
        <property type="entry name" value="HTH_CRP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 17" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 17" id="KW-0288">FMN</keyword>
    <keyword evidence="11 17 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HTH crp-type" evidence="18">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="95"/>
            <end position="100"/>
        </location>
        <ligand>
            <name>CDP</name>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="191"/>
            <end position="194"/>
        </location>
        <ligand>
            <name>CDP</name>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00033116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ASJ07024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="212" mass="23715" checksum="40F08CC96E5B7843" modified="2017-09-27" version="1">MKRVKMLILLARRDAIGERVKITMRELADELGISPQSVLRLLDEMEGDGLIERFVEGKKTYVEITPKGLAFLEDLCDAISEVLYNGVIIGEVISGIGEGAYYVRQYSHLIKEYLGFEPYPGTLNVKVIFPKTVFDALCNVRPVILPGFVREGRTFGDVKAYRVRIGNVEGAIVIPSRTVHPPKIAEIVAPVYLRKELGLRDGSKITIRVVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8G1</accession>
    <name>A0A218P8G1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">IMP cyclohydrolase</fullName>
            <ecNumber evidence="3 4">3.5.4.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">IMP synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Inosinicase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">purO</name>
        <name evidence="6" type="ORF">A3L08_06915</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the cyclization of 5-formylamidoimidazole-4-carboxamide ribonucleotide to IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:18445"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="EC" id="3.5.4.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal IMP cyclohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.10" evidence="3 4"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854322.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8G1"/>
    <dbReference type="GeneID" id="33315986"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06915"/>
    <dbReference type="OrthoDB" id="92928at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00135"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.20">
        <property type="entry name" value="Inosine monophosphate cyclohydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00705">
        <property type="entry name" value="IMP_cyclohydrol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010191">
        <property type="entry name" value="IMP_cyclohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020600">
        <property type="entry name" value="IMP_cyclohydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036795">
        <property type="entry name" value="IMP_cyclohydrolase-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003167">
        <property type="entry name" value="PRK04151.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01922">
        <property type="entry name" value="purO_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07826">
        <property type="entry name" value="IMP_cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004866">
        <property type="entry name" value="IMP_cclhdr_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75569">
        <property type="entry name" value="Archaeal IMP cyclohydrolase PurO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inosine monophosphate cyclohydrolase-like" evidence="5">
        <location>
            <begin position="3"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="NCBIfam" id="TIGR01922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="197" mass="22257" checksum="A0966ED41808823E" modified="2017-09-27" version="1">MAYTGRTLGIGLMNGKPFAFYLLCSRSFPNRKAVIRGNAAYIINQTETDNPYVSYPVVRVNERYAVVTNGLHTDFIFQALEWEKPRKALIHVLDAMDYERDDHNTPRIAGVVERGSERGWLGFVGRDGFWVRELELREGKALVTATYNVDGFIKIGLSNFSSARELATEILKLPFEHKIISIGIAEKKDGWEIEAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8K7</accession>
    <name>A0A218P8K7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Xaa-Pro dipeptidase</fullName>
            <ecNumber evidence="8">3.4.13.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Imidodipeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Proline dipeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">A3L08_07215</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Splits dipeptides with a prolyl in the C-terminal position and a nonpolar amino acid at the N-terminal position.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Xaa-L-Pro dipeptide + H2O = an L-alpha-amino acid + L-proline</text>
            <dbReference type="Rhea" id="RHEA:76407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:195196"/>
            <dbReference type="EC" id="3.4.13.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the peptidase M24B family. Archaeal-type prolidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.13.9" evidence="8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8K7"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07215"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102009">
        <property type="term" value="F:proline dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="APP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Aminopeptidase P family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
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        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
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    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
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    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050659">
        <property type="entry name" value="Peptidase_M24B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053500">
        <property type="entry name" value="Prolidase_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040821">
        <property type="entry name" value="dipep_PepQ_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46112">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46112:SF2">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0170">Cobalt</keyword>
    <keyword evidence="5" id="KW-0224">Dipeptidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase N-terminal" evidence="17">
        <location>
            <begin position="2"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24" evidence="16">
        <location>
            <begin position="132"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ASJ07125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="348" mass="39247" checksum="11B85BE6846027AB" modified="2017-09-27" version="1">MRIEKLREFIAEKELDGVLIRAHENLFYFTGSAPVLGGYLVVTPDEATFIVPELEYEEAKETSKVPVEKFKTGKELYERLSSLKLRKLGIEGKTSFSTIQTLKEKANVEDFTPIDDVIRELRIIKTKEELEVIEAACKIADMAMMAALEEISEGKREREIAAKMEYVMKMNGAEKPAFDTIIASGWRSALPHGVASDKRIEKGDFVVIDEGALYRHYHSDMTRTIVVGSPNEKQKEIYEIVLEAQKKGVEAARPGMTAKELDTIVRDIIAEYGYGDHFIHSTGHGVGLEIHEWPRVSQFDETVLKPGMVITIEPGIYLPKFGGVRIEDTIVITENGARRLTKTERELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8K8</accession>
    <name>A0A218P8K8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Rubrerythrin family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_07195</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8K8"/>
    <dbReference type="GeneID" id="33316042"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07195"/>
    <dbReference type="OrthoDB" id="42847at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
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    </dbReference>
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    </dbReference>
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        <property type="term" value="P:intracellular manganese ion homeostasis"/>
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    </dbReference>
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        <property type="entry name" value="Ferritin_CCC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01044">
        <property type="entry name" value="Ferritin_CCC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ccc1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039376">
        <property type="entry name" value="Ferritin_CCC1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rr_diiron-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31851">
        <property type="entry name" value="FE(2+)/MN(2+) TRANSPORTER PCL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01988">
        <property type="entry name" value="VIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Rubrerythrin diiron-binding" evidence="6">
        <location>
            <begin position="13"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="364" mass="40179" checksum="255073628C1020D0" modified="2017-09-27" version="1">MDEMLKLARGFYRDEYADSVLYAQLAKTEKDARIREEFLRLSNIESKHAKFWHDFVRARGGEVPKPKVRKLSILSTKLLRKLLGPGAVASLLEMGENSAIQKYFKYLTTYADKFTDEEMVRIKDVILDELEHERFFYESKKRFHVENIRDFVLGMNDGLVEILGAVTGLSAVYPDSPQLVGISGLIVGVAGALSMAIGAFISVRSQRQVNEAVQGRMEVLFKVSPGRVKEELVSRLVEGGVPKDVAEKTAEELAQKGDAVMRLLIQETDENELRSALYTGLAYLLGVVFPVTPYFLASSSLTALPLSVLFAGTALAVVASLISLLSGISVRKKVVEMVTTGLGAAFLSYLFGRAMEALFHVSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8P1</accession>
    <name>A0A218P8P1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_07315</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854395.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8P1"/>
    <dbReference type="GeneID" id="33316066"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07315"/>
    <dbReference type="OrthoDB" id="24644at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03235">
        <property type="entry name" value="ABC_Metallic_Cations"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050153">
        <property type="entry name" value="Metal_Ion_Import_ABC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42734">
        <property type="entry name" value="METAL TRANSPORT SYSTEM ATP-BINDING PROTEIN TM_0124-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="264" mass="28930" checksum="CC5ABEF946CE8DF6" modified="2017-09-27" version="1">MNAVTAENLTILYDGKPALEGVTFSLEEGETLLLLGPNGAGKTTLLRTIACFHREYTGDLYVFGRKPCRARELIGYVPQSHSLNERVPLTALEVVAMGGIYRRGFAHFKIPRETLLKAEEVLGFVGLGSVKDRLFRELSGGQKQRVLLARALMGDPKLLLLDEPLSALDPSARAEVAAVLNKIKRERGITMVITTHDINPLLEVGDRVMLINKRLIAFGRPEEVLQDSIIKSVYGPLARVVPVGGKLFCITGDVHIHKRGGGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8P8</accession>
    <name>A0A218P8P8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Large ribosomal subunit protein eL37</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 13" type="primary">rpl37e</name>
        <name evidence="13" type="ORF">A3L08_07185</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11 12">Belongs to the eukaryotic ribosomal protein eL37 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854371.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8P8"/>
    <dbReference type="GeneID" id="33316040"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07185"/>
    <dbReference type="OrthoDB" id="5619at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.30:FF:000003">
        <property type="entry name" value="50S ribosomal protein L37e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00547">
        <property type="entry name" value="Ribosomal_eL37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001569">
        <property type="entry name" value="Ribosomal_eL37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018267">
        <property type="entry name" value="Ribosomal_eL37_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003214">
        <property type="entry name" value="PRK04179.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01907">
        <property type="entry name" value="Ribosomal_L37e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01077">
        <property type="entry name" value="RIBOSOMAL_L37E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11 12" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8 11 12" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="7 11 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 11 12" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="6 11 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="zinc finger region" description="C4-type" evidence="11">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00035225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ07120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="63" mass="7500" checksum="C6FD1B091BB73E42" modified="2017-09-27" version="1">MGAGTEPKGRRNHTPTHIRCRRCGRRAFNAKKGYCAACGFGRSRRMRKYSWSHKWKKKRNLKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Q3</accession>
    <name>A0A218P8Q3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein archease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_07535</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Activates the tRNA-splicing ligase complex by facilitating the enzymatic turnover of catalytic subunit RtcB. Acts by promoting the guanylylation of RtcB, a key intermediate step in tRNA ligation. Can also alter the NTP specificity of RtcB such that ATP, dGTP or ITP is used efficiently.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the archease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854432.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Q3"/>
    <dbReference type="GeneID" id="33316110"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07535"/>
    <dbReference type="OrthoDB" id="8831at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.55.10.10:FF:000002">
        <property type="entry name" value="Archease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.10.10">
        <property type="entry name" value="Archease domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01222">
        <property type="entry name" value="Archease_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002804">
        <property type="entry name" value="Archease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022952">
        <property type="entry name" value="Archease_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023572">
        <property type="entry name" value="Archease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036820">
        <property type="entry name" value="Archease_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001617">
        <property type="entry name" value="PRK00407.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12682">
        <property type="entry name" value="ARCHEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12682:SF11">
        <property type="entry name" value="PROTEIN ARCHEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01951">
        <property type="entry name" value="Archease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69819">
        <property type="entry name" value="MTH1598-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Archease" evidence="7">
        <location>
            <begin position="4"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="142" mass="16400" checksum="91C97BF35110CBE1" modified="2017-09-27" version="1">MREWEHYEHTADIGIRGYGSTLEEAFEAVALALFDVMVNVRKVEPRECREVEVEGEDLMALLYNFLEELLVLHDMEGLVFGDVKVEIEESGDGYKLKARACGEPLDYEKHEPKEEVKAITYHDMEIKQLPDGRWMAQLVPDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8T6</accession>
    <name>A0A218P8T6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8T6"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07555"/>
    <dbReference type="OrthoDB" id="86287at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43471">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43471:SF13">
        <property type="entry name" value="ABC-2 TYPE TRANSPORT SYSTEM PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12679">
        <property type="entry name" value="ABC2_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="308" mass="33915" checksum="11B8787D3A172DA9" modified="2017-09-27" version="1">MNPAWNIALKELYTSVKSRRFGVIVAIYFIIFGLAVYAIKDYLIQMGVPGVESNELYLWGVNAEVYTTPFAMLFTVNMMIITVLGAVLGAALGADAINREVENGTAKVLLGHPVYRDEVINGKFLGIGTLIAVTYLVSYVVMVAVMLILGIPLDGDSLLRGFLATLVTILYTLVFLSLGVLFSTLSKKPETSMLAAVGLAIFLTVFYGIVVGIVAPKFVGPEPPWGTTAHGMWEDELNTWMARLHSINPAHHYVQLVHYIFGGDRFMNYYIPLSDSFTYGFNNLAALMIMLLLPFALAYARFLTSDLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8T8</accession>
    <name>A0A218P8T8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">TldD-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_07730</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U62 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854463.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8T8"/>
    <dbReference type="GeneID" id="33316148"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07730"/>
    <dbReference type="OrthoDB" id="98233at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2290.10:FF:000003">
        <property type="entry name" value="Zinc-dependent protease, TldD/PmbA family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="entry name" value="PmbA/TldD superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051463">
        <property type="entry name" value="Peptidase_U62_metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025502">
        <property type="entry name" value="TldD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30624:SF0">
        <property type="entry name" value="METALLOPROTEASE SLR0863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30624">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN TLDD AND PMBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004919">
        <property type="entry name" value="TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="Putative modulator of DNA gyrase, PmbA/TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloprotease TldD/E N-terminal" evidence="5">
        <location>
            <begin position="21"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloprotease TldD/E central" evidence="7">
        <location>
            <begin position="112"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloprotease TldD/E C-terminal" evidence="6">
        <location>
            <begin position="229"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="455" mass="50879" checksum="BF8CB97359C77F21" modified="2017-09-27" version="1">MEALERALKWAEENLKAEYIELRYEDLKKTTLGLKDGVFTSFTGKLHRGVAIRVLADGAWGFSSTSDLSSLEKKIEEAYKLAKAAAQTKREKIELAEIKPVEDFVKSKMRVKPREVDIEEKVNHLRELEKLLKEDSAVKSVQIRYEDGGGRKLLLTNEGTRIEWDYNYLYQGTYVTGKADGKLAMARDSIGAVDYGWELMTDHEPNEKVTERILRKMHSQLKGVAPKRGEWPIVAGPIVVGIIAHEALGHLAEADLTINSPFKDLIGKQIAPEYVTMSERYVEGGFGNDKYDDEGVPVKDIHIIENGILKEIMLNREYAAKWGMEPNGHARAESYRYAPIIRMRNTIFEPGDHSFEELIEDIKFGYYVVDFRGGQAQLNSAFQVGIQEGYVIRNGEIAEPIRDTSITGVAIEALKKISAVGKDFGLEVGFCGKGQTAFVSSGGPHMRFDGGILIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Z6</accession>
    <name>A0A218P8Z6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphatidic acid phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_07890</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854494.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Z6"/>
    <dbReference type="GeneID" id="33316183"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07890"/>
    <dbReference type="OrthoDB" id="10182at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01610">
        <property type="entry name" value="PAP2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF13">
        <property type="entry name" value="AT30094P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="2">
        <location>
            <begin position="81"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="216" mass="24291" checksum="C517DF6DF201FD91" modified="2017-09-27" version="1">MAKSEHKRGFLALTLALFALLTLQITGAFSGVNEWVNSVLPSGGPLTSAFTDTASFALTAVYILLFLLWDFKERGRLSRFTLELTAGIAVSMVIVGLLKVLTSVPRPGEAQVHWSLFEAIKNADYFAFPSGHTTRAAVLAYFLSRRWKRLWPLWWGWALGVGLSRLFLHAHWFSDVLFSAFLGPWVALLVEMTEEWWLPYYRAVVNALKLGVLNVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P937</accession>
    <name>A0A218P937_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NAD(P)H nitroreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_08130</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the nitroreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854535.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P937"/>
    <dbReference type="GeneID" id="33316232"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08130"/>
    <dbReference type="OrthoDB" id="105365at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02151">
        <property type="entry name" value="nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="entry name" value="NADH Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43673">
        <property type="entry name" value="NAD(P)H NITROREDUCTASE YDGI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43673:SF10">
        <property type="entry name" value="NADH DEHYDROGENASE_NAD(P)H NITROREDUCTASE XCC3605-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="FMN-dependent nitroreductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="3">
        <location>
            <begin position="7"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="179" mass="20474" checksum="9D1F7803231D90A2" modified="2017-09-27" version="1">MEFFEVLRRRRSVRRFQDKPVPEELVEKLLEAAFLSPSSFNKRPWHFIVVDDKEKLKALSKAKLGASGLATAPLAIVITADESRSDVWIEDASIAAEHIHLASFALGLSSFWVQIRNRVHSEDKTAEDYVRELLNIPENYRVLCIIGIGYPAEKKPPHGDEVFEWEKASKNEFGRPFKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P940</accession>
    <name>A0A218P940_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carbohydrate kinase PfkB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_08210</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P940"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08210"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01166">
        <property type="entry name" value="KdgK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050306">
        <property type="entry name" value="PfkB_Carbo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43085">
        <property type="entry name" value="HEXOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43085:SF1">
        <property type="entry name" value="PSEUDOURIDINE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="6">
        <location>
            <begin position="2"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="286" mass="31374" checksum="A0014A12C25CB186" modified="2017-09-27" version="1">MLDVLSIGEVLVDLKIIDGRISMHTGGSCLNVSFYSRQAGAKSSFIGTVGDDFLGAHIKKELKELDFKPALSVVDHNTTLVLIKAERETPIPIIYRGADRFIKKEQLEEKWKDARIVHTSAFALSLSPAKEMILEALKSAKENGALISVDPNYRRWIWKKWEADEKALLEAISLADLVKPSLDDAKELLGIEDPIDILRRFKEMGAKNVILSMGSKGVIALTEEGRYIRVPAEKAELVDPTGAGDSLLGTVLAKLSRGHDMEEAIRSGVEVASKVVSAEGTLVKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P954</accession>
    <name>A0A218P954_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF302 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P954"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08180"/>
    <dbReference type="OrthoDB" id="2559at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd14797">
        <property type="entry name" value="DUF302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.70">
        <property type="entry name" value="TT1751-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005180">
        <property type="entry name" value="DUF302"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035923">
        <property type="entry name" value="TT1751-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016796">
        <property type="entry name" value="UCP021774"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38342">
        <property type="entry name" value="SLR5037 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38342:SF1">
        <property type="entry name" value="SLR5037 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03625">
        <property type="entry name" value="DUF302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF021774">
        <property type="entry name" value="UCP021774"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103247">
        <property type="entry name" value="TT1751-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF302" evidence="1">
        <location>
            <begin position="38"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="117" mass="13643" checksum="0FCC855269ECD9E9" modified="2017-09-27" version="1">MFRYRRKLDMGLKEAEEKFKAKLEEKGYKVVLEFTPSDVVKAKLGIEMEPYRILYVCNPKKFYEMTSVEYEIGSFAPCPVLLYEKDGEVYISINTAEDVVEIIKDALEDVRSVIEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P988</accession>
    <name>A0A218P988_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854601.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P988"/>
    <dbReference type="GeneID" id="33316301"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08475"/>
    <dbReference type="OrthoDB" id="65817at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43534:SF1">
        <property type="entry name" value="4FE-4S CLUSTER CONTAINING PARA FAMILY ATPASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43534">
        <property type="entry name" value="MIND SUPERFAMILY P-LOOP ATPASE CONTAINING AN INSERTED FERREDOXIN DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="62"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="92"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="296" mass="32120" checksum="83E43C1957904E10" modified="2017-09-27" version="1">MQIAIASGKGGVGKSTITASLLYFLKDEYKLIAVDADAEAPNLGLLLGVIEWEEEREHIGAKVARINTESCIRCGICMERCPYDSIYIDDEGNYVVNELTCEGCNVCGLVCPVPGTIALEEVRSGVVRKATTKYGFPIISAQLDVGRPESGKLVTEEKEWAKKIMEELNLEHMVVDSAAGIGCQVIASLGGADVAILIAEPTPASLSDVQRAYKVVQHFREPAYLIINKADINPGFTKLREWAEAEGIPILGEVPYDRAIPESMVMLKPVVEAFPESKAAKALREIAERIKGEIIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P997</accession>
    <name>A0A218P997_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MIP18 family-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854595.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P997"/>
    <dbReference type="GeneID" id="33316295"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08445"/>
    <dbReference type="OrthoDB" id="85788at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIP18 family-like" evidence="1">
        <location>
            <begin position="5"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="95" mass="10733" checksum="942E2E8B8889A57B" modified="2017-09-27" version="1">MGIEEEAIKRLRNVKDPVTEENIVDLGMVVSIVEDEDGVRLYLDLSRGTHGPFMDALTWPVRAKIVRDAVAVLSDLGKVEILDAKSFQRYYPEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P998</accession>
    <name>A0A218P998_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tetrahydromethanopterin S-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854608.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P998"/>
    <dbReference type="GeneID" id="33316311"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08530"/>
    <dbReference type="OrthoDB" id="102540at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="63" mass="7263" checksum="B5D4E449100501F8" modified="2017-09-27" version="1">MVRGVIPDQTTIKRSEMLAKEKTERYKHEAREVNDYSKLETAIVLVLGIIVIGFVIFVLSHYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P999</accession>
    <name>A0A218P999_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08545</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854611.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P999"/>
    <dbReference type="GeneID" id="33316314"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08545"/>
    <dbReference type="OrthoDB" id="371648at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11378">
        <property type="entry name" value="DUF296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.80">
        <property type="entry name" value="Hypothetical protein, similar to alpha- acetolactate decarboxylase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025707">
        <property type="entry name" value="DNA_bp_PD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005175">
        <property type="entry name" value="PPC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34988:SF1">
        <property type="entry name" value="DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34988">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03479">
        <property type="entry name" value="PCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016702">
        <property type="entry name" value="DNA_bp_PD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117856">
        <property type="entry name" value="AF0104/ALDC/Ptd012-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51742">
        <property type="entry name" value="PPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPC" evidence="1">
        <location>
            <begin position="3"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="140" mass="15348" checksum="A3B51A8DA89040A0" modified="2017-09-27" version="1">MEFKPGKVFLMRAPEGVDLLEFVNSFAEKNGINTAIVKGIGSLRNPVVGYYSEETKSYKKIELVGTFELLTLLGNISIKDGKPFAHLHVTLGNANGDVFGGHLIKGAVFVAEVFIQELLGEPLERKKQENGLVLWDSEGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9A3</accession>
    <name>A0A218P9A3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Inosine-5-monophosphate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_08580</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854617.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9A3"/>
    <dbReference type="GeneID" id="33316321"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08580"/>
    <dbReference type="OrthoDB" id="43333at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044065">
        <property type="entry name" value="ACP_MB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51901">
        <property type="entry name" value="ACP_MB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="11"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="76"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="ACP-type MB" evidence="5">
        <location>
            <begin position="141"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="177" mass="19733" checksum="5B1AEE8BD276A2C1" modified="2017-09-27" version="1">MAPRIAVGQVVKRKAVIVKPDDTVHRVARILSRNKVGSAVVVKDDEIVGIITDRDILDKVVARGRDPKTVKVEEVMTKNPITIEDDYEVQDAIDKMMDKGIRRLLVTRLGKPVGFVTAADLLAALNTYNHEEEEESEGETEVYGICEICGQYGPLYKVYIEGGEKWICESCKDSLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9B7</accession>
    <name>A0A218P9B7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_08630</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854624.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9B7"/>
    <dbReference type="GeneID" id="33316332"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08630"/>
    <dbReference type="OrthoDB" id="57329at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08179">
        <property type="entry name" value="NADPH_BDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1970:FF:000003">
        <property type="entry name" value="Alcohol dehydrogenase, iron-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1090.10:FF:000001">
        <property type="entry name" value="Aldehyde-alcohol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056798">
        <property type="entry name" value="ADH_Fe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039697">
        <property type="entry name" value="Alcohol_dehydrogenase_Fe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034802">
        <property type="entry name" value="NADPH_BDH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496:SF83">
        <property type="entry name" value="HYDROXYACID-OXOACID TRANSHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25137">
        <property type="entry name" value="ADH_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA" evidence="2">
        <location>
            <begin position="6"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Fe-containing alcohol dehydrogenase-like C-terminal" evidence="3">
        <location>
            <begin position="191"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="376" mass="41698" checksum="5C6454BB21FF3D5D" modified="2017-09-27" version="1">MFWLKTRIIEGEGSLKALSEEARGYERVLILASNSMKRHGFLSEAEDYVREAGAEVLSITGLPAEPSVETVEELLPKLREEFQPDLLVALGGGSVIDTAKALKVFYDAPEVKFEEIAFIDRFSKPKPVPKLRTPLIAIPSTSGAGSEVSAASVLKRGDVKYNIVTPEITPEVAILDPRLPRTMPREVARNSGLDVLVHGIEAYTTKAANPFSDAMAIQAIKAVYKWLPLSVKGDEEARARVHYAATMAGIAFLNARLGLAHAMSHKAAWIGPHGLLNAIFIPYVMEFNAERSEYARKRYAEIARELGFKTAKDLIEVVRELNEMLGVPRLRDLVDEETFMAKLDEMAEKAYRDGLIAFNPVEPTPEEIKELYLKAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9G5</accession>
    <name>A0A218P9G5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08795</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9G5"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08795"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="184" mass="20394" checksum="11DBD462B308B902" modified="2017-09-27" version="1">MAGAGLAASGGLLPFLLSLALVAGFNFLSSRDPMRALKAVPCGETLRTLVNGREIDCLTDNVSYGGYRGRNTLVVGGKFVKEFPRWREVVECLLRVPYSETPQKILGYSSSFLMVAVGFVLPPGLFAIAVLYALAFVMMMGVAAYLVRRTKKHIPEDCKEVMKEYAEFYRKKAKERDKRSIVID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9H7</accession>
    <name>A0A218P9H7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NYN domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854668.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9H7"/>
    <dbReference type="GeneID" id="33316380"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08870"/>
    <dbReference type="OrthoDB" id="85234at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18726">
        <property type="entry name" value="PIN_LabA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002790">
        <property type="entry name" value="CHP00288"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021139">
        <property type="entry name" value="NYN"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00288">
        <property type="entry name" value="TIGR00288 family NYN domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35811:SF1">
        <property type="entry name" value="HTH OST-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35811">
        <property type="entry name" value="SLR1870 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01936">
        <property type="entry name" value="NYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NYN" evidence="1">
        <location>
            <begin position="32"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="170" mass="18559" checksum="D4169E70F83FF601" modified="2017-09-27" version="1">MAGSNWERIISMTKDGMRSIGMMRRKISRGKRIALLIDGPNILRKEFGIKLEDIVEALEGLGDLRVAKVILNQYAPQGLIEAVSNQGFDVIVVSGETGVKLAVEAMREIYNPNIDAIALATRNAEFLPVILKAKEKGKETIVLGVEPGFSAALKHAADYTIILNPEGDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9I6</accession>
    <name>A0A218P9I6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">AsnC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_08960</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854683.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9I6"/>
    <dbReference type="GeneID" id="33316398"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08960"/>
    <dbReference type="OrthoDB" id="6762at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019885">
        <property type="entry name" value="Tscrpt_reg_HTH_AsnC-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154">
        <property type="entry name" value="LEUCINE-RESPONSIVE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154:SF50">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00519">
        <property type="entry name" value="HTH_ASNC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="4"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="157" mass="17978" checksum="C50B12605BDE1C56" modified="2017-09-27" version="1">MPGVDEKDREILRILRKEGRITLTELGKRVNLSPASVKNRVEKLEKLGAIRGYSAVVNPTFLGEYVSVFLELKLAIDDHTVDPILRKVAGMENVEAVYRRSGERQILVKAHFQDTDEVKAFAGKLKRLFGRNLERVEATLVIDTFKESWIDLERKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9N3</accession>
    <name>A0A218P9N3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">3-methyladenine DNA glycosylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_09150</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854717.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9N3"/>
    <dbReference type="GeneID" id="33316436"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09150"/>
    <dbReference type="OrthoDB" id="8200at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032993">
        <property type="term" value="C:protein-DNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032131">
        <property type="term" value="F:alkylated DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008725">
        <property type="term" value="F:DNA-3-methyladenine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043916">
        <property type="term" value="F:DNA-7-methylguanine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006307">
        <property type="term" value="P:DNA alkylation repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051912">
        <property type="entry name" value="Alkylbase_DNA_Glycosylase/TA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43003">
        <property type="entry name" value="DNA-3-METHYLADENINE GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43003:SF5">
        <property type="entry name" value="DNA-3-METHYLADENINE GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="2" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="3">
        <location>
            <begin position="114"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="291" mass="33216" checksum="AC0BE1C6F74A7197" modified="2017-09-27" version="1">MVEVDIQKTAHEMIRNGTWKFEGGIFYQALRLPGGKAVVAGYNGEDFIIPEDLDDGDKRLIEERLFFILGLGTDLDSFYAEISDSPFAFLAEEFRGLTVPAAPSPYQALMEAIAQQQVSFDFAQRTIANLVMLAGKRVGEVYAFPRPEEIAQLGEEKLREAKLGYRAQYIKNLTALYLEGKLGLDLWGWGVEDAIKYLTKFRGIGKWTAELFLAYGLRKNVYPAGDLGLRRGMAKIFGKRVKEVKERDVREVIEPYGKWKSLLAFYVTCYDRKTELERKVKIQNRRKNSGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9P2</accession>
    <name>A0A218P9P2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_09340</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854750.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9P2"/>
    <dbReference type="GeneID" id="33316474"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09340"/>
    <dbReference type="OrthoDB" id="86308at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049295">
        <property type="entry name" value="DUF6849"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20986">
        <property type="entry name" value="DUF6849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CDC48" evidence="3">
        <location>
            <begin position="12"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="142" mass="15932" checksum="1FBFCFC1964B1B79" modified="2017-09-27" version="1">MRLILKPLFDSELSAGFEEIVRTKLIGREVRTGEKIEITLLGEPLHFKVLLAEPSPMKVDKTTKIEFSFGDIDVVNVEFEKPVDDVIPTGRGFVVILGREIVVLSKDGQKLYSEEFKELKGIRVTEDSVVVIHGDGVKIIRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Q6</accession>
    <name>A0A218P9Q6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphate-starvation-inducible E-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_09445</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854769.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Q6"/>
    <dbReference type="GeneID" id="33316495"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09445"/>
    <dbReference type="OrthoDB" id="86341at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020948">
        <property type="entry name" value="P_starv_induced_PsiE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06146">
        <property type="entry name" value="PsiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="17803" checksum="14BB439EC1BFBDFE" modified="2017-09-27" version="1">MAIIPKPEKELTPLESIVLKWMNLSFDFVVIVLALLTMAYVIYAIYDLIGILFHGFDVEEALHEFLLVIILLELFELLTLYIKEHHVSMRRVAELGVVALVRKLVITVDYKSLGWEVMLGMAALIFVLGWIYVQERWRVSEEEKFIVEHGLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Q8</accession>
    <name>A0A218P9Q8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein eL31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl31e</name>
        <name evidence="6" type="ORF">A3L08_09500</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL31 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854780.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Q8"/>
    <dbReference type="GeneID" id="33316506"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09500"/>
    <dbReference type="OrthoDB" id="10127at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.440.10:FF:000005">
        <property type="entry name" value="50S ribosomal protein L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00410">
        <property type="entry name" value="Ribosomal_eL31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000054">
        <property type="entry name" value="Ribosomal_eL31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020052">
        <property type="entry name" value="Ribosomal_eL31_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023621">
        <property type="entry name" value="Ribosomal_eL31_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002258">
        <property type="entry name" value="PRK01192.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01198">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01380">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54575">
        <property type="entry name" value="Ribosomal protein L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01144">
        <property type="entry name" value="RIBOSOMAL_L31E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="90" mass="10389" checksum="EE359C90D58071BC" modified="2017-09-27" version="1">MPIKPGEEVIFVVPIQKIKKRVPRWKRAPRAARFLREWIARHAKADEVIIGTDVNEKIWERGAEKPPSKLRVKVVVEEEEGKRVAKVSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9V3</accession>
    <name>A0A218P9V3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2-haloalkanoic acid dehalogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_09715</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_198362124.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9V3"/>
    <dbReference type="GeneID" id="33316549"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09715"/>
    <dbReference type="OrthoDB" id="27736at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011950">
        <property type="entry name" value="HAD-SF_hydro_IA_CTE7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051540">
        <property type="entry name" value="S-2-haloacid_dehalogenase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02253">
        <property type="entry name" value="CTE7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43316:SF3">
        <property type="entry name" value="HALOACID DEHALOGENASE, TYPE II (AFU_ORTHOLOGUE AFUA_2G07750)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43316">
        <property type="entry name" value="HYDROLASE, HALOACID DELAHOGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01135">
        <property type="entry name" value="C1.5.6:_HAD__Beta-PGM__Phospha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="236" mass="26451" checksum="761FBDD24E4EE2A6" modified="2017-09-27" version="1">MLKAVFLDFVGTLITKAGENVTHQNIVREVLREAGREDLDYIKIWEEYEGESSALFKELAGKPYVKIREVDTKAMQKVAERHGFEVPENFWEISIAMHERFGELFPDAVGTIKALKDMGLHVGIITDSDNDYIEAHLKALGIYDLFDSITTSEDAGFYKPHERPFILALERAGVKPEEAIYVGDNPAKDCVGARNVGMLSVLVDSDGKKRELWGNCDFVVSKLSDVVEIVKGLVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9W3</accession>
    <name>A0A218P9W3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">A3L08_09645</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854806.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9W3"/>
    <dbReference type="GeneID" id="33316535"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09645"/>
    <dbReference type="OrthoDB" id="121502at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18545">
        <property type="entry name" value="ABC_6TM_YknV_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03254">
        <property type="entry name" value="ABCC_Glucan_exporter_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000299">
        <property type="entry name" value="ABC transporter ATP-binding protein/permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF1">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B MEMBER 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="134"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="240"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="24"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="338"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ07562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="580" mass="64581" checksum="D019DB66BB9F8200" modified="2017-09-27" version="1">MNESSLSLIVRFVREALSERKTLAIVVVSIIGSALANLASPYLLSVAIDRYIVPGRYEKLGLIAALYLLALAGQWFFMTLQTYYTEVFGQKVLRKLRSRLHEKVLSANLDFFKEKSTGDLVSRIINDTGVVNDVLVSGLLGGLGSLLSLAGIIVAMLILDLRLTLVTLTSVPLMVVVAYYFGGKMRKAYRETRRKIARISSVVEESVAGIETIRAFGRERHVEREFSEASLETIKAYLRVAVYMGLFWPLMNITSLLSVVVVIAYGGYLAYQGSVSIGVVVAFIQYAQRFRGPINNVVSMYDSLQSALAALERIYEVLDDERVEDYNGKTVEKLRGEIEFRDVCFEYEKGRPVLKGINLSIPAGSKVALVGRTGAGKTTIANLIMRFYDPTRGSVLYDGTDGREISRKSLRRRIGYVPQETYLFPGTIMENILIANPKASEEDVVRVCRELGIHDFIMRLPKGYETQAGEAGKLLSVGEKQLISLARAMLKDPDVIILDEALSSVDPKTERLVQDAMLRLMEGRTSIIIAHRLGITRFVDRVVVVEDGEIVEEGPPEALLERKGRFYRLYTSQLSGAEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA21</accession>
    <name>A0A218PA21_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01865</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA21"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01865"/>
    <dbReference type="OrthoDB" id="85230at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="106" mass="12457" checksum="873996489E6F5B62" modified="2017-09-27" version="1">MTAKDWKRRLREEGYIELDGLRIELSLDNTFLDLDYIPRVLVYSEETGRWHVLRNHIPKGKTLEESWDNAVKVLEEIAEGRVRPDLGEPRVEERLVSVLRELEGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA27</accession>
    <name>A0A218PA27_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Orotate phosphoribosyltransferase</fullName>
            <shortName evidence="6">OPRT</shortName>
            <shortName evidence="6">OPRTase</shortName>
            <ecNumber evidence="2 6">2.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">pyrE</name>
        <name evidence="8" type="ORF">A3L08_01500</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:10380"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="2 6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_394335138.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA27"/>
    <dbReference type="GeneID" id="33314905"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01500"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01208">
        <property type="entry name" value="PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023031">
        <property type="entry name" value="OPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004467">
        <property type="entry name" value="Or_phspho_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00336">
        <property type="entry name" value="pyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278">
        <property type="entry name" value="OROTATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278:SF9">
        <property type="entry name" value="URIDINE 5'-MONOPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 6" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="7">
        <location>
            <begin position="34"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <begin position="117"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="183" mass="19848" checksum="1DD2A1DEA2DB30C8" modified="2017-09-27" version="1">MKDELIDAFFSEGAILFGRFVLTSGRESDYYINVKKLSTNPRALRLIARLMAEEAEKAGVEFDRVAGPELGAVPIATALALETGNPLVIVRKKPKGHGTGSQIEGEINPGEKILLVEDVTTTGGSVLRAAEVIEKLGGKIAAISVVVDREEGAEEKIGERYPFLPLVRVSELFERRGLRSAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA41</accession>
    <name>A0A218PA41_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sodium:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_06560</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA41"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06560"/>
    <dbReference type="OrthoDB" id="19265at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050622">
        <property type="entry name" value="CPA3_antiporter_subunitB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007182">
        <property type="entry name" value="MnhB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33932">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33932:SF4">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04039">
        <property type="entry name" value="MnhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="122"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Na+/H+ antiporter MnhB subunit-related protein" evidence="7">
        <location>
            <begin position="91"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="229" mass="24663" checksum="91A1A679A0EBD7F7" modified="2017-09-27" version="1">MLITLSAAYLLQSHVEDIVGVGTLGEFYLENSYLGERSAGSPEVVTSILWDYRGIDTYFETAVLFLAIISAVSVFRSFKGREFRGDGFTEIVKMGVKLVAFTTFVASASVAFHGHITPGGGFQGGSMLAVSALLIIAGFSKKALERNGITKTRALGIRTLGLLIIVCVAVYPLLRGFHFMQNLPVYPAKVGELLVSGSLLPYNLAEFLAVGAGFTIIFLLLANPEEGWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA83</accession>
    <name>A0A218PA83_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_07410</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854915.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA83"/>
    <dbReference type="GeneID" id="33316085"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07410"/>
    <dbReference type="OrthoDB" id="85243at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="111" mass="12952" checksum="5D29FBD48BE7FB60" modified="2017-09-27" version="1">MDDEPPNSWFSRLFKPRERPALLEQKLSREAYEDYKALLMKARPEVKGSKITVRLSDGTVELEEGVLRVKAKNRKTAEKILRNLHHYEQPPSLWPAYGLSYSLKRKRGTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P4W5</accession>
    <name>A0A218P4W5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Prenyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_00010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853091.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P4W5"/>
    <dbReference type="GeneID" id="33314605"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00010"/>
    <dbReference type="OrthoDB" id="86097at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ05829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="427" mass="48075" checksum="FC1D419A1F215897" modified="2017-09-27" version="1">MIVKEILASVDVIPDPYVKSVTYAKIGERLAKARDSNYRTAFLMALETANEIDDPVKMFRALLSVGYSLGRAGLKSSKKIYQGVLEDSRILPPPQRDKIMKTAASYMLTLGEIGEAITYALEIDNSKLRNEVLLEIIRANTRMIGKGQLKAAYRIRKSKLALEYIEEEPYRSRALLELIKAYITLGSYENGISLLRTIGSREWAKQAFKEVSFYLKEKEVLGHYIDSLETIAGEFIQKFGKEFTEELALAFALSGEGVSAVELIRKLENSDEVFVKIALELLERDHDVLPAFIAALDEGEAELVGRVVMNRILERPELGDWEVIKAIGKSTPSEEIWAKIARYYVLVGELEGAMKIGSTLRDSRLRSIVMADVAHHLVKDGDVERAIDAALEVRDPRFSSILVSEILIKALEQELPRRVKQWNGSRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P502</accession>
    <name>A0A218P502_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 11">Thymidine kinase</fullName>
            <ecNumber evidence="2 11">2.7.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">tdk</name>
        <name evidence="14" type="ORF">A3L08_00075</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>thymidine + ATP = dTMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19129"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17748"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.21"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the thymidine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.21" evidence="2 11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P502"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00075"/>
    <dbReference type="OrthoDB" id="372131at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004797">
        <property type="term" value="F:thymidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046104">
        <property type="term" value="P:thymidine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000026">
        <property type="entry name" value="Thymidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00124">
        <property type="entry name" value="Thymidine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001267">
        <property type="entry name" value="Thymidine_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020633">
        <property type="entry name" value="Thymidine_kinase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003296">
        <property type="entry name" value="PRK04296.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11441">
        <property type="entry name" value="THYMIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11441:SF0">
        <property type="entry name" value="THYMIDINE KINASE, CYTOSOLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00265">
        <property type="entry name" value="TK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF035805">
        <property type="entry name" value="TK_cell"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00603">
        <property type="entry name" value="TK_CELLULAR_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="8 11 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 11" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="11 12">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="13"/>
            <end position="20"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="91"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="174"/>
            <end position="177"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR035805-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR035805-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ05840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="193" mass="21647" checksum="8CA7A9C6053CE50D" modified="2017-09-27" version="1">MVHPGGFLEVITGPMFAGKTTELIKRIERQMFAKRKAAFFKPAMDSRYSKDEVVAHNGLRYEAFVIPTDEKGVERIVEITKRDGYEVIGVDEVQFFHISVVEALNRLADEGVYVIASGLNLDFKGDPFPVMRELLVRADNIVYLTAVCTVCGRPATRSQRLIDGKPAPRNSPVILVGGRESYEARCREHHLVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P535</accession>
    <name>A0A218P535_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">4-hydroxybenzoate polyprenyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_00345</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P535"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00345"/>
    <dbReference type="OrthoDB" id="305381at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1780">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050475">
        <property type="entry name" value="Prenyltransferase_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42723">
        <property type="entry name" value="CHLOROPHYLL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42723:SF1">
        <property type="entry name" value="CHLOROPHYLL SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="109"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="212"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ05892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="268" mass="28444" checksum="ABB23B57531F462E" modified="2017-09-27" version="1">MASVSAVIKNLRPFEGRAYVALIGLALLMNLQEAGPEELITALIAGVLFVWYAFSINNCFDVDTDSLNPLKARKNPVASGELTLREGLGLSALLAMAGTGLAFTLNREAFAVYAVMTTLATIYSAPPRLKARPVVDVLSHGLFFGGLPFLYGALVDGSLSQTEIWLAAAITFYSFALELRNHLEDYESDLKAGLRTTPVVIGKGKSEALVDIFSLIAIGIVLYTLTPPMAVVSSLVVARRGLGIKPKTAHRAFDAAMVVALLLAGGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P543</accession>
    <name>A0A218P543_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ferredoxin-NADP reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_00330</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="1">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853152.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P543"/>
    <dbReference type="GeneID" id="33314670"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00330"/>
    <dbReference type="OrthoDB" id="35401at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06219">
        <property type="entry name" value="DHOD_e_trans_like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012165">
        <property type="entry name" value="Cyt_c3_hydrogenase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019480">
        <property type="entry name" value="Dihydroorotate_DH_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050353">
        <property type="entry name" value="PyrK_electron_transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004862">
        <property type="entry name" value="PRK06222.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43513">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE B (NAD(+)), ELECTRON TRANSFER SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43513:SF3">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE B (NAD(+)), ELECTRON TRANSFER SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10418">
        <property type="entry name" value="DHODB_Fe-S_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006816">
        <property type="entry name" value="Cyc3_hyd_g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR006816-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ05889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="291" mass="32301" checksum="58753CAC62ABD47D" modified="2017-09-27" version="1">MPYKILDKKELAMNEVWYKIHAPHVAKKVQPGQFVIVRAFPNGERIPLTPVTWDRDEGWITLITFVRGKTTMRMANELKPGDELLNVAGPLGNPAPMKKFGKILAIGMITGIVEVFPIAKAWQEAGNEVTTLHVSPNPMVILKEEFENAVSRHIVEGFDLQPGWGMNEIAQELVKRAVAKVKELLENEHFDMILTVGPAGGQKAVFNVVKEFGIPMHADLHPIMVDATGMCGACRVTVGGEVKFACIDGPGFDAYKVDWDELIHRSGFYAPMERLALETYLKQLQAQGGEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P560</accession>
    <name>A0A218P560_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_00515</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853186.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P560"/>
    <dbReference type="GeneID" id="33314707"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00515"/>
    <dbReference type="OrthoDB" id="93059at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ05923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="97" mass="11019" checksum="0B54F32E2C6E553D" modified="2017-09-27" version="1">MPCIKPPQPEKVKELKEESFIREGKGKLRVVIESENERLETTISGSLKSVDEIAEMLGVEAKEGKIETVVDGVKFRMKRGKLELEFENGDKMRIEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P588</accession>
    <name>A0A218P588_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_00505</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853184.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P588"/>
    <dbReference type="GeneID" id="33314705"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00505"/>
    <dbReference type="OrthoDB" id="11138at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004451">
        <property type="entry name" value="MJ0586"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00270">
        <property type="entry name" value="multiprotein bridging factor aMBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="2">
        <location>
            <begin position="105"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ05921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="179" mass="20492" checksum="E276A4128AD201A9" modified="2017-09-27" version="1">MGKAKPKYCEICGAPIRGPGHRIRLEGAEVLVCDRCYEKYGGKKPGTFSIMPTGRQPARRTYSQPSRPKPAPKPRTERPLVTEEIVEDYAERVYQAIQRSKKSYQELAQEIGLSMNDLRAIAHGHREPTIKEAKKLERYFKIKLIESGGEELLEKKTIPRDYEPTLGDIANIRIKKRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5A4</accession>
    <name>A0A218P5A4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_00610</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853203.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5A4"/>
    <dbReference type="GeneID" id="33314726"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00610"/>
    <dbReference type="OrthoDB" id="6995at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154">
        <property type="entry name" value="LEUCINE-RESPONSIVE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR AZLB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13404">
        <property type="entry name" value="HTH_AsnC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="5"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ05941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="150" mass="17109" checksum="5751BD49EF9F9719" modified="2017-09-27" version="1">MRTGLDEVDRKILVILQKNSRTPLREISKEVGLAESTVYERIKKLREKGIIKKFTVMLDPESLGFKILAFILIKSKAGMYSHVATELKKYPQIVEIYETTGDYDMLVKIRTSGSDELNEFLDRIGEIQGVEATHTMVVLKAHKEVTELPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5A5</accession>
    <name>A0A218P5A5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleotidyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00715</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853219.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5A5"/>
    <dbReference type="GeneID" id="33314747"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00715"/>
    <dbReference type="OrthoDB" id="9287at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05403">
        <property type="entry name" value="NT_KNTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="18"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="225" mass="25835" checksum="55E7D86633F8B188" modified="2017-09-27" version="1">MIEVVDCIGRNLGRVPGLYSLILYGSLVRGDYLPGTSDVDFFAVLEDGIEPEEVLSRIRPVLEECTKPLKPVEVDIAWEWLSNLRDPLRLGYPYKFLTVYQADFRENHIVVTGEDIVELLPEYRVKEILPGRLEGILKNLERFSGNPKMLHILAGETARLMAFLHGSSLKKDDVLRKLEEIGDEEAVEIYHSYLAGRKMEFNGEFLREFIVSRVRELKGLQDNLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5B6</accession>
    <name>A0A218P5B6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_00755</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853227.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5B6"/>
    <dbReference type="GeneID" id="33314755"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00755"/>
    <dbReference type="OrthoDB" id="85626at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ05965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="154" mass="17887" checksum="5EA94D199067E74A" modified="2017-09-27" version="1">MLGFLKRKKKETYGPLVYLSEPTILYHTHTERAILELLEEKLGSNNFVVPSDYGLKTTDHLIPEAEIFVAIAVIGKFTSLVAREIKIAQEHGKKIYTLDVARKGDEILYIFEEGIPEHIEWLTPEETQKLYAAFRGEDFSGFMKFFFGDRKRQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5C7</accession>
    <name>A0A218P5C7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Magnesium-dependent phosphatase-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_00910</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853255.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5C7"/>
    <dbReference type="GeneID" id="33314786"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00910"/>
    <dbReference type="OrthoDB" id="27736at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010033">
        <property type="entry name" value="HAD_SF_ppase_IIIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010036">
        <property type="entry name" value="MDP_1_eu_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01681">
        <property type="entry name" value="HAD-SF-IIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01685">
        <property type="entry name" value="MDP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17901:SF14">
        <property type="entry name" value="MAGNESIUM-DEPENDENT PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17901">
        <property type="entry name" value="MAGNESIUM-DEPENDENT PHOSPHATASE 1 MDP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12689">
        <property type="entry name" value="Acid_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ05993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="158" mass="18727" checksum="5D15DD20078FD2D2" modified="2017-09-27" version="1">MRLLVLDLDGTLWDHGDASRLTPPYEFHGDYLTDAYGEELRLFPGVREFLEWASWRFILTIASWNVEEKVRPILEGFGLWDYFTFPKIEGHPNKADMIRRTLEELTSIGYTVEDVIYVDDRELHVDEIKMTLPEVDFIHMWVDVKSFEELRELLEKMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5E0</accession>
    <name>A0A218P5E0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Replication factor C large subunit</fullName>
            <shortName evidence="7">RFC large subunit</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">Clamp loader large subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rfcL</name>
        <name evidence="10" type="ORF">A3L08_00975</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heteromultimer composed of small subunits (RfcS) and large subunits (RfcL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the activator 1 small subunits family. RfcL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
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    <dbReference type="GeneID" id="33314799"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00975"/>
    <dbReference type="OrthoDB" id="8658at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01508">
        <property type="entry name" value="RfcL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023935">
        <property type="entry name" value="Rep_factor-C_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003227">
        <property type="entry name" value="PRK04195.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003229">
        <property type="entry name" value="PRK04195.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389:SF6">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
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    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="43"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="427"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="427"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="441"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="477"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="51"/>
            <end position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="505" mass="57718" checksum="808A4E0D281599D6" modified="2017-09-27" version="1">MPREAPWVEKYRPRKLSEIVNQEKAIEQVRAWVEAWLHGNPPKKKALILAGPPGSGKTTTVYALAHEYGFEVIELNASDERTYEKIERYVQAAYTMDILGKHRKLIFLDEADNIEPSGAKEIAKLIDKARNPIIMAANHYWEVPKEIRAKAQIVEYKRLTQRDIIKALIRILKREGITVPKEVLYDIAKHANGDLRAAVNDLQTVVTGGVEDAHEVLAYRDTEKSVFQALAQVFATDNAKKAKIAILGVDMFPHELLQWIDENVPYVYYRPEDIARAYEAISRADIYLGRAQRTGAYSLWKYATDMMTAGVAVAGVKKKGFVRIYPPKTIKLLTESKTERTLRDSVVKKIMKEMHMAKLEALETLNYLKAIFENNPDMAAHFVVYLDLDLKEVEFIAGDKEKAKTIWAKSMNIEKKLKREGELEARAREAGKELEEAKEAAEEEEAEKAEGETEEAGEETEEEINEEELEKAEEEIEPVRKEESEKIDRPKKKGKQATLFDFLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5E5</accession>
    <name>A0A218P5E5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1614 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00935</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5E5"/>
    <dbReference type="GeneID" id="33314791"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00935"/>
    <dbReference type="OrthoDB" id="46118at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011672">
        <property type="entry name" value="DUF1614"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07758">
        <property type="entry name" value="DUF1614"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="234" mass="25175" checksum="14CAC343C274CE8F" modified="2017-09-27" version="1">MNNRRLIIPPVSLPVLVIISVLFLLVFMLFSSIVMAAFGKLGMPPGVAYALFVFALVGSFINIPIAEETSYEPVLRVREVRFLGIPYPVPFFDWAERKVIIAVNVGGALVPLSITLYEILRMLYFGRFGLLFNTVLAVTIAALFSHAVARPVRGLGIAMPVFLPPLMAVLLGWVFGDGNPNVVAYISGTLGVLIGADLMNWNKIKNLGAPMVSIGGAGTFDGIFLAGIIAVLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5F2</accession>
    <name>A0A218P5F2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PhoI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_00895</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853252.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5F2"/>
    <dbReference type="GeneID" id="33314783"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00895"/>
    <dbReference type="OrthoDB" id="98457at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ05990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="290" mass="33213" checksum="AD85863D2C157DA6" modified="2017-09-27" version="1">MEVELERMQTFFPASLEIQEELLKAGFKVPYDKETGRKTPVPVVVSSREERKLRRNRLLKAKDFKSDGKFALVPGERTIMEMELTEKGFLILRPKPLEYHLEEMGFVSVPPRIWGTWASFSIPFSFYETLTDFLSEFRREETNGLYLASRGAGRRIEVYAYKGRTRKDLGIPVFGYALGLHGLTLANEYLREKAEENGVPEERLRYLKLGLRKKKETKAGLKVGVVWENGKPSEITLKLSTTEPRVKIQGLYGELIGKSRGELTRTDDWYVVVHAEDFAGALHKVMTAFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5J9</accession>
    <name>A0A218P5J9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Malate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_01230</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the malic enzymes family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853310.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5J9"/>
    <dbReference type="GeneID" id="33314850"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01230"/>
    <dbReference type="OrthoDB" id="45556at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004470">
        <property type="term" value="F:malic enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05311">
        <property type="entry name" value="NAD_bind_2_malic_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="entry name" value="Malic enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051674">
        <property type="entry name" value="Malate_Decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015884">
        <property type="entry name" value="Malic_enzyme_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045213">
        <property type="entry name" value="Malic_NAD-bd_bact_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
        <property type="entry name" value="Malic_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43237">
        <property type="entry name" value="NADP-DEPENDENT MALIC ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43237:SF4">
        <property type="entry name" value="NADP-DEPENDENT MALIC ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000106">
        <property type="entry name" value="ME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00331">
        <property type="entry name" value="MALIC_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malic enzyme N-terminal" evidence="7">
        <location>
            <begin position="14"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Malic enzyme NAD-binding" evidence="6">
        <location>
            <begin position="158"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="428" mass="46036" checksum="0CF06A4E8B9581BC" modified="2017-09-27" version="1">MRPLDLHRNNFPGNGKIEVIPKIPLTGETLSLAYTPGVAEVSLKIAETPDDAFEYTNRGNTVAVVSDGSRVLGLGDIGPLGALPVMEGKALLFKAFGGVDAFPLVLAEKDPDRFVEVVNAVSPSFGGINLEDIASPKCFYILERLRKELEVPVFHDDQQGTASVVLAALINALKVVGKRLDGISVALFGAGAAGFATLGLLTEAGVKPENVRVVELVNGEPRVLTPDLPLEELFPYRGELLSKTNGEGIEGGPEEALSGADVLISFTRPGPGVIKPEWIKRMADDPVVFPLANPVPEILPEEAKKAGARVVATGRSDYPNQVNNLLGFPAIFRGALDVRASTITDGMIIAASKAMASVIEPSEDEIIPSPFHPDVHPAVARAVAEAAIKEGVARVRVKPEEVEERLRRWRRFYEENVVPLNEKRKLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5N4</accession>
    <name>A0A218P5N4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphoserine phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853350.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5N4"/>
    <dbReference type="GeneID" id="33314894"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01445"/>
    <dbReference type="OrthoDB" id="147108at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042175">
        <property type="term" value="C:nuclear outer membrane-endoplasmic reticulum membrane network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.950">
        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039604">
        <property type="entry name" value="Bfr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055545">
        <property type="entry name" value="DUF7121"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31027:SF2">
        <property type="entry name" value="LEBERCILIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31027">
        <property type="entry name" value="NUCLEAR SEGREGATION PROTEIN BFR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23435">
        <property type="entry name" value="DUF7121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="10"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="159"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="300" mass="36385" checksum="CC5D4AABC5E83743" modified="2017-09-27" version="1">MQTKVDPAEIKRIKREIEALEKERNEIRAKLDELEKELQSWMQKRDEKNKEVQQLRQKGREYKAKRDEINKQIQELKKNREEINAKLDLLYQEILEYRTKRDEYNQLRRLKMPPAKIQEKIEKLEWELQTNPNITPDREKQIVDQIQVLATELEILQQADRFHKKLVEARKKVDQLKKARRNISLEIQKLANQSQQFHEQMIQAFNKADEIKKEADEYHAKVVELRDKIREVRKELRAIEKKIREYDEKHKELIAYRLVARMHAKKDASFEKAVEALEKFKRGEKLTLDELLLLQRYNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Q0</accession>
    <name>A0A218P5Q0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_01560</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853370.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5Q0"/>
    <dbReference type="GeneID" id="33314917"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01560"/>
    <dbReference type="OrthoDB" id="17771at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1190.10">
        <property type="entry name" value="DNA-binding protein Tfx superfamily, archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029291">
        <property type="entry name" value="Tfx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004645">
        <property type="entry name" value="Tfx_DNA-bd_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018384">
        <property type="entry name" value="Tfx_DNA-bd_euryarc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036657">
        <property type="entry name" value="Tfx_DNA-bd_sf_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003055">
        <property type="entry name" value="PRK03975.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003056">
        <property type="entry name" value="PRK03975.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00721">
        <property type="entry name" value="tfx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14601">
        <property type="entry name" value="TFX_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004932">
        <property type="entry name" value="DNA_bind_Tfx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89915">
        <property type="entry name" value="DNA-binding protein Tfx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="4">
        <location>
            <begin position="11"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="146" mass="16787" checksum="D7B2EC2A36A7178D" modified="2017-09-27" version="1">MGKSFLTEQQIKILRLRARGLKQSEIAEMLGTSRANVSILERRALEKIEKARNTLLLWEQINSKISLEVKAGEDIFTVPEKLFKKADELGVKVPYNTAEVIAFLVEHAPIEDRLARRDFTLFLDAKDRLRISECLLEDFDEVGKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Q2</accession>
    <name>A0A218P5Q2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01605</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5Q2"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01605"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="80" mass="9703" checksum="201C2D8DDBA77149" modified="2017-09-27" version="1">MTFITKEVQGLLRQYVKRYAPEPDKPLVSYFTLEKPLIRRVELINQPIRPKHMRKFFSQEWARESLAHFNFCSHTLLINR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5R0</accession>
    <name>A0A218P5R0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF302 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01615</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_157721570.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5R0"/>
    <dbReference type="GeneID" id="33314928"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01615"/>
    <dbReference type="OrthoDB" id="2559at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd14797">
        <property type="entry name" value="DUF302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.70">
        <property type="entry name" value="TT1751-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005180">
        <property type="entry name" value="DUF302"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035923">
        <property type="entry name" value="TT1751-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38342">
        <property type="entry name" value="SLR5037 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38342:SF1">
        <property type="entry name" value="SLR5037 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03625">
        <property type="entry name" value="DUF302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103247">
        <property type="entry name" value="TT1751-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF302" evidence="1">
        <location>
            <begin position="35"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="122" mass="13827" checksum="5AD5BCC016E3D428" modified="2017-09-27" version="1">MIEAKLVFEGTFEEAEAKVKEVLPQTGFGIVWEQDFTAVVKNKLGIDMPKYKTLGLCNAKVFYDLWQRNKGIGMVAPCHLLLYEEDGKVHAKMAVPEEFWDEEVMAEPFNKVLEALKGVGFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5R4</accession>
    <name>A0A218P5R4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ABC transmembrane type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_01690</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853392.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5R4"/>
    <dbReference type="GeneID" id="33314943"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01690"/>
    <dbReference type="OrthoDB" id="44105at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30465">
        <property type="entry name" value="INNER MEMBRANE ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30465:SF0">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN APPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="116"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="154"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="254"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="300"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="117"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="340" mass="38410" checksum="F8953BBBC4266C9B" modified="2017-09-27" version="1">MHNSLGLFRVVVRFTLVLVLVSFLVGTGVKAGLEQQARNELLNYPDYLELYENATRLGMEPSDYYLKYIAPKKHPELAAPTVVVGFNALISSLAPPKDEMWRKIEHRHFAISRREAFLRTFIFLLVSEIFLVLLSVVVAFWALRSQRVAGILSIFSRVISGIPVWWLGVVFILIIIYSALPDDILISPGPGAGLLTILRAMIFPVLTVVLISFWRLAMEFYMLLRREILSPYVSYKRAVGLPENRILRSVFKAISVPALSVWFSHLIEVEGMVIVVDYLFKLGGLGQMIASSFSGARAETFYFIPSLFTFPLLVFIVLNLIISISIEFIKPKLDPRGGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5R6</accession>
    <name>A0A218P5R6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853387.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5R6"/>
    <dbReference type="GeneID" id="33314937"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01660"/>
    <dbReference type="OrthoDB" id="1018at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="1">
        <location>
            <begin position="131"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="285" mass="32332" checksum="5442F4841C39AF4F" modified="2017-09-27" version="1">MDELYFLTSKDARRLLFSGDKVRLNLDLRKTNRTSEITRDGDEFVFPDGTRVEKSVIERIAKDNDTVYFVKNGGVYKAAIAGESGFYKLVPTIPPTIEINGIRMHRTKEVNPLQDTRNKVNAVKPREGETVLDTCMGLGYTAIEASKRGAYVITVEKDPNVLELARINPWSRELFTGGKVQVIQGDSFEVVKRFKPESFDVVIHDPPRFSLAGQLYSEEFYRELFRVLKPGGRLFHYVGNPGKKYRKKDLQKGVMERLRKAGFVGVKRVEEALGVVARKPEREGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5S3</accession>
    <name>A0A218P5S3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01775</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853407.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5S3"/>
    <dbReference type="GeneID" id="33314960"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01775"/>
    <dbReference type="OrthoDB" id="84571at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="115" mass="13029" checksum="ADF19623465B996D" modified="2017-09-27" version="1">MFGYWDALYFVFAFIIGLILAYLLDRWAKRSGMGTRETGPGTKIFISGEDPDRVIPGFEHLEGNYTGKNVMWGLIYALRNFFTALKNEHTGLLTDYASYLMITAAFVMGVLLIWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5U6</accession>
    <name>A0A218P5U6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853394.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5U6"/>
    <dbReference type="GeneID" id="33314945"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01700"/>
    <dbReference type="OrthoDB" id="147058at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="75" mass="8297" checksum="05BB0A7A9248E18B" modified="2017-09-27" version="1">MFGGRFFSYRWDVVSYLIGLVIPVAVMGIFAGLVTINANIEQYGTDSFLQFFLIGFIVHNIILSGFPQQSVECAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Y5</accession>
    <name>A0A218P5Y5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Calcium-gated potassium channel protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_02030</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853455.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5Y5"/>
    <dbReference type="GeneID" id="33315011"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02030"/>
    <dbReference type="OrthoDB" id="43518at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050721">
        <property type="entry name" value="Trk_Ktr_HKT_K-transport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43833">
        <property type="entry name" value="POTASSIUM CHANNEL PROTEIN 2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43833:SF9">
        <property type="entry name" value="POTASSIUM CHANNEL PROTEIN YUGO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0407">Ion channel</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="3">
        <location>
            <begin position="117"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="339" mass="37213" checksum="63A47FB9177CD49A" modified="2017-09-27" version="1">MIPIPIVWRLLRVRIKVRRNRLVQIALAVLLLALGFAILFAHFEGVDFYTAFYWAIITMATIGYGDITPQTGAGRAVAMVAAVAGISTFTALVSVLAEYFISSSLRRMMGMHRVKYSGHYVVIGQGSSIESCVNELLLAISSGDAEPRPIVVVFPSEEERKKVELPEEVEVLVGDPTNQETLERAHVKDASHVILALEDDSKSVFATLMIKRMSSAKVLVEALKAESVELLRQAGADRVILSRSFAGRLLASSIFEPEVVDVIDDLTTALGRYDISVILKRDLWGMSYVEALRKLRSDCGCFLLGYYTDQPVLNPSLDEAIPEGARLIVIKPAVSTGND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Y8</accession>
    <name>A0A218P5Y8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">TIGR00374 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_01960</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0104 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853443.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5Y8"/>
    <dbReference type="GeneID" id="33314997"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01960"/>
    <dbReference type="OrthoDB" id="15513at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022791">
        <property type="entry name" value="L-PG_synthase/AglD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00374">
        <property type="entry name" value="flippase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39087">
        <property type="entry name" value="UPF0104 MEMBRANE PROTEIN MJ1595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39087:SF2">
        <property type="entry name" value="UPF0104 MEMBRANE PROTEIN MJ1595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03706">
        <property type="entry name" value="LPG_synthase_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="226"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="314"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="350" mass="39444" checksum="5967B2DC09742743" modified="2017-09-27" version="1">MNHRKYSLLGVGLLIIFLLLWWAGLEDVLEILRTARPDYFALAVLAYIGSLLAWALRWRVLLKSLDIGVGFKTIVGALFAGVFVNNVTPGARGGGEPVRMYYISKRTGEPYGHVFATVMMDRIMDVIPVVFMLLIATVHVYRLGSFTLTLTIFLLDVFFAFVTLATVGILLSERKTKGILYWFYRRFKRLMPGKAIKYEEKFVHAVEVSVPQFQENFKVLMRHKRAFVLSLFWSFVSWTLILLRSYFIFISINSPIKLVDVMVVQMIGIVVGMFAVVPGGAGLIEAINSGVYVLLGINKEIAVTATILERLISYWAPTFIGAGILTHFGIKIEEKRASDNDINEEAQEKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P621</accession>
    <name>A0A218P621_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="16">ATP pyrophosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">A3L08_02290</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853502.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P621"/>
    <dbReference type="GeneID" id="33315063"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02290"/>
    <dbReference type="OrthoDB" id="33422at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002144">
        <property type="term" value="C:cytosolic tRNA wobble base thiouridylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01713">
        <property type="entry name" value="CTU1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000174">
        <property type="entry name" value="ATPase, PP-loop superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056369">
        <property type="entry name" value="CTU1-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000541">
        <property type="entry name" value="Ncs6/Tuc1/Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054306">
        <property type="entry name" value="TtuA-like_LIM_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00269">
        <property type="entry name" value="TIGR00269 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807">
        <property type="entry name" value="ATPASES OF THE PP SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807:SF12">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22082">
        <property type="entry name" value="TtuA_LIM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="2-thiouridine synthetase TtuA-like N-terminal LIM" evidence="15">
        <location>
            <begin position="3"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal" evidence="14">
        <location>
            <begin position="51"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="4"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="54"/>
            <end position="56"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR004976-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR004976-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF22082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ASJ06239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="324" mass="37404" checksum="57E0416C47D2A179" modified="2017-09-27" version="1">MPKCSKCGRPAVYHARYTGMYYCHKHFNEMVEKKFKETVKKYRLIEKGERIAVGVSGGKDSVVLMHLLAKLREKFPFELVAITIDEGIAGYRPPSVEIAKRNAEKLGIEHRSYSFKEYIGFTLDETVEIMGSFEKGERVGACSYCGVWRRWLLNYAAKDVGADKLAVGHNLDDEVQMFVMNILRGDIARLGRTGPYYEEIHPELVPRIKPLREIPEKEIVLYAVLNNIEVDLSECPYAVEAFRAEIRDWLNEMEEKHPGTKYQILRSYDKLFPLIAKTYTKRTSELNRCKICGQPTTGEICKACQFRLQVERKAREKGLTFRVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P651</accession>
    <name>A0A218P651_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 5 6">Adenylyl-sulfate kinase</fullName>
            <ecNumber evidence="1 5 6">2.7.1.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">APS kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">ATP adenosine-5'-phosphosulfate 3'-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Adenosine-5'-phosphosulfate kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cysC</name>
        <name evidence="8" type="ORF">A3L08_02340</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Catalyzes the synthesis of activated sulfate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>adenosine 5'-phosphosulfate + ATP = 3'-phosphoadenylyl sulfate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24152"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5 6">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the APS kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.25" evidence="1 5 6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853511.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P651"/>
    <dbReference type="GeneID" id="33315073"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02340"/>
    <dbReference type="OrthoDB" id="28808at2157"/>
    <dbReference type="UniPathway" id="UPA00140">
        <property type="reaction ID" value="UER00205"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004020">
        <property type="term" value="F:adenylylsulfate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004781">
        <property type="term" value="F:sulfate adenylyltransferase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070814">
        <property type="term" value="P:hydrogen sulfide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010134">
        <property type="term" value="P:sulfate assimilation via adenylyl sulfate reduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019379">
        <property type="term" value="P:sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02027">
        <property type="entry name" value="APSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00065">
        <property type="entry name" value="Adenylyl_sulf_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002891">
        <property type="entry name" value="APS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059117">
        <property type="entry name" value="APS_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050512">
        <property type="entry name" value="Sulf_AdTrans/APS_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00455">
        <property type="entry name" value="apsK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004041">
        <property type="entry name" value="PRK05541.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42700">
        <property type="entry name" value="SULFATE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42700:SF1">
        <property type="entry name" value="SULFATE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01583">
        <property type="entry name" value="APS_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="APS kinase" evidence="7">
        <location>
            <begin position="9"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="5">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="17"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="181" mass="20461" checksum="03C5C82A075EDB3F" modified="2017-09-27" version="1">MTGLKNLEKGFTIWLTGPSGAGKTTLAVKLAKRLREMGYRVEILDGDTIRKTLYPNLGFSKEAREMHNRVVIHMAKLLSRNGVIAIVSLISPYRAVREYARKEIVDFIEVYVYAPLEVRIQRDPKGLYAKALRGEIKGLTGYDGVYEEPENPEVRVDSSKMTPEEEVEAVITKAREMGYLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P678</accession>
    <name>A0A218P678_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">4-alpha-glucanotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_02405</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 57 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853522.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P678"/>
    <dbReference type="GeneID" id="33315086"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02405"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10793">
        <property type="entry name" value="GH57N_TLGT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015179">
        <property type="entry name" value="A-amylase/a-glucTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015178">
        <property type="entry name" value="A-amylase/a-glucTrfase_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052046">
        <property type="entry name" value="GH57_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028995">
        <property type="entry name" value="Glyco_hydro_57/38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004300">
        <property type="entry name" value="Glyco_hydro_57_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36306:SF1">
        <property type="entry name" value="ALPHA-AMYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36306">
        <property type="entry name" value="ALPHA-AMYLASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09094">
        <property type="entry name" value="AmyA-A_glucT_m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09095">
        <property type="entry name" value="AmyA-gluTrfs_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03065">
        <property type="entry name" value="Glyco_hydro_57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88688">
        <property type="entry name" value="Families 57/38 glycoside transferase middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycoside hydrolase family 57 N-terminal" evidence="3">
        <location>
            <begin position="5"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-amylase/4-alpha-glucanotransferase central" evidence="4">
        <location>
            <begin position="309"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-amylase/4-alpha-glucanotransferase C-terminal" evidence="5">
        <location>
            <begin position="390"/>
            <end position="648"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF09095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="653" mass="76274" checksum="11CD8891D1539DD9" modified="2017-09-27" version="1">MVNFIFGIHNHQPLGNFGWVFESAYDRSYSPFMETLEEYPNMKVAAHYSGPLLEWLSDNRPEHIDLLRSLVKKGQLEIVVAGFYEPVLAAIPKEDRIEQINMLKDFAKKIGYEAKGVWLTERVWQPELVKSLRAAGIEYVIVDDYHFMSAGLAKEELYWPYYTEDGGEVIAVFPIDEKLRYLIPFRPVEKTLEYLHSLDDGDESKVAVFHDDGEKFGVWPGTYEWVYEKGWLREFFDRVSSDERINLMLYSEYLSKFKPRGLVYLPIASYFEMSEWSLPAKQAKLFVEFIEELKRENKFEKYRVFVRGGIWKNFFFKYPESNYMHKRMLMVSKAVRDNPEARQFVLKAQCNDAYWHGVFGGVYLPHLRRALWENIIRANSFVSTGNFVRDIDFDGRDEVFIEDENFYAVFKPVYGGALFEFSSKRKAVNYNDVIARRWEHYHEVPEAATPEEDDGEGVASIHELGKQIPEEIRRELAYDSHLRAILQDHFIEPETTLDEYRLNRYLELGDFISGAYDFSLFENGLTLERDGTVSGKPAKVEKTFHLTDDGFVVDYTVRSDAKALFGVELNLAVHSVMEEPAEFEAESFEVDDPYGIGKVRIELDKKARVWKYPIKTLSQSESGWDFIQQGVSYTVLFPVDGELRFRLRFKELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P680</accession>
    <name>A0A218P680_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nif3-like dinuclear metal center hexameric protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_02495</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP cyclohydrolase I type 2/NIF3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853539.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P680"/>
    <dbReference type="GeneID" id="33315104"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02495"/>
    <dbReference type="OrthoDB" id="85198at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1390.30:FF:000001">
        <property type="entry name" value="GTP cyclohydrolase 1 type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1390.30">
        <property type="entry name" value="NIF3 (NGG1p interacting factor 3)-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002678">
        <property type="entry name" value="DUF34/NIF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036069">
        <property type="entry name" value="DUF34/NIF3_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00486">
        <property type="entry name" value="YbgI_SA1388"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799:SF14">
        <property type="entry name" value="GTP CYCLOHYDROLASE 1 TYPE 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799">
        <property type="entry name" value="NGG1 INTERACTING FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01784">
        <property type="entry name" value="DUF34_NIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102705">
        <property type="entry name" value="NIF3 (NGG1p interacting factor 3)-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR602678-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="250" mass="27586" checksum="14652FEA0D3DB09D" modified="2017-09-27" version="1">MNRDELVAFLDEYLQISAYPDKSSNGLQVEGKEDVERVAFAVDTTLKTIERATKAKADMLIVHHGMIWGGLNYITGVHYKRLKALFESGLNLYVAHLPLDAHPEVGNNVELLKLLGLEPKGSFGEYRGLSIGYWGEFDEPQPIEKVAQVIAEKLDTTVKTYEFGEREIKAVGAVSGAGAFALEEAWRKRIDLLITGEFGHADYLTAIDLPQSVLVAGHYKTETLGVKALMGLLKGRFGLDAFFIDEPTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P681</accession>
    <name>A0A218P681_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CopG family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_014788819.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P681"/>
    <dbReference type="GeneID" id="74506285"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02360"/>
    <dbReference type="OrthoDB" id="359411at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22231">
        <property type="entry name" value="RHH_NikR_HicB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="75" mass="8751" checksum="B0CDBA3A748752A9" modified="2017-09-27" version="1">MAEEKKYTTVSIPKPLYDKIKARIEGTGFTSVSDYVTYVLREVLASLEEEEKEEVFTEEEEEKVKERLRALGYLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P696</accession>
    <name>A0A218P696_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">A-type ATP synthase subunit E</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">atpE</name>
        <name evidence="9" type="ORF">A3L08_02585</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the V-ATPase E subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853556.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P696"/>
    <dbReference type="GeneID" id="33315123"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02585"/>
    <dbReference type="OrthoDB" id="4691at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.30">
        <property type="entry name" value="ATP synthase, E subunit, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.620">
        <property type="entry name" value="F1F0 ATP synthase subunit B, membrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00311">
        <property type="entry name" value="ATP_synth_E_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028987">
        <property type="entry name" value="ATP_synth_B-like_membr_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038495">
        <property type="entry name" value="ATPase_E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002842">
        <property type="entry name" value="ATPase_V1_Esu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003049">
        <property type="entry name" value="PRK03963.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45715">
        <property type="entry name" value="ATPASE H+-TRANSPORTING V1 SUBUNIT E1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01991">
        <property type="entry name" value="vATP-synt_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81573">
        <property type="entry name" value="F1F0 ATP synthase subunit B, membrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160527">
        <property type="entry name" value="V-type ATPase subunit E-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 7" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="203" mass="23309" checksum="DFB4E70A88B0AA9D" modified="2017-09-27" version="1">MEGAELIIQEINREAERKIQYILSEAREEAEKIKAEARKRAEAKAEWILRKARTQAEIEKQRIIANARLEVRKKRLQIQEELIREVIKALRERLSELPEEEYFPMLVDLAASAVKELGAESVVVRSNERTLKLLSERLDEFRKELAEKLDGEVEISLGEPINTIGGLVVETLDGSVRVDNTFEARIERFEGELRAEIAKALFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6A6</accession>
    <name>A0A218P6A6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_02750</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853588.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6A6"/>
    <dbReference type="GeneID" id="33315156"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02750"/>
    <dbReference type="OrthoDB" id="84637at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043960">
        <property type="entry name" value="DUF5748"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19022">
        <property type="entry name" value="DUF5748"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="102" mass="12101" checksum="B5BB4E2DA58D425F" modified="2017-09-27" version="1">MHFEVVKEFLEEIGEDWVEVDGEIHLEPEVFYEVWKYVGQPDLETYTVEDEVVEPGSYDPPEMKYTEMKKVKVKKAYFTTLDGKRIVTDYAELQRIMKEKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6C0</accession>
    <name>A0A218P6C0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glycogen synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_02835</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853605.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6C0"/>
    <dbReference type="GeneID" id="33315173"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02835"/>
    <dbReference type="OrthoDB" id="132546at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013534">
        <property type="entry name" value="Starch_synth_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45825:SF11">
        <property type="entry name" value="ALPHA AMYLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45825">
        <property type="entry name" value="GRANULE-BOUND STARCH SYNTHASE 1, CHLOROPLASTIC/AMYLOPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08323">
        <property type="entry name" value="Glyco_transf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Starch synthase catalytic" evidence="4">
        <location>
            <begin position="2"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="3">
        <location>
            <begin position="252"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="450" mass="50452" checksum="5FD4CE069FAF66DF" modified="2017-09-27" version="1">MRILILGFEYLPVKVGGLAEAITSIAEGLAGLGHEVVVFTPDHGKGLGEPVKRFKVISFGEEVDIEVRKRVQNGVTVYSLAGGLLSEPDVYGPGWDGLLKKTVLFGKASAGLMNSLIGEFKPDVVHAHDWHTVFALGLLKKYFGIRSVFTIHRLNKAKVPAGYFHEANLSELAPYPDIDPEHTAAYIADVVTTVSRSYLWEEWDFFRHFEGKVTHVFNGIDCTFWNEGLLKNADLPREERRKLILERFGLSDGKAFMFIGRFDRAQKGVDTLLKAIEILSTDPAFNDMRFIIIGKGDPELERWAKAVENRFPGNVRVITELLSRETVRELYGSVDFVLIPSYFEPFGLVQLEAMCLGAVPIGSAVGGIKDTIIDLDEDPENATGILVPPRDAFALAKAMIIAKNLDEETLNRLRENGRRRGREDFTWKKACERYVKAYLNEVDRAMSFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6G6</accession>
    <name>A0A218P6G6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase RgtA/B/C/D-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853647.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6G6"/>
    <dbReference type="GeneID" id="33315221"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03075"/>
    <dbReference type="OrthoDB" id="137309at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="375"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="404"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="436"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="591" mass="68517" checksum="86BF936568A7E383" modified="2017-09-27" version="1">MTKNMHKVLNFIDGIIFVFTLSVYLYTMTKIQPASGYEISIYNGISRVLYIVIILYITGVVNIYYQKGVNKILVMLLLTVVTITLILLPELKGYAFFGRHDSMTHAGLVRDILITSRVYEQYNIYPILHIYTAMLLKIQGIDSIFTASREVIVMYFGTFIVFAIMILKGLHPNLKTHQILFPIILFTIPLLTEPIILFRPSSNLFLVIPPILVYLFIIKNWSMRAIKYLVIPFYLLLPFAHFEFVLFATTLVISIIVTKYLYSNNKEDKSNLVLSHIGTLTVFIVLFNVFYVLNSTRGMKYANRIYYVISGITNKSPAEYYGQFVTNRQIPLIEILKNALIPNVAYIVFLGTTGLVLFIVWKYKDEFLFNDTRLFSIIIISSTQIIIFVILAIVSIWVDIVIKYYRFLKYPSLFTFMLLGILMGSNKIMHKKITKIVILSITILIVFGTVVSVKTFGVTPAQITRYNPQVTYSEISGSAWLVQRGACYPISSRMYIPYRFFSFIYGQYRATHICYNKLPRLYPDHFNYNSKTSLQTTYLILTRFEETFYLKIPEVKKLHKFNEIDYMRLQADNYFQKVYVAPGMKIFLGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6L7</accession>
    <name>A0A218P6L7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Phosphoribosylamine--glycine ligase</fullName>
            <ecNumber evidence="4 14">6.3.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">GARS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12 14">Glycinamide ribonucleotide synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13 14">Phosphoribosylglycinamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">purD</name>
        <name evidence="17" type="ORF">A3L08_03255</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>5-phospho-beta-D-ribosylamine + glycine + ATP = N(1)-(5-phospho-beta-D-ribosyl)glycinamide + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17453"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11 14">Belongs to the GARS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.13" evidence="4 14"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853675.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6L7"/>
    <dbReference type="GeneID" id="33315257"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03255"/>
    <dbReference type="OrthoDB" id="146558at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00125"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.10">
        <property type="entry name" value="Phosphoribosylglycinamide synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00138">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020561">
        <property type="entry name" value="PRibGlycinamid_synth_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000115">
        <property type="entry name" value="PRibGlycinamide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020560">
        <property type="entry name" value="PRibGlycinamide_synth_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037123">
        <property type="entry name" value="PRibGlycinamide_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020559">
        <property type="entry name" value="PRibGlycinamide_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020562">
        <property type="entry name" value="PRibGlycinamide_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00877">
        <property type="entry name" value="purD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43472">
        <property type="entry name" value="PHOSPHORIBOSYLAMINE--GLYCINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43472:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLAMINE--GLYCINE LIGASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01071">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02843">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02844">
        <property type="entry name" value="GARS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01209">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01210">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00184">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 14 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 14" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="16">
        <location>
            <begin position="108"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ASJ06417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="438" mass="47997" checksum="377C09EC1934E601" modified="2017-09-27" version="1">MKILLVGGGGRENAIGEALVGSGAELYVVSKHRNPGLARLAKGYGLAKETDVGKVLVYAEKFGVEVAFIGPEAPLGTGIVDILEENGIPAVGPSKEAAKLETDKAFARQFMERHKIPGRKAFKVFDDVSEMRSWIDDFGKPVVVKPLGLTGGKGVKVVGYQLRDNDEAKAYAEELIKRDGKVLVEERTDGVEFTFQVFSDGKHVIPMPLAQDYPHAYENDEGPITGGMGSYSCSNGLLPFVTKEDYERAFETLKATIEAMRKEGTPYKGILYGQFMLSKDGPVIIEYNARFGDPEAMNVLPLLKTPLTEVAEGIVDGNLRKAEFEEKATVVKYLAPKGYPVNPIRGVNVEVDERAVEEAGARLYYASIDENFKLLGSRAIAVVGIAETLEEAERIAESAVPHIKGELFYRRDVGTRGSVEKRIRLMRELGKEFEPNSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6M0</accession>
    <name>A0A218P6M0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GTP-dependent dephospho-CoA kinase</fullName>
            <ecNumber evidence="6">2.7.1.237</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Dephospho-coenzyme A kinase</fullName>
            <shortName evidence="6">DPCK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_03370</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the GTP-dependent phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A (CoA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3'-dephospho-CoA + GTP = GDP + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61156"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="2.7.1.237"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GTP-dependent DPCK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.237" evidence="6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6M0"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03370"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00590">
        <property type="entry name" value="Dephospho_CoA_kinase_GTP_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054930">
        <property type="entry name" value="deph_CoA_kin_Thcocales"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007164">
        <property type="entry name" value="GTP-dep_dephospho-CoA_kin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041125">
        <property type="entry name" value="deph_CoA_kin_Thcocales"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002248">
        <property type="entry name" value="PRK01160.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40732:SF1">
        <property type="entry name" value="GTP-DEPENDENT DEPHOSPHO-COA KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40732">
        <property type="entry name" value="UPF0218 PROTEIN TK1697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04019">
        <property type="entry name" value="DUF359"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006533">
        <property type="entry name" value="UCP006533"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="178" mass="19791" checksum="0779596515D9FD6E" modified="2017-09-27" version="1">MMLYFKLTPELRRELKHPLGRLVRGEIPEPYLRVRGELEGARHVVTVGDVVTENVLRLGIQPSVAIYDHKTKRHEYNPGIETDAVVMTVQNPAGTVTKALLNAIRKGFGLAERGRRVYIKVNGEEDLAAIPAVLYAPYGSLVLYGQPDEGVVLIKVTPECKLKCGKLMSKMEVVRDGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Q1</accession>
    <name>A0A218P6Q1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SAM-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853712.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Q1"/>
    <dbReference type="GeneID" id="33315299"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03470"/>
    <dbReference type="OrthoDB" id="84950at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="1">
        <location>
            <begin position="44"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="266" mass="29943" checksum="149A34DCF364175A" modified="2017-09-27" version="1">MSLEELYRHINWRMNPGDGRARERFGELLKFFESLEWLPRNGRVLDLCAGTGIAGVALAKATGAKLLTVLDARSDDLELAREWLEVAGINPELRTVQGDAREAAKLAGEHDVAVLWGYTMPHFDPFDAVKLFANVALVLSDDGVFMIEDMDRVYWILYRAGYREFLIEGRKGDHTIASMHEGYDILRGTFKRGYYLLPGFRKVSTVDFHYWDIATQLAIGSVFFREYELIGREEHGVGGVGKILVFKKPDKKVAELKVSSAPAACP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Q9</accession>
    <name>A0A218P6Q9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Fmu (Sun) domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_03530</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853722.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Q9"/>
    <dbReference type="GeneID" id="33315311"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03530"/>
    <dbReference type="OrthoDB" id="14725at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016428">
        <property type="term" value="F:tRNA (cytidine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000022">
        <property type="entry name" value="Ribosomal RNA small subunit methyltransferase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.730">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.940.10">
        <property type="entry name" value="NusB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011023">
        <property type="entry name" value="Nop2p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035926">
        <property type="entry name" value="NusB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054728">
        <property type="entry name" value="RsmB-like_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00446">
        <property type="entry name" value="nop2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF74">
        <property type="entry name" value="TRNA (CYTOSINE(48)-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22458">
        <property type="entry name" value="RsmF-B_ferredox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6 8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="9">
        <location>
            <begin position="170"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="381"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="258"/>
            <end position="264"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="442" mass="50679" checksum="B6397C318CDC771E" modified="2017-09-27" version="1">MGKLKLSDRQLYALIESVKLGEEVKPSQQAKRKAFAKYKIEGWENSKLTGIFYSIQRRLGLIDEIIEELVGVSPLILDPWLRAALRVAVEVAVFRDPNEKTIQHLRGLAQFLSKRTHPYVGYYYYDILPRVLEYMPVIDSEEKRLKWDYLFPEWFIRRVRNLLGDEAEELLKALNETLPTSIRVNRLKVSVEDVENYLRKKNVRFERSERVDTAIRILDPFNPEWLFNKGFAVAQEEAAAVASLVLSPKPGETVVDLAAAPGGKTSHMAELMNNEGKIYAFDIDKARVKRMNEVLRRTGVEIAETIKADGRKAPEILGEGVADRVMLDAPCTSDGTIAKNPELRWRLREKNISKVVALQKELIESAWKLLKPGGRMLYSTCSMLPEENEEVVKWFLKRHENAELIPLNGPYDEGFLPGTMRAWPHRHGTIGFFYALIEKKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6V4</accession>
    <name>A0A218P6V4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thiamine-binding protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_03695</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0045 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853749.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6V4"/>
    <dbReference type="GeneID" id="33315344"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03695"/>
    <dbReference type="OrthoDB" id="10763at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029756">
        <property type="entry name" value="MTH1187/YkoF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002767">
        <property type="entry name" value="Thiamine_BP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051614">
        <property type="entry name" value="UPF0045_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00106">
        <property type="entry name" value="MTH1187 family thiamine-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33777">
        <property type="entry name" value="UPF0045 PROTEIN ECM15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33777:SF1">
        <property type="entry name" value="UPF0045 PROTEIN ECM15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01910">
        <property type="entry name" value="Thiamine_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89957">
        <property type="entry name" value="MTH1187/YkoF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine-binding protein" evidence="2">
        <location>
            <begin position="4"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="100" mass="11380" checksum="4C2294B892057F27" modified="2017-09-27" version="1">MVIVEFVVVPLGERSLSRYVAEVVKLLESKGVKYQLTPMSTIIEVPTVKDAFAVIEEAHELMFKLGAERVSTTIRIDDRRDKERKMEDKVKSVMEKVRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6V9</accession>
    <name>A0A218P6V9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exosome complex component Rrp42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rrp42</name>
        <name evidence="7" type="ORF">A3L08_03865</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Non-catalytic component of the exosome, which is a complex involved in RNA degradation. Contributes to the structuring of the Rrp41 active site.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the archaeal exosome complex. Forms a hexameric ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers. The hexameric ring associates with a trimer of Rrp4 and/or Csl4 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RNase PH family. Rrp42 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853783.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6V9"/>
    <dbReference type="GeneID" id="33315378"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03865"/>
    <dbReference type="OrthoDB" id="30932at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11365">
        <property type="entry name" value="RNase_PH_archRRP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000017">
        <property type="entry name" value="Exosome complex component Rrp42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00622">
        <property type="entry name" value="Exosome_Rrp42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050590">
        <property type="entry name" value="Exosome_comp_Rrp42_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020869">
        <property type="entry name" value="Rrp42_archaea"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003282">
        <property type="entry name" value="PRK04282.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097:SF8">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="5">
        <location>
            <begin position="30"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="6">
        <location>
            <begin position="189"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="269" mass="29550" checksum="74025284F9BBFCC2" modified="2017-09-27" version="1">MEVMAGIMRDRILELLKDGRRIDGRGLEDYRDLEVKVNVIEKAEGSAWVRLGDTQVLVGIKVDMGEPFPDLPDRGVITTNVELVPLASPSFEPGPPDENAIELARVVDRGIRESQAVELEKLVIVPGKLVRVVFIDVHVLDHGGNLLDATGIGAIAALLSTKMPKVRYNEETDEVEVLDEYEPLPVRRLPIPVTFAKIGSNLLVDPSLDEEMVMDGRITITTDENEMISSVQKGEGGSFKLEEVMYAVDTAIKKAAELREKVLEAVKAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P720</accession>
    <name>A0A218P720_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein eL19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rpl19e</name>
        <name evidence="11" type="ORF">A3L08_04140</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the eukaryotic ribosomal protein eL19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853832.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P720"/>
    <dbReference type="GeneID" id="33315432"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04140"/>
    <dbReference type="OrthoDB" id="11624at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00481">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.240:FF:000003">
        <property type="entry name" value="50S ribosomal protein L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1650.10:FF:000001">
        <property type="entry name" value="Ribosomal protein L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01475">
        <property type="entry name" value="Ribosomal_eL19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035970">
        <property type="entry name" value="60S_ribosomal_eL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039547">
        <property type="entry name" value="Ribosomal_eL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023638">
        <property type="entry name" value="Ribosomal_eL19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000196">
        <property type="entry name" value="Ribosomal_eL19_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015972">
        <property type="entry name" value="Ribosomal_eL19_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057259">
        <property type="entry name" value="Ribosomal_L19e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057260">
        <property type="entry name" value="Ribosomal_L19e_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006343">
        <property type="entry name" value="PRK08570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01280">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25476">
        <property type="entry name" value="Ribosomal_L19e_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01416">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48140">
        <property type="entry name" value="Ribosomal protein L19 (L19e)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00526">
        <property type="entry name" value="RIBOSOMAL_L19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL19" evidence="10">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="54"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="148" mass="17442" checksum="E9FF2BF0AAEB20CF" modified="2017-09-27" version="1">MLKMQRRIAADLLKCGENRVWIDPERIDDVAAAITREDVKRLINDGVIKKKPIKGQSRARARAFQEARKKGRHRGPGSKKGKKTARMGKKERWMMTIRALRKELRKLKAEGKLDEHTYRRLYIRAKGGQFKNKRQLYMFMQEHGILKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P764</accession>
    <name>A0A218P764_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Leucine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Leucyl-tRNA synthetase</fullName>
            <shortName evidence="8">LeuRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 12" type="primary">leuS</name>
        <name evidence="12" type="ORF">A3L08_04440</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853879.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P764"/>
    <dbReference type="GeneID" id="33315492"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04440"/>
    <dbReference type="OrthoDB" id="23906at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07959">
        <property type="entry name" value="Anticodon_Ia_Leu_AEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00812">
        <property type="entry name" value="LeuRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000051">
        <property type="entry name" value="Leucine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.740.10:FF:000024">
        <property type="entry name" value="Leucine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.20">
        <property type="entry name" value="Class I aminoacyl-tRNA synthetases (RS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.720">
        <property type="entry name" value="leucyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00049_A">
        <property type="entry name" value="Leu_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020791">
        <property type="entry name" value="Leu-tRNA-lgase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004493">
        <property type="entry name" value="Leu-tRNA-synth_Ia_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00395">
        <property type="entry name" value="leuS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008957">
        <property type="entry name" value="PRK12300.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794:SF1">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="10">
        <location>
            <begin position="12"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="11">
        <location>
            <begin position="726"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="650"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="653"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="967" mass="113735" checksum="A66D1B584C461ADB" modified="2017-09-27" version="1">MAELDFKAIEEKWQRKWMEERVFEPDRNAKPKEKKFYITVAFPYLSGHLHVGHARTYTIPDVIARFKRMQGYNVLFPMGWHITGAPIVGIAERIKNRDPKTIHVYRDVYKVPEEILWKFEDPKEIVKYFMKAAKETFIRAGFSVDWTREFHTTSLFPPFSKFIEWQFWTLKDMGLVVKGAHRVRWDPVVGTPLGDHDIMEGEDVQILDYVIIKFILEEDGEEIYLPAATLRPETVYGVTNMWLNPEATYVKAKVRRNGKEETWIVSKEAAYKLSFQDREIEVIEEFKGERLIGKYVKNPVTGDEIIILPAEFVDPNNATGVVMSVPAHAPFDHIALEDLKKETEILLKYDIDPRVVEEISYISLIKLEGYGEFPAVEEAERLGVKSQKDVEKLEEATKNIYKAEYHKGVFKIEPYAGKPVQEVKDLIAKELQEKGTAEIMYEFAEKPVISRFGNQAVIKIIHDQWFIDYGNPEWKEKAREALANMTIYPESRRAQFEAVIDWLDKKACARKVGLGTPLPWDPDWVIESLSDSTIYMAYYTISRHMNRLRDEGKLDPEKLDREFFDYIFREDFDEEKEKKLAEKTGIPAEIIHEMKEEFEYWYPLDWRCSAKDLIPNHLTFFIFNHTAVFRKEHWPRGIAVNGFGTLEGQKMSKSKGNVLNFIDAIEENGADVVRLYIMGLAEHDSDFDWRRKEVGKLRRQVERFYELISDFSTYEAKEDVELKDIDRWMLHRLNKAIEETTKALEEFRTRTAVQWAFYSVLNDLRWYLRRTEGRDDEAKRYVLRKLAEVWVRLMAPFTPHIAEELWEKLGGEGFVSLAKWPEPVEEWWNETVEAEEQFVQSVIEDIKEIIRVAKLEDAKRVYIYTAPEWKWRVVEVVAEKRDFKSAMAELMKDPEMRKHGKEVSKLIQRLIKERAFEVKRIDEEKALREAKDFMEKELGLEIIINAEEDKGGKKRQAMPLKPAVFVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P772</accession>
    <name>A0A218P772_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Archaeal Rqc2 homolog aRqcH</fullName>
            <shortName evidence="2">aRqcH</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rqcH</name>
        <name evidence="4" type="ORF">A3L08_04500</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Probably part of the ribosome quality control system (RQC). May mediate the addition of alanine residues (Ala tailing) to incompletely synthesized nascent chains from stalled ribosomes, leading to their degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Associates with stalled 50S ribosomal subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NEMF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853890.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P772"/>
    <dbReference type="GeneID" id="33315504"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04500"/>
    <dbReference type="OrthoDB" id="10943at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.310.10:FF:000003">
        <property type="entry name" value="Zinc knuckle domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.310.10">
        <property type="entry name" value="ibrinogen binding protein from staphylococcus aureus domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00844_A">
        <property type="entry name" value="RqcH_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051608">
        <property type="entry name" value="RQC_Subunit_NEMF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043681">
        <property type="entry name" value="RqcH_archaeal"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041120">
        <property type="entry name" value="RqcH_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239">
        <property type="entry name" value="NUCLEAR EXPORT MEDIATOR FACTOR NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239:SF6">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05833">
        <property type="entry name" value="NFACT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="2" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="NFACT RNA-binding" evidence="3">
        <location>
            <begin position="452"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="408"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="650" mass="75275" checksum="1910B30E194C32DE" modified="2017-09-27" version="1">MKEEMSSVDIRYIVRELQWLVGSRVDKVYHDGDEIRIKLRTKEGRADLILQAGKRFHLTSYVKEAPKSPSSFTMLLRKHLSGGFIDAIEQHGFDRIVKVKIGDYTIIGELFRRGNIILVDSENKIVAALRYEEYKDRRIMPKAEYQYPPARENPLEVSKERFLELMREEENLELVRALARKLNMGGMYAEEISIRAGFEKTAPVKELSDDDLLKVYDAMLRTFNDEPRPNIVFKDGNMHDVVPIELRIYDGFEKKYFNTFSEALDEYFGKITVEKAKVEQTKKLEAKKRQLLMTLRKQEEMLKGFEESAKANQEIGDLIYANYALVERLLEEFRKATETLGWDEFKRRIEEGKKAGNKVALMVKGIDPKEKAVTIELEGKKVRLYLNRSIGENAELYYENAKKFRHKYEGALKAYEDTKRKLDEVEKLIEEEMKKELNVKKIERRKKKWFEKFRWFISSEGFLVLAGKDASTNEILIKRHMTDNDLYCHADVYGAPHVVIKDGQKAGEKTVFEACQFAVSMSKAWSRGVYSEDAYWAYPNQVTKQTPSGEYLGKGAFMVYGKRNWLHGLPLKLAVGVINYEGEDYVICAPVDAIKAHTDKYIVIRPGSLKKSELVKRIKHILEKWGYKVREEDIMAVLPPGNGDVVEVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P783</accession>
    <name>A0A218P783_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Peptidase M20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_04570</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="2">The Mn(2+) ion enhances activity.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853901.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P783"/>
    <dbReference type="GeneID" id="33315518"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04570"/>
    <dbReference type="OrthoDB" id="247417at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.360:FF:000001">
        <property type="entry name" value="N-acetyldiaminopimelate deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017439">
        <property type="entry name" value="Amidohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01891">
        <property type="entry name" value="amidohydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014:SF63">
        <property type="entry name" value="METALLOPEPTIDASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G09600)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014">
        <property type="entry name" value="PEPTIDASE M20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005962">
        <property type="entry name" value="Pept_M20D_amidohydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="3">
        <location>
            <begin position="187"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR005962-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="383" mass="42083" checksum="5BD3917F9EBB6B74" modified="2017-09-27" version="1">MSLDPVSEAKKIEKEIIAWRRDFHMHPELGYEEERTSKIVEEHLRKWGYSIKRVGTGIIADIGEGGKTIALRADMDALPVQEENDVPYKSKIPGKMHACGHDAHTAMLLGAAKIIAEHREELNGRVRLIFQPAEEGGNGAVKMIEGGALEGVNAIFGFHVWMDLPSGVIGIRDGPFLAGAGIFGGKIIGKGGHGASPHETVDPIPIMAETVLAFQAIVSRNVPPIETGVVSVTSVHGGTAFNVIPEEVEFKGTFRFFKPEIGELIQRRMREILEGVTKAHGAKYELSIEELTPPTINSKEMANFARKVAEKYGLKYGDVPPTMGAEDFAFYLQKVHGAFLALGIRNEEKGIIYPHHHPKFDVDEEVLYLGTAMEVALAFEFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P787</accession>
    <name>A0A218P787_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribonuclease J</fullName>
            <shortName evidence="8">RNase J</shortName>
            <ecNumber evidence="8">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rnj</name>
        <name evidence="10" type="ORF">A3L08_04420</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">An RNase that has 5'-3' exonuclease activity. May be involved in RNA degradation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 2 Zn(2+) ions per subunit. It is not clear if Zn(2+) or Mg(2+) is physiologically important.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. Archaeal RNase J subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853876.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P787"/>
    <dbReference type="GeneID" id="33315488"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04420"/>
    <dbReference type="OrthoDB" id="63419at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07714">
        <property type="entry name" value="RNaseJ_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10710">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01492">
        <property type="entry name" value="RNase_J_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004613">
        <property type="entry name" value="RNase_J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042173">
        <property type="entry name" value="RNase_J_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030879">
        <property type="entry name" value="RNase_J_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055132">
        <property type="entry name" value="RNase_J_b_CASP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001587">
        <property type="entry name" value="RNase_J_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00649">
        <property type="entry name" value="MG423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43694">
        <property type="entry name" value="RIBONUCLEASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43694:SF1">
        <property type="entry name" value="RIBONUCLEASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22505">
        <property type="entry name" value="RNase_J_b_CASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01292">
        <property type="entry name" value="UPF0036"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="9">
        <location>
            <begin position="14"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="382"/>
            <end position="386"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="444" mass="49621" checksum="1AE231B69DB3AEBE" modified="2017-09-27" version="1">MIKIYTLSGYEEVGKNMTAVGYTNGGREEVVIIDMGIRLDRVLIHEDVNIQEFPTKELQKLGAIPDDSVLRNKKVVAITFTHGHLDHIGAIGKLAPHYPDVPVYGTPYTIKLAKSEVKSEKYFEVKNPMYETQFGEIVQVSENLAIEFVQITHSIPQAAMVVVHTPEGAVVHTGDFKFDNNNPLGEKPDYKRLKELGKEGVKVLIPESTRVSEPTKTPSEAVAQMLLEDFFLYEGMEEDGLIATTFASHIPRLQELIWIANKMGRQAVFVGRSLAKYTGIAKQLGLIRMKGARAVRSPNAIKKVLAEVSGARENYLLVVTGHQGEPGAVLTRMANGELYDIGKRDTVVFSAGTIPNPLNKAQRYVLETKLKMKGVRMIKDLHVSGHASREDHRYLLRMLNPENIVPAHGEFRMLTHYAELAEEEGYLIGRDVFVSRNGYTVEIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7B0</accession>
    <name>A0A218P7B0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_04680</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853923.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7B0"/>
    <dbReference type="GeneID" id="33315540"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04680"/>
    <dbReference type="OrthoDB" id="102091at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="602" mass="69268" checksum="8968AFAEA0034599" modified="2017-09-27" version="1">MKEIGKVKRLDEFLTIKRNSSKSKDKSVTSSRTQQAETLNQESSFALIKGTIARTDRVSGLPHLDELTHHLISVATASLKFNSPETTLAALIHDYYKPVFDFRKENRWKWYHYITNQKTYVQLLSEFKDSINVFNVARISQWHHPNYKTRECNEICQIENSKLISALETSLPFALPQRDDYVIIQHLRVPGKYRVFLLALLKEMLSRELSKEYAKRFQKILGVSRIRYEYRPVQGLRRVEDVGKLIQENDWKIEIKENTMIIPLPSRFHKEVFYFEYYEGSEIVLDVDTKARMIRGVKVPFGMALSTIYFAGAQDAYLVYVDAGNGLLPLDALLGALIEDLKRSLSKNKQKTAFNSIDISKITMSLTGEFESDTMCVFCGEPGESISDNEKVKSIMANKFTDTWLLLTHGASVCPACKLGFEIEELFRAQGMNKYLAEEAILEMRLISTEIPIFRSENFLRSISSKIWLELLSEVYYSLHKSKELQKITKKNEWTTAFYLNPKVVIYPYIADMTPQVLSVALRYSKKKFVLQSGIHSRVVFPGNEKDMTSEEFAIVRKFYSSHPSIGLNLVHRIKSVYNPIFGVPKVAIQRGGGSRARKRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7C0</accession>
    <name>A0A218P7C0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_04740</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853934.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7C0"/>
    <dbReference type="GeneID" id="33315552"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04740"/>
    <dbReference type="OrthoDB" id="9623at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051081">
        <property type="entry name" value="HTH_MetalResp_TranReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054749">
        <property type="entry name" value="PF0095-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047699">
        <property type="entry name" value="sulf_reg_SurR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33154">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ARSR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33154:SF33">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR SDPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22315">
        <property type="entry name" value="PF0095-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="4">
        <location>
            <begin position="7"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="238" mass="27378" checksum="730E274F914DA8C0" modified="2017-09-27" version="1">MPETEPDVFYILGNKVRRDLLSHLTCTECYFSFLSSKVSVSSTAVAKHLKIMEREGILKSYEREGPFLGPARKYYEINISKTFVTTITPHIFWYRGLDLNEASTEKATIDISTVDTESGDLYTQIESFLQLTRELDKILSLLQAVEHKRDKLMARIKETYLNEIGDMTQLAILHYVLLNGEATIDELSDRLNLKEREVLVKAQELERFVPLKIKDNTIKIDEERLKQRIGGENDAGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7J4</accession>
    <name>A0A218P7J4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribonuclease Z</fullName>
            <shortName evidence="8">RNase Z</shortName>
            <ecNumber evidence="8">3.1.26.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">tRNA 3 endonuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNase Z</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rnz</name>
        <name evidence="10" type="ORF">A3L08_05210</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.</text>
            <dbReference type="EC" id="3.1.26.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the RNase Z family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.11" evidence="8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854007.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7J4"/>
    <dbReference type="GeneID" id="33315646"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05210"/>
    <dbReference type="OrthoDB" id="85118at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042781">
        <property type="term" value="F:3'-tRNA processing endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07717">
        <property type="entry name" value="RNaseZ_ZiPD-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01818">
        <property type="entry name" value="RNase_Z_BN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013471">
        <property type="entry name" value="RNase_Z/BN"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000801">
        <property type="entry name" value="PRK00055.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02651">
        <property type="entry name" value="RNase_Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46018">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46018:SF2">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23023">
        <property type="entry name" value="Anti-Pycsar_Apyc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="7 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="9">
        <location>
            <begin position="19"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="314" mass="36328" checksum="6CEF988892201D61" modified="2017-09-27" version="1">MLEVFFLGTGGIMPTKERNVPAIALRYKGEIILFDAGEGTMRQMNTAKLSPMKVEKIFITHFHGDHYLGLAALIQTMNLWDREKPLHIYGPKYTFEFVQHFLNSGFFRPGFDIHVHELGETRLKFGDYEIWSFKVEHGIPALGYVFKERDKRGKFLPEKLREYGLSEGPILGKLEREGQIEWNGRVIRLEDVTGPRKKGLKVVYTGDTEPAERVRLFAERADLLIHEATYLNPGDRGESYHSTVEEACDIARKAKVKLLALFHRAFRYTYEEYVREAERICGDFGVNFIVPKDFDVITFKSGSWELRNLLEERG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7K5</accession>
    <name>A0A218P7K5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dinitrogenase iron-molybdenum cofactor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854017.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7K5"/>
    <dbReference type="GeneID" id="33315656"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05260"/>
    <dbReference type="OrthoDB" id="85838at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00851">
        <property type="entry name" value="MTH1175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.130">
        <property type="entry name" value="Dinitrogenase iron-molybdenum cofactor biosynthesis domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003731">
        <property type="entry name" value="Di-Nase_FeMo-co_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036105">
        <property type="entry name" value="DiNase_FeMo-co_biosyn_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033913">
        <property type="entry name" value="MTH1175_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051840">
        <property type="entry name" value="NifX/NifY_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33937:SF2">
        <property type="entry name" value="DINITROGENASE IRON-MOLYBDENUM COFACTOR BIOSYNTHESIS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33937">
        <property type="entry name" value="IRON-MOLYBDENUM PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02579">
        <property type="entry name" value="Nitro_FeMo-Co"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53146">
        <property type="entry name" value="Nitrogenase accessory factor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dinitrogenase iron-molybdenum cofactor biosynthesis" evidence="1">
        <location>
            <begin position="14"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="130" mass="14597" checksum="BA753E52B33C017F" modified="2017-09-27" version="1">MRIAIPAEDDRGLESNVSGHFGRAKYFVFVDVEDNEIKGYEVVEVPFEEHGPGDLPSFVKEHGGEVVLAYGMGRKAMSYFNELGIAVVTGAYGPIKDVVKAFIEQVLEVDPNWKEKIEWEKEHRGECGEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7K8</accession>
    <name>A0A218P7K8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Small ribosomal subunit protein eS27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">rps27e</name>
        <name evidence="10" type="ORF">A3L08_05135</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8 9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 8">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the eukaryotic ribosomal protein eS27 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012571194.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7K8"/>
    <dbReference type="SMR" id="A0A218P7K8"/>
    <dbReference type="GeneID" id="74505856"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05135"/>
    <dbReference type="OrthoDB" id="5718at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.100:FF:000002">
        <property type="entry name" value="30S ribosomal protein S27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.100">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00371">
        <property type="entry name" value="Ribosomal_eS27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000592">
        <property type="entry name" value="Ribosomal_eS27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023407">
        <property type="entry name" value="Ribosomal_eS27_Zn-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001629">
        <property type="entry name" value="PRK00415.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01667">
        <property type="entry name" value="Ribosomal_S27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01168">
        <property type="entry name" value="RIBOSOMAL_S27E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 8 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5 8 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="zinc finger region" description="C4-type" evidence="8">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="65" mass="7040" checksum="5ECD3B5E716270BF" modified="2017-09-27" version="1">MALPKNLIPMPRSRFLRVKCIDCGNEQIVFSNPATTVRCLVCGATLVEPTGGKGVIKAKILEVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7L5</accession>
    <name>A0A218P7L5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HupF/HypC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854032.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7L5"/>
    <dbReference type="GeneID" id="33315672"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05340"/>
    <dbReference type="OrthoDB" id="43695at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902670">
        <property type="term" value="F:carbon dioxide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000022">
        <property type="entry name" value="Hydrogenase assembly chaperone HybG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019812">
        <property type="entry name" value="Hydgase_assmbl_chp_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001109">
        <property type="entry name" value="Hydrogenase_HupF/HypC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00074">
        <property type="entry name" value="hypC_hupF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35177">
        <property type="entry name" value="HYDROGENASE MATURATION FACTOR HYBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35177:SF2">
        <property type="entry name" value="HYDROGENASE MATURATION FACTOR HYBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01455">
        <property type="entry name" value="HupF_HypC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00445">
        <property type="entry name" value="HUPFHYPC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159127">
        <property type="entry name" value="HupF/HypC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01097">
        <property type="entry name" value="HUPF_HYPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="74" mass="8205" checksum="B4E32888D8899A57" modified="2017-09-27" version="1">MCLAVPGRIIEIEGKTAVVDFGGVKREVRLDLLPEAKVGDYVIVHTGFAIERLDEKRALEILEAWAEVERALEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7L8</accession>
    <name>A0A218P7L8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Iron-sulfur cluster carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_05305</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Binds and transfers iron-sulfur (Fe-S) clusters to target apoproteins. Can hydrolyze ATP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854025.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7L8"/>
    <dbReference type="GeneID" id="33315665"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05305"/>
    <dbReference type="OrthoDB" id="85513at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003784">
        <property type="entry name" value="Iron-sulfur cluster carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044304">
        <property type="entry name" value="NUBPL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961">
        <property type="entry name" value="IRON-SULFUR PROTEIN NUBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961:SF2">
        <property type="entry name" value="IRON-SULFUR PROTEIN NUBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="28"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="244" mass="27212" checksum="4EB634B09DB2480E" modified="2017-09-27" version="1">MIAGIDPREIAISARLENVKRIIPVVSGKGGVGKSLVSTTLALALAEKGHKVGLLDLDFHGASDHVILGFEPKEFPEEDRGVIPHTVHGIKFMTIAYYTEDRPTPLRGKEISDALIELLTITRWDELDYLVIDMPPGLGDQLLDVLRFLKRGEFLVVATPSKLALNVVRKLVQLLLEEKHRVIGIVENMKLDEEKDVEALAKEFGIPYLAGIPFYPDLDAKIGKVDELMKTEFAERIREIAEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7M8</accession>
    <name>A0A218P7M8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ATP-NAD kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05375</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854039.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7M8"/>
    <dbReference type="GeneID" id="33315679"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05375"/>
    <dbReference type="OrthoDB" id="56451at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039065">
        <property type="entry name" value="AcoX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011386">
        <property type="entry name" value="Put_ATP-NAD_kin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40697">
        <property type="entry name" value="ACETOIN CATABOLISM PROTEIN X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40697:SF2">
        <property type="entry name" value="ATP-NAD KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016907">
        <property type="entry name" value="Kin_ATP-NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="378" mass="41571" checksum="74C56509C79A38EB" modified="2017-09-27" version="1">MRVGLIVNPIAGMGGKVALKGTDGVVGEAIKRGARPIAADLVRLFLSELTHYEEARKIEFLTGPGPLGEDVLREFDFPYEVIRHREIGYHLVEGIRIPDTTSEDTKELSKSMLGKVGLLIFAGGDGTARDVVEAVDEKAPILGIPTGVKMYSGVFAASPEDAARVLVEFIRGKARLEEREVRDIDEEAYRHDEVKARTYGKALVPVVETLVQGSKEGVPLDEEDELEAIAEALAEEILENDGIYFLGSGSTIKRIKDALGIDGTLLGVDVVEVQGGRARLLVKDATEKDLLRFVDRNPKIVVTVIGGLNFLFGRGNQQFSAEVLRHIPKENIIVVATPSKVRNGFIRVYTGDREVDEKLRGYIKVRVSPWMERMVKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7S8</accession>
    <name>A0A218P7S8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Anhydromevalonate phosphate decarboxylase</fullName>
            <ecNumber evidence="11">4.1.1.126</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">A3L08_05655</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the conversion of trans-anhydromevalonate 5-phosphate (tAHMP) into isopentenyl phosphate. Involved in the archaeal mevalonate (MVA) pathway, which provides fundamental precursors for isoprenoid biosynthesis, such as isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(2E)-3-methyl-5-phosphooxypent-2-enoate + H(+) = isopentenyl phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:78971"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:65078"/>
            <dbReference type="ChEBI" id="CHEBI:229665"/>
            <dbReference type="EC" id="4.1.1.126"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:78972"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>prenylated FMN</name>
            <dbReference type="ChEBI" id="CHEBI:87746"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UbiD family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.126" evidence="11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854091.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7S8"/>
    <dbReference type="GeneID" id="33315735"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05655"/>
    <dbReference type="OrthoDB" id="8480at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1670.10:FF:000003">
        <property type="entry name" value="Phenolic acid decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1670.10">
        <property type="entry name" value="UbiD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002830">
        <property type="entry name" value="UbiD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049381">
        <property type="entry name" value="UbiD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049383">
        <property type="entry name" value="UbiD-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048304">
        <property type="entry name" value="UbiD_Rift_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00148">
        <property type="entry name" value="UbiD family decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30108">
        <property type="entry name" value="3-OCTAPRENYL-4-HYDROXYBENZOATE CARBOXY-LYASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30108:SF21">
        <property type="entry name" value="4-HYDROXYBENZOATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01977">
        <property type="entry name" value="UbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20696">
        <property type="entry name" value="UbiD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20695">
        <property type="entry name" value="UbiD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143968">
        <property type="entry name" value="UbiD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0288">FMN</keyword>
    <keyword evidence="8" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase-like N-terminal" evidence="15">
        <location>
            <begin position="5"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase-like Rift-related" evidence="14">
        <location>
            <begin position="87"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase-like C-terminal" evidence="16">
        <location>
            <begin position="285"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF20695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF20696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ASJ06841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="421" mass="47672" checksum="AFECFE0EB3AF4491" modified="2017-09-27" version="1">MLREIIERFEDAIIIEEPVSKELEVTRYLLKYRDRPVLFRDVGGWEVAGNIWSTRERIARYLGIKREEIIHFMADAMENPSPYRVVEKAPFLGNSTADFSLRELPVPKYYPKDGGQYFTSAMVIAKDENGFVNVSFHRIMVRDDKTAAIRLVPRHLYAMWKDKADHGEELDVRIVVGNPVHLLLAGATSTSYGISELEIASRMSEKAFGRPLEVVSVRGIPVPVETEFVFEAKITPELVDEGPFVDITGTYDYVRKQPLVVFERMYHVDEPIFHALLPGGYEHYMLMGLPKEPQIYASVKRVVPKVHGVRLTEGGAMWLHAVVSITKQHDGDGKNAILAAFAGHPSLKHVVVVDEDINIYDDREVEWAVATRFQADKDLIIVPNARGSSLDPSAEKSLTAKWGIDATKPLDRKEEFERAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7T0</accession>
    <name>A0A218P7T0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_05610</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S49 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854082.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7T0"/>
    <dbReference type="GeneID" id="33315726"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05610"/>
    <dbReference type="OrthoDB" id="31107at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07023">
        <property type="entry name" value="S49_Sppa_N_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004635">
        <property type="entry name" value="Pept_S49_SppA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002142">
        <property type="entry name" value="Peptidase_S49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047272">
        <property type="entry name" value="S49_SppA_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00706">
        <property type="entry name" value="SppA_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42987">
        <property type="entry name" value="PEPTIDASE S49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42987:SF4">
        <property type="entry name" value="PROTEASE SOHB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01343">
        <property type="entry name" value="Peptidase_S49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S49" evidence="7">
        <location>
            <begin position="147"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="332" mass="36565" checksum="A4A7629CAF4709F5" modified="2017-09-27" version="1">MREDIWKYISAVLILLLAVSSVAVVLLYMQNSELKSYPSNASPVVVETSLNATCNETAYKLQIDELQRQLEFLKAQLREENMPEGNTTIAIVPIFGLIDDYTALQVIPVLRGIAKNDSIGGVVLWIESPGGYVAPVREIYSTVRKLDLIKPVVAYTGGMAASGGYYIAVGAEKIIADPLAEVGSIGVIYVHYDLQKNYEMNGVKVDVFKTGPYKDMGAEWRGLTDEERKMIAESIDTHFQAFLQAVSTGRGMPLNETRKYATGQTWFAMNVTGSLVDETGDLDYAIKVLSETLNISKPRVVIYKGERSSDFGIFGSTALLLDPRYVSAYIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7U1</accession>
    <name>A0A218P7U1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Radical SAM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854108.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7U1"/>
    <dbReference type="GeneID" id="33315753"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05745"/>
    <dbReference type="OrthoDB" id="358785at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.320">
        <property type="entry name" value="Photosystem II 12 kDa extrinsic protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43324">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43324:SF1">
        <property type="entry name" value="RADICAL SAM CORE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81585">
        <property type="entry name" value="PsbU/PolX domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="1">
        <location>
            <begin position="196"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="581" mass="64980" checksum="6230376B5175FB69" modified="2017-09-27" version="1">MIVAIIDGYTDEPAGLGVPPYLGIYPRYAYGAIKKARKDARVFYLTIDDLRAAFEGENGIATKNKTPNFPKAREILEKADVIIYIGGLHTPGKYLSAVPSQVEEVARFIKPFGGVKILGGPAFMGSAHAGGTKITSRELLLAQSIFDHIVYGDLEAFLFDYLTNPKDADPFRFRNYNELRDYAIIGAEVVKQFPDFPDFVIVEIETQRGCPKAMGIGGCSFCTEPVRYRTVEDRPIEDIVAEVKALYDLGVRHFRVGRQSCIFSYMAKPNGRVPVPNPDALEKLFRGIRSVALDVKTLHVDNANPAVIANYPEESIRIAKALIKYGTPGNVVAFGLESADPKIARLNNLNATAEETYEAVRILNEVGARRGPNGMPWLLPGINIIFGLPGETKKSYELTFQLLKRLLDDGLMVRRINIRQVVVFPGTPLWHLRSKIKTEKHKKLIQHYKYKIRHEIDLPMLRRVVPVGTILRDVRAEVFDNGLTYGRQIGSYPLIVGIPKEMPLNRFYDVLIVDHGFRSITGIPVPINVNTESPRVLGYLPGIGKKRAVKILASRPFRTKEEFFSVADGKNREMLEGLITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7W6</accession>
    <name>A0A218P7W6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854080.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7W6"/>
    <dbReference type="GeneID" id="33315724"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05600"/>
    <dbReference type="OrthoDB" id="84938at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.20">
        <property type="entry name" value="Ribonuclease III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038133">
        <property type="entry name" value="Ribo_III_sf_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021568">
        <property type="entry name" value="Ribonuclease_III_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11469">
        <property type="entry name" value="Ribonucleas_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="128" mass="14421" checksum="987F357980A1718C" modified="2017-09-27" version="1">MRYEKNFTDKGLAKFGDSLVNFTFSLALSEYLEKPTGERVPNASLAMALELAGLRNAIPPRTDKHGKGDIAEAIFAYAWLEGKITVDEAARILRANFTEDVTHFSRRKEVIGRAFAEVFKVIKERLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7X3</accession>
    <name>A0A218P7X3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_05650</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854090.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7X3"/>
    <dbReference type="GeneID" id="33315734"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05650"/>
    <dbReference type="OrthoDB" id="87732at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025302">
        <property type="entry name" value="DrrA1/2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43335">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43335:SF4">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13732">
        <property type="entry name" value="DrrA1-3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="2"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="305" mass="34632" checksum="31404ECFE88D49A3" modified="2017-09-27" version="1">MIRIENLVKLYKDVRALDGLSLEVKPGQVYGFLGPNGAGKSTTILSTLGLIFPQEGRIELFNLEVFRDGKFNEDDLVEAKRRIGYMPEHATLWDFMTPAQTLDVIGEAFEIPKAEREKRIRELLELVNLWEDRNRKVGKFSKGMRQRLLLAQALINDPDLLILDEPMTGLDPKGIAEFKEIIAAQKKAGKTVFFSSHILAHVEEVCDTVGVIVKGKLRVEDNLERIKREFLKKAGYTIILETNVPVDFTGVEWKVSPLGEKKYRIVAPEDIREELHDFVSAKGAKILAMQVKEPSLEEIFLKMVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P804</accession>
    <name>A0A218P804_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">S-layer protein outer domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05755</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854110.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P804"/>
    <dbReference type="GeneID" id="88621811"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05755"/>
    <dbReference type="OrthoDB" id="92388at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022651">
        <property type="entry name" value="S_layer_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006454">
        <property type="entry name" value="S_layer_MJ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01564">
        <property type="entry name" value="S_layer_MJ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05124">
        <property type="entry name" value="S_layer_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S-layer protein outer" evidence="1">
        <location>
            <begin position="29"/>
            <end position="1048"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="1051" mass="114510" checksum="AD5F54B2A48C6082" modified="2017-09-27" version="1">MKVKKIAALAVGAAMVGATIGFASAQPTVPEIPKDFFVKDGQPNVKIVVGSQGAALDVASAADIAVAIGSMLYTEKEVDANGTSVVLKKDISGDWDIADLPVFGGDVLGKYAVISEDAYQDPEEYKSEWEYNWYGVPLYTGDVPVAVKLMTSAGEVQVPLDGSAVTGPDGREYKVLKYYNLGEDTGTVVLDIDGKTYALKLGETQILEVVPEYEGEITPSGDITLPIMASDPETGDIVNLILETNPNLQFAPKDDDQSPLTLAEGEKLIADLTTEGYTGHYFKFNASNIDPVGDTGDLTISWDNTSVTVNEVYLNGQQVTVTGNSTTFTGLTIGDIIEFYTDEGIFDFQVVEMNQTGNPAVDYMRLNFDLSAIGGYQNEYTTNSFPASTYLDLKEGEVQDNVNITTVIPGNVTFEIKDVDTNEDNVKIAIESVYYNGVKTLVDWYREFVMLTDEQQHMGVPTSVGSVTFDMYVNYVGSRPNTISVRLGDWDTLAEGEWFLVGDFNVTVEDIDLGDGEVTLHISTIPGSDIIVDQDVVVDLNDEVLADLEFSMESIEISVDLVSEGVTFESVVWEENTERGYGKFGDSYWADGTWDEETIGPGIVSNFIEWNTHQGDYPDTYSEEATFEISQIEIKDLKKYVDKNIVPPEDATVVIPEGGISITVDLGYDVVTYQYCSNPYCEYLGGDPDVAGEYPTASSDGHVNGAYEGDTLTIVPFEKTVSILDIGDDYFTYGVDYGSQWYKVGDEKTFDDYVLKVLDINIFEQKVLLQLSEVGGEPVIRSVKVGDTTKLGGLYVTVEDAFIGATQDVIAKLSVKVEEGKVVSGSEKYSLAPGYVTHLDIKEDSAGYKYIDKITFVNSEPIEGETIDIFNTYEIDYVYTAKEVNCVKGDDEFTKYLVNAEVVIDPYPYYIYETLEVGDEVGAADNEHTGWIVDAITAEKYTQVTPGEAKPITVLDTEIMEAGLESVDSNLILVGGPVVNSVAAALAEKLEIPTDYDGWESEYGTGADSGVIKYIAEVSDINGYGVVLVAGTDREGTQAAAEALMEYLAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P816</accession>
    <name>A0A218P816_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADH-quinone oxidoreductase subunit D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_06175</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the complex I 49 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854186.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P816"/>
    <dbReference type="GeneID" id="33315838"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06175"/>
    <dbReference type="OrthoDB" id="43567at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="entry name" value="Cytochrome-c3 Hydrogenase, chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014029">
        <property type="entry name" value="NADH_UbQ_OxRdtase_49kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022885">
        <property type="entry name" value="NDH1_su_D/H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004739">
        <property type="entry name" value="PRK06075.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993:SF10">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 49 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="HydB/Nqo4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00535">
        <property type="entry name" value="COMPLEX1_49K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADH-quinone oxidoreductase subunit D" evidence="6">
        <location>
            <begin position="139"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="391" mass="44584" checksum="2B9CBA0C90C2E1B3" modified="2017-09-27" version="1">MVSQEELIREARANGMDLLPLEKDTYELFFGPQHMATENYSLILKMDGHRVVKAIANPGFLHRGFEKLAEYRPWHTNIALLLRICVPEPDVPEAIYSMAVDEIMGWEVPERAQWIRTTVLEMARVSAYLFWIMGLSFKLGVYTAGQWAAAYRERFMALFEQLTGARVYHIYTIPGGVRRDIPGDKWLRQLRDTVEYLKGKLKDFDNILFENYITYRRLEGIGVMDKKFALEEGVTGPNLRATGVPYDVRRVDPYLLYPELDFEVPVLREGDALARALIRRFEIEQDLYILEQLLDMGPPSGPYKVDHPGFKALPRLKVPAGDAFAHVEATKGDFGAYVVSDGKNKPYRVQIRGPSIAHGIRVIEQLLVGARIADVPVILMSLDNCPPDIDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P828</accession>
    <name>A0A218P828_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glutaredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06125</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854178.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P828"/>
    <dbReference type="GeneID" id="33315828"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06125"/>
    <dbReference type="OrthoDB" id="73564at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="180" mass="19979" checksum="658A1D2A976E3E85" modified="2017-09-27" version="1">MKKLGMVLLLMLLIGFAAGCISNSNTSTQSQTTSTGPEYVVINGTKIYLNQIHFYMYGLKTCPHCRAMKEEIPKVYGNDSLTYYELMNNETNMKLFETQYTYTGISGVPAIAIAYGGNLTAIIEGEYNVSATPKIIYTSMENRAVVLFVGGKAYLLKNQTIISTLKAIYIEHRLPENETG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P860</accession>
    <name>A0A218P860_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854161.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P860"/>
    <dbReference type="GeneID" id="33315809"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06030"/>
    <dbReference type="OrthoDB" id="101521at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="139" mass="15541" checksum="6C130CECBA462604" modified="2017-09-27" version="1">MVIDVPIHEEIETEVPLDTYVTVPVKLVTRARVVQNITTTVDINGEQKEIIVPLDTYVDVPIDTEVRVHFKDTFPVTVPLDMTVEVPINKTVVVPFDETFKVPVHMEVKIEKTLQELGLGDLIDQIVEILLDVKSALGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P874</accession>
    <name>A0A218P874_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_06420</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854231.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P874"/>
    <dbReference type="GeneID" id="33315887"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06420"/>
    <dbReference type="OrthoDB" id="46231at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051011">
        <property type="entry name" value="Metal_resp_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033788">
        <property type="entry name" value="HTH_metalloreg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43132">
        <property type="entry name" value="ARSENICAL RESISTANCE OPERON REPRESSOR ARSR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43132:SF2">
        <property type="entry name" value="ARSENICAL RESISTANCE OPERON REPRESSOR ARSR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="4">
        <location>
            <begin position="34"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="125" mass="14655" checksum="C495C5B571CEFAEA" modified="2017-09-27" version="1">MKVRDILEKLDEKQKKTVMRCKETCGIPCIDKEIDAEVEEDIVKFLKAVSNPLRLKILKLLKDNWLCVCVIASALDQDQTLISHHLRTLRSLGLIEVRKEGKMRFYRTNKEVLERYLQRVTSELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P879</accession>
    <name>A0A218P879_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Metalloprotease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_06425</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854232.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P879"/>
    <dbReference type="GeneID" id="33315888"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06425"/>
    <dbReference type="OrthoDB" id="4612at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08072">
        <property type="entry name" value="MPN_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028090">
        <property type="entry name" value="JAB_dom_prok"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14464">
        <property type="entry name" value="Prok-JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 7" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="JAB" evidence="6">
        <location>
            <begin position="9"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="123" mass="14246" checksum="4E98DFCB2831E0B9" modified="2017-09-27" version="1">MDVVRIRRELLEYLLELARDFYPNEFAGFLREKDGVFEEILIAPNPHFGRSSAFFDTWMLPYDESIKGTVHSHPGPSPRPSRADLHFFSKFGGVHLIIAYPFTEEDVRAYLSNGEEVRIEVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P884</accession>
    <name>A0A218P884_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Formate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_06500</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854246.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P884"/>
    <dbReference type="GeneID" id="33315903"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06500"/>
    <dbReference type="OrthoDB" id="23466at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02775">
        <property type="entry name" value="MopB_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.90">
        <property type="entry name" value="ADC-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.228.10">
        <property type="entry name" value="Dimethylsulfoxide Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050123">
        <property type="entry name" value="Prok_molybdopt-oxidoreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105:SF10">
        <property type="entry name" value="NADH-QUINONE OXIDOREDUCTASE SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105">
        <property type="entry name" value="RESPIRATORY NITRATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53706">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase, domains 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molybdopterin oxidoreductase" evidence="4">
        <location>
            <begin position="59"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Molybdopterin dinucleotide-binding" evidence="5">
        <location>
            <begin position="539"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="623" mass="68667" checksum="E7C7D606921B7B86" modified="2017-09-27" version="1">MRTIVTCFHCGIGCRVEVKKKRSRPHEINYLRDLNVPNYYGKLCSRGNLMFEPTVENKRIKRPMKALEEGKFVEISWADALREVSFQLSRYARDDPSMLGFIGGETVSNESAYLFQKLARLLGTNNIDTTASLSQGVLIRPVLDMGAWEHWADFADIDGADLIIVWGADLARNYPVLLGRIARARERGARVVLVDPVNGRASKFADFYLRPLPGTDVFLALSIMNYLVKTDERFGAVLPEDVLKLVSRSPPSYGEELTGVQERDIKAVAHEILRSSGGIILLGSGAVMNENGASFVRAIITLAFVSGMKFLPISRYGNSQGVLDMGLLPDLLPGYTPYSEGRDFQKAWLVEGLNPNPGKSLAEMLDGGINVFYLLGADLIRHIPGALDALRNAEFIIMQDSFMTETAKFADIIFPSALLYEEGGSTTNSERRVLWCEKAKRPPGEARGAVSILGEVGKAINLPGFNYTFPEEVLREISLLIPDYPGPRELVGSPEGALLKRPNGVKRKFVPLIPAKHPEGEVLLIRGHSGRFMPELLAGKMGETDVVLISPEDASRLGVSNGDRISIEVDGNRITVRAKVSNRTLKGVAVVHWDLVRAALPAKSSENCLVSRIYPCRIRGDEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P886</accession>
    <name>A0A218P886_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coenzyme F420 hydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_06610</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854266.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P886"/>
    <dbReference type="GeneID" id="33315925"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06610"/>
    <dbReference type="OrthoDB" id="37898at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052592">
        <property type="term" value="F:oxidoreductase activity, acting on CH or CH2 groups, with an iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007516">
        <property type="entry name" value="Co_F420_Hydgase/DH_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045220">
        <property type="entry name" value="FRHB/FDHB/HCAR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007525">
        <property type="entry name" value="FrhB_FdhB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31332">
        <property type="entry name" value="7-HYDROXYMETHYL CHLOROPHYLL A REDUCTASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31332:SF0">
        <property type="entry name" value="7-HYDROXYMETHYL CHLOROPHYLL A REDUCTASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04432">
        <property type="entry name" value="FrhB_FdhB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04422">
        <property type="entry name" value="FrhB_FdhB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Coenzyme F420 hydrogenase/dehydrogenase beta subunit N-terminal" evidence="1">
        <location>
            <begin position="15"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Coenzyme F420 hydrogenase/dehydrogenase beta subunit C-terminal" evidence="2">
        <location>
            <begin position="102"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="276" mass="30750" checksum="D9B82EE8FBBF7190" modified="2017-09-27" version="1">MISVSNSFVGHVFSIYLARSTDEEVINTKVASGGAVTAILSYALDTGLIDGVVTAKRTSGLEGEAVVARSRKELLATVGNKWSIVPLASRIRAKIEENDLNQVSVVCLPCQAQFFGQMRDFPMLETDFGERIEYIISLFCMGTFAFETFTNYLYRNHGIRGRDVKDIILRGDVIEVHHPGGVLRLSIRDIYQHLQVGCLVCSDYTGNWSDISAGIVETKPGWTMLIARNKKADELIKNAKKHGYIEVTDGSPFIGDVITRAREKLTRSQKNMMYLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8A1</accession>
    <name>A0A218P8A1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06580</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854261.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8A1"/>
    <dbReference type="GeneID" id="33315919"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06580"/>
    <dbReference type="OrthoDB" id="87283at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="107" mass="12174" checksum="E4481D4266550E60" modified="2017-09-27" version="1">MIEEFRKHYGENLLGIALLGETWLVVLKEGDKVELLADAAETWEGLDVIAVPVSSIHNIHPEVFGDFQVLYDPEGIVSRSLERIMELRGAYPTLWNLKLIEVTEVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8F0</accession>
    <name>A0A218P8F0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cysteine--tRNA ligase</fullName>
            <ecNumber evidence="12">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cysteinyl-tRNA synthetase</fullName>
            <shortName evidence="12">CysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">cysS</name>
        <name evidence="15" type="ORF">A3L08_06865</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>tRNA(Cys) + L-cysteine + ATP = L-cysteinyl-tRNA(Cys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17773"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:9679"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78517"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="12"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854312.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8F0"/>
    <dbReference type="GeneID" id="33315976"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06865"/>
    <dbReference type="OrthoDB" id="9445at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00672">
        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000009">
        <property type="entry name" value="Cysteine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1910">
        <property type="entry name" value="Cysteine-tRNA ligase, C-terminal anti-codon recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00041">
        <property type="entry name" value="Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015803">
        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015273">
        <property type="entry name" value="Cys-tRNA-synt_Ia_DALR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890:SF3">
        <property type="entry name" value="CYSTEINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="CYSTEINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09190">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00983">
        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00840">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 12 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Cysteinyl-tRNA synthetase class Ia DALR" evidence="14">
        <location>
            <begin position="356"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="306"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="12">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="12">
        <location>
            <begin position="266"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ASJ07062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="476" mass="56013" checksum="F14E5AF0D37D3480" modified="2017-09-27" version="1">MAIKIYNTQTRQKEEFRPLREGEVRMYVCGPTVYDYTHIGHARTYIAFDVVRRYFEHKGYTVLMVMNFTDIDDKIIKRANETGEDPKKLAEKFLRYFLEDMAALKVKPADIYPRVTEHIEDIIDFIRKLQEKGYAYEGSDGVYFEVRKFKDYGKLSKIKLEDLVKGARVEPGEGKKNPEDFALWKKAKPGEPKWESPWGEGRPGWHIECSTMSTKYLGESFDIHGGGNDLIFPHHENEIAQTEACTGHEWVRYWMHTGFLMVNGEKMSKSLGNFITIREALKRYDPEVIRLFVLQRHYRSPLDYTEEGMEHAKNNLERLYNTLENIRVAMERAEISFRWEEPEFEAYEAVREARKKFYEAMDDDFNTAEALKAVFEASNAVNRYLTQVETPKESILRKAWEFFKVVSEVFGIFEDYFKEQKAGEEEELIKLLVEVRAQLRKERNFALADKIRAELRELGIQLEDTPQGTVWKRVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8F1</accession>
    <name>A0A218P8F1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Radical SAM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_06790</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854297.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8F1"/>
    <dbReference type="GeneID" id="33315961"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06790"/>
    <dbReference type="OrthoDB" id="105445at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005909">
        <property type="entry name" value="RaSEA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01210">
        <property type="entry name" value="archaeosine biosynthesis radical SAM protein RaSEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF0">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004954">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="12"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="324" mass="37018" checksum="D3BB49EEEDF21458" modified="2017-09-27" version="1">MTYWTSEDNVAGKPGTALFIILPTIGCYRFRIGEACYMCAYPTAAPKIKWSQEAIVDYVREALKKIEGKKGPFAVRMFTSGSFLDNGELKPETRRKIFEILAKMDNVEEIVIESRSELVRYDAVKELAEIVPDKHFEVAIGLETANDDIANISINKGNTFADFVKAAEIVHKAGAKVKTYLLLKPIFLSERDGIRDAKESIIKAEPYTDTFSINITDIQKGTLYERLWEKGEYRPPWLWSAVELLIWAKRKFPEKRILSDPVGAGSKRGPHNCLTDYDRVIGRAIKKFSATQDLSHIESLKPECRERWEYIVENGLLDWQLVTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8G2</accession>
    <name>A0A218P8G2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glucodextranase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_06970</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_232461786.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8G2"/>
    <dbReference type="GeneID" id="69102010"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06970"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027552">
        <property type="entry name" value="CGP_CTERM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013319">
        <property type="entry name" value="GH11/12"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04288">
        <property type="entry name" value="CGP_CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="384" mass="42609" checksum="E22369CCC56D2F95" modified="2017-09-27" version="1">MRRRMIKAVSFFVLSLVFLGFVSAMTLEDIKGIAAAPAQEGDVQFTWWDQWLENPIQLSDGRTIWMQTNFWNIVDGQGEVYMRFIKSTEEVAFYVDLSDVQTQWGGIAWATPEVIIDGRAPAQWWGDKPPVGGYSHFQLPLPASDVSTHDSIWVKVKYDVARRDDTLVRYGINIWFEDKGSGAPIGEIYIPFFDDFGGIVGDRTDVGEVTSTVILNGEMKNIKFTIQRALSGGGWGVLLFMPQEQLPASGEVIIDIKPMIDKVIEQLDGFFGIPVDSQQWTSISIGSYSGSEGTAFNYGWILHDARILSPSEAPKIIKTTETVTKTETMTITETKTETKTETVTETKTETKTETKTETTGGGGICGPAAIIGLAIVPLLLRKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8G7</accession>
    <name>A0A218P8G7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-agarase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06975</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854332.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8G7"/>
    <dbReference type="GeneID" id="33315998"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06975"/>
    <dbReference type="OrthoDB" id="350155at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="498" mass="56906" checksum="284202A0066DC494" modified="2017-09-27" version="1">MRNTSFLKVLGFILVSTMVLGCIGNNAPTNSEGIDSSSYTQPATTDSSESMNSPSPTGSTVTNSPKPKANFSGEYSDFGGWKKVQVESLGFFRVEVVNGTYWLVDPEGYLFVSKGVNAINYLGDHSPKIGYAPYYVNVLRKYGDISVWINVTVNRMVEWGFNTVGSWSSPELYEYFPYTVLLDIGAKYGFNWEHGTMPDIFKDDFEEYVAKAVYFNVEPLKDNPMVIGFFTDNELRWGPDWRSGNHLLDDFMKLPPEAPGKRVAVEVLKETFNGDITRVNEELKTSYESFDDLLTYTGDLPSTPLFMEARKEFVRRYAERYFNVTTTAIRKVDQNHLILGVRFAVSPFIRPPDVVFEVAGKYVDVISLNLYNFQVAPKEYLDHIHEITGKPIMITEFSFRAMDSGLPNTIGAGITVQTQKERAEYTKRFIESFMELPYAVGYHWFKWSDQPKEGRWDGENSNYGLVNIEDEPYEEMVRMFSELNRNIEEIHLKGYEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8H8</accession>
    <name>A0A218P8H8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0127 protein A3L08_06750</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_06750</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0127 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854290.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8H8"/>
    <dbReference type="GeneID" id="33315953"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06750"/>
    <dbReference type="OrthoDB" id="64208at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1140">
        <property type="entry name" value="Protein of unknown function DUF192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00263">
        <property type="entry name" value="UPF0127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003795">
        <property type="entry name" value="DUF192"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038695">
        <property type="entry name" value="Saro_0823-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022906">
        <property type="entry name" value="UPF0127"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002996">
        <property type="entry name" value="PRK03760.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02643">
        <property type="entry name" value="DUF192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="162" mass="18343" checksum="CCFAC6A360EAF1C5" modified="2017-09-27" version="1">MITNETKGRVWHGPVRMADTFFKRFRGLMLAKNINYALVFVLPAETKANASIHMFFMLSEIDVIWLDSARRVVDFKRAKKWRLYSPKKAAKYIIEGPVGLIRTLEVEEGDLIDWSPTEEKGKAIPVKSLLPGKIDLNGSKNGVAMVESVKEVRADSPRPREG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8K6</accession>
    <name>A0A218P8K6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Multidrug-efflux transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_07190</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854372.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8K6"/>
    <dbReference type="GeneID" id="33316041"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07190"/>
    <dbReference type="OrthoDB" id="214119at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13137">
        <property type="entry name" value="MATE_NorM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050222">
        <property type="entry name" value="MATE_MdtK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048279">
        <property type="entry name" value="MdtK-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43298:SF2">
        <property type="entry name" value="FMN_FAD EXPORTER YEEO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43298">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN NORM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="163"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="279"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="398"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="425"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ07121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="456" mass="50421" checksum="44C5663B99C294D2" modified="2017-09-27" version="1">MIHLNEHQRRLWALAWPAIMGNISQTLLNLVDMMMVGQLGALALAAVGLGGQVSWFMMPIMMAVSTGTLALVARFVGAKDQENATLALEQSLYLAFLLGIPVMLFGWFFGDDILRIMGAGPDVVELGYDYLRVVFAFYPVRFVGFTAYSALRGAGDTKTPMKLGILMNVINAVFDYLLIYGKLGFPRLGAVGAAWASGIGITTSMLIVLYLLWRGKLVLMFKPSWSFHPEMAKRILRIGFPTMVERGLFSFYNFLYMSIVTRFGTIALAAHQVGLRVESIAYMPAFGFNVAASALVGQSLGEGKPEKAERFVYEALKMVGLFMGIMAFILIVFPRYLVMPFISPSDPHYGEVMRLASIYLIIVGISEIPLGWLFVLSGALRGAGDTKTPMYITAVSKLLFRIIPAYILGFGFTIGQIHFEGLGVVAAWIAMSLETFATAALFWWAFKRGKWKYVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8L1</accession>
    <name>A0A218P8L1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_07100</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854354.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8L1"/>
    <dbReference type="GeneID" id="33316023"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07100"/>
    <dbReference type="OrthoDB" id="46231at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051011">
        <property type="entry name" value="Metal_resp_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43132">
        <property type="entry name" value="ARSENICAL RESISTANCE OPERON REPRESSOR ARSR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43132:SF2">
        <property type="entry name" value="ARSENICAL RESISTANCE OPERON REPRESSOR ARSR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="5">
        <location>
            <begin position="45"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="136" mass="15643" checksum="0A703C2908D06EC2" modified="2017-09-27" version="1">MEDLKVQLEELKKRLEVLEENIDPVDEVMLSIKARLRRKLEGGKLPELDEEKAAKTLKALANPDRIRILKMLSKSPMGFKNIKEALGVESPTVSHHLKLLMKTRMVKKGEKYEISPNGRLFLRLLEIITALEEVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8L2</accession>
    <name>A0A218P8L2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">tRNA (guanine(26)-N(2))-dimethyltransferase</fullName>
            <ecNumber evidence="7 8">2.1.1.216</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">tRNA 2,2-dimethylguanosine-26 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNA(guanine-26,N(2)-N(2)) methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNA(m(2,2)G26)dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">trm1</name>
        <name evidence="10" type="ORF">A3L08_07200</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>guanosine(26) in tRNA + 2 S-adenosyl-L-methionine = N(2)-dimethylguanosine(26) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43140"/>
            <dbReference type="Rhea" id="RHEA-COMP:10359"/>
            <dbReference type="Rhea" id="RHEA-COMP:10360"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
            <dbReference type="EC" id="2.1.1.216"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.216" evidence="7 8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854374.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8L2"/>
    <dbReference type="GeneID" id="33316043"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07200"/>
    <dbReference type="OrthoDB" id="372177at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160104">
        <property type="term" value="F:tRNA (guanine(26)-N2)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002940">
        <property type="term" value="P:tRNA N2-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000272">
        <property type="entry name" value="tRNA (guanine(26)-N(2))-dimethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.70">
        <property type="entry name" value="N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00290">
        <property type="entry name" value="tRNA_dimethyltr_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002905">
        <property type="entry name" value="Trm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022923">
        <property type="entry name" value="TRM1_arc_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042296">
        <property type="entry name" value="tRNA_met_Trm1_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00308">
        <property type="entry name" value="TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631">
        <property type="entry name" value="N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631:SF3">
        <property type="entry name" value="TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02005">
        <property type="entry name" value="TRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51626">
        <property type="entry name" value="SAM_MT_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 8 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8 9" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="1 8 9" id="KW-0820">tRNA-binding</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ07123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="378" mass="42642" checksum="1D39BD15FFE91320" modified="2017-09-27" version="1">MEFVEVREGLARILVPKAERIYDAPVFYNPVMALNRDVSVLAVKIIQPGKALDALSATGIRGIRYALETPTGEVWLNDISEEAFKLILRNVELNLGVEGKPVGEKRVAFEGEKRIVANLDDANRLMAENFRYFDFLDLDPFGSPVEFLDTALRSVKRRGVLAVTATDTGVLCGAYRNACLRKYLAEPIRGDLCHEAGLRILIGTVVRYAAKYDLGVDVLLAYYRDHYFRAFLRLKSGAKKADKSLSQLGYLWQDENGRFEYAEGFLPERPKAHGPLWLGPLKDGEFVERLVEAAREHPLAHRKTLPFVELLNEELDVPFLYDTHALARRNGLQARKLSDMIEILEGKGYKATRTHFSPKAIKTDAPIEKVLEALRVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8P6</accession>
    <name>A0A218P8P6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_07475</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8P6"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07475"/>
    <dbReference type="OrthoDB" id="97955at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="79" mass="9299" checksum="7D758FC32B945406" modified="2017-09-27" version="1">MYAMDFDYYVLELDSVDGPLRVEVQFEAGSFRFIIADGYDRYIYEAVDLTPENVFLLAYVLSKYIERVLGEEEIRALLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Q7</accession>
    <name>A0A218P8Q7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HAD family hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_07495</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854426.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Q7"/>
    <dbReference type="GeneID" id="33316102"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07495"/>
    <dbReference type="OrthoDB" id="63793at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01427">
        <property type="entry name" value="HAD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="148" mass="15960" checksum="0FF59B7E3EA7A329" modified="2017-09-27" version="1">MEVPNYGKVDFKAVLFDLNGTLGERGRINEEVKHLLERLADKYTVVVLSADTFGTLEEELKGLPVRVERVSNGVEKAEIAKGYAPYAALGNGNNDVAMLEGAELAFCVIGPEGATVDALLASDIVVKDVRDAIAMLLDERKLIATLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8T2</accession>
    <name>A0A218P8T2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">3-methyl-2-oxobutanoate hydroxymethyltransferase</fullName>
            <ecNumber evidence="5">2.1.2.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Ketopantoate hydroxymethyltransferase</fullName>
            <shortName evidence="5">KPHMT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">panB</name>
        <name evidence="9" type="ORF">A3L08_07520</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = 2-dehydropantoate + (6S)-5,6,7,8-tetrahydrofolate</text>
            <dbReference type="Rhea" id="RHEA:11824"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5 8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodecamer; pentamer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the PanB family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.11" evidence="5"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854429.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8T2"/>
    <dbReference type="GeneID" id="33316107"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07520"/>
    <dbReference type="OrthoDB" id="8414at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003864">
        <property type="term" value="F:3-methyl-2-oxobutanoate hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06557">
        <property type="entry name" value="KPHMT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000003">
        <property type="entry name" value="3-methyl-2-oxobutanoate hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00156">
        <property type="entry name" value="PanB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003700">
        <property type="entry name" value="Pantoate_hydroxy_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00222">
        <property type="entry name" value="panB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001452">
        <property type="entry name" value="PRK00311.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20881">
        <property type="entry name" value="3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20881:SF0">
        <property type="entry name" value="3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02548">
        <property type="entry name" value="Pantoate_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000388">
        <property type="entry name" value="Pantoate_hydroxy_MeTrfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 9" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="5 6">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <begin position="44"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>3-methyl-2-oxobutanoate</name>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>3-methyl-2-oxobutanoate</name>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>3-methyl-2-oxobutanoate</name>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000388-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000388-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000388-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="284" mass="31787" checksum="366680DB5BF1D46A" modified="2017-09-27" version="1">MREITPRHIIEMKGKEKIAMVTAYDYPSALIADKAGMDIIFIGDSLGMVVYGEPNTLNVSMEQMVFHTRAVAKAVKRALVLADMPFGSYEVSVEEGVKNAVRLIQAGADAVKIEGGYDHRKLVKKLVRMGIPVMGHTGLTPQRYLRLGGYRLMGEIEEEIEEILRDAKALEKAGAFAVVLEFTLADVAKLVTEEISIPTIGIGSGPWVDGQVLVWHDLLGIYEDVPPFVKKYADIGGMMRLALENYREEVKAGKFPAKEHYWEFLDKDDFRRKAQKALEKLEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8T3</accession>
    <name>A0A218P8T3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-binding protein MutS2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mutS2</name>
        <name evidence="7" type="ORF">A3L08_07580</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Has ATPase and non-specific DNA-binding activities.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DNA mismatch repair MutS family. Archaeal Muts2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854440.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8T3"/>
    <dbReference type="GeneID" id="33316119"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07580"/>
    <dbReference type="OrthoDB" id="15514at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002865">
        <property type="entry name" value="DNA-binding protein MutS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00971">
        <property type="entry name" value="MutS2_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012401">
        <property type="entry name" value="DNA-bd_MutS2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF125">
        <property type="entry name" value="DNA-BINDING PROTEIN MUTS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029254">
        <property type="entry name" value="MutS_C_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="6">
        <location>
            <begin position="386"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="178"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="393"/>
            <end position="400"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="576" mass="65884" checksum="9361F8D131A0BBD8" modified="2017-09-27" version="1">MRLKLNPEAKTVYRSIQEEIKRRLVLPESASLLDEFGPVSDREEILRRQAYFRGTLPWIRPELKDYLSKVRHIKFRRDFLHDRILVVDESELERAEKLGLCEVTTEIEDTEGYPLVLSTAGYGIDVELVPSQIAPELYVMPLWENLETLKALAKIGELTGRESVAGEILRSLEGLREVMEKRKTLDDLEELIVEKERELNERISEKLERFSLTLSGKELLEFLSELKVGNYEAIFSHFGEVEGEILDLINEAENELSERIGITIELFSREELYPVSVPPERVEMLREELEKELKVELYLRSREILEEVMPLLQRLKEELSAVHELDFLQAVKDFAGNFTFPKIHNGGIAFINGRHLFIENPQPVSYVVGRKPESFDVPGSEVVKDEKVVILTGANSGGKTSLLELITQITLLAHMGFPVPAEKAWVEPLDELFFFRRKRSAYGAGAFETALRSFVRALRGEGRKLILIDEFEAITEPGAAVKIIGELLKVAHERGFHVVIVSHLGEDLKAELPFARVDGIEAQGLDENLNLIVDRQPAFGKLGRSTPELIVERLARKKRGKEREIFERVLRAFRER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8U0</accession>
    <name>A0A218P8U0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">CRISPR-associated exonuclease Cas4</fullName>
            <ecNumber evidence="2 12">3.1.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">A3L08_07415</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="12">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="12">Mg(2+) or Mn(2+) required for ssDNA cleavage activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>iron-sulfur cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30408"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the CRISPR-associated exonuclease Cas4 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.12.1" evidence="2 12"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8U0"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07415"/>
    <dbReference type="OrthoDB" id="10444at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051827">
        <property type="entry name" value="Cas4_exonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013343">
        <property type="entry name" value="CRISPR-assoc_prot_Cas4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038726">
        <property type="entry name" value="PDDEXK_AddAB-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00372">
        <property type="entry name" value="cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36531">
        <property type="entry name" value="CRISPR-ASSOCIATED EXONUCLEASE CAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36531:SF2">
        <property type="entry name" value="CRISPR-ASSOCIATED EXONUCLEASE CAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12705">
        <property type="entry name" value="PDDEXK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="7 12" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 12" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="11 12" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PD-(D/E)XK endonuclease-like" evidence="13">
        <location>
            <begin position="63"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF12705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ07160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="211" mass="25146" checksum="F0B7CE97E8038520" modified="2017-09-27" version="1">MHDNGSDGDGLLEFYASEALTCPRRIYFRLKGYPERWPEFVKVRLNQGINTHSVLGEILKKRFGFELEKHLVLRSSRLGFEIHGRIDAIGDFPIEIKGKTSLPRMPYDYHMAQLNIYMRWAEAEYGYLYYIKLHEEPTKIISKIDFSQFPIVKGPNFRAFEVPYDKHLFKETLRHFYSVKKAYEREKPPRGQYSYLCKFCPYRYLCYPGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8X2</accession>
    <name>A0A218P8X2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Magnesium transport protein CorA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">corA</name>
        <name evidence="9" type="ORF">A3L08_07770</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Mediates influx of magnesium ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854471.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8X2"/>
    <dbReference type="GeneID" id="33316156"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07770"/>
    <dbReference type="OrthoDB" id="28779at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015087">
        <property type="term" value="F:cobalt ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12828">
        <property type="entry name" value="TmCorA-like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.340:FF:000012">
        <property type="entry name" value="Magnesium transport protein CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.20">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045861">
        <property type="entry name" value="CorA_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004488">
        <property type="entry name" value="Mg/Co-transport_prot_CorA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00383">
        <property type="entry name" value="corA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46494">
        <property type="entry name" value="CORA FAMILY METAL ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46494:SF1">
        <property type="entry name" value="CORA FAMILY METAL ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143865">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="265"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="326" mass="37947" checksum="B8D9C844D75590E6" modified="2017-09-27" version="1">MGEEPEKPRITVIAYSKDRFMSGKLSDIREVLEITGYDVVWVNVDTVSLIPELKEVLGIHEVLVRDFKRGAGRPRVMVFPDYLFVLLHQIYEKDGGLKRERIGLLLKGNLLVTVQEIPGDVFDPIRESIREGEGLFRERGADYLLFALLEAIVENYVPIIERISSRMEELEARILSKGDETVLRRIHSIRQEILFMRRTVFPLLEAFRKLELEGTSFFSEETRPYIEELHGHVMEVLEILEGQRELANSLVELYYSTISMKTNDIIRILTVVSTIFIPLTFITGLYGMNFRYMPELYWRYSYPVVLLAMLVIAVGMLVYFKKKVWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Y4</accession>
    <name>A0A218P8Y4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854492.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Y4"/>
    <dbReference type="GeneID" id="33316181"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07880"/>
    <dbReference type="OrthoDB" id="85689at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="62" mass="6865" checksum="7C44341116F8634E" modified="2017-09-27" version="1">MMFSALVMATFYTLYGFAVDRMGLQKARLAFAVLLPGIGLAFKVMQSISVLGDVEPRHLSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P901</accession>
    <name>A0A218P901_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bacterial Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854501.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P901"/>
    <dbReference type="GeneID" id="33316191"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07930"/>
    <dbReference type="OrthoDB" id="101228at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046878">
        <property type="entry name" value="Big_14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20251">
        <property type="entry name" value="Big_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bacterial Ig-like" evidence="1">
        <location>
            <begin position="36"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="156" mass="17050" checksum="62BA3744893FFC37" modified="2017-09-27" version="1">MNRFLTAFAVLIIVLGIFYVGTNETSKNTSSDTGTAVMRLDKEVYHAGDDLTLTIINTGSETLLVGASYRLYRLENGEWKELDLGFSFTGIGYTIPPGGNWTQVVPLVTHVSDGAGKLEPLPPGKYRITKMVTIERGRCGRGSDEITLSAEFEVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P902</accession>
    <name>A0A218P902_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TNase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07705</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_250638359.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P902"/>
    <dbReference type="GeneID" id="69102013"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07705"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50830">
        <property type="entry name" value="TNASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TNase-like" evidence="1">
        <location>
            <begin position="59"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="166" mass="18224" checksum="F4A10B3CAFE79E70" modified="2017-09-27" version="1">MTPRPRYVVLVLAMVLFSVVASACIGGSPSTTPTENYSTETSVSKVSTFSTSTHNVPADAIKVYVVKVIDGDTIDVAFPNGTVDRVRFLGVDTPETSASRNKANEYDHITDLKCLASWGVAAKFFVRDYIQDKVVYIEFDPIAGRRGYYGRLLAYIYLKNGTDFTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P941</accession>
    <name>A0A218P941_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Rubredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_08245</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Rubredoxin is a small nonheme, iron protein lacking acid-labile sulfide. Its single Fe, chelated to 4 Cys, functions as an electron acceptor and may also stabilize the conformation of the molecule.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8 9">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
        <text evidence="8 9">Binds 1 Fe(3+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the rubredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854557.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P941"/>
    <dbReference type="GeneID" id="33318412"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08245"/>
    <dbReference type="OrthoDB" id="371635at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043448">
        <property type="term" value="P:alkane catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00730">
        <property type="entry name" value="rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000001">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024922">
        <property type="entry name" value="Rubredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024935">
        <property type="entry name" value="Rubredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050526">
        <property type="entry name" value="Rubredoxin_ET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018527">
        <property type="entry name" value="Rubredoxin_Fe_BS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045768">
        <property type="entry name" value="RubredRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47627">
        <property type="entry name" value="RUBREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47627:SF1">
        <property type="entry name" value="RUBREDOXIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00301">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000071">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00163">
        <property type="entry name" value="RUBREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57802">
        <property type="entry name" value="Rubredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00202">
        <property type="entry name" value="RUBREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rubredoxin-like" evidence="10">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00067809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000071-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ07311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="53" mass="6001" checksum="AD791FDACD262B44" modified="2017-09-27" version="1">MAKWKCIVCGYIYDEDEGDPDTGVEPGTKFEDLPDDWVCPLCGAPKDMFEKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P943</accession>
    <name>A0A218P943_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sugar ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_08200</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854548.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P943"/>
    <dbReference type="GeneID" id="33316246"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08200"/>
    <dbReference type="OrthoDB" id="97781at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43744:SF12">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE PROTEIN MG189-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43744">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE PROTEIN MG189-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="74"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="278" mass="31879" checksum="83436468943DC2F0" modified="2017-09-27" version="1">MYRVRIRTKISIKSLLTHAVLWVFVGIILVPILWTFFTSLKTPVEIARGTVLPEVWRWENYKIAWEQAEFPRKFLNSFIVAITVTLGQIVTSAMAGYALARMEFRGRDLIFNVAIASMLISEQIIIVPLYVMLAKFGWIDSYKGLAIPWFFNGFGVFLFRQFFLTIPKDIEEAAIVDGASRFRILWQIMLPLATPAVLTLFMFTFIAEWNSLFKWLIIVKSPEMRNVQLGLTIFQEQFIAQYNLLTAATILVSLPSVILFLIGQRYYIKGIATTGVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P948</accession>
    <name>A0A218P948_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">6-phosphogluconate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_08185</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854545.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P948"/>
    <dbReference type="GeneID" id="33316243"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08185"/>
    <dbReference type="OrthoDB" id="23890at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029154">
        <property type="entry name" value="HIBADH-like_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015815">
        <property type="entry name" value="HIBADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051265">
        <property type="entry name" value="HIBADH-related_NP60_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43580:SF2">
        <property type="entry name" value="CYTOKINE-LIKE NUCLEAR FACTOR N-PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43580">
        <property type="entry name" value="OXIDOREDUCTASE GLYR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14833">
        <property type="entry name" value="NAD_binding_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000103">
        <property type="entry name" value="HIBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase NADP-binding" evidence="3">
        <location>
            <begin position="2"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyisobutyrate dehydrogenase-like NAD-binding" evidence="4">
        <location>
            <begin position="155"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="280" mass="31389" checksum="D19175B956F0FD80" modified="2017-09-27" version="1">MIGWIGLGHIGRAMAERLSEEYELIVWNRTLEKAKGFRNVAKSPEEVVEKADIVFLSLYDSNAVEEVSKRILSKDIKGKIIVDTTTNHHRKVLRFHELYKSAGAHYLESPVIGSVIPARNGQLTILVSGEKEAYEKALPYLEKLGKKIFYFEEPGKATKLKLINNFVLGAFMAALAEAIALGEKAGIPREELVEVLENGAGNSVVLKAKKEKILSEDYSTHFSIKNLVKDLSYAYDLALDANKTVPLNAVVRELYRVAFEKGLEELDFSAIYKIIREFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P967</accession>
    <name>A0A218P967_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P967"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08255"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="201" mass="22085" checksum="5FC84C7212610EC6" modified="2017-09-27" version="1">MNIYFTTMAAGGMLVLLGIFLTWNLARVVEKFRTGKKRLSWLVLLGGLLTAMGFIPAVAMADSSVIVWAVILGPVLISYALSESGLVRANLEMLLQVGVVIASLVLQSGDYIAIAESFSAVSIILLINAVAFYIHTPPGISRTSKAAAWLFAIFVLLNAWGRGNPYVLSIYILSMFLWISALVRLHFIARDRFYRNAQEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9C1</accession>
    <name>A0A218P9C1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Restriction endonuclease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_08665</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_157721608.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9C1"/>
    <dbReference type="REBASE" id="210320">
        <property type="entry name" value="TpaP4McrBCP"/>
    </dbReference>
    <dbReference type="GeneID" id="33316339"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08665"/>
    <dbReference type="OrthoDB" id="62357at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019292">
        <property type="entry name" value="McrC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38733">
        <property type="entry name" value="PROTEIN MCRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38733:SF1">
        <property type="entry name" value="TYPE IV METHYL-DIRECTED RESTRICTION ENZYME ECOKMCRBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10117">
        <property type="entry name" value="McrBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="457" mass="52897" checksum="D91BE33A28EE9728" modified="2017-09-27" version="1">MTVVDLFEFTPINYTTGKKAEFDEDSKTLVLLENQLSKLEKLNEEIKFLEIGRKTIKPRNFVGVVQIDDLTLQIFPKLLRTSSYDDLESHKTLIMGNLLKMLAVGGEIPVKPSEVAGVLSEKAPFLEILISIYSQKLLETLRYHRHYTYRRVEEELNHVKGQIDFGAYASRWHRRHVIPVRYNDRTMDSLLNRTLKYGAYLMARLTQSHENYLRLRSAMEILDGVPLVPVSVYETYRIHFNRLNAVFKPLVEIARMFISGTTLRLQTGRIETFTFLVPMEKVFESFVAGLITNSEYEALPKEFEGSIIKTQHHIGNLLAEGKFWLIPDITIQTTDGMKIIIDTKYKLLDKEDQKLGVSQSDLYQMYAYASHWNADAVVLLYPSISSVINRKWHFNIKTKGTEKKVPLLIKSLDLGSNFLDEGEWEKFLEEFRNLMGEILGEKRQQLEYGEQEALVNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9F6</accession>
    <name>A0A218P9F6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08575</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9F6"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08575"/>
    <dbReference type="OrthoDB" id="11368at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051011">
        <property type="entry name" value="Metal_resp_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43132">
        <property type="entry name" value="ARSENICAL RESISTANCE OPERON REPRESSOR ARSR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43132:SF2">
        <property type="entry name" value="ARSENICAL RESISTANCE OPERON REPRESSOR ARSR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12840">
        <property type="entry name" value="HTH_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="223" mass="24108" checksum="E87ACF58A1268B50" modified="2017-09-27" version="1">MDYETIDIHDERAKELAQILVNDKAIAILHLIEERALSISEISRELNLPISTVSYHIDRMLNVGLVEVAGKKYGKRLQEVKLYRASNKPILLIPRKSMAKVKKKTIMGFEKLHVISLGLAGLVAAGVYAAARGILAPMSGGSPEEVSSSAGNITIMSTPERAIVPTATNTSTSAIFQSTSETLSSASSHPIVWAHPTIMSAGLAVVAFILTFLLVSYILKRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9H2</accession>
    <name>A0A218P9H2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08975</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854686.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9H2"/>
    <dbReference type="GeneID" id="33316401"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08975"/>
    <dbReference type="OrthoDB" id="130870at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30006:SF24">
        <property type="entry name" value="SLL0237 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30006">
        <property type="entry name" value="THIAMINE-BINDING PERIPLASMIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="480" mass="53594" checksum="8BFCCB8A346AC232" modified="2017-09-27" version="1">MKKLASIGVILLLALSVVASGCVSSGGGDETSGITLVVVTRHDATIQYMAKQLFLQSDIAKEYNIEDIKFIKVPESLWPSYIEKGADVGWGGGPTLFDDLYKAGHLAPITDERVLKLIGNPLPTELAGMPMIRKDGDKVYWIAAALSSFGFTVNKKKLAEWNLPIPQKWEDIASEQWALNPPQYGLADPTRSTSNTRIYQIILQAFGWDQGWRIMTLIAANSKIYMQSDAVRDAVINGEIAAGNTIDFYGYTAMQQNPDCLYVVPKGESIINGDPIALLAKAQHPEAAQAFIYWVLTEGQTIWMSPDINRLPINPQVFDMKVTKEYADVVFKGEHVGETYGEARPALKKAYEDAIHAEGIPFDDKRALETINALQYYFKATLVDPNQKLHDAWVAIVQAYKDGKISEEQFNQLKDELTAPIEFKDPDTGKTVTLTEDYAKSINERIMKDRNFQDQLVQEWRQAASDKYDKVLQDLKAITG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9J4</accession>
    <name>A0A218P9J4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF835 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9J4"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09050"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008553">
        <property type="entry name" value="DUF835"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05763">
        <property type="entry name" value="DUF835"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF835" evidence="1">
        <location>
            <begin position="22"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="133" mass="15587" checksum="8662483C899ED108" modified="2017-09-27" version="1">MFWSSGLINDEELWRVLRSNPSRQKIVITRKNNLNDLRNTRTIYLSKVSRPGYFDPSKLYVLEQNLWRHLQNSPSDVILDAFEYLAIENGLETALKFTGKLRDMAVLTGSRFYVTVSDALEERTIHLLRRILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9J5</accession>
    <name>A0A218P9J5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Rhomboid family intramembrane serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_09085</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854705.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9J5"/>
    <dbReference type="GeneID" id="33316423"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09085"/>
    <dbReference type="OrthoDB" id="26567at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050925">
        <property type="entry name" value="Rhomboid_protease_S54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731">
        <property type="entry name" value="RHOMBOID PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731:SF26">
        <property type="entry name" value="RHOMBOID-LIKE PROTEIN 10, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="15"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="58"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="149"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="170"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="6">
        <location>
            <begin position="56"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="203" mass="22230" checksum="7B45F51C46F464EA" modified="2017-09-27" version="1">MGLERYFYRYGKATFSLFLINAAVYVVEAVLSGNPISIGSNVLLLLGQWNYAVLNGAWWQLFTAMFVHVGILHIGFNMYFLLMMGRQLEHILGPKRLVMVYIVSGLMGNLLTLVLLPPNTVSAGASGALFGIAGTLIVMMGVIGGNMQGALVNAFVLFLINSILPGVNPYAHLGGLLVGMVIGYYYGKKIRRRMVLAYGYGYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9R3</accession>
    <name>A0A218P9R3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_09350</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854752.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9R3"/>
    <dbReference type="GeneID" id="33316476"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09350"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="99" mass="11111" checksum="B1B233B3926CC63A" modified="2017-09-27" version="1">MVRISTLGFLMVLASIPLLIHPFGWVLYVGIAFLFIITGFAMMKIGGELESLRGEVEDLHSELMKMRETMRGRGNEARQGSPRSLCRPHRDGDGGLWHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9S9</accession>
    <name>A0A218P9S9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09435</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854767.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9S9"/>
    <dbReference type="GeneID" id="33316493"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09435"/>
    <dbReference type="OrthoDB" id="9937at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050126">
        <property type="entry name" value="Ap4A_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011152">
        <property type="entry name" value="Pesterase_MJ0912"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42850:SF2">
        <property type="entry name" value="BLL5683 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42850">
        <property type="entry name" value="METALLOPHOSPHOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000883">
        <property type="entry name" value="Pesterase_MJ0912"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="235" mass="27111" checksum="B11ECAA370912A9F" modified="2017-09-27" version="1">MIIALISDIHSNWEALQAVWKEIKKADAILCMGDLVGYGASPNEVVEFVREQMEKRTFLCVRGNHDNAVAFGLDWGFNPYAREAVRWHQRVMSIENLEFLRRLPVRQLFTDDRGRSYLLIHGSPRAPLDEYLFPWLPDSEFKAVLSYIKQDDLLVGHTHVPMLKLLGGRRIINPGSVGQPRDGKWRAAYAIMDTEEEPPDNVEFHRVEYDVEEAARKILEAGLPRFLAERLYDGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9T2</accession>
    <name>A0A218P9T2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_09480</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854776.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9T2"/>
    <dbReference type="GeneID" id="33316502"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09480"/>
    <dbReference type="OrthoDB" id="30785at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.60">
        <property type="entry name" value="YhbY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001890">
        <property type="entry name" value="RNA-binding_CRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051925">
        <property type="entry name" value="RNA-binding_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035920">
        <property type="entry name" value="YhbY-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40065">
        <property type="entry name" value="RNA-BINDING PROTEIN YHBY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40065:SF3">
        <property type="entry name" value="RNA-BINDING PROTEIN YHBY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01985">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01103">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75471">
        <property type="entry name" value="YhbY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51295">
        <property type="entry name" value="CRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CRM" evidence="3">
        <location>
            <begin position="3"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="138" mass="16323" checksum="ECBDE636C62B1F89" modified="2017-09-27" version="1">MEKRLSGKVRRAIRARYYDIPPRAWIGKRGLDESVIEEINTQLEKDGILKVEIRKGALISTGLDRGTLARKVAEMTDSELIDVRGKRFILFKPREGWEKYLRKLQRKELSKEKREEKPVKKVKLDIAQFRRKFKKGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9T7</accession>
    <name>A0A218P9T7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09485</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854777.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9T7"/>
    <dbReference type="GeneID" id="33316503"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09485"/>
    <dbReference type="OrthoDB" id="108920at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055834">
        <property type="entry name" value="DUF7411"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011155">
        <property type="entry name" value="PRK14561.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24167">
        <property type="entry name" value="DUF7411"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="205" mass="23763" checksum="135E10251DD84A8E" modified="2017-09-27" version="1">MLVHHLYSGGKDSSLAAWILTRLGYNVELVTVSFGLLDNWRFARETAERLGFEHRVLYLPKETLEKAAEMAIRDGHPNNAIQFIHEKALEALASLPEVERISDGTRRDDRVPLLDLPRARSLEDRFGVAYIRPLLGLGYKTIRELTERLFVVEIRESEELEKADYEVELRHLLREKGIDPLGIFPKSHYQSRVMGWKEGRNREIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9V1</accession>
    <name>A0A218P9V1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09560</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854791.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9V1"/>
    <dbReference type="GeneID" id="33316518"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09560"/>
    <dbReference type="OrthoDB" id="94367at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="275" mass="31354" checksum="EA2A7B1452EC7DCD" modified="2017-09-27" version="1">MRFVTKNEAVKRSFLEDLKREGIKFELNERLSYESFVGYMLEGTLEEIRAQIEVMEGADKEALIEGFTSFKESLNHILEHIKVGEKFEVLLNEGPWVAEILDQLTRNGAIDYEDGVIKLRDGVDVTKLRFEFKFPFNLVHNPESAEKIAKQFAFADLTMEYEFEILELDMGKINALGKIASRYFPEDVLLRVYFALVGRAILSSEILKSLGNEKVELSELVKAFARAAPIAIPTPKGTLVINYSPVALEEVLRFLKRLGYIEIKAGKVKKLRDLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9V5</accession>
    <name>A0A218P9V5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MFS transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854809.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9V5"/>
    <dbReference type="GeneID" id="33316538"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09660"/>
    <dbReference type="OrthoDB" id="94818at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23518">
        <property type="entry name" value="C-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23518:SF2">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="365"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="393" mass="44357" checksum="C0A0A60FC880C2F7" modified="2017-09-27" version="1">MRWSEIPRDAKAYMFYHTLIAPGLIVWILFPLYLMETGYSILEVGAFFTAVNLTSIPLTYIFGRAFNRWDMKKGLIAIDVLDGIAYVLYGLAKGALAPVLLFAGRAIEKLSTMLYPLYRAYEQIIYPEDKYEEIFAWHLRLPEIARVLTFPVMGYILGYVYPGPESYRWAFIIFGLFSAVTVAYLWLFLPSIGKEERITPEGFTFKVGEFKLLLAFEALLTLAWALAPEMVLINYVVFTLHKTVFEVTLVAVASSVASILGTYASERVPKGKGFQAMALGMLLNAFYALIMALSPPFWIVLAVYALGDFGNTFWFPFYRAWMFKLIPKERASEFHAAISSYRKVIGIVAPFIAGALASIHATLPYAVSFGLFLLAGVMFWWPARKGIYSRTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Z0</accession>
    <name>A0A218P9Z0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09815</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854838.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Z0"/>
    <dbReference type="GeneID" id="33316569"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09815"/>
    <dbReference type="OrthoDB" id="84946at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00880">
        <property type="entry name" value="Era_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42714">
        <property type="entry name" value="TRNA MODIFICATION GTPASE GTPBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42714:SF2">
        <property type="entry name" value="TRNA MODIFICATION GTPASE GTPBP3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="1">
        <location>
            <begin position="3"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="185" mass="20891" checksum="2CFF745B52054AC6" modified="2017-09-27" version="1">MIKVAIIGAENAGKSTLMNALIGGKVSEVEDLPGTTKGVIRRRFGKLKIPKGMKNPLGGADEFVLIDTAGLFDPHRELRGKVLSEERFKDIINEIVSADIIIHMVDATVGLHRGMEKLHHMLKFRYEKPIIVVINKIDLVPRERVEELREIIKKRLEQDALPLSLVTYEGFNELLEKLAHYAQYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Z6</accession>
    <name>A0A218P9Z6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">5'-deoxynucleotidase</fullName>
            <ecNumber evidence="5">3.1.3.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_09805</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-phosphate + H2O = a 2'-deoxyribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:36167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18274"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:65317"/>
            <dbReference type="EC" id="3.1.3.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.89" evidence="5"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854836.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Z6"/>
    <dbReference type="GeneID" id="33316567"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09805"/>
    <dbReference type="OrthoDB" id="46088at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002953">
        <property type="term" value="F:5'-deoxynucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3210.10:FF:000035">
        <property type="entry name" value="HD family hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039356">
        <property type="entry name" value="YfbR/HDDC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845:SF13">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13023">
        <property type="entry name" value="HD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD/PDEase" evidence="8">
        <location>
            <begin position="30"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="188" mass="21678" checksum="F606FDBD21D9D5AC" modified="2017-09-27" version="1">MVVSLLELFLEAGNLKRLPRTGWLLRGVPVPESIADHSFRTALMVLFLADELKAKGVEINTEKAIRMAILHDLAETRITDVPLTAQRYLDKGKGEREAFMEMLSSVGRRDLLSLFGEYENETSIEGRLVKFADRLEMLIQAYEYEKAGFGDLDEFWGAVEKLRENELYMHFKDLLDGLVELRKQKRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P4Z0</accession>
    <name>A0A218P4Z0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribulose bisphosphate carboxylase</fullName>
            <shortName evidence="6">RuBisCO</shortName>
            <ecNumber evidence="6">4.1.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rbcL</name>
        <name evidence="9" type="ORF">A3L08_00080</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the addition of molecular CO(2) and H(2)O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2 (2R)-3-phosphoglycerate + 2 H(+) = D-ribulose 1,5-bisphosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57870"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="EC" id="4.1.1.39"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>D-ribulose 1,5-bisphosphate + O2 = 2-phosphoglycolate + (2R)-3-phosphoglycerate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:36631"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57870"/>
            <dbReference type="ChEBI" id="CHEBI:58033"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="6">Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O(2) to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the RuBisCO large chain family. Type III subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.39" evidence="6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853104.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P4Z0"/>
    <dbReference type="GeneID" id="33314620"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00080"/>
    <dbReference type="OrthoDB" id="52787at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016984">
        <property type="term" value="F:ribulose-bisphosphate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006196">
        <property type="term" value="P:AMP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015977">
        <property type="term" value="P:carbon fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08213">
        <property type="entry name" value="RuBisCO_large_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.110">
        <property type="entry name" value="Ribulose bisphosphate carboxylase, large subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.150">
        <property type="entry name" value="RuBisCO large subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01133">
        <property type="entry name" value="RuBisCO_L_type3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033966">
        <property type="entry name" value="RuBisCO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017712">
        <property type="entry name" value="RuBisCO_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000685">
        <property type="entry name" value="RuBisCO_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036376">
        <property type="entry name" value="RuBisCO_lsu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017443">
        <property type="entry name" value="RuBisCO_lsu_fd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036422">
        <property type="entry name" value="RuBisCO_lsu_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003252">
        <property type="entry name" value="PRK04208.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03326">
        <property type="entry name" value="rubisco_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42704">
        <property type="entry name" value="RIBULOSE BISPHOSPHATE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42704:SF17">
        <property type="entry name" value="RIBULOSE BISPHOSPHATE CARBOXYLASE LARGE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00016">
        <property type="entry name" value="RuBisCO_large"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02788">
        <property type="entry name" value="RuBisCO_large_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01052">
        <property type="entry name" value="RuBisCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00014">
        <property type="entry name" value="RuBisCO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00301">
        <property type="entry name" value="RuBisCO-like_proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51649">
        <property type="entry name" value="RuBisCo, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54966">
        <property type="entry name" value="RuBisCO, large subunit, small (N-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0120">Carbon dioxide fixation</keyword>
    <keyword evidence="4 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribulose bisphosphate carboxylase large subunit ferrodoxin-like N-terminal" evidence="8">
        <location>
            <begin position="12"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Ribulose bisphosphate carboxylase large subunit C-terminal" evidence="7">
        <location>
            <begin position="142"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="6">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="367"/>
            <end position="369"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="389"/>
            <end position="392"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="6">
        <location>
            <position position="322"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="6">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ05841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="444" mass="50401" checksum="BCF222D0B0ED507F" modified="2017-09-27" version="1">MVEKFDKIYDYYVDKGYEPNKKRDIIAVFRVTPAEGYTIEQAAGAVAAESSTGTWTTLYPWYEHERWADMSAKAYHFTDMGDGSWIVRIAYPEHIFEEGNMPGFLASVAGNVFGMKRVEWLRLEDIYLPERFLRNYPGPNFGIEGVRKKLEIYDRPLYGVVPKPKVGYSPEELYKLALDLYTGGADYLKDDENLTSPWYNRFEERVKVVTKAMEKAENETGEKKTWFANITSPILEMEQRLEILADYGVPHAMVDVVVLGWGVLDYIRELAEDYGIALHAHRAMHTAFARYKYHGISMFVLAKLYRVIGVDQLHIGTAGAGKMEGEKWEVIQYKRIITEDHYVPDENDVYHLEQKFYHIKPVFPTSSGGLHPGNIQPVIDALGKDIVLQLGGGTMGHPDGPAAGARAVRQAIDAIMQGIPLDEYAKTHKELARALEKWGHVTPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P4Z8</accession>
    <name>A0A218P4Z8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Diphthamide synthase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853114.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P4Z8"/>
    <dbReference type="GeneID" id="33314630"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00130"/>
    <dbReference type="OrthoDB" id="372052at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017178">
        <property type="term" value="F:diphthine-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01994">
        <property type="entry name" value="AANH_PF0828-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1490.10:FF:000006">
        <property type="entry name" value="ATP binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000145">
        <property type="entry name" value="ATP-binding domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1490.10">
        <property type="entry name" value="putative n-type atp pyrophosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002761">
        <property type="entry name" value="Diphthami_syn_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030662">
        <property type="entry name" value="DPH6/MJ0570"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005237">
        <property type="entry name" value="MJ0570"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022427">
        <property type="entry name" value="MJ0570_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03679">
        <property type="entry name" value="arCOG00187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00290">
        <property type="entry name" value="MJ0570_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00289">
        <property type="entry name" value="TIGR00289 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196:SF2">
        <property type="entry name" value="DIPHTHINE--AMMONIA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196">
        <property type="entry name" value="DOMAIN OF UNKNOWN FUNCTION 71 DUF71 -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01902">
        <property type="entry name" value="Diphthami_syn_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039123">
        <property type="entry name" value="Diphthamide_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Diphthamide synthase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="225" mass="25762" checksum="3C2EA4FCD9EB6798" modified="2017-09-27" version="1">MRVAVLYSGGKDSNYALYWALKQGFEVKYLVSMVSESDESYMYHVPNIHLTELQAKAIGIPLVKGFTSGEKEKEVEDMKAVLEGLKIDGVVAGALASEYQKRRVDRVVEELGLKSFAPAWHRDPVEYMRELVEIFDIVMVGTAAYGLDESWLGRKIDEKTLEELVKLNEKYRVHVAGEGGEFETFVRDAPFFKARIVFDEVEKKWNECNYSGILEVRRAHLERKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P517</accession>
    <name>A0A218P517_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0201 protein A3L08_00255</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0201 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853138.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P517"/>
    <dbReference type="GeneID" id="33314655"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00255"/>
    <dbReference type="OrthoDB" id="7819at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01112">
        <property type="entry name" value="UPF0201"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002739">
        <property type="entry name" value="PAB1135-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001687">
        <property type="entry name" value="PRK00447.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39652">
        <property type="entry name" value="UPF0201 PROTEIN TK1335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39652:SF1">
        <property type="entry name" value="UPF0201 PROTEIN TK1335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01877">
        <property type="entry name" value="RNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="137" mass="15718" checksum="45B948BE336621AE" modified="2017-09-27" version="1">MELFEEVEVEAYVYPTEDIEKVKKAMLNLIPDLEFEAFDEGDYIILTGKTRSRKALSRLYELFRGQAILDTARSFLEEGYFGEEIIIKVNKQAAYAGVVNFNEESPLGPITIIIRTKNPKKLMKWLAPRTKDGVPIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P555</accession>
    <name>A0A218P555_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_00420</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P555"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00420"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ05905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="83" mass="9548" checksum="F7846A2F7D07B14E" modified="2017-09-27" version="1">MRFLGTEEIWCLLGGREEKSGSIGPGTRVRKILKNFPQRKGFGILWMVVRQAGDLEYVEVWVIKGKTRYNLLFQKISPETTCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P556</accession>
    <name>A0A218P556_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cation:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_00395</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853164.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P556"/>
    <dbReference type="GeneID" id="33314683"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00395"/>
    <dbReference type="OrthoDB" id="18006at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050601">
        <property type="entry name" value="CPA3_antiporter_subunitC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039428">
        <property type="entry name" value="NUOK/Mnh_C1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005623">
        <property type="entry name" value="PRK07375.2-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34583">
        <property type="entry name" value="ANTIPORTER SUBUNIT MNHC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34583:SF3">
        <property type="entry name" value="MULTISUBUNIT SODIUM_HYDROGEN ANTIPORTER, MNHC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00420">
        <property type="entry name" value="Oxidored_q2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ05901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="99" mass="10506" checksum="4B9AA2B5E1324ED0" modified="2017-09-27" version="1">MISPEQAGIIIMLVGIYGLMTKREPVKLVLSINIVSLGLVLFFVGLAYSPGKDVPIMPTDPVDPLPATLMLTTLVVDVAITSLALAIIIRMRGDGECSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P557</accession>
    <name>A0A218P557_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_00430</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853170.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P557"/>
    <dbReference type="GeneID" id="33314690"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00430"/>
    <dbReference type="OrthoDB" id="102388at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ05907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="216" mass="25264" checksum="9BE511391B68ED56" modified="2017-09-27" version="1">MGKDEVLVEIDRRIKRLEAEISMAEERMRYLEEIGAPVKYRALQRKDYTVYYLILMGVWIVIGMLALLLMKNRLPYYFNVPLMPYFIIALVLLVAPAVYLIWSRRESPPTPMEDLEERERLSRVVLNLFYRPLREAVEENDMEKMRALADELLSNPVLASGVERMAEGDPKLNAYALYLYASYTPELESEVRDTIEKLTNRPLKVLLSGLLEKERD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P570</accession>
    <name>A0A218P570_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TATA-box-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Box A-binding protein</fullName>
            <shortName evidence="7">BAP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">TATA sequence-binding protein</fullName>
            <shortName evidence="7">TBP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">TATA-box factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tbp</name>
        <name evidence="9" type="ORF">A3L08_00485</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7 8">General factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Binds specifically to the TATA box promoter element which lies close to the position of transcription initiation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the TBP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853180.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P570"/>
    <dbReference type="GeneID" id="33314701"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00485"/>
    <dbReference type="OrthoDB" id="350539at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04518">
        <property type="entry name" value="TBP_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000007">
        <property type="entry name" value="TATA-box-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000010">
        <property type="entry name" value="TATA-box-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00408">
        <property type="entry name" value="TATA_bind_prot_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033711">
        <property type="entry name" value="TBP_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030491">
        <property type="entry name" value="TBP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001593">
        <property type="entry name" value="PRK00394.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001594">
        <property type="entry name" value="PRK00394.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00686">
        <property type="entry name" value="TIFACTORIID"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00351">
        <property type="entry name" value="TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="repeat" description="1" evidence="7">
        <location>
            <begin position="11"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="repeat" description="2" evidence="7">
        <location>
            <begin position="102"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ05917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="190" mass="21406" checksum="9E8D39985A12EB8C" modified="2017-09-27" version="1">MVDMSNVKLRIENIVASVDLFAQLNLEKVIEICPNSKYNPEEFPGIICRFEEPKVALLIFSSGKLVVTGAKSVEDIERAVNKLIQMLKKIGAKFARAPQIDIQNMVFSGDIGMEFNLDAVALSLPNCEYEPEQFPGVIYRVKEPRAVILLFSSGKIVCSGAKSEHDAWEAVRKLLRELEKYGLIEEEDEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P574</accession>
    <name>A0A218P574_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acetyl-CoA acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_00525</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853188.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P574"/>
    <dbReference type="GeneID" id="33314709"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00525"/>
    <dbReference type="OrthoDB" id="167534at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00829">
        <property type="entry name" value="SCP-x_thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055140">
        <property type="entry name" value="Thiolase_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004720">
        <property type="entry name" value="PRK06064.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870">
        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870:SF6">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22691">
        <property type="entry name" value="Thiolase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="2">
        <location>
            <begin position="5"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="3">
        <location>
            <begin position="244"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ05925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="388" mass="41135" checksum="1919481C184861B5" modified="2017-09-27" version="1">MEKPVIIGVGMVPVGEHWRVSLRDMAVEALLNAMEDAGIDKVDSLYVGNMASGSFIEQENLGALIADWAGLGNIPAVKVEAACGSGGAAVQEGAKAVMSGLEDVVAVVGVEKMTDAWPSDATRFLGYAADAEWELFHGASFVALNALIMRLYMKTYGYTEEDLAHFAVNAHRNGAKNPYAMFKKEIKLETVLNSPYVADPIKLFDAAPMCDGAAAVIITSKEKAKELGVPKEGMVELAGFWRAIDTINLANREDFLTLKAAKVAAEKAYKMAGVTAKDIDFFEVHDAFTVMAALSLEALGVAEKGKGAMLAKEGQIAIDGDYPIQTMGGLKARGHPVGATGVYQTVEAVLQLRGEAPEGIQVPDAEVGLTQNIGGTGSNITVNILRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P586</accession>
    <name>A0A218P586_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(2Fe-2S)-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_00675</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853215.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P586"/>
    <dbReference type="GeneID" id="33314739"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00675"/>
    <dbReference type="OrthoDB" id="36306at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19946">
        <property type="entry name" value="GlpA-like_Fer2_BFD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1100">
        <property type="entry name" value="BFD-like [2Fe-2S]-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041854">
        <property type="entry name" value="BFD-like_2Fe2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051691">
        <property type="entry name" value="Metab_Enz_Cyan_OpOx_G3PDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041117">
        <property type="entry name" value="SoxA_A3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42949">
        <property type="entry name" value="ANAEROBIC GLYCEROL-3-PHOSPHATE DEHYDROGENASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42949:SF3">
        <property type="entry name" value="ANAEROBIC GLYCEROL-3-PHOSPHATE DEHYDROGENASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17806">
        <property type="entry name" value="SO_alpha_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SoxA A3" evidence="2">
        <location>
            <begin position="4"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ05953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="90" mass="9906" checksum="580EA48E541CC06C" modified="2017-09-27" version="1">MSGKVIICRCNDVTVEEIEALIDEGVTDIEEIKRLLRIGMGPCQGRTCIPLVMAILARKTGKKPDEIPLPRARVPIRPVRVEVLVGGDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5A3</accession>
    <name>A0A218P5A3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dihydropyrimidine dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_00760</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853228.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5A3"/>
    <dbReference type="GeneID" id="33314756"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00760"/>
    <dbReference type="OrthoDB" id="27922at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01653">
        <property type="entry name" value="GATase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028261">
        <property type="entry name" value="DPD_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006004">
        <property type="entry name" value="SudA-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01316">
        <property type="entry name" value="gltA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42783">
        <property type="entry name" value="GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42783:SF3">
        <property type="entry name" value="GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14691">
        <property type="entry name" value="Fer4_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydroprymidine dehydrogenase" evidence="2">
        <location>
            <begin position="23"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="159"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ05966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="482" mass="52940" checksum="FFF08F796C96681E" modified="2017-09-27" version="1">MVRKRPKLIKERVPTPERPVEERVKSFVEVNLGYDFASAVKEAERCLQCPVEYAPCIKGCPVHINIPGFIGKLVEHRDDPNKAVKEALRVIWGDNTLPAVTGRVCPQEEQCEMNCVMGKVGDPINIGKLERFVADYARKHSIEDELLAEMRPSVCGKGKVAVVGAGPAGLTCAGELAKLGYDVTIYEALHKPGGVLIYGIPEFRLPKEILDKEIAKLRELGVEIKLDHVVGKTVTLEELLEEYDAVFIGTGAGTPKLLNIPGILLDRIYSANEFLTRVNLMKAYKFPEYDEPIAVGKKTIVIGAGNTAMDAARSALRLGSEVTIAYRRGREDMTARIEEIQHAEEEGVKFEFFLNPVEFIGDENGRVKAVKFEKMKPLEERDKKGKRKIVGTGEYVTLEADTVIIAIGLEPNRIISEEATGLKVNPDGTLVVDENLMTSIPGVFAGGDAIRGEATVILAMGDGKRAAKAICDYIEKKRKANA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5B9</accession>
    <name>A0A218P5B9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ring hydroxylating protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853222.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5B9"/>
    <dbReference type="GeneID" id="33314750"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00730"/>
    <dbReference type="OrthoDB" id="371709at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052339">
        <property type="entry name" value="Fe-S_Maturation_MIP18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42831">
        <property type="entry name" value="FE-S PROTEIN MATURATION AUXILIARY FACTOR YITW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42831:SF1">
        <property type="entry name" value="FE-S PROTEIN MATURATION AUXILIARY FACTOR YITW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIP18 family-like" evidence="1">
        <location>
            <begin position="83"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="178" mass="20232" checksum="65D921667658427F" modified="2017-09-27" version="1">MVTKEDVERVVKSIVDEKFVKSIEVDDKGNVTVTLARDTPDIDNVLIKLHSEIGKLKGVGLITINREREEKPGEDENVQLTKELILEKLKEVIDPEIGIDVVNLGLIYELEIRPDNTVYVKMTMTTPGCPLTMWILRAVEDKILEIPGVKDAEIELTFDPPWSPDMVSEEYKKRLGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5G8</accession>
    <name>A0A218P5G8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Proteasome subunit beta</fullName>
            <ecNumber evidence="9">3.4.25.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">20S proteasome beta subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Proteasome core protein PsmB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">psmB</name>
        <name evidence="11" type="ORF">A3L08_01010</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.25.1" evidence="9"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853272.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5G8"/>
    <dbReference type="GeneID" id="33314806"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01010"/>
    <dbReference type="OrthoDB" id="6330at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03764">
        <property type="entry name" value="proteasome_beta_archeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000049">
        <property type="entry name" value="Proteasome subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02113_A">
        <property type="entry name" value="Proteasome_B_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019983">
        <property type="entry name" value="Pept_T1A_Psome_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03634">
        <property type="entry name" value="arc_protsome_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF0">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE SUBUNIT HSLV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0645">Protease</keyword>
    <keyword evidence="7 9 11" id="KW-0647">Proteasome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0888">Threonine protease</keyword>
    <keyword evidence="8 9" id="KW-0865">Zymogen</keyword>
    <feature type="propeptide" id="PRO_5011123350" description="Removed in mature form; by autocatalysis" evidence="9">
        <location>
            <begin position="1"/>
            <end position="7"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023247573" description="Proteasome subunit beta" evidence="9">
        <location>
            <begin position="8"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9 10">
        <location>
            <position position="8"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="200" mass="21943" checksum="A659C66D3F0A0239" modified="2017-09-27" version="1">METKKTGTTTVGIKARDGVVLAADTQASLDHMVETLNIRKIVPITDRIAITTAGSVGDVQALARMLEAEARYYQFTWGRPMSTKAMANLLSNILNENKWFPYMVQIIIGGYVDEPTLANLDPLGGLIFDDYTATGSGSPFAIAVLEDGFRKDMSIDEAKELAIRAVRTAGKRDVYTGSRKVQVVVITRDGMKEEFVEFGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5L4</accession>
    <name>A0A218P5L4_9EURY</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853336.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5L4"/>
    <dbReference type="GeneID" id="33314877"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01360"/>
    <dbReference type="OrthoDB" id="84424at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="95" mass="11203" checksum="FCBFF1EE3E220314" modified="2017-09-27" version="1">MEIIIDNFKPKITRPFKRKSEYWVKLILSDGEEYIMKFKSPLEAEDAIYGMLDDLQVYGGKVKLKLREGNVIEHIEVLELYKPSAKELLSEYFTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5N3</accession>
    <name>A0A218P5N3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sodium:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_01520</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
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            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853363.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5N3"/>
    <dbReference type="GeneID" id="33314909"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01520"/>
    <dbReference type="OrthoDB" id="76879at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018461">
        <property type="entry name" value="Na/H_Antiport_NhaC-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43478">
        <property type="entry name" value="NA+/H+ ANTIPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43478:SF1">
        <property type="entry name" value="NA+_H+ ANTIPORTER NHAC-LIKE C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03553">
        <property type="entry name" value="Na_H_antiporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="321"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="488"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="511"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Na+/H+ antiporter NhaC-like C-terminal" evidence="7">
        <location>
            <begin position="156"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="552" mass="60121" checksum="11BE333E5E380848" modified="2017-09-27" version="1">MSDFGILSLLPPLVAIILAIWTKRVILALFAGVWIGGLMVAGWNPITGTTQSLEWVVGSVTDDWNARILVFDFLIGAGVGLVYKSGGVHALARALSRRVKTSRGASVLGWLMGVLVFFDDYTNTIIVGNTMRPITDRTRVSREMLAYIDDSTAAPVAGLALISTWIGYELAMIGRGFDGAEVTYNSYDAWLSSVPFRFYSILAIILVFIVAYTHRHYGPMLKAEYRARTTGKVLRDGAKPLMTTEVDLGMPKEGGSLWDFVIPILSLVVVSLLGLWYTGAANLYAYSQNLDWWTDLENPFGVNFLNYSFVDSFREADAATALLWGSFTMVLVASIILLGKKKMTVEEWEDTVIRGMKQMLFANTVLVLAWSLGTAAETVGTGTYIINLATGSGANLGPWMPLIMFLAAMFVAFTTGTSWGTFAIMVPLGVQLGLAFTNGHVNEVVFATIGATFTGSIFGDHCSPISDTTIMSSMFSGSDHIDHVTTQIPYALTAASIGAVLYVLFGLGVRSWVILLPLGIVLLVAAWYVLSEWYGKKYGILHGKVPIYVVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Q8</accession>
    <name>A0A218P5Q8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5Q8"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01600"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="78" mass="8867" checksum="43B46F1B3C04A69B" modified="2017-09-27" version="1">MTVITRQDVQALLSTICRIQFAKREEVSKRIANKITLGLLIMATSGLRVYELTKLPLSYIDKGSLECPPRFRKQASQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5T2</accession>
    <name>A0A218P5T2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cation:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_01735</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853400.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5T2"/>
    <dbReference type="GeneID" id="33314952"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01735"/>
    <dbReference type="OrthoDB" id="85180at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002758">
        <property type="entry name" value="Cation_antiport_E"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006245">
        <property type="entry name" value="PRK08383.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34584">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER SUBUNIT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34584:SF1">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER SUBUNIT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01899">
        <property type="entry name" value="MNHE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019239">
        <property type="entry name" value="MrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="167" mass="18617" checksum="43F395DA1E630255" modified="2017-09-27" version="1">MPFIVAFVLSYLLWLVLTAGTNGLLWSTQELIAGLIFSLIVAYATRDVIGEKAGRFLNPAKWIGFIAYSPVLFWGMVKANLQVAWLVITGKIRPGIVRVPVDLENDAQYTILSNSITLTPGTLTIEACPEEKALYVHWIKIPEGMERLESSEPVAGPFEKWARRLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5T6</accession>
    <name>A0A218P5T6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01595</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853376.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5T6"/>
    <dbReference type="GeneID" id="33314924"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01595"/>
    <dbReference type="OrthoDB" id="98570at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="85" mass="10207" checksum="FA93251D192FDE27" modified="2017-09-27" version="1">MRKRLFMFVTPDGFTYSSPHVLSPDVDNFQVLGFGEGKDKKEAFRDFLRNNRWVLDTNFNEVISVEVKRRIHEGQFFYLKDYRIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P602</accession>
    <name>A0A218P602_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acylpyruvase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_02130</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAH family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853473.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P602"/>
    <dbReference type="GeneID" id="33315031"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02130"/>
    <dbReference type="OrthoDB" id="6242at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018773">
        <property type="term" value="F:acetylpyruvate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.850.10:FF:000003">
        <property type="entry name" value="Fumarylacetoacetate hydrolase domain-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820">
        <property type="entry name" value="ACYLPYRUVASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820:SF7">
        <property type="entry name" value="ACYLPYRUVASE FAHD1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="3">
        <location>
            <begin position="17"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="225" mass="25109" checksum="AD0366001A5A77F8" modified="2017-09-27" version="1">MVRLPFRDTYYELRPSKIVALAKNYAEHAKEMESDVPERPVFFLKPPSALIGPGEPIILPRMSKRVDHEVELAVIIGKRAKRVPAEKAMDYVLGYTIMLDITARDLQAEAKKKGLPWTISKGFDTFAPVGPRVVDRRELNITDLEIGLKVNGQLRQLGRTSQMVFKVPELIEYISSVMTLEPGDIIATGTPAGIGPLRHGDRVEAWIEGIGMIEFDVLAEDSILC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P618</accession>
    <name>A0A218P618_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Magnesium chelatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_02215</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853489.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P618"/>
    <dbReference type="GeneID" id="33315048"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02215"/>
    <dbReference type="OrthoDB" id="24581at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000640">
        <property type="entry name" value="MoxR family ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.80">
        <property type="entry name" value="Magnesium chelatase subunit I, C-Terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011703">
        <property type="entry name" value="ATPase_AAA-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050764">
        <property type="entry name" value="CbbQ/NirQ/NorQ/GpvN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041628">
        <property type="entry name" value="ChlI/MoxR_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42759:SF5">
        <property type="entry name" value="METHANOL DEHYDROGENASE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42759">
        <property type="entry name" value="MOXR FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07726">
        <property type="entry name" value="AAA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17863">
        <property type="entry name" value="AAA_lid_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002849">
        <property type="entry name" value="AAA_ATPase_chaperone_MoxR_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase AAA-3" evidence="3">
        <location>
            <begin position="40"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="ChlI/MoxR AAA lid" evidence="4">
        <location>
            <begin position="239"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="317" mass="35585" checksum="9065D7C8FA4E138A" modified="2017-09-27" version="1">MKIEEIHEKGNAVLNEVGKAIVGKEEVLKLILTTILADGHVLLEDLPGLAKTLMAKSFAKALGVQFTRVQFTPDLLPSDILGVSVFNQKTLEFEFRKGPVFTNVLLADEINRAPPKTQSALLEAMQERQVTVEGNTYVLPKPFIVIATQNPIEQEGTYPLPEAQLDRFLVRLRVGYPTKAEEIEILRRRMARKKEDVDIQTILSAEQVVEMQRAIEDVYVSDAILEYITDIVTATREDKREIEIGASPRGSLALLKLSRAYAALEGRDYVIPDDVKAVAVPALSHRLILKRELWYTKVSQESIMEKLLGKVPVPKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P626</accession>
    <name>A0A218P626_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF515 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_02170</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_232461745.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P626"/>
    <dbReference type="GeneID" id="33315039"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02170"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007509">
        <property type="entry name" value="DUF515"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04415">
        <property type="entry name" value="DUF515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="349"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="380"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="481" mass="53959" checksum="F19927EC09AA91F8" modified="2017-09-27" version="1">MSEDIETKIRRLRELGKTSAEPEAPKTAVPPVRKPPRKPRTVGSLRERERRKRILTGVAVLLIVILIISVGAYVYIQNRAAKQLTEEKNKKLREVYTYFKGDIVNSSRNCTFGPIEVRNELINRINAAQSLDEIRAIDVKAFYDQAVSDYKECLAKIESMKYEKVLNQTKAEKIRAIETEFQSILAMPLPDDLRTKVVNSMKSLEEQVMSAETIDQVNSVNAAPYLLELWRDYYYYRIDTIPGQEVILERDNVKRIVSKSDAKAILGGVLDYKELMEYNVYKVEYVDIALVLTRDRINGAFLAPGDKVMVFAKNSTNAPFMEIANEGYVQLVLLPTDAGVISVDEAQSQSSSSSVSSSTQYSEDHSTTYNPGDLTISDGQSTSDTYTNTQSSSQSASASYSYSVDLAEILKAIAAGKIQASDEVREQLRAYGWEIVDLEKESGMLVLKPDAQFLVVIRVPSIFVPDILSNQQYLYIAKIAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P632</accession>
    <name>A0A218P632_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_02370</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853516.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P632"/>
    <dbReference type="GeneID" id="33315079"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02370"/>
    <dbReference type="OrthoDB" id="8907at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1350:FF:000021">
        <property type="entry name" value="Cation diffusion facilitator family transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000006">
        <property type="entry name" value="Divalent cation efflux transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="entry name" value="Cation efflux protein, cytoplasmic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050291">
        <property type="entry name" value="CDF_Transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840:SF15">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="Cation efflux protein cytoplasmic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="8">
        <location>
            <begin position="8"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein cytoplasmic" evidence="9">
        <location>
            <begin position="207"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="286" mass="31482" checksum="7B1E36A6E5F03F39" modified="2017-09-27" version="1">MEEVYKPIWLSIVGNVLLALLKLVIGFLYSSIALISDGVHSLSDVITSVIGYAGIRISSKPPDQSHPFGHSRFEPLVAFLIGEALLIGAYEIGRDSVYRLLRGEVIDVNSIMLGVTFLSILAKEAMFRYSVHVGRKLNSQILIADAYHHRSDSLSSLAVLVGLGAQRLGFRYGDALAGLVVAVFLMKVSLDVILQNIGYLTGQAPSFEVCEEIKKRALGVPNVLGVHDLRAHYVGNKLHVELHIEVPPELTLKEAHDISEEVKKRVEEIPEVDRTFVHVDIKGITE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P658</accession>
    <name>A0A218P658_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Sodium:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">A3L08_02435</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853527.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P658"/>
    <dbReference type="GeneID" id="33315092"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02435"/>
    <dbReference type="OrthoDB" id="12029at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562">
        <property type="entry name" value="NAPA-TYPE SODIUM/HYDROGEN ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562:SF3">
        <property type="entry name" value="SODIUM ION_PROTON EXCHANGER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="212"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="259"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="287"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="333"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="11">
        <location>
            <begin position="13"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="374" mass="40373" checksum="DDACF5FF183819C4" modified="2017-09-27" version="1">MEIILLIALMLAMAKIMGYVFERLGQPVVLGQLIGGFIMGIFFNTEPVIQEFSNLGVLMLLFLAGLESELEEFKRVGKPSVMVAGVGVLVAFAFGFLAAYPFVEPHEAVLYGAIMTPTSVSITVKVLMELRKLNTREGTTILAAAVVDDVLGILILTVAISLLSEGSVHYSVLAEIVAEVSAFLFVFLYFGPKFADRAFRIISRIDLPETSTSFAIIFMIVFAVLAEHLNLASILGAYLTGLALGQSSRKREIVEHVSTLGYSLFIPLFFVEVGMRVELGYILHAGFFAVVYTLMAVASKVIGCGMGAKIAGFDWFPSLRIGVGMIPRMGVELAMLSVAMASGVVGGDALTVAILVVFTTTIITPPLLKWMYSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P659</accession>
    <name>A0A218P659_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_02390</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853520.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P659"/>
    <dbReference type="GeneID" id="33315083"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02390"/>
    <dbReference type="OrthoDB" id="27736at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051400">
        <property type="entry name" value="HAD-like_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46470:SF2">
        <property type="entry name" value="GLYCERALDEHYDE 3-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46470">
        <property type="entry name" value="N-ACYLNEURAMINATE-9-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="231" mass="27072" checksum="0A4070FA22718668" modified="2017-09-27" version="1">MLVLVDLDDTLCNTWEAGKYSILRLIPHLIRKRKFKAFFYIITARYRELEQSRELHTLDFDKLVERFLTKVYSKIRPEELDEITDLVDRVFFSNLRLYPDAVPFLRGLKSMGAKVVLVTDSSTKWQRKKLEYLGIKDYFDALIISGETGHSKLEPHNFRLAKHIFPDDEVYVVGDRDDTDMRGGKEIGATTILVQRGYFRGRTAKHADYVVKDLTEALEVIKREHEKRNQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P660</accession>
    <name>A0A218P660_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Potassium transporter TrkA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_02505</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P660"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02505"/>
    <dbReference type="OrthoDB" id="27588at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="entry name" value="Regulator of K+ conductance, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="TrkA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="2">
        <location>
            <begin position="142"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="257" mass="28781" checksum="39FDA20C70ED4AD0" modified="2017-09-27" version="1">MLALVSLILVITLSMIIVRIGAIALEMTGLSRDIAAFQAQSAFSGTGFTTSESEYVVSHPVRRKIIRVLIFLGSAGITSAIATLVMAFAGKSRNETITSISILGLSLILLYLIFTSKRIERWMRRWIKRFLARAFPELRVYDYSQLLGITKGYSISQIRVKRNSWLANKTLRELELDKEGILVLGIYRKTEKGEVYLGAPKGDTVILPGDLLICYGPEEALINLSERLKGSKGEREHEEAIKKAELRRTEEEIMANT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P670</accession>
    <name>A0A218P670_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">A-type ATP synthase subunit I</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">A3L08_02575</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853554.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P670"/>
    <dbReference type="GeneID" id="33315121"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02575"/>
    <dbReference type="OrthoDB" id="85892at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016471">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22249">
        <property type="entry name" value="UDM1_RNF168_RNF169-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004428">
        <property type="entry name" value="PRK05771.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF63">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="376"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="420"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="465"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="499"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="530"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="572"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="600"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="236"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="668" mass="75408" checksum="F207624F7330243E" modified="2017-09-27" version="1">MFRPEEMVKIEVITLNRYKDSLLTYLHENGAVEIRELDVKTAQKDSPNEYHRKAASYSITISRLVDFLKAYRRSTGGGIKEFIFPQERVKKKYLYEGIEKLIKDVESFLAQVEPEIKAVEGKITSTQTEIERIKGEIAVLELLTSLNIDVSYLRSTDMLEIVVGTVDRDRFRPLMEEILKATGRRAVVVSKDFKDKILAVFVFLKSDYEKANPILAKYSLERIEIPEGKGTPKELIREYEEKLRAKEEELESAKKDAEMLAEKYYDDVVFYQELMENERDKSTALPMLARTNMTFAMTGWLPRSEVPKVLEGIKKITEGKAYINIREPSEDELDDVPIKLKNPGWARPFEMLTQMYGVPKYNEIDPTPIIAFTYSFFFGFMLTDFLYGLIVGIVAALLVKGHKKFNDGTYKFSYTLLWSSFFTMAMGALFGSFFGNALDIAGAALTGNPDFQMWRIADPLRDPMFVLMLALAVGLAHLFLGYTIGFVVKWKNGDKKGALFDQLPWMLVILAIALFATGKDVPAFVNSAKALFGIGLVIFAIGEILNNGGLAALMIISDFFGFVGNWLSYARLMALALATSGIAIVINVLVQMVWGIKFLYIGPIIGVILFFGGHLFSTAINALGAFVHSLRLHYVEFFGTFYSGEGKPFEPFKSRREVSEVTLKVENA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P684</accession>
    <name>A0A218P684_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein-export membrane protein SecF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">secF</name>
        <name evidence="11" type="ORF">A3L08_02555</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in protein export.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Part of the protein translocation apparatus. Forms a complex with SecD.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SecD/SecF family. SecF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853550.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P684"/>
    <dbReference type="GeneID" id="33315117"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02555"/>
    <dbReference type="OrthoDB" id="85411at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01464_A">
        <property type="entry name" value="SecF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022813">
        <property type="entry name" value="SecD/SecF_arch_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048634">
        <property type="entry name" value="SecD_SecF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024921">
        <property type="entry name" value="SecF_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006356">
        <property type="entry name" value="PRK08578.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081:SF8">
        <property type="entry name" value="PROTEIN TRANSLOCASE SUBUNIT SECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081">
        <property type="entry name" value="PROTEIN-EXPORT MEMBRANE PROTEIN SEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02355">
        <property type="entry name" value="SecD_SecF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="186"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="234"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="271"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Protein export membrane protein SecD/SecF C-terminal" evidence="10">
        <location>
            <begin position="121"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="304" mass="33132" checksum="4F22C7968326DF76" modified="2017-09-27" version="1">MSKPKIKEKPTASDAVRARKRKSLSFLAQMEYRKMILYPLVVFLVALIILAVHFPRLGIDLRGGVVVTAYGVSANPDQLAKDLSGKLGVEVRVESFTGVDNKGVRIYAPVGTDPTGIIRIMKEMYPDAEYTHSEVQPTFGEISQKQGLRAVALAFVAMAVVVFLFFRDPVPSMTIIFSALSDMTIALALMGLFGIELTTATIAALLMLIGYTVDSNILLTTKLLKRKEDTVDKAYLSAVSTGFTMSTTTLGALLVLWIISTSQTIDNIAIVLIFGLLADFMNTWVLNAGVLKWYISKNPRGGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6A8</accession>
    <name>A0A218P6A8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dihydroorotase</fullName>
            <shortName evidence="4">DHOase</shortName>
            <ecNumber evidence="4">3.5.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pyrC</name>
        <name evidence="6" type="ORF">A3L08_02650</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reversible cyclization of carbamoyl aspartate to dihydroorotate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24296"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="EC" id="3.5.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class I DHOase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.3" evidence="4"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853569.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6A8"/>
    <dbReference type="GeneID" id="33315136"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02650"/>
    <dbReference type="OrthoDB" id="50279at2157"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00117"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004038">
        <property type="term" value="F:allantoinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004151">
        <property type="term" value="F:dihydroorotase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006145">
        <property type="term" value="P:purine nucleobase catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01318">
        <property type="entry name" value="DHOase_IIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00220_A">
        <property type="entry name" value="PyrC_classI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004722">
        <property type="entry name" value="DHOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050138">
        <property type="entry name" value="DHOase/Allantoinase_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003271">
        <property type="entry name" value="PRK04250.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00857">
        <property type="entry name" value="pyrC_multi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43668">
        <property type="entry name" value="ALLANTOINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43668:SF2">
        <property type="entry name" value="ALLANTOINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00483">
        <property type="entry name" value="DIHYDROOROTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 4" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="5">
        <location>
            <begin position="49"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="60"/>
            <end position="62"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="291"/>
            <end position="292"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="4">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="409" mass="45683" checksum="334E7975FAB1BBE0" modified="2017-09-27" version="1">MYDLVLKGKFLMGKKLMEGSIGVSEGKISRISIGELEGEEVIQIGRGKVILPGLIDTHVHLRDFDQRDKETVESGTKAALHGGITTVFDMPNTKPPVMDATTFKRRKGLFERKAYSDYALGFLLKGNCGEAQKVRADFYKVFMGASTGSIFSENFESDYSCSPGIVSVHAEDPETIRKSPERPPEAEVRAIERAIHAAGELQKQLNICHVSTGDGLRAILEAGLPWVSFEVTPHHLFLTKRDYERNPLLKVYPPLRGESDRKALWENFSRIPIIASDHAPHTLQDKENGAAGIPGLETEVALLLDAVNRGLIELQDIVEKMHLNPIRIFGIRDKGLEVGKDADFTVIDLKKEWVVRPEDFYTKAGWSPWEGKRLRGKVVKTFLRGELVMEDDEVIGKPSGRRVEIAEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6B7</accession>
    <name>A0A218P6B7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glycerol-3-phosphate ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_02800</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853598.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6B7"/>
    <dbReference type="GeneID" id="33315166"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02800"/>
    <dbReference type="OrthoDB" id="18368at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055052">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03301">
        <property type="entry name" value="ABC_MalK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000042">
        <property type="entry name" value="Maltose/maltodextrin ABC transporter, ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015855">
        <property type="entry name" value="ABC_transpr_MalK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047641">
        <property type="entry name" value="ABC_transpr_MalK/UgpC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040582">
        <property type="entry name" value="OB_MalK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008653">
        <property type="entry name" value="PRK11650.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43875">
        <property type="entry name" value="MALTODEXTRIN IMPORT ATP-BINDING PROTEIN MSMX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43875:SF1">
        <property type="entry name" value="OSMOPROTECTIVE COMPOUNDS UPTAKE ATP-BINDING PROTEIN GGTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17912">
        <property type="entry name" value="OB_MalK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="MOP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="4"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="372" mass="41861" checksum="B01C372420832E0D" modified="2017-09-27" version="1">MAGVKLINVWKQFGDFTAVREMNLDIKDGEFMILLGPSGCGKTTTLRMIAGLEEPTRGQIYIGDNLVADPEKGVFVPPKDRDIAMVFQSYALYPHMTVYDNIAFPLKLRKVPKQEIDQRVREVAEMLGLTEFLKRKPRELSGGQRQRVALGRAIVRKPQVFLMDEPLSNLDAKLRVKMRAELKKLQRQLGVTTIYVTHDQVEAMTMGDRIAVINRGELQQVGTPEEVYDKPANTFVAGFIGSPPMNFMDATITEDGFVDFGPFRLKLLPDQVEVLEEKGYLGKDVIFGIRPEDLYDAMFAQVKVPGENMVRAMVDIIENLGSEKIVHLRVGNVTFLGSFRSESKVVEGQEVDVVFDMRKVHIFEKGSGKAVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6B9</accession>
    <name>A0A218P6B9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SAM-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_02760</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853590.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6B9"/>
    <dbReference type="GeneID" id="33315158"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02760"/>
    <dbReference type="OrthoDB" id="30774at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052907">
        <property type="term" value="F:23S rRNA (adenine(1618)-N(6))-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010286">
        <property type="entry name" value="METTL16/RlmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13393:SF0">
        <property type="entry name" value="RNA N6-ADENOSINE-METHYLTRANSFERASE METTL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13393">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="3">
        <location>
            <begin position="85"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="247" mass="27879" checksum="A9FBA039B8604F40" modified="2017-09-27" version="1">MPLWKDGKLGLPVKEAVKLFPELEKYLDERGRLDFSNRKARILYNRAIAKALFGLDVEYHPKGLVTTPVSRYLFMKTFLRGGERVLEIGAGHTALMTLLAAKIFNCDVTATELDDEFFEYARRNIEKNRANVRLIKSDGGIIRGVIPEGKTFDVIFSAPPYYEAPTKGVLTEREGVGGGRYGEEFSVRLIEEALDYLRPGGKVALFLPDKKPLINAITKRGEELGYSVRDVRFKAGTRWRHSLILAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6C9</accession>
    <name>A0A218P6C9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">V-type ATP synthase subunit H</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853553.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6C9"/>
    <dbReference type="GeneID" id="33315120"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02570"/>
    <dbReference type="OrthoDB" id="101876at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028987">
        <property type="entry name" value="ATP_synth_B-like_membr_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014275">
        <property type="entry name" value="ATPase_A1A0-cplx_hsu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02926">
        <property type="entry name" value="AhaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006256">
        <property type="entry name" value="PRK08404.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81573">
        <property type="entry name" value="F1F0 ATP synthase subunit B, membrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="103" mass="11681" checksum="3EC2B8868DC039DB" modified="2017-09-27" version="1">MEDVIKQIVDAEKEAEERIEKAKEEAKEIVLKAREEAKLIEKEVIEDAEKQARILIEKARLEGEDEAKKILEEGEREIDELKVKATNNLERAISAGIALVRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6F2</accession>
    <name>A0A218P6F2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Rubrerythrin family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_02950</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853625.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6F2"/>
    <dbReference type="GeneID" id="33315196"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02950"/>
    <dbReference type="OrthoDB" id="100896at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="144" mass="16665" checksum="18EAD456E2475877" modified="2017-09-27" version="1">MNLNELLERLVHQENELYNLHKLGETFATFERPELAEVFQLIAEEELRHRKTLEDMLSGKTLEGTAVIDYLDSLSLEPMLDDARAEPESLEELVIEALIREKHAYELYMKLATILDGSLAQIFKMMGGEELKHAYRLKLVYEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6I4</accession>
    <name>A0A218P6I4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 20">Inosine-5'-monophosphate dehydrogenase</fullName>
            <shortName evidence="13">IMP dehydrogenase</shortName>
            <shortName evidence="13">IMPD</shortName>
            <shortName evidence="13">IMPDH</shortName>
            <ecNumber evidence="13 20">1.1.1.205</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">guaB</name>
        <name evidence="22" type="ORF">A3L08_03190</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13 20">
            <text>IMP + NAD(+) + H2O = XMP + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="13">Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13 20">Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 13">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13 19">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.205" evidence="13 20"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853663.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6I4"/>
    <dbReference type="GeneID" id="33315244"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03190"/>
    <dbReference type="OrthoDB" id="21361at2157"/>
    <dbReference type="UniPathway" id="UPA00601">
        <property type="reaction ID" value="UER00295"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006177">
        <property type="term" value="P:GMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04601">
        <property type="entry name" value="CBS_pair_IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00381">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000003">
        <property type="entry name" value="GMP reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01964">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005990">
        <property type="entry name" value="IMP_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015875">
        <property type="entry name" value="IMP_DH/GMP_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01302">
        <property type="entry name" value="IMP_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911:SF111">
        <property type="entry name" value="INOSINE-5'-MONOPHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911">
        <property type="entry name" value="INOSINE-5-MONOPHOSPHATE DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00478">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000130">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01240">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51412">
        <property type="entry name" value="Inosine monophosphate dehydrogenase (IMPDH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00487">
        <property type="entry name" value="IMP_DH_GMP_RED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 18" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6 13 20" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="4 13 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13 16" id="KW-0520">NAD</keyword>
    <keyword evidence="9 13 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 13 17" id="KW-0630">Potassium</keyword>
    <keyword evidence="7 13 20" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="21">
        <location>
            <begin position="99"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="21">
        <location>
            <begin position="156"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="active site" description="Thioimidate intermediate" evidence="13 14">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13 14">
        <location>
            <position position="397"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="247"/>
            <end position="249"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 16">
        <location>
            <begin position="294"/>
            <end position="296"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13 17">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13 17">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 15">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13 17">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 15">
        <location>
            <begin position="334"/>
            <end position="336"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 15">
        <location>
            <begin position="357"/>
            <end position="358"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 15">
        <location>
            <begin position="381"/>
            <end position="385"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 15">
        <location>
            <position position="412"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="466"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="467"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="468"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU003928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="ASJ06405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="485" mass="52253" checksum="5134E9182BB005B0" modified="2017-09-27" version="1">MGRFEHKLVNAIKGYTFDDVLLIPQATEVEPKDVDVSTQITPNVRLKVPILSAAMDTVTEWEMAVAMAREGGLGVIHRNMSIEEQVEQVKKVKRAERFIVEDVISISPEETVDYALFLMEKNDIDGLPVVEDGRVVGVISKKDIAVKQGKLVKEVMTSEPITVLESVTAEEALNLMFEHRIDRLPVVDENGKLVGIITMSDLAKRRKYKNAVRDENGDLLVAAAVGPFDLERAKALDKAGADVIVVDTAHAHNLKAIKAMKEIRKAVDADLIVGNIANPKAVDDLTFADAVKVGIGPGSICTTRVVAGVGVPQLTAVALVADRAQEYGLYVIADGGIRYSGDIVKAIAAGADAVMLGSLLAGTKEAPGKEVVINGRRYKQYRGMGSLGAMMKGGAERYYQKGHMKTRKFVPEGVEGVVPYKGSVSDVLYQLVGGLRSGMGYVGAANIGELKERGEFVIITNAGVKESHPHDILITNEAPNYPVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6J0</accession>
    <name>A0A218P6J0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphodiesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="OrthoDB" id="33550at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF120">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="500" mass="58088" checksum="0E628B3298FDE671" modified="2017-09-27" version="1">MNEKLVIIGIDSLDPYVILEHRDELPTFSQLIKESSTFLSKSVFPVDTIPAWVSIYTGFHPGNHGVLYVYDVFDPNLSDLRKLDISHIKGKTFWDYASEAGYKVAVLYPTLMFPTWKVNGVMISKSPYDRRVNDIQTSRDISCYPEKIKEKYGIPTEVKDIWGGYPGKDKLAEWASLGKDAVLEDFNMAFRIYKGEKWDMFFVYFSHLDIIQHRLWRFFDRKDPMYRYDQKLTPIILDFYKLFDNIIGKFINELPDAQFIVLSDHGHQSRPIKTLNINEYLRQNKLLKAKKVNILGIFRKIMLNIITTLNLEKHAIRIISRSHYLTKYSKQAYSSSGLILKEESKAWLSTFAGIKSYPHGGIEINSEIVSKKEYDELVSEIIKLLYTLRGPNGEQLVKWARRREDVYNGKYTEQIFPDILFELKDDWGVGWELGRLYGKSHDHKVASGGHRKDAVLLLRNIRRDVVRKNDISIVDMAPSILDLMNVDTSSLKLDGKSIFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6R1</accession>
    <name>A0A218P6R1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ribonucleoside-triphosphate reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03610</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853736.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6R1"/>
    <dbReference type="GeneID" id="33315327"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03610"/>
    <dbReference type="OrthoDB" id="84929at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="126" mass="14470" checksum="CC7D10E3DBD60BC5" modified="2017-09-27" version="1">MEFKEEIGKALEGDGLWTVVTFKTPHGPGITLEKLAEAAESAGWRITFRANWWTADIPYGLARLDLRKNGREKILLGKWILGSKCELISLENMSLERGRDEFFRMVDSITSTLIHDPVIRTMREQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6S7</accession>
    <name>A0A218P6S7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Digeranylgeranylglyceryl phosphate synthase</fullName>
            <shortName evidence="12">DGGGP synthase</shortName>
            <shortName evidence="12">DGGGPS</shortName>
            <ecNumber evidence="12">2.5.1.42</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">(S)-2,3-di-O-geranylgeranylglyceryl phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Geranylgeranylglycerol-phosphate geranylgeranyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ubiA</name>
        <name evidence="13" type="ORF">A3L08_03605</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C2 hydroxyl of (S)-3-O-geranylgeranylglyceryl phosphate (GGGP). This reaction is the second ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>sn-3-O-(geranylgeranyl)glycerol 1-phosphate + (2E,6E,10E)-geranylgeranyl diphosphate = 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18109"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57677"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="ChEBI" id="CHEBI:58837"/>
            <dbReference type="EC" id="2.5.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="12">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the UbiA prenyltransferase family. DGGGP synthase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.42" evidence="12"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853735.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6S7"/>
    <dbReference type="GeneID" id="33315326"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03605"/>
    <dbReference type="OrthoDB" id="11851at2157"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047295">
        <property type="term" value="F:geranylgeranylglycerol-phosphate geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13961">
        <property type="entry name" value="PT_UbiA_DGGGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1780">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01286">
        <property type="entry name" value="DGGGP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023547">
        <property type="entry name" value="DGGGP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050475">
        <property type="entry name" value="Prenyltransferase_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009522">
        <property type="entry name" value="PRK12883.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009523">
        <property type="entry name" value="PRK12884.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42723">
        <property type="entry name" value="CHLOROPHYLL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42723:SF1">
        <property type="entry name" value="CHLOROPHYLL SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 12" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="89"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="142"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="258"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="276" mass="29661" checksum="AA9832C5DF653A1E" modified="2017-09-27" version="1">MEFKAFIEITRPHNCLLAGIVGVLGSIVAVGDIPAPRTALLVFLVVVLGCAGGNTINDYFDYEIDKINRPERPLPRGAMSRRTALYYSLALFAVGLSLAALINIYAFTIALVAYVMMFLYAWKLKPLPFVGNLTVAALTAATPLYGAVAVEHLGLAGYLAVCAFLVNVAREVIKDIEDVEGDVAKGARTLPILWGNKKAARVGALFAVLTVAASFLPVKAGVGLGYYAMVPVDLIILYAAYLILRSQDRETAHRSQKLLKISIFLAVMAFLVAALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6T6</accession>
    <name>A0A218P6T6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">DNA polymerase</fullName>
            <ecNumber evidence="2 10">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">A3L08_03720</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 15">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="2 10"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853754.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6T6"/>
    <dbReference type="GeneID" id="33315349"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03720"/>
    <dbReference type="OrthoDB" id="323192at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05780">
        <property type="entry name" value="DNA_polB_Kod1_like_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05536">
        <property type="entry name" value="POLBc_B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.342.10:FF:000015">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000013">
        <property type="entry name" value="DNA polymerase Pol2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050240">
        <property type="entry name" value="DNA_pol_type-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00592">
        <property type="entry name" value="pol2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322">
        <property type="entry name" value="DNA POLYMERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322:SF23">
        <property type="entry name" value="DNA POLYMERASE DELTA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="5 10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="12">
        <location>
            <begin position="130"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="11">
        <location>
            <begin position="351"/>
            <end position="746"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="774" mass="89995" checksum="A5906575AEE5B66D" modified="2017-09-27" version="1">MILDADYITEDGKPVIRIFRKEKGEFKIEYDRNFEPYFYALLKDDSAIEDVKKITAERHGTTVRVVRAEKVKKKFLGRPIEVWKLYFTHPQDVPAIRDKIREHPAVVDIYEYDIPFAKRYLIDKGLIPMEGDEELKMLAFDIETLYHEGEEFAEGPILMISYADEEGARVITWKNIDLPYVDVVSTEKEMIKRFLRVIKEKDPDVLITYNGDNFDFAYLKKRSEKLGVKFILGRDGSEPKIQRMGDRFAVEVKGRIHFDLYPVIRRTINLPTYTLEAVYEAIFGQPKEKVYAEEITQAWETGEGLERVARYSMEDAKVTYELGKEFFPMEAQLSRLVGQSLWDVSRSSTGNLVEWFLLRKAYERNELAPNKPDEKELARRRESYAGGYVKEPEKGLWENIVYLDFRSLYPSIIITHNVSPDTLNREGCKEYDVAPQVGHRFCKDFPGFIPSLLGDLLEERQKIKKNMKATIDPIEKKLLDYRQRAIKILANSFYGYYGYAKARWYCRECAESVTAWGRQYIETTIREIEDKFGFKVLYADTDGFFATIPGADAETVKKKSKEFLKYINAKLPGLLELEYEGFYKRGFFVTKKKYAVIDEEDKITTRGLEIVRRDWSEIAKETQARVLEAILKHGDVEEAVKIVKEVTEKLSKYEVPPEKLVIHEQITRDLKDYKATGPHVAVAKRLAARGIKIRPGTVISYIVLKGSGRIGDRAIPFDEFDPTKHKYDAEYYIENQVLPAVERILRAFGYRKEDLRYQKTRQVGLGAWLGMGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6W6</accession>
    <name>A0A218P6W6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HIT family hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_03835</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853777.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6W6"/>
    <dbReference type="GeneID" id="33315372"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03835"/>
    <dbReference type="OrthoDB" id="26806at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052908">
        <property type="entry name" value="AP-4-A_phosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42997:SF1">
        <property type="entry name" value="AP-4-A PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42997">
        <property type="entry name" value="HIT FAMILY HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="2">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="1">
        <location>
            <begin position="92"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="150" mass="17316" checksum="030A3A89E69E881D" modified="2017-09-27" version="1">MECPFCNPDKEALLYEDGLIRILIDSYPANRGHLLVVPKRHVESWEELREEEKLALLRGMELAMEKLRGTLKPDGFNVGMNLGRAAGQTVPHLHLHVIPRWEGDCRSPRGGVRKAVLDLEDENLNLRERWVRNRLSGEEIERLRAAFSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Y0</accession>
    <name>A0A218P6Y0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADH dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_03990</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 24 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853804.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Y0"/>
    <dbReference type="GeneID" id="33315402"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03990"/>
    <dbReference type="OrthoDB" id="70332at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03064">
        <property type="entry name" value="TRX_Fd_NuoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1590:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000015">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1590">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028431">
        <property type="entry name" value="NADP_DH_HndA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002023">
        <property type="entry name" value="NuoE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042128">
        <property type="entry name" value="NuoE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041921">
        <property type="entry name" value="NuoE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01958">
        <property type="entry name" value="nuoE_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005722">
        <property type="entry name" value="PRK07539.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43342:SF1">
        <property type="entry name" value="BIFURCATING [FEFE] HYDROGENASE GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43342">
        <property type="entry name" value="NADH-QUINONE OXIDOREDUCTASE, E SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01257">
        <property type="entry name" value="2Fe-2S_thioredx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000216">
        <property type="entry name" value="NADH_DH_24kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01099">
        <property type="entry name" value="COMPLEX1_24K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="154" mass="17245" checksum="6D394D76B54A66AD" modified="2017-09-27" version="1">MEADLDYIRSYPPEPSSLIPLLQRTQERFGYLPREALEEIASYLGIPLSRVYGVATFYAQFRFEPLGKYVVKICHGTACHVNGAVNISQAIREELGIEEGQTTEDGLVTLERVACLGCCSLAPVLMVNEKVFGKLTPEKVRKLIRKLREGKLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Y9</accession>
    <name>A0A218P6Y9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03980</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_157721584.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Y9"/>
    <dbReference type="GeneID" id="33315400"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03980"/>
    <dbReference type="OrthoDB" id="86053at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11570">
        <property type="entry name" value="Protein of unknown function DUF257"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005489">
        <property type="entry name" value="DUF257"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03192">
        <property type="entry name" value="DUF257"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="222" mass="24972" checksum="37F98E777C36B96D" modified="2017-09-27" version="1">MDTSVLKIIESVKPGEIALLEYLPSYIPELTLLKLMEYSEEMKIPLIIDDNFDTLPVIAAHIENLGIKVDFSGVYVIKTGGRIDVGNVVARVPFHPDPRVYIKNYEKEASKVLEDVEDSINLVLGLENFLKTLSSPSDIYLVIWILQRFLGNTRRRAFYLLNKKILDSLSPIISSEMKRVASTVVEMVPYPTGAILRFVKSTNVDLFGEELKVDIVDIGGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P711</accession>
    <name>A0A218P711_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rpl14</name>
        <name evidence="9" type="ORF">A3L08_04100</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L24e, part of which may contact the 16S rRNA in 2 intersubunit bridges.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853825.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P711"/>
    <dbReference type="GeneID" id="33315424"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04100"/>
    <dbReference type="OrthoDB" id="23569at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00337">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.150.20:FF:000007">
        <property type="entry name" value="50S ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_uL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019971">
        <property type="entry name" value="Ribosomal_uL14_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_uL14_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006344">
        <property type="entry name" value="PRK08571.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03673">
        <property type="entry name" value="uL14_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L14/L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761:SF8">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00066027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="141" mass="15137" checksum="8D10D5367816D306" modified="2017-09-27" version="1">MAKKGAGATRGISPVRPTRALPVGAYLNVADNSGAKVIQIIGVVGYKGTRRRLASAGVGDMVIATVKKGRPDIRHQVVRAVVVRQRKEYRRLDGMRVKFEDNAAAIVTPEGVPRGTEIRGAIAREAAERWVRLGSIASIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P735</accession>
    <name>A0A218P735_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rps11</name>
        <name evidence="10" type="ORF">A3L08_04225</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Located on the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853847.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P735"/>
    <dbReference type="GeneID" id="33315449"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04225"/>
    <dbReference type="OrthoDB" id="12054at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.80:FF:000007">
        <property type="entry name" value="30S ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="entry name" value="Ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_uS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_uS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019961">
        <property type="entry name" value="Ribosomal_uS11_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_uS11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_uS11_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03628">
        <property type="entry name" value="arch_S11P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007176">
        <property type="entry name" value="PRK09607.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="140" mass="15112" checksum="7B1FBE48BB755574" modified="2017-09-27" version="1">MSEEQTQQVNIKKKEKWGVAHIYSSYNNTIIHITDLTGAETVSRWSGGMVVKADRDEPSPYAAMIAARRAAEEAMEKGFVGVHIKVRAPGGSKSKSPGPGAQAAIRALARAGLRIGRVEDVTPIPHDGTRPKGGRRGRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P751</accession>
    <name>A0A218P751_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_04340</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853864.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P751"/>
    <dbReference type="GeneID" id="33315472"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04340"/>
    <dbReference type="OrthoDB" id="84757at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="69" mass="7906" checksum="B4D9CD478BB6AAAF" modified="2017-09-27" version="1">MTTVIRKDAERFLKELRTHYGDVWKMPRSNYLSKPDFIVIDPKSGKKTKVSFVSLDDGEVVGVVYDELG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P760</accession>
    <name>A0A218P760_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Threonine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_04375</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853869.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P760"/>
    <dbReference type="GeneID" id="33315479"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04375"/>
    <dbReference type="OrthoDB" id="85597at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01563">
        <property type="entry name" value="Thr-synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006205">
        <property type="entry name" value="PRK08329.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF6">
        <property type="entry name" value="L-THREONINE DEHYDRATASE CATABOLIC TDCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="4">
        <location>
            <begin position="78"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="354" mass="39031" checksum="84D28D9C76F2E0F9" modified="2017-09-27" version="1">MLRCTRCGKAYPETFRLRCDCGETLLVEREGYDFFGSLLPYLDIRRYLNFLPIGGNYLPPAIPAITPTTSVQAGPVFALFKLEYLQPSGSFKDRGTFVTVAKLMEEDINEVVLDSSGNAALSLALYSLPAGIKAHLFVSYDTMPEKLSLLQSLGAVIHFVEGDRMAVHEKAEEFAEREGLTYVSHWLNPYFLEGTKTAAFEVYEQVGVPDYVLVPTGSGTLFLGLWKGFKELEKMGEIARLPVFVAVQAAGYESLCERSKLPNPLADGIAIPQPPRLKEMERVLDETGGLCVSVDEHETREALSWLKRAGFLVEPTSAVVLAAMWKLIERGEISDGSRVLLPLTGSGLKLIEGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P761</accession>
    <name>A0A218P761_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nitrosopumilus output domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_04405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853873.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P761"/>
    <dbReference type="GeneID" id="33315485"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04405"/>
    <dbReference type="OrthoDB" id="85374at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.200">
        <property type="entry name" value="Protein of unknown function DUF3227"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021609">
        <property type="entry name" value="NitrOD5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044908">
        <property type="entry name" value="NitrOD5-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11537">
        <property type="entry name" value="NitrOD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitrosopumilus output" evidence="1">
        <location>
            <begin position="3"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="99" mass="10699" checksum="D8C0035EEEDC9976" modified="2017-09-27" version="1">MSKGAEILTKALSAALEKVNPGLKAVLETHLRVTTGKGLELAYEDPSAFKAAVGRLFGEYSARLLEMVVIDSLKDRFGGSVTVESLEDLVSELKRIYGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P780</accession>
    <name>A0A218P780_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Na+dependent transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_04520</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P780"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04520"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42948">
        <property type="entry name" value="TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42948:SF1">
        <property type="entry name" value="TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="326"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="367"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="392"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="433"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="466"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="502" mass="55814" checksum="C4437590BADB39B4" modified="2017-09-27" version="1">MAGNAIGLGNFVRFPTQVAQNGGGAFMVPYFVALFFLGIPVMWIEWVAGRYGGKYGHGTLGPTYYLMARESLKPRSALWFGVIAGMLAFSLTVLLNSYYLHLIGWSAAYSWFSITGAYFGQNTGEFFSNYLSNYGQVFLFWGITVILLAIAVGQGVSKGIERWVKVIMPLLYVFAILMVGYIFVLGSPVDPNWSTIDGFKFIWSPNWSYLGDHLWEVMLAATGQIFFTLSLGMGIIQNYASYLGPKDDVALSGIATVSLNEFAEVVLGGSIAIPLATAYAPKIVPPEILAKGKSEALSWIGHEFGLGFSYTSLPNVFVSMGDAGRIFGALWFLLLWFAGFTSAIAMYNYLTALLEEDLNIKRSTGTWLVLLLYLVMGLPVVYISGYMDQVDAWVNFQLTLLALFDIIIAVYLFKPDNFWKELHEGAWMTVPSWYKPIILYIAPILLLVPMIGMFKSLVGSTLEWPARIAIIVMLVIGAVESYYSIKKKYAKELEKNEVIIRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P792</accession>
    <name>A0A218P792_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glyceraldehyde 3-phosphate phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_04615</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the dephosphorylation of D,L-glyceraldehyde 3-phosphate in vitro.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853910.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P792"/>
    <dbReference type="GeneID" id="33315527"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04615"/>
    <dbReference type="OrthoDB" id="31229at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051400">
        <property type="entry name" value="HAD-like_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011950">
        <property type="entry name" value="HAD-SF_hydro_IA_CTE7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02253">
        <property type="entry name" value="CTE7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46470:SF2">
        <property type="entry name" value="GLYCERALDEHYDE 3-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46470">
        <property type="entry name" value="N-ACYLNEURAMINATE-9-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01135">
        <property type="entry name" value="C1.5.6:_HAD__Beta-PGM__Phospha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01228">
        <property type="entry name" value="COF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="214" mass="24827" checksum="68DF93AA4860DF96" modified="2017-09-27" version="1">MKAVFFDIDGTLLTEMPLIELFLPQVYEKLSKKLGIDRESARERFLREIFGRRDTYDWHDWNFFFKLFDLDMDYSELMKRYPHKIHVYPDTLPTLEWLKGEGYKLGVITSGPAYQRLKLELTGLSGYFDVIVTRDDVKAIKPEPKIFLHALENAGVEPEEALMVGDSLSQDVYGAKNVGMTAVWINRDGEPGYHMADYEIRTLHELRKILGGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7C3</accession>
    <name>A0A218P7C3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9 10">Cell division protein FtsZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ftsZ</name>
        <name evidence="13" type="ORF">A3L08_04800</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. Polymerizes to form a dynamic ring structure in a strictly GTP-dependent manner. Interacts directly with several other division proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="8">Assembles at midcell at the inner surface of the cytoplasmic membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 10">Belongs to the FtsZ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853940.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7C3"/>
    <dbReference type="GeneID" id="33315564"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04800"/>
    <dbReference type="OrthoDB" id="371908at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02201">
        <property type="entry name" value="FtsZ_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000008">
        <property type="entry name" value="Cell division protein FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000023">
        <property type="entry name" value="Cell division protein FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00909">
        <property type="entry name" value="FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000158">
        <property type="entry name" value="Cell_div_FtsZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020805">
        <property type="entry name" value="Cell_div_FtsZ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045061">
        <property type="entry name" value="FtsZ/CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024757">
        <property type="entry name" value="FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00065">
        <property type="entry name" value="ftsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314">
        <property type="entry name" value="CELL DIVISION PROTEIN FTSZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314:SF3">
        <property type="entry name" value="MITOCHONDRIAL DIVISION PROTEIN FSZA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12327">
        <property type="entry name" value="FtsZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00423">
        <property type="entry name" value="CELLDVISFTSZ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01134">
        <property type="entry name" value="FTSZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01135">
        <property type="entry name" value="FTSZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 8 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 10" id="KW-0717">Septation</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="11">
        <location>
            <begin position="43"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="12">
        <location>
            <begin position="236"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="51"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="138"/>
            <end position="140"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="NCBIfam" id="TIGR00065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="374" mass="40229" checksum="3180134256E2546D" modified="2017-09-27" version="1">MLKLIEDAIERTSAAPNKIPEAQAPQTDIDEELKRILEQIQAKIYVVGVGGAGCNTINRMMQVGIQGAKVIAINTDAQDLLKVRAHKKILIGKELTRGLGAGNNPKMGEEAAKESEREIREALEGADMVFITCGLGGGTGTGAAPVVAEMAKKMGALTVSVVTLPFTVEGIRRIKNAEYGLERLRKNSDTVIVIPNDKLMEVAPNLPIHMAFKVADEILVQAVKGITELITKPGLVNLDFNDVRAVMKDGGVAMIGIGESDSEKRALEAAQQALNSPLLDVDISGAKGALISISGSDVKLEEAQQIIELVTSKLDPEAQVIWGIQLDEELNKMIRILIVVTGVSSPYAVAGEEETSYYGEEQERKIIKLDLEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7F7</accession>
    <name>A0A218P7F7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphoenolpyruvate carboxykinase [GTP]</fullName>
            <shortName evidence="10">PEP carboxykinase</shortName>
            <shortName evidence="10">PEPCK</shortName>
            <ecNumber evidence="10">4.1.1.32</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">GTP-dependent phosphoenolpyruvate carboxykinase</fullName>
            <shortName evidence="10">GTP-PEPCK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pckG</name>
        <name evidence="13" type="ORF">A3L08_04885</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>oxaloacetate + GTP = phosphoenolpyruvate + GDP + CO2</text>
            <dbReference type="Rhea" id="RHEA:10388"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.1.1.32"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="10">Binds 1 Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.32" evidence="10"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853956.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7F7"/>
    <dbReference type="GeneID" id="33315581"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04885"/>
    <dbReference type="OrthoDB" id="55875at2157"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004613">
        <property type="term" value="F:phosphoenolpyruvate carboxykinase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071333">
        <property type="term" value="P:cellular response to glucose stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046327">
        <property type="term" value="P:glycerol biosynthetic process from pyruvate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006107">
        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019543">
        <property type="term" value="P:propionate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033993">
        <property type="term" value="P:response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00819">
        <property type="entry name" value="PEPCK_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.449.10:FF:000010">
        <property type="entry name" value="Phosphoenolpyruvate carboxykinase [GTP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.449.10">
        <property type="entry name" value="Phosphoenolpyruvate Carboxykinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.8.10">
        <property type="entry name" value="Phosphoenolpyruvate Carboxykinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00452">
        <property type="entry name" value="PEPCK_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018091">
        <property type="entry name" value="PEP_carboxykin_GTP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013035">
        <property type="entry name" value="PEP_carboxykinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008209">
        <property type="entry name" value="PEP_carboxykinase_GTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035077">
        <property type="entry name" value="PEP_carboxykinase_GTP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035078">
        <property type="entry name" value="PEP_carboxykinase_GTP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008210">
        <property type="entry name" value="PEP_carboxykinase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003253">
        <property type="entry name" value="PRK04210.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11561">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11561:SF0">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE [GTP]-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00821">
        <property type="entry name" value="PEPCK_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17297">
        <property type="entry name" value="PEPCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001348">
        <property type="entry name" value="PEP_carboxykinase_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68923">
        <property type="entry name" value="PEP carboxykinase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53795">
        <property type="entry name" value="PEP carboxykinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00505">
        <property type="entry name" value="PEPCK_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="2 10" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="7 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 10" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoenolpyruvate carboxykinase GTP-utilising N-terminal" evidence="12">
        <location>
            <begin position="30"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoenolpyruvate carboxykinase C-terminal P-loop" evidence="11">
        <location>
            <begin position="244"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="272"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="220"/>
            <end position="222"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="271"/>
            <end position="276"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="384"/>
            <end position="386"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="418"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="624" mass="72383" checksum="9F5307EE55723819" modified="2017-09-27" version="1">MEPLERLEKLLDKEQFEKIKAIDNPELHAFLAEWIEWLEPDKVFVCTDSEEDEQYVRWKALYYCEEKMLETPNHTVHYDNYYDQARDKANTAILLSGGKKLPYINTKDRDEGLKEIRELMRGVMKGKELFICFFVLGPKNSIFTIPAVQLTDSAYVAHSEFILYRKGYEEFKRLGRSARFFRFVHSTGELDERKTSKNLDKRRIYIDLEDETVYSVNTQYGGNTIGLKKLAFRLTIKRAVEEGWLSEHMFLMRVNGPHGRKTYFTGAYPSMCGKTSTAMISWENIVGDDLSFILPVNGVARGANVEKGVFGIIQGVNPEDDPIIWQVLHSPVEIIFSNVLVKDGKPYWNDMGVEIPDEGENHSGKWWRGKKDAEGNEIPPSHKNARFTVSLEHFPNADLEALENPCGVEVGGMIFGGRDADTWPPVREAFDWEHGVITMGASLESETTAATLGKEGVRAFNPMAILDFMSVHLGDYLRNYLEFGRKLRKSPKIFAVNYFLRDEKGNWLNHKLDKAVWLKWMELRVHGDVEAIETPIGYIPKYEDLRRLFDEVLNKEYTRDAYEKQFTIRVPELLAKIDRIEEIYRKLDNVPEELFRVLEEERKRLLEAREKYGDYISPFTLEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7F8</accession>
    <name>A0A218P7F8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycerophosphodiester phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_04935</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853963.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7F8"/>
    <dbReference type="GeneID" id="33315591"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04935"/>
    <dbReference type="OrthoDB" id="19020at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08568">
        <property type="entry name" value="GDPD_TmGDE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46211:SF14">
        <property type="entry name" value="GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46211">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GP-PDE" evidence="1">
        <location>
            <begin position="9"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="250" mass="28480" checksum="9EC420BBD2062555" modified="2017-09-27" version="1">MTSWESDRVLVLGHRGYSAKYPENSLLAFRKTIEASADGIELDVWLSKDGRVVIMHDETIDRTSNMTGRQKDMTLEELKKADIGMGERIPTLEEVFEVLPDNALLNIELKDRDAAGEVARIVKENNPGRVLISSFDVDALREYRKFDGKTRMGLLIDREDVVPLIPKLKDELNLWSINIPIEAIPLLGLEKTVQAVKWARSLGLKVVLWTEKDDLFYLNDNLAKLTGLFEVVIVNDVERMSNYLRKLGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7G1</accession>
    <name>A0A218P7G1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_04995</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853974.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7G1"/>
    <dbReference type="GeneID" id="33315603"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04995"/>
    <dbReference type="OrthoDB" id="31226at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050155">
        <property type="entry name" value="HAD-like_hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43434">
        <property type="entry name" value="PHOSPHOGLYCOLATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43434:SF1">
        <property type="entry name" value="PHOSPHOGLYCOLATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="225" mass="26989" checksum="E8AEB928306DB999" modified="2017-09-27" version="1">MIIAFDFDGTLADTYSCIEEAFKRALEKRYHWLPGKALWAKLLTKIELQFERPTFGKHKKKSKPPFFLRTKFFETWFEERAKLTKPIDDSPELLRKLKEAGHTVISFSAEDFIDGMKVRRLKEMGIYDLFDDVIVFGRDLTIEEAFQLVREKYGDEIFIWVDDKPWRFIGHGDENTEYVWYYFPFTARFIEKNRERLALIPHMHIIRDLWSIFDVIERVKGERSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7I1</accession>
    <name>A0A218P7I1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Translation initiation factor 2 subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 10">aIF2-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 10">eIF-2-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">eif2a</name>
        <name evidence="12" type="ORF">A3L08_05140</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 10">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the eIF-2-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853993.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7I1"/>
    <dbReference type="GeneID" id="33315632"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05140"/>
    <dbReference type="OrthoDB" id="84794at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04452">
        <property type="entry name" value="S1_IF2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000015">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.190:FF:000006">
        <property type="entry name" value="Translation initiation factor 2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1130:FF:000002">
        <property type="entry name" value="Translation initiation factor 2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1130">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.190">
        <property type="entry name" value="Translation initiation factor 2, subunit 1, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00231">
        <property type="entry name" value="eIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044126">
        <property type="entry name" value="S1_IF2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022964">
        <property type="entry name" value="TIF2_asu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024055">
        <property type="entry name" value="TIF2_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024054">
        <property type="entry name" value="TIF2_asu_middle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011488">
        <property type="entry name" value="TIF_2_asu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003062">
        <property type="entry name" value="PRK03987.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003064">
        <property type="entry name" value="PRK03987.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003066">
        <property type="entry name" value="PRK03987.1-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07541">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110993">
        <property type="entry name" value="eIF-2-alpha, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116742">
        <property type="entry name" value="eIF2alpha middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10 12" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="12"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="275" mass="31611" checksum="BC7F57E198C28FA3" modified="2017-09-27" version="1">MPRKAKEYPEEGEFVVATVKNIHNYGAFLKLDEYPGKEGFMHISEVASSWVKNIRDHIKEGQKIVAKVIRVDPEKGHIDLSLKRVNQQQRKAKLQEYKRAQKAENLLKMAAEKIGKDFETAWREVWVPLEEEYGEVYAAFEDAAQNGIEVLKGLISDEWIEALRPIIEAYVEIPTVTIDAEFEITVPKPNGIEIIKEALIRARDRANEEKEIEVKFSYQGAPRYRIDVTAPDYHKAEEVLEDIAEEILRVIKEAGGEATLIRKEKRIKKVKKRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7L7</accession>
    <name>A0A218P7L7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_05275</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854019.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7L7"/>
    <dbReference type="GeneID" id="33315659"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05275"/>
    <dbReference type="OrthoDB" id="57422at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051598">
        <property type="entry name" value="TSUP/Inactive_protease-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43701">
        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN MJ0441-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43701:SF2">
        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN YJNA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="244" mass="26069" checksum="99093C578591A2D2" modified="2017-09-27" version="1">MNYPELALISLILSIVFSIGGVGSAIAIVPTMTWLGVPLMTAKPTGLFINTLSMLSATLKNLKHGKLDHRFGLPILLVATAMAPVGAYAGKFIPKVYVLWVFLGFLLYSGTMMIFFKPRRRPKDGNHIVEGSIIGGIAGFLGGLLGVGGGGIISPALIMLGYEPKKVASTTALVVFFSSLSGFLTYWGMGALDWMLLLWVSIPAIAGGWIGTHLMHFKMSSDDVKKVIGVIIYLIALKTLANVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7M9</accession>
    <name>A0A218P7M9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05330</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854030.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7M9"/>
    <dbReference type="GeneID" id="33315670"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05330"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="61" mass="7048" checksum="6BFC81CD72C6BDE5" modified="2017-09-27" version="1">MTSIAIRVPEGVDIEFAKRLAKLVEKRLAELTELNRLLSDSELTDEDMGEVIKEFRTQRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7N6</accession>
    <name>A0A218P7N6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Arsenic resistance protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_05235</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the arsenical resistance-3 (ACR3) (TC 2.A.59) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854012.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7N6"/>
    <dbReference type="GeneID" id="33315651"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05235"/>
    <dbReference type="OrthoDB" id="326456at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015104">
        <property type="term" value="F:antimonite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015105">
        <property type="term" value="F:arsenite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004706">
        <property type="entry name" value="Arsenical-R_Acr3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002657">
        <property type="entry name" value="BilAc:Na_symport/Acr3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43057:SF1">
        <property type="entry name" value="ARSENICAL-RESISTANCE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43057">
        <property type="entry name" value="ARSENITE EFFLUX TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01758">
        <property type="entry name" value="SBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="338" mass="36966" checksum="78D2B26013DA2A0E" modified="2017-09-27" version="1">MNWLKLSNNLKKNLLWYSLVAIAVGWLLGLAFPGFAKAHKVGLSSLTTLLVFLMIYPMMINLNLGRIPQVMKEPRPVLLSLAYNFVLTPIIGFLLVKGFIHDPNLALGFLLVMLVPGSSMSIGYTGLAGGDLEVATVALGVNFLLIPVMLPLWIKLLGSAYSVPVPLSLLLRTVFIMLILPMLLGDLTRRALIRRLGPERFLSLKPLFGAITMSTMLVLVGLIFFMKAELLLSKWTLLVELAVVNTVYMVIMLGLITWLDRALGLKYGEHMAIAFLSVGKNNGTAIAIATLAFEPLVAIPAATLPIFQIIFLILYLKAAERIKCIFETCSEGGTPATV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7P3</accession>
    <name>A0A218P7P3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MBL fold hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854059.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7P3"/>
    <dbReference type="GeneID" id="33315700"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05480"/>
    <dbReference type="OrthoDB" id="7773at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07713">
        <property type="entry name" value="DHPS-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041712">
        <property type="entry name" value="DHPS-like_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052926">
        <property type="entry name" value="Metallo-beta-lactamase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13754:SF18">
        <property type="entry name" value="7,8-DIHYDROPTERIN-6-METHYL-4-(BETA-D-RIBOFURANOSYL)-AMINOBENZENE-5'-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13754">
        <property type="entry name" value="METALLO-BETA-LACTAMASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="21"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="268" mass="29370" checksum="5ADC6823997A0604" modified="2017-09-27" version="1">MKLTILYENHSGFKKGLLGGHGFSALVELDGVNVLVDTGTDGRVLLSNMGELGVGPGDIDYVFITHGHYDHTGGLKDFLKARGRPVKVIAHPEIFRRRVALKPHRREIGIPFTKEELEELGAEFVLNGKPFEFAPGLWSSGEIARRTWDRAVGYVEEEERLVKDAVPDDMTLIVDLGEKVAVVTGCGHSGVLNIAWHAEDVAGKPVKALIGGFHLIGAKRDILDEVAEEIDAEKLYAGHCTGVDSYAYLKAKLGERVEHLHVGKTIEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7T3</accession>
    <name>A0A218P7T3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10 11">L-threonine 3-dehydrogenase</fullName>
            <shortName evidence="10">TDH</shortName>
            <ecNumber evidence="9 10 11">1.1.1.103</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 13" type="primary">tdh</name>
        <name evidence="13" type="ORF">A3L08_05680</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the NAD(+)-dependent oxidation of L-threonine to 2-amino-3-ketobutyrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 10">
            <text>L-threonine + NAD(+) = (2S)-2-amino-3-oxobutanoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13161"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78948"/>
            <dbReference type="EC" id="1.1.1.103"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8 10">Amino-acid degradation; L-threonine degradation via oxydo-reductase pathway; glycine from L-threonine: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.103" evidence="9 10 11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854096.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7T3"/>
    <dbReference type="GeneID" id="33315740"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05680"/>
    <dbReference type="OrthoDB" id="73567at2157"/>
    <dbReference type="UniPathway" id="UPA00046">
        <property type="reaction ID" value="UER00505"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008743">
        <property type="term" value="F:L-threonine 3-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019518">
        <property type="term" value="P:L-threonine catabolic process to glycine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
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    <dbReference type="GO" id="GO:0051289">
        <property type="term" value="P:protein homotetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05281">
        <property type="entry name" value="TDH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sorbitol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00627">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
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        <property type="entry name" value="L-Threonine_3-DHase"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
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        <property type="entry name" value="Zn_alcohol_dh"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003808">
        <property type="entry name" value="PRK05396.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="tdh"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401:SF2">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0520">NAD</keyword>
    <keyword evidence="5 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="12">
        <location>
            <begin position="16"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="10">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="10">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="67"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29105"/>
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    <feature type="binding site" evidence="10">
        <location>
            <position position="68"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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    <feature type="binding site" evidence="10">
        <location>
            <position position="97"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
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    <feature type="binding site" evidence="10">
        <location>
            <position position="100"/>
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        <ligand>
            <name>Zn(2+)</name>
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        <location>
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        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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    <feature type="binding site" evidence="10">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
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    <feature type="binding site" evidence="10">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
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    <feature type="binding site" evidence="10">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
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    <feature type="binding site" evidence="10">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
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    <feature type="binding site" evidence="10">
        <location>
            <begin position="266"/>
            <end position="268"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
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        <location>
            <begin position="291"/>
            <end position="292"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity for the proton relay mechanism but does not participate directly in the coordination of zinc atom" evidence="10">
        <location>
            <position position="152"/>
        </location>
    </feature>
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        <source>
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        </source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        </source>
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        <source>
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        </source>
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        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="350" mass="38011" checksum="CC16B06727767B97" modified="2017-09-27" version="1">MAEKMPAIMKTKPAYGAELVEVDVPKPGPGEVLIKVLATSICGTDLHIYEWNEWAQSRIKPPQIMGHEVAGEVVEVGPGVDTLQEGDYISVETHIVCGKCYACKHNRYHVCQNTKIFGVDMDGVFAEYAIVPAQNAWKNPKDMPPEYASLQEPLGNAVDTVLAGPIAGRSTLITGAGPLGLLGIAVAKASGAYPVIVSEPSEFRRELAKKVGADYVINPFEEDPVKAVMDITDGAGVEVFLEFSGAPKALEQGLKATTPGGRVSLLGLFPRDVTLDFNNLMIFKALEVHGITGRHLWETWYTVSSLIQSGKLNLDPVITHKYKGFDKFEEAFELMRAGKTGKVVFFPHKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7T6</accession>
    <name>A0A218P7T6_9EURY</name>
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        <submittedName>
            <fullName evidence="2">Septum site-determining protein MinD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05685</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:ATP hydrolysis activity"/>
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    </dbReference>
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        <property type="term" value="P:negative regulation of cell division"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR050625">
        <property type="entry name" value="ParA/MinD_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384:SF10">
        <property type="entry name" value="ATPASE INVOLVED IN CHROMOSOME PARTITIONING, PARA_MIND FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384">
        <property type="entry name" value="SEPTUM SITE-DETERMINING PROTEIN MIND HOMOLOG, CHLOROPLASTIC-RELATED"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="1">
        <location>
            <begin position="4"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="247" mass="27653" checksum="9DF1F604B6C0EF64" modified="2017-09-27" version="1">MVLIVVTGRGGAGKTTTTANLSTFLAMREYRVLAVDGDLYLPNLGFHFGFDTVKYTLHSVLKNPDIDPEWAIYRHPQTGVHVMPGSTQLQDVLGISPRRLVDILERVKYKFGVIFVDSPTGIPFDTLPTFEVANYQLIVVEIERSPIYSFETMVENEIEKLKALGERYNLNIGVILNKVRESGDVVDKVIEAVEEDLNVPVIGWIPFDNNVPESINVGVPILKYRPKSDAAIAFQETGEVLEEWIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7W5</accession>
    <name>A0A218P7W5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Saccharopine dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_05835</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
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    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051168">
        <property type="entry name" value="AASS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032095">
        <property type="entry name" value="Sacchrp_dh-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133:SF22">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16653">
        <property type="entry name" value="Sacchrp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="2">
        <location>
            <begin position="3"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Saccharopine dehydrogenase-like C-terminal" evidence="3">
        <location>
            <begin position="117"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="362" mass="40964" checksum="4AA1C96842D85A7F" modified="2017-09-27" version="1">MKVLVLGAGNVGRAIAWDLRDEFEVYVGDVSDDRLKTVSEFATPLKVDASDFDELVEAMKNFELVVGALPGRFGYQAVRAAIKAGVDMVDVSFMPENPLELRDEAEKAQVTVIFDAGFAPGLSHMLMGRIWQEMDELKEGYIYVGGLPKEPRPPLYYRITWSPKDLIEEYTRPARVIREGEVRAVDPFERMERVEVRDLEFEAFVSDGLRSLLESVKAEKLEEWTLRWPGHLEKMKVLRELGFFKPEHVDKTLEVITPLMTYESPDFSVMKVIGRGTIDGEPKEIGYLLYDEERDGFTSMARVTGFTAAIVARLVAEQNCIYGVIPPEILGMRIDTFTRIGDELADRGITLERWENAPPGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P813</accession>
    <name>A0A218P813_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="14">NADH-quinone oxidoreductase subunit I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">A3L08_06170</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854185.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P813"/>
    <dbReference type="GeneID" id="33315837"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06170"/>
    <dbReference type="OrthoDB" id="23478at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3270:FF:000014">
        <property type="entry name" value="NADH dehydrogenase subunit I (NuoI)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010226">
        <property type="entry name" value="NADH_quinone_OxRdtase_chainI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004543">
        <property type="entry name" value="PRK05888.2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 8, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849:SF24">
        <property type="entry name" value="NADH-QUINONE OXIDOREDUCTASE SUBUNIT I 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0874">Quinone</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="10" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="13">
        <location>
            <begin position="52"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="13">
        <location>
            <begin position="87"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="186"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="195"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ06936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="210" mass="24961" checksum="CE91AC5923ECDF3A" modified="2017-09-27" version="1">MEEITFKIAQESKIRKKPSFLKPWMSLKYLFKKPVTIKIPYETIEPAKNYRGFHTLNWKTCIGCNMCGQICPARAIEMTWIEGQKRPRPKIDYGRCTFCQFCVEVCPTNALGFVESYMLTTEWKEEELQLFDWVPIPPEKFREIQETYRDWKFPVERMKFNKETKEVTYYLRDGTEFKFKILGYGLKPPAAAKPKPAEKKEEPKAEEKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P817</accession>
    <name>A0A218P817_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF996 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854184.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P817"/>
    <dbReference type="GeneID" id="33315836"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06165"/>
    <dbReference type="OrthoDB" id="86307at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010397">
        <property type="entry name" value="DUF996"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06195">
        <property type="entry name" value="DUF996"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="196" mass="20971" checksum="45DFDC9C7D6DAF88" modified="2017-09-27" version="1">MTVNLRSERNLGLIGSVLVLVGGFLGIIPYIGTFLGTASLVGQILILISLHGIGNKLGDDRPFRYYLYSIVVVIGALIIAAVMALVGILTVPSFVNNGDWTGLVGVSIIGMAVLLLLAALIIGIYFDIKAWRATYEITGVEEFDKVATWTMWGAITLIILVGAILLLVATIYQIIAFANLPEELEPKEKAISSPIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P849</accession>
    <name>A0A218P849_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aldo/keto reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854151.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P849"/>
    <dbReference type="GeneID" id="33315796"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05960"/>
    <dbReference type="OrthoDB" id="275427at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19072">
        <property type="entry name" value="AKR_AKR3F1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43638:SF3">
        <property type="entry name" value="ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43638">
        <property type="entry name" value="OXIDOREDUCTASE, ALDO/KETO REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="1">
        <location>
            <begin position="20"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="278" mass="31698" checksum="3F09AC3C62498EDE" modified="2017-09-27" version="1">MKHVPIFDDLKRIGDDRVTAIGMGTWGIGGYESPDYSRDKESVEVLMHGLELGINLIDTAEFYGAGHSEELVGEAIKDFEREEIFIISKVWPTHFGYESAKKAARASTKRLGTYIDLYLLHWPVEDFRRIEETLHALEELVDEGLIRYIGVSNFDLELLKRSQEAMKKYEIVANEVKYSLKDRWPETSGLLDYMKKEKMALIAYTPLEKGTLARNECLAEIGKAYGKTAAQVALNYLIWEENVVAIPKAGSKAHLEENFGAMGWRLSKEDREKARRCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P859</accession>
    <name>A0A218P859_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Class III signal peptide-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854217.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P859"/>
    <dbReference type="GeneID" id="33315871"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06340"/>
    <dbReference type="OrthoDB" id="86113at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007166">
        <property type="entry name" value="Class3_signal_pept_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04021">
        <property type="entry name" value="Class_IIIsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="61" mass="6513" checksum="EF496503DBEDA0D9" modified="2017-09-27" version="1">MKRKAQGAIEYLFMIAAALIIIAIVIRYLRGAGESTGSTINQGQSAINSKLSQELSEALSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P876</accession>
    <name>A0A218P876_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854180.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P876"/>
    <dbReference type="GeneID" id="33315831"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06140"/>
    <dbReference type="OrthoDB" id="98447at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="270" mass="28787" checksum="7ED33529B075F401" modified="2017-09-27" version="1">MKRALVTLTPPESKRLIAKAVAAMPEVQHALKHGFVYVATGTTAAYVAEELLGEKIEKEKWTVGIISKGRTCVTPKATWPKHLVLYKGEPFDDPLEALKRMGPKDVFIKGANAIDINWNVAVFAAAPDGGTIGKTFGWTITKGVFTITPVSLEKFVPTPVEESAKLTGIYSFDWGTGLYAALVPIPHAHPVTEVEAYRILAGVEAIPIAAGGAGGAEGSVTLVLEGEDEAMEKAKEITKSIKGEPYLKPYTEDCSICPFANICGLGGGDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P877</accession>
    <name>A0A218P877_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_06445</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854236.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P877"/>
    <dbReference type="GeneID" id="33315892"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06445"/>
    <dbReference type="OrthoDB" id="18492at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03217">
        <property type="entry name" value="ABC_FeS_Assembly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010230">
        <property type="entry name" value="FeS-cluster_ATPase_SufC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01978">
        <property type="entry name" value="sufC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43204">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43204:SF1">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="2"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="246" mass="27236" checksum="80450E2C05C68E7A" modified="2017-09-27" version="1">MLKVENLHVSVEDKGILKGVNLSVNPGEFHVVMGPNGSGKSTLALTIAGHPKYKVTDGKITFGGEEIHELGPDERAKKGILLAFQVPPEVEGVKIIEFLQQVLVELRGIDPAEAYNLIVEKAKELWFKEEDLHRYVNVGFSGGERKRLELLQALLIEPKLLILDEPDSGVDVDSLSIISRKIEELHKRGTAILLITHYGRILGHLDREKLTVHVMKDGRIVKTGSGDLVDRIDKEGFAKLFEEVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P890</accession>
    <name>A0A218P890_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06475</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P890"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06475"/>
    <dbReference type="OrthoDB" id="90469at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="69" mass="8329" checksum="924230C36CDFF992" modified="2017-09-27" version="1">MAEELVDITIHVKVPREYAEEFKRDVEARAWYLSHKGELFKNLKKLKGILKTDKSWKELKEEYYEGLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8A9</accession>
    <name>A0A218P8A9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Inosine-5-monophosphate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_06620</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854268.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8A9"/>
    <dbReference type="GeneID" id="33315927"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06620"/>
    <dbReference type="OrthoDB" id="30763at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017158">
        <property type="entry name" value="Tscrpt-reg_CBS-contain_prd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF4">
        <property type="entry name" value="CRO-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037253">
        <property type="entry name" value="HTH_CBS_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="3">
        <location>
            <begin position="13"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="74"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="190" mass="21687" checksum="C7F06DAF864F1A98" modified="2017-09-27" version="1">MVAIPRPIDPREIRRIRKELGITQEELAERAGVTQAYIAKLETGKVDPRLSTFNRILQALLECKKTQLTARDVMSSPVLSVKPYETIENVIKLMNKHNISQIPVIAGNKVVGSVTEKTLVRQSLEYEDIYDHKVMEVMEEPFPIVSEDEDLEVVKYLLEEHPAVLVQDREGRIVGIITRVDIFRIGRGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8D1</accession>
    <name>A0A218P8D1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AAA family ATPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06675</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8D1"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06675"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13238">
        <property type="entry name" value="AAA_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="65" mass="6957" checksum="ED9C00BA83E3D02C" modified="2017-09-27" version="1">MIIVVTGTPGVGKTTISKLLAERLGCRYAGIKESAIERGVKKRAGIVDWSDAYDEVLPYSMLGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8H2</accession>
    <name>A0A218P8H2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">prolyl oligopeptidase</fullName>
            <ecNumber evidence="2">3.4.21.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_07010</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of Pro-|-Xaa &gt;&gt; Ala-|-Xaa in oligopeptides.</text>
            <dbReference type="EC" id="3.4.21.26"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.21.26" evidence="2"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854339.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8H2"/>
    <dbReference type="GeneID" id="33316005"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07010"/>
    <dbReference type="OrthoDB" id="31240at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070012">
        <property type="term" value="F:oligopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.120">
        <property type="entry name" value="Prolyl oligopeptidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023302">
        <property type="entry name" value="Pept_S9A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002470">
        <property type="entry name" value="Peptidase_S9A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051167">
        <property type="entry name" value="Prolyl_oligopep/macrocyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42881">
        <property type="entry name" value="PROLYL ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42881:SF2">
        <property type="entry name" value="PROLYL ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02897">
        <property type="entry name" value="Peptidase_S9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00862">
        <property type="entry name" value="PROLIGOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50993">
        <property type="entry name" value="Peptidase/esterase 'gauge' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S9A N-terminal" evidence="7">
        <location>
            <begin position="2"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="6">
        <location>
            <begin position="416"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="617" mass="70158" checksum="585576294C927A03" modified="2017-09-27" version="1">MEDPYIWMENLNDERVLKLVEEENKRFREFIGDLSDELFPEVWEYYSMPTLHGARLTDKGIIAMYKERDRQVIRWLGGDVIIDSKELEKELNDEVLLQGFTADKSGKLLAYSFSIGGADEGITRIVNLETGEILEEFKPSVWNVTFLENGYYFSRFYRHGETPDGVKAPAVRLFWKDENGERMVFGKGLGSGYFMGLRKSTDGKWAMLTVTFGWNKADIYIGPIDAPDLWGKVYSAEVPAEPIDVVNGRLYVLTREGKGRGKVIAIEGENITEVIPEGEFPLEWAVIVGDKILAGRLVHASHRLEVYSLSGEKLDEVTFDLPGSVYPLDTDGKRVLLRYESFTVPYRLYEFDGELKLVERQEVEGNFRVEEDFAVSKDGTKIHYFHVKGTEDEKKVWVFGYGGFNISLTPRFFPQAIPFIKRGGSFAMANLRGGSEYGEEWHRAGMRENKQNVFDDFIAVLSKLKGEGYRVAAWGRSNGGLLVSATLTQRPDVMDSALIGYPVIDMLRFHKLYIGSVWVPEYGNPDDPKDRAFLLKYSPYHNIDPNKKYPPTLIYTGLHDDRVHPAHALKFFMKMKEIGAPVYLRVETKSGHMGASPETRARELTDLLAFVVKTLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8H9</accession>
    <name>A0A218P8H9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Peptide ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_06950</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854328.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8H9"/>
    <dbReference type="GeneID" id="33315993"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06950"/>
    <dbReference type="OrthoDB" id="312811at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025966">
        <property type="entry name" value="OppC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42729">
        <property type="entry name" value="OLIGO/DIPEPTIDE TRANSPORT, PERMEASE PROTEIN (DPPC-2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42729:SF1">
        <property type="entry name" value="OLIGO_DIPEPTIDE TRANSPORT, PERMEASE PROTEIN (DPPC-2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12911">
        <property type="entry name" value="OppC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="74"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="282" mass="31101" checksum="42F487FBF0AF8699" modified="2017-09-27" version="1">MNKEIFYVLFRNKRFVIGFSILVFEILLSLFGAYLYPVDPFSPAGPPASPPNSYYLLGTDQFGRDILAQVVVGIRNSLYVGVLTGIFSIAIGLTIGTIAGIKGGLIDESLMAFTNVVLTIPNVLLAIIIATYMGLENAGLTLVAVIISLTAWPWFARAIRAQFLSLRERDFVHFSRMTGYGDLKIAVFDLLPYVTTYTLVSFVNFMNIGINAEVGLSILGLTPIHIMTLGKMLYFAAVTQSYFLGHWWVFIPPGILLVALSTSLLLIATGIEQVFNPRLREM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8I4</accession>
    <name>A0A218P8I4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Solute-binding protein family 5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_07085</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854351.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8I4"/>
    <dbReference type="GeneID" id="33316020"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07085"/>
    <dbReference type="OrthoDB" id="194307at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904680">
        <property type="term" value="F:peptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.105.10">
        <property type="entry name" value="Dipeptide-binding Protein, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290:SF9">
        <property type="entry name" value="OLIGOPEPTIDE-BINDING PROTEIN APPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PERIPLASMIC BINDING COMPONENT OF ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="769"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="domain" description="Solute-binding protein family 5" evidence="6">
        <location>
            <begin position="74"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="740"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="792" mass="89715" checksum="7F01BEBE318B6694" modified="2017-09-27" version="1">MRRFFLALLLSFLLVMPSVQALDPKASIIEFQGAQYLERVTRQVAEGQYDIGLFSLPMEDYLLFGGDVLKSLDLYSRTVSYNELTFNTYHNPDKDAPLVTVGGNVYFNPFAVREVRFALNYLIDRNYIVREIYKGGAAPMFGCVGPSHPADRYFEPVYSALNLSDSQNAERAIELFNEGMGKAAQAVSRYNHTLEKVNGTWYFDGEPVTVKSVILIEDERQKIGRYVVDLIERYFGFRVERLEWDRQKAGQVVFATSPREYEWNLYTGGWNVRLSEVPSVWVDDYTARFYAAWYGYLPGKDEPKHRNTVTVGEFFRYVGNGDADEGLRAIGAEYYKSTSELRGILNWTEEELTKLLVRFSLETDGENYTLESAEQYWDLQKISIGLGIMESARVFLTEVWELSPVNRERVFGIDADPNVGLFNRWSLLSAETPDGVLRVAYFVPTCGCTCLPFNPAVESRYPVFPIDLWDLLHDPAGYTDQNGIYTPYRCMWSVERGNFTVPEDAVIYNQTRGWIPLHAGKTARIRVRVSCDLGEWQNGIKMRTADIKYYIAFLYTWAYEDVPNDLYYEDDLSDTANSLRNVLGFEFRDDGYVVYGSYSHPIADDLTAKEYVFYPKSPWELYYAMGELVANGSAYGIYEKYSLGNAEGRVECLNLLTKEYVADLRRILTSLKDRGAIPPAIAGEVSDPEEGYARLIKWIDAFGNAVISDGPFYIEKNIPENLFMGLRAFNGSSPIAPPNITTTAPVSASPKSSPIHQSGGEPETSPGTWLPYAIGLGVLLILVLVFLKGRKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8K3</accession>
    <name>A0A218P8K3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphoribosylaminoimidazole-succinocarboxamide synthase</fullName>
            <ecNumber evidence="6">6.3.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">SAICAR synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">purC</name>
        <name evidence="8" type="ORF">A3L08_06905</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate + ATP = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:22628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SAICAR synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.6" evidence="6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854320.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8K3"/>
    <dbReference type="GeneID" id="33315984"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06905"/>
    <dbReference type="OrthoDB" id="10775at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00131"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00137">
        <property type="entry name" value="SAICAR_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028923">
        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050089">
        <property type="entry name" value="SAICAR_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018236">
        <property type="entry name" value="SAICAR_synthetase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43599">
        <property type="entry name" value="MULTIFUNCTIONAL PROTEIN ADE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43599:SF3">
        <property type="entry name" value="SI:DKEY-6E2.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01259">
        <property type="entry name" value="SAICAR_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01058">
        <property type="entry name" value="SAICAR_SYNTHETASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAICAR synthetase/ADE2 N-terminal" evidence="7">
        <location>
            <begin position="3"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="219" mass="24989" checksum="8F9A169721FBA641" modified="2017-09-27" version="1">MRLLYSGKTKDVYEDGPYLIFHFKDTVLGRNGEEDSGGNEVVGEKAGKGSVVLDQTEFFFKLLEKNGVKTHFVERIDGRKARLLKAERIPLEVIYRELAYGSFLRRYNGWVRPFQWLRIVEFTLKDDRLDDPVIAEDAISSLGIASREEVEEMKRTTVKVAEILRGFLASKGLQLVDFKLEFGRLNGELLVIDELSGDTMRVAKDGRILTREELLEVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8R0</accession>
    <name>A0A218P8R0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cyclic 2,3-diphosphoglycerate synthetase</fullName>
            <shortName evidence="5">cDPGS</shortName>
            <ecNumber evidence="5">6.5.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cpgS</name>
        <name evidence="6" type="ORF">A3L08_07430</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the formation of cyclic 2,3-diphosphoglycerate (cDPG) by formation of an intramolecular phosphoanhydride bond at the expense of ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(2R)-2,3-bisphosphoglycerate + ATP + H(+) = cyclic (2R)-2,3-bisphosphoglycerate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:42412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58248"/>
            <dbReference type="ChEBI" id="CHEBI:79081"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.5.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cyclic 2,3-diphosphoglycerate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.9" evidence="5"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854414.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8R0"/>
    <dbReference type="GeneID" id="33316089"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07430"/>
    <dbReference type="OrthoDB" id="85545at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036356">
        <property type="term" value="F:cyclic 2,3-diphosphoglycerate synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01908">
        <property type="entry name" value="Cyc_PG_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016557">
        <property type="entry name" value="Cyc_diphosphoglycerate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009445">
        <property type="entry name" value="Cyc_PG_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="436" mass="47803" checksum="0073D7138B023824" modified="2017-09-27" version="1">MRVALVDGEHYPDVVKWALERLGDVCCAVFLGGSEKIGSLEQVKMRLGIKVYYDPSDYLSALSRALRENPGIEEVVDLSDEPVLNYEDRFRIASLCMLHGVVYRGADFEFRPRPLKKTKKPSIAIIGTGKRVGKTAVSGFVARTLKEIANPVIVTMGRGGPEEPELIKGEEFEITPEFLLKLSESGRHAASDHFEDALTSRVTTIGCRRCGGGMAGFPFFDVVDKGVELAESLPNNLILLEGSGATFPAYRADGYITVVSALQKLEFIGDYFGPFRISLADIVVITMADLTDEGKLEALKRAILDINPSADLHITAFRPRPLETVEGKRLGLVMTSETALETARRHLENLGAEVMAMSGNLSRRPALRKDLEKFRGIDAVAVELKAAAVDVVTRWALERGIEVIYLDNEPVNLDGKDLRESVLKLGRKVLSRGSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8T4</accession>
    <name>A0A218P8T4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_07595</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854443.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8T4"/>
    <dbReference type="GeneID" id="33316122"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07595"/>
    <dbReference type="OrthoDB" id="98202at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="174" mass="20279" checksum="760A87BCFECCBD70" modified="2017-09-27" version="1">MYEVKKSRAGYIFDLPRERIAFMFLKDGTYLMYHDEKTLCYSMKPVDVSKEELEHFERTGELPEIIKAIKSGSYPESCVVKELPPIDEDLKPLNPSRKCVVIFTGFQDTVIDYVECENEILAVARLVDEPEKVCRFFGRGNYKIAAVKLKRGEKCLTREEFLKKVEECMERLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8T7</accession>
    <name>A0A218P8T7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endonuclease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_07685</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854457.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8T7"/>
    <dbReference type="GeneID" id="33316140"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07685"/>
    <dbReference type="OrthoDB" id="99512at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="15920" checksum="FA624E31788F5161" modified="2017-09-27" version="1">MDILFDFEPETVRDEEDLEKQLYQYLKARLGSRVTRQYPAGDQKIDIAVDGRVGIEIKIAESRSKLQRLAGQVLDYVEYFDEVIAVILDVGANVDIDNYIKKLRQLGAEVVVLEGDIKRKGRSREIIIKDARRKIIIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P900</accession>
    <name>A0A218P900_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Restriction endonuclease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_08055</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P900"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08055"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019292">
        <property type="entry name" value="McrC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38733">
        <property type="entry name" value="PROTEIN MCRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38733:SF1">
        <property type="entry name" value="TYPE IV METHYL-DIRECTED RESTRICTION ENZYME ECOKMCRBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10117">
        <property type="entry name" value="McrBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="413" mass="48806" checksum="8732EA3C8A3D2048" modified="2017-09-27" version="1">MNLRIDLKEWQDISEIPEFKDNPFIPIRSIPPDVKKFIDIVDISPRGVKIKARDYVGVFPLSDDIILTVSPKAPVEDFLYMFYKAQGIKLDIKDIKKIAQAGRKYGIEHPNVFHFLITALLAELEQLKRLGFLKTSQHVTQDKIIKGKVLTKETINEWLRGNQQKVVCEKFELSKDNIVNSSIKFTLWSLINMYSNLLGDEIKNELFKKYLWFKDVSLPKNMSFIEEIEKILTFRALPNSRSYYYSILNLCMFFIANSTLEFRSPKRFKVRAFAVYMNKVFENYLYAVLKEQLGYEYRVKLHPTQQLFDDNNKYSLELDYLILKDDKPVLVVDAKYKSKPVTDDFYQILLYAEKFGIKNSLLIYPSWGKRTSTEVFRFKERQVHVMYYDLSDIKKSESTLREYIYCLVNLKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P914</accession>
    <name>A0A218P914_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA methylase adenine-specific domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_08040</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P914"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08040"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003356">
        <property type="entry name" value="DNA_methylase_A-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052916">
        <property type="entry name" value="Type-I_RE_MTase_Subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42998:SF1">
        <property type="entry name" value="TYPE I RESTRICTION ENZYME HINDI METHYLASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42998">
        <property type="entry name" value="TYPE I RESTRICTION ENZYME HINDVIIP M PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02384">
        <property type="entry name" value="N6_Mtase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00507">
        <property type="entry name" value="N12N6MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0680">Restriction system</keyword>
    <feature type="domain" description="DNA methylase adenine-specific" evidence="3">
        <location>
            <begin position="131"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="DNA methylase adenine-specific" evidence="3">
        <location>
            <begin position="581"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="267"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="746" mass="86844" checksum="8309978DA471B2CB" modified="2017-09-27" version="1">MSAHSNNAQSSLIIEIQKVHDFLYKELNDTKILLPLMLTVLDKYKEKKSNESEDPIVELKKDLEKLDRRMMEQTLQKIKREHSNINPLLERLINESLNAISPERLPEAKIERVKQRLLRADKFFEGLEREEDFIGLLFETLAAHSGVFDKKEGRFFTPKNIILTVVEMIGSIFEAKDVDVTKVSVCDPCCGSGRYLVHWANRIKREYEISDMELKKVYSDNLFGIDISPEVASWATWNMIFHGDGATNIENADSLNWYGFLVHWNLIQKLLEELPSELQKIKRIRQRDVGEAINELKEMAQIVDDLKEKNEIDLESEEVKTLFRLLDKLISLHHKYNIKWPTLEELSKRRDFKSISEIVKLKWVKEHPHIKNGFDLIITNPPFGRGSQDLMVTDPYILCQYKLATELWLGDISKELAEKLIEKQFEMDVVDFYRKSLKELKIRETREEYEIKFNDLPSKLLKRLAQKHGIPTKGKSKKEIVDALKDKLNREILIDGELSLSIDSLPDSFIKKLASRILYDKVGGKSKVGEFLYSKIQEKLGREWITVEDVYDYSTKLTIKDSATGDEHTIYYNEDGEPILFKEALPKQVVFLEEFLRFVKEGGYVFTVVDTGVLSNSEDEYVRRFLYRNSRIHAIVEFPHNAFKAAGTGVKTAVILYQKLRNPPEDYEIFGALPQHLGYVLNKQDTPPDPDHNDLGKVLCDWRFYLGLGRMCEPCKDPNSKIEECCDWYKKGYCPVWREKIERADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P927</accession>
    <name>A0A218P927_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MATE family efflux transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_08160</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854540.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P927"/>
    <dbReference type="GeneID" id="33316238"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08160"/>
    <dbReference type="OrthoDB" id="214119at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13147">
        <property type="entry name" value="MATE_MJ0709_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048279">
        <property type="entry name" value="MdtK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052031">
        <property type="entry name" value="Membrane_Transporter-Flippase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43549:SF2">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN NORM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43549">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN YPNP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="283"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="320"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="369"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="396"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="420"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="458" mass="48928" checksum="BF528D6259E99747" modified="2017-09-27" version="1">MGEKVTKGVQILRGDPKKAIIKLSIPMMIGMSVQTVYNLADGIWVSGLGPEALAAVGLFFPVFMGIIALSSGLGIGASSAIARRIGAKDKKGADNAAIHSLILALILGILLTVTMLPIIGQILTAMGAKGRAVELAIEYSRVLLIGAVVMVFNNVGNGILRGEGDANRAMYAMVLGSGLNIVLDPVFIYTLGFGVVGAAYATLLSMIVTSLFIAYWLFIKRDTYVDITLRDFSPSKEVLKDILRVGIPSSLAQLTMSIAMFFLNRIAIMAGGENGVAVFTSAWRVTMLGIVPILGMASATTAVTGAAYGERNIQKLETAYLYAIKLAFMIELGVVAFIMVFAPQISYLFTYSESALVIKEGLIEALRTLPIFLVLTPFGMMTSAMFQGIGEGEKSLVLTIFRTVVMQVGFAYLFANYTGFGLIGVWMGIVLGNMVAAVVGFTWGRLRVKQLKEAFGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P935</accession>
    <name>A0A218P935_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Indole-3-glycerol phosphate synthase</fullName>
            <shortName evidence="11">IGPS</shortName>
            <ecNumber evidence="4 11">4.1.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">trpC</name>
        <name evidence="13" type="ORF">A3L08_08115</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+) = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23476"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="EC" id="4.1.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the TrpC family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.48" evidence="4 11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854533.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P935"/>
    <dbReference type="GeneID" id="33316228"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08115"/>
    <dbReference type="OrthoDB" id="15223at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00043"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004425">
        <property type="term" value="F:indole-3-glycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00331">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00134_A">
        <property type="entry name" value="IGPS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045186">
        <property type="entry name" value="Indole-3-glycerol_P_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013798">
        <property type="entry name" value="Indole-3-glycerol_P_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001468">
        <property type="entry name" value="Indole-3-GlycerolPSynthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001376">
        <property type="entry name" value="PRK00278.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22854:SF2">
        <property type="entry name" value="INDOLE-3-GLYCEROL-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22854">
        <property type="entry name" value="TRYPTOPHAN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00218">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00614">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="10 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Indole-3-glycerol phosphate synthase" evidence="12">
        <location>
            <begin position="9"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ07286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="235" mass="26524" checksum="33C47B435E90BAB6" modified="2017-09-27" version="1">MRVFGLSRAIRKSKKNAVIAELKVYSPKYGDLLNGRDPFEILRAYERAGAVGISYITEPQYFHGSFEFLKRLCRETELPVLRKDFITTKEEIERTAEAEASAVLLIARLLGESLGEFVDYAKEHGLDSLVEVHSRKELSLAVETETTMIGINNRDIRKLELDDGNVSLTEELAPLIPRRYVRVSESGISSVEDLRRALKHADAALIDTALMKVDDPEKFLRQLRGGEVMSLLEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P977</accession>
    <name>A0A218P977_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pyruvate carboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_08425</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854592.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P977"/>
    <dbReference type="GeneID" id="33316291"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08425"/>
    <dbReference type="OrthoDB" id="6555at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004736">
        <property type="term" value="F:pyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07937">
        <property type="entry name" value="DRE_TIM_PC_TC_5S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000003">
        <property type="entry name" value="Acetyl-CoA carboxylase biotin carboxyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003379">
        <property type="entry name" value="Carboxylase_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055268">
        <property type="entry name" value="PCB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006761">
        <property type="entry name" value="PRK09282.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43778">
        <property type="entry name" value="PYRUVATE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43778:SF2">
        <property type="entry name" value="PYRUVATE CARBOXYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02436">
        <property type="entry name" value="PYC_OADA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89000">
        <property type="entry name" value="post-HMGL domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0092">Biotin</keyword>
    <keyword evidence="5" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="4">
        <location>
            <begin position="4"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="3">
        <location>
            <begin position="527"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="605" mass="66976" checksum="27D218AF2C65D480" modified="2017-09-27" version="1">MARVEIIDTTFRDAHQSLIATRLQTEDMLAIAEKMDRIGFYSMEVWGGATFDVCIRYLNEDPWERLRLLREHIKKTKLQMLLRGQNVVGYKHYPDDVVGRFVELAHKNGIDIFRVFDALNDVRNMEVAIRKAKEVGAEVQGAIAYTTGKVFTLEYYMKKVEELLALDVDVITIKDMAGLLTPRKAYELVSEIKETYGVPVNVHTHSTTGMAVATYLKAVEAGADFIDTAISPLAFGTAQPGIQTIWHALPEAVGSHLDRELIHEVSRYLKKLLDEKYSGLLHKEALMVNPYVLKYQVPGGMYSNLISQLKEMKALDRLQEVLEEIPRVREDLGWPPLVTPTSQIVGTQAVLNVLFGRYERITEEVKNYIKGLYGRPPAEVNPELRKRVLGDDEPITVRPGELLEPMLETCREELEKLGYLEKEEDVLTYCLFPQIAKEFFERRKRGIKTPSMSPSAQTFKLYVNGVEFEVGVEGVDLSALKYLPQIASSAQAPTTAPSTGTALVSVPSVPTAAPTPAPAPVAPVVPAAPTPAPAGEGVVTAPMPGKILRILVKEGDSVKTGQGLLVLEAMKMENEIPAPKDGVVKKILVKEGDTVDTGQALMELG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9A7</accession>
    <name>A0A218P9A7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7 8">Prefoldin subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">GimC subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pfdA</name>
        <name evidence="10" type="ORF">A3L08_08470</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 7">Molecular chaperone capable of stabilizing a range of proteins. Seems to fulfill an ATP-independent, HSP70-like function in archaeal de novo protein folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7">Heterohexamer of two alpha and four beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the prefoldin alpha subunit family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854600.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9A7"/>
    <dbReference type="GeneID" id="33316300"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08470"/>
    <dbReference type="OrthoDB" id="10045at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23160">
        <property type="entry name" value="Prefoldin_alpha_GimC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00308">
        <property type="entry name" value="PfdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011599">
        <property type="entry name" value="PFD_alpha_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00293">
        <property type="entry name" value="prefoldin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12674">
        <property type="entry name" value="PREFOLDIN SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12674:SF2">
        <property type="entry name" value="PREFOLDIN SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="104"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="NCBIfam" id="TIGR00293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ07353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="147" mass="16366" checksum="C3B7219AA8D7155D" modified="2017-09-27" version="1">MAETNEQLERLAYEYQLLQAQAQLLAQNLELLTLGRNEFQAVKETLEELKKVEDEKPEILVPIGAGSFLKGVIVDKENAIVSVGAGYAVEKSLDDAIAYIDARIREYEEAIAKTQDGLKKLEAQLGELAQKAQELQQRQAMGFSVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9C9</accession>
    <name>A0A218P9C9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Rubrerythrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_08725</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854641.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9C9"/>
    <dbReference type="GeneID" id="33316351"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08725"/>
    <dbReference type="OrthoDB" id="86289at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01045">
        <property type="entry name" value="Ferritin_like_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008553">
        <property type="entry name" value="DUF835"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rr_diiron-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33531:SF10">
        <property type="entry name" value="BLR7895 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33531">
        <property type="entry name" value="RUBRERYTHRIN SUBFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05763">
        <property type="entry name" value="DUF835"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rubrerythrin diiron-binding" evidence="1">
        <location>
            <begin position="25"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="DUF835" evidence="2">
        <location>
            <begin position="183"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="324" mass="37437" checksum="59544FA46D2D2BFD" modified="2017-09-27" version="1">MENNTGATQALEEIVEALRNKSPKELLSYAIFNEEDEAEYYAELARRVSMVSVKVLFLKMSEESNNHREWLYHLFKKLYPNEEPVKVEAPPVEVAPFLQEFETVDDYVSALEYCMESELFAKKTYEILAKVAEDEDTRALALHLATMEEEHYHEIRRLYELITLMKERDINPPHLDPGGYLFTDDTKAKYFLLDMLDSNRVLKALVRENPEQFMKLFEGKDASAVWVTKTGAENSIPPSGVPTLRKDLFRFFEKASRDGKQGIVFIQNFGYLVLELGFKDAIDFALYLKDSALHYNGYVVMTAVPTAFEKKEWSLLTSEFVEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9D4</accession>
    <name>A0A218P9D4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MFS transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_08635</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854625.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9D4"/>
    <dbReference type="GeneID" id="33316333"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08635"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17504">
        <property type="entry name" value="MFS_MMR_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF9">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="233"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="271"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="306"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="339"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="413"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="452"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="17"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="496" mass="53836" checksum="D2BA2CBDDA94AC4B" modified="2017-09-27" version="1">MNATKNAEYDLAYAKKAMLVVVILPLLVMYTEAMLTPALPTIQKEFAINPNDVSWVLTIYLLVGTVSVALFGKLGDMYGKKKMFLVALGFYTLGVILNGFAPSFQWLLVTRGIQGFGMAIFPLAFSLVREEFPPKMVPQVQGMISAMFAVGMVIALPLGAWVTQNWGWRWTYHSAAPFAVLMFFIAWKVLRESRYINPGKVDWVGALLLAFTVVPALVGVTRAPNVGWTARETLVLFVVAVVSAVLLVLWEKRAENPLIPIEIISARNPAIVNIGIMFAAFGISMMSQANTYIFQMPAPYGFGKTILQSGLLMTPMALVMLIVAPIAGKLLPKVGAKPIALTGALTASIGLGILSKYAPQLPPDHLWAFVGLITLVGTGITLMNISLINVLVFSVPPRAMGVATGANSLFRNFGSTWGPAIAGTIMSTYYTVFHPPGAPEWVKIKIPTTKAYEVLFGTSAAIFLLLALLILAIREVMKGGRIHEVENEGEKEVVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9D5</accession>
    <name>A0A218P9D5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA double-strand break repair Rad50 ATPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rad50</name>
        <name evidence="12" type="ORF">A3L08_08755</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. The complex may facilitate opening of the processed DNA ends to aid in the recruitment of HerA and NurA. Rad50 controls the balance between DNA end bridging and DNA resection via ATP-dependent structural rearrangements of the Rad50/Mre11 complex.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per homodimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer. Forms a heterotetramer composed of two Mre11 subunits and two Rad50 subunits.</text>
    </comment>
    <comment type="domain">
        <text evidence="9">The two conserved Cys that bind zinc constitute the zinc-hook, which separates the large intramolecular coiled coil regions. The 2 Cys residues coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another Rad50 molecule, thereby forming a V-shaped homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SMC family. RAD50 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854647.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9D5"/>
    <dbReference type="GeneID" id="33316357"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08755"/>
    <dbReference type="OrthoDB" id="25344at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.510">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00449">
        <property type="entry name" value="RAD50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041685">
        <property type="entry name" value="AAA_GajA/Old/RecF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022982">
        <property type="entry name" value="Rad50_ATPase_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013134">
        <property type="entry name" value="Zn_hook_RAD50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003034">
        <property type="entry name" value="PRK03918.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32114">
        <property type="entry name" value="ABC TRANSPORTER ABCH.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32114:SF2">
        <property type="entry name" value="ABC TRANSPORTER ABCH.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13175">
        <property type="entry name" value="AAA_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75712">
        <property type="entry name" value="Rad50 coiled-coil Zn hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51131">
        <property type="entry name" value="ZN_HOOK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc-hook" evidence="11">
        <location>
            <begin position="391"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="154"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="454"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="614"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="32"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10">
        <location>
            <position position="438"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10">
        <location>
            <position position="441"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="792"/>
            <end position="797"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ07401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="887" mass="103245" checksum="304548A08DF40A48" modified="2017-09-27" version="1">MRIRRIKIKNFRAHEKSEVEFSDGINLLIGQNGAGKSSILEAIFAALYMGHPSFPRGYLQANTRVNAKGGIVLALEFEHDGKTYRITRDTKKSELLEDGALIAEKSSDIARWVERNVYPIQVFTNALYIRQGEIEGIITNREIMEKVLRKVLGIEDYENAEKNAAEVIRELRRKRDYLRKLVERKAEVEESLRDAEKRFSETLRRISELRERARKLEGEFEEAEKEYSRLKALKEELEGLEKRKAIVEQRIRAEKDRIEDYEGQIFEVEREIEELEEKFSRLKELEPLEKEYLKLKSLLSLKDELAKLELSTARLAEKKKTLEEKTSRINEVSSKIAELEKEETALRETYEELKRKNSLHQRALQLKAEAERYRSELEKAGTTPEEIEEGLKSIEKAKEELEKLREEVARTREEMASLTGLKESLIENLSKLEGAKVCPLCKRPIEEHDEEEIKREYDAEIASIEKKLKGLEKKLETLREREPELKETIRKEPKLIRLKKTADLLREVEEKLSKYDLEELEKAAEEFENTKARLIEIKKELRHLREELEELENARKELERIETRLAEIEVEKGKITEKLGGEGFDSFDEVEGRLKELEPAYREYLSLKGVPAQLERARKKLTLLEKRRDESIKTLGELEGELKGLKAEIEKRSREFSEEAYREAEKRYIESARELEKAKAELKGAEELRNEVMKLLDELRAKRKEIEDAEKEIETVEKAIADMTAFKEKVARLKAEEELRGLEEVQKLAGETFSEMTEGKYQGIKLKREKKYGKERIELKVLYAGNEVGLEFLSGGERIALGLAFRLALSLYKVGNLELLILDEPTPFLDEERRKKLVEIISSQLRKIPQVIIVSHDEELKDAADYVIRVENVGGKSRVEVESLGAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9F3</accession>
    <name>A0A218P9F3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NYN domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08865</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854667.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9F3"/>
    <dbReference type="GeneID" id="33316379"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08865"/>
    <dbReference type="OrthoDB" id="85234at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18726">
        <property type="entry name" value="PIN_LabA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002790">
        <property type="entry name" value="CHP00288"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021139">
        <property type="entry name" value="NYN"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00288">
        <property type="entry name" value="TIGR00288 family NYN domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35811:SF1">
        <property type="entry name" value="HTH OST-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35811">
        <property type="entry name" value="SLR1870 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01936">
        <property type="entry name" value="NYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NYN" evidence="1">
        <location>
            <begin position="29"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="167" mass="18380" checksum="38B8FC260E2E77D7" modified="2017-09-27" version="1">MKETLFKVLRRGGREVSEEPVKSVKGKSIGLIIDGPNILRKEFGIKLEDIIEVLERIGKIRVAKVVLNQYAPQGLIEAVVNQGLEPIIVAGDTDVRIAIEAMELIYNSDVEVIALATRDADFLPIINEAKRKGKETIVIGVEPGFSVALQNAADYVIKMEGKGEERE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9J9</accession>
    <name>A0A218P9J9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">fructose-bisphosphatase</fullName>
            <ecNumber evidence="4">3.1.3.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_09090</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the inositol monophosphatase superfamily. FBPase class 4 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="4"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854706.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9J9"/>
    <dbReference type="GeneID" id="33316424"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09090"/>
    <dbReference type="OrthoDB" id="58111at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008934">
        <property type="term" value="F:inositol monophosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006020">
        <property type="term" value="P:inositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000027">
        <property type="entry name" value="Fructose-1,6-bisphosphatase/inositol-1-monophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000020">
        <property type="entry name" value="Fructose-1,6-bisphosphatase/inositol-1-monophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009321">
        <property type="entry name" value="PRK12676.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854">
        <property type="entry name" value="INOSITOL MONOPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854:SF4">
        <property type="entry name" value="INOSITOL-1-MONOPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ07462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="256" mass="28111" checksum="37099E5431526210" modified="2017-09-27" version="1">MEIAWNEIARSLALDVEKVVMPLFGTSRAGETVGMNVSGDVTKYVDKVAEDLVIERLKPLGVNIVSEEIGFIDNGSDYTVVVDPIDGSYNFAAGIPIFAFSFAVFKGKKPVYGSIHEFVPRHFYEAVPGEGAFMDGERIYVRKPERGKEALSFYTRGRCTGLIKRVKRVRVLGAIAVELTYLAKGALDGVLDIRNYVRPTDIAAGVLIAREAGAIVTDERGKELEVHLSATEQTNVIAVNDEYLLKIILEELENGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9K1</accession>
    <name>A0A218P9K1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09065</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854702.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9K1"/>
    <dbReference type="GeneID" id="33316419"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09065"/>
    <dbReference type="OrthoDB" id="85529at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="141" mass="16859" checksum="E5A293AF249DA38C" modified="2017-09-27" version="1">MLLTRHAWERLIKRLAKKRKLERIYAELWDFLDRSRRIDVNEKVVIFTDSRKSLVCARLDCERLSREEIEEALGGIENPYKCVFFDERLVRETVPRKFLELVPDGVYCFYINREKRSIYIGSEPPLLVVTIRPAKKNEREG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9L4</accession>
    <name>A0A218P9L4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854675.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9L4"/>
    <dbReference type="GeneID" id="33316388"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08910"/>
    <dbReference type="OrthoDB" id="101178at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="11717" checksum="3C096DBDD5F97F15" modified="2017-09-27" version="1">MLPELVMGFIFTIVWALSYVLVLKQRSVARALLGVLVLFGAIVLFTPYRFQGNLLGWFIGISAGFFVGLQLVQKYGPEKPTDESAIAVFLLGPLIFALLLILVLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9M0</accession>
    <name>A0A218P9M0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Septum site-determining protein MinD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_09170</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854721.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9M0"/>
    <dbReference type="GeneID" id="33316440"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09170"/>
    <dbReference type="OrthoDB" id="31168at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051782">
        <property type="term" value="P:negative regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000285">
        <property type="entry name" value="Sporulation initiation inhibitor Soj"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010224">
        <property type="entry name" value="MinD_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025501">
        <property type="entry name" value="MinD_FleN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050625">
        <property type="entry name" value="ParA/MinD_ATPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01969">
        <property type="entry name" value="minD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384:SF10">
        <property type="entry name" value="ATPASE INVOLVED IN CHROMOSOME PARTITIONING, PARA_MIND FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384">
        <property type="entry name" value="SEPTUM SITE-DETERMINING PROTEIN MIND HOMOLOG, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003092">
        <property type="entry name" value="MinD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="3"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="12"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR003092-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="259" mass="27470" checksum="1651331EFEDC79B5" modified="2017-09-27" version="1">MEGRSIVFASGKGGTGKTTTVANLGVALAQFGKEVILLDADITMANLSLVLGMEDIPITLHDVLAGEADLKDAIYEGPAGVKVIPGGLSLEKVKKAKPEKLRQLIREIGQMADFVLIDAPAGLEMTSVTALLIGKELIIVTNPEISAITDSLKTKLIAEKLGTLPLGAILNRVTNEKTELTQEEIEAILEVPVLTMIPEDPEVKRASAYGVPLVIKNPTSPAAIAIKQLAAKLAGIKWQAPEPESPIKRVFKAIFGGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9R4</accession>
    <name>A0A218P9R4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Translation initiation factor 6</fullName>
            <shortName evidence="3">aIF-6</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">eif6</name>
        <name evidence="4" type="ORF">A3L08_09495</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Binds to the 50S ribosomal subunit and prevents its association with the 30S ribosomal subunit to form the 70S initiation complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eIF-6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854779.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9R4"/>
    <dbReference type="GeneID" id="33316505"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09495"/>
    <dbReference type="OrthoDB" id="33582at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00527">
        <property type="entry name" value="IF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.75.10.10">
        <property type="entry name" value="L-arginine/glycine Amidinotransferase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00032">
        <property type="entry name" value="eIF_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002769">
        <property type="entry name" value="eIF6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00323">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003129">
        <property type="entry name" value="PRK04046.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10784">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01912">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006413">
        <property type="entry name" value="IF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00654">
        <property type="entry name" value="eIF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55909">
        <property type="entry name" value="Pentein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2 3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="228" mass="24824" checksum="590EF1B85A8DC91F" modified="2017-09-27" version="1">MHIERLDFENSPYLGVYGLATDKVVLVREGLGEKKLAVLREVLKVPLIETSVMKSRIVGIFAAGNSNAIVVPWYIWDAELDAINNQLKEYGIDVEVVPFQSTLTAFGNLILANDKAALISAKFTREEAKKLEDILGVEVERGMIGDYHAVGSAGVVTNRGGLVHPEATDEELEWLRDLFKVDVYVGTANMGVPFVGSCMLANSHGVVVGHLTTGPEIVKIEEALGFLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9U7</accession>
    <name>A0A218P9U7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein tyrosine phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_09600</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854798.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9U7"/>
    <dbReference type="GeneID" id="33316526"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09600"/>
    <dbReference type="OrthoDB" id="117569at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000157">
        <property type="entry name" value="Protein-tyrosine phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050561">
        <property type="entry name" value="PTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23339">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASE AND DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="3">
        <location>
            <begin position="2"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="4">
        <location>
            <begin position="70"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="150" mass="17300" checksum="123B3011654BEA06" modified="2017-09-27" version="1">MWLSARFVDENVAFSRMPSDVELDEVADDFDAIVVLVEDYELPYSLEEWEKRGVEVLHSPILDFTAPSLEQLLEILRWIDARVLEGKRVLIHCMGGLGRSGTVAVAWLMYSKNLRLSEALRRVRSVRPGAVETYEQMEVLKELESFLRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Y6</accession>
    <name>A0A218P9Y6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ASCH domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854821.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Y6"/>
    <dbReference type="GeneID" id="33316551"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09725"/>
    <dbReference type="OrthoDB" id="84912at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06552">
        <property type="entry name" value="ASCH_yqfb_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.30">
        <property type="entry name" value="Hypothetical protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007374">
        <property type="entry name" value="ASCH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42250">
        <property type="entry name" value="ASCH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42250:SF1">
        <property type="entry name" value="ASCH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04266">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01022">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ASCH" evidence="1">
        <location>
            <begin position="4"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="189" mass="21551" checksum="3037D3CF4F18E5AB" modified="2017-09-27" version="1">MRHLEFDGRYAEAILKGRKRATVRLGRRPNLKEGDVVLIHSGGYALGKAVIERVESKTVGELTDEDAFLDGFSSREELVNALKTHYKYVNDDSPAHVVVFRLTERFDKPVMSSDYAYEGNNPIEIAEMALKHLDLPEDDRKLIELFLRAGSLRKAAYRLGGMNKRYLIRGALRRAYEELKKRGLMGPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA09</accession>
    <name>A0A218PA09_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7344 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_03415</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_198362145.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA09"/>
    <dbReference type="GeneID" id="33315289"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03415"/>
    <dbReference type="OrthoDB" id="331021at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055768">
        <property type="entry name" value="DUF7344"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24035">
        <property type="entry name" value="DUF7344"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7344" evidence="2">
        <location>
            <begin position="2"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="155" mass="17975" checksum="29EB5B77D6091A25" modified="2017-09-27" version="1">MILGNERRMLLIEFLQMRNGRAELREIVEYIAEKEGNTDRKHRKSVYVSLMQTHIPKLEHEGVLTFERGVITLLQVPDNVTLYMEVVQRNDISWSTFYAGISFIFAITALWFGNSLLLFASLTYLLVAVVQHMKTYKVLRPRDEESPKKPDGNGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA17</accession>
    <name>A0A218PA17_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854872.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA17"/>
    <dbReference type="GeneID" id="33315180"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02870"/>
    <dbReference type="OrthoDB" id="85827at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="258" mass="29477" checksum="697768FAFA62712A" modified="2017-09-27" version="1">MVSVKGKIIFEIFIFPAVLFTALFGFTWAKLGVMTREWALITALLFVLVVGSMVFFLARILEKHGYRKSDIKRIDEILEEHWDEPWYSGYLKHDVQECIAHHLIIWGLLSTSLLAFHDVFFAIMALVGLVFLMVIMYPVFVTMVVWILALPLYYLKSRRAEDAFEFIAETSLVSTLAIPVIWAVSSYVSTKNYPEDVLKMFSAVVRNAEGFLLLSILNTLFGFLGGYLSRRVGRRVFAIVLLSLATAMLFIVWSIVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA38</accession>
    <name>A0A218PA38_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">3-hydroxy-3-methylglutaryl coenzyme A reductase</fullName>
            <shortName evidence="5">HMG-CoA reductase</shortName>
            <ecNumber evidence="5">1.1.1.34</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_05690</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>(R)-mevalonate + 2 NADP(+) + CoA = (3S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15989"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.34"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the HMG-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.34" evidence="5"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA38"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05690"/>
    <dbReference type="OrthoDB" id="10981at2157"/>
    <dbReference type="UniPathway" id="UPA00058">
        <property type="reaction ID" value="UER00103"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004420">
        <property type="term" value="F:hydroxymethylglutaryl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015936">
        <property type="term" value="P:coenzyme A metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00643">
        <property type="entry name" value="HMG-CoA_reductase_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.420:FF:000001">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.770.10">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl-coenzyme A Reductase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3270.10">
        <property type="entry name" value="HMGR, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.420">
        <property type="entry name" value="Hydroxymethylglutaryl-CoA reductase, class I/II, NAD/NADP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002202">
        <property type="entry name" value="HMG_CoA_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023074">
        <property type="entry name" value="HMG_CoA_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023076">
        <property type="entry name" value="HMG_CoA_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004554">
        <property type="entry name" value="HMG_CoA_Rdtase_eu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023282">
        <property type="entry name" value="HMG_CoA_Rdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009023">
        <property type="entry name" value="HMG_CoA_Rdtase_NAD(P)-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009029">
        <property type="entry name" value="HMG_CoA_Rdtase_sub-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00533">
        <property type="entry name" value="HMG_CoA_R_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572:SF24">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00368">
        <property type="entry name" value="HMG-CoA_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00071">
        <property type="entry name" value="HMGCOARDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55035">
        <property type="entry name" value="NAD-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56542">
        <property type="entry name" value="Substrate-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00066">
        <property type="entry name" value="HMG_COA_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00318">
        <property type="entry name" value="HMG_COA_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50065">
        <property type="entry name" value="HMG_COA_REDUCTASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0521">NADP</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="408" mass="43960" checksum="FF3A7C95AD9DB87E" modified="2017-09-27" version="1">MNFEELVEKVANGEIKLHQVEKYTNGDKKLATEIRRKALERKFGISLENIGHYSIDPNRVIGKNIENMIGVVQIPMGVAGPLKINGEYAKGEFYIPLATTEGALVASVNRGCSALTAAGGVKTTIIGDKMTRAPLLKCPDARRAREVAEWVKNNLDYLQEKAVSNVTRHGRLRDVKPYIVGNNLYLRFEFETGDAMGMNMVTISSEEIMKVIEEEFPDVKYLALSGNLCVDKKPNAMNFINGRGKTVIAEALIPREIVEKKLKTTPELIAEVNYRKNLVGSAQAGSYGFNAHFGNIVGAIFLATGQDEAQITEGSHGITLAEVTPEGDLYISVTMPSLEVGTVGGGTRVPTQREALSIMGVAGGGDPPGTNAKKFAEIIAGAVLAGELSLLAAIAAKHLAKAHKELGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA46</accession>
    <name>A0A218PA46_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NADH dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_06195</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA46"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06195"/>
    <dbReference type="OrthoDB" id="371891at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050586">
        <property type="entry name" value="CPA3_Na-H_Antiporter_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006419">
        <property type="entry name" value="PRK08668.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42703:SF1">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER SUBUNIT D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42703">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="305"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="374"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="416"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="450"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="502"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="598"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="7">
        <location>
            <begin position="116"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="617" mass="67050" checksum="29DFD612A8431884" modified="2017-09-27" version="1">MNELALIVFTPLLAGVLAWLLDIRGLREAIGVIGAAVPLAYAAMLYERAAEGLSFAINLGVFNITFALSQLNWFFVGIAALVGFSAILGLASTAKDSYEWLFALMSLTGVLGVFLASDLMTFFIFWEVMTFASFMMVLHYNKQASLKYFLLSVAGAYAMLIAIAFIYVKVGSFDFGAVQAALTQDAYAKMLGGQATFSSGELMALFGLFLVAFGVKAGLFPLHVWAPDAYSETNQSYTAMFSGVLSKAGVYGFILMYLLIGTRLAVEFGAFRSAPKFGYIIAFLGGLTIIVGGLLAALQEDIRKLFAYSSISQVGYILVGIGVGTALSMQAAMFHALSHALFKGLFFLIVATILHRTGKTTFADMGGLAEKMPFTFAMAFVAILSLAGIPPLVGFASKWVLFEAVISQNLPILGGMVFFGSAIGFVYLIRFTYAVWFGQRPTDLDDTKDAPLPLAVAMAILAFFNVVLGIAPGLVARELNKIFGSDVIGGDLFILDLGFGKYNALAILTYLIAGIAVAAVIYFLGAKVRKVPVTDTYQSGNPVTMDYNLTIRRNFFLPLKETLSGWLRISFDRTYRDIGAWVEDFGEVMRNYVYNGNVQSYAWYLLLILLILAMWGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA60</accession>
    <name>A0A218PA60_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ASCH domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07895</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854923.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA60"/>
    <dbReference type="GeneID" id="33316184"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07895"/>
    <dbReference type="OrthoDB" id="31314at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06552">
        <property type="entry name" value="ASCH_yqfb_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.30">
        <property type="entry name" value="Hypothetical protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007374">
        <property type="entry name" value="ASCH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42250">
        <property type="entry name" value="ASCH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42250:SF1">
        <property type="entry name" value="ASCH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04266">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01022">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ASCH" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="182" mass="20753" checksum="3E48CC046520345B" modified="2017-09-27" version="1">MLIDSAYKSRILRGDKVTTVRYGDYEAKPGSEVYLVVTPSDTAVAKVRITRVEKKKVKELTNEDAKLDGFSDVKELLGELSKIYGELYGDDEVTIIGFEVIKRFDDGIPLKWLKGLNYREPAEIARLYLENQEKLNLNRETDFIMRRIYNEGLGRAVRTFGPKKVQNALLKTYHALYAAGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA69</accession>
    <name>A0A218PA69_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">KaiC-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_08940</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA69"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08940"/>
    <dbReference type="OrthoDB" id="86164at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="209" mass="23195" checksum="7974A07CBC4CA413" modified="2017-09-27" version="1">MFFKPAELEPREVKPFDAFPLAFAEGSLSGIVSTDELAETVFLHHLIASSLENGPVYHVGPGSSLSLKSLRNLVEDLSNLYAGNVYSVDELLEALKIVDDGSLVIVSRFPALLNRSAEKLVEIRRLVDKKGITLVLGHSTMELNELDLPGEFRRLYDLPELFEALLVLRTSSYRGHYRMNLTVLRAPAEHVASVGDHSIPIDSLVRPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA79</accession>
    <name>A0A218PA79_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA79"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09395"/>
    <dbReference type="OrthoDB" id="85783at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="387" mass="44430" checksum="EB48AA63D1EF0624" modified="2017-09-27" version="1">MGPEGPEDFEQLAIKRINEGNLKEGLKLMLRAAKGYEDRRQKADAARLYKYLGYVLLEKTKSVEKSKPSLLKSAYLYLDLIDEEVSRPEVDIDALDKYCFNVLEIFATLDDHKNLMKYAEEFAAIYEDLGNSYQDNADIEIAIRAYESAYRYYRLIENMEAYRRIAEMMITLYGQVAEERLEKGNVKDAAEAFYRLAYFILAIFGYDIHFIEMMDTAAKNFEKASKVAYSNGDLDGTTSYLVKAQYSYLMARNFNRAKLIGLNTARMLYQVITSYRSNREEDKAASKLVELSEALIGIGKINEAMDAYRSALESKSNLAFRIRVRLAVLKGHAASKGSEVIVNEIDTIEYYLNRKNLMKALELAEKSMRREELMETLKKIHAAEGVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PAA5</accession>
    <name>A0A218PAA5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pullulanase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_07175</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PAA5"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07175"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11338">
        <property type="entry name" value="AmyAc_CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07184">
        <property type="entry name" value="E_set_Isoamylase_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.400.10">
        <property type="entry name" value="Oligo-1,6-glucosidase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357">
        <property type="entry name" value="ALPHA-AMYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357:SF210">
        <property type="entry name" value="MALTODEXTRIN GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="3">
        <location>
            <begin position="224"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="695" mass="78474" checksum="75110B353A5A3D0C" modified="2017-09-27" version="1">MYTGSEKGCASGRVPVTFTYDSGNKTVTSISLRGSFNDWDEWPMKEINGTWTITVCLAPGKYQYKYFINGQWVKDMSNDGTGRPYDPDADGYVDDGYGGKNAVRIVEGSAAFYVDFDPSDPAYLSIADNRTVIRFEAKRGTVSSATLVTDRGNYTMRLQVWWDSGEMWRAEVPLVEPVKYYILLSSADGERFAVLNTSENPFFSFDGVDRFPQLEWVSNGITYQIFPDRFNNGNRSNDALALDHDELLLNQVNPGTPVLSNWSDPITPLHCCHQYFGGDIKGITEKLDYLQSLGVTIIYLNPITLSGSAHGYDTYDYYRLDPKFGTEEELREFLDEAHKRGIRVIFDFVPNHCGIGNPAFLDVWKNGNQSPYWDWFFIKQWPFKLGDGNAYVGWWGIGSLPKLNTANPEVREYLIGAALHWLDFGFDGIRVDVPNEVLDPGVFFSELRKRVKEKHPEAYLVGEIWMLSPEWVKGDRFDSLMNYALGRDILLNYAKGLLSGETAMKMMGRYYASYGENVVAMGFNLVSSHDTSRVLTDLSGGSLGDTPTNESIQRLKLLSTLLYALPGTPVTFQGDERGLLGDKNHYDEQRYPIQWDEVNEDVLNHYRELAKLRRKTPALRSSAIRFYTADGGMMAFFRGHNDEVLVVANSWKSPAELELPPGEWKVVWPENYNGPTVVSDAIEVPPISVLVLESG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PAB6</accession>
    <name>A0A218PAB6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Adenylate cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854922.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PAB6"/>
    <dbReference type="GeneID" id="33316159"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07785"/>
    <dbReference type="OrthoDB" id="46040at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07890">
        <property type="entry name" value="CYTH-like_AC_IV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.320.10">
        <property type="entry name" value="Hypothetical Protein Pfu-838710-001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008173">
        <property type="entry name" value="Adenylyl_cyclase_CyaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033469">
        <property type="entry name" value="CYTH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023577">
        <property type="entry name" value="CYTH_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00318">
        <property type="entry name" value="cyaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21028:SF2">
        <property type="entry name" value="CYTH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21028">
        <property type="entry name" value="SI:CH211-156B7.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01928">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01118">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55154">
        <property type="entry name" value="CYTH-like phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51707">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CYTH" evidence="1">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="168" mass="19752" checksum="AD51B16A7E2FDC70" modified="2017-09-27" version="1">MEIEVKFRVDFNSLRAVIEALGASFVREEVQEDVYFSIPLPELLRVRRIPNLGKTFLTYKRIADPGRNEEFDELEVEVSDFETTVEILKRLGYKEDVIVRKRRLVYRLNRVTFELSEVEGLGGFLDIEVISDDVAEAKREIWEVARKLGLSEKDVEPRLYQELIRERT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P4Y2</accession>
    <name>A0A218P4Y2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Antitoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_00025</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Antitoxin component of a type II toxin-antitoxin (TA) system.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UPF0165 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853093.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P4Y2"/>
    <dbReference type="GeneID" id="33314608"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00025"/>
    <dbReference type="OrthoDB" id="116241at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1150.10">
        <property type="entry name" value="AF2212/PG0164-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008203">
        <property type="entry name" value="AF2212-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024069">
        <property type="entry name" value="AF2212-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01954">
        <property type="entry name" value="AF2212-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141694">
        <property type="entry name" value="AF2212/PG0164-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-1277">Toxin-antitoxin system</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU368051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ05831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="62" mass="7141" checksum="F7F2DCEFC3A6123B" modified="2017-09-27" version="1">MPEEIVAIYRGDVIIPLKKLNIPPGSRIKLLIEKVEVRDAMKELGYLKLLQEGEDAEELFEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P4Z3</accession>
    <name>A0A218P4Z3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853119.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P4Z3"/>
    <dbReference type="GeneID" id="33314635"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00155"/>
    <dbReference type="OrthoDB" id="27972at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11580">
        <property type="entry name" value="eIF2D_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21154">
        <property type="entry name" value="PUA_MJ1432-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.10:FF:000017">
        <property type="entry name" value="RNA-binding protein, containing PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.120">
        <property type="entry name" value="Pre-PUA domain, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022430">
        <property type="entry name" value="CHP03684"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015266">
        <property type="entry name" value="DUF1947"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016437">
        <property type="entry name" value="MCT-1/Tma20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03684">
        <property type="entry name" value="arCOG00985"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011151">
        <property type="entry name" value="PRK14560.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011153">
        <property type="entry name" value="PRK14560.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798:SF0">
        <property type="entry name" value="MALIGNANT T-CELL-AMPLIFIED SEQUENCE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798">
        <property type="entry name" value="MCT-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09183">
        <property type="entry name" value="DUF1947"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005067">
        <property type="entry name" value="Tma_RNA-bind_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUA" evidence="1">
        <location>
            <begin position="88"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="172" mass="19617" checksum="77EED443ABB239B9" modified="2017-09-27" version="1">MELKVKHPLSKKEVKEIIREMTEIFGEEIAKKMLNKKDRVELAEFDKTTEVILVNGRPFFIRRKELVFPLVIALYELSNEDDLRKWPRRVVVDAGAVPFIIKGADVMAAGITDADENIREGDFVFVVEEDYGRPLAIGIALMDGKTMKEKPKGKAVKNIHHAKDKIWELTVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P520</accession>
    <name>A0A218P520_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Biotin synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_00315</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853149.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P520"/>
    <dbReference type="GeneID" id="33314667"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00315"/>
    <dbReference type="OrthoDB" id="84887at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11570">
        <property type="entry name" value="Protein of unknown function DUF257"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005489">
        <property type="entry name" value="DUF257"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03192">
        <property type="entry name" value="DUF257"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ05886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="212" mass="23819" checksum="97705B930E172EF0" modified="2017-09-27" version="1">MDTSTLKKYLLGKANRGDSVLIEYESTYPVEGFSWGFLIPALLERNGVVVADFFGVGDLLFRNYIRRVSGREYSRTIELMRNIKVVKIGPGSASYGGVFEEITPTYEPSTFLKNYHTIISKMSRLPSKPEYVVTFGLGHYIHFGDDDAIRSLITAVSTIPTEDWVGVHFINEGVMRREHLAMLEGISSMVFHVSERGLKVKKEGESIDQGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P525</accession>
    <name>A0A218P525_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_00300</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853146.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P525"/>
    <dbReference type="GeneID" id="33314664"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00300"/>
    <dbReference type="OrthoDB" id="194307at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904680">
        <property type="term" value="F:peptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.105.10">
        <property type="entry name" value="Dipeptide-binding Protein, Domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290:SF9">
        <property type="entry name" value="OLIGOPEPTIDE-BINDING PROTEIN APPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PERIPLASMIC BINDING COMPONENT OF ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Solute-binding protein family 5" evidence="5">
        <location>
            <begin position="278"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="30"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="31"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ05883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="724" mass="81077" checksum="C32078A9B44EFC85" modified="2017-09-27" version="1">MKAKPTIALLVVFLVVFSVVASGCISGGGETTTISQSQTTTESQTGGQTTTSSGGTATSTQTTTQTPAEVKPGILEMDNVYVIVTDKSVVVVGPKGASPTVEVPTDRKVIKIEYEVDEANTPDVQTLMEQGQGFGAINPAFFRDEHVDALIIAARRETDPTIRTELFKALYMLGNKLVPEVILGQNRQLRVYWDWVKGRYYHPTLAERYDLLSEDSSAPTVKIGIKDYTNDPETYTIATIGWPESFDPAMTYETFGWEIWHEIGDTLVTYWKEDTETVSPDLAVAWAHNKDGTEWYFLIRGGVKAYDPWNDKTYPIDATDVAFTFLRVERLGHSVSWMVDGFMDVNNSAALTEDEFDQYLKEHPLVAEFGGKSTEVKSLDELKQFFGYSGDTAGVFKLVLPQPYAPVLGILADPFLSVVPMEYLLGDKYEEALAASDNGHNPSAWWNYLSEGKSDPTHQLMHNNPVGTGPFYVADYQKDAYIVLEYNPYYWNATATPGHKRVVYIINSDAMARINIFKTGTADVVVIPPEKMDTVKGLTLDGFNSVVQTDILQPILTFLVFNTQKEPFNDPLVREALAYAVPYDQIKQIVYQGLLEMNYGPIPKPWPGYTEEGIIKYNYNLAKAKQLLNQAGVDPTQYKIELIYNEGNAAREKIMTLLQNVWSQLGFQVTVNSYNWPTYLDKTEHGEYDVYIVGWVPDYLDSDNWVGPFLYGATEFKSLEITVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P567</accession>
    <name>A0A218P567_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853181.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P567"/>
    <dbReference type="GeneID" id="33314702"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00490"/>
    <dbReference type="OrthoDB" id="146310at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002825">
        <property type="entry name" value="Pept_S49_ser-pept_pro"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047768">
        <property type="entry name" value="Clp_like_SDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35984">
        <property type="entry name" value="PERIPLASMIC SERINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35984:SF4">
        <property type="entry name" value="PERIPLASMIC SERINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01972">
        <property type="entry name" value="SDH_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="31621" checksum="887450C8F5D43BA5" modified="2017-09-27" version="1">MDPLSGFFGSLLWWLFFLYLLMWPQLQFRALQAARARIMAQLARKRNSTVIAMIHRQESVGLFGIPVYKFISIEDSEEILRAIRTAPKDKPIDLIVHTPGGLVLAATQIAKALKEHPAETRVIVPHYAMSGGTLIALAADKIIMDPNAVLGPVDPQLGQYPAPSILRAVEKKGPEKVDDQTLILADVAEKAIKQVRDFVYNLLKDKYGEEKAKELAQILTEGRWTHDYPITVDHARELGLHVETDVPEEVYALMELYKQPVKQRGTVEFMPYPVRQESKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P580</accession>
    <name>A0A218P580_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_00615</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853204.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P580"/>
    <dbReference type="GeneID" id="33314727"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00615"/>
    <dbReference type="OrthoDB" id="372125at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01896">
        <property type="entry name" value="DRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01666">
        <property type="entry name" value="TGS_DRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045001">
        <property type="entry name" value="DRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031662">
        <property type="entry name" value="GTP-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43127">
        <property type="entry name" value="DEVELOPMENTALLY-REGULATED GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16897">
        <property type="entry name" value="MMR_HSR1_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="4">
        <location>
            <begin position="83"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="5">
        <location>
            <begin position="313"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="39"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ05942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="388" mass="43212" checksum="C6475F175B7C5036" modified="2017-09-27" version="1">MPTNVTAEYLAAEEEYRNAKTIPEKIRALEKMYATVPKHKGTEKLRLQIKRKLAELRKELDKQRQMKKGGGGPSLAVRKEGAAQIVLAGLPNVGKSSLMKALTNVDADVADYAFTTVEPIPGMMHHKDVQIQLVEVPGLVEGAALGKGMGPQLLSVIRNADAIAIVVDLSRDPVKQMEILLKEFERAGIKVNKRKPRVEIKKTAMGGIVINGQENIKGDIQEVMKMLREERIHSAEITVKEPVTLEEIADALDESLVWRRAIIIANKGDAPGSKENYERLLKAYGDRFKIIPVSAKRKIQLDKLKDELYELAGIIRVFTKSPGEEPAYPPVPLKKGSTVMDLAERIHKDFAKNFRYARVWGKSVKFPGQRVGADHVLEDGDIVEIHAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P594</accession>
    <name>A0A218P594_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dihydroorotate dehydrogenase</fullName>
            <shortName evidence="10">DHOD</shortName>
            <shortName evidence="10">DHODase</shortName>
            <shortName evidence="10">DHOdehase</shortName>
            <ecNumber evidence="10">1.3.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pyrD</name>
        <name evidence="12" type="ORF">A3L08_00695</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the conversion of dihydroorotate to orotate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(S)-dihydroorotate + A = orotate + AH2</text>
            <dbReference type="Rhea" id="RHEA:18073"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="10">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Pyrimidine metabolism; UMP biosynthesis via de novo pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterotetramer of 2 PyrK and 2 PyrD type B subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.3.-.-" evidence="10"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853218.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P594"/>
    <dbReference type="GeneID" id="33314743"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00695"/>
    <dbReference type="OrthoDB" id="36608at2157"/>
    <dbReference type="UniPathway" id="UPA00070"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004152">
        <property type="term" value="F:dihydroorotate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04740">
        <property type="entry name" value="DHOD_1B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000027">
        <property type="entry name" value="Dihydropyrimidine dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00224">
        <property type="entry name" value="DHO_dh_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050074">
        <property type="entry name" value="DHO_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033888">
        <property type="entry name" value="DHOD_1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024920">
        <property type="entry name" value="Dihydroorotate_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012135">
        <property type="entry name" value="Dihydroorotate_DH_1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049622">
        <property type="entry name" value="Dihydroorotate_DH_I"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005574">
        <property type="entry name" value="PRK07259.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01037">
        <property type="entry name" value="pyrD_sub1_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109:SF1">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (FUMARATE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (QUINONE), MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000164">
        <property type="entry name" value="DHO_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00911">
        <property type="entry name" value="DHODEHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00912">
        <property type="entry name" value="DHODEHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 10" id="KW-0288">FMN</keyword>
    <keyword evidence="9 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 10" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydroorotate dehydrogenase catalytic" evidence="11">
        <location>
            <begin position="4"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="45"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="69"/>
            <end position="73"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="188"/>
            <end position="189"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="239"/>
            <end position="240"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="261"/>
            <end position="262"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ05956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="301" mass="32354" checksum="DF00E4166E9844D6" modified="2017-09-27" version="1">MASLEVELFGIRFENPLILASGINDKVPEQWIRAHEEGAGGVVTKSIGIEPRKGYDNPTIVELPYGLINAMGLPNPGWRGFLEMVDGYTFDFPLIVSIFGGTPEEFAFLAEKLSEVADAFELNLSCPHAKGYGMEIGQKTENVYEVVKAVKDATDKPVIAKLTPNTDDITKLGLAAEKAGADAVSAINTLKAIAIDIYARRPILSNRVGGYSGPGVKPVALRAVYDLARTLDIPVIGIGGITTWQDAVEFLLAGASALQIGTALSLRGWKVFREISEGIERYLEGEGFSSVEEIVGLALEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5D2</accession>
    <name>A0A218P5D2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Lysine--tRNA ligase</fullName>
            <ecNumber evidence="10">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Lysyl-tRNA synthetase</fullName>
            <shortName evidence="10">LysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">lysS</name>
        <name evidence="12" type="ORF">A3L08_00890</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>tRNA(Lys) + L-lysine + ATP = L-lysyl-tRNA(Lys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="10"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853251.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5D2"/>
    <dbReference type="GeneID" id="33314782"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00890"/>
    <dbReference type="OrthoDB" id="6838at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00674">
        <property type="entry name" value="LysRS_core_class_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.10">
        <property type="entry name" value="Lysine tRNA ligase, stem contact fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00177">
        <property type="entry name" value="Lys_tRNA_synth_class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002904">
        <property type="entry name" value="Lys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042078">
        <property type="entry name" value="Lys-tRNA-ligase_SC_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00467">
        <property type="entry name" value="lysS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37940">
        <property type="entry name" value="LYSINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37940:SF1">
        <property type="entry name" value="LYSINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01921">
        <property type="entry name" value="tRNA-synt_1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="An anticodon-binding domain of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class I anticodon-binding" evidence="11">
        <location>
            <begin position="445"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="10">
        <location>
            <begin position="30"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="10">
        <location>
            <begin position="280"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF19269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ05989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="526" mass="61793" checksum="31DABB12D88FA742" modified="2017-09-27" version="1">MVHWADYMAERIIRERGDKEEYVVESGITPSGYVHIGNFREFFTAYIVGHALRDRGKKVRHIHMWDDYDRFRKVPKNVPAEWKEHLTKPVREVPDPWGCHDSYAEHFMALFEEEISRLGIEADFLHAYELYKSGEYAEEVKLALQKRDEIKAILDKYRERAKQPPLEDSWQPVMVYCPHCRKEADFVSWDGEWKVSYRCPHCGREGETDIREGNVKLRWRVDWPMRWAHFKVDFEPAGKDHLAAGGSYDTGREIVEKVFGWKAPIDLMYEFVGIKGQKGKMSGSKGNVILLSDLYEVLEPGIIRFIYAKARPNKELKIDLGLGLLNLYDEFDKVERIYFGLEKAKNSEEEEELKRTYELSMPKVPEKLTAQAPFRFLVTLVQMPHLDEEGIIRILQEQGHVPEELSKEDIERIKLRIRLAKNWVEKYAPEDVKFSLLERPPEIELEPEIREAMLEVAEWLEKNERFTVDELNNVIFDAAKKRGIPSKKWFKALYNVFIGKDRGPRLAPFLASLKREFVIGRLRMEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5E3</accession>
    <name>A0A218P5E3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Extradiol dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853276.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5E3"/>
    <dbReference type="GeneID" id="33314810"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01030"/>
    <dbReference type="OrthoDB" id="55814at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07952">
        <property type="entry name" value="ED_3B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.830.10">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004183">
        <property type="entry name" value="Xdiol_dOase_suB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02900">
        <property type="entry name" value="LigB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53213">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Extradiol ring-cleavage dioxygenase class III enzyme subunit B" evidence="1">
        <location>
            <begin position="6"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="261" mass="29728" checksum="961F04D8956172EF" modified="2017-09-27" version="1">MLIGVAIMPHGNPVLEPEDDETRRLTEVLRKIGEEFKEADSYVLISPHNVRMSDHLGIVMAEHLISWLGFDGVELPGEWETDRELAEGIYEATKDRFPVVDLHFASLRGEYSRWPLSWGELIPLQFLEKKPLVLLTPARSLSRETLIEFGEALGEVMEKSKKRIALIVSADHGHAHDENGPYGYRKESEEYDRLIMRLINENRLEELPKISEELVNKALVDSYWQMLIMLGAMGVGNFELKESAYACPTYFGMAGALWVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5I0</accession>
    <name>A0A218P5I0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BtpA-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BtpA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853297.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5I0"/>
    <dbReference type="GeneID" id="33314834"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01150"/>
    <dbReference type="OrthoDB" id="38543at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd04722">
        <property type="entry name" value="TIM_phosphate_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005137">
        <property type="entry name" value="BtpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00259">
        <property type="entry name" value="thylakoid_BtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21381:SF3">
        <property type="entry name" value="SGC REGION PROTEIN SGCQ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21381">
        <property type="entry name" value="ZGC:162297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03437">
        <property type="entry name" value="BtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005956">
        <property type="entry name" value="BtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="28293" checksum="C1D7C8A713A2A1BC" modified="2017-09-27" version="1">MDFERKPLIGMVHLKPLPGSYLYDGDLDGVIEAALRDAKTIEKAGFDAVMVENFGDVPFPKYVDKTTVAAFTAVVKEIRDEISLPLGINVLRNDGIAAYSIAYAVKADFIRVNVLSGVAYTDQGIVEGIAHELAKLRKLLPSKIRVFADVHVKHAVHFGDFEDSLMDTVERGLADAVVVSGKATGKPVDLEKLALAKRISPVPVVVGSGTTYDNLPELWEYADGFIVGTWIKRDGKVENEVSLERARKLVELAKELRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5K6</accession>
    <name>A0A218P5K6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="11">2-ketoisovalerate ferredoxin oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">vorD</name>
        <name evidence="11" type="ORF">A3L08_01310</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterotetramer of one alpha, one beta, one delta and one gamma chain.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853326.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5K6"/>
    <dbReference type="GeneID" id="33314867"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01310"/>
    <dbReference type="OrthoDB" id="23478at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011898">
        <property type="entry name" value="PorD_KorD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02179">
        <property type="entry name" value="PorD_KorD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007202">
        <property type="entry name" value="PRK09623.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43724">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43724:SF1">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14697">
        <property type="entry name" value="Fer4_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="10">
        <location>
            <begin position="44"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="10">
        <location>
            <begin position="74"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="105" mass="11867" checksum="463ABD0606B41ACF" modified="2017-09-27" version="1">MNTLFGERKEGATKIVLTKVDEYPEAPISLGTTLSNFTGDWRTFIPIVDESKCVKCYICWKFCPEPAIYIKEDGYVAVDYDYCKGCGICANECPTKAITMEKEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5M9</accession>
    <name>A0A218P5M9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Metal-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01435</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853348.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5M9"/>
    <dbReference type="GeneID" id="33314892"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01435"/>
    <dbReference type="OrthoDB" id="94803at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018664">
        <property type="entry name" value="DUF2103_metal-binding"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09876">
        <property type="entry name" value="DUF2103"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="97" mass="10979" checksum="01CC7889FD6F0EDB" modified="2017-09-27" version="1">MPKHFHKGVKREHHFLKGLEKPLEEIARIPGVKKVIPGRIYASDSRGFEIKVTRETQTGLKLVAKSDGSVQEVFLVVDKADRVRVWGEIEKLFGGDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5S5</accession>
    <name>A0A218P5S5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01835</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853419.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5S5"/>
    <dbReference type="GeneID" id="33314972"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01835"/>
    <dbReference type="OrthoDB" id="91968at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="283" mass="32170" checksum="CA7B2C54872F5B3C" modified="2017-09-27" version="1">MDPGAVIFLLFIAAIVGMILMGIKGEESSRERCRRFSESVAVEGTFLKLPIETKLSKGKFVLRGEWRGSKNRYYHIERSFEALEELSAGEIELTPEPFSVEVKKNDDALVELLAYIVEEGEFKNAVIVPIIPSYSVEIEKDTLEASKDMEFAHLRVEVIESGLYGKLYVNVSKCRGARVEFEKNGIHTREKLVEVKESGEAEFRREFWTKPLILIMDRNHADPRKLRDVFGKQEILEGHGEYLIRLTLDVPFSKDEHDTSRVTVSPGGEVPAELKEPEVKVVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5V3</accession>
    <name>A0A218P5V3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Adenylyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_01930</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HesA/MoeB/ThiF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853437.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5V3"/>
    <dbReference type="GeneID" id="33314991"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01930"/>
    <dbReference type="OrthoDB" id="7915at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00757">
        <property type="entry name" value="ThiF_MoeB_HesA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000080">
        <property type="entry name" value="Thiazole biosynthesis adenylyltransferase ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006204">
        <property type="entry name" value="PRK08328.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF102">
        <property type="entry name" value="ADENYLYLTRANSFERASE AND SULFURTRANSFERASE MOCS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="2">
        <location>
            <begin position="10"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="232" mass="25734" checksum="295EA80B24010ACA" modified="2017-09-27" version="1">MLSEKELERYDRQIMILGKEGQEKLKNSKVAVVGVGGLGSPVAYYLAAAGIGTILLIDEQTPELSNLNRQILHWEEDLEKNPKPLSAKWKLERFNSEVRIETFVGRLDESNIDDVLAGVDIIVDCLDNFETRFLLDDYSQRKRVPLVHGAVEGTFGQVTTVVPGITKSLREIFPNIKEKSGKFPILGATAGVIGSIQTMEVIKLLTGIGEPLLNKLLLVDLAFNTFDVVDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5V5</accession>
    <name>A0A218P5V5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eight-cysteine-cluster domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853425.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5V5"/>
    <dbReference type="GeneID" id="33314979"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01870"/>
    <dbReference type="OrthoDB" id="31553at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027552">
        <property type="entry name" value="CGP_CTERM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027553">
        <property type="entry name" value="Heavy_Cys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027556">
        <property type="entry name" value="Heavy_Cys_CGP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04288">
        <property type="entry name" value="CGP_CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04289">
        <property type="entry name" value="heavy_Cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04292">
        <property type="entry name" value="heavy_Cys_CGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="365"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="405" mass="44132" checksum="9731D6DE1507582D" modified="2017-09-27" version="1">MKRLAALIVFLVFTLTPFAEACMSPADAYAVEVVLNKPGVVYRPYPAFNALHNAVIENGTFVFRSHYDRRLYVVLWNASDGPHIRIQIPVKWESETALSAHLNVSLFLLNEGIEKLKRTGWKVRDNTTFEKGGIRITLSPVKGSECTSDADCATGGCSGEMCAPREKASGIMTPCVYRPWYTCLAMTSCGCVNGVCTWKPNPAFESCLKEHGVDPSSVIKAGRFELSVEGANVSDEEVKASVEEFLGAFGVSCSNSFNLTKTSVTKPVPVVEPSKVNASEAMKTELEWLISVGALKIDEEDIEEIARVSEWGNAGWNSHVGWYETKNGTYSWIPYDKSLNPKLVKCFTNVIPEYKLPNGTAYVGPTMTKPSPSSSSATSGDSSGESICGPALIVGLSLVALLWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5V8</accession>
    <name>A0A218P5V8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">dITP/XTP pyrophosphatase</fullName>
            <ecNumber evidence="10">3.6.1.66</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Non-canonical purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Non-standard purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Nucleoside-triphosphate diphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Nucleoside-triphosphate pyrophosphatase</fullName>
            <shortName evidence="10">NTPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">A3L08_01900</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ITP + H2O = IMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>XTP + H2O = XMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28610"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>dITP + H2O = dIMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28342"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61194"/>
            <dbReference type="ChEBI" id="CHEBI:61382"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10 11">Belongs to the HAM1 NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.66" evidence="10"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853431.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5V8"/>
    <dbReference type="GeneID" id="33314985"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01900"/>
    <dbReference type="OrthoDB" id="372108at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035870">
        <property type="term" value="F:dITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036220">
        <property type="term" value="F:ITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036222">
        <property type="term" value="F:XTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009146">
        <property type="term" value="P:purine nucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00515">
        <property type="entry name" value="HAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.950.10:FF:000001">
        <property type="entry name" value="dITP/XTP pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01405">
        <property type="entry name" value="Non_canon_purine_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020922">
        <property type="entry name" value="dITP/XTP_pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002637">
        <property type="entry name" value="RdgB/HAM1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011396">
        <property type="entry name" value="PRK14821.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00042">
        <property type="entry name" value="RdgB/HAM1 family non-canonical purine NTP pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067:SF9">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE/HAM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01725">
        <property type="entry name" value="Ham1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="7"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="139"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="167"/>
            <end position="168"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="184" mass="20882" checksum="26988BEFCB20F77D" modified="2017-09-27" version="1">MRIAFITSNPGKVEEAKKYFEPLGVEVYQLHFPYPEIQADTLEEVAEYGAKWLAKRIDGPFFLDDSGLFINALNGFPGVYSAYVYKTLGIDGILKLMEGVEDRSAYFKSVVAYWDGEVHLFTGRVDGEITRGKRGSKGFGFDPVFKPSGFERTFAEMTTKEKNRISHRGRALKAFAEWLKENLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Y0</accession>
    <name>A0A218P5Y0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">acetate--CoA ligase (ADP-forming)</fullName>
            <ecNumber evidence="2">6.2.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_01985</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP + CoA = acetyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15081"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="6.2.1.13" evidence="2"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853448.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5Y0"/>
    <dbReference type="GeneID" id="33315002"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01985"/>
    <dbReference type="OrthoDB" id="18103at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043758">
        <property type="term" value="F:acetate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051538">
        <property type="entry name" value="Acyl-CoA_Synth/Transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043938">
        <property type="entry name" value="Ligase_CoA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032875">
        <property type="entry name" value="Succ_CoA_lig_flav_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43334">
        <property type="entry name" value="ACETATE--COA LIGASE [ADP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43334:SF2">
        <property type="entry name" value="ACETATE--COA LIGASE [ADP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19045">
        <property type="entry name" value="Ligase_CoA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13607">
        <property type="entry name" value="Succ_CoA_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA-binding" evidence="6">
        <location>
            <begin position="6"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="440" mass="47317" checksum="26F0AB30F90A5915" modified="2017-09-27" version="1">MSLDSFFYPESVAVFGSFKEGAIAYEILRNIVEGGFEGWIIPVNPKGGTVEVAGRTFEIRQKLEEPVDVAIIAVPAKIVPALIDEIGPLIKGAVVISAGFSEVGNVELERELVEKAKKHGIRIIGPNCAGIFGVHGKFFGSFEVRVKPGGLALISQSGAFGGAALAMGNDEGIGFSAFVSYGNAADLNESDFLEYFADDENTKAIALYIEGVKDGRRFLKALRYAANKKPVIVLKAGKSASGAKAAASHTGSLAGSYEIYRAAFKQAGAIEVEEMEELFDAAKAFEMYPKAGKRVAVITNSGGPGVLATDKLEKLGLEIAKLSEETVQKLRSFLPPQCSVKNPIDLIADADYERYKRTVETVCRDENVDSLLVICVPPIFIPSEEIAKAVIEADCNKPVIVNFMAGELVRDGVGLLERHGVKNFPTPERAARALKWLSQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P676</accession>
    <name>A0A218P676_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UPF0273 protein A3L08_02540</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_02540</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPF0273 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853548.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P676"/>
    <dbReference type="GeneID" id="33315114"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02540"/>
    <dbReference type="OrthoDB" id="27015at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19486">
        <property type="entry name" value="KaiC_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01076">
        <property type="entry name" value="UPF0273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010624">
        <property type="entry name" value="KaiC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022475">
        <property type="entry name" value="UPF0273_KaiC-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03877">
        <property type="entry name" value="thermo_KaiC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637:SF1">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01874">
        <property type="entry name" value="DNAREPAIRADA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51146">
        <property type="entry name" value="KAIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KaiC" evidence="4">
        <location>
            <begin position="3"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="30"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="248" mass="27577" checksum="D895507764FBCE0B" modified="2017-09-27" version="1">MIKRVKTGIPGMDEILHGGIPERNVVLLSGGPGTGKSIFSQQFLWNGLQMGEPGIYVALEEHPVQVRQNMAQFGWDVRKYEEEGLFAMVDAFTAGIGKSKEYEKYIVHDLTDIREFIDVLRTAVKDIGAKRLVVDSVTTLYINKPAMARSIVMQLKRVLAGLGVTSIFVSQISVGERGFGGPGVEHGVDGIIRLDLDEIDGELKRSLIVWKMRGTSHSMRRHPFEITDRGIIVHHDKVLKRGGIVETE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6G9</accession>
    <name>A0A218P6G9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">dTDP-glucose 4,6-dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_03040</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853640.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6G9"/>
    <dbReference type="GeneID" id="33315214"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03040"/>
    <dbReference type="OrthoDB" id="4907at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008460">
        <property type="term" value="F:dTDP-glucose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009225">
        <property type="term" value="P:nucleotide-sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05246">
        <property type="entry name" value="dTDP_GD_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000304">
        <property type="entry name" value="UDP-glucose 4,6-dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005888">
        <property type="entry name" value="dTDP_Gluc_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01181">
        <property type="entry name" value="dTDP_gluc_dehyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="4">
        <location>
            <begin position="4"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="333" mass="38353" checksum="1B0CDB16385CAF7F" modified="2017-09-27" version="1">MRLLVTGGMGFIGSNFIHYVLEKHPDWEVINLDKLGYGSNPANLKDIEDDPRYTFVRGDIADFELVSELIKKADAVVNFAAESHVDRSISSPEHFLRSNVIGTYTILEAIRKENPEVRLVHVSTDEVYGDILDGSFTERDALMPSSPYSATKAASDVLVLGWTRTYNLNASITRCTNNYGPYQFPEKLIPKTIIRASMGLKIPVYGTGQNVRDWLYVEDHVRAIEAVLLKGEPREIYNISAGEERTNLEVVKTVLKLMGKDEDLIEFVEDRPGHDLRYSLDSWKITRDLKWRPRHSFEEGIEKTVKWYLENEWWWRPLVNEKVLHPTPWKLGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6H6</accession>
    <name>A0A218P6H6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_02935</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853622.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6H6"/>
    <dbReference type="GeneID" id="33315193"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02935"/>
    <dbReference type="OrthoDB" id="135313at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="294" mass="33029" checksum="609697FE97F2313E" modified="2017-09-27" version="1">MKWKPLFAVLLGLLMIGMAAGSAAAMPSPSQNSHTKAVVMKEFPTKLLVDTPTRQTFVFGDLLVDYRTNGIEAKIIITNTTTGEIIDVVHLTSTKINDSYILTMEDTSGTTYSIRTPINMIDPGLKTKQLISKPGINKESSNIMTLTTQQHYWWDGVYFVKGYMIRYPHPDYEYYGIEPWDTASIKGNKLTHLHFSWAKSKFLLDAGPTALGAAIGAYFANVPGAVIGAITGLAAGITFGNYLLDEHGCLWVWFAWKWEYHWRWIPPGYEYGPVLKYLRIASYTLKNDYHLSNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6I5</accession>
    <name>A0A218P6I5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_03120</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853654.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6I5"/>
    <dbReference type="GeneID" id="33315230"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03120"/>
    <dbReference type="OrthoDB" id="11098at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04179">
        <property type="entry name" value="DPM_DPG-synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050256">
        <property type="entry name" value="Glycosyltransferase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48090:SF7">
        <property type="entry name" value="RFBJ PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48090">
        <property type="entry name" value="UNDECAPRENYL-PHOSPHATE 4-DEOXY-4-FORMAMIDO-L-ARABINOSE TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="2">
        <location>
            <begin position="5"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="294" mass="32372" checksum="77BBC14C81AF2A73" modified="2017-09-27" version="1">MRTLIIIPAYNEELTIGSVVALARKHGDVLVVDDGSKDRTSEIARESGAVVIRHEVNGGKGKALGTGFEYAFSNGYDFVVTIDADGQHNPDEIPVLLEPLLKGEADLVIGSRFLNGAKKNIPLYRRLGLWVLNVTTNMNLGDGLKITDSQSGFRAMNRKVLGEILQINSEGYKVESDMLVHLSERGFRIREVPINVRYDVPNKHKKNPVRHGFEVLAGLIGLIEYKRPLLLFGVSSLVSFIIAAGLAYWGLSPYYRGEGVYLTQLIGAGIFVIIGIQLFVAGLTLNVLRKLVRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6J1</accession>
    <name>A0A218P6J1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9 10">Quinolinate synthase</fullName>
            <ecNumber evidence="2 9 10">2.5.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nadA</name>
        <name evidence="11" type="ORF">A3L08_03205</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>iminosuccinate + dihydroxyacetone phosphate = quinolinate + phosphate + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25888"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
            <dbReference type="EC" id="2.5.1.72"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from iminoaspartate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the quinolinate synthase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.72" evidence="2 9 10"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853666.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6J1"/>
    <dbReference type="GeneID" id="33315247"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03205"/>
    <dbReference type="OrthoDB" id="5931at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00327"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008987">
        <property type="term" value="F:quinolinate synthetase A activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034628">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-aspartate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10800">
        <property type="entry name" value="NadA-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00568">
        <property type="entry name" value="NadA_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003473">
        <property type="entry name" value="NadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036094">
        <property type="entry name" value="NadA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023066">
        <property type="entry name" value="Quinolinate_synth_type2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00550">
        <property type="entry name" value="nadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006878">
        <property type="entry name" value="PRK09375.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30573:SF0">
        <property type="entry name" value="QUINOLINATE SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30573">
        <property type="entry name" value="QUINOLINATE SYNTHETASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02445">
        <property type="entry name" value="NadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142754">
        <property type="entry name" value="NadA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0408">Iron</keyword>
    <keyword evidence="8 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="112"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="199"/>
            <end position="201"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="NCBIfam" id="TIGR00550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="302" mass="34104" checksum="6D5528281D5653DA" modified="2017-09-27" version="1">MDVDGLIDEIIRLKEERNAIIMAHNYQLPEIQDIADFLGDSLELARKAVNVDAEVIVFAGVDFMAETAKILNPEKTVLLPTRRATCAMANMLKPEHIIEAKKRYPDAPVVLYVNSSAECKALADVTVTSANAAKIVGKLDSDVVIFGPDNNLAHYVAKRTGKTIIPIPEGGHCYVHRRFTLEDVERARKLYPNAKLMVHPECNPEVQEAADLIVSTGGMIRRAPEWNEWVVFTEREMVYRLSKLYPDIKFHPAREDATCIGMKAITLNHIYESLRDMKYEVEVPKEIAEKARKAIERMLEMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6J8</accession>
    <name>A0A218P6J8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853633.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6J8"/>
    <dbReference type="GeneID" id="33315206"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03000"/>
    <dbReference type="OrthoDB" id="102281at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="184" mass="20364" checksum="F9401195B22D9A43" modified="2017-09-27" version="1">MIGLMAALLAGVVLKQWAFGLAVVPYLLRLRSRNLSLIAFYAYVLTVVLMVPGVSIYTHEGLVQAVGAFTSTFLLLDEVLRGVKISRTELALSALLLASAVYDYAFVAALIAVTIYAVYLRFGRVVYYILGWLVTSAVVLYLLKNSLPDRVAQSFVMIGLGLIFLLFAERRDVEFLEVGLFEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6K4</accession>
    <name>A0A218P6K4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribonuclease VapC</fullName>
            <shortName evidence="5">RNase VapC</shortName>
            <ecNumber evidence="5">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Putative toxin VapC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">vapC</name>
        <name evidence="7" type="ORF">A3L08_03055</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Toxic component of a toxin-antitoxin (TA) system. An RNase.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PINc/VapC protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="5"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853643.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6K4"/>
    <dbReference type="GeneID" id="33315217"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03055"/>
    <dbReference type="OrthoDB" id="93300at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18677">
        <property type="entry name" value="PIN_MjVapC2-VapC6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00265">
        <property type="entry name" value="VapC_Nob1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022907">
        <property type="entry name" value="VapC_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39677">
        <property type="entry name" value="RIBONUCLEASE VAPC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39677:SF4">
        <property type="entry name" value="RIBONUCLEASE VAPC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0800">Toxin</keyword>
    <keyword evidence="1 5" id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="domain" description="PIN" evidence="6">
        <location>
            <begin position="4"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="156" mass="17532" checksum="9B9672705A18DDB5" modified="2017-09-27" version="1">MGPLTAFVDTNVIIEHLEGNVDLLDIRERFDALYSNNIVFSEALMVYLMALTGERPYTLKHNPNIIRNLSEELLDFLGLFGLFLDLEINRAVETLAVEYMIKYGLLPNDALILATCKFHGLKYLISFDSDFANACESEGISLISDPEEITRVGDVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6L3</accession>
    <name>A0A218P6L3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Phosphoribosylformylglycinamidine cyclo-ligase</fullName>
            <ecNumber evidence="4 15">6.3.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 15">AIR synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13 15">AIRS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11 15">Phosphoribosyl-aminoimidazole synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">purM</name>
        <name evidence="18" type="ORF">A3L08_03240</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ATP = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the AIR synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.1" evidence="4 15"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853672.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6L3"/>
    <dbReference type="GeneID" id="33315254"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03240"/>
    <dbReference type="OrthoDB" id="6605at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00129"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004641">
        <property type="term" value="F:phosphoribosylformylglycinamidine cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046084">
        <property type="term" value="P:adenine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02196">
        <property type="entry name" value="PurM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.10:FF:000020">
        <property type="entry name" value="Phosphoribosylformylglycinamidine cyclo-ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.650.10:FF:000011">
        <property type="entry name" value="Phosphoribosylformylglycinamidine cyclo-ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00741">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004733">
        <property type="entry name" value="PurM_cligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00878">
        <property type="entry name" value="purM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520:SF12">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 15 18" id="KW-0436">Ligase</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 15" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="16">
        <location>
            <begin position="45"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="17">
        <location>
            <begin position="171"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ASJ06414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="334" mass="36271" checksum="421E5969271837C5" modified="2017-09-27" version="1">MLTYAQAGVDDEKTANALKGIISLAKATFEFRKGRIGEPAEDLGHYAALMDFGNFYLAMTTDGVGTKVLVAEAVGKFDTIGIDMIAMNVNDLLCVGAEPVALVDYLAVREPDEKVFSEIAKGLYEGARQAGIAIVGGETAVMPDLINGFDLAGTAIGVVEKGKVITGERIRPGDAVIGISSSGIHSNGLTLARKLLIPKYGLDYEYEGKKLWEWLLEPTRIYVKPVLELLESVEVHGLAHITGGGLLNLKRLTNYGFSLEMPPIAGIFRLIHESGVPLDEMFRVFNMGVGMVAIVPPEEKEEALEVLNKHYESFELGTVTREPGIRVENYRVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6N9</accession>
    <name>A0A218P6N9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">LSU ribosomal protein L41E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03460</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6N9"/>
    <dbReference type="SMR" id="A0A218P6N9"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03460"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="37" mass="5029" checksum="3F28958580286FB9" modified="2017-09-27" version="1">MKRRPRKWKKKGRMRWKWIKKRIRRLKRQRRKERGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6S5</accession>
    <name>A0A218P6S5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Replication protein RepA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_03615</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853737.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6S5"/>
    <dbReference type="GeneID" id="33315328"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03615"/>
    <dbReference type="OrthoDB" id="85479at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03524">
        <property type="entry name" value="RPA2_OBF_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040260">
        <property type="entry name" value="RFA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13989">
        <property type="entry name" value="REPLICATION PROTEIN A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13989:SF16">
        <property type="entry name" value="REPLICATION PROTEIN A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OB" evidence="2">
        <location>
            <begin position="43"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="271" mass="31363" checksum="11218C9ACD97309F" modified="2017-09-27" version="1">MKKRLPASRVYIMDIINGYFVKSEGDFEPNYLITRDARKVYRVKVVATVVREPAISEDETYGKFQIDDGTGTIWVLGFREDTRFIRLVKKGDLVQIIGKISEWRDDKQILVEGISKVEPNMWILHRFETLKEKVEHAKKAKIAFEIYDKYGVTAKAKVIAKNKGVSEDMLLTIDELYTMMLEQRSAEEALFEEEISEEISEEEEAPTNPELEKAKNAVLELLKEKEKALSHKFIVKKLSKDFSEELIEEAITQLLAEGEIYEPEIGYYEPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6T4</accession>
    <name>A0A218P6T4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Iron-dependent repressor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_03650</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DtxR/MntR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853741.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6T4"/>
    <dbReference type="GeneID" id="33315335"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03650"/>
    <dbReference type="OrthoDB" id="24735at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000189">
        <property type="entry name" value="HTH-type transcriptional regulator MntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.60.10:FF:000005">
        <property type="entry name" value="Transcriptional regulator MntR protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.10">
        <property type="entry name" value="Iron dependent repressor, metal binding and dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050536">
        <property type="entry name" value="DtxR_MntR_Metal-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001367">
        <property type="entry name" value="Fe_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036421">
        <property type="entry name" value="Fe_dep_repressor_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022687">
        <property type="entry name" value="HTH_DTXR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022689">
        <property type="entry name" value="Iron_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33238">
        <property type="entry name" value="IRON (METAL) DEPENDENT REPRESSOR, DTXR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33238:SF7">
        <property type="entry name" value="IRON-DEPENDENT TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02742">
        <property type="entry name" value="Fe_dep_repr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01325">
        <property type="entry name" value="Fe_dep_repress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00529">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47979">
        <property type="entry name" value="Iron-dependent repressor protein, dimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50944">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH dtxR-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="146" mass="17106" checksum="E65B2B2D6422A265" modified="2017-09-27" version="1">MEISKREEEYLETMYLLHKNKGVIRVKDIAKTMRVKPPSVVDALKKLADKGLVEYEKYDRILLTDEGRKVAEATYSKHLLLTQFFIDILGIPPEIAEQDACQFEHYVHDITVKRIKEFAQFVQEECPYVLKQFIKEKLAENEKASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6V0</accession>
    <name>A0A218P6V0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Indolepyruvate oxidoreductase subunit IorA</fullName>
            <shortName evidence="15">IOR</shortName>
            <ecNumber evidence="3 15">1.2.7.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 15">Indolepyruvate ferredoxin oxidoreductase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">A3L08_03815</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 15">Catalyzes the ferredoxin-dependent oxidative decarboxylation of arylpyruvates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>indole-3-pyruvate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = (indol-3-yl)acetyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12645"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17640"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57271"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="EC" id="1.2.7.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="15 16">Binds 2 [4Fe-4S] clusters. In this family the first cluster has a non-standard and varying [4Fe-4S] binding motif CX(2)CX(2)CX(4-5)CP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 15">Heterodimer of the IorA and IorB subunits.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.8" evidence="3 15"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853773.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6V0"/>
    <dbReference type="GeneID" id="33315368"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03815"/>
    <dbReference type="OrthoDB" id="19071at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043805">
        <property type="term" value="F:indolepyruvate ferredoxin oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02008">
        <property type="entry name" value="TPP_IOR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000039">
        <property type="entry name" value="Indolepyruvate oxidoreductase subunit IorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000109">
        <property type="entry name" value="Indolepyruvate oxidoreductase subunit IorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045025">
        <property type="entry name" value="HACL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017721">
        <property type="entry name" value="IorA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03336">
        <property type="entry name" value="IOR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43710">
        <property type="entry name" value="2-HYDROXYACYL-COA LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43710:SF6">
        <property type="entry name" value="INDOLEPYRUVATE OXIDOREDUCTASE SUBUNIT IORA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006439">
        <property type="entry name" value="Indolepyruvate_ferr_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 15 16" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9 15" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11 15 16" id="KW-0408">Iron</keyword>
    <keyword evidence="12 15 16" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="17">
        <location>
            <begin position="572"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="17">
        <location>
            <begin position="603"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="581"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="584"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="587"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="593"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="612"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="615"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="618"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="622"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR006439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR006439-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ASJ06515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="637" mass="70134" checksum="D0801DF342921131" modified="2017-09-27" version="1">MEATKAYPSDSAEVKTERRERKLLMGNEAIAYGALESGIAFATGYPGTPSTEVVETIARLKPEVFAEWAPNEKVALEEAAGVAYTGLRALVTMKCVGLNVAADPLMSLAYSGVEGGLVVLVADDPGPHTSQTEQDDRYYGKISLLPVLEPADPQEAHDLIKYAYELSEKYKVPIIFRTTTRVNHTTADVEVGEFVELDRKPVFKKDIERYVRASMEGNRKRHKWLNETLAKIEEEFNSMPFNWIEGKEEARIGIIVEGAPYNYVREVLPKINADFKVLKLSTPHPLPRKLVTDFLKTVDYAIVVEDGAPFLEEEVKIAAYEAGLNVPIYGKRTGHLPLEGELTPSLVRNALLKLIGEEGEEYTKPEEVAHAESLAPKRPPVMCPGCPHRGSYRAALDALRDLKLGRYKVPIHGDIGCYALSLLPPLEAIWTEYVMGASISLANGQSVVMDKKIIATIGDSTFFHNGIQPLIDAVYKNLNVLVMILDNRTTAMTGHQPHPGTGGSETGRKFNEIDIEALVKALGVKYVKTVDPYDLKATREAIKEAMQVEGPAVIIAKRECVIPVIRRGEIGEKPVVIEDKCTGCKACILLTGCPALVYDPETKKVRIDELICTGCGVCNQTCPFEAIKFPNELERGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6X8</accession>
    <name>A0A218P6X8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zinc finger ZPR1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_03875</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ZPR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853785.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6X8"/>
    <dbReference type="GeneID" id="33315380"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03875"/>
    <dbReference type="OrthoDB" id="14924at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.420:FF:000006">
        <property type="entry name" value="Zn finger containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1040:FF:000008">
        <property type="entry name" value="Zn finger containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1040">
        <property type="entry name" value="ZPR1, A/B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.420">
        <property type="entry name" value="ZPR1, zinc finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004457">
        <property type="entry name" value="Znf_ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040141">
        <property type="entry name" value="ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004470">
        <property type="entry name" value="ZPR1-like_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042451">
        <property type="entry name" value="ZPR1_A/B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056180">
        <property type="entry name" value="ZPR1_jr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042452">
        <property type="entry name" value="ZPR1_Znf1/2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00340">
        <property type="entry name" value="zpr1_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00310">
        <property type="entry name" value="ZPR1_znf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876">
        <property type="entry name" value="ZINC FINGER PROTEIN ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22794">
        <property type="entry name" value="jr-ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03367">
        <property type="entry name" value="Zn_ribbon_ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00709">
        <property type="entry name" value="Zpr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger ZPR1-type" evidence="6">
        <location>
            <begin position="20"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="197"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="236" mass="26482" checksum="78E4F5BD4DA2CBE0" modified="2017-09-27" version="1">MTEKREKELKPDDVQVISLGDCPICGGRGTLKAIQYVHDIPYFGKVMESTIICEKCGYRNADVMILEDRPPKLYTVKVEEEKDLFTRVVRSKSGTIELDEIGVKIEPGPAAEGFVTNVEGVLERVRETLLMTRHFKEQEGDDEAVKKVDEIMDYIENVKDGKKPLTIRIMDPLGNSALIGEKVKSRLLTQEEIDSLSLGPYVKVNPEEFETEGNSEGSEKSEDSSEKKPGEEASQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Y3</accession>
    <name>A0A218P6Y3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aminoacyl-tRNA synthetase class II (D/K/N) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_03985</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Y3"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03985"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006418">
        <property type="term" value="P:tRNA aminoacylation for protein translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class II (D/K/N)" evidence="5">
        <location>
            <begin position="17"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="76" mass="8173" checksum="F249E95DB6DB69C5" modified="2017-09-27" version="1">MGVYGGLRNGPRLQQDDASPEEVSFKPYLEVAKAGLLRPSAGAGIGVERLVRYIMGAKHIAEIQPFSRILGIPPVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P704</accession>
    <name>A0A218P704_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribonuclease P protein component 1</fullName>
            <shortName evidence="6">RNase P component 1</shortName>
            <ecNumber evidence="6">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Rpp29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rnp1</name>
        <name evidence="7" type="ORF">A3L08_04090</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eukaryotic/archaeal RNase P protein component 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P704"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04090"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.210">
        <property type="entry name" value="Ribonuclease P/MRP, subunit p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00754">
        <property type="entry name" value="RNase_P_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036980">
        <property type="entry name" value="RNase_P/MRP_Rpp29_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023538">
        <property type="entry name" value="RNP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023534">
        <property type="entry name" value="Rof/RNase_P-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002730">
        <property type="entry name" value="Rpp29/RNP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01868">
        <property type="entry name" value="RNase_P-MRP_p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00538">
        <property type="entry name" value="POP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101744">
        <property type="entry name" value="Rof/RNase P subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="88" mass="10244" checksum="AB95DE2037B62D0C" modified="2017-09-27" version="1">MTAKNIVWHELIGLKAKIIRASHPELVGIEGYVLDETRNTLTIGGERVWVIPKDVAELEFEVGDKRIRINGKELIGRPEMRLKKRWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P705</accession>
    <name>A0A218P705_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_04015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853809.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P705"/>
    <dbReference type="GeneID" id="33315407"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04015"/>
    <dbReference type="OrthoDB" id="85442at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050921">
        <property type="entry name" value="T4SS_GSP_E_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486">
        <property type="entry name" value="TWITCHING MOTILITY PROTEIN PILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486:SF6">
        <property type="entry name" value="TYPE IV PILUS RETRACTATION ATPASE PILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="193" mass="22110" checksum="8626608E5C888CDE" modified="2017-09-27" version="1">MGVYIFTPEDLVRYGSATQEQLEVLKEAILAKKDVLIVGSSRSGKTKLVEALMHFIPDDKKVAVITAYGEFKPFKHHVVVIDTQFDSQPLEKRTAEVISKIKAINPDYVVIDTLHTIDVARVFRELIDDYAFIVTSLALTDDIKSEVKHWLRIDDETFDKFDMVVELKRDWRTGIKGINKIYEVKNGELKLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P713</accession>
    <name>A0A218P713_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rpl22</name>
        <name evidence="11" type="ORF">A3L08_04070</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="7 9">This protein binds specifically to 23S rRNA. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7 9">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853819.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P713"/>
    <dbReference type="GeneID" id="33315418"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04070"/>
    <dbReference type="OrthoDB" id="314984at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.10:FF:000015">
        <property type="entry name" value="50S ribosomal protein L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="entry name" value="Ribosomal protein L22/L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01331_A">
        <property type="entry name" value="Ribosomal_uL22_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_uL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057265">
        <property type="entry name" value="Ribosomal_uL22_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018260">
        <property type="entry name" value="Ribosomal_uL22_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005721">
        <property type="entry name" value="Ribosomal_uL22_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_uL22_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003260">
        <property type="entry name" value="PRK04223.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01038">
        <property type="entry name" value="uL22_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593:SF10">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="Ribosomal protein L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00464">
        <property type="entry name" value="RIBOSOMAL_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7 9" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="156" mass="17782" checksum="0C3A41F0946A4991" modified="2017-09-27" version="1">MSRGRFSYSFQNFDPERMARASGRDLRISPKHSVELLREIRGMMVNDALRYLDDVIALKRPVPLRKHHDSQGHKPGKGFGPGRYPVKVAKAVKKVLLNARNNAEQKGLDVDRLKIIHAAAHRGPVLRGYIPRAFGRATPFNEQTTHIEIVVEEIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P785</accession>
    <name>A0A218P785_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribose-5-phosphate isomerase A</fullName>
            <ecNumber evidence="5">5.3.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Phosphoriboisomerase A</fullName>
            <shortName evidence="5">PRI</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpiA</name>
        <name evidence="6" type="ORF">A3L08_04555</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the reversible conversion of ribose-5-phosphate to ribulose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>aldehydo-D-ribose 5-phosphate = D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14657"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="EC" id="5.3.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Carbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the ribose 5-phosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.6" evidence="5"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853900.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P785"/>
    <dbReference type="GeneID" id="33315515"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04555"/>
    <dbReference type="OrthoDB" id="19013at2157"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00412"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004751">
        <property type="term" value="F:ribose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006014">
        <property type="term" value="P:D-ribose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01398">
        <property type="entry name" value="RPI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000018">
        <property type="entry name" value="Ribose-5-phosphate isomerase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000001">
        <property type="entry name" value="Ribose-5-phosphate isomerase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00170">
        <property type="entry name" value="Rib_5P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020672">
        <property type="entry name" value="Ribose5P_isomerase_typA_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004788">
        <property type="entry name" value="Ribose5P_isomerase_type_A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001924">
        <property type="entry name" value="PRK00702.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00021">
        <property type="entry name" value="rpiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934:SF0">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06026">
        <property type="entry name" value="Rib_5-P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75445">
        <property type="entry name" value="D-ribose-5-phosphate isomerase (RpiA), lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="26"/>
            <end position="29"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="83"/>
            <end position="86"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="96"/>
            <end position="99"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="227" mass="24932" checksum="7BF7B63B14748B73" modified="2017-09-27" version="1">MEELKRAVAKEALKFIEDDMIVGLGTGSTTAYFIEYLGKLIMEEELEDVYGVPTSYQSRMLAIENGVPVLGLDEVDAIDIAVDGADEVDPQLNLIKGRGAALTMEKIIEYRAGTFLVLVDESKLVESLGQKMPVPIEVIPAAWRAIAEEIEVFNATAELRMASKKDGPVVTDNGNFILDARFHRIEDPLDLEIELNNIPGVVENGIFADIADIVLVGTKEGVKRLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7C1</accession>
    <name>A0A218P7C1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_04720</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7C1"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04720"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="88" mass="10041" checksum="216066BFC124AE42" modified="2017-09-27" version="1">MSYITQSAVSRCLRMNRCALIEVEKLLDIDEKLRKPAETIMKKNSNEYYIHGALVKISILRCLEKVVCSFHSKSYLKLIQSSAESADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7D2</accession>
    <name>A0A218P7D2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein translocase subunit SecE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Protein transport protein Sec61 gamma subunit homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">secE</name>
        <name evidence="9" type="ORF">A3L08_04805</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Essential subunit of the Sec protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Sec protein translocase complex. Heterotrimer consisting of SecY (alpha), SecG (beta) and SecE (gamma) subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. May interact with SecDF, and other proteins may be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853941.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7D2"/>
    <dbReference type="GeneID" id="33315565"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04805"/>
    <dbReference type="OrthoDB" id="86216at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.820">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00422">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023391">
        <property type="entry name" value="Prot_translocase_SecE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008158">
        <property type="entry name" value="Translocase_Sec61-g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006909">
        <property type="entry name" value="PRK09400.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00327">
        <property type="entry name" value="secE_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103456">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01067">
        <property type="entry name" value="SECE_SEC61G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="32"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="61" mass="6687" checksum="DC95E78346A13750" modified="2017-09-27" version="1">MATTTEKLKNFFAESRRVLLVTKKPGMKEFKLAVKITGTGMILIGSVGLVIRMIGYLISGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7E8</accession>
    <name>A0A218P7E8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bifunctional protein GlmU</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_04890</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853957.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7E8"/>
    <dbReference type="GeneID" id="33315582"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04890"/>
    <dbReference type="OrthoDB" id="15372at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056764">
        <property type="entry name" value="LbH_EIF2B3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25084">
        <property type="entry name" value="LbH_EIF2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="6">
        <location>
            <begin position="2"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="EIF2B subunit epsilon/gamma LbH" evidence="7">
        <location>
            <begin position="257"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="361" mass="40147" checksum="B6AF326D45194CFD" modified="2017-09-27" version="1">MKAVVLAGGRGTRLLPLTVYRPKPMIPFFNRPLMEYALLNLINAGVDEVYVLVGYLKERIMEYFGDGSRWGIEIHYSNKDNIKLGTAGATKKVVKHMDDTFFVVSSDVLTNLDLKALYDYHRSKKALATIALSQVEDPTQYGIAIIDNDGRILRFKEKPKPEEAFSNLVNAGIYVFEPEAFDLVPKAKEFDFSKDLFPRMLENDLALYGFPFSEYWNDVGRASSYLQATEDVFLGRLILPGLRTESLKGNLEYGGAVVTGRRCILRQPEVRGFAVLGDNVEIGQNVKIERSVIFSGAVIGDGAEIREAIIGENVRIGKGVVIQPGSVIGDNTLIEDFSKIGSNVKIWVESRIGKESIVLPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7F1</accession>
    <name>A0A218P7F1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein P1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl12p</name>
        <name evidence="4" type="synonym">rpl12</name>
        <name evidence="6" type="ORF">A3L08_04830</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit. Homodimer, it forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric L10(L12)2(L12)2(L12)2 complex, where L10 forms an elongated spine to which the L12 dimers bind in a sequential fashion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853946.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7F1"/>
    <dbReference type="GeneID" id="33315570"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04830"/>
    <dbReference type="OrthoDB" id="3337at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05832">
        <property type="entry name" value="Ribosomal_L12p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022295">
        <property type="entry name" value="Ribosomal_P1_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03685">
        <property type="entry name" value="ribo_P1_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696:SF10">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="74"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="81"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="106" mass="10691" checksum="83702EF05190D8D5" modified="2017-09-27" version="1">MEYVYAALLLHAAGKEITEENLKAVLEAAGVSADEARIKALVAALEGVNIDEVIEKAAMPVAAPVAVAAAPAAEGGAAEAAAEEEEEEEEEASEEEALAGLGALFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7H9</accession>
    <name>A0A218P7H9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7H9"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05010"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="70" mass="7798" checksum="7CE4A9BF9A121B9A" modified="2017-09-27" version="1">MRRDIKRSNNSGYERTIDEYQVLIKGFLLGGTVLRVYTLRGLLLADEELPRTSGRTSYGGASGRVSYGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7I9</accession>
    <name>A0A218P7I9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Glucose-6-phosphate isomerase</fullName>
            <ecNumber evidence="5 13">5.3.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">A3L08_05190</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11816"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="EC" id="5.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the archaeal-type GPI family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.9" evidence="5 13"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854003.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7I9"/>
    <dbReference type="GeneID" id="33315642"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05190"/>
    <dbReference type="OrthoDB" id="49661at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00181"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02218">
        <property type="entry name" value="cupin_PGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016758">
        <property type="entry name" value="G6P_isomerase_archaea/bacteria"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010551">
        <property type="entry name" value="G6P_isomerase_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051610">
        <property type="entry name" value="GPI/OXD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35848:SF6">
        <property type="entry name" value="CUPIN TYPE-2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35848">
        <property type="entry name" value="OXALATE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06560">
        <property type="entry name" value="GPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019325">
        <property type="entry name" value="Glucose-6-phosphate_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="10 13" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="11 13 15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-6-phosphate isomerase prokaryote" evidence="14">
        <location>
            <begin position="25"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR019325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF06560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ASJ06751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="189" mass="21227" checksum="6858FD6D19C998F9" modified="2017-09-27" version="1">MEYKNPIGVNIDLETGVIPGAKKLVRRLSSLKGYFLDEEAYNELLKDDPVVYEVYAVEQDEKDGDLNFATTVLYPGKVGKEFFFTKGHFHSKADRAEIYYGIKGKGGMLLQTPEGKAEWIPMGPGTVVYVPPYWAHRTVNTGDEPFIFLAVYPADAGHDYGSIAEKGFSKIVIEENGEVKVVDNPRWRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7J7</accession>
    <name>A0A218P7J7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05070</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853984.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7J7"/>
    <dbReference type="GeneID" id="33315618"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05070"/>
    <dbReference type="OrthoDB" id="33550at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16018">
        <property type="entry name" value="Enpp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF120">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="372" mass="42022" checksum="653BCB3011F1CEDC" modified="2017-09-27" version="1">MRKKLALISLDGNGIYNLKHMPFLSELAETGDFAVVDSIFPTLTDLVHTSVMTGVWPKDHGVVENGYYDRLSDRKVNFYEYEVAFNPHRVIKAPTIVDILRGRGVRTAAVSGYTMPPFSGTDVRIFPPFFAGDKMYRQHGRDWRKDVWVLNSALYLYEECKPDLLLVHFASIDGMSHDHGPLSEGALKAVETVDTAVRTLWERLKDEYAFIIFADHGQEEVHTWVNLRAYLRKHGIETLRVSSGGGVHVYLRDPNQSEEAFEVLRKAPGVREVFFRDELPHLDSPNSGELIVSAKPGYWFCSHRMCKGTKGVSHWVKGMHGSMNEPVVKVPLILWGFENANLENASLMDIAPTILDFFGVEKPTNMVGKSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7K1</accession>
    <name>A0A218P7K1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cobalt ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_05165</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853998.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7K1"/>
    <dbReference type="GeneID" id="33315637"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05165"/>
    <dbReference type="OrthoDB" id="51610at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16914">
        <property type="entry name" value="EcfT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003339">
        <property type="entry name" value="ABC/ECF_trnsptr_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051611">
        <property type="entry name" value="ECF_transporter_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34857">
        <property type="entry name" value="SLL0384 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34857:SF2">
        <property type="entry name" value="SLL0384 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02361">
        <property type="entry name" value="CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="219" mass="24604" checksum="76395D88FABC273A" modified="2017-09-27" version="1">MYLPFIFLYAIGVVTRKSLTELAYFALLFLVVALIMRPKRSVFKKLGFLLGFEGLLFVMALFNPGRPLLETPLGPITHEGVYSFFTLLGKAFLSAGTAVVVTDSVGFSRILAEMEALRFPRVLTLTLAFTYRYLDLFVEEAMRMKRALDSRTFGVGKREYYRKLGSLIGEVFVRAYLRNGRIYNAMLSRGFGEFPSLEEPRPSVQTATLVLLALGGLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7P1</accession>
    <name>A0A218P7P1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hydrogenase formation protein HypD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_05345</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HypD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854033.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7P1"/>
    <dbReference type="GeneID" id="33315673"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05345"/>
    <dbReference type="OrthoDB" id="372075at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070025">
        <property type="term" value="F:carbon monoxide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11740">
        <property type="entry name" value="HypD, alpha/beta domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002780">
        <property type="entry name" value="Hyd_form_HypD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042243">
        <property type="entry name" value="HypD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042244">
        <property type="entry name" value="HypD_2_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00075">
        <property type="entry name" value="hypD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30149:SF0">
        <property type="entry name" value="HYDROGENASE MATURATION FACTOR HYPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30149">
        <property type="entry name" value="HYDROGENASE PROTEIN ASSEMBLY PROTEIN HYPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01924">
        <property type="entry name" value="HypD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005622">
        <property type="entry name" value="Hydrgn_mat_hypD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="369" mass="41514" checksum="A3EA3D3EA37F3100" modified="2017-09-27" version="1">MEPLSVFKDRKIAQRIVRQIHEEAKGLEEVRFMHVCGTHEDTVTRFGIRSLLPENVKIVSGPGCPVCITPVEDIVKMREIMKEAYAEGDRIILTTFGDMYKIPTPLGSFADLKSEGYDVRIVYSIFDTYRIAKENPDRTVVHFSPGFETTTAPTAGMLNTVVEEGLENFRIYSVHRLTPPAVEALVKQGTKFHGLIDPGHVSTIIGVKGWEYLTGDYGIPQVIAGFEPVDVLMGILMLIRMVKSGEVKILNEYTRAVRYEGNVTAQRLIEKFFEVKDAKWRALGVIPKSGLELKREWRELEIRTYYDPEVPKLPDLEKGCLCGAVLRGLALPTDCPHFGKTCTPRSPVGPCMVSYEGTCQIFYKYGVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Q1</accession>
    <name>A0A218P7Q1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05525</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854067.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Q1"/>
    <dbReference type="GeneID" id="33315709"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05525"/>
    <dbReference type="OrthoDB" id="92924at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="275" mass="31522" checksum="C4C0E83CFB8637C8" modified="2017-09-27" version="1">MGKLDEFFRDPRGEKKRSELEVLEEIESYLSEGRIDDTIPLLEALGKEHNLYLALRMIIRTITESLDEAEKNGGLTDYELEKTKERIKNLVPAVNGLFNKRYRALLLSDLAVLLYRLNDELNGDMALRAAINLAGDEANIVRDILMELIRRGLLDKAGYAMKMVKDPEKLDVVLTHIAEGFYLAGDVEKAMLILRHITSPFHRAMALYYMASIEGQRNREKALKLIDAAFKEAEKVEDPDARFELSLKLYDLKHSILGEGFNVRDILEWREAPQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7T1</accession>
    <name>A0A218P7T1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05730</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854105.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7T1"/>
    <dbReference type="GeneID" id="33315750"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05730"/>
    <dbReference type="OrthoDB" id="7902at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.280">
        <property type="entry name" value="AF1531-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007003">
        <property type="entry name" value="DUF655"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40734:SF1">
        <property type="entry name" value="DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40734">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04919">
        <property type="entry name" value="DUF655"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160975">
        <property type="entry name" value="AF1531-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="206" mass="24518" checksum="E5000F42B25BE800" modified="2017-09-27" version="1">MDRYRRHSYRESLDKKKRNVEYEEYAYVLDYLPEGYTDLTTGRRTGKPVAQVIGEKAFTLLEVAPKEDLMLYERVFIGKGPRDKILMINKKIHYDDLTPTAKAELPYVVEEIIKNNEERFVQFFNMAPPITNRLHSLELLPGIGKKHMWEILDERKREPFKSFKDLHERVKGLPDPVKMITKRVVEEIEGKDRYRLFVGSRRLFRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7U9</accession>
    <name>A0A218P7U9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo4</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit F</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo4</name>
        <name evidence="1" type="synonym">rpoF</name>
        <name evidence="2" type="ORF">A3L08_05725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit is less well bound than the others.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the RNA polymerase complex. Forms a stalk with Rpo7 that extends from the main structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic RPB4 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854104.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7U9"/>
    <dbReference type="GeneID" id="33315749"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05725"/>
    <dbReference type="OrthoDB" id="25158at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.80">
        <property type="entry name" value="HRDC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00864">
        <property type="entry name" value="RNApol_arch_Rpo4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005574">
        <property type="entry name" value="Rpb4/RPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010924">
        <property type="entry name" value="Rpo4"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011551">
        <property type="entry name" value="PRK14981.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39646:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT RPO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39646">
        <property type="entry name" value="RNA POLYMERASE RPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03874">
        <property type="entry name" value="RNA_pol_Rpb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005053">
        <property type="entry name" value="RNA_pol_F_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="122" mass="14630" checksum="E122F7A0C052777D" modified="2017-09-27" version="1">MIGRKKLEEHYLTIAETKELLERRKAEGMEENPEEPMFYEARVSLEHAERFAKLKPEQAAELKEKLLGLFDWIDERIAVKLIDLLPEDYFDIRVIFAKEDYMPTKEEAEEIIRLLDDYRPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7W0</accession>
    <name>A0A218P7W0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05840</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854127.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7W0"/>
    <dbReference type="GeneID" id="33315772"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05840"/>
    <dbReference type="OrthoDB" id="85908at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="23"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="192" mass="21807" checksum="9D674B7C090B8359" modified="2017-09-27" version="1">MPLKLPIFRKKVLGLLASTDETKVMHISDTPESSYRFIEDLIDKTSPDYIIHTGDLVDNVKLERRPDLLPIYRGALRKLARVLKGSGAKLYVVPGNEDDPELVREFFGDAVVEPGSVIEIEGVRFALGHTWEDVVDKDADFRLYGHNFKLIDRGLNGVLGVNFVLLPSRRTYRVKYPGGTDFDRGYKMWRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7W8</accession>
    <name>A0A218P7W8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854124.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7W8"/>
    <dbReference type="GeneID" id="33315769"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05825"/>
    <dbReference type="OrthoDB" id="86283at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="315"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="354"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="377"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="406"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="436"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="459" mass="49759" checksum="3A1ADDC28C323448" modified="2017-09-27" version="1">MRKISFLMAFLITGYILGIWNFLVLPKYYIAFGLKGFLVSLIPTLAAMYLIYSEAESTKKTRYLIYELFFKVSRTPALIFTLLIFLVIMLGITTYYSAYGLAFIVGGSSSYILAMAIGTILLAVVLLMMAKGRTLEFISVMSILFILLAISAAFLIREQALSTVTSPQAAQYMDKALSSITSFNLPLTVKGVLYLVVSTFISLGLGAGVYYVIGSFTPEELDLKKVLAGVFILQIIISFAAAFTVGYSLGAAYEGFQKAFQNPNIPAEESMKLFMKFNDLKDYATNSEKSPMDSIEVFYSIPQILKGNVANDTSLIVLLMLSLYLAGLTTIIVLIEMGSQILSEVMQFGRNQSLGFVGLIGMVIAGAMIIADIRTMFVVVPFAVGALVAAVETYPLLSSELAHNKAVVTGILLLLVATGLATLYSSLTASATTVKIGALLGVVLFVPVLMNGMLLKSRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7W9</accession>
    <name>A0A218P7W9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2,3-bisphosphoglycerate-independent phosphoglycerate mutase</fullName>
            <shortName evidence="7">BPG-independent PGAM</shortName>
            <shortName evidence="7">Phosphoglyceromutase</shortName>
            <shortName evidence="7">aPGAM</shortName>
            <ecNumber evidence="7">5.4.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">apgM</name>
        <name evidence="9" type="ORF">A3L08_05890</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 7">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 7">Belongs to the BPG-independent phosphoglycerate mutase family. A-PGAM subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.12" evidence="7"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854137.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7W9"/>
    <dbReference type="GeneID" id="33315782"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05890"/>
    <dbReference type="OrthoDB" id="52918at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00186"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004619">
        <property type="term" value="F:phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16011">
        <property type="entry name" value="iPGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2130">
        <property type="entry name" value="Metalloenzyme domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01402_A">
        <property type="entry name" value="ApgM_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023665">
        <property type="entry name" value="ApgAM_prokaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004456">
        <property type="entry name" value="Pglycerate_mutase_ApgM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042253">
        <property type="entry name" value="Pglycerate_mutase_ApgM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00306">
        <property type="entry name" value="apgM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003104">
        <property type="entry name" value="PRK04024.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209">
        <property type="entry name" value="COFACTOR-INDEPENDENT PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209:SF0">
        <property type="entry name" value="METALLOENZYME DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10143">
        <property type="entry name" value="PhosphMutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006392">
        <property type="entry name" value="IPGAM_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloenzyme" evidence="8">
        <location>
            <begin position="5"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="411" mass="45377" checksum="EBB6E520979B7EA0" modified="2017-09-27" version="1">MKQRKGLLIILDGLGDRPIKEFGGKTPLEYANTPNMDRLAKMGILGQQDPIKPGQPAGSDTAHLSIFGYDPYKVYRGRGYLEAMGVGLDLSEDDLAFRVNFATIENGIITDRRAGRISTEEAHELAKAIQENVKIPVDFIFVGATGHRAVLVLKGMAKGYRVGENDPHEAGKPPHRFNWEDEESKKVAEILEEFVRKAHEVLDKHPINEKRRKEGKPVANYLLIRGAGTYPGIPMKFTEQWKVKAGAVIAVSLVKGVARAIGFDVYTPEGATGEYNTNEMAKARKVIELLKDYDFVFLHFKPTDAAGHDNNPKLKAEMIEKADRMIGYIMDNIDLEDVVIAITGDHSTPCEVMNHSGDPVPLLIAGGGVRPDSTEAFGERECMRGGLGRIKGHDIVPIMMDLMNRSEKFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7X9</accession>
    <name>A0A218P7X9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Deoxyribonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7X9"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05875"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRAM" evidence="1">
        <location>
            <begin position="12"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="70" mass="7842" checksum="B244BB5227FA449C" modified="2017-09-27" version="1">MERKSVPSKKPPVRRGERYKVRIETLGKGGDGIARIKGFVIFVPKTNVGDEVQIEIKNVKERFAFGEVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Z0</accession>
    <name>A0A218P7Z0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphatidylglycerophosphatase A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05915</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854142.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Z0"/>
    <dbReference type="GeneID" id="33315787"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05915"/>
    <dbReference type="OrthoDB" id="53211at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008962">
        <property type="term" value="F:phosphatidylglycerophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3760.10">
        <property type="entry name" value="PgpA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036681">
        <property type="entry name" value="PgpA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017577">
        <property type="entry name" value="Ribazole_CobZ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03161">
        <property type="entry name" value="ribazole_CobZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101307">
        <property type="entry name" value="YutG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="138" mass="15288" checksum="CC7E5D8BBC7EED43" modified="2017-09-27" version="1">MKAEELLQRLEKHGITLEKMLDTAMGLYIGDNPEEAREMLGRLMLHYLGDINVQSLLMAALLLEESFKVEGDPVNLVADELIGIDIAEYIGGKMALFNFFYYDTKKPGILAELPPFLDDAIGGFIAGCMTKLFQEGVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P800</accession>
    <name>A0A218P800_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P800"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06025"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="66" mass="7661" checksum="BDD68E0946389D14" modified="2017-09-27" version="1">MESKLTYVLLVLVIISLLLNGVLLYALFQVRNIATREIDNVVEMVEGVKEQKITFDYPVDQETRYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P803</accession>
    <name>A0A218P803_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">tRNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_05975</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P803"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05975"/>
    <dbReference type="OrthoDB" id="14546at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02153">
        <property type="entry name" value="tRNA_bindingDomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041169">
        <property type="entry name" value="Alpha_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051270">
        <property type="entry name" value="Tyrosine-tRNA_ligase_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586">
        <property type="entry name" value="TRNA-AMINOACYLATION COFACTOR ARC1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586:SF37">
        <property type="entry name" value="TRNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18489">
        <property type="entry name" value="Alpha_Helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="4">
        <location>
            <begin position="132"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="241" mass="26685" checksum="54311FBF57E30B65" modified="2017-09-27" version="1">MWDTSKDYRLLVAEKSVELFLKTIEHAKFKGKWNKKGAQQLAKEMLPEIQAMRYSYVEPKELIETPQMKALKEKANGIIEALGGEDWHHKFLSLADKSEREKVEEAVAKVRFFLNTILGLDRRLALGKINDPVIAVDIKVGEVMSVAKHPNADRLLVTNVNIGDRAITVVTNDLTVKEGNRVAVALLPPANFRGIVSEGMFLGAGEGVLKDVKGEIGGLPKGIPLEAFNETRNLVEAFLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P814</accession>
    <name>A0A218P814_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Potassium transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">A3L08_05805</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854120.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P814"/>
    <dbReference type="GeneID" id="33315765"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05805"/>
    <dbReference type="OrthoDB" id="12029at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562">
        <property type="entry name" value="NAPA-TYPE SODIUM/HYDROGEN ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562:SF3">
        <property type="entry name" value="SODIUM ION_PROTON EXCHANGER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="102"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="174"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="228"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="305"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="12">
        <location>
            <begin position="15"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="408"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="439" mass="47529" checksum="5314051D7730CD62" modified="2017-09-27" version="1">MEGVAWLLATIGIALILAKIGDSIIERFELPGVLGELIMGMLLGNLVYFGFVAPQYLPIVSGQAFTTDVSQISEFLAKLGIIFLLFLGALDTDVEQLKKTGVTATVSTLLGVFVPLILGWYALIAMGYPSREAFAGGVLLTATSIGLTVRVMMDLGVLRSEVGAASLSASVMDDFLGIALVIFAVGSGSLIDLSVKIVAFFIITGVLGWYFIDYYIRFAERLHVEKGILGMTIGMMFLFAALAEGWFAAAIEGAFMMGLILSKLPEGKRLMEDVRAIGYGLLIPFFFVHTGAMLNLTVFENANALILAAVMTTIAVLGKIIGRGFGAWITAWGRGKDFLFTKENFWMSLQMGIGSVPRTEVALVDLMVAIHGGAISPDHAQEFIAATLMFITISVLITPPLLKWAFRREIEEAKATKASKKVERIQETKEKIKELKGSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P819</accession>
    <name>A0A218P819_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein eL43</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">rpl37ae</name>
        <name evidence="6" type="ORF">A3L08_06050</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the eukaryotic ribosomal protein eL43 family. Putative zinc-binding subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854164.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P819"/>
    <dbReference type="GeneID" id="33315813"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06050"/>
    <dbReference type="OrthoDB" id="372011at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00327">
        <property type="entry name" value="Ribosomal_eL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050522">
        <property type="entry name" value="Ribosomal_protein_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00280">
        <property type="entry name" value="eL43_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003058">
        <property type="entry name" value="PRK03976.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="1 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="zinc finger region" description="C4-type" evidence="5">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="86" mass="9177" checksum="E92DB256DB7DC700" modified="2017-09-27" version="1">MGRTTKVGSAGRFGPRYGLKIRRRVAAVEAKMKQKHVCPVCGRNAVRRISTGIWQCQKCGATFAGGAYLPTTPAGKVAKRVTASKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P820</accession>
    <name>A0A218P820_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cation:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_06225</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854195.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P820"/>
    <dbReference type="GeneID" id="33315848"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06225"/>
    <dbReference type="OrthoDB" id="84883at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007208">
        <property type="entry name" value="MrpF/PhaF-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009250">
        <property type="entry name" value="PRK12604.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34702">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER SUBUNIT F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34702:SF1">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04066">
        <property type="entry name" value="MrpF_PhaF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="84" mass="9270" checksum="396BE70E83165921" modified="2017-09-27" version="1">MIGINVYLALIAIATLLSMYRVFRGPTTVDRLVAVDIMTTITTGLMVLFALYYKRMIFLDVALVYAILAFGGVIAFARYLEGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P823</accession>
    <name>A0A218P823_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10 11">Ornithine carbamoyltransferase</fullName>
            <shortName evidence="10">OTCase</shortName>
            <ecNumber evidence="3 10 11">2.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">A3L08_05855</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>carbamoyl phosphate + L-ornithine = L-citrulline + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.3" evidence="3 10 11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854130.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P823"/>
    <dbReference type="GeneID" id="33315775"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05855"/>
    <dbReference type="OrthoDB" id="4696at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004585">
        <property type="term" value="F:ornithine carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019240">
        <property type="term" value="P:citrulline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000008">
        <property type="entry name" value="Ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000016">
        <property type="entry name" value="Ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01109">
        <property type="entry name" value="OTCase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002292">
        <property type="entry name" value="Orn/put_carbamltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024904">
        <property type="entry name" value="OTCase_ArgI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00658">
        <property type="entry name" value="orni_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001986">
        <property type="entry name" value="PRK00779.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753">
        <property type="entry name" value="ORNITHINE CARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753:SF3">
        <property type="entry name" value="ORNITHINE TRANSCARBAMYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00102">
        <property type="entry name" value="OTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase carbamoyl-P binding" evidence="14">
        <location>
            <begin position="8"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase Asp/Orn-binding" evidence="13">
        <location>
            <begin position="155"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="57"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="135"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="234"/>
            <end position="235"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="270"/>
            <end position="271"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="NCBIfam" id="TIGR00658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ASJ06880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="315" mass="34960" checksum="CB359BAA4DC1CE9C" modified="2017-09-27" version="1">MVVSLAGRDVLCLQDFTREEIETILKTAEMMKIWNKIGKPHRVLEGKTLAMIFQKPSTRTRISFEVGIYQLGGYGLYLNAQDLQLRRGETIADTARVLSRYVDGIMARVYAHKDVEDLAKYASVPVINGLSDFSHPCQALADYQTILEKKGRIAGLKIVYVGDGNNVAHSLMIAGTKLGANVVVATPEGYEPDPKVVKWAEQNAAESGGSFELLHDPVKAVKDADVIYTDVWASMGQEAEAEERRKIFMPFQVNRDLVKHAKPDYIFMHCLPAHRGEEVTDDVVDSPNSVVFDEAENRLHAQKALMALVMGGIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P865</accession>
    <name>A0A218P865_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854170.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P865"/>
    <dbReference type="GeneID" id="33315819"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06080"/>
    <dbReference type="OrthoDB" id="102356at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="100" mass="11397" checksum="975FB2957EB40573" modified="2017-09-27" version="1">MRRLYWAVPFRLFLAAYLFWSLTLPALVVTLLNWGTFLLEYRCGGESKEAEELVVVGLVTSSALIVLEEELFRVLAVVEAFSLFLLEFTAAFFKLKVRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8C9</accession>
    <name>A0A218P8C9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribonuclease VapC</fullName>
            <shortName evidence="5">RNase VapC</shortName>
            <ecNumber evidence="5">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Putative toxin VapC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">vapC</name>
        <name evidence="8" type="ORF">A3L08_06460</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Toxic component of a toxin-antitoxin (TA) system. An RNase.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PINc/VapC protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="5"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854239.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8C9"/>
    <dbReference type="GeneID" id="33315895"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06460"/>
    <dbReference type="OrthoDB" id="147997at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18677">
        <property type="entry name" value="PIN_MjVapC2-VapC6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00265">
        <property type="entry name" value="VapC_Nob1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022907">
        <property type="entry name" value="VapC_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39677">
        <property type="entry name" value="RIBONUCLEASE VAPC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39677:SF4">
        <property type="entry name" value="RIBONUCLEASE VAPC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0800">Toxin</keyword>
    <keyword evidence="1 5" id="KW-1277">Toxin-antitoxin system</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="PIN" evidence="7">
        <location>
            <begin position="2"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="143" mass="15756" checksum="B7B59B3742618DC4" modified="2017-09-27" version="1">MKGVLLDTSILIEHFKGNLKARNVLLSLMDSDVVLFINPIVFSEVVYLLLGFYSGVSPRSLKGKPEKLPGELDLVFESLEEYAFVELGEKTSRIARDLIRKYAMLPNDALILATCIEHGFSLATLDEDFKAPAGKEGVPIITG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8D6</accession>
    <name>A0A218P8D6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Methionine--tRNA ligase</fullName>
            <ecNumber evidence="15">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Methionyl-tRNA synthetase</fullName>
            <shortName evidence="15">MetRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">metG</name>
        <name evidence="19" type="ORF">A3L08_06740</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 15">Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 15">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="15"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854288.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8D6"/>
    <dbReference type="GeneID" id="33315951"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06740"/>
    <dbReference type="OrthoDB" id="371856at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07957">
        <property type="entry name" value="Anticodon_Ia_Met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02800">
        <property type="entry name" value="tRNA_bind_EcMetRS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.20:FF:000001">
        <property type="entry name" value="Methionine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000042">
        <property type="entry name" value="Methionine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.20">
        <property type="entry name" value="Methionyl-tRNA synthetase, Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00098">
        <property type="entry name" value="Met_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041872">
        <property type="entry name" value="Anticodon_Met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004495">
        <property type="entry name" value="Met-tRNA-synth_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023458">
        <property type="entry name" value="Met-tRNA_ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029038">
        <property type="entry name" value="MetRS_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00399">
        <property type="entry name" value="metG_C_term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001100">
        <property type="entry name" value="PRK00133.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765">
        <property type="entry name" value="METHIONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765:SF1">
        <property type="entry name" value="METHIONINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19303">
        <property type="entry name" value="Anticodon_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57770">
        <property type="entry name" value="Methionyl-tRNA synthetase (MetRS), Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 15 17" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="10 15 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 15 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 15 17" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15 16" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 15 16" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="9 15" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="TRNA-binding" evidence="18">
        <location>
            <begin position="644"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="15">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="15">
        <location>
            <begin position="345"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="348"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ASJ07038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="744" mass="86617" checksum="515F1C1731791DF8" modified="2017-09-27" version="1">MVRYMVTSALPYANGPIHAGHLAGAYLPADIFVRYLRLKGEEVLFICGTDEHGTPITFRALKEGRSPREIVDEFHEHIKTTFERAKISFDFFGRTELPVHYRLSQEFFLKALENGHLVKKVSKQAYCEHDKMFLPDRYVIGTCPYCGAENQRGDQCEVCGHPLTPEILINPRCNICGNPVTFKGSAHYYIKMQDFQERLKKWVESQEHWKPNVRNTVLGWINEGLEERAMTRDLDWGIPVPLDEEDVRGKVLYVWFEAPIGYISITIEHLKREGRENEWKKFWLNLDGQTKVIHFIGKDNVPFHAIFWPAFLMAYGKYKDEEVEAEWNLPYDIPANEYLNLEGRKFSTSRNWAIWVHEFLDAFPADYLRYYLTAIMPETRDSDFNFADFKSKINEELVNNLGNFVHRAMTFVNRYFDGIVPERGELDDLDRQAFEEIEKAFEEVGELIMNYRFKDALKRVMELAIFGNRYFDYQKPWKTAKTDRERTATTVNVSLQIVKALGILLEPFLPDASEKIWHLLNLEESKSWEFSELKAGHRVRKAFPMFRKVTDEEIIHFIVNYIGRGNPESAKLLLEKYYKKDDVFKVILERFGEERKKEALALLEAIYKEVPEVKGGKAEKAKSPGKKENAKKEGKKMEYISFDDFAKLDLRVGKIIEVKDHPNADRLYVVKVDLGDEVRQLVAGLKKYYKPEELLNHYVVIIANLEPKKLRGVESQGMLLAADDGENVALLMPDKEIKLGSRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8D7</accession>
    <name>A0A218P8D7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">6-pyruvoyl tetrahydropterin synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_06745</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854289.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8D7"/>
    <dbReference type="GeneID" id="33315952"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06745"/>
    <dbReference type="OrthoDB" id="6529at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.10">
        <property type="entry name" value="6-pyruvoyl tetrahydropterin synthase/QueD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007115">
        <property type="entry name" value="6-PTP_synth/QueD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038418">
        <property type="entry name" value="6-PTP_synth/QueD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589:SF7">
        <property type="entry name" value="6-PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589">
        <property type="entry name" value="PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01242">
        <property type="entry name" value="PTPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006113">
        <property type="entry name" value="PTP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR006113-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="115" mass="13368" checksum="68A6C591860D2C10" modified="2017-09-27" version="1">MKSRVIERFKFEAAHAVVIDGQAEEIHGHTFRLEIAVEGPLRNGYVIDFLQLRAIVEDILEQLDHRNLNTFFDNPTTENIALWIAGEVEKRLPEGVKLHRLRLWEGDENGVEFEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8E5</accession>
    <name>A0A218P8E5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Signal recognition particle receptor FtsY</fullName>
            <shortName evidence="8">SRP receptor</shortName>
            <ecNumber evidence="8">3.6.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ftsY</name>
        <name evidence="11" type="ORF">A3L08_06815</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.4"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the GTP-binding SRP family. FtsY subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.4" evidence="8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854302.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8E5"/>
    <dbReference type="GeneID" id="33315966"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06815"/>
    <dbReference type="OrthoDB" id="372188at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005047">
        <property type="term" value="F:signal recognition particle binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17874">
        <property type="entry name" value="FtsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000053">
        <property type="entry name" value="Signal recognition particle receptor FtsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00920">
        <property type="entry name" value="FtsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004390">
        <property type="entry name" value="SR_rcpt_FtsY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00064">
        <property type="entry name" value="ftsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134:SF1">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="10">
        <location>
            <begin position="301"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="2"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="140"/>
            <end position="147"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="222"/>
            <end position="226"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="280"/>
            <end position="283"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ07052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="330" mass="36627" checksum="CF547EC72B02A098" modified="2017-09-27" version="1">MFGKLREKLKKFTQTVEEKIEEEEKVVEKKTPEETEEKKGFIERLLQVEIKEKDIEDALDELELELLEADVALETVEALREKIKEKLVGRKVRVGTNKGKIIEEALREAITEILTPEKRIDLIEMIKSKEEKPFVIAFVGFNGSGKTTTIAKLAHGLKKNGLSVVIAASDTFRAGAIEQLEEHAKRVGVKVIKHSYGADPAAVAYDAIQHAKARGIDVVLIDTAGRNELNRNLMDEMKKIARVTKPDLVIFVGDSLGGNSVVEQAKQFNEAVRIDGVILTKLDADARGGAALSISHAIGAPILFVGVGQGYDDLRPFDEKWFVERIFGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8M0</accession>
    <name>A0A218P8M0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_07015</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854340.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8M0"/>
    <dbReference type="GeneID" id="33316006"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07015"/>
    <dbReference type="OrthoDB" id="49590at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010624">
        <property type="entry name" value="KaiC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637:SF1">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01874">
        <property type="entry name" value="DNAREPAIRADA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51146">
        <property type="entry name" value="KAIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KaiC" evidence="3">
        <location>
            <begin position="4"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="234" mass="26371" checksum="627C538BA3F4A8EC" modified="2017-09-27" version="1">MTIQRISTGIQGLDVMLQGGLIPGRVYLVKGAPGTGKTTLAMHFAMAGVENGEDVLYVTIEEPAENLKVDMSKLGFNLRDPRFSLIDATPTAERYVLITDFFEEFAGNIEKMTDAIKRQFQERNYTRVVIDPITMLKLTATKEIDYRKAFLSFIKSMMRLRATVLLTSELEKTDIEEYLVSGVIELKAFNAGGRLTRGVRIIKFRGSSFDGTIRPYEITDRGIVVYHDRMISLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8U5</accession>
    <name>A0A218P8U5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sugar dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854445.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8U5"/>
    <dbReference type="GeneID" id="33316124"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07605"/>
    <dbReference type="OrthoDB" id="35501at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd05344">
        <property type="entry name" value="BKR_like_SDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050259">
        <property type="entry name" value="SDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006213">
        <property type="entry name" value="PRK08340.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879:SF5">
        <property type="entry name" value="SHORT-CHAIN ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="268" mass="29735" checksum="FC849BD633880BCD" modified="2017-09-27" version="1">MSVKIDLDGLGVIVTASSRGIGFNVARELLRRNARVVISSHSLENLKKAEEELSEFGEVHAVQANLFRQEEVENLVKEAWELLGGVDALVWNAPNVPCEPCLLHEAGYLDWIEASALHTVAPGYLTTLLVQTWLEKRMKGRLVYLNSVSVKEPMPPLVLADVTRAGLVQLAKSVSRTYGKYGIRAYSVLLGSFDTPGARENLRALAEERGEPFEETWEREVLERTPLHRTGRWSELGSLVAFLLSDEAEYMLGSTVVIDGAMTRGVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8V9</accession>
    <name>A0A218P8V9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07695</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854458.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8V9"/>
    <dbReference type="GeneID" id="33316142"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07695"/>
    <dbReference type="OrthoDB" id="90406at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="278" mass="31248" checksum="3CCB635B9030F585" modified="2017-09-27" version="1">MGENIPSLKKVAEAKNSPEELYIPKGVRFKKKTFLRASGYVFLIMLVLGMVLIGYGASLDPTVKYVVADRWDKSWELKPGYYNHHTWKLESPHDAFLEINATVSGGNGDLDVYIDTPSGRVNYGRLTNPVHVTVNLSRYGVGEYTIYLDNSFSVVTSKYITLHEAVYRKVQDTTDKDAYQALGGILMFVGFFGVLYTLSKRVILEVDGDEIVMEFKSGLLKNYVELTVNGFKLDQKIKGPVKFRVGQNEDKVLEIEPIVFLNLAWKITLDGQFIGGVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8W0</accession>
    <name>A0A218P8W0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Twitching motility protein PilT</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854431.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8W0"/>
    <dbReference type="GeneID" id="33316109"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07530"/>
    <dbReference type="OrthoDB" id="275611at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09874">
        <property type="entry name" value="PIN_MT3492-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="6"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="153" mass="17461" checksum="9863AF6E88D3CCB5" modified="2017-09-27" version="1">MTGRSVYLDTSAILKRYLDEEGSDVVKELFRDAYRGEVRLAFSFWNIGEVIGVLDKRLRREQLSKDEFDFLKKGFLAEVKRFTRLGVLEVVPVHSLILADAWKLIEKYHMYQADALQIVSAKYVEAGSFYTADKRLHQVAIEEGLNSILLGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8X5</accession>
    <name>A0A218P8X5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Prenyltransferase alpha-alpha toroid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_07820</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854480.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8X5"/>
    <dbReference type="GeneID" id="33316168"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07820"/>
    <dbReference type="OrthoDB" id="84819at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00688">
        <property type="entry name" value="ISOPREN_C2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016648">
        <property type="entry name" value="UCP016175_prenyltrans-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016175">
        <property type="entry name" value="UCP016175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="2">
        <location>
            <begin position="15"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="2">
        <location>
            <begin position="133"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="292" mass="32902" checksum="B7F4586CA0952396" modified="2017-09-27" version="1">MGSKLYELRRYINVKGILDYIEERRHDDGGYCFVSVLDDTNVNDTYYAVKIYDLLGLEVPNGEKTIEFFEEAIQPQTAVVAIAMALEGLALLGAKDVAVEHTDIVFTKYNPLEGKFAVGLGGSEEFGTATPLEATYWVVKAFRAIGYEFSPEEKEAIREFVMRFRNGNGYGVKQPTTTMTYQALYTLNALGYMPPKSSHFKNCELCGDWGGFTEVPYSLPPYLEPTFYATRGLELQGEMPSCPKRHVWFIRQLQNSNGGFRRSLELGISNFQNTYRALAVVEAMGLNLFKDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Z1</accession>
    <name>A0A218P8Z1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fe/B12 periplasmic-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854513.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Z1"/>
    <dbReference type="GeneID" id="33316203"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07990"/>
    <dbReference type="OrthoDB" id="24039at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01147">
        <property type="entry name" value="HemV-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050902">
        <property type="entry name" value="ABC_Transporter_SBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30535:SF34">
        <property type="entry name" value="MOLYBDATE-BINDING PROTEIN MOLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30535">
        <property type="entry name" value="VITAMIN B12-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53807">
        <property type="entry name" value="Helical backbone' metal receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="1">
        <location>
            <begin position="51"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="372" mass="40775" checksum="1F732A4C3521E218" modified="2017-09-27" version="1">MRKLLSLFLIVLVVSLSGCIGSMNTGGTGKETVTVTDALGRTVEVPAKVTKVVAAGPGALRLIVYLNASDMVVGVEDFEKRYNFGRPYIIAHPELKELPSIGPGGPGKLPDVEALINLKPDVIFLTYVDVKTADDIQEKTGIPVVVLSYGELATFDDEELFKSLELAGRILGKEERAKEVINFIKSVQDDLSKRTEGVEPKKVYVGGIGYKGAHGIESTEGKYPPFVAVRADNVADELGKGHHSIDKEKLLEWQPDYIFIDEGGLKLLLDDYRKNPDFYSSLKAIKNGNVYGLLPFNFYTTNIGTALADAYYIGKVLYPERFSDVDPAEKADEIYTFLVGKPAYREMAEQFGGFGKIDLENGTVKYSLPTSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Z3</accession>
    <name>A0A218P8Z3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bacterial Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_07925</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854500.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Z3"/>
    <dbReference type="GeneID" id="33316190"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07925"/>
    <dbReference type="OrthoDB" id="100843at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046878">
        <property type="entry name" value="Big_14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20251">
        <property type="entry name" value="Big_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Bacterial Ig-like" evidence="2">
        <location>
            <begin position="52"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="149" mass="16344" checksum="1049E289B6AC5222" modified="2017-09-27" version="1">MRKVIPVLLIILLVPVGYYFASYWGSPADGSSGGKDLTHTSPDSGLGTETLLKLDKTVYSPNDTMLITITNKGDGNITTGYAFRLYRLENGTWNEVPVNLVFIEVAVVIEPGKSWEQKVNLADLKLDQGHYRIVKTVSVTDPVTKMALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P915</accession>
    <name>A0A218P915_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycogen debranching enzyme C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P915"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08100"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032790">
        <property type="entry name" value="GDE_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06202">
        <property type="entry name" value="GDE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycogen debranching enzyme C-terminal" evidence="1">
        <location>
            <begin position="292"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="588" mass="66645" checksum="EDCE08F2AC4BB19C" modified="2017-09-27" version="1">MNQKAYIKSLNYRCGGSTMRHVISSGHEFLVFKAEEGTVDFYAYDTLFLSGIRLSTSVPLEPLEITRSSDEVVITYSGEGIRAVRRVKLGGGYRERVIFFSRADDERVIEGRYVFTVPMADILERDYPQASPQRKIEREGNVYAYLGMDGRRRTVRISGESFRVSIPPRGFGRIEFSATPFVEGEFLLPTFRKASFQFNWKPLERVESAVYLRAIEDLEQLIVDIDGYPFPLAGLPDFGAVFGRDSIWTALFLLEEYPQIARGVLKVLSRLQGKRFNAKTEEEPGKIPHEFRFGELCQAGVIPFNPYYGTVDATPLYVALAGEYLKRTEDRAFLDSIKNNLNMAVEWILRRLEEGGGYIRYDKTPLGLENQGWKDWKNSIPDENGVQVKHPVAVVEVQGYAYLALKTASELNLTDYDEKMLEKTAERLKRRFNKEFWTGEHYGIALDGENRLSKVVSSNMGHLLITGILPEERARAVAERLIEGDMFSGWGIRTLSSKERAYDPFSYHNGSVWPHDNAIIVLGLWKAGFKNQALKLASSLLNALYRLGKIPELFSGLEELTPLPYANSPQAWSAAGAVELINLMEVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P926</accession>
    <name>A0A218P926_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_08090</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854529.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P926"/>
    <dbReference type="SMR" id="A0A218P926"/>
    <dbReference type="GeneID" id="33316223"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08090"/>
    <dbReference type="OrthoDB" id="24596at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005559">
        <property type="entry name" value="PRK07231.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF133">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="300" mass="32837" checksum="97A4C064E568D15A" modified="2017-09-27" version="1">MSGRFEGKTCIVTGGARGIGAAIAHRLASEGCKTAILDVDEEAGRVREKQLTSMGLSVKFFKADVSVEEEVSKAIEEVYETFGSIDVLVNNAGIGYGKPLELQTLDEWRRVIDVNLTGPYLCAKHAVRYMKERGGVIINIASTRALQSEPNTEPYSASKGGLLALTHALAMSLAPYRIRVLAVSPGWIDTSEWQIPPRKPELSPLDHGQHPAGRVGRPEDVAALVAFLASDEAGWMTGINVVIDGGMTKKMIYIDENVIEDSIKMLLQDTELASMIRTLIERAKKDRDEMREVLKELLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P930</accession>
    <name>A0A218P930_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1450 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_08165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854541.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P930"/>
    <dbReference type="GeneID" id="33316239"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08165"/>
    <dbReference type="OrthoDB" id="88303at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="68" mass="7467" checksum="EA02A95361C3310C" modified="2017-09-27" version="1">MEARVCKFCAGENLKDVVRALKERGFNVSVAECIGLCAKYECGNINVIAGRREISVKSLDEFIEALEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P960</accession>
    <name>A0A218P960_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Pyridine nucleotide-disulfide oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_08235</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854555.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P960"/>
    <dbReference type="GeneID" id="33316253"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08235"/>
    <dbReference type="OrthoDB" id="28009at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050260">
        <property type="entry name" value="FAD-bd_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054748">
        <property type="entry name" value="NROR-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429:SF3">
        <property type="entry name" value="NITRITE REDUCTASE [NAD(P)H]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22353">
        <property type="entry name" value="PF1197-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="4">
        <location>
            <begin position="1"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="NAD(P)H:rubredoxin oxidoreductase C-terminal" evidence="5">
        <location>
            <begin position="298"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="361" mass="39885" checksum="7F7293B18FDDB909" modified="2017-09-27" version="1">MKVVIVGNGPGGVELAKNLSGEFEVAVIEREELPHYSKPMLSHYIAGFLPEEKLFPLPSDWYEKRGIDLHLGTSAVLIDRAKRVLVTSKGAFPYDALVLATGARAREPAFQGKEHMLTLRTFGDARRIKEKLEEEGEMVILGGGFIALELAGNVAKAGYKVKLVHRKRNLLRLDEELSERIREELEGIGVEFHLETNVLSASENGLNTDRGHIPGRLKVCAFGIVPNKELAVRSGIHAGRGILIDDRFRTSAEDVYAIGDCAEYNGIICGTAKAATEHAKVLANLLRGVEDRYDFNFRSTVFKFADMNIALIGRTQGEGKWLDEETKVFYEDGKAVGAVVLGNVRRAFRLENAIREGLPID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P968</accession>
    <name>A0A218P968_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Archaeosortase family protein ArtF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_08300</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854568.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P968"/>
    <dbReference type="GeneID" id="33316266"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08300"/>
    <dbReference type="OrthoDB" id="100302at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027557">
        <property type="entry name" value="Archaeo_ArtF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026392">
        <property type="entry name" value="Exo/Archaeosortase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019127">
        <property type="entry name" value="Exosortase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04293">
        <property type="entry name" value="archaeo_artF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04178">
        <property type="entry name" value="exo_archaeo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09721">
        <property type="entry name" value="Exosortase_EpsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="169" mass="19086" checksum="1D9F48BB52DA659E" modified="2017-09-27" version="1">MKLSGERKNLLILAAYLTVFPIVTLLIGFAVGKPLIRIEASNIHLLLETFGIENHLVGGQIYVPADRMSFEITWQCSGAFSMTLYTLIYLLLPGLKKKPMEWLFGVSAIYIANILRVFFSIYLYYEFGEGAFNTFHYTVGPILLFLLVVILIAHVFLLGLRSSTKPSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P995</accession>
    <name>A0A218P995_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Rubrerythrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_08240</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854556.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P995"/>
    <dbReference type="GeneID" id="33316254"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08240"/>
    <dbReference type="OrthoDB" id="45654at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00729">
        <property type="entry name" value="rubredoxin_SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01041">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052753">
        <property type="entry name" value="Rbr2/Nigerythrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rr_diiron-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048574">
        <property type="entry name" value="RUBY_RBDX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33746">
        <property type="entry name" value="RUBRERYTHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33746:SF4">
        <property type="entry name" value="RUBRERYTHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21349">
        <property type="entry name" value="RUBY_RBDX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57802">
        <property type="entry name" value="Rubredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="4">
        <location>
            <begin position="2"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Rubredoxin-like" evidence="3">
        <location>
            <begin position="137"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="171" mass="19504" checksum="3C5185636CFE7DBF" modified="2017-09-27" version="1">MVVKRKMTRKFLEDAFAGESMAHMKYLIFAEQAEREGYPNIAKLFRAIAHAEFVHAKNHFIALGNLDKTPENLQAGIDGETFEVEEMYPVYKNTAEFQGEKDAVRTTHYALEAEKIHAELYKKAKEKAESGEDIEIKRVYICPVCGYTAVDEVPEYCPVCGAPRDKFVVFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9C2</accession>
    <name>A0A218P9C2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ADP-ribosylglycohydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08685</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9C2"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08685"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4080.10">
        <property type="entry name" value="ADP-ribosylation/Crystallin J1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050792">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005502">
        <property type="entry name" value="Ribosyl_crysJ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036705">
        <property type="entry name" value="Ribosyl_crysJ1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222">
        <property type="entry name" value="ADP-RIBOSYLGLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222:SF12">
        <property type="entry name" value="ADP-RIBOSYLGLYCOHYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03747">
        <property type="entry name" value="ADP_ribosyl_GH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101478">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR605502-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="381" mass="42728" checksum="8017FC6686E8A023" modified="2017-09-27" version="1">MPHSPNKVGRKLSECENTSCIMDVLFEEDLINAEQSKLLYSKPKLIDRVQLSKVRGMLLGVPIGDALGSPFEGRPPSEENFRMINDYLPEAHITDDTQLTFWTLEVFSERGWFDPKALADRFSSEWIIGIGGSVRRFIRNYKDLGKPWYLSGVESAGNGALMRLSPVVIPHLLNPTRELWSDAVITIYLTHNDRLAISSSVAFTNILWELFMKDKPPEPEWWVEEYVSVAREVEGDFSRYSPRFGKFKSYSGPAWEFVEKTLEKALSSGWSLLDLNTAVGSGAYLLETVPMVLYTMMLYADDPTKAITMAVSSSKDSDTVGAIVGYLVGALHGVKAFPEHLLAPVLNGNLMPRTFVDLVVKVETFLMNPAKGNVLNGHIWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9D6</accession>
    <name>A0A218P9D6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">5'-3' exonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854648.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9D6"/>
    <dbReference type="GeneID" id="33316358"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08760"/>
    <dbReference type="OrthoDB" id="33831at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018977">
        <property type="entry name" value="NurA_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09376">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00933">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NurA" evidence="1">
        <location>
            <begin position="45"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="416" mass="47639" checksum="CCC8E52487B3393F" modified="2017-09-27" version="1">MERISDVHVREIREFLLKSLRDVERLRDAVGRHFEWRPLPEPKEASIYAIDGSRMMKRLSGAIIYAVSASAIGERLYYWNDIGMVFPYKSVDERVRIHMDILEKRMGAMMLELGADLVLMDGTISSAIITPPTYVTSTTRELYERHGEQLLNAALEFLDFLDEQWVDWREKLEEGGVLNGFSLPSRGWGGEEIFSILMKRGAKSIRESFWWVNDREDLIVLFEYLEYLHALDRLLGGRIAAIAKTFYKSDVVKTVKAREGDKLKGTPMIVDTPVVASLSEEKGYLEFSYLKEPKGGLPRLIVDVMMRGKLQNLKEALILEGGKITGARIRPAYVRFADGGLIYLLEVPEKQDFERTLAEILSVAEDEYVIPLEYAHHSVVIKKQEFDAYVNAVLSALVGEDERFLSFLRYGREPLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9E6</accession>
    <name>A0A218P9E6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Large ribosomal subunit protein eL24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rpl24e</name>
        <name evidence="12" type="ORF">A3L08_08805</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9 10">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L14.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854656.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9E6"/>
    <dbReference type="GeneID" id="33316367"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08805"/>
    <dbReference type="OrthoDB" id="55506at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00472">
        <property type="entry name" value="Ribosomal_L24e_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.170.20:FF:000001">
        <property type="entry name" value="probable ribosome biogenesis protein RLP24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.20">
        <property type="entry name" value="Ribosomal protein L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00773">
        <property type="entry name" value="Ribosomal_eL24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038630">
        <property type="entry name" value="L24e/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056366">
        <property type="entry name" value="Ribosomal_eL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055345">
        <property type="entry name" value="Ribosomal_eL24-rel_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_eL24-rel_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023442">
        <property type="entry name" value="Ribosomal_eL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF034186">
        <property type="entry name" value="PRK14891.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01073">
        <property type="entry name" value="RIBOSOMAL_L24E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 10 12" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 10" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="5 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TRASH" evidence="11">
        <location>
            <begin position="7"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="10">
        <location>
            <begin position="7"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ07411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="67" mass="8035" checksum="CBC35DFF283D0A4D" modified="2017-09-27" version="1">MARWNVCSYCGKEFEPGTGKMFVRNDGRVMFFCSSKCEKLYFMGRNPRKLKWTKAFEEARLQRAKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9F2</accession>
    <name>A0A218P9F2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Prokaryotic stomatin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_08835</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854662.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9F2"/>
    <dbReference type="GeneID" id="33316373"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08835"/>
    <dbReference type="OrthoDB" id="10752at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08829">
        <property type="entry name" value="SPFH_paraslipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000004">
        <property type="entry name" value="Putative membrane protease family, stomatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050710">
        <property type="entry name" value="Band7/mec-2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018080">
        <property type="entry name" value="Band_7/stomatin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43327">
        <property type="entry name" value="STOMATIN-LIKE PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43327:SF10">
        <property type="entry name" value="STOMATIN-LIKE PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01270">
        <property type="entry name" value="BAND_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Band 7" evidence="9">
        <location>
            <begin position="21"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="284"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="284"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00080748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ07417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="308" mass="34643" checksum="28975A0EBFFBA639" modified="2017-09-27" version="1">MSFAAAALVIVGVFLLILLLLSVKVIRPYQKGLVERLGKFNRILEPGIHFIVPFMERVRVVDMREHVVDVPPQEVICKDNVVVTVDAVVYYQIMDPVKAVYNVSNFLMAIVKLAQTNLRAIIGEMELDETLSGRDIINARLREELDKITDRWGVKITRVEIQRIDPPKDIQEAMAKQMTAEREKRAMILLAEGKKESAIKEAEGQKQAAILKAEGEKQRQILVAEGQAEAIRKVLEALRMADEKYLTLQYIEKLPDLAKYGNLIVPYDTESLIGLLRILQKVKETPLPEPRPPEDGNSGENPAGAEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9G0</accession>
    <name>A0A218P9G0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Peptidase S9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_08890</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S9C family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854672.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9G0"/>
    <dbReference type="GeneID" id="33316384"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08890"/>
    <dbReference type="OrthoDB" id="25019at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000028">
        <property type="entry name" value="S9 family peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000199">
        <property type="entry name" value="Serine peptidase family S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011659">
        <property type="entry name" value="WD40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776:SF27">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE FAMILY MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776">
        <property type="entry name" value="SERINE PEPTIDASE S9 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07676">
        <property type="entry name" value="PD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="5">
        <location>
            <begin position="421"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="631" mass="72209" checksum="3186CDFB2BBDD2D8" modified="2017-09-27" version="1">MAKGLTEKDLGKFKLVGNIDAFKRKLVFQVTEISVEKDDYFSRLYLYDGRKVKPFTSGKKDGNPRFSPDGKLVAFTSKRDKESKEAELYVIPTDGGEARLLAKFKYGIRNLRFTEDGKSIAVVTPIDVEKKPKDDVHVIKEIPFWFNGVGWVYGRRSVVYLVDVETGRKKRLTPKNLDVSQIRFHNGRFYFTAQEDRERKPMVSDLYVLEGRKAKRLTPGKWSVSDFIPLDDGTFILKANTRERGIPTNTHIYHYDPQTGEMRKLTKNLDRSAYNSLNCDVRGSQRAELVFKDGWVYYVATDGPRANLFRVNLDGKIERVVGGDRSVESFAIGDYIAFTAQDAVTPTELYVMRDGKEKRVTDFNGWIKDYNLSQPEHFKVKASDGAEIDAWVMRPLGFEPGKKYPAVLEIHGGPKTAYGYSFMHEFHVLTAKGFVVIFSNPRGSDGYGEEFADIRGHYGERDYQDLMEVVDEALKRFDFIDPERIGVTGGSYGGFMTNWIVGHTNRFKAAVTQRSISNWTSFFGTTDIGYYFAPDQIGGDPWSNTEGYWEKSPLKYAPNVETPLLIIHSMEDYRCWLPEALQFFTALKYLGKTVELALFPGENHDLSRGGKPKHRVRRLELIAGWMERWLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9G4</accession>
    <name>A0A218P9G4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Acetylornithine deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_08645</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854627.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9G4"/>
    <dbReference type="GeneID" id="33316335"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08645"/>
    <dbReference type="OrthoDB" id="24854at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050072">
        <property type="entry name" value="Peptidase_M20A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006403">
        <property type="entry name" value="PRK08652.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808">
        <property type="entry name" value="ACETYLORNITHINE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808:SF25">
        <property type="entry name" value="PEPTIDASE M20 DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00759">
        <property type="entry name" value="ARGE_DAPE_CPG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="5">
        <location>
            <begin position="152"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="350" mass="39529" checksum="BB5E611F15328033" modified="2017-09-27" version="1">MKTERAKEILLQLLRIPSPSGREDRIMLHIMEFLHRLDYDVHIESDGEIIDLVVNPDADLFYEVHVDTIPIRAEPFVRGNIIYGTGSSDIKGGAAAILLMMESLKKEGKDLNVGVVFVSDEELGGRGSALFMERYRPKMAVVLEPTDLEVHIAHAGNIEAYFEVDGKEAHGACPESGVNAIEETYKMLEEMKKLEPFKQKGKYFDPHIGIQELVCENPVYLIPALCKGRLEARLLPDQEVEDILDLLDPILDEYTLKYEYTEIWDGYELDESEEIVQLAKKAMEKTEVDEFGGMRSWTDAINFMYNGTKTIVFGPGNLDISHTKNEHIDVRDVVTASEFLKVLNDIYGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9H3</accession>
    <name>A0A218P9H3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Amidohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_08845</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854664.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9H3"/>
    <dbReference type="GeneID" id="33316375"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08845"/>
    <dbReference type="OrthoDB" id="372084at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01298">
        <property type="entry name" value="ATZ_TRZ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050287">
        <property type="entry name" value="MTA/SAH_deaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794:SF11">
        <property type="entry name" value="AMIDOHYDROLASE-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794">
        <property type="entry name" value="AMINOHYDROLASE SSNA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="2">
        <location>
            <begin position="52"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="421" mass="46761" checksum="5D0ED9F585A6D600" modified="2017-09-27" version="1">MIALTGTLVDAQRTLRDVAVIVESGIIRAVVPADELREYGVDKVYGGRGYLILPGLVNAHTHVAMARFRGLGEDVPIERWLNEIIWPAELEWDPKEIRRWALLGMAEALANGSTTINDHYFFANEIAEVAQKLGIRAFIGQTIMDEVDFPIASPEEGFRFFKRWDGKDDLVRPTLAPHATNTVSLELMKEIGEFARERDALIHVHLSQSRGEVETVKRRYGLTPVEYLKRAGVLNERLIGVHGIYLGDSEVELYSRSGATLVHCSLSMAKLEGRIAPIIELAERGTNIALGNDSPNPVGLMDMFEEMRFAAVLNKVWRKRTDVASAREVFRWATVGGAKALGLRAGLVKPGYLADLILIDARKPQFLPGENPYSHVVYSARGSDVEMVMVNGEVVYRNGLLTKIGKTLGELWGETEKNLAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9H6</accession>
    <name>A0A218P9H6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Nodulation efficiency protein D-like protein (NefD)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_08840</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854663.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9H6"/>
    <dbReference type="GeneID" id="33316374"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08840"/>
    <dbReference type="OrthoDB" id="86084at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052165">
        <property type="entry name" value="Membrane_assoc_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002810">
        <property type="entry name" value="NfeD-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33507:SF7">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33507">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YBBJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01957">
        <property type="entry name" value="NfeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141322">
        <property type="entry name" value="NfeD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="NfeD-like C-terminal" evidence="6">
        <location>
            <begin position="86"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="141" mass="15603" checksum="C06BD66D8446F791" modified="2017-09-27" version="1">MDPLPISLLILGLLIIVLDMMVAAFITPIGVAFATLGILLGFGIEFTESFVLSLIAAVLAYFAVGRYIRRDVQDIGAREKKYTFELKGKRGKVVEIGKEHYIVELEGDKWIALPEGDEKLKIDDVVEVVDVDGVKLIVRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9K8</accession>
    <name>A0A218P9K8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Allophanate hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_09115</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854711.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9K8"/>
    <dbReference type="GeneID" id="33316429"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09115"/>
    <dbReference type="OrthoDB" id="84558at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003833">
        <property type="entry name" value="CT_C_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010016">
        <property type="entry name" value="PxpB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00370">
        <property type="entry name" value="5-oxoprolinase subunit PxpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34698">
        <property type="entry name" value="5-OXOPROLINASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34698:SF2">
        <property type="entry name" value="5-OXOPROLINASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02682">
        <property type="entry name" value="CT_C_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00796">
        <property type="entry name" value="AHS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160467">
        <property type="entry name" value="PH0987 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxyltransferase" evidence="4">
        <location>
            <begin position="3"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="231" mass="25565" checksum="C2537F36BADC12E5" modified="2017-09-27" version="1">MQPVINPAGDSTLLISFGETIDEGINERVHAIARAIEKAGFEWLVETVPAYSSLLVIYDPLKVTYTEVEASIKPLLNAEGCTVEGRLVKIPVVYGGKYGPDIEFVAEHNGLTVDDVIEIHSRPVYRVYFLGFLPGFAYLGGMDERIATPRLEKPRLKVPAGSVGIAGKQTGIYPLESPGGWRLIGRTPLRLFNPAKEPPTLLQPGDRVKFVPIDEEEFRELYEKEWGEGVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9L3</accession>
    <name>A0A218P9L3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Endoribonuclease Nob1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">A3L08_09125</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Toxic component of a type II toxin-antitoxin (TA) system. Processes pre-16S-rRNA at its 3' end (the D-site) to yield the mature 3' end.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NOB1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854713.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9L3"/>
    <dbReference type="GeneID" id="33316431"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09125"/>
    <dbReference type="OrthoDB" id="27944at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09876">
        <property type="entry name" value="PIN_Nob1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000020">
        <property type="entry name" value="20S-pre-rRNA D-site endonuclease NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000024">
        <property type="entry name" value="rRNA maturation endonuclease Nob1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039907">
        <property type="entry name" value="NOB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033411">
        <property type="entry name" value="Ribonuclease_PIN"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009146">
        <property type="entry name" value="PRK12496.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814:SF2">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17146">
        <property type="entry name" value="PIN_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="6" id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="domain" description="PIN" evidence="13">
        <location>
            <begin position="6"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ07469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="166" mass="18743" checksum="4DE33392C5DE137B" modified="2017-09-27" version="1">MREDGKVRVIDAAIFIQGVDVEGVTTPKVVEEVKDPESRLFLESLISAGKVRVLVPSKESIEAVKDAARRTGELNELSEADLEILALAYELNGVLFTDDYNLQNIARTLGTEFKTLKRGIKRVIRWNYVCIGCGRKFEEMPPDGVCPDCGSPVRLIPRKKRRQRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9P6</accession>
    <name>A0A218P9P6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Maleate cis-trans isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09270</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854736.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9P6"/>
    <dbReference type="GeneID" id="33316460"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09270"/>
    <dbReference type="OrthoDB" id="41425at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053714">
        <property type="entry name" value="Iso_Racemase_Enz_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026286">
        <property type="entry name" value="MaiA/AMDase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40267">
        <property type="entry name" value="BLR3294 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40267:SF1">
        <property type="entry name" value="BLR3294 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17645">
        <property type="entry name" value="Amdase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015736">
        <property type="entry name" value="MI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="237" mass="26482" checksum="6269DD3700CC0753" modified="2017-09-27" version="1">MYGWRGRLGLIVPSSNTTMEMELHNYLPEGLSLHTARVPLRNVNEEELIKMNSLAVEAAKLLGDAGVELILYGCTSGSFIGGKDYEKELEARIESEANVPVVSTSTAVVEALKILDAQSILVITPYTDEINRREKEFLEANEFEVLDIRGLGIEDNAQIGKLEPYEAYRLAKASFIDEADAIFISCTNLRTFEIIEALEEDLGVPVVTSNQASFWLALREMDVMEKIPELGRLFTEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Q5</accession>
    <name>A0A218P9Q5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854714.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Q5"/>
    <dbReference type="GeneID" id="33316433"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09135"/>
    <dbReference type="OrthoDB" id="86145at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="49"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="131" mass="15277" checksum="5367A3C2C74A6F57" modified="2017-09-27" version="1">MKLIIRPEKGLGKIEVELDDELTGEINRLSERYGVPAERVIEIALTGEFRESAGEIEELENKVKELEERTWELEREYAPLRFKAYGISEDNKILAIELSGLIAENNQLRRFLRLPLKGNPELRKLISYYMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9R7</accession>
    <name>A0A218P9R7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Transcription initiation factor IIB</fullName>
            <shortName evidence="10">TFIIB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 13" type="primary">tfb</name>
        <name evidence="13" type="ORF">A3L08_09455</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854771.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9R7"/>
    <dbReference type="GeneID" id="33316497"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09455"/>
    <dbReference type="OrthoDB" id="7429at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20549">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20550">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000023">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.170:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00383">
        <property type="entry name" value="TF2B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023484">
        <property type="entry name" value="TFIIB_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001658">
        <property type="entry name" value="PRK00423.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="12">
        <location>
            <begin position="3"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="repeat" description="1" evidence="10">
        <location>
            <begin position="114"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="repeat" description="2" evidence="10">
        <location>
            <begin position="210"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00053882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ07527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="299" mass="33949" checksum="7A3F6ADDD16A0B4F" modified="2017-09-27" version="1">MSNRRVCPVCGSTEFIYDPGRGEIICKVCGYVIEENVVDMGPEWRAFDASQREKRARVGAPESILLHDKGLSTDIGIDRNLSGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRIASQLSLPRHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEIADISRVDKKEIGRSFRFIARNLNLTPKKLFVKPTDYVNKFADELNLSEKVRRRAIAILEEAYERGLTSGKSPAGLVAAALYIASLIEDEKRTQREVAEVARVTEVTVRNRYKELVDKLNLKIPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9U3</accession>
    <name>A0A218P9U3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09615</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9U3"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09615"/>
    <dbReference type="OrthoDB" id="11691at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="147" mass="16606" checksum="F73D60DF327C2A85" modified="2017-09-27" version="1">MLDVGSGSGYPALLIALSGNATEVVGVEPDRHIFEFLKRLEKLAENLRFINGDFLDRGLELEGFDTVVFSYILHDFDPLPFIERALDVLKPGGRIIVGDFDLNGLRETLKRFVREKRVKILENLTIGEAPTHRQRAKAFIIVMEVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9U6</accession>
    <name>A0A218P9U6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_09360</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the PINc/VapC protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854754.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9U6"/>
    <dbReference type="GeneID" id="33316478"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09360"/>
    <dbReference type="OrthoDB" id="7146at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09878">
        <property type="entry name" value="PIN_VapC_VirB11L-ATPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052041">
        <property type="entry name" value="Nucleic_acid_metab_PIN/TRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010335">
        <property type="entry name" value="PRK13764.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11603">
        <property type="entry name" value="AAA FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11603:SF147">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="2">
        <location>
            <begin position="2"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00046345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="602" mass="68154" checksum="85CE81293A62C062" modified="2017-09-27" version="1">MKVFVADTSVIVDGRLTQFLSTLNEKVKVVIPEAVVAEIEHQANEGKAIGHVGLEELKKLRKMADEDRILLEFHGDRPELWQIRRAKAGEIDHMVREAARELGAILITCDQVQRDVAIAKGIEVIYLTAKKEIRHRLEDFFDETTMSVHLKGGLRPFAKKGRPGDWKLVPVRDEVLTDEELYEIADDIVERARRDPESFIEMDEPGATVVQLRNYRIVIAKPPFADRIEITAVRPVKKLSIEDYELSEKLLERLKDRAQGILIAGAPGEGKTTFAQALAEWYASMGRIVKTMEKPRDLQVSEEITQYTALNGRMELTGDILLLVRPDYTIFDEMRKTSDFKIYADLRLAGVGMVGVVHATKPIDAIQRFIGRVELGMIPQIVDTVIFIKAGRVEKVLTLEYLVKVPSGMKEEDLARPVIEVRDFETGELEYEIYTYGEEVSVVPVKKEEKAPALKLAERRLKQEIKKFLPDVYTEVEIVSPHKAVIYADEFDIPAIIGKKGKRITDLEKKIGISIDVKSFTEREAEKPKEKIPVEVEEKKKTIVLRVSPDYAKKPLKFYGGEQYVFTATPSKKGLVKVSKSTPIGKELKRLIDAGIPIWATT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9V0</accession>
    <name>A0A218P9V0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_232461730.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9V0"/>
    <dbReference type="GeneID" id="33316524"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09590"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="288" mass="32056" checksum="6A72FFC0D51E19FF" modified="2017-09-27" version="1">MRRYFLSSVFIVLLVVGFLVWHGKSTPKPSLEYPLNSSKLRSFLESQYVPEANLLRAAVKAYPDNETIYIANDNLLASRALVALGSPIGRRILENLNANYSGGWSGKVDPLPGRPIAGFYCVETRNLGKVYSKKFNATFEIKYEIANTSCRMEDWESYADLLVYGALNELINGNESGALEIYSRLLGMWDGNGFRDRAFDGTYQTYKCALFVYLYRALGKPKEGGNVYSHCLNVISSLQAENGGIITGYWVENGKIAPVGDTNTETTSMVVLAVYSGYPTTIKEVYQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9V8</accession>
    <name>A0A218P9V8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GNAT family acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09655</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854808.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9V8"/>
    <dbReference type="GeneID" id="33316537"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09655"/>
    <dbReference type="OrthoDB" id="134118at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43072:SF60">
        <property type="entry name" value="L-2,4-DIAMINOBUTYRIC ACID ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43072">
        <property type="entry name" value="N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="278" mass="31835" checksum="03DF59383320BC91" modified="2017-09-27" version="1">MNPIIREARPEDRPFIKEIARLTWGGEDYLARVFDDWVKDGNFYVLELEGKVIGTAKLTLLPGKVGWLEGLRVHPDYRGRGYGRMIQNFMIDLGRRLAEQGKIEALEFATYFLNRESIAMARKDGFSVMARFFNLGARVEDFQPEKPNPVELGMNDLTLGIIPLGWKFVHRSEEALEWLREKGEAYEVNDFKFLATRDGATFTPLSTGLGCIRAMLPAMAWVAREKGREEFDLMLPGGMKPVLPGLRRLGLFLWDETDEPNVLVFRKKLPERSVIKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9X5</accession>
    <name>A0A218P9X5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">tRNA-Thr(GGU) m(6)t(6)A37 methyltransferase TsaA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_09775</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the tRNA methyltransferase O family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854830.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9X5"/>
    <dbReference type="GeneID" id="33316561"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09775"/>
    <dbReference type="OrthoDB" id="40408at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09281">
        <property type="entry name" value="UPF0066"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.70">
        <property type="entry name" value="YaeB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023370">
        <property type="entry name" value="TrmO-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023368">
        <property type="entry name" value="UPF0066_cons_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040372">
        <property type="entry name" value="YaeB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036413">
        <property type="entry name" value="YaeB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036414">
        <property type="entry name" value="YaeB_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00104">
        <property type="entry name" value="tRNA_TsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12818">
        <property type="entry name" value="TRNA (ADENINE(37)-N6)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12818:SF0">
        <property type="entry name" value="TRNA (ADENINE(37)-N6)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01980">
        <property type="entry name" value="TrmO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118196">
        <property type="entry name" value="YaeB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01318">
        <property type="entry name" value="TSAA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51668">
        <property type="entry name" value="TSAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TsaA-like" evidence="3">
        <location>
            <begin position="8"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00033753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="221" mass="25774" checksum="F289CAB4AAA11B5E" modified="2017-09-27" version="1">MEFEPFKIVPVGYVRKNEELQETFIEIFPEFREAMEGLREGDWVKLILWFHGSDTPERRRVLKVHPHGNPKNPLRGVFATRSPYRPNPVALYTVRVHRIAGNRLYIDWIDAMDETPVVDIKIFVERYDCPNEVTIEEREAHIRGGRMIGEVNVIPRKSEHLDELEEVSPEEYDALILEIGPKTTTLTAEELVELIDAMREAYDALPVEIKDRLRTREGRSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Y5</accession>
    <name>A0A218P9Y5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_00015</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854841.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Y5"/>
    <dbReference type="GeneID" id="33314606"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00015"/>
    <dbReference type="OrthoDB" id="7080at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016423">
        <property type="term" value="F:tRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RlmKL-like_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14911">
        <property type="entry name" value="THUMP DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14911:SF13">
        <property type="entry name" value="TRNA (GUANINE(6)-N2)-METHYLTRANSFERASE THUMP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA large subunit methyltransferase K/L-like methyltransferase" evidence="2">
        <location>
            <begin position="175"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="366" mass="42774" checksum="61FF0C000194AEE5" modified="2017-09-27" version="1">MYAVLFGKNPALSEAEFYSFSRRFGLKVRPIESSRYWLLFDSSPDVERQFYWLGGSLKLVRIIGEGEEAIRDLEYSKLFTVSLYGRSDWKLWRKLGSEIKRHFKEEAPAKFFKPAKVYSMPAELILKGFPEVKDFVFLFREDGSFLVGETVKVTDPFELKKLDVERPVQKPILSIPPRLARIMVNLTEVRKGSFLDPFCGIGTVVQEFVLQGLNAYGSDRDPEQIKAAKKNLAWLRKEFRLKNSAHLEVCDARKLKRCFRQRFDAIVTEPYLGKPLRRNPSRGEAIKLANELDRFYYSVFESFADVLKRNGKVVFVFPAYKLSDGGIYRKERKWLGKLGFEVLGRYTDYEERHRLVRDIHVLRYRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Z7</accession>
    <name>A0A218P9Z7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">3-phosphoglycerate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_01245</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854854.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Z7"/>
    <dbReference type="GeneID" id="33314853"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01245"/>
    <dbReference type="OrthoDB" id="7437at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12173">
        <property type="entry name" value="PGDH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000203">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase (SerA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050857">
        <property type="entry name" value="D-2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42789">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G10090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42789:SF1">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G10090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00670">
        <property type="entry name" value="D_2_HYDROXYACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="6">
        <location>
            <begin position="3"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="7">
        <location>
            <begin position="105"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="304" mass="33137" checksum="5E78F9A361721004" modified="2017-09-27" version="1">MKVLVAAPLHEKAIEVLKNAGFEVVFEEYPDEERLIELVGDVDAIIVRSKPKVTRKVIEAAPKLKVIGRAGVGLDNIDIEAAKERGIKVVNSPGASSRSVAELAVALMFNVARKVAFADRKMREGVWAKKQCMGVELEGKTLGVVGFGRIGYEVAKIAHALGMNVLLYDPYPKEERAKEVGGKFVDLETLLKESDVVTLHVPLIDATYHLINEERLKLMKPTAILINAARGAVVDTNALIKALQEGWIAGAGLDVFEEEPLPKDHPLTKFDNVVLTPHIGASTVEAQMRAGVQVAEQIVEILKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA19</accession>
    <name>A0A218PA19_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amidohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_04445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA19"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04445"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01300">
        <property type="entry name" value="YtcJ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013108">
        <property type="entry name" value="Amidohydro_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033932">
        <property type="entry name" value="YtcJ-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22642">
        <property type="entry name" value="IMIDAZOLONEPROPIONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22642:SF2">
        <property type="entry name" value="PROTEIN LONG AFTER FAR-RED 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07969">
        <property type="entry name" value="Amidohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase 3" evidence="1">
        <location>
            <begin position="54"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="536" mass="59569" checksum="28FD3EFA8FF48B42" modified="2017-09-27" version="1">MLMVVLVKAFVNGRIYVSFKPLKTVDAILVASGRIVYAGSSEMAIKIARELGGEVVDLEGRVVLPGFIDSHLHLEELGMYLETLDLRGVGSIAELKERVRSYAEIAKTSWILGHGWDQELFEENRWPMRWDIDEVVDDRPVMLSRVCLHAAVLNTEAMELAGLLDSELPGVMRDENGEVTGVVKEEAFELAREKFKETLTLEDYEHFVKMAVDYAASLGITAVGTVSVEEKTLKAISNLEERGELKIRVFAYIDPGKREVKGNGMFGNLDVLDSLKKLGIRRGFGRGKLRINGIKVLADGSLGARTAWLSEPYSDAPTQGYANISRELLEKIVRGAHEAGLQMAVHGIGDATIDMILDVYSSLDDPGKLRHRIEHASILRPDQIERMARLGVVGAVQPHFVVTDWWAVRRVGKERAGWVYPFRSMLDAGIVLGFGTDSPIEPTNPWETVYAAVTRGKYEGAEPYEYTKNEALSLEEALHAYTYGSAYILGAEDELGTLEEGKFADFVVVDKDPFGMDERKLREVKVLETYVGGEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA47</accession>
    <name>A0A218PA47_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">16S rRNA methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_04205</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854886.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA47"/>
    <dbReference type="GeneID" id="33315445"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04205"/>
    <dbReference type="OrthoDB" id="4668at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046977">
        <property type="entry name" value="RsmC/RlmG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47816">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47816:SF4">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase small" evidence="3">
        <location>
            <begin position="28"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="195" mass="21817" checksum="1AB6F9E094068C7B" modified="2017-09-27" version="1">MSHYYSEEPNTPLKTKTIEVCIRGHCFKFITASGVFSFGKLDRGTELLIESMVLDEKWRALDLGCGYGAIGIVASRFVDYVVMTDVNRRALNLARKNLKINSVRNAEVRWGSLYEPVKGEKFDTIITNPPVHAGKDILREIVINAPQHLNDGGLLQLVIKTKQGAKYIKALMDDHFTEVRELAKGSGYRVYAGIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA51</accession>
    <name>A0A218PA51_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sodium:solute symporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06925</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854910.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA51"/>
    <dbReference type="GeneID" id="33315988"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06925"/>
    <dbReference type="OrthoDB" id="102144at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="62" mass="6595" checksum="FCD747A2EE95F631" modified="2017-09-27" version="1">MDVMWTTIAVYMGAMLAVSAYARKYIKTSADFLLAGRRLGLGLTVGTLAATHYGGGFHPGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA74</accession>
    <name>A0A218PA74_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_08435</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA74"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08435"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058303">
        <property type="entry name" value="DUF7990"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25952">
        <property type="entry name" value="DUF7990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="89" mass="10285" checksum="D9CDC2196FF46D2F" modified="2017-09-27" version="1">MLESIQDFLKGIEQSFREQSTEYVEFELRELENVFALLLMGSFVGLPSPPTTLTMRLLPHMARELYVMQRRAEDMDDILGELAGLFDIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P4X3</accession>
    <name>A0A218P4X3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CoA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853094.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P4X3"/>
    <dbReference type="GeneID" id="33314609"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00030"/>
    <dbReference type="OrthoDB" id="42776at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33303:SF2">
        <property type="entry name" value="COA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33303">
        <property type="entry name" value="CYTOPLASMIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA-binding" evidence="1">
        <location>
            <begin position="18"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="142" mass="16626" checksum="E554EBB3E5337A63" modified="2017-09-27" version="1">MVRIMPVDRLSDEEIMEILTKYRKIALVGASPKPERDANRVMRYLLEKGYEVYPVNPRYEEVLGRKCYPSVLDIPDEVDIVDLFVRPEFTMEYVEQAIEKGAKVVWFQFNTYNRDAFKKAKEAGLTAVAHRCIKQEHERLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P4Z2</accession>
    <name>A0A218P4Z2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoesterase</fullName>
            <ecNumber evidence="1">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_00090</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="1"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853106.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P4Z2"/>
    <dbReference type="GeneID" id="33314622"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00090"/>
    <dbReference type="OrthoDB" id="19174at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00841">
        <property type="entry name" value="MPP_YfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041802">
        <property type="entry name" value="MPP_YfcE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11124">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN VPS29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU362039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ05843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="164" mass="18329" checksum="51735E325324B6A0" modified="2017-09-27" version="1">MIIAVTSDTHHGDKTNNLPSLLLKELEKQRPDLILHAGDVTSAELLETLEEFAPTIAVRGNADHLQLPEEQVLDAEGVRIGLLHGHRFFSLNAQFLTLKALDMGVDVLIFGHTHRFYHDTYSVHGQRVILLNPGSPTFPRMDSAGFALLKINGESVRVERVTFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P4Z7</accession>
    <name>A0A218P4Z7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">CopG family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_00165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853121.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P4Z7"/>
    <dbReference type="GeneID" id="33314637"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00165"/>
    <dbReference type="OrthoDB" id="101495at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ05858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="112" mass="13238" checksum="1219B53AA39AA6EF" modified="2017-09-27" version="1">MSKDKIPKLFEGSVNELTKPSKPKKEEKALKSKDLKKEKMQKTLYISRDMNKKLIELYADEGRRQSAIVEDAVNLYYYLKLALGEKKFNELLSAVKREDPDFLRDYMEKFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P547</accession>
    <name>A0A218P547_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Sodium:proline symporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">A3L08_00455</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-proline(in) + Na(+)(in) = L-proline(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28967"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853175.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P547"/>
    <dbReference type="GeneID" id="33314695"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00455"/>
    <dbReference type="OrthoDB" id="9779at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005298">
        <property type="term" value="F:proline:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031402">
        <property type="term" value="F:sodium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015824">
        <property type="term" value="P:proline transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11475">
        <property type="entry name" value="SLC5sbd_PutP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011851">
        <property type="entry name" value="Na/Pro_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050277">
        <property type="entry name" value="Sodium:Solute_Symporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48086">
        <property type="entry name" value="SODIUM/PROLINE SYMPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48086:SF3">
        <property type="entry name" value="SODIUM_PROLINE SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="11" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="67"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="230"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="278"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="324"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="408"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="434"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="458"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ASJ05912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="488" mass="52357" checksum="7AC65F727B29E152" modified="2017-09-27" version="1">MENQTQILTVLVLYLSFLIGFGVYQGRKAKSGKDFSIAGRQLPGWVAALSERATGESAWALLGLPGFAYAAGLSAIWVAIGCVAGIIVAWTVFAGRLRREAEKYDATTFIDYIAKRHADSGKWIRILGSFTVVFFFFFYVGAQFLGGGKTLNALFGIDPKVGMLITALIILPYTVLGGLKSVAYTDTVQAIVMILTLTIAPIVGLAYIASHPDVFAHSVTSALEMSGPEYSSILGGLAGGAALVFVIAEFSWFFGYLGGMPQLSIRFMAIKDEKNAKLARNVGVGWTILAYIGALLIGWIGIAIFGPGGLSDQEQVMPSVMLKLFPPFLAAVFITGAIAAMLSTADSLLILSATELSENLLKPFVYKDDFDPKRSLRLSRISTVALGVIALITAYVVPSNLIYTIVGYTWAGIGDTFSVIVILTLFWKKFHGRAVPPTIVTGLLFTIFWISSGLEEIVSARLMTFIVTAVVAVISTYALKPKEAASTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P562</accession>
    <name>A0A218P562_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Indolepyruvate ferredoxin oxidoreductase subunit beta</fullName>
            <ecNumber evidence="5">1.2.7.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_00470</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ferredoxin-dependent oxidative decarboxylation of arylpyruvates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>indole-3-pyruvate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = (indol-3-yl)acetyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12645"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17640"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57271"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="EC" id="1.2.7.8"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer of the IorA and IorB subunits.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.8" evidence="5"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853178.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P562"/>
    <dbReference type="GeneID" id="33314698"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00470"/>
    <dbReference type="OrthoDB" id="53326at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043805">
        <property type="term" value="F:indolepyruvate ferredoxin oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017719">
        <property type="entry name" value="Indolepyruvate_Fd_OxRdtase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052198">
        <property type="entry name" value="IorB_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03334">
        <property type="entry name" value="IOR_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005326">
        <property type="entry name" value="PRK06853.1-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43854">
        <property type="entry name" value="INDOLEPYRUVATE OXIDOREDUCTASE SUBUNIT IORB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43854:SF1">
        <property type="entry name" value="INDOLEPYRUVATE OXIDOREDUCTASE SUBUNIT IORB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate/ketoisovalerate oxidoreductase catalytic" evidence="7">
        <location>
            <begin position="12"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="NCBIfam" id="TIGR03334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ05915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="202" mass="22063" checksum="3ADEE5221767C477" modified="2017-09-27" version="1">MKEYNIVITGVGGQGVLTAANILGWAALHAGHKVRMGEVHGMSQRFGSVVSYVRFGEEVYGSMVPEGKGDVILAFEPVEALRYINYLKEGGMVVVNTKPIVPVQVSMGKARYPELEEIKRIVEEDFKGKWIGFNAEELAMKAGHVVTTNTVLIGALTQVPEFPLDAEHVREVIRLSVPPKAVDMNMKAFDLGIEAAKKILGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P565</accession>
    <name>A0A218P565_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UPF0056 membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_00575</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the UPF0056 (MarC) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P565"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00575"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002771">
        <property type="entry name" value="Multi_antbiot-R_MarC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00427">
        <property type="entry name" value="NAAT family transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33508">
        <property type="entry name" value="UPF0056 MEMBRANE PROTEIN YHCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33508:SF1">
        <property type="entry name" value="UPF0056 MEMBRANE PROTEIN YHCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01914">
        <property type="entry name" value="MarC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ05934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="213" mass="23161" checksum="B7EF4AF6E6F45D9A" modified="2017-09-27" version="1">MIEYLKYFIILYGGLFAITNPVGAVPVFLSVTHDLSWRERREIATKTAIAVVVTLVVFALIGEWIFKFFGSSTDAFAIAGGILLFKMAMEMLSGKLSTVKISTEETEEFSEEVVTLEEVAIIPLAIPLISGPGAITTVMLYMAKSHHLSEKATVIASIVAIGLTVWLVLCSSNRIQKKLGRVGIKVMTRMMGLILTSMAVQMIINGIKGAFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P595</accession>
    <name>A0A218P595_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">dCMP deaminase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_00810</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853236.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P595"/>
    <dbReference type="GeneID" id="33314766"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00810"/>
    <dbReference type="OrthoDB" id="7284at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004132">
        <property type="term" value="F:dCMP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006220">
        <property type="term" value="P:pyrimidine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01286">
        <property type="entry name" value="deoxycytidylate_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016473">
        <property type="entry name" value="dCMP_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015517">
        <property type="entry name" value="dCMP_deaminase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035105">
        <property type="entry name" value="Deoxycytidylate_deaminase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11086:SF18">
        <property type="entry name" value="DEOXYCYTIDYLATE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11086">
        <property type="entry name" value="DEOXYCYTIDYLATE DEAMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006019">
        <property type="entry name" value="dCMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="6">
        <location>
            <begin position="21"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ05974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="178" mass="20188" checksum="FB3FEBBAB526230B" modified="2017-09-27" version="1">MNVEIFLDREKAERIKRIRPTKDEYFMLIAKLVSLRATCPRLRVGAVAVKDGYILATGYNGAPRGMEHCIDVGCLIVDGHCHRAVHAEQNVIAMAARKGISLEGATLYVTHFPCDTCFKIVLNAGIKEIVYEEMYPNEATEILLEEAQKKGIVKIRQFKLSKERVRLFLEELFGEFVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P598</accession>
    <name>A0A218P598_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Sodium:proline symporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">A3L08_00630</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-proline(in) + Na(+)(in) = L-proline(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28967"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853207.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P598"/>
    <dbReference type="GeneID" id="33314730"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00630"/>
    <dbReference type="OrthoDB" id="9779at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005298">
        <property type="term" value="F:proline:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031402">
        <property type="term" value="F:sodium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015824">
        <property type="term" value="P:proline transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11475">
        <property type="entry name" value="SLC5sbd_PutP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011851">
        <property type="entry name" value="Na/Pro_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050277">
        <property type="entry name" value="Sodium:Solute_Symporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48086">
        <property type="entry name" value="SODIUM/PROLINE SYMPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48086:SF3">
        <property type="entry name" value="SODIUM_PROLINE SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="11" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="5"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="227"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="272"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="323"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="372"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="395"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="432"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="471"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ASJ05945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="538" mass="58461" checksum="CAE31CCB5DBC8A61" modified="2017-09-27" version="1">MNAGVLFGFLVYLALLAYIGWWANKYTKTEDQYFVGGRKVHVLAATLSDKASDFSGWLMLGYPGSAFKAGLGAFWAGIGCLFGTLADYVLIGPRLRIYAGKFRAITVPDYLEARLKDNTKLIRILSALIIIIFMTAYVAAQFTAGGKTFAEGFGVSVNTGILVTVIILTAYVITGGFFAVVWTDVVQALFMLLTLIIVPFLALAKIGGLDKATDIIASADPTKLHPFGGATGWAAIIFAIGYASWVVGYLGQPHIVTRYMSVEDPRKLRRPGIFISGIWTTIVLWGAFFAGFLGFALYQAGMLNVSDPERVIPAMAVELMPSWIAGFVIAGIISAVMSTADSQLLVASSAIARDFYHKVLGKEVGKKQMVNISRLVVFGVALVGLWFAISGPKVIYQMVATAWGGLAVGFGPILTLSLWWKRVTKEGGIVGMAYGLVSEVIFEAKIYGWAFTKDAPGIWGTIGGWFQDVPVFFINFFITLFIIIIVSLLTKPPEDVVKLHEELFRKVPIETGRKSITETRAKSQVENVAEFIIAKGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5A6</accession>
    <name>A0A218P5A6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Zinc metallopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">A3L08_00805</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the peptidase M48 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5A6"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00805"/>
    <dbReference type="OrthoDB" id="28389at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050083">
        <property type="entry name" value="HtpX_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43221">
        <property type="entry name" value="PROTEASE HTPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43221:SF2">
        <property type="entry name" value="PROTEASE HTPX HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 10" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M48" evidence="11">
        <location>
            <begin position="57"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ05973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="260" mass="29292" checksum="76215B1B295535D1" modified="2017-09-27" version="1">MLFIIACLEVLLAVIALAELGLKISLAALGAIALLYLWASTRDVGGNYTPLQRIEMPWLYDGIAEMARKAGLPMPRVYLLDDYIPTAFSFKNTIVLSLGLFEVLDPDEILAVAAHELGHIKSGDTKTFPILACGRYLMMGLTAVLIVLTRNAPMSMAALGLMGLYEVTRANFHKEREFRADETALRLLETPLSLKRALEELKYYEDLRAGIKHRALPSIEPTIERKQRTQIIETHPSYDERVLRILIEINRGNMLNHRME</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5B0</accession>
    <name>A0A218P5B0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_00725</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853221.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5B0"/>
    <dbReference type="GeneID" id="33314749"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00725"/>
    <dbReference type="OrthoDB" id="85793at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012096">
        <property type="entry name" value="ATPase_PP-loop_MJ1638"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052188">
        <property type="entry name" value="Ni-pincer_cofactor_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43169:SF1">
        <property type="entry name" value="ATPASE, PP-LOOP SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43169">
        <property type="entry name" value="EXSB FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006601">
        <property type="entry name" value="ATPase_UCP006601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ05959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="246" mass="27651" checksum="29BD68A10A299252" modified="2017-09-27" version="1">MIKPVRDDFVKGYRLQYNLEALERVRGEIGEEAYSRLKALIEYRLYRKDFDRSPTGVKIALAFSAGSDSTASLKILRWAGFDVVPVTAKLPQMNEHVIEKARREGAVLVEVPNYLEVITSQMEKGAPICGRCHSMVMKAVEDYAQRKGIKILASGDLLSSGLISIYRSGELVTLNLPAFLTLDKAEIIELIGGKYDLRFGCPLLREAFRRAPSVKRFAIQRVLRELRARAITPEIAEKLILDILSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5B2</accession>
    <name>A0A218P5B2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dolichol-phosphate glucosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_00820</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853238.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5B2"/>
    <dbReference type="GeneID" id="33314768"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00820"/>
    <dbReference type="OrthoDB" id="11098at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04179">
        <property type="entry name" value="DPM_DPG-synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859:SF91">
        <property type="entry name" value="DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859">
        <property type="entry name" value="GLYCOSYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="2">
        <location>
            <begin position="221"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ05976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="440" mass="49000" checksum="AE5B90715FF012DE" modified="2017-09-27" version="1">MDIRLVIFDLDGTLVGAPKPFTQLKEELRSRLLAMGIPEEALGDLTPMYETLQRVSRELGLSFEELYSHLVELETERMEESFLFEGVREVLELLRGRGVKLAVMTRSSRKAALRALEMHGIARYFDLISTRDDVAPEELKPNPGQLRAIIESLKVEPTKTLVVGDHGYDILPAKELGALSVMITSHESGRMSFSVDVEPDFEVPTMVEFKGLIENLLDTYVVVPAYNEELTIGAVLDDLLRYFRMDEIVVVNDGSRDRTGEIARSKGVHVLTHLVNRGLGGALGTGIAYALRRNARLIITFDADGQHLVSDALRVMRPVAEGRADFAVGSRLKGDISEMPFIKRFGNFILDSITALFAGKYVSDSQSGLRCFNRECASRIRITCDRYAVSSEIIIEVAKSGCRIVEVPIKAVYTEYSMKKGTNVLEGVKIALNLLLDKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Q9</accession>
    <name>A0A218P5Q9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853389.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5Q9"/>
    <dbReference type="GeneID" id="33314939"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01670"/>
    <dbReference type="OrthoDB" id="86247at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="108" mass="12032" checksum="AD24184F0658CFBC" modified="2017-09-27" version="1">MKEEHAVGIILTAGIAISLVFRTYLGVAVAALGIPFYLAYAAREQNILAKSRLYDRDLFLMILIAVAVILLFDYFWDPRMGLIAMAVVIPLMALYADRLNARKKGQKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5R1</accession>
    <name>A0A218P5R1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fuculose phosphate aldolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_01575</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853372.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5R1"/>
    <dbReference type="GeneID" id="33314920"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01575"/>
    <dbReference type="OrthoDB" id="18709at2157"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016832">
        <property type="term" value="F:aldehyde-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019323">
        <property type="term" value="P:pentose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050197">
        <property type="entry name" value="Aldolase_class_II_sugar_metab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006208">
        <property type="entry name" value="PRK08333.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22789:SF0">
        <property type="entry name" value="3-OXO-TETRONATE 4-PHOSPHATE DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22789">
        <property type="entry name" value="FUCULOSE PHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="3">
        <location>
            <begin position="7"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="192" mass="20936" checksum="4A996C2597452193" modified="2017-09-27" version="1">MSRVIKAQLVRYSRLAHERGLTAAFGGNLSIRQGNLVFIKATGAVMDDMRAEQVAVIDMSGRQLSGVRPSSEYRLHLAVYRKRPDVRSIAHLHPPYATIAATLLEKAGELPIITPEAEIYLGRIPVAPFRPAGTEELARVVAEVIAGSDAALMAKHGIVTAGRSLREAFYKAELVEESAKLWYLSRKGSSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5S0</accession>
    <name>A0A218P5S0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01715</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853397.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5S0"/>
    <dbReference type="GeneID" id="33314948"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01715"/>
    <dbReference type="OrthoDB" id="99730at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051918">
        <property type="entry name" value="STPP_CPPED1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43143">
        <property type="entry name" value="METALLOPHOSPHOESTERASE, CALCINEURIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43143:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE CPPED1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="315"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="544" mass="59793" checksum="304ECE83C2A6324A" modified="2017-09-27" version="1">MRKVAAAFLLVFIILAAGCISGGNSTTGTTTPTSSPASGGIDFGSYGKGQVLAKWSELADTSKVYVSEGYEDLAKHYFPDAQILPASQYDGGIAILSPKDARTVLRGKPILITVNDYFGYIIYKFGLKFVGPDKGVMAAFNDNGKAHFVFTGTSKAGAGAALEYAMRLREGAKVNVDDIVRSSDFEGAVLKVIGDNDWDGIPDESESWYLTSFKAMEPFIYYWRVVEGENVTVSGGFIRLVNGSTVYIHALGFNVSIRVKNPKNKELTYVVENINPNYVEVSGKVKSVDMGGTEVKVVSSSDEVNIVPKNVSSYRIIALGDHRPGSGTKPPEVFLKIRDEINNDEGVFIIDGGDLVYTGRVDEWAALLKEWKWNKPIFVAPGNHEYRGEGINVFHMLFGPDNYAFSFGNYRYIIINDVEDNYGLSDETFKWLEDQMNLAAERGERPVLVLHAPPIDPRPNGDHAMKPEDAKRLLETMKAYNAFGIFSHIHIYWYGKEDGVQMLITGGGGAPLYAPPDQGGFYHYVRLDMAANGTVSVEPVKVEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5V9</accession>
    <name>A0A218P5V9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">CDP-archaeol synthase</fullName>
            <ecNumber evidence="11">2.7.7.67</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">carS</name>
        <name evidence="12" type="ORF">A3L08_01940</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the formation of CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol (CDP-archaeol) from 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate (DGGGP) and CTP. This reaction is the third ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + CTP + H(+) = CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:25690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58837"/>
            <dbReference type="ChEBI" id="CHEBI:58838"/>
            <dbReference type="EC" id="2.7.7.67"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the CDP-archaeol synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.67" evidence="11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853439.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5V9"/>
    <dbReference type="GeneID" id="33314993"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01940"/>
    <dbReference type="OrthoDB" id="45383at2157"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043338">
        <property type="term" value="F:CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01117">
        <property type="entry name" value="CDP_archaeol_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032690">
        <property type="entry name" value="CarS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002726">
        <property type="entry name" value="CarS_archaea"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003114">
        <property type="entry name" value="PRK04032.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39650">
        <property type="entry name" value="CDP-ARCHAEOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39650:SF1">
        <property type="entry name" value="CDP-ARCHAEOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01864">
        <property type="entry name" value="CarS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="171" mass="18687" checksum="9696ACBCD5D7B4E5" modified="2017-09-27" version="1">MGLLSSLLWAFWYILPAYFANASPVLVGGGRPIDGGRTWRDGRRLFGDGKTWRGFIGGVSIGTLVGIVQYHITPSFYGDLRTAVLLAFLLSFGALLGDLIGSFFKRRANLPRGAPAIGLDQLGFLITALALAYPVKTLSSGQIIFLLVVSPFIHWGANYFAYKMGWKSVPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5W8</accession>
    <name>A0A218P5W8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9 10">DNA repair and recombination protein RadA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">radA</name>
        <name evidence="14" type="ORF">A3L08_02035</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9 11">Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9 11">Belongs to the eukaryotic RecA-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853456.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5W8"/>
    <dbReference type="GeneID" id="33315012"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02035"/>
    <dbReference type="OrthoDB" id="31129at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19515">
        <property type="entry name" value="archRadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002052">
        <property type="entry name" value="DNA repair protein RAD51 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00348">
        <property type="entry name" value="RadA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011938">
        <property type="entry name" value="DNA_recomb/repair_RadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016467">
        <property type="entry name" value="DNA_recomb/repair_RecA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010995">
        <property type="entry name" value="DNA_repair_Rad51/TF_NusA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020587">
        <property type="entry name" value="RecA_monomer-monomer_interface"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003301">
        <property type="entry name" value="PRK04301.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02236">
        <property type="entry name" value="recomb_radA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942:SF30">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN DMC1_LIM15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942">
        <property type="entry name" value="RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14520">
        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005856">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47794">
        <property type="entry name" value="Rad51 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50163">
        <property type="entry name" value="RECA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9 11" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6 9 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="12">
        <location>
            <begin position="114"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="RecA family profile 2" evidence="13">
        <location>
            <begin position="295"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="143"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="NCBIfam" id="TIGR02236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR005856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ06193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="354" mass="38903" checksum="C1F89B079C5422D2" modified="2017-09-27" version="1">MARKTKAEDEIKELEEFEELEVVEESTSSSTTKKKEKEIKTLEDLPGVGPATAEKLREAGYDSIEAIAVASPMELKEIAGISEGAALKIIQAAREAANIGTFMRADEYMEKRSTVGRISTGSKSLDKLIGGGVETQAVTEVFGEFGSGKTQLAHTLAVMVQKPPEEGGLSGSVIWIDTENTFRPERIRQIAEARGLDPDEALKNIYVARAFNSNHQMLLVEKAEEIIKEKAETDRPVKLLVVDSLMAHFRSEYVGRGTLAERQQKLAKHLADLHRLADLYDIAVFVTNQVQAKPDAFFGDPTRPVGGHILAHSATLRIYLRKGKAGKRVARLIDSPHLPEGEAIFRITEKGAED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5X0</accession>
    <name>A0A218P5X0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flavin reductase like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_01980</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the flavoredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853447.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5X0"/>
    <dbReference type="GeneID" id="33315001"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01980"/>
    <dbReference type="OrthoDB" id="8522at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002563">
        <property type="entry name" value="Flavin_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052174">
        <property type="entry name" value="Flavoredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43567:SF1">
        <property type="entry name" value="FLAVOREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43567">
        <property type="entry name" value="FLAVOREDOXIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01613">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00903">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavin reductase like" evidence="4">
        <location>
            <begin position="5"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="177" mass="19942" checksum="1737023B012611A7" modified="2017-09-27" version="1">MKSYRLMYPMRTYLIVSGRGEEANVMAADWVTVVSARPFMVGVAIAPQRHTWKLVKKYGEFVVSVPGLEMLDDVWVAGTKHGPEKLKETSITLVPSKSIETPSIKEALANLECRVVDERDYGDHTWFVGEVVDGSYSETAFPNGRPNLEANFLAHASWVDFVTFEKRIYRPGKANSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P637</accession>
    <name>A0A218P637_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853493.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P637"/>
    <dbReference type="GeneID" id="33315053"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02240"/>
    <dbReference type="OrthoDB" id="84806at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06555">
        <property type="entry name" value="ASCH_PF0470_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.30">
        <property type="entry name" value="Hypothetical protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007374">
        <property type="entry name" value="ASCH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016645">
        <property type="entry name" value="UCP016134"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34204">
        <property type="entry name" value="RNA-BINDING ASCH DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34204:SF2">
        <property type="entry name" value="RNA-BINDING ASCH DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04266">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016134">
        <property type="entry name" value="UCP016134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01022">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ASCH" evidence="1">
        <location>
            <begin position="6"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="116" mass="13542" checksum="91277785B71D396C" modified="2017-09-27" version="1">MATWRMGLQEEYLRAIAEGRKRIEGRLYDEKRQGIRPGDTIIFENKLMVVVKDIRVYSSFREMLEKEGLENVLPGVESIEEGVRVYRRFYSEEKEKKYGVAAIEVEPVGWVGEPLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P645</accession>
    <name>A0A218P645_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Type II secretion protein F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_02190</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853485.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P645"/>
    <dbReference type="GeneID" id="33315043"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02190"/>
    <dbReference type="OrthoDB" id="85883at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.81.30">
        <property type="entry name" value="Type II secretion system (T2SS), domain F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056569">
        <property type="entry name" value="ArlJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042094">
        <property type="entry name" value="T2SS_GspF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402:SF1">
        <property type="entry name" value="TYPE II SECRETION SYSTEM PROTEIN GSPF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Type II secretion system protein GspF" evidence="7">
        <location>
            <begin position="101"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="298" mass="32946" checksum="593FE255F622E974" modified="2017-09-27" version="1">MPRLSSVFIFLIKKIVPEKWMKRYEVFIYSANIGFLAAEYLVVSLLTGVIGALLVYMFSNWIYALAAFIAVSSGMAFGYPYWRITKRIEEMEKMLPDAFFYLASSLRAGISFSEALEELTTANFGPLTDEFRKTVAEIKKGRPTTDALKAFAIRNKRSSVLYRSMMIIIEALERGAPMSDVLVFVGNDVRELLRIKQERRASTGMQVMFFIITSGVIGPLILGVVTQIMASMNVGDINLPVETVKNILLGFVVLQALVSGLGIGVIREGKYSAGLRYSLLLAIMGVIVYQGASGLSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P650</accession>
    <name>A0A218P650_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Metalloprotease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_02295</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853503.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P650"/>
    <dbReference type="GeneID" id="33315064"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02295"/>
    <dbReference type="OrthoDB" id="15212at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031293">
        <property type="term" value="P:membrane protein intracellular domain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06159">
        <property type="entry name" value="S2P-M50_PDZ_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001193">
        <property type="entry name" value="MBTPS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13325:SF3">
        <property type="entry name" value="MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE-2 PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13325">
        <property type="entry name" value="PROTEASE M50 MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE 2 PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01000">
        <property type="entry name" value="SREBPS2PTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="300"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="190"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="377" mass="41099" checksum="529632FE1A2085F4" modified="2017-09-27" version="1">MNTVLITVIAGIAAFWMILYALFGRKEEKEEGLTVDMFIAMWRTKRLLGFIDGISRKNRKLWRVYADVGIALGFMGMGYVFYALLKTAMQTVRQGGQGGGVQLVIPGLTIPLWYGLIGLAVVMIVHELSHGIVARAEGLPLKSVGLVLLAVIPGAFVEPDEEELEKAPLRARLRVYGAGSLANVVTAFIAVLIINLAISPLLQPSGILVSGILDDGPAYGILQNGDVITAMDGVQIQKIDEFIDFMNKTKPGQTVTLTVLRDGKELNVNLKLGAHPENPEKGYIGIYPAQSVTSKVGLDWLVLPLFFAFYWIYVLNIGIGLMNLFPLVPLDGGRMLDDVFKRYLPEGIANPVRYFTIGVGLFLLALNLIPAIMNLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P656</accession>
    <name>A0A218P656_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853534.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P656"/>
    <dbReference type="GeneID" id="33315099"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02470"/>
    <dbReference type="OrthoDB" id="102578at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="320"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="380"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="410" mass="45622" checksum="23F363B947682169" modified="2017-09-27" version="1">MRALLKIESRRLSLSVLIALVLTSITLLADVEISPIVPYIVLGFLLSSVFPAGLSYGLELILSKPLKRIELFAGFLLSHLLLALIVALPALVKPTAFLYAIFALPFLPLDYLTGLLLKNPRKTFLVGTLVFLILTFLPTGYVDMKVQNDAQKALGIETLADYEAKKAEYSSLVYTLERKYGNYAFFSPGVQLELFARDLDVGDKEDALLRAGIALGFTVILLALSIIPFLRFEPGEFIRPSIPALYLRPLPWWIRKEIAGLWASRAFFVFLIVLLLPVDRITKAFFALFLLPLLVIEVLSENPILILSKPVRRAYLIRRFLVVLGLFALFTPVLGFSLAVVSFTATVIFLTGLFSRKLALALLPFFALIISPALQEGSQLVALLFMTLTLPFIALAYLRAIRMELGRWGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P671</accession>
    <name>A0A218P671_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">V-type ATP synthase subunit K</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_02580</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853555.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P671"/>
    <dbReference type="GeneID" id="33315122"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02580"/>
    <dbReference type="OrthoDB" id="66135at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18180">
        <property type="entry name" value="ATP-synt_Vo_Ao_c_NTPK_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000245">
        <property type="entry name" value="ATPase_proteolipid_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006219">
        <property type="entry name" value="PRK08344.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10263">
        <property type="entry name" value="V-TYPE PROTON ATPASE PROTEOLIPID SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00122">
        <property type="entry name" value="VACATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="57"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="135"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="9">
        <location>
            <begin position="9"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="9">
        <location>
            <begin position="97"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="162" mass="16080" checksum="C2BB1BEEF26783C0" modified="2017-09-27" version="1">MDPIVYVSLGAALAAGIAGIASAFGVSAAGAAAAGAVAEDEKNFRNALILEGLPMTQSIYGLITLFLILMSAGIIGGGFKFADPNNMDNIVKSVILFSAGLVVGLTSLSAIPQGIIAGASIGAVAKNPKTFTQGIIFAAMAETMAIFGLVGALIMIATGVGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P695</accession>
    <name>A0A218P695_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="14">6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Alanyl-tRNA synthetase</fullName>
            <shortName evidence="14">AlaRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">alaS</name>
        <name evidence="16" type="ORF">A3L08_02465</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>tRNA(Ala) + L-alanine + ATP = L-alanyl-tRNA(Ala) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="14"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853533.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P695"/>
    <dbReference type="GeneID" id="33315098"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02465"/>
    <dbReference type="OrthoDB" id="7506at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00673">
        <property type="entry name" value="AlaRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.130:FF:000010">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.310.40:FF:000001">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.54.20:FF:000005">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000056">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000002">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.54.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.550">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_A">
        <property type="entry name" value="Ala_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022429">
        <property type="entry name" value="Ala-tRNA_lgiase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050058">
        <property type="entry name" value="Ala-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03683">
        <property type="entry name" value="A-tRNA_syn_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777:SF9">
        <property type="entry name" value="ALANINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777">
        <property type="entry name" value="ALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 14" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 14 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 14" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 14" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="8 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alanyl-transfer RNA synthetases family profile" evidence="15">
        <location>
            <begin position="57"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="614"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="618"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="718"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="722"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ASJ06271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="916" mass="104695" checksum="24F0911502E689BE" modified="2017-09-27" version="1">MSMDMTTRMFKEEGWIRKQCPKCGKFFWTLDPDRETCGDPPCDEYSFIGKPGIPKKYTLDEMREKFLSFFERKGHGRVKRYPVLPRWRDDVLLVGASIMDFQPWVISGEADPPANPLTISQPSIRFTDIDNVGITGRHFTIFEMMAHHAFNYPGKPIYWMDETVELAFEFFTKELGMKPEDITFKENPWAGGGNAGPAFEVLYRGLEVATLVFMQYKKAPENADPSQVVEIKGDYYVPMETRVVDTGYGLERLVWMSHGTPTAYDAVLGYVIEPLKKMAGVEKIDERILMENSRLAGMFDIEDMGDLRYLREQVAKRVGISVEELEKAVRPYELIYAVADHTKALTFMLADGVIPSNVKAGYLARLLIRKSIRHLRELGLEIPLAEIVAMHIKELSPTFPEFKEMEEVILDIINVEEKRYQETLKRGSDLVKREIAKLKKKGINEIPLDKLILFYESHGLTPEIVAEVAQKEGVKVEIPDNFYTLVAKQAEKTEKTEKKTAGEYVVDFELVKDLPDTRTLYYEDPFMKEFDAEVLKVIDGWVVLNQTAFYPEGGGQPYDTGVLVVDGEEVKVTNVQKVGKVILHKVERPELFKEGAKVHGRLDWDRRIQHMRHHTGTHVLMGALVRVLGKHVWQAGSQLHTDWARLDISHYKRITEEELREIERLANRVVMENRKVTWEWLPRTEAEQKYGFRLYQGGVVPGRVIRVLKIEDWDVQACGGTHLPNTGLIGPIKILRTERIQDGVERIIFAAGEAAINWMQETERILKKTAEIFRVPPEKVPETAERFFNEWKEARKEVEKLRKELAKLLVYELENEVERVGEIEFIGKVVEGTIDDLREAANKLRKDKRVIVLITREGHFVVAVGDGLDLKAGELAKVITSVAGGGGGGRKELAQGRIKNPLKAEEAIEEVKKRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6D6</accession>
    <name>A0A218P6D6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_02895</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853614.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6D6"/>
    <dbReference type="GeneID" id="33315185"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02895"/>
    <dbReference type="OrthoDB" id="2837at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10550">
        <property type="entry name" value="DMSOR_beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050294">
        <property type="entry name" value="RnfB_subfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42859:SF10">
        <property type="entry name" value="DIMETHYLSULFOXIDE REDUCTASE CHAIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42859">
        <property type="entry name" value="OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13247">
        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12800">
        <property type="entry name" value="Fer4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="7"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="46"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="80"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="173" mass="18790" checksum="3AF77171272A0021" modified="2017-09-27" version="1">MGDEERLWILVTPDKCSGCRLCEIACSLEHEGIIWPEASRIRVYELLPGVNVPHTCVQCPDYPCVNACNFDALSVDEVTGAVLVNEEKCIECGACVLACPGNVPRIPAGKGSVVICDLCGGEPKCVEVCHEAGHDALTLVKGNYRSIYRTFAKDPIEKSAELARKMYGEELMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6E5</accession>
    <name>A0A218P6E5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Diaminopimelate aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_02770</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853592.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6E5"/>
    <dbReference type="GeneID" id="33315160"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02770"/>
    <dbReference type="OrthoDB" id="24854at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010182">
        <property type="entry name" value="ArgE/DapE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050072">
        <property type="entry name" value="Peptidase_M20A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01910">
        <property type="entry name" value="DapE-ArgE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010589">
        <property type="entry name" value="PRK13983.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808">
        <property type="entry name" value="ACETYLORNITHINE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808:SF32">
        <property type="entry name" value="ARGE_DAPE-RELATED DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7" id="KW-0170">Cobalt</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="9">
        <location>
            <begin position="206"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="422" mass="47637" checksum="0BF02A57B2F29657" modified="2017-09-27" version="1">MSETLDKVSQEIENLRDEMVETLVELIKIPAISPDYGYEGEYDKAQKLLEMIKDWPFDKVEVYNAPDERAKNGARPNILAYYYGEKGEESERLWILTHIDVVPPGDLSKWTVTEPFKPLVKDGKVYGRGSEDNGQSLVASLYAVKAMMHLGIRPKRTIILAFVSDEETGSKYGIGWLMKEHPELFRENDLVLVPDGGNEDGTFIEVAEKSILWFRVKVRGKQVHASMPDKGLNAHRVALDYAYHLDKLLHEKYSEEDELFDPPKSTFEPTMVRGPADSPNIAPGEHEVVFDCRVLPRYSLDDILNDAKVLAEEVKERYRKEIEGKTLPEIEIEVLQRLDAPAPTDPNSEIVVLLKDALRKLRGKEATVGGIGGGTFAAFFRKLRIPAVVWATLDEMAHQPNEYAKIENMVEDAKVMAALALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6E9</accession>
    <name>A0A218P6E9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Thiamine biosynthesis protein ThiS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_02905</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853616.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6E9"/>
    <dbReference type="GeneID" id="33315187"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02905"/>
    <dbReference type="OrthoDB" id="90908at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd17040">
        <property type="entry name" value="Ubl_MoaD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="72" mass="8140" checksum="9E41C1AE64BB8803" modified="2017-09-27" version="1">MRVTLVLYGEHALKHGPRRELEVEEGKSVRELLRELGISPAEHHILVNERKVTDCRLKDGDVVKVLPVVYGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6G4</accession>
    <name>A0A218P6G4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02975</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853629.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6G4"/>
    <dbReference type="GeneID" id="33315201"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02975"/>
    <dbReference type="OrthoDB" id="66108at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="239" mass="25465" checksum="79FEA6B259BF6523" modified="2017-09-27" version="1">MGRLWGSLLAIAGIALWYYGGTQGNVALVNLGIGTIILGIVLAAFPSRGYVDRDALRLSCRDFCGFVENMREGLELRGSPVVIPPYENLPRGGLFLPKNENFSLHLGKFADGAVFITGTEEESGVLMSPPPGWGILEYTLENVGELSGTGVGYASSAVSSVLSALGIGSAEAFEREDGKIELFAKPMCGDPFYADPVLSAMLLGIAMGKGEVLRVESSERANDHVKLILEPLGGIERWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6H2</accession>
    <name>A0A218P6H2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03070</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853646.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6H2"/>
    <dbReference type="GeneID" id="33315220"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03070"/>
    <dbReference type="OrthoDB" id="198670at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF120">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="478" mass="55280" checksum="8049CFB69D511C37" modified="2017-09-27" version="1">MPKNRTKVVVIGLDGANINTANLVGLNTKYIHNFISTIPPYTPPAWTSILTGVTPAKHGIIDFQRIDPKELTVNIATSRDVKYYRISELLSMHGLKSVLINLPMTYPFEGIKFKENTVIVSDWAAPYQEIYPKRLNEKYKEYLIDPPHSWEKHIDNPSGYAKRVEEYTTTRLNIYYDLLEDWDWDLYFIVFSETDWFSHIFPQILEGKDTHLVYGVFKKIKKFIDKAMNIADITFIVSDHGFEIKRKIFYVNEALARLGFLRYNKTKASFVRFGKKIIPSKIAKILATRGTNQMSYATDPQKRKAFMLSHATWGVYVTDKTLKEQVKKALESFPEISKVVLKEEIYTGPHIQLLPDLFIVPKDGIKFSRDLSGKLTETTYQGDHEIHGILGALGEGINSEITFRHLPTVYDIAPTILHIFNFPIPSDMDGRVLMEIFKEDSEFVKRKPKYVDPSYYRIKQKDEKLKKAIKHLKLKGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6H5</accession>
    <name>A0A218P6H5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyl transferase family 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_03080</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853648.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6H5"/>
    <dbReference type="GeneID" id="33315222"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03080"/>
    <dbReference type="OrthoDB" id="132546at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050194">
        <property type="entry name" value="Glycosyltransferase_grp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947:SF3">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="2">
        <location>
            <begin position="180"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="383" mass="44313" checksum="370E4B4EC45C8FF1" modified="2017-09-27" version="1">MKPRVMIVMFPWRSNNPYKFVSEVAHILSFLSEKVIIVSDKKRLDAIIENSSSIVIYDTKFSMHYITEISPAIYSIFLWILKMIYIQLKEALIIAKNRRNIDIVIFYMAYPYYLLPLIIAKFLRKKTIEIVTRSKPSLIDRLYFWILDGISPESRGIIERLNLHKYSQKLLPEGARFIDTEKFKVKRKIKFRENSIGYLGRLTKEKGILQLIDACELIAMKDGTFKCYIAGEGPLLDAVIKRAEKLKKQYNREIIRVLGYLPDEMVPEYLNRIKLFVLLSQHSEGLPTAILESMACGTPVLTTPVGAITDIVTNGYNGFLITDVDPLSIAESIERIHSMNLTEISKNARKVVLSRYTLKKATKRFTHIVSRLQRGDRYDKKYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6H8</accession>
    <name>A0A218P6H8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Elongation factor 1-beta</fullName>
            <shortName evidence="7">EF-1-beta</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">aEF-1beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ef1b</name>
        <name evidence="9" type="ORF">A3L08_03155</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Promotes the exchange of GDP for GTP in EF-1-alpha/GDP, thus allowing the regeneration of EF-1-alpha/GTP that could then be used to form the ternary complex EF-1-alpha/GTP/AAtRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EF-1-beta/EF-1-delta family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853657.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6H8"/>
    <dbReference type="GeneID" id="33315237"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03155"/>
    <dbReference type="OrthoDB" id="84643at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00292">
        <property type="entry name" value="EF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00043">
        <property type="entry name" value="EF1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036219">
        <property type="entry name" value="eEF-1beta-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014038">
        <property type="entry name" value="EF1B_bsu/dsu_GNE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribsomal_bS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004542">
        <property type="entry name" value="Transl_elong_EF1B_B_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00489">
        <property type="entry name" value="aEF-1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001670">
        <property type="entry name" value="PRK00435.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39647">
        <property type="entry name" value="ELONGATION FACTOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39647:SF1">
        <property type="entry name" value="ELONGATION FACTOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00736">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006521">
        <property type="entry name" value="Transl_elong_EF1B_B_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00888">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54984">
        <property type="entry name" value="eEF-1beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation elongation factor EF1B beta/delta subunit guanine nucleotide exchange" evidence="8">
        <location>
            <begin position="5"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="91" mass="10222" checksum="4FFDDC7542617DED" modified="2017-09-27" version="1">MSDFNLVGVIKVMPTDPNVNLDELEEKLKAVIPEKFGLAKVEREPIAFGLVALKFYVLGKDEEGYSYDEVAELFEKVENVESAQVETVSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6L0</accession>
    <name>A0A218P6L0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">GMP synthase [glutamine-hydrolyzing] subunit B</fullName>
            <ecNumber evidence="4 13">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 13">GMP synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">guaAB</name>
        <name evidence="16" type="ORF">A3L08_03285</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 13">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>XMP + L-glutamine + ATP + H2O = GMP + L-glutamate + AMP + diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 13">Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B).</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="4 13"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853680.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6L0"/>
    <dbReference type="GeneID" id="33315263"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03285"/>
    <dbReference type="OrthoDB" id="33844at2157"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003921">
        <property type="term" value="F:GMP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01997">
        <property type="entry name" value="GMP_synthase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000002">
        <property type="entry name" value="GMP synthase [glutamine-hydrolyzing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000208">
        <property type="entry name" value="GMP synthase [glutamine-hydrolyzing] subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00345">
        <property type="entry name" value="GMP_synthase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001674">
        <property type="entry name" value="GMP_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026598">
        <property type="entry name" value="GMP_synthase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025777">
        <property type="entry name" value="GMPS_ATP_PPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00884">
        <property type="entry name" value="guaA_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000848">
        <property type="entry name" value="PRK00074.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922:SF2">
        <property type="entry name" value="GMP SYNTHASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922">
        <property type="entry name" value="GMP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00958">
        <property type="entry name" value="GMP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54810">
        <property type="entry name" value="GMP synthetase C-terminal dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51553">
        <property type="entry name" value="GMPS_ATP_PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13 14" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="6 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 13 14" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GMPS ATP-PPase" evidence="15">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="27"/>
            <end position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ASJ06422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="307" mass="34378" checksum="C179981A09F05A0B" modified="2017-09-27" version="1">MWEKFIEEKVEEIRKTVGDGKAIIALSGGVDSSTAAVLAHRAIGDRLHAVFVNTGFMRKGEPEFVVKTFRDEFGLNLHYVDASERFFSELKGVTDPEEKRKIIGRVFIEVFEEVAKEIDAQFLIQGTIAPDWIESKGKIKSHHNVGGLPERLNLKLIEPLRDLYKDEVRELAKELGLPEKIYNRMPFPGPGLAVRVLGEVTPEKVAIVREANAIVEEEIERAGLKPWQAFAVLLGVKTVGVQGDIRAYKETIAVRVVESLDGMTANAMNVPFEVLQRIAFRITSEIPEVGRVLYDVTNKPPATIEFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6S0</accession>
    <name>A0A218P6S0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF434 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_03555</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853725.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6S0"/>
    <dbReference type="GeneID" id="33315316"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03555"/>
    <dbReference type="OrthoDB" id="60095at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007368">
        <property type="entry name" value="DUF434"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041652">
        <property type="entry name" value="DUF5616"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42252:SF1">
        <property type="entry name" value="DUF434 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42252">
        <property type="entry name" value="DUF5616 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04256">
        <property type="entry name" value="DUF434"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18481">
        <property type="entry name" value="DUF5616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF434" evidence="1">
        <location>
            <begin position="2"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="DUF5616" evidence="2">
        <location>
            <begin position="61"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="215" mass="24533" checksum="52E4617006CDD2F7" modified="2017-09-27" version="1">MLEEAYRDLKYLLNRGYRKKYALDFVANHYRLTLEERHLLARCVFPDSWVEEVRRKLLKLEELAGRTLAIDGFNVLITLESIMEGKGIICEDGLVRDLKYQGRYKLNENTEELLEKIAGAVSGLEVRKAVFFYGRNVPKSGVVKALTEEALKRFGLQSEVHLVKNPDFELKGFETVATADVGVISKVPHVFDLAQYIAQRLGKRPQSFVELLGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6V2</accession>
    <name>A0A218P6V2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853774.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6V2"/>
    <dbReference type="GeneID" id="33315369"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03820"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="85" mass="9710" checksum="64BCED8CEC440DAB" modified="2017-09-27" version="1">MDFDLFMERYGYKMLFGLFGLVLLVIFGILGLYVYAVVRLFGLFVGGLLLVLALVYAFTTGRKLLDARAEAHAKYFYDSRQGKRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6V6</accession>
    <name>A0A218P6V6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thioesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853767.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6V6"/>
    <dbReference type="GeneID" id="33315362"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03785"/>
    <dbReference type="OrthoDB" id="84897at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016289">
        <property type="term" value="F:acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052723">
        <property type="entry name" value="Acyl-CoA_thioesterase_PaaI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42856">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE PAAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42856:SF1">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE PAAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioesterase" evidence="1">
        <location>
            <begin position="40"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="138" mass="15374" checksum="7B09C7DAF654243D" modified="2017-09-27" version="1">MEQRTHRLTSERLVGKPLKLEPGYAEVELRTTKEMAVDEYGLVHGGFTFGLADYAAMLAVNEPTVVLGKAEVKFLKPVKAGEILTAKARVLESNEGGVGDGTSPRRKKLVKAEVFNERNEKVFEGTFHCYVLERHVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6X5</accession>
    <name>A0A218P6X5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Glutaconyl-CoA decarboxylase subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_03900</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853790.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6X5"/>
    <dbReference type="GeneID" id="33315385"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03900"/>
    <dbReference type="OrthoDB" id="85178at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005661">
        <property type="entry name" value="OadB_MmdB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01109">
        <property type="entry name" value="Na_pump_decarbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35806">
        <property type="entry name" value="OXALOACETATE DECARBOXYLASE BETA CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35806:SF1">
        <property type="entry name" value="OXALOACETATE DECARBOXYLASE BETA CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03977">
        <property type="entry name" value="OAD_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015658">
        <property type="entry name" value="MmdB_OadB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="307"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="373"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="401" mass="42147" checksum="1C5BF576F2B77EF0" modified="2017-09-27" version="1">MAGLVGSIVTFFQGMGLLNLTWGNVVMILVGLALVYLAIRYEMEPLLLLPIGISAVLVNIPLGHLANWPVAPNLPEHIADNIFSTLSYLNQQYGPPGIFDIIYYTLIRTELVPLLIFFGLGAMTDFGPMIADPKTALMGAAAQIGVFIAMLTALALGFNLDQAASIGIIGGADGPTTIYLTTKLAPEILGATAVAAYSYMSLVPLIQPPIIRAMTSPEERKIRMEQLRPVSKREKIIFPVVAMIVIGLLVPSAAPLVGMLMIGNLFRESGVVERLSKAAQEELMNIVTIFLGLGVGSTMRADSFLTAQTLMILGLGIVAFASATAGGVLFGKLMMKLSGGRINPMIGAAGVSAVPMSARVVQRIASEEDPGNFILMHAMGPNVAGVIGTAVAAGVFLAVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6X6</accession>
    <name>A0A218P6X6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2240 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03790</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6X6"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03790"/>
    <dbReference type="OrthoDB" id="86203at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018716">
        <property type="entry name" value="DUF2240"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09999">
        <property type="entry name" value="DUF2240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="132" mass="14885" checksum="2659D6793EDDB610" modified="2017-09-27" version="1">MHPLKRAVDYKGSTEFTKSELVGILSFSLRIMGVKEAKEFIAEAVKSGLLTERDGKLVVNEAALDEERKGGDLFEEMVEHIASSLGWERADVIDGINGMRERYGDLDKKVLAYLFGMDKGVDMARFRERLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Z1</accession>
    <name>A0A218P6Z1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03975</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853802.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Z1"/>
    <dbReference type="GeneID" id="33315399"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03975"/>
    <dbReference type="OrthoDB" id="85940at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11570">
        <property type="entry name" value="Protein of unknown function DUF257"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005489">
        <property type="entry name" value="DUF257"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03192">
        <property type="entry name" value="DUF257"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="222" mass="25186" checksum="DEAFAF4441D4B9B0" modified="2017-09-27" version="1">MSLSELFNLLDGAKFGSTVIVENRAPLGAEALLAILLRYTREREIPVFVDDILDAFPLYVKHLNLMGINPDLSSVRVLKMGGNETVGNVVNRGRFDMDPTVYISHYEKLFSSAAPKGEFIDIVLGIDRLFVFQDNLSGTWAILRAMKPIVTNRKRTAFYILESAIMENLRMKPLPVLEDLATSVFELKQEGRMLTVDIRKDPSMFRTKIDTIKLDLREVFSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P706</accession>
    <name>A0A218P706_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Protein translation factor SUI1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_04085</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the SUI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P706"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04085"/>
    <dbReference type="OrthoDB" id="11182at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11567">
        <property type="entry name" value="YciH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.780.10:FF:000006">
        <property type="entry name" value="Protein translation factor SUI1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00604">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050318">
        <property type="entry name" value="DENR/SUI1_TIF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022851">
        <property type="entry name" value="SUI1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005872">
        <property type="entry name" value="SUI1_arc_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002096">
        <property type="entry name" value="PRK00939.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01158">
        <property type="entry name" value="SUI1_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789:SF0">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037511">
        <property type="entry name" value="Transl_init_SUI1_pro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 5" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="SUI1" evidence="6">
        <location>
            <begin position="23"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR037511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="98" mass="11368" checksum="959413AE49CDDAED" modified="2017-09-27" version="1">MPRIVNPLDEMLFKEVLKEQQRIRVYIEKARYGKLKTIIEGIDEKEFDLEDIAKKLKAKLACGGTVKKGRIELQGDHRDRVKKLLADLGFSEDLIEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P708</accession>
    <name>A0A218P708_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein uL15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl15</name>
        <name evidence="9" type="ORF">A3L08_04160</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853836.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P708"/>
    <dbReference type="GeneID" id="33315436"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04160"/>
    <dbReference type="OrthoDB" id="9418at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.990.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_uL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027386">
        <property type="entry name" value="Rbsml_uL15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_uL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_uL15_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00475">
        <property type="entry name" value="RIBOSOMAL_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="8">
        <location>
            <begin position="76"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="148" mass="16439" checksum="6FD4BD3340EF9EB4" modified="2017-09-27" version="1">MIRRKKKVRKLRGSHTHGWGCKKKHRGGGSKGGKGMAGTGKRKDQKFTWTIKYAPDHLGKRGFHRPKAVQYTPQTINLSDIDENLQLFLDAGIAYEEEGKIIVDTTQLGVDKVLGTGRLTRPLVIKAYYVTPKAEEKIKAVGGEVLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P749</accession>
    <name>A0A218P749_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MEMO1 family protein A3L08_04380</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_04380</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the MEMO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853870.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P749"/>
    <dbReference type="GeneID" id="33315480"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04380"/>
    <dbReference type="OrthoDB" id="372162at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07361">
        <property type="entry name" value="MEMO_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.830.10">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00055">
        <property type="entry name" value="MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002737">
        <property type="entry name" value="MEMO1_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04336">
        <property type="entry name" value="AmmeMemoSam_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001987">
        <property type="entry name" value="PRK00782.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11060">
        <property type="entry name" value="PROTEIN MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11060:SF0">
        <property type="entry name" value="PROTEIN MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01875">
        <property type="entry name" value="Memo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53213">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="291" mass="32177" checksum="4AA95DB13B96E02D" modified="2017-09-27" version="1">MIRYPAVAGSFYPADEELVEMLERFFRDLGEEGSERKITAGVAPHAGYVFSGYTASRTYKAIFEDGLPETFVILGPNHTGLGSLIAVYPEGEWLTPLGTIEVDSEMAKAIAKLSGIADLDELAHKYEHSIEVQVPFIQYIVEKAGKDVKLVPIALGIQDEEVSRALGRAIFEASRELGRDVVVIASTDFTHYGSVYGYVPFKARADELPHRIKEWDFQVIRRILDFDVDGMFNEIRKLDHTMCGPGGVGTAIVYSRLAGAVEAELLHYTTSFEVSRSTDAVVGYASIVMRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P752</accession>
    <name>A0A218P752_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_04315</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853859.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P752"/>
    <dbReference type="GeneID" id="33315467"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04315"/>
    <dbReference type="OrthoDB" id="92941at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="108" mass="12265" checksum="7DD5C9EBE4A46FF2" modified="2017-09-27" version="1">MKVFIYNADGLTIPVEVEPGLPFKFRCSEEECGKEVVIEGVVRHADEAEFTEVLESTVTENPDFKKIREITARSLIFEGKVNGKDVVLPVESFDDFAKRFLDEVLVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P777</accession>
    <name>A0A218P777_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_04485</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853887.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P777"/>
    <dbReference type="GeneID" id="33315501"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04485"/>
    <dbReference type="OrthoDB" id="85297at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="148" mass="16743" checksum="A9E95A0BDBF78A56" modified="2017-09-27" version="1">MSMEELYAIAQSELAKDLVFEIDEEPVTVSIKGVLLARADSKTYNFSFFELSDTEFILAVQMKGFIVYLGLEADEEIDEEALPELVRILLQGLTPAIALLITKAEKDYSGRADLLLDDNMSPDLKEFFYSLLLKHRQGKPIYEQTEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7C8</accession>
    <name>A0A218P7C8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Acidic ribosomal protein P0 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">rplP0</name>
        <name evidence="6" type="synonym">rpl10</name>
        <name evidence="9" type="ORF">A3L08_04825</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 50S ribosomal subunit. Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric L10(L12)2(L12)2(L12)2 complex, where L10 forms an elongated spine to which the L12 dimers bind in a sequential fashion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853945.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7C8"/>
    <dbReference type="GeneID" id="33315569"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04825"/>
    <dbReference type="OrthoDB" id="30930at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05795">
        <property type="entry name" value="Ribosomal_P0_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000001">
        <property type="entry name" value="60S acidic ribosomal protein P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00280">
        <property type="entry name" value="Ribosomal_uL10_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050323">
        <property type="entry name" value="Ribosomal_protein_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022909">
        <property type="entry name" value="Ribosomal_uL10_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003096">
        <property type="entry name" value="PRK04019.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003098">
        <property type="entry name" value="PRK04019.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="8">
        <location>
            <begin position="111"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="305"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="313"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="340" mass="36922" checksum="30339D3690DC8CD6" modified="2017-09-27" version="1">MAHVAEWKKKEVEELTKIIKSYPVIALVDVAGVPAYPLSKMREKLRGKALLRVSRNTLIELAIKRAAEELNNPELEKLIDHIQGGAGILATEMNPFKLYKLLEESKTPAPAKPGAVVPHDVVIPAGPTSLAPGPLVGEMQALGIPARIEKGKVSIQKDYTALKAGEVITDQMARILNALGIEPLEVGLNLLAAYEDGIVYTPEVLAIDESEYINMLQQAYMHAFNLSVNTAYPTKQTIEAIIQKAFLGAKNVAVEAGYITKETVEDIFGRALRAVLLIAQNLPEELLDEKTKELLNAQAQMAVAAAPQPTEEKVEEAEEEEEEEEASEEDALAGLGALFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7D6</accession>
    <name>A0A218P7D6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Large ribosomal subunit protein uL1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">rpl1</name>
        <name evidence="13" type="ORF">A3L08_04820</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Binds directly to 23S rRNA. Probably involved in E site tRNA release.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Probably involved in E site tRNA release. Binds directly to 23S rRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Protein L1 is also a translational repressor protein, it controls the translation of its operon by binding to its mRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 11">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11 12">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853944.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7D6"/>
    <dbReference type="GeneID" id="33315568"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04820"/>
    <dbReference type="OrthoDB" id="10382at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.790:FF:000005">
        <property type="entry name" value="50S ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01318_A">
        <property type="entry name" value="Ribosomal_uL1_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_uL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023669">
        <property type="entry name" value="Ribosomal_uL1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023673">
        <property type="entry name" value="Ribosomal_uL1_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003244">
        <property type="entry name" value="PRK04203.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL1M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01199">
        <property type="entry name" value="RIBOSOMAL_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0678">Repressor</keyword>
    <keyword evidence="9 11 12" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8 11 12" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="7 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 11" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="4 11" id="KW-0820">tRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="216" mass="23965" checksum="2191A997B52E4928" modified="2017-09-27" version="1">MAFDRQKIAEAVKEAKARAKPRNFTQTVEMAVNLKDIDLKKPENRFKLEVVLPHGRGKEPKIAVIADGAVAEAAKKLGLDVISGEQLEELAKNPREARKLAKKYDMFIAAAPLMPKIGRYLGKYLGPRNKMPQVVPPTMTNLEPIVARLKRTVRIQLKNNPVVHAPIGTEDMDDEKLAENAEAVLNAILGKLERGENQVKSVYVKTTMGPAVKVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7E7</accession>
    <name>A0A218P7E7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_04920</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853960.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7E7"/>
    <dbReference type="GeneID" id="33315588"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04920"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23528">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23528:SF1">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="321"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="368"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="391"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="213"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="418" mass="46099" checksum="C60AE9BB78534021" modified="2017-09-27" version="1">MERKGFNWGVVLGLALLGFSRSMGWALNKGFSFPLLSDYTTSAFVKGSILALEGFIGLLVPPLLGYYSDTLKSRHGRRRPFIFIGGVLAALATLMIWTAYRMGVPLWGFALTLAFLYFSLHLYTAQFRALMPDTVESGQRGKASGVITLLEWAGNLFLFGLGGFLVAKYGKDHIGPFGLTALFLFIAAAFVYYKVREPDAPEIEENESLTEYVKSIFTMRDFLKFYTAQILWWMSFEFIAIFLYGILAFILYGSATQENIDAVTSMGLYLMALFNVAVLLGSLPGGYIYDKVGRRLSIILGGLIFALPQLWGWFIHTEAQIYAALLVAGIGWGALIAASYPVVGDLLTKFEKEAFTGRYYGVYEATKSIPVLLAGTIGGAIVDLAGGNYRILFPIGAVLVLIAMPLIWSMKELEVSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7G4</accession>
    <name>A0A218P7G4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FAD/NAD(P)-binding oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_04950</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853966.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7G4"/>
    <dbReference type="GeneID" id="33315594"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04950"/>
    <dbReference type="OrthoDB" id="2001at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd19946">
        <property type="entry name" value="GlpA-like_Fer2_BFD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1100">
        <property type="entry name" value="BFD-like [2Fe-2S]-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007419">
        <property type="entry name" value="BFD-like_2Fe2S-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041854">
        <property type="entry name" value="BFD-like_2Fe2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052745">
        <property type="entry name" value="G3P_Oxidase/Oxidoreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42720:SF1">
        <property type="entry name" value="GLYCEROL 3-PHOSPHATE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42720">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04324">
        <property type="entry name" value="Fer2_BFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="BFD-like [2Fe-2S]-binding" evidence="2">
        <location>
            <begin position="406"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="496" mass="54918" checksum="5EF461B6E42BEE47" modified="2017-09-27" version="1">MKTKVAIIGAGISGASIARVLSKYENLEVHLIEKAPDVGWGVSKANTALIHGGYDDDPDKYPIRAKLCIKGNRLWHQWVKELQIPHVWNGALIVALQEEDFDELEKLLERGRRNGVPEMRIIGKEELFHLEPGLTREAIGALWVPIVGQIGPIPAVIALVENAVANGVKTHLETEVRGIKVENGEVKGVETNNGFIEADIVINAAGLHADEIARMAGIDYFEIHPRKGEYFLFDDTIPGPRRVLFPTPTPISKGIVVTTEISGHLMIGPNAQDLPPEEKDNLATTREGLEEVWEGAKKLWPQLPPRSKVIRTFAGLRPEPTGGDFIIKAEEEVWGFINVAGIRSPGLTSAPAIAYEVAEIIQRDLGIKLVEKEKWNPYRREITHFFMLPPEKANEMVKKNPAYGKIVCRCNNVSEGDILEAIERMKFIGVRTPSVDSVKFRTKATTGTCQGSFCRPKIVQLLAREYGVEPWNVTLKGKGSEIGIGDVKALLRRDEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7N1</accession>
    <name>A0A218P7N1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Aldehyde ferredoxin oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_05295</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>tungstopterin</name>
            <dbReference type="ChEBI" id="CHEBI:30402"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AOR/FOR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854023.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7N1"/>
    <dbReference type="GeneID" id="33315663"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05295"/>
    <dbReference type="OrthoDB" id="30771at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.569.10">
        <property type="entry name" value="Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.599.10">
        <property type="entry name" value="Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.9.10">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013984">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013985">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013983">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036503">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001203">
        <property type="entry name" value="OxRdtase_Ald_Fedxn_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036021">
        <property type="entry name" value="Tungsten_al_ferr_oxy-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051919">
        <property type="entry name" value="W-dependent_AOR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30038">
        <property type="entry name" value="ALDEHYDE FERREDOXIN OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30038:SF0">
        <property type="entry name" value="TUNGSTEN-CONTAINING ALDEHYDE FERREDOXIN OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01314">
        <property type="entry name" value="AFOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02730">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00790">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48310">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, C-terminal domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56228">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0826">Tungsten</keyword>
    <feature type="domain" description="Aldehyde ferredoxin oxidoreductase N-terminal" evidence="10">
        <location>
            <begin position="4"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="605" mass="66806" checksum="C7D9C1369227A70B" modified="2017-09-27" version="1">MYGNWGRFLRVNLSTGEVKVEEYGEELAKKWLGSRGLAIYFLLKEMDPKVDPLSPENKLIITPGPLSGTSAPTGGRYNVVTKSPLTGFITMANSGGYFGAELKFAGWDGIIVEGKAEKPVYIYINDDNVEIRDASHLWGKVVSETEEAIRKEIGSKKVRIALIGPAGENLVKFAAIMNDGHRAAGRAGVGAVMGSKNLKAIAVEGSKRVPIADKQKFMLTVREKINKIKNDPVAGGGLPKYGTAVLVNIINENGLYPTRNFQKGVFEHAYEQSGEAMTAKYLIRNQPCYACPIGCGRVNKLPTVGVTEGPEYESTWTFGANLGINDLASIIEANHQCDEFGLDTISTGGTLATAMELYEKGHITDEELGDAPPFRWGNTEVLHYYIEKIAYRKGFGDKLAEGGYRLAESYGHPEYSMSVKKLELPAYDPRGAEGHGLGYATNNRGGCHIKNYMISPEILGYPYKMDPHDISDEKVKMLIIFQDLTALIDAAGLCVFTTFGLGADDYRDMLNAALGWDFSTEDYLKIGERIWNAERIFNLKAGLVPERDDNLPKRFLEEPMPEGPNKGHVVRLKEMLPRYYALRGWTEDGRVPKEKAEELGIAEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7P2</accession>
    <name>A0A218P7P2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Potassium transporter Kef</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_05380</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854040.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7P2"/>
    <dbReference type="GeneID" id="33315680"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05380"/>
    <dbReference type="OrthoDB" id="199127at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="455"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="2">
        <location>
            <begin position="399"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="480" mass="55083" checksum="8592204DAB470E36" modified="2017-09-27" version="1">MCEYVYENGQKCRLKPVEGSKYCPLHIPYDEAERLLGESAGKVKGEAFQRRLKSGYTYFEGVYLYDVKISELKTEKPVVFKNSTIRTILFDSVDVPGVTFYNSRVGRIILFGSRVGTFLLQGSHVFGLNILRASFSNSIYIRDSSVRYVMINSTEYVGTGEKGEEEYGEKKALGRIELSNLSDVRRIGINVRYPLLKRILEEHGINPVSSSERSVKVTDLVLRDIEFDQATRFKRQIRLSVRRLHGGLVLENLNIFGHAEVTGSWLRNPEFIHTRVMGNLIFRRTAFHGDFAWNSTVLPNIPVELSVEGFVDVENCRFSSPRAAEVFYRLARISWERNGDFERADNYYYLEMVAKRNSRLGGRRKGVKRIFIKLEAVFEWLFADLTCKYGTDWKRPILIWLVAVNVFFPILYILTASVEGLSSTMGIMDYEYFSIVTATTLGYGDYHPIGVGRAIASVEALFGMFMWAVFLTVFARKYMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7P4</accession>
    <name>A0A218P7P4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DZANK-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_05470</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7P4"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05470"/>
    <dbReference type="OrthoDB" id="11143at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025874">
        <property type="entry name" value="DZR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12773">
        <property type="entry name" value="DZR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="197"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="DZANK-type" evidence="3">
        <location>
            <begin position="257"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="103"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="301" mass="35415" checksum="1FC4F3D3C018BD3D" modified="2017-09-27" version="1">MEYSRIEKILASLFVAFLLVASINFLRELENVPLRPDYGYYQEKYGIPDLYENQSRLAKLEKELFQVYKGTESNLTEAERLYLFRREEYRVALESGNVTGEKLKQLEDEYLTAKESYEMAYRRYLGAKSAYEKVHSQLEEFNSRIRELEIKANGEYNRAYQAYRLKVLILKLTFVLSLFAASLLLLRRYKNIYTSSFVTYSSLLLLYLILSAIWDTVHTIGLSLFGALATFAALYYIRREYFRPERVYKRRIAQGRCYNCGFPVKDDYLYCPNCGAKLKEKCEHCGALKPIHLEFCPYCGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7R0</accession>
    <name>A0A218P7R0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Potassium transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">A3L08_05515</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TrkH potassium transport family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854065.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7R0"/>
    <dbReference type="GeneID" id="33315707"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05515"/>
    <dbReference type="OrthoDB" id="111943at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015379">
        <property type="term" value="F:potassium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004772">
        <property type="entry name" value="TrkH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00933">
        <property type="entry name" value="2a38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32024">
        <property type="entry name" value="TRK SYSTEM POTASSIUM UPTAKE PROTEIN TRKG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32024:SF2">
        <property type="entry name" value="TRK SYSTEM POTASSIUM UPTAKE PROTEIN TRKG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="143"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="282"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="339"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="402"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="461"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="493" mass="54609" checksum="7C4FF867A12878E6" modified="2017-09-27" version="1">MLELGKHINISDDLFVVKNLIGSILQGVGIAYLIPVLLGWFYPEEIKYVVYFAAPGVFSILLGAWLSRHMGKIEDVNLRQAMVSAAFTWLFASAISVIPFMAIAHMSFVDSYFESMSAWTGTGLTMMSHLESYPRILLFWRAWMQWLGGIGIVLVALTVLIRPGVAAARLYRAEARSERILPNLVNTSKVIFQIYFILTVAGIYLYYINGMPLFDAVTHAMTGLGTGGMSTHDLSIGYFNSTAIEAVTIFLMIMGAVNFTVHYKMFVNRRLKPFFEDIQVRYMFFFLVPTVAITAYSLLQIGDTPADSIRQSVFHAVSAITCTGFGIADLSKYPELGKFILGILMVIGGGAGSTAGGIKLIRVTLMYESLKWTLQQAILPRGAVIKRKVGDYIFTEEDVQEVMSFTMTYIAFLLVGTVYTMIRMGTSLVDAFFEVASAQGNVGLSVGITSPSMPVDMKVLLILHMWIGRLEIFSTLVFIISAFLLVPRVVKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7R5</accession>
    <name>A0A218P7R5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dihydropteroate synthase</fullName>
            <ecNumber evidence="4">2.5.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_05625</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(7,8-dihydropterin-6-yl)methyl diphosphate + 4-aminobenzoate = 7,8-dihydropteroate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19949"/>
            <dbReference type="ChEBI" id="CHEBI:17836"/>
            <dbReference type="ChEBI" id="CHEBI:17839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="EC" id="2.5.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.15" evidence="4"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854085.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7R5"/>
    <dbReference type="GeneID" id="33315729"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05625"/>
    <dbReference type="OrthoDB" id="371861at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004156">
        <property type="term" value="F:dihydropteroate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00739">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="entry name" value="Dihydropteroate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045031">
        <property type="entry name" value="DHP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006390">
        <property type="entry name" value="DHP_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01496">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941">
        <property type="entry name" value="FOLATE SYNTHESIS PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941:SF1">
        <property type="entry name" value="FOLIC ACID SYNTHESIS PROTEIN FOL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="Dihydropteroate synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pterin-binding" evidence="9">
        <location>
            <begin position="11"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="272" mass="29618" checksum="9367089CF29E11F8" modified="2017-09-27" version="1">MKFAGIILDEPKIMGVINVSPESFYKGSVRNDEKKLVETAVKMVEEGAAFIDIGAKSTAPYLETQIPVEEEVRRAVWAVKAVRDSLDVPISIDTTNAKVAEEALKAGADIINDVTGLKGEPEMAKVASEYSAPVVVCAHGRVRDFSDPVHTVIDFLQESLVIAKEHGIEEIAVDPAIGFLRPEVPKWYEWDSKILANLNALKILGRPILIGVSRKSFIGAITGRKDPAERLPGSLSATAIAVFNGANIVRTHDVKETVDAVKMAAFMKRFRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7S0</accession>
    <name>A0A218P7S0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2067 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05585</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854077.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7S0"/>
    <dbReference type="GeneID" id="33315721"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05585"/>
    <dbReference type="OrthoDB" id="85910at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019202">
        <property type="entry name" value="DUF2067"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09840">
        <property type="entry name" value="DUF2067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="210" mass="24607" checksum="1226DBB3DC7567D3" modified="2017-09-27" version="1">MARAKKVITIHVRDDREKEEFMRELQRLRLPAFIYVHGKLNDLKINVQGTKDEIREAIRKIREIHHRVRAKLYPDKRGLYRYTVDDLLREAGASVSTPVLVKTLELLGENVELRDDELVTSMPWEEMVEVTERLGRYLSDVSLNTTRQIREVVLPVSVAFDLDPEEVIDILVDLGAAEWKEDKFKYELVKNKEQALEMLLEHLKGEENED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7S9</accession>
    <name>A0A218P7S9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854079.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7S9"/>
    <dbReference type="GeneID" id="33315723"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05595"/>
    <dbReference type="OrthoDB" id="100870at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="402"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="440" mass="49824" checksum="B54583EF7AD31179" modified="2017-09-27" version="1">MRSRNALALLLIAGLAVRLALAPYSAGSDLTQFYGFAGTMLQHGACFYSYADAVGFSAKDWPYPWPYVYGPVMAYILEGIRALVGGWVETSWGGGVYHVYVDPTWAFAVKLVFIAADVLVALLIYCLLKEKGKKAALLAAAFYYLNPMVIHVSSIYGMFDGLALLFFLLAIYLERREYLSPVLAGFSLTVKHTLLFPAVVLLWEWLLMGRKGLRRVVFFALGVFIPFLPMLLLCPSSLSSIPELMRGMEVSYPLPVSYSMNGLSSLATYLHVTYRMETMPLLRYWYVPAGFLMLLVLMRHSFERDVVTSSTLAYTVFTATYWRVNPQYLLPLVAFLVIMAFHEGRASLVTRALSFIAAVYIGLWPILQPTEFWFQVHMKDPDWTVYRFVDRFTLGVSQEGFYLVYSLILTTLLYMIVLSATLPYLKSLKAWLSERIRVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7V1</accession>
    <name>A0A218P7V1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CBS domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_05810</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854121.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7V1"/>
    <dbReference type="GeneID" id="33315766"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05810"/>
    <dbReference type="OrthoDB" id="85365at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02205">
        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="102"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="164" mass="18434" checksum="6E277ED604C5020B" modified="2017-09-27" version="1">MDVEAALEKFHSMKLAEIIPPTTMMPIVTADSDLLSVLKVLRARHHVWVVKDRESMELVGVIRYLDVIDILLPPESHRFKLGMTSKSLKSLLGGAAKAEDVADRHPLTIEEDATVLDALNKMRRYKAQVLAVVEGEKLVGEVSLRILIDEFLRLLRVGGAQWRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7V8</accession>
    <name>A0A218P7V8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_05775</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854114.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7V8"/>
    <dbReference type="GeneID" id="33315759"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05775"/>
    <dbReference type="OrthoDB" id="132546at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050194">
        <property type="entry name" value="Glycosyltransferase_grp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947:SF3">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="2">
        <location>
            <begin position="17"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="1">
        <location>
            <begin position="195"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="407" mass="46573" checksum="9B51CD707C74007E" modified="2017-09-27" version="1">MEGLKIAIASDWFYPKVGGIESHIDELARNLLFTGHEPYVITHDYRYMKPYVDSFPYRVARFPSTIYFKKYHTSVGVDQLRGINELYKEVGFDITHVHSIYSPLSVAVSKLSRGIRGVPVVATNHSFYGKPPLDSLIGAFVRRNLDRIDTFVAVSTPVADDTRNLLRSKLNGRPVVVVPNGIDVNKWRPPEPEERDKARRDLGVKDEIVILYLGRMTERKQAHRIPFMVRDALRRSNLPKKKVKLVMIGNGPMRPVLEENLRKTGIGEITELYDFMERGRLLPLYWAADLVLMPGILEAFPVVGLEAMATGRPVIGRNESGLSDMVLNGKTGLLAVSEEEMAENLATAFQDREMLVEMGMTARERVEKEFSWEVVLKRILRVYRMTMDMRDEVDRLYLAYKLMRRIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P824</accession>
    <name>A0A218P824_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Leucine/methionine racemase</fullName>
            <ecNumber evidence="6">5.1.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_06150</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-leucine = D-leucine</text>
            <dbReference type="Rhea" id="RHEA:59396"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:143079"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-methionine = D-methionine</text>
            <dbReference type="Rhea" id="RHEA:12492"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57932"/>
            <dbReference type="EC" id="5.1.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.2" evidence="6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854182.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P824"/>
    <dbReference type="GeneID" id="33315833"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06150"/>
    <dbReference type="OrthoDB" id="6534at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018111">
        <property type="term" value="F:methionine racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000004">
        <property type="entry name" value="Acetylornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054934">
        <property type="entry name" value="LeuMetRace"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045634">
        <property type="entry name" value="LeuMetRace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006228">
        <property type="entry name" value="PRK08360.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF58">
        <property type="entry name" value="LEUCINE_METHIONINE RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00051379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00052009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="447" mass="49907" checksum="D18E6F5C5C788C47" modified="2017-09-27" version="1">MEYPRSKEEVVERYSRVFPRSARVTYAPIAAVRAENARVWDIEGREYIDFLSDAAVQNVGHNNPRVVQAIREQAERLVHFTFIYGFPVEPLLLAEKLAEIAPIRAPKVSFGLSGSDANDGAIKFARAYTKRRTILSYIRSYYGATYGSMSITGLDFEVRSVVGELSDVHYIPYPNCYRCPFGKEPKSCKMECVSYLKEKFEGEVYADGVAALFAEPIQGDAGMVVPPEDYFRKVKRILDEHGILLVVDEVQSGMGRSGKWFAIEHFGVEPDIITLAKPLGGGLPISAIIGRGEIMDSLPPLGHTFTLSGNPVASRAALAVIEEIEEKGLLRRAEKLGAYTKKRLEEMKEEHELIGDVRGLGLMLGVDLVKDRETKERAYEEARKVVWRAYELGLVLAFLQGNVLRIQPPLTIEEELLDEGLNRLEKAIADVEEGKVPDSVLAKVQGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P866</accession>
    <name>A0A218P866_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphomevalonate dehydratase large subunit</fullName>
            <ecNumber evidence="9">4.2.1.182</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">A3L08_06360</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of a hydro-lyase that catalyzes the dehydration of mevalonate 5-phosphate (MVA5P) to form trans-anhydromevalonate 5-phosphate (tAHMP). Involved in the archaeal mevalonate (MVA) pathway, which provides fundamental precursors for isoprenoid biosynthesis, such as isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(R)-5-phosphomevalonate = (2E)-3-methyl-5-phosphooxypent-2-enoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:78975"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:229665"/>
            <dbReference type="EC" id="4.2.1.182"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:78976"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer composed of a large subunit (PMDh-L) and a small subunit (PMDh-S).</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the AcnX type II large subunit family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.182" evidence="9"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854221.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P866"/>
    <dbReference type="GeneID" id="33315875"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06360"/>
    <dbReference type="OrthoDB" id="25253at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01355">
        <property type="entry name" value="AcnX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007506">
        <property type="entry name" value="PMDh-L-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36577">
        <property type="entry name" value="DUF521 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G00490)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36577:SF3">
        <property type="entry name" value="DUF521 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G00490)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04412">
        <property type="entry name" value="AcnX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphomevalonate dehydratase large subunit-like" evidence="11">
        <location>
            <begin position="1"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00045120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="386" mass="42085" checksum="E0D29E506615FEBB" modified="2017-09-27" version="1">MYLTKEEELVLAGEYGYALQKAMEILVALGDIYGADRLIPIKSAQVAGVSYKNIGEAGLEFLRDFVNAGAKVSVYTTLNPAGIGDEEFMEKQAEVLNLYREMGIEVTSTCTPYYGANLPKFGDHIAWSESSAVIFANSIIGARTNREGGPSSLAAAIVGKTPNYGLHLEENRKATVIVDVQAKVKTFVDYAALGYHLGRTLGNDVPYLRGIKPEKTEFLKEMGAAMAASGSIALYHVEGETPEYTGAIVDKLETVTVEDEDIRAVKEQFSDDWSGIDMILIGCPHASLPEIKEIAELLAMRGRPLKIPLFITASRAVKALADALGYTETIERYNGKIMADSCFVVSPIKGWYRGIATNSGKSAFYFRSFGFNVRLDDAENLIKEAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8E3</accession>
    <name>A0A218P8E3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Xanthine phosphoribosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_06860</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854311.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8E3"/>
    <dbReference type="GeneID" id="33315975"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06860"/>
    <dbReference type="OrthoDB" id="4952at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363">
        <property type="entry name" value="HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363:SF2">
        <property type="entry name" value="PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="3">
        <location>
            <begin position="12"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="215" mass="24709" checksum="48D11F4A65D48305" modified="2017-09-27" version="1">MKKFPAYLASWDDIERWAKEGAWKVLEDGWIPDVVVGLARGGWVAARLYCDYLGVKDLVSLKVEHWGVTATPDGKAKLKYGSNYDLSGKKVLIVDDISDTGESLTLAKNYVESQKPAEIRVATLLTIRGSRFKPDYYGEEIDWAWIVFPWNFVEDMINLVNNLFEEKDALTTDEIVDLFKELHNMDVPKGKLEEALRMAERRKVFKFREGAWRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8G5</accession>
    <name>A0A218P8G5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_06990</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854335.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8G5"/>
    <dbReference type="GeneID" id="33316001"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06990"/>
    <dbReference type="OrthoDB" id="85585at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18730">
        <property type="entry name" value="PIN_PH0500-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051749">
        <property type="entry name" value="PINc/VapC_TA_RNase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42740:SF2">
        <property type="entry name" value="RIBONUCLEASE VAPC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42740">
        <property type="entry name" value="RIBONUCLEASE VAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-1277">Toxin-antitoxin system</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="163" mass="18317" checksum="287CCB379023FB81" modified="2017-09-27" version="1">MKMPKRIILDSSALLRMHTKRNKDLLELALMRFEILVPQISVYEYLFTKACLGKNPDHVMAVLKELYHIVPPDDEIIIKSAIITKNLLKSRTKMATSDILVGVTAIVKNALLITDIPEHYNPLKKYGLDVISTEKFIGELEQLALGLSKDSSEAISKQLSEGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8I0</accession>
    <name>A0A218P8I0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CAAX protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_06875</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854314.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8I0"/>
    <dbReference type="GeneID" id="33315978"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06875"/>
    <dbReference type="OrthoDB" id="86156at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080120">
        <property type="term" value="P:CAAX-box protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040590">
        <property type="entry name" value="Pyro_CPBP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="2">
        <location>
            <begin position="115"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="200" mass="22882" checksum="874A92870D902524" modified="2017-09-27" version="1">MELRRNPYILYFILLPLILVVRYSDGGVFRWALYNLLFYFAVPLLVGRVMGFRRDELGVQIGKGPSYRWALILFIATIPLSLYGTTVPEMKDYYPIFEYSGWGDFFVKELAVGAIMFAHEAFFRGMLLFPLAKKNEWLAILAQDVPYALAHIGKPGIEVPYSFVAGIIFAKMDLKGESFLPSFLLHWLGSVLFDVLCVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8I9</accession>
    <name>A0A218P8I9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cellobiose phosphorylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_07000</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8I9"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07000"/>
    <dbReference type="OrthoDB" id="96333at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="1061" mass="124383" checksum="9CB6A6D8B89F7617" modified="2017-09-27" version="1">MEKYRFDEKGRFVIEDYNRLRPFASFLPGIAGKKGIPMWVFYVNRGQCVSSFGVQDKDHPIMEFNSAIRAYQTVRTLGFRTFIKLPDEGVAYEAFTPSEGRVKQRMYIGRNELEIEEINKDLGLKINVLYYTLPGERIPALIRRVRIRNISDSPKRIELLDGMPMIIPYGVNDYVIKHMNTLVKAWMEVYNLDKKMPFFKLKSSTEDVPKVTELTEGTFYISLVKKGETSELVRPIVDPDIVFGTDSSLISPENFIRKPLGELASEKQVTFNKIPSAFTPLETELMPGEEVKIHSVIGYVPHIALLEEYEKKFASEEYLEKKYLENSEIIEEVVEDIWTQTASKLFDEYSKQCYLDNVLRGGYPLLLEEENPFVYYIYLRKHGDQERDYNYFVLVPEYYSTGNGNFRDVNQNRRENVFFHPEIRDYDIKFFMNLIQADGYNPLVINGVKYRLKTERTDFLKELVDNPEPLEEFLREPFTPGRLMMFVEEKGIELKVSEEEFLRRVLEHCEEEVDAVHGEGYWCDHWTYNLDLIESYLGVYPEKKRELLFEDYSYTYYDNAMVVLPRSKRYVLENGRVRQYNSLVEDKEKKALIESRKEYRHLMRTANGRGEVYRTNLITKLLNLAFIKFATLDPAGMGIEMEAGKPGWYDALNGLPGLFGSSVGEAAELVRLFEFLIDSLEEFPDIELRIPVEVWELFEEEARLVEEYNRREDEDRDHRLWESLSELRERYRDRTRLGFDGKEVEVKARELLDGLKLLRDKLRAGLERAIEENSGIMPMYFYYEPVEYEVNEKGEIRILKFEQKRMPLFLEGAVKQFKIFRNDRELLKEVYKKVKESDLYDKKLKMYKLNASLKDQPIEIGRARAFTPGWLENESIWLHMEYKYMLELIRSGLYEEFYEDFRNVIVAFLDPAVYGRSPLENTSFIVSSAYPDESLHGAGFYARLTGANAEFLSIWKNMFIGEKPFDIENGELVLSFRPALPGWLFDEEGKVSFKLFSRCRVTYHNPAKVDTWKLDLGKARILLHLDDGRKVEVEGNKVRGELARAVRDGKVASIDVYFPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8K9</accession>
    <name>A0A218P8K9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nucleotidyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_07165</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the HepT RNase toxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8K9"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07165"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110001">
        <property type="term" value="C:toxin-antitoxin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.580">
        <property type="entry name" value="bsu32300-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008201">
        <property type="entry name" value="HepT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037038">
        <property type="entry name" value="HepT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051813">
        <property type="entry name" value="HepT_RNase_toxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34139:SF1">
        <property type="entry name" value="RNASE MJ1380-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34139">
        <property type="entry name" value="UPF0331 PROTEIN MJ0127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01934">
        <property type="entry name" value="HepT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-1277">Toxin-antitoxin system</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="71" mass="8441" checksum="18391761E5773924" modified="2017-09-27" version="1">MSMIRRLEDIEGVCKDIRREYPTVPWRRIVGLRNVVVHHYFGVDLSVVWVIVSSQLEELKEEIEKIIETEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8L7</accession>
    <name>A0A218P8L7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">Ferrous iron transport protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">A3L08_07245</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854382.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8L7"/>
    <dbReference type="GeneID" id="33316052"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07245"/>
    <dbReference type="OrthoDB" id="85305at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01879">
        <property type="entry name" value="FeoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1770:FF:000003">
        <property type="entry name" value="Ferrous iron transport protein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000969">
        <property type="entry name" value="Ferrous iron transporter B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003373">
        <property type="entry name" value="Fe2_transport_prot-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011640">
        <property type="entry name" value="Fe2_transport_prot_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041069">
        <property type="entry name" value="FeoB_Cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050860">
        <property type="entry name" value="FeoB_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030389">
        <property type="entry name" value="G_FEOB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00437">
        <property type="entry name" value="feoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43185:SF1">
        <property type="entry name" value="FE(2+) TRANSPORTER FEOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43185">
        <property type="entry name" value="FERROUS IRON TRANSPORT PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07664">
        <property type="entry name" value="FeoB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17910">
        <property type="entry name" value="FeoB_Cyto"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02421">
        <property type="entry name" value="FeoB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51711">
        <property type="entry name" value="G_FEOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0410">Iron transport</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="290"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="346"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="390"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="424"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="455"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="514"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="566"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="601"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="631"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="FeoB-type G" evidence="17">
        <location>
            <begin position="3"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="10"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="35"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="56"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>3</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="NCBIfam" id="TIGR00437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR603373-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR603373-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ASJ07131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="659" mass="73119" checksum="BD11B8053DF780E7" modified="2017-09-27" version="1">MAMKVVALAGNPNVGKTTIFNALTGMRQHVGNWPGVTVEKKEGVFEYNGQKFLVVDLPGTYSLTAHSIDELVARDFLLKGSADVVVNVVDATALMRNLFLTMEILEMGLNNVIIALSKIDLAEKHGVEINIKRMEETLGVPIVPVNAKEGIGLDELKKKIYEMANGMVKERPVIPKYDPEVEREIEHITLALRETPLAEEYNLRWLAIKLLQRDDGVIKLVLRHLGSEKLDEIMGHIAEVEERYKRAMDLIIASQKYEFIDRLMHRFVRYTKTEGKSFSDQLDGILTHPVYGLIALFGVFYLVFKFVFAFGLPLQELLDGAFSYFGEWLAPHITNEALRGLIVDGIIAGVGSVLSFFPLVFLLFFALSILEDVGYMARAAVVMERIMRKFGLPGKSFIPLVLAFGCNVPAVMATRTLDDERDRLVTMLVNPLIPCSARLSVISFLAAAFFAGRQALVAVSIYATAVLIAFVVAWLLSRFVIKGEESPFIIELPEYLVPGWKTVILHSWERSKEFIKKAGTIILLGAIAIWYLSNYPVPLGSGGSYAEKLGYFFEPYMRLMGLDWKAAVSLLFGIIAKENVISTYGVIYGNEAAIASVMTPLQAYVLAMVTTLYVPCIATIGAIRAESNWKWAAFAVAYMIALASIVGIIIWNVGSALGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8M2</accession>
    <name>A0A218P8M2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854392.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8M2"/>
    <dbReference type="GeneID" id="33316063"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07300"/>
    <dbReference type="OrthoDB" id="85618at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="360"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="384"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="409"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="459" mass="52243" checksum="5469F682BC7E2531" modified="2017-09-27" version="1">MDWRKVTFALILLITMAGSFWYVYDFASQPVFHDYVSDEVWYVPASRNILHRLGVTLTYVNESSGSMGVNVIFLNQSVGVKYQYDVEKIALRHGATYEKEYLKFPGVYFEIPPEQFDAFLDDLSSSLPGEVYYVVPGFWYPDKENIQDYLNTEHPFLGKDFIMLGMLIEDRPVNWRVPGIVAFVLTELLVALATYRISKSYLAAIIALVFTVADPTLQAMSTVAMLDIYVAFGVALFVFFLAYEKDRWAAFAAGLAGATKLSGGFGWPVLLVRAFKREKSIVGFLLTVFVLPALGFLLPNVPAMVAVGPEKWLRDFLGSFKWHLSNKGGHPAASPVWEWFVNKKAFALHYDPNVFAQTDPFLLLSMVLFIFALPWLYRRKNGLLAPFSIFWSTVFFFTLQYVLGGTTQFSFYATALVPPAAVVMGVALRELLRWEAFTNSLWLYLEWLLEVKDSVRLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8M4</accession>
    <name>A0A218P8M4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycine--tRNA ligase</fullName>
            <ecNumber evidence="9">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glycyl-tRNA synthetase</fullName>
            <shortName evidence="9">GlyRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">glyS</name>
        <name evidence="11" type="ORF">A3L08_07170</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the attachment of glycine to tRNA(Gly).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Gly) + glycine + ATP = glycyl-tRNA(Gly) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:16013"/>
            <dbReference type="Rhea" id="RHEA-COMP:9664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9683"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.14"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="9"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854368.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8M4"/>
    <dbReference type="GeneID" id="33316037"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07170"/>
    <dbReference type="OrthoDB" id="6113at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006426">
        <property type="term" value="P:glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00774">
        <property type="entry name" value="GlyRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00858">
        <property type="entry name" value="GlyRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000179">
        <property type="entry name" value="Glycine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000002">
        <property type="entry name" value="Glycine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.720.200:FF:000001">
        <property type="entry name" value="Glycine--tRNA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00253_A">
        <property type="entry name" value="Gly_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027031">
        <property type="entry name" value="Gly-tRNA_synthase/POLG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022960">
        <property type="entry name" value="Gly_tRNA_ligase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033731">
        <property type="entry name" value="GlyRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002315">
        <property type="entry name" value="tRNA-synt_gly"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00389">
        <property type="entry name" value="glyS_dimeric"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003211">
        <property type="entry name" value="PRK04173.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745:SF0">
        <property type="entry name" value="GLYCINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745">
        <property type="entry name" value="GLYCYL-TRNA SYNTHETASE/DNA POLYMERASE SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01043">
        <property type="entry name" value="TRNASYNTHGLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="10">
        <location>
            <begin position="6"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="197"/>
            <end position="199"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="207"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="212"/>
            <end position="216"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="324"/>
            <end position="325"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="439"/>
            <end position="443"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="443"/>
            <end position="446"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ07118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="570" mass="66414" checksum="A3E7138CAB9D23DC" modified="2017-09-27" version="1">MGEKPDKYEVLQDLMRRRGFAWGSFEIYGGSRGFYDYGPLGAAIKRKIERKIREAFQREGFFELETPDITPEKVFIASGHVEKFVDPLVECQKCGARFRADHLVEEALGIDTEGMSAEHLTQLIREHDIRCPECGGELGEVWYFNLMFETKIGPYGDQKGYLRPETAQGIFVNFRRLNAFARNRLPFGVFQIGKAYRNEISPRQGMLRLREFTQAEAEIFFNPNETEHPHFDEVKDEVLRLYPIEHQLKNLGEIEITAEEAVKKGYIMNTFFAYYMVMVKRVLLDIGIPEDKIRFRQQLPEERAHYSRDTWDAEIYSERFGWVECVGVANRGDYDLSKHMKMSGADLTVLIHYDEPKIVKRLKVSLNMKRVGPKLKKDAKRINDLIQSWDEEKKRELVELLEKNGKIEIEGYELEKDDFIIKEVEEKVTGEKIVPHVLEPSFGIDRPFYLLLENSLVIEEDRTYLRLKKDMAPIEVAVLPLVAKEPLKSIAYEISRTLQKAGFIAVYDEKDTVGRRYMRYDEIGTPYCVTVDNQTPEDGTVTIRDRDTREQVRVKIEELPEKLRELIFGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8N7</accession>
    <name>A0A218P8N7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ATP pyrophosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_07230</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854379.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8N7"/>
    <dbReference type="GeneID" id="33316049"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07230"/>
    <dbReference type="OrthoDB" id="61764at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012122">
        <property type="entry name" value="ATPase_PP-loop_MJ1016"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052188">
        <property type="entry name" value="Ni-pincer_cofactor_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018317">
        <property type="entry name" value="QueC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43169:SF4">
        <property type="entry name" value="ATPASE, PP-LOOP SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43169">
        <property type="entry name" value="EXSB FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06508">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036670">
        <property type="entry name" value="ATPase_UCP036670"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="266" mass="30735" checksum="0E2ABF946B43DDE2" modified="2017-09-27" version="1">MPSIREVTREIEAFSRTNGLYKKKILVLFSGGKDSSLVLYLLKKAGLDVSALTFFHRWSWRETLNWAMGFTKKLGVEHFLVDVTDGLLREAAGRKGPVCINCKKVMLWNAKWFALNNGFDVLAKGDNANDKIIGALLDQCKTDIRLCGLPKIGVPFFRPLIKYTAEEVEALAEEVGIKPHRMYEHARRRQWREGCPLQYIDREEIVTPELMELAYRVNYEVSKIARKRKVRVSVRVPSFEVMCWGCDSETLREVEFVIGRFKEDKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Q5</accession>
    <name>A0A218P8Q5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fe2+ transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_07240</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854381.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Q5"/>
    <dbReference type="GeneID" id="33316051"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07240"/>
    <dbReference type="OrthoDB" id="105333at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007167">
        <property type="entry name" value="Fe-transptr_FeoA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053184">
        <property type="entry name" value="FeoA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038157">
        <property type="entry name" value="FeoA_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008988">
        <property type="entry name" value="Transcriptional_repressor_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43151:SF2">
        <property type="entry name" value="FE(2+) TRANSPORT PROTEIN A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43151">
        <property type="entry name" value="FEOA FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04023">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00899">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50037">
        <property type="entry name" value="C-terminal domain of transcriptional repressors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferrous iron transporter FeoA-like" evidence="2">
        <location>
            <begin position="3"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="75" mass="7823" checksum="4B97E1B4C7BBCF2E" modified="2017-09-27" version="1">MIVPLNSLKPGERGVVVNILGGPTARQRLVGMGLTPGATIQIIEAHSHGPIIISVGGVRFAIGRGMADKVMVRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8R6</accession>
    <name>A0A218P8R6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854406.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8R6"/>
    <dbReference type="GeneID" id="33316077"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07370"/>
    <dbReference type="OrthoDB" id="8895at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008832">
        <property type="term" value="F:dGTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006203">
        <property type="term" value="P:dGTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050135">
        <property type="entry name" value="dGTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045509">
        <property type="entry name" value="HD_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373:SF4">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE SAMHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19276">
        <property type="entry name" value="HD_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD/PDEase" evidence="1">
        <location>
            <begin position="46"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="358" mass="41508" checksum="1DA1E71205910DE8" modified="2017-09-27" version="1">MKLVHDPIHGHIELDDFAVRLVDTPEFQRLRRITQLGLVYLAYPSARHTRFEHSLGAFHLARRIREHNPDIEEGAVYAALLHDLGHYPFSHTLEALYPRHEEGTRWIIKNGEVGDVIRERYSLREFFELLKHPLVSGDIDADRMDYLVRDAYYTGIAFGLVDLDRLIRNLRWDGEKLVVGEKGTMAAQNLLLARSMMYPAVYQHHTSKIAGAMLVKAVELEGISYEEIRTMDEIDLVSRLRTSKKPEVRELMKAIDSRKLYKRVVYTNEDPGADAVSELKRELEAEFSHLAIVDYSPKPKFEERNAFVETEEGLKRLSDVSPLVRSLVELKDTHWHWGVYAREDVRERVLRAVNKLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8R7</accession>
    <name>A0A218P8R7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Molybdopterin synthase sulfur carrier subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_07395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854409.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8R7"/>
    <dbReference type="GeneID" id="33316082"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07395"/>
    <dbReference type="OrthoDB" id="98357at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd17040">
        <property type="entry name" value="Ubl_MoaD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="76" mass="8443" checksum="9ABB82C5EC29CB02" modified="2017-09-27" version="1">MARIKLMGAFAHLAGARELNVKVDGKKTVDEILRELIPRYDEFHDKIIMINGHPARGDAEVEDTDEIKVMPVLSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8S0</accession>
    <name>A0A218P8S0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Probable deoxyhypusine synthase</fullName>
            <shortName evidence="11">DHS</shortName>
            <ecNumber evidence="6 11">2.5.1.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">dys</name>
        <name evidence="12" type="ORF">A3L08_07610</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 11">Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>[eIF5A protein]-L-lysine + spermidine = [eIF5A protein]-deoxyhypusine + propane-1,3-diamine</text>
            <dbReference type="Rhea" id="RHEA:33299"/>
            <dbReference type="Rhea" id="RHEA-COMP:10143"/>
            <dbReference type="Rhea" id="RHEA-COMP:10144"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57484"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:82657"/>
            <dbReference type="EC" id="2.5.1.46"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 11">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 11">Protein modification; eIF5A hypusination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 11">Belongs to the deoxyhypusine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.46" evidence="6 11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854446.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8S0"/>
    <dbReference type="GeneID" id="33316125"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07610"/>
    <dbReference type="OrthoDB" id="17730at2157"/>
    <dbReference type="UniPathway" id="UPA00354"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034038">
        <property type="term" value="F:deoxyhypusine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.910.10:FF:000004">
        <property type="entry name" value="Probable deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.910.10">
        <property type="entry name" value="Deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00153">
        <property type="entry name" value="DHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022899">
        <property type="entry name" value="Deoxyhypus_synthase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002773">
        <property type="entry name" value="Deoxyhypusine_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036982">
        <property type="entry name" value="Deoxyhypusine_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00321">
        <property type="entry name" value="dhys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003052">
        <property type="entry name" value="PRK03971.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703:SF0">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01916">
        <property type="entry name" value="DS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0386">Hypusine biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0520">NAD</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ07195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="336" mass="38156" checksum="CB8113F269839147" modified="2017-09-27" version="1">MTEPKDIVLKESEEVEGTPIEGPWLDEVSSLEEVLDYYERIGFQATHLGKAIEIWRKVERKRAEGKEIRVFLGYTSNIVSSGLRELIAWLVKEGKVDVIVTTAGGIEEDFIKALKPFVLGDWNVNDALMREKGINRIGNIFVPNDRYIEFEKYMIPFFERVLEMEKERGKPLTASEFIYELGRYMDEKLGKEKERSIIYWAYKRNVPIFCPAITDGSIGDMLYFFKEERGDRELIIDIANDIVKLNNLAVTAKETASIILGGSLPKHAIINANLFRGGTDYAIYVTTAIPWDGSLSGAPPSEGVSWGKIRAKADYVEIWADATLVFPLLVWKVMRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8U3</accession>
    <name>A0A218P8U3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ribosomal protein-serine acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854449.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8U3"/>
    <dbReference type="GeneID" id="33316129"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07630"/>
    <dbReference type="OrthoDB" id="120213at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43328">
        <property type="entry name" value="ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43328:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="11"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="176" mass="21265" checksum="67EA3EF1208DBD44" modified="2017-09-27" version="1">MRPILLKGNLVSLGILLREDLRQVWLWYNDREVRRYLAFPEEIFFYEDELEWYETLRREKRGEKVFSILENSSRSLVGLVGLHRIDYHNGHAELGYFLAREHWGHRYGSEAVKLALEYAFTWLNLRKVYARVYEPNLPSIRILEKNGFQLAGRWKRHQYVPGEGFVDVLLYERFRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8W1</accession>
    <name>A0A218P8W1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ABC transporter permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854467.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8W1"/>
    <dbReference type="GeneID" id="33316152"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07750"/>
    <dbReference type="OrthoDB" id="86030at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056926">
        <property type="entry name" value="FLQE3_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24686">
        <property type="entry name" value="FLQE3_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="233" mass="25857" checksum="09CA958F30641B8D" modified="2017-09-27" version="1">MIGRLVKTNLTLGVRGYVYPIYVLIGLAYGLMLMIFPERYLPTMVPIFLLFEPGLVGFMFVGTEIFAEKKDGAIGALAVTPIEWRDYIIAKTLIMSLLSVIGAVLIMAIGTRSLDGLGYVAVGAFLASVVYTLLGIAVSAKYRDLDDYFVPIMAVLIVSLLPFAHYHGYLTSEIWKVLYAVPSYPVLYFFKAPFVEVSTDTLVKSGIALVVWAGVAYYLAKRRFYKYAVEGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Y2</accession>
    <name>A0A218P8Y2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fluoride-specific ion channel FluC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">fluC</name>
        <name evidence="8" type="synonym">crcB</name>
        <name evidence="9" type="ORF">A3L08_07825</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Fluoride-specific ion channel. Important for reducing fluoride concentration in the cell, thus reducing its toxicity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>fluoride(in) = fluoride(out)</text>
            <dbReference type="Rhea" id="RHEA:76159"/>
            <dbReference type="ChEBI" id="CHEBI:17051"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:76160"/>
        </physiologicalReaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="8">Na(+) is not transported, but it plays an essential structural role and its presence is essential for fluoride channel function.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 8">Belongs to the fluoride channel Fluc/FEX (TC 1.A.43) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854481.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Y2"/>
    <dbReference type="GeneID" id="33316169"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07825"/>
    <dbReference type="OrthoDB" id="253428at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062054">
        <property type="term" value="F:fluoride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140114">
        <property type="term" value="P:cellular detoxification of fluoride"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00454">
        <property type="entry name" value="FluC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003691">
        <property type="entry name" value="FluC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00494">
        <property type="entry name" value="crcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259">
        <property type="entry name" value="FLUORIDE EXPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259:SF1">
        <property type="entry name" value="FLUORIDE EXPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02537">
        <property type="entry name" value="CRCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="35"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <note>structural</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="123" mass="13505" checksum="4A95681D66A63570" modified="2017-09-27" version="1">MNARIIIAIMTGGALGALTRFYLSGILPVYRDFPVGTLLVNSLASLILGYLYGLMFWGLDVPADWRAFFGTGFCGALSTFSTFSYETFSLLREREYLMAGINVAANVIITIALVFVGFILARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Z2</accession>
    <name>A0A218P8Z2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">4-vinyl reductase 4VR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_07970</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854509.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Z2"/>
    <dbReference type="GeneID" id="33316199"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07970"/>
    <dbReference type="OrthoDB" id="371687at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004096">
        <property type="entry name" value="V4R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637:SF1">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02830">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00989">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4-vinyl reductase 4VR" evidence="3">
        <location>
            <begin position="325"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="390" mass="44410" checksum="A4C68224D16C4CB3" modified="2017-09-27" version="1">MERLDEILGGGIREGLNVALIGSFDEDNLLLMHQMAYNLLKSGHRILVAEFRQAPDVLKEWLSYYGIKYDDYIENGRLKILDGFTNLYSSQPVRGEGIIPNPRDLGITAGIIQNELTSGNYDVLIIDDLTVLYALHSDKDAYIRVIIRFINSIKKLGRQVFAGINSEVTEKGDLAKLLLPFEYVIEVANGRIKPLRSYYPLRLPAGHIPYRKTENGLESTWDSYLSLQELKNSLYLDDEGNLWSGSDKVQIINEESEASLIEFVYHYLGPGEGRKFLYLWGRYEFRGVGEAYKNIHESLRAVLEDIAASTETSGGGRLEIVQLEDDLVVLRGRNLFPRIKGFKHPAHVNYAGEIAQLLEEYTGEKWEGDETRCQAMGHNYCEFVFRRKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P917</accession>
    <name>A0A218P917_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Formylmethanofuran dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_07995</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854514.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P917"/>
    <dbReference type="GeneID" id="33316204"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07995"/>
    <dbReference type="OrthoDB" id="31120at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026328">
        <property type="entry name" value="FmdE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003814">
        <property type="entry name" value="FmdEsu_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053194">
        <property type="entry name" value="tRNA_methyltr_O"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000962">
        <property type="entry name" value="Znf_DskA_TraR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39418:SF1">
        <property type="entry name" value="DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39418">
        <property type="entry name" value="DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02663">
        <property type="entry name" value="FmdE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01258">
        <property type="entry name" value="zf-dskA_traR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006578">
        <property type="entry name" value="FwdE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143555">
        <property type="entry name" value="FwdE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Formylmethanofuran dehydrogenase subunit E" evidence="5">
        <location>
            <begin position="23"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger DksA/TraR C4-type" evidence="4">
        <location>
            <begin position="181"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="230" mass="25928" checksum="105B3E45F3D6F0C3" modified="2017-09-27" version="1">MLTLNRLVEERDAEGILEYAREFHGHVCPYLALGIRASLLAMEELGVKRLDYSGSVDESILAIVEVNSCFTDGVQVTTGCTLGNNSLIYLDLGKTALTLVKRSTWEGVRIYADAERLARYYPPGARELFNKVIRERRGTPEERAKLRELWEEAAHRMLHLPREEFKVERVKVPPIEQAPIFDSVRCSKCGELVMETRAVHVDGETFCLRCAGERYLGVVGRGIIELGGME</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P921</accession>
    <name>A0A218P921_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Beta-glucosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_08140</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854537.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P921"/>
    <dbReference type="GeneID" id="33316234"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08140"/>
    <dbReference type="OrthoDB" id="84443at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018120">
        <property type="entry name" value="Glyco_hydro_1_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF209">
        <property type="entry name" value="GALACTOLIPID GALACTOSYLTRANSFERASE SFR2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00572">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="421" mass="49375" checksum="4E4F5EE5DA7FF810" modified="2017-09-27" version="1">MALKFPEGFLFGTATAAHQIEGENRWNDWWYYEQIGKLPYKSGKACNHWEKYEEDIGLMAELGYNAYRFSIEWSRIFPEEGRLNEDALNRYTEILELLRRKGIEPNVTLHHFTSPLWFMKKGGFLREENLKYWEKYVETVADILKGVKLVATFNEPMVYVMMGYLTAYWPPFVKSAFKAFKVAANLLKAHAIAYETLHGSFNVGIVKNVPVMLPASDSERDRKAAQKADNLFNWNFMDAIWSGNFRGAFKSYRVPESDVDFIGINYYTAAEVRHSWNPLKFFFDARDAEIGDRKTQMGWSVYPEGIYRVISKMAGYKKPMYITENGIATLDDEWRKEFIVQHLQYVHKAISEGYDVRGYFYWSLMDNYEWREGFEPRFGLIEVDYDTFERKPRESAYLYGEIAKKGEIGEELIKKYGLKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P931</accession>
    <name>A0A218P931_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854488.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P931"/>
    <dbReference type="GeneID" id="33316177"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07860"/>
    <dbReference type="OrthoDB" id="102662at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="116" mass="12383" checksum="BDB5725890AA1864" modified="2017-09-27" version="1">MGDPSSARNLIHIGDIINLVIGVLVFLVIVVFSFGLGIVVAWIPLAIAIWTHLRAKSALELIDEGRYKEAKDKILIPAILSLIFNSLVGGLIILLGAISLPSENASNPVEGTVEPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P945</accession>
    <name>A0A218P945_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ferritin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_08125</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854534.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P945"/>
    <dbReference type="GeneID" id="33316230"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08125"/>
    <dbReference type="OrthoDB" id="4859at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01055">
        <property type="entry name" value="Nonheme_Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.10:FF:000001">
        <property type="entry name" value="Non-heme ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001519">
        <property type="entry name" value="Ferritin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041719">
        <property type="entry name" value="Ferritin_prok"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431:SF127">
        <property type="entry name" value="BACTERIAL NON-HEME FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431">
        <property type="entry name" value="FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0409">Iron storage</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="5">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="173" mass="20175" checksum="8C182962BD8D7D6A" modified="2017-09-27" version="1">MLSERMLKALNEQMNKELYSAYLYFAMAAYFDDLSLEGFANWMKAQAEEELGHALRFYNYIYDRNGRVELGEVPKPPKEWESPVKAFEAAYEHEQFISRSIHELAALAEEEKDYPTRAFLEWFINEQVEEEANVKKILDQLKLAQDSPQMVFMLDRELGARAPQLPGLLLQGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P952</accession>
    <name>A0A218P952_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_08295</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854567.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P952"/>
    <dbReference type="GeneID" id="33316265"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08295"/>
    <dbReference type="OrthoDB" id="98904at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="104" mass="11507" checksum="2EDE90C5485F5FF5" modified="2017-09-27" version="1">MEVVTNSFGLKSKPILAPKAFEAIYSMWGKIVEGSQLAVPHLVGRARILNGVASLVEMDVVLHTEERPLIIREGNEVSFIVPVEPREGPEGIYLKLQNALEEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P973</accession>
    <name>A0A218P973_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854581.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P973"/>
    <dbReference type="GeneID" id="33316280"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08370"/>
    <dbReference type="OrthoDB" id="97597at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="328"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="349" mass="38318" checksum="B26AE23D99BE1EAD" modified="2017-09-27" version="1">MERVSKLFVLLFFVLLVLSPPVSATPYWFKEGIYAKYVARGWLSIDLNTSTGNVTYYCPRVEFTWRVLNVSGDIGRVSLLLLGFNCTREAYSTLSLEEATTLLRKYQERYNFTGGGCLEVPVAGGNVTVCEESYSEQTAQGSFGLMITDGKGRLFNKSYVPENFSRAGVVEIDLTTGEIHVNGTPVGGNFLWAENPANVTGLEILPGLKIETVKMINSTAMTYYGDFNAPVYMTHTNMLNLKWVVGKDVILYDGSSGLAIALYTPFSPLWKALGVSSAMIQDTEFAREHAKEIKEGNKMPPFGLVLAETNIDFTKPAELPDEGPSKTAIAAAVGVAAILVALVLWRWGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P978</accession>
    <name>A0A218P978_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_08405</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854588.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P978"/>
    <dbReference type="GeneID" id="33316287"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08405"/>
    <dbReference type="OrthoDB" id="3015at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006835">
        <property type="term" value="P:dicarboxylic acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3860.10:FF:000001">
        <property type="entry name" value="C4-dicarboxylate transport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3860.10">
        <property type="entry name" value="Sodium:dicarboxylate symporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001991">
        <property type="entry name" value="Na-dicarboxylate_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018107">
        <property type="entry name" value="Na-dicarboxylate_symporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036458">
        <property type="entry name" value="Na:dicarbo_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42865">
        <property type="entry name" value="PROTON/GLUTAMATE-ASPARTATE SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42865:SF7">
        <property type="entry name" value="PROTON_GLUTAMATE-ASPARTATE SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00375">
        <property type="entry name" value="SDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00173">
        <property type="entry name" value="EDTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118215">
        <property type="entry name" value="Proton glutamate symport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00713">
        <property type="entry name" value="NA_DICARBOXYL_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="190"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="228"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="315"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="339"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="427" mass="45018" checksum="F387A39D5275EBF0" modified="2017-09-27" version="1">MGLLRKYLDYPVLWKILWGLILGAIVGLALGKAGYADAVKVYIKPFGDLFVRLLKMLVMPIVLASLVVGAASISPARLGRVGVKIVLYYLLTSAFAVFFGLLMGNLFRVGTGISLGTGEGKAIEATRPSLVTTLLNIVPTNPFASLSNGEVLPVIFFAIILGIALTYLINRDDERVRTAGTTLLRAFDGLAEAMYLIVAGVMQYAPIGVFALIAYVMAEQGTKVVGPLAKVVVAVYIGLILQIVLVYFVLLKVFGIDPLKFLKKAKDAMLTAFVTRSSSGALPVTMRVAEEMGIDRGIFSFTLPLGATINMDGTALYQGVTVLFVANAIGYPLTLGQQLTVVLTAVLASIGTAGVPGAGAIMLAMVLQSVGLDLTAGSPVALAYAMILGIDAILDMGRTMVNVTGDLTGTTIVAKTEGELDESAWKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P984</accession>
    <name>A0A218P984_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">fructose-bisphosphate aldolase</fullName>
            <ecNumber evidence="4">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_08420</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DeoC/FbaB aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.13" evidence="4"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854591.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P984"/>
    <dbReference type="GeneID" id="33316290"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08420"/>
    <dbReference type="OrthoDB" id="6329at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00958">
        <property type="entry name" value="DhnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050456">
        <property type="entry name" value="DeoC/FbaB_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053414">
        <property type="entry name" value="FBA_class_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041720">
        <property type="entry name" value="FbaB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040816">
        <property type="entry name" value="Fbpase1_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005556">
        <property type="entry name" value="PRK07226.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47916:SF1">
        <property type="entry name" value="3-HYDROXY-5-PHOSPHONOOXYPENTANE-2,4-DIONE THIOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47916">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE CLASS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01791">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038992">
        <property type="entry name" value="Aldolase_Ia"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01133">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with dihydroxyacetone-P" evidence="9">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR038992-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ07344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="281" mass="31343" checksum="EAEB6813A6344332" modified="2017-09-27" version="1">MDAYQSVGIRRRMKRFFRRDGRALIFAMDHGFEHGPTDFEEHWEHVDPRIIMRKVVRAGIDGVMMLPGVARLAVDELKPDTGLMIKLTSKTELRPKDDQLLQSQLGFVEDAVKLGADAIAATVYWGSPQEDVMMRQFAEIASYAHDLGFPVVQFAYPRGPYINERYGKKEDYRVVMYGARAAVESGADMIKTYWTGSKETFAKVVEAAAGVPVLLSGGAKTDNPVDFLKLVWEVIEAGGAGAVVGRNIFQRENPEPFIKALLKVVHRNEDPEEAAKAEGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P985</accession>
    <name>A0A218P985_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Rubrerythrin family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_08340</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Encapsulin nanocompartment</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854575.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P985"/>
    <dbReference type="GeneID" id="33316274"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08340"/>
    <dbReference type="OrthoDB" id="60579at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140737">
        <property type="term" value="C:encapsulin nanocompartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051429">
        <property type="entry name" value="Encapsulin_nc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rr_diiron-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37165">
        <property type="entry name" value="PEPTIDASE U56 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37165:SF1">
        <property type="entry name" value="TYPE 1 ENCAPSULIN SHELL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1284">Encapsulin nanocompartment</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rubrerythrin diiron-binding" evidence="3">
        <location>
            <begin position="24"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00033738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00033787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="100" mass="11600" checksum="B4351E99454C8B1F" modified="2017-09-27" version="1">MLAKYPFELPKDRPLTKTEIAQALRWAIEAELDAINIYEQLAAGIEDERIKHIFLDVANEEKEHFGEFLAALFEVDEELAKYMKKGFEEVEEETGIKVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9C8</accession>
    <name>A0A218P9C8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854587.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9C8"/>
    <dbReference type="GeneID" id="33316286"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08400"/>
    <dbReference type="OrthoDB" id="94709at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="469"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="489" mass="54660" checksum="DD173E0AEBBD275E" modified="2017-09-27" version="1">MKKVLAAIFAAILLTSFVGPGFVSAGDEVQVYKQGFTLKIALMPNGSANITMTSVLLGPKEAIDKQRETILNETQNGNMTIEEAIKKFEQEQLARYIQGLTQAGVKLVNESMKSYGIEEGNNITLVFNAIALDYAKYYSYDHHWEVWVDPTRGYASMMVPDTGFPFGIESNNTFIVVLPPNATLLSYPKPFVKQYNQSRFVVGSSVEGNTVVVRSHIYIEPWLTPDGYKTLFGDYNDYYIRYETPYEGVEHYDKSVTNEYVTLNIYSNGTVRLHMKDEYVEPLRDVITRKAEIVSYGVKNVTEYILRTYSLALGYQGAIVDGGKVTILGLNETTAPLVIDAEYTLRNFTKYENGSYVYTFDPTLGMANGLTSRVEYEVNNTLYLTLNLSEGGDFLEIPSNISREVKGNRFTMTVVRNGNILRITSNVYLRYGAQPEDVKELLANYTSATVRYTLPAEGTGGGMSSTQKVIATVVAALLIVGAIAFWKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9F5</accession>
    <name>A0A218P9F5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dihydropteroate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854644.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9F5"/>
    <dbReference type="GeneID" id="33316354"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08740"/>
    <dbReference type="OrthoDB" id="70327at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042558">
        <property type="term" value="P:pteridine-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="entry name" value="Dihydropteroate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005236">
        <property type="entry name" value="Dihydropt_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045406">
        <property type="entry name" value="DUF6513"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025595">
        <property type="entry name" value="PterinBD-DUF4346"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00284">
        <property type="entry name" value="dihydropteroate synthase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20123">
        <property type="entry name" value="DUF6513"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14251">
        <property type="entry name" value="PterinBD-DUF4346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="Dihydropteroate synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pterin-binding" evidence="1">
        <location>
            <begin position="145"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="515" mass="57875" checksum="F70F35499C41B93F" modified="2017-09-27" version="1">MENAGRILLVTGRLAEPLVRKYGKGCDVFVTPVSVAAFLTPELIARYLKKAGIKSEDYGLVLIPGLIRGSAQIIEDELGIPAFKGPRNAMDLPQVLKALSEGFRLSREKPAEDLFSVDALKKVEDIRNKTRNRRYIEDVLKKSWNILIGDLPAGRDFPARILGEVVDAPRLGVEKTVEKALYYLREGADIIDIGMVAGETNLDFIEEIPEIREQLKDNGFDVPISFDSLNTAEIEKALDYADLFLSVDEGNLEALVTEKPVVLIPTNQKEGFFPTKPSERVEFLERLKERAFDLGYKTVIPDLILEHVPHLARSITAFQLYRERNPDDVLLAGVGNVVELYDADSVGMNALLAGIAKELSISLLLTTEVSAKAKGSVRELRRAVDMNLFDMPKDLGFDLLLLKEKRASEWRFEPAEKIIEAGERPVQLEPVYFRVWVENGKIWVNAHRGTEAVLTITGDEPNAIIDTILEHFEISPRHAFYLGRELERAKTALKLRRSYVQEVELFGEFYVGQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9H5</accession>
    <name>A0A218P9H5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_08930</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Probably part of an ABC transporter complex. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854679.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9H5"/>
    <dbReference type="GeneID" id="33316392"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08930"/>
    <dbReference type="OrthoDB" id="35850at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000224">
        <property type="entry name" value="Energy-coupling factor transporter ATP-binding protein EcfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050095">
        <property type="entry name" value="ECF_ABC_transporter_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43553:SF25">
        <property type="entry name" value="ABC-TYPE COBALT TRANSPORT SYSTEM, ATPASE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43553">
        <property type="entry name" value="HEAVY METAL TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="2"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ07434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="263" mass="29364" checksum="890E499903719218" modified="2017-09-27" version="1">MIRVKNLWHVYENGREALKGVDFEMGSEIVALVGQNGSGKTTFAKHLNGLLKPTKGTVEVDGLDTREHTVAELSRLVGYVFQNPEHMFFEESVFREVAFGPRNLGLSEGEIEERVRWALRAVNLEGYEERTPYSLSGGEKQRLAIACVLAMRPKYLILDEPTTGLDGRSSASVVEAIRKLHSEGHGILLITHDMELVLELAERVVLLHRGEKAFDGPVEEFFSLELHDYGLEKPELLRISEKVGVGFVKSVPELVEVLVGGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9K3</accession>
    <name>A0A218P9K3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="11">DEAD/DEAH box helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_09070</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854703.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9K3"/>
    <dbReference type="GeneID" id="33316420"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09070"/>
    <dbReference type="OrthoDB" id="11644at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12089">
        <property type="entry name" value="Hef_ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20075">
        <property type="entry name" value="XPF_nuclease_XPF_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001992">
        <property type="entry name" value="ATP-dependent RNA helicase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1320.20">
        <property type="entry name" value="hef helicase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041663">
        <property type="entry name" value="DisA/LigA_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041755">
        <property type="entry name" value="Hef_ID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010337">
        <property type="entry name" value="PRK13766.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14025">
        <property type="entry name" value="FANCONI ANEMIA GROUP M FANCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14025:SF20">
        <property type="entry name" value="FANCONI ANEMIA GROUP M PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12826">
        <property type="entry name" value="HHH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21210">
        <property type="entry name" value="RNA_helicase_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="18"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="342"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="509"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="509"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="536"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ07459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="794" mass="90799" checksum="A16333229C49D57B" modified="2017-09-27" version="1">MPYLRRDIIEPRVYQEVIYARCKETNCLVVLPTGLGKTLIAMLIADYRLSTYGGKVLMLAPTKPLAVQHAESFKRLFNLSPEQINVLTGELPPNKRQEIWEKSTIITATPQTVENDVITGKISLEDVVLLVIDEAHRAVGGYSYVFIAKEYLKTARHPLVLGLTASPGSDEEKIREIVENLGIEHIEVRTESSPDVKPYVQRIAFEWVKVELPEIYKEVRSLLREMLKESLKPLAQFKLVSTYSPDIPKREVLQAGSRINREVAQGNYELGRLRVYQAKAVKLQHAIELLETQGLTALRAYLKKLREDKRTKSSRQLMEDPRMRKVIYLLVQAKELGIDHPKMERLLELVGKQLGRKPDSKIIVFTNYRDTGKKIVEELTKRGISSERFIGQASRGEDKGMSQKKQKETLERFSRAEFNVLVATSVGEEGLDVPEVDLVVFYEPVPSAIRSIQRRGRTGRHRPGRVVILMAKGTRDEAYYWSSKRKEKGMFDAIKRIARELEREKPKRAEIREKVPERAEMSRGKVTSLDAFLKPKKSEEKKSEKVEEDSKAEKVEEPKKMEKDSVEDLPIKPIFVRKPKGIVVYVDNRELRSGVPKHLKELGADVEVRTLDVADYVVSEDVGIERKSANDFIQSIIDGRLFDQVERLKRAYEKPVIIIEGELYGIRNVHPNAIRGAITAVTLDWGVPILFSSGVKETAQFIYLMAKREQEERKKEVRLRSEKKALTLAERQRLIVEGLPNVSATLAKRLLKHFGNVERVFTATEEELKEVEGIGPKKAREIRRVITAPYVEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Q7</accession>
    <name>A0A218P9Q7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Potassium channel protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854747.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Q7"/>
    <dbReference type="GeneID" id="33316471"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09325"/>
    <dbReference type="OrthoDB" id="85913at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="entry name" value="Phosphate transport system protein phou homolog 2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="entry name" value="Regulator of K+ conductance, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050144">
        <property type="entry name" value="AAE_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30445">
        <property type="entry name" value="K(+)_H(+) ANTIPORTER SUBUNIT KHTT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30445:SF8">
        <property type="entry name" value="K(+)_H(+) ANTIPORTER SUBUNIT KHTT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109755">
        <property type="entry name" value="PhoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="TrkA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0407">Ion channel</keyword>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RCK C-terminal" evidence="1">
        <location>
            <begin position="109"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="201" mass="22764" checksum="54C044A387CAA27C" modified="2017-09-27" version="1">MEEWNEVEVPKNVKDIFIEMKNTAELMVDLAYSAALFKEKEMAEEVLELEEYLDILNYHLMVRAVLAARNMKEAEQITSILQMARSIDDISNAAADLAKMVLEEKIHPLITEVILESEETIGKIVVSPNSTLVGKSLEELDLSTNTGVWVIAIRRGKRWIFDPDEDTKIMPGDIIIGRGTRTALDYLREIARGVIKVMSNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9R1</accession>
    <name>A0A218P9R1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">AAA family ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_09425</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family. CDC48 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854765.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9R1"/>
    <dbReference type="GeneID" id="33316491"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09425"/>
    <dbReference type="OrthoDB" id="77269at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19511">
        <property type="entry name" value="RecA-like_CDC48_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000007">
        <property type="entry name" value="AAA family ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.10:FF:000005">
        <property type="entry name" value="AAA family ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000057">
        <property type="entry name" value="AAA family ATPase, CDC48 subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000018">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005938">
        <property type="entry name" value="AAA_ATPase_CDC48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01243">
        <property type="entry name" value="CDC48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="7">
        <location>
            <begin position="14"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48" evidence="6">
        <location>
            <begin position="116"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="244"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="579"/>
            <end position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="837" mass="94073" checksum="6DA48E663A51A4BA" modified="2017-09-27" version="1">MIFGKDEERYEKIKLRVAEALKRDVGRGIVRFDRRYQKQLGVEPGDIVELIGERSTAAIVANPHPDDRGLDIVRMDGYIRRNAGVSIGDYVTIAKAEVQEAKKVTLAPAQKGVFIQIPGEMVKGNLLGRPVVKGDLVVASSRNETYYGGSPFDELLRGLFETMPLGFGELKFVVVNTVPKGIVQITYNTEVEVLPQAVEVREEAIPEVTYEDIGGLSDAIQKIREMVELPLKHPELFERLGIEPPKGVLLYGPPGTGKTLLAKAVANEANAHFIAINGPEIMSKFYGESEERLREIFKEAEENAPAIIFIDEIDAIAPKREEVVGEVEKRVVSQLLTLMDGLKGRGKVIVIAATNRPDALDPALRRPGRFDREIEVGVPDKAGRKEILQIHTRGMPLEPDYDKETVLRVLRGLMKRKTFDEGTLKRVIERVEDARSEDEVKEALKSESEVYAEVRTRLIDRMLDEIADKTHGFVGADLAALAREAAMVVLRRLINEGKVSPEQERIPPEVLQELRVRKADFYEALKMVEPSALREVLLEVPNVHWDDVGGLEEVKQELREAVEWPLKYPKAFQRLGIEPPRGILLYGPPGTGKTLLAKAVATESEANFIGIRGPEVLSKWVGESEKRVREIFRKARQAAPTVVFIDEIDAIAPARGMEGDRVTDRLINQLLTEMDGIERNSGVVVIAATNRPDILDPALLRPGRFDRLILVPAPDEKARLEILKVHTRRVPLAEDVNLRELAKRTEGYSGADIEALVREAALIAMRRIMRELPEELVEEESEEFLEKLKVSRKDFEEALKKVRPSITPYMVEYYKNFEENRRRSGEKGAKGPDYYTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9S0</accession>
    <name>A0A218P9S0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Putative asparagine synthetase [glutamine-hydrolyzing]</fullName>
            <ecNumber evidence="8">6.3.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_09375</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>L-aspartate + L-glutamine + ATP + H2O = L-asparagine + L-glutamate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12228"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.4" evidence="8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854757.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9S0"/>
    <dbReference type="GeneID" id="33316481"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09375"/>
    <dbReference type="OrthoDB" id="8692at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00352">
        <property type="entry name" value="Gn_AT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050795">
        <property type="entry name" value="Asn_Synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01536">
        <property type="entry name" value="asn_synth_AEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772">
        <property type="entry name" value="ASPARAGINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772:SF2">
        <property type="entry name" value="ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001589">
        <property type="entry name" value="Asn_synthetase_glu-h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="4 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="10">
        <location>
            <begin position="2"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="345"/>
            <end position="346"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR001589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ07513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="480" mass="52797" checksum="3FADC7B6DDAA029F" modified="2017-09-27" version="1">MCLVAGGIGSNLREKFILMINAGKHRGEDSFGVWTDAGVLKSDDFSRVGEIPEGRIGLIQCRLAMTGSKTFMQPFVNDFALVHNGEIYNHRELRAWLEGRGVSFESDVDSEVILRLIEYLLGSGLSIHDAVRKAMTMLEGDYAVAISDGKVIHLFRDPVGIRPLYYSPRGFFASEKKVLWAIGEEAIPVRPGELVTISGSGAAMRRVFSLDELKRSPLSGERAVNAVLKTLACSVRHRVGKKTGVLFSGGLDSSLVTLLASEYSDVVIYTAGAEGSPDLEWARMAADKLGLPLKEYVFDVEDVRDAVPKVVFAIEEPNPMNLAIGIPLYFATRLAREDGTKVLLSGQGADELFGGYAKYLERPELMELDLRELSERNLARDDKIAMLNSVEGRFPFLSLPVISAALNTPLEAKISGGVRKAVLRRAAIRAGLPEEIAMREKKAAQYGSHAQKLLEKVAKGEGLSLREYAEKAFREMFKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9V7</accession>
    <name>A0A218P9V7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_09680</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854813.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9V7"/>
    <dbReference type="GeneID" id="33316542"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09680"/>
    <dbReference type="OrthoDB" id="101799at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="148"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="14"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="160"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="172" mass="19274" checksum="D5695273585C7F4D" modified="2017-09-27" version="1">MANVEEVEIEKKAPAQLEKLLEELVEEMKNAKSEEELKLLEKKVDIIEDLIEAYYGDRDIEQAAKLMDKVGPMIEDILEPLKNLLSELYSPEKMQAMGKSVAEFYKNLVEAGMDKETATELTKEYMKSINAPKALLEALTGLMKGKGGSSGPINITLGKARPENAKEERKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA04</accession>
    <name>A0A218PA04_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ArsR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09685</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854814.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA04"/>
    <dbReference type="GeneID" id="33316543"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09685"/>
    <dbReference type="OrthoDB" id="92467at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="146" mass="16780" checksum="680934D3C452123E" modified="2017-09-27" version="1">MPDEDLAREVQELKKALEAMRASFEVVSKMAQAYLRLLNVYAEYGGLSIDVVIPEIKHDPIAREIVKILFDLKRANVSQIARELKGRRGKASRNTVRAKLEELERLGVVREVPAERGKVYALSRDVVRKWLELIGMPIRFDQTNDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA33</accession>
    <name>A0A218PA33_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glucose-1-phosphate thymidylyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_03035</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854875.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA33"/>
    <dbReference type="GeneID" id="33315213"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03035"/>
    <dbReference type="OrthoDB" id="15372at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04189">
        <property type="entry name" value="G1P_TT_long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005908">
        <property type="entry name" value="G1P_thy_trans_l"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01208">
        <property type="entry name" value="rmlA_long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42883">
        <property type="entry name" value="GLUCOSE-1-PHOSPHATE THYMIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42883:SF2">
        <property type="entry name" value="THYMIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="2">
        <location>
            <begin position="268"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="352" mass="39408" checksum="063110AB76D34505" modified="2017-09-27" version="1">MKALILSGGHGTRLRPLTYSQQKQLIPVANKPVLFYAIEDVIEAGIHEIGIIVGPNAEQVKETVLSREWDAEIGFIYQDDPKGLAHAILVARDYLGDDDFVMYLGDNILREGIVEHLKHFKEGNFDASILLQEVLNPQQFSVAELSEDGKTIKRLVEKPKVPPSNLALVGIYFFKPIIHEAVRNIKPSWRNELEITDAIQWLIDHGYRVGWTKVTGWWKDTGKPEDLLDANRLILDDIKTDIRIHTNARIHGRVVIEEGSEIDKNTVIKGPVVIGKNVKIKNSYIGPYTSIGDNVVIENTEIEDSIILEGSEIRNAGRIVESLIGRGVKIINGTSHPFGRKLVIGDNSRLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA44</accession>
    <name>A0A218PA44_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05985</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA44"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05985"/>
    <dbReference type="OrthoDB" id="91341at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="365" mass="41943" checksum="9E98EA31854AEFBE" modified="2017-09-27" version="1">MPYFRNLASVNIQNAGEAHVFVSALYPEGFRQIAEYDIKNSGRITLNLGDLRKAWDDEYRKNKRLSQPLILLTIVTKDGNVSIESVGFEWDRMPQSWVVTPKFRTLKNAVKTSSKQTNDVHPLYYYSRTLVDSYEWTAPTIVAQVIPDSSTKGYISYTYFINKKVGFSINAFVDTDWTRLTSYNIIQKDESGKHASGFSVGSPYYIWMNVTYRYERWRVYSAGWVWYEEYVYVKDFYPSTISGGTSKPSNAQVPPIDRWIDEGVYESTGDIGYYAFHIWDLDTSDFAMDVLKFIDVLELMGKISETAAQKATLIGLFIDVGFEYDNMKAFDFDLSLDSGVQTSHHVWRGESTDISTVPVLYFNVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA62</accession>
    <name>A0A218PA62_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Sodium transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_06920</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA62"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06920"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046942">
        <property type="term" value="P:carboxylic acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10322">
        <property type="entry name" value="SLC5sbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018212">
        <property type="entry name" value="Na/solute_symporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050277">
        <property type="entry name" value="Sodium:Solute_Symporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48086:SF7">
        <property type="entry name" value="SODIUM-SOLUTE SYMPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48086">
        <property type="entry name" value="SODIUM/PROLINE SYMPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00457">
        <property type="entry name" value="NA_SOLUT_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="128"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="173"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="210"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="297"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="326"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ07668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="387" mass="40302" checksum="13BD228C842D89BD" modified="2017-09-27" version="1">MRVLARYTVPEVLEMRYKSKAIRLLAAGLSLLALVGILGAQVWAASAVFEAIGLPGTAGAVFATLVFIAYTALSGLWAVALTDFIQIIIGSIGIFVAVVLGLVKVGGISGLKTALDGMSGLPQPPSEYFSLTSLGLSLLALTLIATVMYTLIGQDFYQRLFAAKDERTARKGAVYAGVLLMALSVLPALAGMLAVALSGNPEAVIDSPKIAVPMLVISVFDGTVGAIFVAAVLAAIMSTADSLLSAATSHIVKDFYEGIVGEKSDEKKLLRLSVLTTVAVGLLSLGAALAIQGIVELLIYSYDVYTSGVFVPLVLGLYWKRATKEGALAGMVAGSLVAILGAAGVLNFPYWEYIYVSGALVSAIVMVIVSVLTEVEEIDEELKAAFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA86</accession>
    <name>A0A218PA86_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_08595</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA86"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08595"/>
    <dbReference type="OrthoDB" id="102547at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="95" mass="10556" checksum="552A3021C8B09E6E" modified="2017-09-27" version="1">MIVRGRIILKGHSVPFEVSKTGKGLSLRIELPKKPLEYSVDCPCLEPLELLGLFLPALEEELGEVEGVFVEEVVGEGVGGKLTERLRKLFNPQER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA89</accession>
    <name>A0A218PA89_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sulfhydrogenase 1 subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_06480</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854904.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA89"/>
    <dbReference type="GeneID" id="33315899"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06480"/>
    <dbReference type="OrthoDB" id="42371at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="entry name" value="Cytochrome-c3 Hydrogenase, chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018194">
        <property type="entry name" value="Ni-dep_hyd_lsu_Ni_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054945">
        <property type="entry name" value="sulfhyd_HydA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040832">
        <property type="entry name" value="sulfhyd_HydA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43600">
        <property type="entry name" value="COENZYME F420 HYDROGENASE, SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43600:SF4">
        <property type="entry name" value="CYTOSOLIC NIFE-HYDROGENASE, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="HydB/Nqo4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00508">
        <property type="entry name" value="NI_HGENASE_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0533">Nickel</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="418"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="421"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="424"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601501-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="428" mass="48203" checksum="5B90B4B9E0B7A01F" modified="2017-09-27" version="1">MKNLYLPITIDHIARIEGKGGVEIVVGDDGVKEVKLNIIEGPRFFEAISLGKKVDEALAIYPRICSFCSAAHKLTAVEAAEKAVGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDYLGYSGPLHMIEEYKKEIGIALDLKNLGSWMMDELGSRAIHQENVVMGGFGKLPDKSVLETMKKRLKEALPKAEYTFELFTKLEQYEEVEGLITHIAVKPRGDVYGIYGDYLKASDGNEFPSEEYKEHIKEFVVEHSFAKHSHYHGKPFMVGAISRLVNNAGTLYGKAKELYEAHKDLLRATNPFANNLAQALELVYFTERAIDLIDEVLAKWPVKPRDEVEIRDGFGVSTTEAPRGVLVYALKVENGRVSYADIITPTAFNLAMMEQHVRMMAEKHYNDDPERLKLLAEMVVRAYDPCLSCSVHVARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA91</accession>
    <name>A0A218PA91_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Beta-galactosidase trimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_08315</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA91"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08315"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03143">
        <property type="entry name" value="A4_beta-galactosidase_middle_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013738">
        <property type="entry name" value="Beta_galactosidase_Trimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08532">
        <property type="entry name" value="Glyco_hydro_42M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-galactosidase trimerisation" evidence="2">
        <location>
            <begin position="81"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="424" mass="47913" checksum="474160DBE126E2C7" modified="2017-09-27" version="1">MSSGLVSSNTPQNQSYRILFVCSDLEDVTLANSFGNNGDILYLGKDLPDDRSPLSDILYLSKYDEIWIPDLNVQWTEGGRLSEGEIGALTEYVQSGGVLVIGLNTYTQSWSRKLDALLGVRVLRLESPIEDGEDLDIVFNERVYPYNDTYQAVIIRPEGAKVIARYRNGNPAITLNSYGSGVAVLMTFNPVKAYVEGNPSFVDLYREVSSEALKERKNPPTISNTKKTLILLKRVILHPITLGLLVFILLEILAYLGLMPLGLTVFFAAPFIPFWGIIKRRKRCTSVLEIISMMRGVTLSELSNEMGEKVRRLKFCIAILKLKRKISILDISRLGGRDYLITLHGLESEGVAAWAVEMYPRLMERIALHPGVTILDLARSSNMPPYEVLQLLREMSRYGVVEIRKMPFDYEVYSTRALLRWFEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA92</accession>
    <name>A0A218PA92_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_09575</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA92"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09575"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00748">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81593">
        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="2">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="125" mass="14883" checksum="D2097603B6A76965" modified="2017-09-27" version="1">MELANSAFESEKYDAAIFLAEQALQLHIKALLIKYADLRIRSHSLRELLYRLGQVFKLEDRIEEFIRANRKLLRELEDAYIETRYEARVYYREDAEELIEFVARVMDFVEGLADEFERGSNLQDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA95</accession>
    <name>A0A218PA95_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mut7-C RNAse domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854944.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA95"/>
    <dbReference type="GeneID" id="33316520"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09570"/>
    <dbReference type="OrthoDB" id="1266at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002782">
        <property type="entry name" value="Mut7-C_RNAse_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39081">
        <property type="entry name" value="MUT7-C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39081:SF1">
        <property type="entry name" value="MUT7-C RNASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01927">
        <property type="entry name" value="Mut7-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mut7-C RNAse" evidence="1">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="154" mass="18020" checksum="D39F48BA594812D2" modified="2017-09-27" version="1">MRFIADMMLGRLARWLRLYGYDTLYGIEDDDEIVHVALKEDRIILTRDSGLAERAKRLGAKVFLLSSNSLEEQVKELKRLGVDFRELFPPSARCPKCNGPIGRASKEEVRGKVPSTVYERYDEFYICQNCGQIYWPGRQWREMLKIDKKLRGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P4X5</accession>
    <name>A0A218P4X5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF304 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853095.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P4X5"/>
    <dbReference type="GeneID" id="33314610"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00035"/>
    <dbReference type="OrthoDB" id="86211at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="145" mass="16167" checksum="C3F0C8B0DF011EFE" modified="2017-09-27" version="1">MALYEENVSSRGFRIFLILAALPMLFGLYATYMAGEGFEFMLITALLVVLVLVDAMELRIEIDEREVRIRGPIGLLIKKTVKIENIESFSVAEGWMSCSGAIHFNLPAKGCIMLRQKKGWTVSFTTNNPEEVARVLAMLGVPRAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P503</accession>
    <name>A0A218P503_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00205</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853129.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P503"/>
    <dbReference type="GeneID" id="33314645"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00205"/>
    <dbReference type="OrthoDB" id="101641at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="109" mass="11819" checksum="05A143070437408F" modified="2017-09-27" version="1">MRPKPAIVSFFLLLSLFFYGIGLLGGDLSDIAGYGVIGTIHLIFAASIYRGHETIVDISPYIALLDMLFGLLWIMVGLSLPAFTLTLLSALILVALMDEDVRTELKMGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P510</accession>
    <name>A0A218P510_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF2178 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853131.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P510"/>
    <dbReference type="GeneID" id="33314648"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00220"/>
    <dbReference type="OrthoDB" id="98535at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019235">
        <property type="entry name" value="DUF2178_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09946">
        <property type="entry name" value="DUF2178"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="127" mass="14161" checksum="14338C31DBCDF087" modified="2017-09-27" version="1">MKAKYWFGWGIMFVALIGLFIFASNAGSVLLGVSSLLLGAIVATAYQRYLESIGEMITDERLRLIEEKSYALTMRVLGFTIALTFVSTSIISQSNPFWKSASIVSGLFMFVYALLPIFAKAHYERVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P521</accession>
    <name>A0A218P521_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF2178 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_157721563.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P521"/>
    <dbReference type="GeneID" id="33314647"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00215"/>
    <dbReference type="OrthoDB" id="101256at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019235">
        <property type="entry name" value="DUF2178_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09946">
        <property type="entry name" value="DUF2178"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="13984" checksum="9CB6305730AEBA8C" modified="2017-09-27" version="1">MERWKLIGYVIPATTASLLAVALWMGNAPLAFGVLAGAIGVMLLYSEWLRGRGEVLSDERTLRIEEMASKRTLQVLILVIAFLVVGLSIFSERDPGLRSAYYLSLGLMVLISALKVSLKRHYSRVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5A1</accession>
    <name>A0A218P5A1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_00605</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C56 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853202.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5A1"/>
    <dbReference type="GeneID" id="33314725"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00605"/>
    <dbReference type="OrthoDB" id="82036at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd03134">
        <property type="entry name" value="GATase1_PfpI_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006286">
        <property type="entry name" value="C56_PfpI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053435">
        <property type="entry name" value="Peptidase_C56_Deglycase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040823">
        <property type="entry name" value="deglyc_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01382">
        <property type="entry name" value="PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42733">
        <property type="entry name" value="DJ-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42733:SF2">
        <property type="entry name" value="DJ-1_THIJ_PFPI FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51276">
        <property type="entry name" value="PEPTIDASE_C56_PFPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DJ-1/PfpI" evidence="2">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ05940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="166" mass="18475" checksum="9F8BEB768E095DFD" modified="2017-09-27" version="1">MKVLFLSADGFEDLELIYPLHRIKEEGHEVYVVSFQRGKITGKHGYSVNVDLAFDEVDPDEFDALVLPGGRAPEIVRLNEKAVSIVKKMFEDGKPVASICHGPQILISAGVLKGRKGTSVITIRDDVKNAGAEWVDAEVVVDGNWVSSRHPGDLYAWMREFVKLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5E7</accession>
    <name>A0A218P5E7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable ribonuclease FAU-1</fullName>
            <ecNumber evidence="6">3.1.26.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">RNA-binding protein FAU-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">fau-1</name>
        <name evidence="8" type="ORF">A3L08_00950</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Probable RNase involved in rRNA stability through maturation and/or degradation of precursor rRNAs. Binds to RNA in loop regions with AU-rich sequences.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the FAU-1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.-" evidence="6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853260.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5E7"/>
    <dbReference type="GeneID" id="33314794"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00950"/>
    <dbReference type="OrthoDB" id="84798at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016891">
        <property type="term" value="F:RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.380.10">
        <property type="entry name" value="FomD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01910">
        <property type="entry name" value="RNA_binding_AU_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007295">
        <property type="entry name" value="DUF402"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035930">
        <property type="entry name" value="FomD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050212">
        <property type="entry name" value="Ntdp-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019307">
        <property type="entry name" value="RNA-bd_AU-1/RNase_E/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016730">
        <property type="entry name" value="RNA-bd_FAU-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39159">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39159:SF1">
        <property type="entry name" value="UPF0374 PROTEIN YGAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04167">
        <property type="entry name" value="DUF402"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10150">
        <property type="entry name" value="RNase_E_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018644">
        <property type="entry name" value="RNA-binding_FAU-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159234">
        <property type="entry name" value="FomD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 6" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="S1 motif" evidence="7">
        <location>
            <begin position="92"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ05998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="471" mass="54222" checksum="3CB7CDAFC1E919B2" modified="2017-09-27" version="1">MSTDTGVTVRIRGIYSTALTKLFLDRGFGIAQPSNKIVERLGLEKTYDEFDVDIYDKKDHHGVVLVGTKVEEVKEVLEEEFIDVFFRKLPYQLYGVYKGIVVQRDEKYVYVDIGSAIGTIPVRDLPRAKEGDELLVQVKKHNLLPQLSVMITVPGDYAVLIPKPVGAQRHVKISRKIRDSQERERLRILGLSIDLGEWGILWRTAAAYKDWNILRDEIIKLSKLSDRLAKANSYTAPSLIIEGRNIYEVEFGGGAKRKLDEIRNKAVPTVEGHHQLKAYDLEFSFAVEIAEGILAKVPGQRAKVNQGFWEALIENKGPRKGWLFRLEHHKPDGQLIKIGPGEIQEVSTNPLKVTFRRHLKPGKFYDGLDIPIEFGDYVITEIEAGKWWFVHRYYDKNGNLKGEYYNINTPVEIYPDRARYIDLEVDIVKWPDGKKEIIDKEKLTEHYEEGTISEKLYRAVLRIVQEVYDRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5G0</accession>
    <name>A0A218P5G0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_01060</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853281.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5G0"/>
    <dbReference type="GeneID" id="33314816"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01060"/>
    <dbReference type="OrthoDB" id="102102at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF137">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="5"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="138" mass="14546" checksum="9FD7C117ED35309D" modified="2017-09-27" version="1">MREYIIYAVLAAFFASLVPIFGKLGLKDVDPTLATAVRAVIMAVFLVGVAFLSGSTNVSEIDGRALLLITLSGLAGALSWLFYFMAIKNGRVPSVIAIDKTSVALAIFLSWLVLGSKMDLKTALGALLIVIGAILVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5I5</accession>
    <name>A0A218P5I5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Universal stress protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_01200</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853305.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5I5"/>
    <dbReference type="GeneID" id="33314844"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01200"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF25">
        <property type="entry name" value="USPA DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="2"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="149" mass="16744" checksum="1E20EE0EEF574740" modified="2017-09-27" version="1">MRILVLIDGSKWSQKAALHAIAIAKRKRGKVILFSVLDRREAKAMAFNMGLISENLKDIQRFEETIWKDMKKGIRDIMSNLLEFCQEQGVNCSFRIVEGSARDKILEEANSGDYSLVVMGAYGRSGKTRIGSLLEEVVGLIEPPVMVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5I7</accession>
    <name>A0A218P5I7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853301.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5I7"/>
    <dbReference type="GeneID" id="33314840"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01180"/>
    <dbReference type="OrthoDB" id="97284at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="196" mass="21260" checksum="357AB41B083116EF" modified="2017-09-27" version="1">MRRTVLLALTIALMLPVSGCIGGPAGTATEPTESRNIHPGYGEFNAGNVLIGDGEVMMDHYGLVIPVNRTAILKGIIFAREYRVSSGNGSDTTGYYGGKVELKAYLADIFVQYAWKALESGLKPVSELEVEITPETVEVKPGKNEAFEVKIDTSEVTLGKTYYLYIVAFGGDGWKGWAVVEVITEETTVTKETEND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5J3</accession>
    <name>A0A218P5J3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Deacylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_01220</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853309.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5J3"/>
    <dbReference type="GeneID" id="33314848"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01220"/>
    <dbReference type="OrthoDB" id="24854at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08659">
        <property type="entry name" value="M20_ArgE_DapE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050072">
        <property type="entry name" value="Peptidase_M20A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808">
        <property type="entry name" value="ACETYLORNITHINE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808:SF8">
        <property type="entry name" value="PEPTIDASE M20 DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00759">
        <property type="entry name" value="ARGE_DAPE_CPG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="6">
        <location>
            <begin position="164"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="368" mass="41151" checksum="028640619E732AC4" modified="2017-09-27" version="1">MEFDLLKELVSIDSPFGHEEEISRFIASFLEEHGFDVELLPVEGFGDDVIAYLPGRGYTVVLNGHMDTVRPSPGWTRNPKGELDGDRFYGLGSADMKGGLAALMSAFVELAELPKRERPNVIFTAVSDEEGYSRGAWELIKSGKLENADLVLVAEPTNETLMLGARGRFVVQVEVFGRKAHAARPHMGINAIEELGRFLGSLGRIRFRSHRKLGIGSYCTLHIEGSADGLSVPDYARAIIDRHVVPGEEWERVEKELIELAERIKLKGELKVTRLGRPTPDMLPYTVRENNRFANLFKSAHRSVLGREPEVTYGKSVGDFNYFGTYLGKPTLVYGPVGGSWHSPNEWVSVESVRRVKRVYIEFLKSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5J8</accession>
    <name>A0A218P5J8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fumarate hydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_01235</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I fumarase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853311.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5J8"/>
    <dbReference type="GeneID" id="33314851"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01235"/>
    <dbReference type="OrthoDB" id="34134at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.130.10">
        <property type="entry name" value="Fe-S hydro-lyase, tartrate dehydratase beta-type, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004647">
        <property type="entry name" value="Fe-S_hydro-lyase_TtdB-typ_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036660">
        <property type="entry name" value="Fe-S_hydroAse_TtdB_cat_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006253">
        <property type="entry name" value="PRK08395.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00723">
        <property type="entry name" value="ttdB_fumA_fumB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43351">
        <property type="entry name" value="L(+)-TARTRATE DEHYDRATASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43351:SF2">
        <property type="entry name" value="L(+)-TARTRATE DEHYDRATASE SUBUNIT BETA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05683">
        <property type="entry name" value="Fumerase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117457">
        <property type="entry name" value="FumA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe-S hydro-lyase tartrate dehydratase beta-type catalytic" evidence="3">
        <location>
            <begin position="2"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="171" mass="18713" checksum="A6AB66D2D63A58BC" modified="2017-09-27" version="1">MAVKLRTPLSREDVLKLRAGDIVHLSGEIVTARDSAHRRILSLPREELPFNPEGAVIYHCGPVVRKKPDGWEIVSAGPTTSSRMNRYLDRILGLGVRGIIGKGGMNPEPFRGRAVYLAFTGGAGSLAAESVKRVKAVHWLDLGVPEALWVLEVRDFPLIVAIDAHGESLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5L3</accession>
    <name>A0A218P5L3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">2-ketoisovalerate ferredoxin oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_01320</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of one alpha, one beta, one delta and one gamma chain.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853328.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5L3"/>
    <dbReference type="GeneID" id="33314869"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01320"/>
    <dbReference type="OrthoDB" id="296931at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03376">
        <property type="entry name" value="TPP_PFOR_porB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051479">
        <property type="entry name" value="PorB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008818">
        <property type="entry name" value="PRK11864.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42897">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42897:SF2">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="3">
        <location>
            <begin position="92"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="311" mass="34590" checksum="4AAF1F876876EEFD" modified="2017-09-27" version="1">MEIPENVKKRLSLPAEEHFYAGHTACQGCGAALGLRYVLKAYGRKTIFTIPACCSTIIAGAWPYNTLDANLFHTAFETTGAVLGGIEAALKARGIKVKGEDGVMVVGWAGDGGTADIGLQALSGFLERGHDALYIMYDNEAYMNTGIQRSSSTPFGAWTTNTPGGKKHFLEKRHKKKVIDIVIAHEIPYAATASVAYPEDFIRKLKKAQKTPGPSFIQLFAPCPTGWRSPTDKSIELARLAVQTAYFPLFEYENGKYKINMPSTKKEPKPIEEFLKLQGRFKYMTKEDIEVLQQWVNREWEKLKKLAEVFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5N0</accession>
    <name>A0A218P5N0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853371.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5N0"/>
    <dbReference type="GeneID" id="33314919"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01570"/>
    <dbReference type="OrthoDB" id="85337at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="193" mass="21464" checksum="492E784DC9EF953C" modified="2017-09-27" version="1">MKVYEPVNLAMPLAKELDEIIMERGGVPDGETVRRILKEFGLEEACLGRGVTVFRNGDVIALLLPRGDVLMVDIIPASGELSDALEVIAYRDKQLNAFIVEILPANDIEYEGNIGLEPAIIDAETLELESSPVLGHFEEDEEGLFLVIDRETYERWKESESTDTCPVCGGELAWKGEGAYCRDCGYRVKVVRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5R8</accession>
    <name>A0A218P5R8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF531 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01645</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853384.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5R8"/>
    <dbReference type="GeneID" id="33314934"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01645"/>
    <dbReference type="OrthoDB" id="53060at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007501">
        <property type="entry name" value="DUF531"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04407">
        <property type="entry name" value="DUF531"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006006">
        <property type="entry name" value="UCP006006"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="20089" checksum="F1BE25E3D222A840" modified="2017-09-27" version="1">MLAIGLYNTYDPKRLHEAHLRAIARAAPIAYAYGFHLALVGFPLEGKPMEVAEEISSHTTIGEGGKYLVELAQNNRFHLLEFPRKGFPPQFGTIVATTRKPSGEKEITPVELAERALGGESFLLLIGLGRHGLPKEIFKTACHHMDITGKRVSLETCTAIGAIPARISTLMEALKWMKGGRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Y7</accession>
    <name>A0A218P5Y7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA polymerase II small subunit</fullName>
            <shortName evidence="15">Pol II</shortName>
            <ecNumber evidence="15">2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Exodeoxyribonuclease small subunit</fullName>
            <ecNumber evidence="15">3.1.11.1</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">polB</name>
        <name evidence="19" type="ORF">A3L08_02015</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 15">Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3' to 5' direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 15">Heterodimer of a large subunit and a small subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the DNA polymerase delta/II small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="15"/>
    <dbReference type="EC" id="3.1.11.1" evidence="15"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853452.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5Y7"/>
    <dbReference type="SMR" id="A0A218P5Y7"/>
    <dbReference type="GeneID" id="33315008"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02015"/>
    <dbReference type="OrthoDB" id="372039at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042575">
        <property type="term" value="C:DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008310">
        <property type="term" value="F:single-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07386">
        <property type="entry name" value="MPP_DNA_pol_II_small_archeal_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.50:FF:000003">
        <property type="entry name" value="DNA polymerase II small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.800">
        <property type="entry name" value="D-family DNA polymerase, DP1 subunit N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00325">
        <property type="entry name" value="DNApol_II_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024826">
        <property type="entry name" value="DNA_pol_delta/II_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011149">
        <property type="entry name" value="Pol2_small_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054750">
        <property type="entry name" value="PolB_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003117">
        <property type="entry name" value="PRK04036.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003118">
        <property type="entry name" value="PRK04036.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416:SF0">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22317">
        <property type="entry name" value="PolB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0235">DNA replication</keyword>
    <keyword evidence="11 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 15" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="9 15" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7 15" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA polymerase II small subunit N-terminal" evidence="18">
        <location>
            <begin position="4"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="17">
        <location>
            <begin position="473"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="73"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="196"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="77"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="218"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF22317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ASJ06189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="739" mass="82339" checksum="A5B9BAAF058AF208" modified="2017-09-27" version="1">MGLVEDLLANRYLITPSAYYLLSDYYKKDFTLAELIKFAKTRGTFVIDSSMAEEFLRGKGILSSGVPLEVSAYEPGDSGEELSEELPSEGEESTYMSHVDVSAPVPPDVGETPVLEPQSESRDKTSISTGTAPESAAVVASSNNLEEETPDFSEGGSFVSTGTIEAEAPTEPSPDETAPNRDMTMEAAGETLPVESEGYAEEASEDVGGLESTAFEQSTPEPESPSEIPVTEATVENGNGYSNGNGNGYADDEEYYENGNGVKPKIIYGDYGIPIAYAAEEVPEEEKNYSVYSDVVIVPKEGFHYRAKEIPDEWEIAFDVKNVKFEVPKVKSAASKEGEIIIKVYSDYFRSRLRKMRRILRENPELGSVIDIGKLSYVRGEDEVTIIGLINNKRETAKGYMFEVEDNTGVIKVFINRNNSESKKFFQIMPDAVVAFRGRYSGRGIFFADKIFLPDVPKFKRTKPPLEEKVYAVLLSDIHVGSNKFCERAFEKFLEWLNGEVNNRKEEELVSRIKYMILAGDVVDGIGIYPGQYNELEIPDIFDQYEALYNLLRNVPDHITMFIGPGNHDAARTALPQPGFYDEYARPLKKLKNAVMISNPAVVRLHGRDFLITHGRGIEDVVSFIPNRSHHRPAEAMLDLLKLRHLAPTFGEKVPIAPDPEDTLVIESVPDLFQAGHVHVMEYRMYNGVFLINSGTWQAQTEFQKMVNIVPTPARVPIIDVETARLRAVVSFDQFCEGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P614</accession>
    <name>A0A218P614_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Metal-dependent carboxypeptidase</fullName>
            <ecNumber evidence="8">3.4.17.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_02235</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Broad specificity carboxypetidase that releases amino acids sequentially from the C-terminus, including neutral, aromatic, polar and basic residues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>Release of a C-terminal amino acid with broad specificity, except for -Pro.</text>
            <dbReference type="EC" id="3.4.17.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the peptidase M32 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.17.19" evidence="8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853492.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P614"/>
    <dbReference type="GeneID" id="33315052"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02235"/>
    <dbReference type="OrthoDB" id="7244at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06460">
        <property type="entry name" value="M32_Taq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1370.30:FF:000003">
        <property type="entry name" value="Thermostable carboxypeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001333">
        <property type="entry name" value="Peptidase_M32_Taq"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34217:SF1">
        <property type="entry name" value="CARBOXYPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34217">
        <property type="entry name" value="METAL-DEPENDENT CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02074">
        <property type="entry name" value="Peptidase_M32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006615">
        <property type="entry name" value="Zn_crbxpep_Taq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00998">
        <property type="entry name" value="CRBOXYPTASET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52034">
        <property type="entry name" value="PEPTIDASE_M32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="10">
        <location>
            <position position="270"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00052755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR006615-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR006615-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="499" mass="59066" checksum="6DE3034B8FA16ABA" modified="2017-09-27" version="1">MESVFQNETVKQILEKYRRIWAIGHAQSVLGWDMEVNMPREGILERSVAQGELSVLSQEFLLKPEFVELVEKAKGIEDLNEYEHGVVRVLDRQIRISRSFPPEFLREMSEVTSQATKAWEEAKRSNDYSKFEPWLDKIIDLAKRAAEYLGYENEPYDALLDLFEEGLTTKEVERMFEKLEKELKPLLEKIMEEGKVPQNHPLEKEKYEREQMERVNLWILEKFGFPLGVRSRLDVSAHPFTTEFGIRDVRITTRYEGYDFRRTILSTVHEFGHALYELQQDERFMFTPIAGGVSLGIHESQSRFWENIIGRSREFAGLIYPVLKENLPFMGNYTPEDVYLYFNMVRPDFIRTEADVVTYNFHILLRFKLERMMLNEGVKAKDLPELWNEEMERLLGIRPKTYAEGILQDIHWAHGTIGYFPTYSIGTLLAAQFYYHIKHDIPDFEEKVAKAEFEPIKAWLREKVHRYGSIYPPKELLKKAIGEELNPDYFIRWVKERYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P615</accession>
    <name>A0A218P615_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_02025</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853454.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P615"/>
    <dbReference type="GeneID" id="33315010"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02025"/>
    <dbReference type="OrthoDB" id="91412at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058836">
        <property type="entry name" value="DUF8183_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058496">
        <property type="entry name" value="DUF8183_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26556">
        <property type="entry name" value="DUF8183"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26555">
        <property type="entry name" value="HTH_78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8183" evidence="2">
        <location>
            <begin position="7"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="DUF8183" evidence="1">
        <location>
            <begin position="138"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="207" mass="23377" checksum="FC2E64C91F34E525" modified="2017-09-27" version="1">MDDVEAKVLEWLKNGNDTAQDIVDLPWALQEVQPNTYLAEHPRMPFSLMVVFSEGFVHLIVPLGLDTFSMSNNEKLKVYHTLLRLNDQVNMMKFTLSGMNDEVYLRVDLDKKTLGKEEFNDALTALLIGLLSSVSALGLEEAFEQEVFDRIVGMVVERMQNGASREELMRFLTIKVGMSVEDATALLEEVFKAKRSMEGSGDDVGYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P638</accession>
    <name>A0A218P638_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Capsular biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_02330</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P638"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02330"/>
    <dbReference type="OrthoDB" id="19148at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd13128">
        <property type="entry name" value="MATE_Wzx_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050833">
        <property type="entry name" value="Poly_Biosynth_Transport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30250:SF27">
        <property type="entry name" value="POLYSACCHARIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30250">
        <property type="entry name" value="PST FAMILY PREDICTED COLANIC ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13440">
        <property type="entry name" value="Polysacc_synt_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="308"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="345"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="400"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="431"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="454"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="509" mass="56524" checksum="3C9EB716E9844DF8" modified="2017-09-27" version="1">MDETGKALQKVARGMGIVLVGTVISMFLTFLSRALIARYFDRYQYGSFTLTMTIMSIAMTVALLGLQSGLPREISLYLRERKEDVPALVSTGLTMAIIASVIMAMVTAYLSPYISPLLNDRYLSETLRLAAPALPFMVLTMLLVAISRGYGRVRENLYFRNLLPPLLFFLILITGILLGFGFTFVFIAYVISQFLSSGIATIGMIRLGLLPRRLHFSPRLAKELFLFSFPLMLTGILDYVMGWTDSLMLGYYFNPDIVGLYNGAAPIARLLPLFLNSMGFLYMPIATAFFTKGDIEGMDKLYRTTARWVFLLTFPVFMFVFVFPESAIGLFFGPKYVEAATALRILSAGFMFHVIMGLNGMSLIAIGEPRANLIGNIFAATANIVLNVLLIPLYGIEGAAVATAVSYIMANIYRTWQLYRKTGVHPFGRNYLKVLMAGLTMIIIMVTTRVNSGVMTAVAATLVAYVLYLPVILLAKTVEKEDVELLEAIEAKFGVNLRPVKKLLLRFVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P642</accession>
    <name>A0A218P642_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02275</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P642"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02275"/>
    <dbReference type="OrthoDB" id="86220at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="203" mass="22682" checksum="C9D2279C4EECB8BC" modified="2017-09-27" version="1">MEDVKELKDVLERIEGKLIAAGKMYGAMNFGAWLSVMLFYYVMLGLFEVNWRFNLIYWPVAFAVAMTFTGRVWKRFKRLGRVTGEVEASRTGAILVALSWTAGIVLGWVIVPRINLGVTSDASLAVGFLTFIALSIFGMWLVFARYGEAEREIVPAFLLPALGIPLARGMESGAMVWAGFLVALGFSVTILWYLYSAFRAIER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P666</accession>
    <name>A0A218P666_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histidine--tRNA ligase</fullName>
            <ecNumber evidence="10">6.1.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Histidyl-tRNA synthetase</fullName>
            <shortName evidence="10">HisRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">hisS</name>
        <name evidence="13" type="ORF">A3L08_02445</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>tRNA(His) + L-histidine + ATP = L-histidyl-tRNA(His) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="10"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853529.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P666"/>
    <dbReference type="GeneID" id="33315094"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02445"/>
    <dbReference type="OrthoDB" id="8659at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00859">
        <property type="entry name" value="HisRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000054">
        <property type="entry name" value="Histidine--tRNA ligase chloroplastic/mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00127">
        <property type="entry name" value="His_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00125">
        <property type="entry name" value="HisZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015807">
        <property type="entry name" value="His-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033656">
        <property type="entry name" value="HisRS_anticodon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004517">
        <property type="entry name" value="HisZ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00442">
        <property type="entry name" value="hisS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00443">
        <property type="entry name" value="hisZ_biosyn_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707:SF1">
        <property type="entry name" value="HISTIDINE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707">
        <property type="entry name" value="HISTIDYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="12">
        <location>
            <begin position="1"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="79"/>
            <end position="81"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="274"/>
            <end position="275"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001549-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="436" mass="49152" checksum="EAB88A908C55B8E5" modified="2017-09-27" version="1">MKRLEKVKGTRDLLPEEMAKRRWVFERIREVFERYNFHEVLTPTFEYTELFKLRSGEEVVEQLYAFDDKGGRNLSLRPDMTSSVARLFVNSFQNAPKPIKWYYMANMFRYEEPQSGRYREFWQAGVELIGSDKVEADAEVIALFVESYLATGLEDFTVNIGDRVLLDEFAKMLGIEDDIGLMRLIDKKDKMSREDFVGALKEFGLSEEGVEKVLALVEIKGLPDEVLPKAEELFTSDEAKAEVARLYELVDLLEAYGVSKWIRIDLGIARGFDYYTSIVFEAIAPNDLGIGSIGGGGRYDNLIEVFGGKPTPATGFAIGIERLIPILEWKGLIPEPKLRPDVYVVPIGKDAEVKRAAVEVVTALREAGVKADYELTGRKLGKALDYAGRLGVPYVVLIGKRDLSEGKVTVRDMDSGEQKAVEREKVVEEILGLLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6B3</accession>
    <name>A0A218P6B3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF112 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_02745</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853587.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6B3"/>
    <dbReference type="GeneID" id="33315155"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02745"/>
    <dbReference type="OrthoDB" id="53365at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002823">
        <property type="entry name" value="DUF112_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42204">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42204:SF1">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01970">
        <property type="entry name" value="TctA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="322"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="353"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="DUF112" evidence="2">
        <location>
            <begin position="5"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="376" mass="40406" checksum="0089A4D05950F316" modified="2017-09-27" version="1">MFRELLIGIAGGTFSGISPGIHVNTLAAFLSGLGVGDNLVLFSMGLTHTFLDVIPSAFLGVPDEGTALGILPAHRLVLRGRAMEVVMIALWASFLAVVLTIPFIPIYMKLAPIYSPRIGRLLIFLLAAFLILTERGTKRLHALAIFILSGILGALTFRLDLNQPFYHLFTGLFGISVILLALRGRGSLPAATDDGDVLMERGRFLRFSFLGTVLGMVASLVPAFTASQAALIGSFLSRDERSFLTVVFSVNTANFLFSFANFVATGRQRNGIVALMNQIPERALTTYLLAALFVSMAVLLYGEPLAALILNLLKRVPYRSMNAGVLLFLVFLSYVFDGLLGILVLAGATITGLLAATMGVKRTCCMGVLMLPIMIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6C3</accession>
    <name>A0A218P6C3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_02740</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853586.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6C3"/>
    <dbReference type="GeneID" id="33315154"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02740"/>
    <dbReference type="OrthoDB" id="86149at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="388" mass="42330" checksum="C19349926A287FEB" modified="2017-09-27" version="1">MVTREKLSIVLIAALLTVLGLLLVTGNERVESKSFVGLCVYSGEGFSVLTDGERTVGVYASLELGKVYRVEGIPFNSTSGLKIRPERVYPSTPTFPLDSITGAYWLSGVSYLLTPAKVRLALPLPADKGELVRVSGLWYGEKFYPVNHTRLGFPKKPSDDMPWAVEGVVLYSGGKTILWNGSEEVVLYLPYGAELKLGQRVRVVGIVRFYSKLSLFVDSPADVVVTGTAEKKPLRKARVGDVAVGNCTAVSAGRSLGLDCTELRLYGFSARVGDSIHFEALWRRSSLICLNCTVTVPREELPNDICSFSPGEFARISGNVSWVRVYKNGFGIANVTSGRCWVLLKLRKSLGVSVRANQTVTAYGFFTTYRDMPAFEVKSGDDLCSGSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6G2</accession>
    <name>A0A218P6G2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF3310 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_02885</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853612.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6G2"/>
    <dbReference type="GeneID" id="33315183"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02885"/>
    <dbReference type="OrthoDB" id="85860at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="124" mass="14539" checksum="7DA95213F17DE74E" modified="2017-09-27" version="1">MVQAYIQEKTKEFSKEEKEKRYKYLGKEVIDVGDPGLDSIPEFYGIANAIWRDWKEGEISTKTALGRLALLKLLTYKTKNKKIADIPEEDLEEVRKFIDYVIQVIKNESRRKGEPEEERQVENA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6K0</accession>
    <name>A0A218P6K0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012572828.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6K0"/>
    <dbReference type="GeneID" id="41609663"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03150"/>
    <dbReference type="OrthoDB" id="35104at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044720">
        <property type="entry name" value="HVO_2753-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011668">
        <property type="entry name" value="HVO_2753-like_ZBP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011481">
        <property type="entry name" value="PRK14890.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40733:SF1">
        <property type="entry name" value="SMALL ZINC FINGER PROTEIN HVO-2753-LIKE ZINC-BINDING POCKET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40733">
        <property type="entry name" value="ZINC-RIBBON RNA-BINDING PROTEIN INVOLVED IN TRANSLATION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07754">
        <property type="entry name" value="HVO_2753_ZBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small zinc finger protein HVO-2753-like zinc-binding pocket" evidence="1">
        <location>
            <begin position="10"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="59" mass="6687" checksum="8389AA85E09436F6" modified="2017-09-27" version="1">MEAKFEIPVCTSCGKEITPREHATHFVCPNCGEEIIWRCESCRVLSVPYKCPKCGWEGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6P4</accession>
    <name>A0A218P6P4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA polymerase sigma factor 70 region 4 type 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853714.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6P4"/>
    <dbReference type="GeneID" id="33315301"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03480"/>
    <dbReference type="OrthoDB" id="94100at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="Sigma3 and sigma4 domains of RNA polymerase sigma factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA polymerase sigma factor 70 region 4 type 2" evidence="1">
        <location>
            <begin position="3"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="130" mass="14881" checksum="08064A3AFC24FC8C" modified="2017-09-27" version="1">MLRLPEGMERVWLMRAKGMREVEIAETLGISRQAVNKALKDARVKLFEAFFGLAEVFSWDVVRVNAEKGFMVARGKCGDENVRVYAFYLPGRGIRAFFNGEFPEYILQHAISIGLIWKKERAELVKVLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6W0</accession>
    <name>A0A218P6W0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cell division protein SepF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853784.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6W0"/>
    <dbReference type="GeneID" id="33315379"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03870"/>
    <dbReference type="OrthoDB" id="56189at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.150">
        <property type="entry name" value="SepF-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007561">
        <property type="entry name" value="Cell_div_SepF/SepF-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038594">
        <property type="entry name" value="SepF-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012426">
        <property type="entry name" value="SepF_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04472">
        <property type="entry name" value="SepF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019313">
        <property type="entry name" value="UCP019313"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="14851" checksum="79283F8391E5262F" modified="2017-09-27" version="1">MGLFDSLKKNDTKTKPARKPPAAVKKEVSASRRDIDVVPLEEDVLAKEIVKPQIRYLKKIVVTSYADLERISEELQSGNIVLVDLTPLEVKPEVLEKVAEQLKGMVNALGGQAAKICKHEIKLILVPSDIRIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6W2</accession>
    <name>A0A218P6W2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosome maturation protein SDO1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_03850</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SDO1/SBDS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853780.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6W2"/>
    <dbReference type="GeneID" id="33315375"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03850"/>
    <dbReference type="OrthoDB" id="84504at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1250.10">
        <property type="entry name" value="Ribosome maturation protein SBDS, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.900">
        <property type="entry name" value="SBDS protein C-terminal domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018023">
        <property type="entry name" value="Ribosome_mat_SBDS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036786">
        <property type="entry name" value="Ribosome_mat_SBDS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002140">
        <property type="entry name" value="Sdo1/SBDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039100">
        <property type="entry name" value="Sdo1/SBDS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046928">
        <property type="entry name" value="SDO1/SBDS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018978">
        <property type="entry name" value="SDO1/SBDS_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037188">
        <property type="entry name" value="Sdo1/SBDS_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019783">
        <property type="entry name" value="SDO1/SBDS_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00291">
        <property type="entry name" value="RNA_SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927">
        <property type="entry name" value="RIBOSOME MATURATION PROTEIN SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927:SF4">
        <property type="entry name" value="RIBOSOME MATURATION PROTEIN SDO1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20268">
        <property type="entry name" value="SBDS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09377">
        <property type="entry name" value="SBDS_domain_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01172">
        <property type="entry name" value="SBDS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89895">
        <property type="entry name" value="FYSH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109728">
        <property type="entry name" value="Hypothetical protein AF0491, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01267">
        <property type="entry name" value="UPF0023"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS N-terminal" evidence="3">
        <location>
            <begin position="8"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS central" evidence="4">
        <location>
            <begin position="103"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS C-terminal" evidence="5">
        <location>
            <begin position="167"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF20268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="236" mass="26693" checksum="62DD8DD78FCCF351" modified="2017-09-27" version="1">MPISVDKAVIARLKTHGETFEILVDPYLAKDFKEGKEVPIEEILATPYIFKDAHKGDKASEHEMEKIFGTSDPYEVAKVILRKGDVQLTAEQRRQMLEDKKRYIATVIHRHAVDPRTGYPHPVDRILKAMDEAGVHVDLFKDAEAQVPGVIKAIRPLLPIKLEMKVIAVKIPSDYVGKAYGEVRKFGNIKREEWASDGSWMFLIEIPGGVEEEFYEKLNALTKGSAITKLIERKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P702</accession>
    <name>A0A218P702_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">MFS transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_04025</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853811.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P702"/>
    <dbReference type="GeneID" id="33315409"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04025"/>
    <dbReference type="OrthoDB" id="85927at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050171">
        <property type="entry name" value="MFS_Transporters"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23517:SF3">
        <property type="entry name" value="INTEGRAL MEMBRANE TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23517">
        <property type="entry name" value="RESISTANCE PROTEIN MDTM, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="209"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="245"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="327"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="359"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="8"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="388" mass="41933" checksum="1D5FAEA41987C71D" modified="2017-09-27" version="1">MERRRLAGIILLIISAFTGTIAFRLATPAIAFYTRDILKASMLSVSIVSMSFVLARAFSSVLGGLMLERGKKLVYVGALAMMGNALSVQLYPLTSTWVQVAGIKLLNGFLNGLSWPMAQFVIAVATPNEIRARVTSVYFFFGSIASLLGNYVYAYTIDLGLGGQMWISSAFFVLTGLIMVASYALLYGRITPKRKKTPDGEKPSLDPRRILIIASLMAVIVAFTSGEITYVYVSEALGMDKATTATLVGWAGFLSAMLSYGISWLADVRSERRMVVLTSIMAAVSPLLAAVKTAPTVFLGIFLALFAFQSFRPISRKVLVSYHRSSLAIGGVNGVQNLSTFLGGMLFGFAYFLGELRGVLTLNLALLAFTPVSLLLLWEAVKLKGGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P748</accession>
    <name>A0A218P748_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14">Mevalonate kinase</fullName>
            <shortName evidence="14">MK</shortName>
            <shortName evidence="14">MVK</shortName>
            <ecNumber evidence="3 14">2.7.1.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">mvk</name>
        <name evidence="17" type="ORF">A3L08_04385</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the phosphorylation of (R)-mevalonate (MVA) to (R)-mevalonate 5-phosphate (MVAP). Functions in the mevalonate (MVA) pathway leading to isopentenyl diphosphate (IPP), a key precursor for the biosynthesis of isoprenoid compounds such as archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(R)-mevalonate + ATP = (R)-5-phosphomevalonate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17065"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13 14">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the GHMP kinase family. Mevalonate kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.36" evidence="3 14"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853871.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P748"/>
    <dbReference type="GeneID" id="33315481"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04385"/>
    <dbReference type="OrthoDB" id="19001at2157"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00098"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004496">
        <property type="term" value="F:mevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000151">
        <property type="entry name" value="Mevalonate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00217">
        <property type="entry name" value="Mevalonate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006205">
        <property type="entry name" value="Mev_gal_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022937">
        <property type="entry name" value="Mevalonate_kinase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00549">
        <property type="entry name" value="mevalon_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003036">
        <property type="entry name" value="PRK03926.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290:SF2">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 14" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="8 14 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11 14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="15">
        <location>
            <begin position="82"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="16">
        <location>
            <begin position="238"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ASJ06614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="334" mass="35342" checksum="F9CECF4FE705B180" modified="2017-09-27" version="1">MRVLASAPAKIILFGEHSVVYGKPAIAAAINLRTYVRAEFNDSGRIKIEAHDIKTPGLIVSFSENEIYFESDYGKAAEVLSYVRQAIELVREEADVNGRGITVSITSQIPVGAGLGSSAAVAVATIGAVSKLLGLELTNEEIGKLGHRVELLVQGASSGIDPTVSAIGGIIHYEKGKFEHLPFMELPIVVGYTGSSGSTKELVAMVRRTYEEMPEIVEPILASMGKVVEKARETLLSELEEGQKLARLGRLMNINHGLLDALGVSTKKLSELVYAARTAGALGAKITGAGGGGCMYALAPERQSEVATAITIAGGTPMITEISREGLRIEEVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P759</accession>
    <name>A0A218P759_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Isopentenyl-diphosphate delta-isomerase</fullName>
            <shortName evidence="11">IPP isomerase</shortName>
            <ecNumber evidence="11">5.3.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Isopentenyl diphosphate:dimethylallyl diphosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Isopentenyl pyrophosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Type 2 isopentenyl diphosphate isomerase</fullName>
            <shortName evidence="11">IDI-2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">fni</name>
        <name evidence="13" type="ORF">A3L08_04415</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in the biosynthesis of isoprenoids. Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its allylic isomer, dimethylallyl diphosphate (DMAPP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>isopentenyl diphosphate = dimethylallyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23284"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="5.3.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10 11">Homooctamer. Dimer of tetramers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the IPP isomerase type 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.2" evidence="11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853875.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P759"/>
    <dbReference type="GeneID" id="33315487"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04415"/>
    <dbReference type="OrthoDB" id="371955at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004452">
        <property type="term" value="F:isopentenyl-diphosphate delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02811">
        <property type="entry name" value="IDI-2_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00354">
        <property type="entry name" value="Idi_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011179">
        <property type="entry name" value="IPdP_isomerase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02151">
        <property type="entry name" value="IPP_isom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43665">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43665:SF1">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003314">
        <property type="entry name" value="IPP_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01240">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 11" id="KW-0288">FMN</keyword>
    <keyword evidence="9 11 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8 11" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0521">NADP</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN-dependent dehydrogenase" evidence="12">
        <location>
            <begin position="21"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="FMN-dependent dehydrogenase" evidence="12">
        <location>
            <begin position="186"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="13"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="71"/>
            <end position="73"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="104"/>
            <end position="106"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="282"/>
            <end position="284"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="303"/>
            <end position="304"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="374" mass="41520" checksum="574E85DCA3C14F8E" modified="2017-09-27" version="1">MQAFDKEELTVIRKFEHIEHCLKRNVQAHVSNGFEDVHFVHMSLPEIDKDEIDMSVEFLGRRFDYPIFIAGMTGGTKGSQLAGRINKTLAKAAQELNIPMGVGSQRAMIRKPETWESYYVRDVAPDVFLVGNLGAPQFAETMPERYGLEEALRAVETIQADALAIHMNPLQESVQPEGDTQYRGVLKALAELKAEFPYPIIAKETGAGVSKEVAIRLESIGIDAIDVGGLGGTSWSAVEYYRVKDEIGKGLALRFWDWGIKTAISVAEVRYSTDLPIIATGGMRDGITIAKALAMGATFAGVALPLLKPAVKGDVEGVIKILRRYIEEIRNAMFLVGARNVEELRKVPLVITGFTREWLEQRIDLREYLRGGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P774</accession>
    <name>A0A218P774_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amino acid permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_04495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P774"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04495"/>
    <dbReference type="OrthoDB" id="101282at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="136" mass="14698" checksum="5EFC7BF4C3D932CC" modified="2017-09-27" version="1">MRVLSFLTSLLVALSFLLPWLRPPSGELTFLHILNEIVTSPNGFEGAFWWLNPSSTGSIFTYVAFFAGLFMILLGVFFGLLGGRLGPGVGLVGMLLFTVIAWYFYGGGFLEILGEGYLLALGSFVAGFLLAGGKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7A5</accession>
    <name>A0A218P7A5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Peptidyl-tRNA hydrolase</fullName>
            <shortName evidence="9">PTH</shortName>
            <ecNumber evidence="3 9">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pth</name>
        <name evidence="10" type="ORF">A3L08_04585</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 9">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = an N-acyl-L-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 9">Belongs to the PTH2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="3 9"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853904.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7A5"/>
    <dbReference type="GeneID" id="33315521"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04585"/>
    <dbReference type="OrthoDB" id="6075at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02430">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1490.10:FF:000001">
        <property type="entry name" value="Peptidyl-tRNA hydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1490.10">
        <property type="entry name" value="Bit1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00628">
        <property type="entry name" value="Pept_tRNA_hydro_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023476">
        <property type="entry name" value="Pep_tRNA_hydro_II_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034759">
        <property type="entry name" value="Pept_tRNA_hydro_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002833">
        <property type="entry name" value="PTH2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00283">
        <property type="entry name" value="arch_pth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003314">
        <property type="entry name" value="PRK04322.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649:SF11">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01981">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102462">
        <property type="entry name" value="Peptidyl-tRNA hydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00050038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="118" mass="12956" checksum="F4E3E2CB7C8BA3B5" modified="2017-09-27" version="1">MFKYKQVIVSRKDLKLSKGKFAVQVAHGAVTAALKAQKEKPEWFKAWFHEGQKKVVVKAENLEELFKLKAEAEKLGIPTALIKDAGLTEIPPGTITVLAVGPAPEELVDKVTGHLKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7A6</accession>
    <name>A0A218P7A6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">acetate--CoA ligase (ADP-forming)</fullName>
            <ecNumber evidence="3">6.2.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_04670</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP + CoA = acetyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15081"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the acetate CoA ligase beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.13" evidence="3"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853921.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7A6"/>
    <dbReference type="GeneID" id="33315538"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04670"/>
    <dbReference type="OrthoDB" id="18103at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043758">
        <property type="term" value="F:acetate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000020">
        <property type="entry name" value="Protein lysine acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051538">
        <property type="entry name" value="Acyl-CoA_Synth/Transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43334:SF1">
        <property type="entry name" value="3-HYDROXYPROPIONATE--COA LIGASE [ADP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43334">
        <property type="entry name" value="ACETATE--COA LIGASE [ADP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13549">
        <property type="entry name" value="ATP-grasp_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="9">
        <location>
            <begin position="27"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="232" mass="25753" checksum="E473E34205030BEA" modified="2017-09-27" version="1">MDRIEKAKAIIEKAKAENRPLVEPEAKEILKLYGVPVPDFKVATNEEEAVKFAREIGYPVVMKIVSPQIIHKSDAGGVKVNIKNDEEAKQAFRTIMENARNYKPDADLWGVIIYRMLPLGKEVIVGMIRDPQFGPAIMFGLGGIFVEILKDVSFRVAPITKDEALDMIKEIKAYPILAGARGEKPVDIEALADIIVKVGELALELPEIKELDINPIFAYEDSAVAVDARMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7B6</accession>
    <name>A0A218P7B6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_04645</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transcriptional regulator TrmB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853916.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7B6"/>
    <dbReference type="GeneID" id="33315533"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04645"/>
    <dbReference type="OrthoDB" id="30795at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053499">
        <property type="entry name" value="HTH-type_TrmB_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051797">
        <property type="entry name" value="TrmB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021586">
        <property type="entry name" value="Tscrpt_reg_TrmB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041141">
        <property type="entry name" value="tran_reg_TrmBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293:SF1">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11495">
        <property type="entry name" value="Regulator_TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="5">
        <location>
            <begin position="10"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription regulator TrmB C-terminal" evidence="6">
        <location>
            <begin position="126"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="264" mass="30729" checksum="9653823B47076E67" modified="2017-09-27" version="1">MVKDKMVELLQEHFELNLYEARAYVALVGFGVLTPAELASVSEVPAPRTYDVLRSLEKKGFAISQPGKVNKYRPVHPENILEKFIEEWQERVKEELEAKKKAKEELLQLMRPLIETEIPKYGVEKVWVVRGIRNATLKTKEMFEEVKEQILLADDGYIAINLESDLIKAIDNGAKAKIIVTPNVLQRLGTSKIMDYYKKGKLELKVLDKFELPMLICDDEVFFALEDMAARYFNYETQIWIKDFRVKALFEAKFNEYWEKAKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7C4</accession>
    <name>A0A218P7C4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_04700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853927.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7C4"/>
    <dbReference type="GeneID" id="33315544"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04700"/>
    <dbReference type="OrthoDB" id="10444at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051827">
        <property type="entry name" value="Cas4_exonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038726">
        <property type="entry name" value="PDDEXK_AddAB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36531">
        <property type="entry name" value="CRISPR-ASSOCIATED EXONUCLEASE CAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36531:SF2">
        <property type="entry name" value="CRISPR-ASSOCIATED EXONUCLEASE CAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12705">
        <property type="entry name" value="PDDEXK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PD-(D/E)XK endonuclease-like" evidence="1">
        <location>
            <begin position="50"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="224" mass="26162" checksum="F2D610F8B584DF1A" modified="2017-09-27" version="1">MENPDRLETFNREIRNRIAGRRSPRKIWVTSLSFCLRKAALSTYLETFKYERTGEMLLGTLLHRWVQENVDLEEIEFEVPLEYEITDGWVLVGRADAILGDKVLEFKLKGFNPENGPGSLDEMEEPSKIWKEQLNAYLNMAGLEEGYIYVFDRDGLDFRHFKVEREGEAFSKMLGRARVVINGVEALEQGKFPAWIRPRYSRECESCIFRPICGAIDKPWEKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7D0</accession>
    <name>A0A218P7D0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRISPR system Cms protein Csm4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_04695</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853926.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7D0"/>
    <dbReference type="GeneID" id="33315543"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04695"/>
    <dbReference type="OrthoDB" id="102299at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="34568" checksum="0013C6F6CB344469" modified="2017-09-27" version="1">MYLVTLRFRAYPLSFSLSSPNELSQELMPLDGPLAFAEYYRTKLSHDPDWRIKYLEDVLTARELEWVSNIRSSYLLPCTVNEERLTITTPMGFKVVFNVNGEARYEFSERQHPKKWESSQIFLRLGRRDDIIKVEILRNSLGVSLTKRTKGLPSSQSGPYKAVDYSLRLYTPNLMWASIVDGISQRKLRELLYILRKFGVGKKRNMGWGDLLEYHIYELKSRNITSSYILHAQGESRFLETWRPISPEKIAGMITKPPKGVEYNRLSLLDSKIGYGAERPPYWRRNLVVKSALFLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7D3</accession>
    <name>A0A218P7D3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1850 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_04785</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7D3"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04785"/>
    <dbReference type="OrthoDB" id="103636at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015001">
        <property type="entry name" value="DUF1850"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08905">
        <property type="entry name" value="DUF1850"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="17237" checksum="A9163E0C18833EA0" modified="2017-09-27" version="1">MRGPPPLFFVFLLLAPFLVPTHALVISDGHSSRVYPLVGDVMVEIDYIHSVERSEIREVLEANSSGLYAKEMWWKDFGAGLPEDIQYMEDGFYVKKTNIPLGRSLDFWFIPLNKAEIKVNGETVFAPREETLMRFRVKRCILVETLLGRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7E0</accession>
    <name>A0A218P7E0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glycogen phosphorylase</fullName>
            <ecNumber evidence="4">2.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">A3L08_04880</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + phosphate = [(1-&gt;4)-alpha-D-glucosyl](n-1) + alpha-D-glucose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:41732"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9586"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycogen phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.1" evidence="4"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853955.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7E0"/>
    <dbReference type="GeneID" id="33315580"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04880"/>
    <dbReference type="OrthoDB" id="17863at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008184">
        <property type="term" value="F:glycogen phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011834">
        <property type="entry name" value="Agluc_phsphrylas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000811">
        <property type="entry name" value="Glyco_trans_35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052182">
        <property type="entry name" value="Glycogen/Maltodextrin_Phosph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053611">
        <property type="entry name" value="Glycogen_Phosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024517">
        <property type="entry name" value="Glycogen_phosphorylase_DUF3417"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035090">
        <property type="entry name" value="Pyridoxal_P_attach_site"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041129">
        <property type="entry name" value="maldex_phorlase_Thcocales"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02094">
        <property type="entry name" value="more_P_ylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42655">
        <property type="entry name" value="GLYCOGEN PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42655:SF1">
        <property type="entry name" value="GLYCOGEN PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11897">
        <property type="entry name" value="DUF3417"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00343">
        <property type="entry name" value="Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000460">
        <property type="entry name" value="Pprylas_GlgP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00102">
        <property type="entry name" value="PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DUF3417" evidence="11">
        <location>
            <begin position="16"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="10">
        <location>
            <position position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000460-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF11897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="830" mass="96894" checksum="5C2EA7C581DFFE78" modified="2017-09-27" version="1">MADVSHAVEDSIRAKLPYPLGDLADLAYNYWWSWNRRATKLWEYIDPEHWREYKNPVKLLLDTPEERFRELLKDDDFMNLYELVMDQFTAYMNPRSTWFSTNYPKWDKPIVYLCMEYGISKSLPIYSGGLGILAGDHVKTASDLGLPFIAIGLLYKHGYFRQEIDENGRQREIFPEYRPEEMPIKPVLGKDGKPLLIEVPIEDRIVYARAFEVNVGRVKLYLLDTDVPENSPEDRTVCDYLYNAEMDKRIKQEILLGIGGMRLLKALGIEPGVIHLNEGHPAFANLQRIAWYTEKGLTFTEALSIVRGTTIFTTHTPVPAGHDRFPIEEVRKRLSKFLEGREELLELGREGDQINMTLLAIRTSSYVNGVSQLHAEVSKRMWKDLWPGVPLNEIPIEGITNGVHTMTWVHNSMRKLFDRYIGKVWREHTNLEGIWYAVEMIPDEELWEAHLEAKRQFIELLREKIMERNKRLGTDDPIPEIDENALIIGFARRFATYKRATLILSDLERLKKILNNPERPVYIVFGGKAHPRDEAGKEFLRRVYEVSQMPEFRGKIFVLENYDMGSARLMVAGVDVWLNNPRRPLEASGTSGMKAGLNGVLNASIYDGWWVEGYNGKNGWVIGEETTEPETEEDDVKDAESLYTLLEEEIIPTYYDNRERWIYMMKESIKSIAPRFSTHRMVKEYMDKFYSKAMSNYIWLTRENYRGAKEIAAWKDRVTSSWDRVEIKEAKVEGSRLDVRVYLDGLKPEDVEVELYYGVRAQGYEIEKPHIVELRHPQEVGEGEWLYVYEGNALRHLGDPCWHYAVRIHPHHEKLPHRFLLGFVKWRDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7E9</accession>
    <name>A0A218P7E9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycerophosphodiester phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_04930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853962.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7E9"/>
    <dbReference type="GeneID" id="33315590"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04930"/>
    <dbReference type="OrthoDB" id="19020at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08568">
        <property type="entry name" value="GDPD_TmGDE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46211:SF14">
        <property type="entry name" value="GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46211">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GP-PDE" evidence="1">
        <location>
            <begin position="12"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="255" mass="29104" checksum="05B59412D91CA43C" modified="2017-09-27" version="1">MRAGTEVNNESTLILGHRGFKGPLENTLPAFRRALLYADGVEFDVRVTGDGKLVAHHDEGFWSDGLFYRINGLSLRELRSIHPLGKLVPRVEDIFRTFRNSFFDADVKEPEAVEPLLKLVERFSVAGRVVFSSENPGIVKALLRECPDCRVGFSIVGYSSLVWIPRLKGLYSLHVPIDAVSYVGYRTFVTLLEALRKRGLRIYLWNYRMNELEWAPRLWRFADALILDNPVWVKKGFYGYGALFGGDTYDELGIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7F0</accession>
    <name>A0A218P7F0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_04940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853964.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7F0"/>
    <dbReference type="GeneID" id="33315592"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04940"/>
    <dbReference type="OrthoDB" id="98797at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="409"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="433" mass="48749" checksum="AAC3F9AB754B5086" modified="2017-09-27" version="1">MDSRWILTLIATVLLVSLPIPGEIYSPPNVPYLNGNYDVYQEVPSRVATLLCGAETREYYEYLVNWTLRFPHYHVYRGAGGSLRTAIVVGNWSSFEKSLPYGCEIISLKELSKEPTISHDPALYNASLLLENLTKGTPDYEKLKPFFSFASYSPKNSELPKTNNTHVEVIIIGTRGTEFVWKPFVFVWAIVMVLSAVGLFTSWKRRKKVIFVSFVIILLLGAFFVGKVLYLEHEKAEWNSNVASVLSLKGTGGECWPVVGAINVPSDSGADSIPRALQLINESYAKVLRVSFEDYIVKLEVGVNPESYEELAKEAEELGLQVDIFPKSDVLRIIDSRKKEYEMKKEMIATLDKYLPKLPKAERESVERFIELQKQFLEETEKQLNASKNCYEVGIAIPTEYGVIQYSSLSGFLAKLALLVVGIALVVSWKNDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7F5</accession>
    <name>A0A218P7F5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protease PrsW</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_04975</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protease PrsW family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853970.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7F5"/>
    <dbReference type="GeneID" id="33315599"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04975"/>
    <dbReference type="OrthoDB" id="248468at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023596">
        <property type="entry name" value="Peptidase_PrsW_arch/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026898">
        <property type="entry name" value="PrsW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36844">
        <property type="entry name" value="PROTEASE PRSW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36844:SF1">
        <property type="entry name" value="PROTEASE PRSW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13367">
        <property type="entry name" value="PrsW-protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016933">
        <property type="entry name" value="PrsW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="66"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="170"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="233" mass="25687" checksum="F242BD3C13B6D7C9" modified="2017-09-27" version="1">MNALDALIFFAYAPALAILWYLYHADRYEPEPRRYVIGTFILGGTLSVGIAYILEGVLTVGGMIKPLLPATAFYVALVAGLVEEPSKALAIRWPYKRGQMDGIMDGLVYGAAAGLGFAATENFMYGLGWGLGVTITRAFLTPFAHATWSAIIGVGYGLKAEGKINSVGHYFLLAIFLHFIWDYYAFLSASVPAYNIVLIVLIGVNLAILRYFMIAGLAEDRSRLLYYWFRRGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7H8</accession>
    <name>A0A218P7H8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7H8"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05105"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="47"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="373" mass="43210" checksum="196AC8486E26A115" modified="2017-09-27" version="1">MRKWVAMLLLGIPLLSSVQTVKAGTTQTTNQAHTCQSCSCIDVNTLLDNLTANITNLTHTIDQKKLRLEKLYEKWNTTKNMSILFEIVMQKDEIRLLTNELKYLKRQKLSLQLVQNYTVQTPYGRKILYYKPPPESTIVQEHVKKVHPVRSDVDLAWFIAYYRQAAELTFTEVVQTDMRMRELLKKVEAGNYTEETINEILTLLDKREKLWREIYKFAEEKEKLQTLKTLKETGRKQTLIKAATGVEPLAIKFGGIAKGCFCSYLHCYNSYGTAPPLPGVIHPLDASEAPYMYYREFYPVVLWVGMYTSSEPDVNHFWGDYCTYEFPSTGTDFEKYWNRAIELGLQTFGSIQIKLFYEGAHTIQKCRAIKKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7M2</accession>
    <name>A0A218P7M2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nonsense-mediated mRNA decay NMD3 like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854042.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7M2"/>
    <dbReference type="GeneID" id="33315682"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05390"/>
    <dbReference type="OrthoDB" id="15051at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039768">
        <property type="entry name" value="Nmd3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007064">
        <property type="entry name" value="Nmd3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746:SF2">
        <property type="entry name" value="60S RIBOSOMAL EXPORT PROTEIN NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04981">
        <property type="entry name" value="NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nmd3 N-terminal" evidence="1">
        <location>
            <begin position="6"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="388" mass="44993" checksum="8A87894BF23206EA" modified="2017-09-27" version="1">MGERFCYRCGISESEGGPLIEGLCQVCYRKENPVLLIEGEVNTELCQNCGSYKKRGVWVDPQSYELDELIFEVADNALMEALEDSLSDKTREYEVVSMEELDEVDDLPLGRAVIAFQPVDWHIEYFPAIITYEVRVKARTHELQRELHDERTHVTVYVRQTVCPRCSRFLGGYFEAILQVRADGRPLTHEERKVIGKLVEEKVDEIMQKDRMGFIQDTIEKEEGLDFYMGSTSSARKLAQAIKERFGGTISEAYELVGVDRQTSRETYRTSVSIRIPKFRRGDIVADKRGNVYEVEKVDGRGLSLRNLATEESEHLTWKTVKREELDTVEHEKSEAMVTSITPNEVQLMDMRTYETYELERPKMNLKEGEIYNMVEVKGRRYFLSKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Q2</accession>
    <name>A0A218P7Q2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">OBG-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_05475</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854058.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Q2"/>
    <dbReference type="GeneID" id="33315699"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05475"/>
    <dbReference type="OrthoDB" id="147673at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01897">
        <property type="entry name" value="NOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041623">
        <property type="entry name" value="NOG1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010674">
        <property type="entry name" value="NOG1_Rossman_fold_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45759">
        <property type="entry name" value="NUCLEOLAR GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06858">
        <property type="entry name" value="NOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17835">
        <property type="entry name" value="NOG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="3">
        <location>
            <begin position="168"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="358" mass="41397" checksum="1531D79F38379D33" modified="2017-09-27" version="1">MKNPFEKMPTVLTADELIDKAFRRAEKAASALTPKGGPRAKAREREELRVRTVSNVVRDNLRKLLDRTPGVSELPEFYRELVDTLVDRDQFHRSLGRVNWAIKTIRELEKRHVEKIRYLRDPAEIAKVRRSFYGRVADVIKSIADDLEYLNQARNVLKDLPVVDLELPTVVIAGHPNVGKSTLLRALTNAKPEVASYPFTTKGINVGQFEEHHLKYQVIDTPGLLDRPLSERNEVERQAILALKHLGKVIVYIFDPSEYCGYPIEEQIHLFEEIHREFGEFPFIVVLNKVDIADEEKIKAIEEFVMAKGLEPLRISALNGEGLDELKRRVVELVKPMVEEQARKIMERELRKYREEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7U7</accession>
    <name>A0A218P7U7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bifunctional protein GlmU</fullName>
            <ecNumber evidence="5">2.3.1.157</ecNumber>
            <ecNumber evidence="6">2.7.7.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">A3L08_05490</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N-acetyl-alpha-D-glucosamine 1-phosphate + UTP + H(+) = UDP-N-acetyl-alpha-D-glucosamine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="2.7.7.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>alpha-D-glucosamine 1-phosphate + acetyl-CoA = N-acetyl-alpha-D-glucosamine 1-phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13725"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="EC" id="2.3.1.157"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II): step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.157" evidence="5"/>
    <dbReference type="EC" id="2.7.7.23" evidence="6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854061.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7U7"/>
    <dbReference type="GeneID" id="33315702"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05490"/>
    <dbReference type="OrthoDB" id="15372at2157"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00532"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019134">
        <property type="term" value="F:glucosamine-1-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003977">
        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05636">
        <property type="entry name" value="LbH_G1P_TT_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023915">
        <property type="entry name" value="Bifunctiontional_GlmU_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050065">
        <property type="entry name" value="GlmU-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03992">
        <property type="entry name" value="Arch_glmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43584:SF8">
        <property type="entry name" value="N-ACETYLMURAMATE ALPHA-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43584">
        <property type="entry name" value="NUCLEOTIDYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="14">
        <location>
            <begin position="2"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="15">
        <location>
            <begin position="268"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ASJ06810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="419" mass="46080" checksum="B6D96265C28A5429" modified="2017-09-27" version="1">MKGVILAAGKGERLRPLTDDRPKVILKVANRPIIDYVLENLDPFVDEFIVVVRYEKEKLIKALGDEFNGKPITYVEQLPGKGTAKAIESAKGSVGDEEFIAVNGDIYFEIDGVKELVSTFRKEKADAALLVKEFDDLSQFGKIEVEGNRVKAVLEKPGRVSGYANLGVYIFRPEVFEFIEKTPLSERGEYEITDTLNLMIEEGKTVVYAPYSGYWNDIGRPWNLLELNEYLLKTKLRHSIRGIVEEGATIVPPVEIGEGTVVRSGAYIIGPVKIGKNSRIGPNCFIRPYTSIGDNCHVGNAVEIKNSIIMDNSNAPHLNYVGDSIIGENSNLGAGTITANLRHDRGNVRVEIKGKLEDSGRHKLGAIIGHGVKIGINVSIYPGRKIGSGSFIGPGAIVDRNVPPKSLLIVKQEKEVRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7U8</accession>
    <name>A0A218P7U8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA pseudouridine synthase A</fullName>
            <ecNumber evidence="4">5.4.99.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">tRNA pseudouridine(38-40) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA pseudouridylate synthase I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA-uridine isomerase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">truA</name>
        <name evidence="9" type="ORF">A3L08_05665</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 7">
            <text>uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:22376"/>
            <dbReference type="Rhea" id="RHEA-COMP:10085"/>
            <dbReference type="Rhea" id="RHEA-COMP:10087"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 7">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.12" evidence="4"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854093.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7U8"/>
    <dbReference type="GeneID" id="33315737"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05665"/>
    <dbReference type="OrthoDB" id="25720at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160147">
        <property type="term" value="F:tRNA pseudouridine(38-40) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.580:FF:000001">
        <property type="entry name" value="tRNA pseudouridine synthase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00171">
        <property type="entry name" value="TruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00071">
        <property type="entry name" value="hisT_truA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF0">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001430">
        <property type="entry name" value="tRNA_psdUrid_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase I TruA alpha/beta" evidence="8">
        <location>
            <begin position="8"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Pseudouridine synthase I TruA alpha/beta" evidence="8">
        <location>
            <begin position="129"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4 5">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 6">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001430-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001430-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="267" mass="30835" checksum="4C57403BD04FA718" modified="2017-09-27" version="1">MKFALRIAYDGTAFYGFQRQPDVRTVEGEILRALTRLRVIEDAESAGFKGASRTDRGVSAIFNVVAFDAAGRPDLVRAEVLNHHLRDVWVLGVAEVPDDFHPRFWAKSKTYRYYLVNEGFDRWKMEECARLFIGEHDFSAFARLEPGKEPVREITRVEIEERQGYYVIEIKGKSFLWEMVRRMVNAIRFCGLGLMEPAEIERMLNGDYTKKVPPALPEGLVLWHIDYPGIKFEVDERGLKKAKRDIFERYSRALTRAALLGDALLQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Y3</accession>
    <name>A0A218P7Y3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bacterial Pleckstrin homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05935</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Y3"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05935"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="15865" checksum="B38C8CA334BBFE6E" modified="2017-09-27" version="1">MGGWFPLVVVLTPIVVIALVVAVKIKGYMDAYVPWSIVVAKNGTVRLIIKTKAGEREILVRDFDVKESSEVLEVRINGLGFGRYQLGEYKGPFGYVKSYAVSRKGLLVTDVRGKRYYLAFEKVDDVLKALRGGPGKTEIKVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Z1</accession>
    <name>A0A218P7Z1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_05995</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854156.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Z1"/>
    <dbReference type="GeneID" id="33315803"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05995"/>
    <dbReference type="OrthoDB" id="350705at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1640.10">
        <property type="entry name" value="inorganic pyrophosphatase (n-terminal core)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051319">
        <property type="entry name" value="Oligoribo/pAp-PDE_c-di-AMP_PDE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47618">
        <property type="entry name" value="BIFUNCTIONAL OLIGORIBONUCLEASE AND PAP PHOSPHATASE NRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47618:SF1">
        <property type="entry name" value="BIFUNCTIONAL OLIGORIBONUCLEASE AND PAP PHOSPHATASE NRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDH" evidence="1">
        <location>
            <begin position="20"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="2">
        <location>
            <begin position="248"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="327" mass="35987" checksum="4E2F7D44CAD6B3B1" modified="2017-09-27" version="1">MKGKIKLRRFLESSRGRSFLLLCHHNADPDSLGSAIAFAIYLKSLGVEEVKIGVAQSVSSYAKRLLTFSPVPVERDPVVKEDVVMIFDTSSLEQLEPIEIPRGKTVIVIDHHVEKEKPIKAQIAVVDSSRTSTAEIVWELFKYLGFYNETAVKALLAGIVTDTANFRFANAKTFKAVSEMLERFPLQMGGIFQLVAPVSDENIDQAKRMAMLKACQRLEIKKFRRYIIAVSKVSAYESLACKTFLNLGADVAIVGSEKKGVRISARAKESLVKKGLHLGRIMEKVGPIIEGSGGGHAGAAGANGKKNLDEAIKVILKEIEKFLREVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Z8</accession>
    <name>A0A218P7Z8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854166.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Z8"/>
    <dbReference type="GeneID" id="33315815"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06060"/>
    <dbReference type="OrthoDB" id="84876at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="86" mass="9973" checksum="579CAA80E17B9A65" modified="2017-09-27" version="1">MLFKKRGVFTEEDARKVVEWMSTHPEERELIVMAESVTAEAKRRLWDYARAVQLMADMTGEDRSVKVEVLEGFVSDRVKGLNVEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P825</accession>
    <name>A0A218P825_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cation:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_06230</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854196.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P825"/>
    <dbReference type="GeneID" id="33315849"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06230"/>
    <dbReference type="OrthoDB" id="85180at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002758">
        <property type="entry name" value="Cation_antiport_E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34584">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER SUBUNIT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34584:SF1">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER SUBUNIT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01899">
        <property type="entry name" value="MNHE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019239">
        <property type="entry name" value="MrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="176" mass="19762" checksum="110FCE9054837B43" modified="2017-09-27" version="1">MEEASKISRYLYTVIVLFLIWLLLTASLDPQELGFGLILSLIVAAFTYEIFSTGGLANLHPKRVAYAIAYVPYFLWAMIMANLDVAYRVLHPKRPIRPGIVRCKTVLNSDVGKLALANSITLTPGTITLDVDGDNYFIHWIWVPDEAMTEKEEEHVKAASEGITVPFEKFLKVIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8A5</accession>
    <name>A0A218P8A5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Membrane bound complex 1 subunit E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_06555</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8A5"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06555"/>
    <dbReference type="OrthoDB" id="98852at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025383">
        <property type="entry name" value="MrpA_C/MbhD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13244">
        <property type="entry name" value="MbhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="MrpA C-terminal/MbhD" evidence="7">
        <location>
            <begin position="12"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="80" mass="8506" checksum="F73569DE14BAA78F" modified="2017-09-27" version="1">MIELTALALLGCLGLAYLVVTERDLLKAVLYTGIFGGLVIIATYTLMAPDIILAYVAVSVALSTGLMVFLVSKTTREEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8C4</accession>
    <name>A0A218P8C4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aminotransferase</fullName>
            <ecNumber evidence="7">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_06685</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854279.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8C4"/>
    <dbReference type="GeneID" id="33315940"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06685"/>
    <dbReference type="OrthoDB" id="372018at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000033">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050596">
        <property type="entry name" value="AspAT/PAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383:SF3">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="8">
        <location>
            <begin position="27"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="389" mass="43818" checksum="1CD0F3A899D291A2" modified="2017-09-27" version="1">MALSDRLELVNPSEIRKLFDLAAGMEDVISLGIGEPDFDTPEHIKEYAKEALDKGYTHYGPNAGLPMLREAVARKLKKQNGIEADPKTEVMILTGANQAFIMGFATFLKDGEEVLIPSPMFVSYAPAVVLAGGKPVEVPTYEENEFRLSVDDLEKHVTEKTRALIINTPNNPTGSVLTKKDIEEIADFAVEHDLMVISDEVYEHFVYDGVKNYSIASLDGMFERTITVNGFSKTFAMTGWRLGFVAAPDWIIEKMTRFQMYNSTCPVTFVQYAAAKALDDPRSWEAVEEMRKEYDRRRNLVWKRLNEMGLPTVKPKGAFYIFPRIKDTGLTDKEFSELMLKEAKVAVVPGSAFGKAGEGYIRISYATAYEKLEEAMDRMEKVLKEKKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8E6</accession>
    <name>A0A218P8E6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_06710</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854284.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8E6"/>
    <dbReference type="GeneID" id="33315945"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06710"/>
    <dbReference type="OrthoDB" id="57092at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43483">
        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN HI_0806-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43483:SF3">
        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN HI_0806-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="254" mass="26766" checksum="8623F672C87FF901" modified="2017-09-27" version="1">MLKYLGYFGVGIFIGILAAMFGLGGGFLVVPTLNFLGVEIHHAVGTSSAAVVFTSLSSAIAYHRQRRIHYKAGLLLASTAVVGAYIGAWATSYISASQLKVIFGIVLFLVAIRIYRKKSAEPHEVRLEDVELDYRLIPVGGFIAGIASGLLGIGGGAINVPFLTAMGLPIHYAVATSSFAIVFTATSGALKHYMMGNVEVEWLLLLVPGLIIGAQLGARIAKRTKASSLGKAFAVVLAFLAIRMVLKGLGFAVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8F2</accession>
    <name>A0A218P8F2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">S-layer protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06850</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854309.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8F2"/>
    <dbReference type="GeneID" id="33315973"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06850"/>
    <dbReference type="OrthoDB" id="148350at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="613" mass="69916" checksum="DE725971C991195D" modified="2017-09-27" version="1">MKRLFALIITLLFIVLPPAEVAGVAQHSADSEVVAEIDPNAELLGVVYYLAFGRDDPFVIDRGSYLDDVEAWFGPYRDHPAVKILRDYLGGRTSIPDRDYQLMLIEYYLLLCSGPPEIEPLVPPNFPWFEDEFLPALRDFARKTGFMKFYSSHEDHYAEDLTIYTGALHMLPPDGFMEDYSGFSGVQYRFEHPFLVAVHGHSFNPVINGTQIWGAGGMLPLVRRTPQRTLWSYRTARDTMFGLPLNRDIINSTYLNELLYLGFIYHELGHDVTLPALYASYAAVQNLTYLQEAIEEDMPYLARYDMHFWSEDGMLYEGFADAWEDFALAHVDGNYTRLAMEMQKAWGEFWIERLFYGVQDCALSVKAGEVDDFSACVPRLLGSLQRFASPENVSTVYEMEVPVTPLRAFDRGAQAGKVIVVYGTRNPDPSGTDYDRETADIIAENLRKFYSSWPANVDVEVKADVSVTDEDLSGNLVLVGGPVANSIAADMQKDFPFGFVREDGKWVIWRNESWWYGWKPDYFIILADDSPYRRAFNSTIIRGHFGIAEDASLIMAVRNPNNPENYIVWIAGTDRNLTRLFTNPTYYLSSYEVYSKEYKKIEVGFYVQPLASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8F3</accession>
    <name>A0A218P8F3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="12">5-formaminoimidazole-4-carboxamide-1-(Beta)-D-ribofuranosyl 5'-monophosphate synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">A3L08_06910</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854321.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8F3"/>
    <dbReference type="GeneID" id="33315985"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06910"/>
    <dbReference type="OrthoDB" id="98133at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006188">
        <property type="term" value="P:IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023656">
        <property type="entry name" value="IMP_biosynth_PurP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009720">
        <property type="entry name" value="IMP_biosynth_PurP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010672">
        <property type="entry name" value="IMP_biosynth_PurP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009782">
        <property type="entry name" value="PRK13278.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38147:SF2">
        <property type="entry name" value="5-FORMAMINOIMIDAZOLE-4-CARBOXAMIDE-1-(BETA)-D-RIBOFURANOSYL 5'-MONOPHOSPHATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38147">
        <property type="entry name" value="5-FORMAMINOIMIDAZOLE-4-CARBOXAMIDE-1-(BETA)-D-RIBOFURANOSYL 5'-MONOPHOSPHATE SYNTHETASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06849">
        <property type="entry name" value="DUF1246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06973">
        <property type="entry name" value="DUF1297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMP biosynthesis enzyme PurP N-terminal" evidence="10">
        <location>
            <begin position="3"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="IMP biosynthesis enzyme PurP C-terminal" evidence="11">
        <location>
            <begin position="157"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ07071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="321" mass="36288" checksum="F06EB99E57FC1ECD" modified="2017-09-27" version="1">MIISTIASHSSLQILLGAKDEGLKTRLYVKPGRKAFYASTRLADELVVTENMDAILNDEGIVVPHGSFVAYLGLEAIEKAKVRFFGNRRFLKWETTFELQDRALDKAGIPRVEVVEPEDIRSDELYFVRLEGPRGGSGHFIVRGDEIEERLKRVEGNYRVERFIPGVYLYVHFFYSPILDRLELLGVDERVVIADGNARWPVKPLPYTIAGNVGVALRESLLPRLYDYGLAFVEAMRKLEPPGIIGPFALHFAYDGEFKAIGFASRIDGGSNALHWYGRLYWDEPMSAGRRIAREIKLALEEERLEEVVTWPTQAGRSGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8F5</accession>
    <name>A0A218P8F5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2-oxoglutarate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_06775</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854294.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8F5"/>
    <dbReference type="GeneID" id="33315958"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06775"/>
    <dbReference type="OrthoDB" id="30755at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03375">
        <property type="entry name" value="TPP_OGFOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051457">
        <property type="entry name" value="2-oxoacid:Fd_oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48084">
        <property type="entry name" value="2-OXOGLUTARATE OXIDOREDUCTASE SUBUNIT KORB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48084:SF1">
        <property type="entry name" value="2-OXOGLUTARATE SYNTHASE SUBUNIT KORB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="2">
        <location>
            <begin position="57"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="284" mass="30951" checksum="83DE9757778A8B34" modified="2017-09-27" version="1">MAKEIYSKYPLIKYLRKEALPTALCPGCGGGTVLNAFANAVDGLKIDPKDLVVVSGIGCSAWIASPYFLADTLHTTHGRAIAFATGVKVGLPDKKVVVISGDGDLASIGGNHLLHAARRNIDITVILVNNMIYGMTGGQVAPTTPFGAKTTTSPYRNIEHPLNISETVAAAGASYVARWTTAHVYQLIESIKKALQVKGFSLVEVVSQCPVQFGRRNRMKEPAEMLRWFLKNSVPVSKAKKMKPEELEGKFVIGEFVNRERPEFVTELNKLIDEVQEHFGLKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8F7</accession>
    <name>A0A218P8F7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Dipeptide/oligopeptide/nickel ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_06940</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854326.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8F7"/>
    <dbReference type="GeneID" id="33315991"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06940"/>
    <dbReference type="OrthoDB" id="18209at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43230">
        <property type="entry name" value="ABC-TYPE DIPEPTIDE/OLIGOPEPTIDE TRANSPORT SYSTEM, ATPASE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43230:SF3">
        <property type="entry name" value="ABC-TYPE DIPEPTIDE_OLIGOPEPTIDE TRANSPORT SYSTEM, ATPASE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="272" mass="31096" checksum="B62B29BC28F7BF87" modified="2017-09-27" version="1">MSKEMLVCDHVTKIFTSGFIKKIEVKAVDDVSFTVREGEIISLIGQSGSGKTTLGKIILRLIPPTSGRVLFYGKDIWKDIKTKEERKEYWRQVHAVFQNPMGSFNEFYRIDRVLDQALELIGADPKSEEGMLLKEESLKAVGLNPDELLGKYPHQLSGGQLQRVMIARSWILRPKLLIADEAVSMLDVSTRGKIVEIFKELRNKLGSSIIFISHDIGLSYFISDRIFIMYKGKIIEEGTPQEVIDNPKHEYTKTLVESVPTIYRKWEDFLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8I7</accession>
    <name>A0A218P8I7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Glucanase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_07090</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="9">Binds 2 divalent metal cations per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase M42 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854352.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8I7"/>
    <dbReference type="GeneID" id="33316021"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07090"/>
    <dbReference type="OrthoDB" id="84932at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05657">
        <property type="entry name" value="M42_glucanase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.40">
        <property type="entry name" value="Peptidase M42, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008007">
        <property type="entry name" value="Peptidase_M42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051464">
        <property type="entry name" value="Peptidase_M42_aminopept"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023367">
        <property type="entry name" value="Peptidase_M42_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32481">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32481:SF7">
        <property type="entry name" value="AMINOPEPTIDASE YHFE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05343">
        <property type="entry name" value="Peptidase_M42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001123">
        <property type="entry name" value="PepA_GA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101821">
        <property type="entry name" value="Aminopeptidase/glucanase lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR001123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ07102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="339" mass="37331" checksum="2B01389467376A2D" modified="2017-09-27" version="1">MERVVEILKEILEVPSPTGYTKEVLAHIEKKLNEAGIKTYYTNKGALIAGNHPEPELVIAGHVDTLGAMVKGILPDGHLSFTRIGGLLLPTFEGEYCTIITRFGKKFRGTLLLKNPSVHVNREAGKKERKEENMYIRLDELVEKREDTEKLGIRPGDFVAFDPKFEYVNGFVKAHFLDDKASVAVMIDLLLELGAEKLEKLPVAFFFSPYEEVGHGGSAGYPPSTKELLVVDMGVVGDGVAGKETAVSIAAKDSSGPYDYEMTTKLIELAEKREIPHVVDVFPYYGSDGSAALRAGWDFRVALIGPGVHASHGMERTHVKGLLATKELIKAYIEDKFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8N4</accession>
    <name>A0A218P8N4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Restriction endonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_07360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854404.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8N4"/>
    <dbReference type="REBASE" id="210321">
        <property type="entry name" value="TpaP4McrBC2P"/>
    </dbReference>
    <dbReference type="GeneID" id="33316075"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07360"/>
    <dbReference type="OrthoDB" id="91413at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019292">
        <property type="entry name" value="McrC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38733">
        <property type="entry name" value="PROTEIN MCRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38733:SF1">
        <property type="entry name" value="TYPE IV METHYL-DIRECTED RESTRICTION ENZYME ECOKMCRBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10117">
        <property type="entry name" value="McrBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="463" mass="53451" checksum="95573DA1E524802E" modified="2017-09-27" version="1">MRAVTIYEFQRKYLQSLHAEGINVSPSELQRFFESINSIYGREGDVLSLRYDVSRGDYYIQAHGSVGFAYYLRDEPFMIQVLPKPYRNDPDHRRSMKFFLSLLNLSGNLGMSQRDIERAVSAYRGEDELIHEMFFYLYTSQLGRKIFHGMYREYSEVEENSKTIRGRVLLSRLIRKTPGSLDIPIRHTVLGIDNPLNRVLKAALEVVAENSSWEGVRREASALLEYFREVGRLKDDDPGRVSFNSLNERFRPVYTLAMIVLEGFGRGQQRGLVQHGIFMDMAHLFETLVYHTIRRVLQGEATVYRDYRLPHIVEGAQKVEAKLGAVFTFGPPLPDILVATDYGKCVIEVKYRSLLVKMMGQWRRKLVRTSEELYQIYSYSRLAGGSAAVVYPRLEGVYNYWIPDMFGPEENILSFFDGTPFGLFGYELSRVGREVFITRNGVVLREGVARNLREHLLSLCMGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8N5</accession>
    <name>A0A218P8N5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA-processing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_07330</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854398.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8N5"/>
    <dbReference type="GeneID" id="33316069"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07330"/>
    <dbReference type="OrthoDB" id="7870at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22389">
        <property type="entry name" value="KH-I_Dim2p_like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22390">
        <property type="entry name" value="KH-I_Dim2p_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000076">
        <property type="entry name" value="KH domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000119">
        <property type="entry name" value="KH type 1 domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019964">
        <property type="entry name" value="KH_domain_protein_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055211">
        <property type="entry name" value="KH_PNO1_2nd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03665">
        <property type="entry name" value="arCOG04150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010330">
        <property type="entry name" value="PRK13763.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826">
        <property type="entry name" value="RIBONUCLEASE Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826:SF13">
        <property type="entry name" value="RNA-BINDING PROTEIN PNO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22891">
        <property type="entry name" value="KH_PNO1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="35"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="126"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="234"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="244" mass="27676" checksum="4096F3A4B8346C19" modified="2017-09-27" version="1">MDEFERLLKKYERVDKDGGPIREEPEVEIDYAAEGEQEEFLRIPKERVAVVIGKKGQTKREIERRTKTKIEIDSETGEVFITSTEKTDDPLAVWKARDVVMAIGRGFSPERAFRLFNEGETLEVVNLTDIIIGNDKNALPRVRGRIIGRKGRTREIIEEMSGADVSVYGKTVAIIGNPLQVEVAKTAIEKLAKGSPHGVVYKYLERRKKDLELESSNYYEALEGGAGEAGDYPEFSEDDEYWED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8T9</accession>
    <name>A0A218P8T9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">LTD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_250638360.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8T9"/>
    <dbReference type="GeneID" id="69102014"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07710"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1260">
        <property type="entry name" value="Lamin Tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001322">
        <property type="entry name" value="Lamin_tail_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036415">
        <property type="entry name" value="Lamin_tail_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00932">
        <property type="entry name" value="LTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74853">
        <property type="entry name" value="Lamin A/C globular tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51841">
        <property type="entry name" value="LTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LTD" evidence="1">
        <location>
            <begin position="39"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="159" mass="17961" checksum="03A97B952E8A3CD6" modified="2017-09-27" version="1">MYTEGEFKKEQRYVSYQKSAESKKLGLWGCEFGKYADVNGVATHVASTGDVVIVKVHYDAGGPNVRDTEVLNDEYVVLKNKGSTPVNLKGWFIEDEADHRYYFPSITLQPGKEIVLHTGKGTDTAHDLYWGSGRAIWNNDHDTAYLYNSDGKLVDKFSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8V2</accession>
    <name>A0A218P8V2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Valine--tRNA ligase</fullName>
            <ecNumber evidence="11">6.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Valyl-tRNA synthetase</fullName>
            <shortName evidence="11">ValRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">valS</name>
        <name evidence="14" type="ORF">A3L08_07660</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 11">Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 11">
            <text>tRNA(Val) + L-valine + ATP = L-valyl-tRNA(Val) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10 11">Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854453.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8V2"/>
    <dbReference type="GeneID" id="33316135"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07660"/>
    <dbReference type="OrthoDB" id="23906at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00817">
        <property type="entry name" value="ValRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.720.200:FF:000001">
        <property type="entry name" value="Glycine--tRNA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000033">
        <property type="entry name" value="Valine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000192">
        <property type="entry name" value="Valine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02005">
        <property type="entry name" value="Val_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022874">
        <property type="entry name" value="Valine-tRNA_ligase_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009687">
        <property type="entry name" value="PRK13208.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00422">
        <property type="entry name" value="valS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946:SF93">
        <property type="entry name" value="VALINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="VALYL-TRNA SYNTHETASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19302">
        <property type="entry name" value="DUF5915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00986">
        <property type="entry name" value="TRNASYNTHVAL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 11 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 11 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="13">
        <location>
            <begin position="596"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="11">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="11">
        <location>
            <begin position="536"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="539"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ07203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="890" mass="103828" checksum="3A03B5D02FF97111" modified="2017-09-27" version="1">MLPKNYDPNEIEPKWQKFWLDEKIYKYELDEKRPAYAIDTPPPFTSGTLHLGHVLSHTWIDIIARYKRMTGYNVLFPQGFDNHGLPTELKVEKEFGISKDQPERFLQKCIEWTWQAIEAMRNQFIRIGYSADWDLEYHTMDDWYKAAVQKSLLEFYKKGMLYRDEHPVYWCPRCRTSLAKAEVGYVEEDGYLYYIKLPLADGSGHVPIATTRPELMPACVAVFVHPDDERYKDVVGKKVKLPIFEREVPVLADEDVDPTFGTGAVYNCTYGDEQDVVWQKRYNLPVIIAINEDGTMNERAGPYKGLKTEEARKKITEDLENMGLLYDRKKVHHRVLRHTERSSCMAPIELLPKKQWFIKVKDFTDEIVKVAEQINWYPEDMFLRLKDWAESMDWDWVISRQRVFGTPIPFWVCKNGHVVPAKEEDLPVDPRFDKPPVEKCPLCGADLEPVTDVLDCWVDSSITPLIISRWHEAIKGNEEAKRWFEHNFPTALRPQGTDIIRTWAFYTIFRTWVLTGEKPWHDILINGMVAGPDGRKMSKSYGNVVAPDEVIPKYGADALRLWTALAPPGEDHPFKWETVDYNYRFLQKVWNIYRFAERHLEDFDPANAPSELEPLDRWILSRLHRLIKFATEEMERYRFNLLTRELITFVWHEVADDYIEMIKYRLYGDDEESKLKAKAALYELLYNIMLLLAPFVPHITEELYQEMFKKHVGAKSVHLLEWPKYDEGKIDEKAEKLGELAREIVGAMRRYKNGHGLALNAKLKHVAIYATDSYEMLKTIEKDIAGTMNIERLEIIKGEPELEERIIEIKPNFRTVGPRYGKLVPKITAYLKDNAEEVAKALKESGKVEFEVDGQKVELTKDDIVLRKAVFSEGEEVETAVVGDAVVLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8W2</accession>
    <name>A0A218P8W2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TldD/PmbA family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_07725</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854462.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8W2"/>
    <dbReference type="GeneID" id="33316147"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07725"/>
    <dbReference type="OrthoDB" id="84520at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="entry name" value="PmbA/TldD superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047657">
        <property type="entry name" value="PmbA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43421">
        <property type="entry name" value="METALLOPROTEASE PMBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43421:SF1">
        <property type="entry name" value="METALLOPROTEASE PMBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="Putative modulator of DNA gyrase, PmbA/TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloprotease TldD/E N-terminal" evidence="1">
        <location>
            <begin position="16"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloprotease TldD/E central" evidence="3">
        <location>
            <begin position="107"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloprotease TldD/E C-terminal" evidence="2">
        <location>
            <begin position="218"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="441" mass="48553" checksum="25100DA04E1DD5A5" modified="2017-09-27" version="1">MENLIRFGEKLFDELEIAVYRSRDVSASVELNEISMASTRSGAVTVIRGIKDKRLGLAIVDSDEPERIKEAIEQAAKMAKLNSSDEKWVSLPEPGKYREKPKPNYELKETSPDILVEKLVHGIKLAREKDPNAVVAGGEGGVSWEERRILNSHGIDIFQEGGAAFFYLELVGRKGDVVTPGIFDFDARRDLNLDVDGVVERAVQKVGWAYNVEKSRNEEVPIILGPWAIAGLFSYALLPAFSGERLVKETTPLAGKVGEMIASEVLTIYDDPFHPLSLEPVIADGEGVPTRKNILIEEGTFKGFVWDNYWAKVYGTESTGNGKRDLRSGGINIGFHSVVIENGKRSLEDLIAEIDRGYLVDGLQGAHSSNPDNGNFAVTANPAFLIEDGEVKGASVFLIAGNVYELLKQTSEVTKEQTVMPFMNTMITPHIKFENVKIAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8X0</accession>
    <name>A0A218P8X0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 17 18">Pyruvate kinase</fullName>
            <ecNumber evidence="6 17 18">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">A3L08_07805</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 18">
            <text>pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 18">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the pyruvate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="6 17 18"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854477.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8X0"/>
    <dbReference type="GeneID" id="33316164"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07805"/>
    <dbReference type="OrthoDB" id="56298at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.33.10:FF:000001">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1380.20:FF:000013">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="entry name" value="Pyruvate kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004491">
        <property type="entry name" value="PRK05826.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004978">
        <property type="entry name" value="PRK06354.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="PK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14 18" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 18 21" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0630">Potassium</keyword>
    <keyword evidence="15 21" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="19">
        <location>
            <begin position="6"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate kinase C-terminal" evidence="20">
        <location>
            <begin position="368"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="NCBIfam" id="TIGR01064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ASJ07229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="478" mass="53105" checksum="97B1FBEA5ED90DD4" modified="2017-09-27" version="1">MRLPSHKTKIIATIGPSSLKRETIEAMIKAGMSVARINFAHGDLEQHARTIELVRSVSERLNRPVAILGDLPGVKIRVGEIAGGSVVLRRWQTVTLTTRDVVGNEGVIPVQFKDFPKLVSKGDIIYLNDGFIALRVEEVREPDVICKVLVGGTLFSHKGINVPRARMAIDAVTEKDLEFIEFALEHGIDAVGISFVGSAYDVLKVRRFVEERNARMFIVAKIERPDAVKNFDEILNAADGIMIARGDLGVEMPIERLPILQKKLIHKANCAGKPVITATQMLESMTEEKLPTRAEVTDVANAILDGTDAVMLSEETAVGKYPVDAVRMMARIAKTTEAYRDSLWSARIVELKMSEWKGRVQRKGTIKDAIARSIIEALNSIDIKYILTPTRTGETARLISRFKPKQWILAFVTDEWVGNTLMFSYGVYPFTVEETSEEEILRVIRSLGLVKENDTVLLTKGTPIGKIVGTNTIRIFTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8X1</accession>
    <name>A0A218P8X1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">A3L08_07585</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. BUD32 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854441.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8X1"/>
    <dbReference type="GeneID" id="33316120"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07585"/>
    <dbReference type="OrthoDB" id="31344at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000951">
        <property type="entry name" value="EKC/KEOPS complex subunit BUD32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000201">
        <property type="entry name" value="TP53-regulating kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022495">
        <property type="entry name" value="Bud32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03724">
        <property type="entry name" value="arch_bud32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011462">
        <property type="entry name" value="PRK14879.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011463">
        <property type="entry name" value="PRK14879.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011464">
        <property type="entry name" value="PRK14879.1-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209:SF0">
        <property type="entry name" value="EKC_KEOPS COMPLEX SUBUNIT TP53RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209">
        <property type="entry name" value="NON-SPECIFIC SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ07190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="222" mass="25888" checksum="8472C3CB6CB8C61B" modified="2017-09-27" version="1">MELIKQGAEAKIYLAGFAEFFGADLLPGEKVIVKHRIPKRYRIEEIDRKLRKERTVREARVLHRAKEFGVNCPYVYEVDLKDMKIVMEFIEGDRLKELLESVPMEERLKLCREVGRQIGKLHEAGIVHGDLTTSNMILREGKVYLIDFGLADFDTTLEAQGVDLHLLRRAMESTHYTWFEAGFEAVLEGYAEIRGKKAREEIEEKLEEIESRGRYRERSWVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P909</accession>
    <name>A0A218P909_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_07960</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854507.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P909"/>
    <dbReference type="GeneID" id="33316197"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07960"/>
    <dbReference type="OrthoDB" id="23466at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02766">
        <property type="entry name" value="MopB_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.90">
        <property type="entry name" value="ADC-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.228.10">
        <property type="entry name" value="Dimethylsulfoxide Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2070.10">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006655">
        <property type="entry name" value="Mopterin_OxRdtase_prok_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050612">
        <property type="entry name" value="Prok_Mopterin_Oxidored"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43742:SF6">
        <property type="entry name" value="OXIDOREDUCTASE YYAE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43742">
        <property type="entry name" value="TRIMETHYLAMINE-N-OXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04879">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53706">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase, domains 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00932">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="8">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="636" mass="72974" checksum="09698CEABA936FB1" modified="2017-09-27" version="1">MPFSVCMRDCYDTCSMISEFKDGKLTVRGNPRHPVTAGFLCPKGALLPKWFHAKGRLKKPLIRTDERGSGEFRETSWEEAIKVVVDKLRETIEEHGSESVLVYQYAGDRGAVNYAFPLRLFHYLNTSMLDYGICDRAGHEALKDVYGTAVGMDPEELKNQRLLVYWGMNAFWTNLHGFMLAKKNNLEIWTVDVVRTETAKRSDRFFQIRPDTDVLFALSVAKVIIEENLYDKAFVRENVYGFEEFKNYVKTLSLDYVSKETGLSIEDIEEFARGYAEKRGVIHIGYGFQRSLAGGEAVRAIAILPALVGHRFGFIYDMKTIDKSYAEGAFLRSKPAKRIPQVKLAEYIERGEIKFLYVYNSNPLASLPNQNRLRKALVESDVFVVTHDIFLTDTALYSDVVLPANTFFERLDIADSYYHRYVALNEPVARLHGKSNSEVTRLLAKALGIENPYLYESDEEVIRKILELNGLSWDELKEKGFVRVPEKPRKWETPSGKIEFYSQRVVERGLSPFPEYRKFKGKYPLRLLTPTYRMTITSQYHNTYGMVDPNLYINPADAEERGIQDGDAVEVFNENGRIRTVAKLSDDVPRGVVLLYKAFWVKLLGWNANFLTIDETVQNYGNGSAYHSTWVDVRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P912</accession>
    <name>A0A218P912_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Beta-glucosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_08105</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854532.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P912"/>
    <dbReference type="GeneID" id="33316226"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08105"/>
    <dbReference type="OrthoDB" id="84443at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018120">
        <property type="entry name" value="Glyco_hydro_1_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF209">
        <property type="entry name" value="GALACTOLIPID GALACTOSYLTRANSFERASE SFR2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00572">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="404" mass="47250" checksum="CBC6F6BA68E171C5" modified="2017-09-27" version="1">MRKFLFGTSTAAYQIEGDNRWSDWWYWSEKGRLPKAGKACNHWEKYEEDIKLMAELGYNAYRFSIEWGRIFPEEDKINEEALARYREIIELLLKSKIDPMVTLHHFTLPTWFALKGGFARDENLKYWERYLNAVSDILNGVRLIATTNEPMELVIEGYLTGNWPPFLRDPKTAFQVEKNLINAHSIAYETLSGKHEVGIVKSMPSIKFPDGEVAEEVENLQTFYFLDAIFEGVLKTPLGDMRVLPSSMDFIGVNYYTLHIVSKDTEPLRGLYEYEFGGYGRTQMGWKIYPRGIYEAIVKASKYGKPIYITENGIATENEDERIKFIQDHLNWVKKAMEEGYDVRGYFYWSFIDNYEWDKGFVPKFGLVSFNPETWERIPKKSAFAYGRLAKNFPYQGRETCPKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P913</accession>
    <name>A0A218P913_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF302 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P913"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08085"/>
    <dbReference type="OrthoDB" id="2559at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd14797">
        <property type="entry name" value="DUF302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.70">
        <property type="entry name" value="TT1751-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005180">
        <property type="entry name" value="DUF302"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035923">
        <property type="entry name" value="TT1751-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03625">
        <property type="entry name" value="DUF302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103247">
        <property type="entry name" value="TT1751-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF302" evidence="1">
        <location>
            <begin position="49"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="125" mass="14290" checksum="9CB99E3E29DEB1FC" modified="2017-09-27" version="1">MLLFKKVYAVEKREERLDYLKKRLEEEGFLLIGERIPVAIVGREDGIVADYHLLFICHSELVEELIRIDPNIGALLPCTGFGYRREDGNYLGVTLPSVAWKIAGEDVARLMKPMEERVIKIVNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P957</accession>
    <name>A0A218P957_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rubrerythrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854562.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P957"/>
    <dbReference type="GeneID" id="33316260"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08270"/>
    <dbReference type="OrthoDB" id="91068at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01045">
        <property type="entry name" value="Ferritin_like_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rr_diiron-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33531:SF10">
        <property type="entry name" value="BLR7895 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33531">
        <property type="entry name" value="RUBRERYTHRIN SUBFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rubrerythrin diiron-binding" evidence="1">
        <location>
            <begin position="22"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="174" mass="20562" checksum="37A3BB29E3C79528" modified="2017-09-27" version="1">MVPPELEEGLPLERIRDFSLEELLGMAIKAEIGSRKFYESLAERIDIQELKNKIEWLAGEEKKHEELLRKIYANMFPGKEIVFPNEHIGPELQPVARQLHGVEDIIDLIRWAMKAEEIAAKFYAELEKMVDTEEKKRLMRYLSDMEWGHYYNLKAEYELLLDWAMYSQMMNVGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P961</accession>
    <name>A0A218P961_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_08355</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854578.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P961"/>
    <dbReference type="GeneID" id="33316277"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08355"/>
    <dbReference type="OrthoDB" id="44250at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051782">
        <property type="entry name" value="ABC_Transporter_VariousFunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42939">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN ALBC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42939:SF1">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN ALBC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="231" mass="25095" checksum="AA303BE4F44CC959" modified="2017-09-27" version="1">MTVIRAENLHKYFGPIKALQGVTVEIPEGLTLILGPNGGGKSTFMKVALGLYRPTKGSVRLLGKDPWKSPEARKGVGVAFDPGRFPKLTTGREWLEFIARGRGASAGDVEKAAGLFGIEDALDRRIEGYSSGMLKRLSLAQAFVGEPDVIFLDEPLANVDFESVAEIVGVIGKWKGWGKSFVIISHLWEPFENLADYGVVISGGKVYLEGPFVDIRDDVEAMFRPPSVATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P980</accession>
    <name>A0A218P980_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854579.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P980"/>
    <dbReference type="GeneID" id="33316278"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08360"/>
    <dbReference type="OrthoDB" id="95217at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="145" mass="15573" checksum="8FD66B3BBFD435C1" modified="2017-09-27" version="1">MRLFRVLLLTLMLVSLLSLGLQSGLEPSDVHYHNAFVAGREATAVYISSTSSAKLYAVGIGEFRVRDVQTDEVIFSGRVNGNGAFAVVFPHPGFYEFSGNSSQGITITITPVDVGFPGKQKTMHLWIAVLFGGLLAVTLLGGGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9C0</accession>
    <name>A0A218P9C0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA/RNA-binding protein Alba</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">albA</name>
        <name evidence="8" type="ORF">A3L08_08570</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Binds double-stranded DNA tightly but without sequence specificity. Involved in DNA compaction.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="6">Acetylated. Acetylation at Lys-11 decreases DNA-binding affinity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the histone-like Alba family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010477671.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9C0"/>
    <dbReference type="GeneID" id="27134161"/>
    <dbReference type="GeneID" id="7988446"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08570"/>
    <dbReference type="OrthoDB" id="10360at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01122">
        <property type="entry name" value="AlbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013795">
        <property type="entry name" value="DNA/RNA-bd_Alba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00285">
        <property type="entry name" value="DNA-binding protein Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003088">
        <property type="entry name" value="PRK04015.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028732">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="AlbA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2 6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA-binding protein Alba-like" evidence="7">
        <location>
            <begin position="5"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine" evidence="6">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="91" mass="10106" checksum="350E45C4FEF86D5C" modified="2017-09-27" version="1">MAEEHVVYIGKKPVMNYVLAVITQFNEGAKEVTVKARGRAISRAVDVAEIVRNRFLPEVRVKEIKIGTEELPTADGRTANTSTIEIVLEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9C6</accession>
    <name>A0A218P9C6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_08705</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9C6"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08705"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00397">
        <property type="entry name" value="DNA_BRE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="2">
        <location>
            <begin position="33"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="163" mass="18887" checksum="9B85565C167B9AE1" modified="2017-09-27" version="1">MVLRNFGMPESLIDVLKRPKREANIARRIEQEGNTPSLDIEDAREFFKRLEMPFQLGELNETQYIKAVAFALLLFATGRRVSEIVQVRAQDIDFKTHTIRILVSQTKEGKIQKITSGERIVFVTKETEAVLRFYLEINKKEIEGQDGYLFMTPGKRSLKDTCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9D1</accession>
    <name>A0A218P9D1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Large helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">A3L08_08640</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="9">Belongs to the Lhr helicase family. Lhr-Core subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854626.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9D1"/>
    <dbReference type="GeneID" id="33316334"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08640"/>
    <dbReference type="OrthoDB" id="33870at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052511">
        <property type="entry name" value="ATP-dep_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017170">
        <property type="entry name" value="Lhr-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013701">
        <property type="entry name" value="Lhr-like_DEAD/DEAH_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045628">
        <property type="entry name" value="Lhr_WH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962:SF5">
        <property type="entry name" value="ATP-DEPENDENT HELICASE LHR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962">
        <property type="entry name" value="ATP-DEPENDENT HELICASE LHR-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08494">
        <property type="entry name" value="DEAD_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19306">
        <property type="entry name" value="WHD_Lhr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037307">
        <property type="entry name" value="Lhr-like_helic_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="24"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="233"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00093467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ07379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="928" mass="106344" checksum="424BB7E55369ACC0" modified="2017-09-27" version="1">MHLLLKKAIKERFGRLNRLQQDAFKEVSSEKSVLIIAPTGSGKTEAAVLPVFNEILERGLKPISALYIAPLKALNRDLLDRLEWWGRKLGISVEVRHGDTSAYRKAKQTKSPPQMLIITPETLGVILTVKSLRKHLENVKFVIVDEIAELVDNKRGAQLLLNLERLAEIAEFKRIGMTATVGNEEEVREWLKADVIVKPPWKKNYRFHVLYPSPSEEDEELARKLSLSPEIAARLRLLWEIVEKHGKALIFTNTRQFAEILAHRLKAWGKPVEVHHGSLSKEARIAAERALKEGKIRALICTSSMELGIDIGDVDVVIQYMSPRQVNRLVQRAGRAKHRIGEVSEAYVITSNVEDYLQSLVIAKHALEGRFEAVEPMGGLDVLAHFIVGLLIEYKRLPRERPYEIARRAYVYRDLSWEDYLDVLRVLEDARLVGYDEETGLLYLRRGAFQYYYENLSTIPDEVSWRVFDAGGGHIIGRLDESFVMDLEEGMEFVMNGRSWIVLTIDDEARLLKVRESRSMESAIPSWEGEMIPVPFGVALAVGRLKRELAFDFRKAKELLEGVEFREEELKRAFEEIKGEPFSTDRDIVVESTPKALVIHADFGNRANEALGRLVHSFLILRYGRVFSVRAQAHAVVFKTPFQLNPEEVKGYLYQEPESLEFIVARALRDSHAYRWRMLNVAKRFGALRRDAKIRRVERLFEGTIIERETLNELYHDKVDVSKGKLVLEMLKRGTMRVKSELRREPSTLARLNMTVGGEFLLTGVLERDEILELFRNRLLDHEVVLVCTNCGWHSKTKVSRLRSIRDRECPRCGSKMLAVAHPIDAEDFLPVLEKVRHGKPLERKEERVYRKLLKAADLVDSYGFEAVLALASYGTGPDTAARLLAQYRGDALLVALMEREREFIRTRRFWVEGKEKDGEEGEKAGES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9D2</accession>
    <name>A0A218P9D2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0248 protein A3L08_08720</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_08720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0248 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854640.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9D2"/>
    <dbReference type="GeneID" id="33316350"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08720"/>
    <dbReference type="OrthoDB" id="14794at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01245">
        <property type="entry name" value="UPF0248"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040459">
        <property type="entry name" value="MJ1316"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007547">
        <property type="entry name" value="UPF0248"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003272">
        <property type="entry name" value="PRK04257.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04457">
        <property type="entry name" value="MJ1316"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MJ1316 RNA cyclic group end recognition" evidence="2">
        <location>
            <begin position="6"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="81" mass="9561" checksum="EB9A614212BC2DA0" modified="2017-09-27" version="1">MRKGFVKEVLAKIKYDPRENEGDYYVVIEHRGAYGDVKRIPVEVIELGHGYFFVGDAQIPYHRILKVVRKDGRVIWETRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9E5</accession>
    <name>A0A218P9E5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Small ribosomal subunit protein eS28</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rps28e</name>
        <name evidence="6" type="ORF">A3L08_08810</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eS28 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854657.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9E5"/>
    <dbReference type="GeneID" id="33316368"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08810"/>
    <dbReference type="OrthoDB" id="7620at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000145">
        <property type="entry name" value="30S ribosomal protein S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00292">
        <property type="entry name" value="Ribosomal_eS28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000289">
        <property type="entry name" value="Ribosomal_eS28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028626">
        <property type="entry name" value="Ribosomal_eS28_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003080">
        <property type="entry name" value="PRK04007.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01200">
        <property type="entry name" value="Ribosomal_S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00961">
        <property type="entry name" value="RIBOSOMAL_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="70" mass="7910" checksum="34470355870165BF" modified="2017-09-27" version="1">MSDEGFPAEVIEIVGRTGVTGGVTQVKVRVLEGRDKGRVIRRNIKGPVRVGDIVILRETEREAREIKRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9I5</accession>
    <name>A0A218P9I5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PadR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854695.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9I5"/>
    <dbReference type="GeneID" id="33316411"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09025"/>
    <dbReference type="OrthoDB" id="56053at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43252">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR YQJI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43252:SF5">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, PADR-LIKE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator PadR N-terminal" evidence="1">
        <location>
            <begin position="14"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="158" mass="18244" checksum="9C9C3828EC7A65FF" modified="2017-09-27" version="1">MERPHFKGHLKLFILKILAERPMHGYGIMAELERTYGIPHPSPGTVYPILTSLRKAGLIETVGEGKRDKRLYRATEKGKEYLREHEVELREGEELAYRFREFARLGGRELAEVMKEAFNSIDDLTEAQKKALAREFGEFTKRVRLILLGEIERGEGSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9J0</accession>
    <name>A0A218P9J0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Bifunctional biotin--[acetyl-CoA-carboxylase] synthetase/biotin operon repressor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_08925</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9J0"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08925"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin--[biotin carboxyl-carrier protein] ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004408">
        <property type="entry name" value="Biotin_CoA_COase_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013196">
        <property type="entry name" value="HTH_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00121">
        <property type="entry name" value="birA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835">
        <property type="entry name" value="BIOTIN PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835:SF5">
        <property type="entry name" value="BIOTIN--PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08279">
        <property type="entry name" value="HTH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="2">
        <location>
            <begin position="61"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="244" mass="27058" checksum="2BA2B3EF021B958D" modified="2017-09-27" version="1">MVRDSRIKRAILRELREKECVSGEFLAESLGVSRVAVWKNIRELISLGYEIRASRKGYSLVSSPEKPYPWELDVRAYYLLETTSTMDIAWKLAEAEEPAGTFVIAERQTRGRGRRGRRWRSEKGGLYFSVILKPALPLSRADVLLDETLDSILDSLRRYGVDGYIEDNGVYVDGKKIAGVLVEVMGELGEVRLAVVGAGINVSNEVPEGATSLSLELGRAPSLLEVGKTLFPTVKGRLLSVSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9J8</accession>
    <name>A0A218P9J8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 14 17">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="6 14 17">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">pgk</name>
        <name evidence="18" type="ORF">A3L08_09010</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14 17">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14 17">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="6 14 17"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854692.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9J8"/>
    <dbReference type="GeneID" id="33316408"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09010"/>
    <dbReference type="OrthoDB" id="6575at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00185"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000006">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000012">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="entry name" value="Phosphoglycerate kinase, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015911">
        <property type="entry name" value="Phosphoglycerate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406:SF23">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00111">
        <property type="entry name" value="PGLYCERATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 14 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 14" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="11 14 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14 17" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="14 15">
        <location>
            <begin position="19"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 15">
        <location>
            <begin position="57"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 16">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="358"/>
            <end position="361"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00016471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ASJ07448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="412" mass="46313" checksum="C2213E60F47068A5" modified="2017-09-27" version="1">MFRLTDFNFHNKTVFLRADLNSPVKDGRIISDARFRAVLPTVSYLLEHGAKVIIGTHQSKPYKGDYTTTEEHAEILSELLGQDVEYVEDIFGRYARERIRELKPGEALMLENLRFAAEEVKYKPIEDCEKTFFVRKLAPLIDYVVNDAFAAAHRSQPSLVGFARLKPMIMGFLMEKEVEALARAYDTEERPRAYVLGGAKVDDSLRVAENVLRNGRADLILTGGLVGQVFTLAKGFNLGDANIEFMEKKDLLELVDWAEKILDDFYPYVRTPVDFAVDKDGERVEVDLLSGEKWLFDRYPILDIGSRTVEKYRDILMKAKVIVANGPMGVFEREEFALGTVGVFRAIGESPAFSVVGGGHSIASIYQHNITGISHVSTGGGAMLSFFAGEKLPVIEAFRISYERFKDKIKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9P3</accession>
    <name>A0A218P9P3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09400</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854760.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9P3"/>
    <dbReference type="GeneID" id="33316486"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09400"/>
    <dbReference type="OrthoDB" id="84899at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="140" mass="15964" checksum="1145378186749F32" modified="2017-09-27" version="1">MPVLGINITDVRINKSSTVSPTGQIEVKLTPKIKELRLGEIRLPTGKTNGIEVLFSYNIQYTPAIAEGSIEGLLMYLPPQKENLDEILDQWEKEKRLDPFMFAEVVNFITKEVSPMLMLLAKEMRLPYHIPIPRVEVKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9P7</accession>
    <name>A0A218P9P7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dTTP/UTP pyrophosphatase</fullName>
            <shortName evidence="3">dTTPase/UTPase</shortName>
            <ecNumber evidence="3">3.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Nucleoside triphosphate pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Nucleotide pyrophosphatase</fullName>
            <shortName evidence="3">Nucleotide PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_09345</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Nucleoside triphosphate pyrophosphatase that hydrolyzes dTTP and UTP. May have a dual role in cell division arrest and in preventing the incorporation of modified nucleotides into cellular nucleic acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>UTP + H2O = UMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29395"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="EC" id="3.6.1.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>dTTP + H2O = dTMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28534"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37568"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="EC" id="3.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Maf family. YhdE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.9" evidence="3"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854751.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9P7"/>
    <dbReference type="GeneID" id="33316475"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09345"/>
    <dbReference type="OrthoDB" id="45223at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036218">
        <property type="term" value="F:dTTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036221">
        <property type="term" value="F:UTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00555">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00528">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003697">
        <property type="entry name" value="Maf-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00172">
        <property type="entry name" value="maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213">
        <property type="entry name" value="BIFUNCTIONAL DTTP/UTP PYROPHOSPHATASE/METHYLTRANSFERASE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213:SF5">
        <property type="entry name" value="BIFUNCTIONAL DTTP_UTP PYROPHOSPHATASE_METHYLTRANSFERASE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02545">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006305">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="3">
        <location>
            <position position="10"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="3">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="3">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="184" mass="20591" checksum="4CCC29E69BC8D862" modified="2017-09-27" version="1">MLVLASASPRRREILGRFIREFEVVPSEVDEHCSLDDPREYALELARRKARDVYSRVGGTVIGADTVVSINGQILGKPKDEGHAFRMLRLLSGRVHQVTTGYCIIHGGKEHCGAVVTDVKFRELDDEVIRAYVATGEPMDKAGAYGIQGKGGLFVEWIRGDYYNVVGFPLEIVWKLRELGFRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9S6</accession>
    <name>A0A218P9S6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Molybdopterin-guanine dinucleotide biosynthesis protein MobB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_09550</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854789.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9S6"/>
    <dbReference type="GeneID" id="33316516"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09550"/>
    <dbReference type="OrthoDB" id="9014at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.15.40">
        <property type="entry name" value="Electron transport complex subunit B, putative Fe-S cluster"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007202">
        <property type="entry name" value="4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052539">
        <property type="entry name" value="MGD_biosynthesis_adapter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004435">
        <property type="entry name" value="MobB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00176">
        <property type="entry name" value="mobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011064">
        <property type="entry name" value="PRK14494.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40072:SF1">
        <property type="entry name" value="MOLYBDOPTERIN-GUANINE DINUCLEOTIDE BIOSYNTHESIS ADAPTER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40072">
        <property type="entry name" value="MOLYBDOPTERIN-GUANINE DINUCLEOTIDE BIOSYNTHESIS ADAPTER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04060">
        <property type="entry name" value="FeS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03205">
        <property type="entry name" value="MobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51656">
        <property type="entry name" value="4FE4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S" evidence="5">
        <location>
            <begin position="148"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="244" mass="26974" checksum="847B856F84633F2A" modified="2017-09-27" version="1">MRAVAFVGVKKSGKTTTVEAVARVLRERGYRVAIAKSMHAEFDREGTDTWRFSQVADSVIVRANDTDALLFKAKDINALFSMVNADFLLLEGFKSVQHVPKVICAKSEEDVRELNDGLAVAVSGVVAGNEVREIDGLPVIDATKEPEKLADLVEKRAFMLPNIDCGLCGFKCAEMARMIVKGEKTLRDCVVLSSKPKVTVKIDGQVLPMKDWVQELVEKTIKGMLSAMKGYRDGRKIEIVIRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9U1</accession>
    <name>A0A218P9U1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09640</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854805.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9U1"/>
    <dbReference type="GeneID" id="33316534"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09640"/>
    <dbReference type="OrthoDB" id="95899at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="327" mass="35729" checksum="C742A039ECD6067A" modified="2017-09-27" version="1">MRKILAFILVAVVIASGCLEDSDNNATSDATPTTISSETGFLEELGKVSPSDPLCGMNPNVSVSHFIDPLREDYSISPAEPEFPVPGGSLAGQTLEVSPKGVSSKVYVSVFAYPDSYSATEALAELKEKLNRLGNSTDEFKWEGKNALYSAFTTYFGGFYLIVVFQVPYGEDGLNMAEYALNEVMFHVMWVGPSPEKMLGLFMHTEIVNKTGRSRVFSGDLLSESGFTPEGARIEVAVYREGCGQEVYRELKSKIEKIGFTEKSLPPLNSRDPAGKVVERTYFEGNGGVYIELYDGPGMDRVMLLYGNESAVKAAVQKIWSGEGTFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9U4</accession>
    <name>A0A218P9U4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Methionine--tRNA ligase</fullName>
            <ecNumber evidence="4">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Methionyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">A3L08_09605</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="4"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854799.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9U4"/>
    <dbReference type="GeneID" id="33316527"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09605"/>
    <dbReference type="OrthoDB" id="30609at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02800">
        <property type="entry name" value="tRNA_bind_EcMetRS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000042">
        <property type="entry name" value="Methionine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004495">
        <property type="entry name" value="Met-tRNA-synth_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051270">
        <property type="entry name" value="Tyrosine-tRNA_ligase_regulator"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00399">
        <property type="entry name" value="metG_C_term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586">
        <property type="entry name" value="TRNA-AMINOACYLATION COFACTOR ARC1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586:SF37">
        <property type="entry name" value="TRNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 18" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 16" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 16" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="17">
        <location>
            <begin position="8"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ASJ07555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="109" mass="12520" checksum="014CBBDE2835A505" modified="2017-09-27" version="1">MELYDVEEFWKFDIRVGLVKKAEKLKRTRKLIKLEVDFGSEERTIITGIADQYSPEDLEGKKFVFVLNLKPKKLSGVESRGMLIVAETEDGTVYLLPVSEEVPVGTRVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9W5</accession>
    <name>A0A218P9W5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">A3L08_09650</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854807.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9W5"/>
    <dbReference type="GeneID" id="33316536"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09650"/>
    <dbReference type="OrthoDB" id="121502at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18542">
        <property type="entry name" value="ABC_6TM_YknU_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000299">
        <property type="entry name" value="ABC transporter ATP-binding protein/permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF1">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B MEMBER 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="24"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="339"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ07563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="578" mass="63752" checksum="2CCC54690C950AC7" modified="2017-09-27" version="1">MGESLKGLARLLGYLRGHALHFSLGMVMVLLMSYTSGVVPVLIREAIDRGVTAGDYGQALRYALLILLVGVLNGAFSFAGRYLLVKSAQHAVYHLRMDAFRAIQRQRMEFFDKTFSGQLISRITNDTERITRFLSFRLRMFVYSLFLIAVSLYYMVRMNTLLTAVALVTIGIVVALNSAYAMKVRPIYDRIRHQTGVIASTATGTIAGIKTIKALSVEEEISKKFERENEELYSLNVEATRVVAIYGNASFLVLGIAMSAMLYFGGRAIIVNTLTVGELVAFLTYMLTMTWPLRALGFTIADIQRSLAAASRLFEVVDSAPGSVDPPNAVELKNPRGEVEFRDVWLTYHTGKTVLRGLSFKVKPGEKVVITGPPGSGKSTVLKLIARFYEPEKGEVLLDGVDVRKIKTESLRKTVAYVPQEPFIFNRSIRENIALAKPDATMEEIVKAAKIAKIHDFIVSLPEGYDTVVGEKGVTLSGGQRQRIALARALLLDPKVILLDDPVSNLDAGTEKQLVEDLKEILKDRTALIVSQRPSLVKLADRVIVMAEGRIVEDGKPEELARKGGAFSEMLRAVGGNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9W7</accession>
    <name>A0A218P9W7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854822.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9W7"/>
    <dbReference type="GeneID" id="33316552"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09730"/>
    <dbReference type="OrthoDB" id="84545at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.30">
        <property type="entry name" value="Hypothetical protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007374">
        <property type="entry name" value="ASCH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016645">
        <property type="entry name" value="UCP016134"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04266">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016134">
        <property type="entry name" value="UCP016134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01022">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ASCH" evidence="1">
        <location>
            <begin position="6"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="111" mass="13318" checksum="73C86CE2ED891193" modified="2017-09-27" version="1">MRVYRLRVRDEYLDYIKSGEKRIEVRVAYPQLKGIKPGDKIIFNDEVPAVVTGVKPYETFRQVLREEPIKKIFPDEPSFERAVKRFHNMYPKWKENRYGVIAIRFKLVGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9X8</accession>
    <name>A0A218P9X8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribonuclease P protein component 4</fullName>
            <shortName evidence="8">RNase P component 4</shortName>
            <ecNumber evidence="8">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Rpp21</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rnp4</name>
        <name evidence="9" type="ORF">A3L08_09760</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the eukaryotic/archaeal RNase P protein component 4 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854827.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9X8"/>
    <dbReference type="GeneID" id="33316558"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09760"/>
    <dbReference type="OrthoDB" id="10058at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00757">
        <property type="entry name" value="RNase_P_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016432">
        <property type="entry name" value="RNP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007175">
        <property type="entry name" value="Rpr2/Snm1/Rpp21"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003045">
        <property type="entry name" value="PRK03954.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04032">
        <property type="entry name" value="Rpr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004878">
        <property type="entry name" value="RNase_P_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="7 8" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="124" mass="15223" checksum="9690C9C81A923BDF" modified="2017-09-27" version="1">MSKKKFIRQKEQREKRKVARERVDRLFTLAERVFPHEPELANRYVEIALAVQQKAKIRMPRKWKRRYCKRCHSFLVPGKNAVVRLRSKPYPHVVIKCLSCGHIMRYPYLREHKERRRKERQSPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Y4</accession>
    <name>A0A218P9Y4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein-L-isoaspartate O-methyltransferase</fullName>
            <ecNumber evidence="9">2.1.1.77</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">L-isoaspartyl protein carboxyl methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Protein L-isoaspartyl methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Protein-beta-aspartate methyltransferase</fullName>
            <shortName evidence="9">PIMT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pcm</name>
        <name evidence="10" type="ORF">A3L08_09745</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>[protein]-L-isoaspartate + S-adenosyl-L-methionine = [protein]-L-isoaspartate alpha-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:12705"/>
            <dbReference type="Rhea" id="RHEA-COMP:12143"/>
            <dbReference type="Rhea" id="RHEA-COMP:12144"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90596"/>
            <dbReference type="ChEBI" id="CHEBI:90598"/>
            <dbReference type="EC" id="2.1.1.77"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.77" evidence="9"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854825.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Y4"/>
    <dbReference type="GeneID" id="33316555"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09745"/>
    <dbReference type="OrthoDB" id="33618at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000010">
        <property type="entry name" value="Protein-L-isoaspartate O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00090">
        <property type="entry name" value="PIMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00080">
        <property type="entry name" value="pimt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001453">
        <property type="entry name" value="PRK00312.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579:SF0">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE(D-ASPARTATE) O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01135">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01279">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 9 10" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="9">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ07582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="220" mass="24909" checksum="6DC636677B3F35B5" modified="2017-09-27" version="1">MVDEEELLRRWEKTVEQLFREGVIKSEPVRRAFLKYPRYLFVEERYKKYAHVDEPLPIPAGQTISAPHMVAIMLELADLRPGLNVLEIGTGSGWNAALISELVKTDVYTVERLPELVEFARKNLERAGVKNVHVILGDGSKGFPPKAPYDRIIVTAGAPEVPRPLIQQLNPGGKLIIPVGSYHLWQDLYIIDKTETGKIKKRRWGGVAFVPLVGEYGWNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA06</accession>
    <name>A0A218PA06_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 20">DNA ligase</fullName>
            <ecNumber evidence="18 20">6.5.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19 20">Polydeoxyribonucleotide synthase [ATP/NAD(+)]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">lig</name>
        <name evidence="23" type="ORF">A3L08_01510</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 20">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 20">
            <text>NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide.</text>
            <dbReference type="EC" id="6.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 20 21">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.6" evidence="18 20"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854857.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA06"/>
    <dbReference type="GeneID" id="33314907"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01510"/>
    <dbReference type="OrthoDB" id="31274at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07901">
        <property type="entry name" value="Adenylation_DNA_ligase_Arch_LigB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07972">
        <property type="entry name" value="OBF_DNA_ligase_Arch_LigB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3260.10:FF:000007">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000163">
        <property type="entry name" value="Probable DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.30:FF:000012">
        <property type="entry name" value="Probable DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00407">
        <property type="entry name" value="DNA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022865">
        <property type="entry name" value="DNA_ligae_ATP-dep_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF7">
        <property type="entry name" value="DNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="ATP-dependent DNA ligase DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15 20" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 20" id="KW-0132">Cell division</keyword>
    <keyword evidence="9 20" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13 20" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="14 20" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 20" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 20 23" id="KW-0436">Ligase</keyword>
    <keyword evidence="11 20" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 20" id="KW-0520">NAD</keyword>
    <keyword evidence="8 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="22">
        <location>
            <begin position="327"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="active site" description="N6-AMP-lysine intermediate" evidence="20">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="414"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00052412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00066920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="ASJ07614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="559" mass="63285" checksum="CFC4B15207860F80" modified="2017-09-27" version="1">MRYSELADLYRRLEKTTLKTLKTKFVADFLKKTPDELLEIVPYLILGKVFPDWDERELGVGEKLLIKAVSIATGVPEKEIEDSVKDTGDLGESVALAIKKKKQRSFFSQPLTIKRVYDTFVKVAEAQGEGSQDRKMKYLANLFMDAQPEEGKYIARTVLGTMRTGVAEGILRDAIAEAFKVKPEMVERAYMLTSDFGYVAKVAKLEGNDGLAKVTVQIGKPIRPMLAQNAASVKEALVEMGGKAAFEIKYDGARVQVHKDGDRVVIYSRRLENVTKSIPDVVSAVLESLKAEKVIVEGELVAVGEGGRPRPFQFVLRRFRRKYNIDEMIERIPLELNLFDVLYVDGETLINLPFSERRKRLEETIAESEKIKLAVQLVTNNVDEAQEFYEKALELGHEGVMAKRLDSVYEPGNRGKKWLKVKPTMEDLDLVIIGAEWGEGRRAHLLGSFLVAAFDPHSGEFVPVGKVGSGFTDEDLVEFTKMLKPLIVREEGKFVEIEPKVVIEVTYQEIQKSPKYKSGFALRFPRYVALREDKSPEEADTIERVARLYEFQEKFKAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA29</accession>
    <name>A0A218PA29_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Sodium:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">A3L08_00410</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854844.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA29"/>
    <dbReference type="GeneID" id="33314686"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00410"/>
    <dbReference type="OrthoDB" id="12029at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562">
        <property type="entry name" value="NAPA-TYPE SODIUM/HYDROGEN ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562:SF3">
        <property type="entry name" value="SODIUM ION_PROTON EXCHANGER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="211"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="286"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="321"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="350"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="11">
        <location>
            <begin position="10"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ07600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="382" mass="41871" checksum="9F0F315CB48308BF" modified="2017-09-27" version="1">MEIIGYVFVIIAFARLLAEVFERLGYPGFLGEITAGMILSAVLVGMPRDQLELMAELGLFFLMISAGLEVTPEELHYAGKTTLPLYGITYAVMFATTLPFTGWSAGSDNVISAAILSIASAPIVLRLKRFFGEDFLHVALSYAVISEVMGLFIVYMVVRFHESPGNYAPILWDIFKDAAFIGGILYVNYLIGIRHKVMLIRFLRRLRSDEAVFGLFMILSTSLAFISEEIGMHFTIGGFLAGLLMHSDLLGTKQYDRLTTIISGVTYGIFAPIFFAWRGLNFETELSFVVVEFFVVIYTVRLLLSALAVRRDSLSKSLVRGAGIASFGVLGLLMGEIGFVSGVLSEHMYAMSSLASVLGIFASATVGRLVNYWSPKKASGAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA35</accession>
    <name>A0A218PA35_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Mechanosensitive ion channel protein MscS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_03535</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA35"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03535"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049278">
        <property type="entry name" value="MS_channel_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045275">
        <property type="entry name" value="MscS_archaea/bacteria_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011066">
        <property type="entry name" value="MscS_channel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006686">
        <property type="entry name" value="MscS_channel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221:SF20">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21082">
        <property type="entry name" value="MS_channel_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82689">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01246">
        <property type="entry name" value="UPF0003"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="133"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="8">
        <location>
            <begin position="150"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS C-terminal" evidence="9">
        <location>
            <begin position="223"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ07638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="328" mass="36985" checksum="1C9735509C21951D" modified="2017-09-27" version="1">MLVTGIIIAKVLKKYLLNLSRSTKYVWIINEDTASTLHNLIMVVTLVYTFDALGLLSLKIAGTRISNLLTAFLVFYFSYLLAKKSKEYMIMRSSQQKLPEVQVKAKLFYYTLVTLAFFIALNIAGFTGKLTTLLAAAGITGIVLGFASQTVVSNFISGIFMYFDKPLKIGDPVEVAGYSGIVHDIRILSTRIRTWDGLLVRIPNEKLFNSEIKNLMKYPARRVDVTVGIAYKEDAQKAIEVIKKTLNEMPYVLAEPEPLVFVDNLGDNSVNIAVRAWAPSEKWFDVRTQIVQKIKEALDREGIEIPFPQRVNWFAEELRVKIEGENGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA43</accession>
    <name>A0A218PA43_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Small ribosomal subunit protein uS19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">rps19p</name>
        <name evidence="9" type="ORF">A3L08_04065</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854885.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA43"/>
    <dbReference type="GeneID" id="33315417"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04065"/>
    <dbReference type="OrthoDB" id="30559at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.860.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="entry name" value="30s Ribosomal Protein S19, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_uS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_uS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005732">
        <property type="entry name" value="Ribosomal_uS19_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_uS19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005713">
        <property type="entry name" value="Ribosomal_uS19_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_uS19_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003121">
        <property type="entry name" value="PRK04038.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01050">
        <property type="entry name" value="rpsS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01025">
        <property type="entry name" value="uS19_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="RIBOSOMAL PROTEIN S19P FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="Ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="133" mass="15409" checksum="5647362DAA27B382" modified="2017-09-27" version="1">MARKKEFRYRGYTFEELLNMSLEDFAKLLPARQRRSLKRGLSPEQKKLLRKIRLAKKGKYNKPIRTHSRDMVVLPEMVGMTIHVHNGKEFVPIEIKEEMIGHYLGEFALTRKIVQHGSPGVGATRSSMFVAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA56</accession>
    <name>A0A218PA56_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">A3L08_07020</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="9">Belongs to the Lhr helicase family. Lhr-Core subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854911.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA56"/>
    <dbReference type="GeneID" id="33316007"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07020"/>
    <dbReference type="OrthoDB" id="372104at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17922">
        <property type="entry name" value="DEXHc_LHR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18796">
        <property type="entry name" value="SF2_C_LHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052511">
        <property type="entry name" value="ATP-dep_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017170">
        <property type="entry name" value="Lhr-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013701">
        <property type="entry name" value="Lhr-like_DEAD/DEAH_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045628">
        <property type="entry name" value="Lhr_WH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010338">
        <property type="entry name" value="PRK13767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962">
        <property type="entry name" value="ATP-DEPENDENT HELICASE LHR-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962:SF6">
        <property type="entry name" value="LARGE HELICASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08494">
        <property type="entry name" value="DEAD_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19306">
        <property type="entry name" value="WHD_Lhr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037307">
        <property type="entry name" value="Lhr-like_helic_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="45"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="277"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00093467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ07669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="866" mass="99482" checksum="D01A00E1BE2561FB" modified="2017-09-27" version="1">MSAEGIRRAEREYTDEEIFGILSEPVREWFKRKFGTFTPPQRYAVLEIHKGENVLISSPTGSGKTLSAFLAAINELILLGKEGKLEDRIYVLYVSPLRALNNDIKRNLEGPLMEIQEVAKELGYELPGIRVGIRTSDTSSYQKSKMVKKPPHILITTPESLAIALNAPKFRERLKTIRYLIIDEVHALAENKRGTHLALSVERLAEMAEEKFVRIGLSATIHPLEEVAKFVFGFDDEGKPRPGLIVDVSFAKETEIRVESVVDDLIYTDAGTLSEALYKRLAELIREHRTTLIFTNTRSGAERVAFNLKKRYPEFEGLIEAHHSSLSREVRLDVEERLKRGELKAVVTSTSLELGIDIGTIDLVVLIGSPKSVNRALQRIGRAGHRLHDVSKGIILALDRDDLVEVTVLAHNARNRRLDRVRIPKNPLDVLVQHLLGMALNKVWEVEEAYRVVRRAYPYRDLPFEDFMSVLHYLAGEYAGLEERKVYAKIWLEDGRFGRRGKMTRAIYYMNVGTIPDEAKIRVYTMDKQMIGTVEEEFAERLMPGDIFVLAGRTYEFVKSRGNKIYVIPRQGAKPTIPAWFSEMLPLSFDLALDIQRFRREVSELVNNRRAKDFLMRKYGIDERAARAILAYFREQARYSTVPDDETVLVEVVRGENRNEYFFHTLVGRRANDALSRAFAYLVSQWKRCNVGVAINDNGFALILPPEAELTEDEIRGLFQVEDLRETLKRALDNTELLKRRFRHVANRGLLILRRYIGRSKRLGRQQVMAVALLKVLKENHPDFPLLKEVYREIMEDKMDVENAELFLRWIMEGKVRVVVEEHDVPSPFAFNLEAIGSSDVVLMEDRRELIKQLHRKIMEMIEASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA63</accession>
    <name>A0A218PA63_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Heavy-metal chelation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_08495</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854929.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA63"/>
    <dbReference type="GeneID" id="33316305"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08495"/>
    <dbReference type="OrthoDB" id="40399at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007161">
        <property type="entry name" value="DUF364"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025251">
        <property type="entry name" value="DUF4213"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04016">
        <property type="entry name" value="DUF364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13938">
        <property type="entry name" value="DUF4213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159713">
        <property type="entry name" value="Dhaf3308-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4213" evidence="2">
        <location>
            <begin position="9"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Putative heavy-metal chelation" evidence="1">
        <location>
            <begin position="104"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="245" mass="27206" checksum="8DC23B1E92CF2B23" modified="2017-09-27" version="1">MLLSRIKKEALKLAEGLELVDFGFALPYTWVLAEGPEGKALGVAMTLPEEVQRYRNSISEPSLKAFIERADSLNAIERTLGLAAINAVSQYHIDLSNAEWVDVLELLSEDAGRVALIGNMPPLAKKLRERDFEVFVFERNAKLWDRDTYSDALEYHLLPEMDAVIASASCLVNGTIDMLLERAKRARLFVLTGPTGQLLPEFLKGTGVTHLAAMKVVDIEKALLGLKLGSFRGFEEGNRKYVVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA64</accession>
    <name>A0A218PA64_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Putative HTH-type transcriptional regulatory protein A3L08_07635</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_07635</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854919.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA64"/>
    <dbReference type="GeneID" id="33316130"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07635"/>
    <dbReference type="OrthoDB" id="31424at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00584">
        <property type="entry name" value="HTH_type_cro_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020886">
        <property type="entry name" value="MTH_967-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059051">
        <property type="entry name" value="MTH_967_PDDEXK"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003162">
        <property type="entry name" value="PRK04140.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26553">
        <property type="entry name" value="PDDEXK_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="5">
        <location>
            <begin position="131"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="318" mass="35891" checksum="5188AC8FE3EAD9D6" modified="2017-09-27" version="1">MDRERLIKTVEAVLRGAGYKTARMDFRGSCFDVVASRLMVLLFIKVATNIDTVTEEQAEDLKRLASFFNASPLIVGVKTKNAELEEGVVYERFGIYAVRPETLYDILAENELPAIFAERGGFYVKVNGALLKRLREERGYSINELAQLLGVSRKSLINYERGEQAVSLEVAIRMEELFDEPLAEPIDVLSARVEANLNVNPETPLEKEIFDRLKKLGLGVVKVKKAPFNAVSKEDEFRILTGIDERKTRSTVKRAEMVAEVGKIINSDGVFILERTRKEVVGDVPLIPKDSLEEVKDADELIDLIEELKKEIKRQLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA65</accession>
    <name>A0A218PA65_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_07440</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclic nucleotide phosphodiesterase class-III family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA65"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07440"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07400">
        <property type="entry name" value="MPP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050884">
        <property type="entry name" value="CNP_phosphodiesterase-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42988:SF2">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE CBUA0032-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42988">
        <property type="entry name" value="PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="5">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="5">
        <location>
            <begin position="272"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="498" mass="56924" checksum="3881AAFC88257FC5" modified="2017-09-27" version="1">MRIAHISDTHITSEGAFKGYAFDLIVEEINRGNFDFVIHTGDITNQGLREEYEQAAYQLKKIKKPLVVLPGNHDVRNVGYKLFEKFIGPLNGVYEFKDGVVIWVDSTIPDLSDGRIGGHKFRWLKEKLEEYSGKKFKIVAAHHHLVPLPDTGRERNVLFNAGDVLDLLLRNEVNLYTCGHKHVPNVYRVEDLVIDNAGCTSCKKTRRGDVNSYSIIELRDDGSVKVTIRRVTGDETSKEHRPVRPKIFVPSGKRVMRIVQMSESNVSDRVYFRKKTLENVIRMINERLKPDLVIHNGDIVDAGIERYYERAYGYYREVKVPKIVIPGHNDITYLGYELFQEYFGEPEITELNDVVIIPLLSAQYETPIGVVGRMGQRRLRELLQEYAGKFRIVALHHNVIPIHRSREIGFLEDAGDVLKVLTDEGTELVLTGHGGNAYAVKIERTPIVNSGSVSWELHRNPFGNSFNIVDIYTDMVVVWEVQSTWGSRKLLGIWKRKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA68</accession>
    <name>A0A218PA68_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_05085</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in DNA damage repair.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Nre family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854897.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA68"/>
    <dbReference type="GeneID" id="33315621"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05085"/>
    <dbReference type="OrthoDB" id="6609at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02096">
        <property type="entry name" value="Nre"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033167">
        <property type="entry name" value="Nre"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006979">
        <property type="entry name" value="Nre_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006978">
        <property type="entry name" value="Nre_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38136">
        <property type="entry name" value="DNA REPAIR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38136:SF2">
        <property type="entry name" value="DNA REPAIR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04895">
        <property type="entry name" value="Nre_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04894">
        <property type="entry name" value="Nre_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Archaeal Nre N-terminal" evidence="2">
        <location>
            <begin position="20"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Archaeal Nre C-terminal" evidence="3">
        <location>
            <begin position="297"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="422" mass="48677" checksum="F6B8F8FC75A9D206" modified="2017-09-27" version="1">MSAPVFNSKLCAVCKGRKLLCGRPTCPILERFRVARSVEQKLNKRHLFGSSPPSIFVGEYGYPKVRIGPLVPPIEGNTDYLDNPLKWENKTIKDILYYRSLLVMGESEADVNVRKSGRILGEVQELAMSIKPVDSEILLKRKPVLKVLPSEFAPPVGPKAELLDFELTENPKIPRRTDYVVSDELKAEQAIMRLYNWGFDEYYIIRLLSAGLLGVDKKLVPTRWSITAVQDTIGKNLRREVLHYPEINDYEVYFYRFLGNRYAVLLMPETYAFELLEVWLKGSLFGTSEPNVIHDYEDFRGRKEYVKETAGAYHAARLSVLEALRGRRRQARAVVFREVTPEYYAPVGVWQIRLGVKKAMGNPIGRFETLNEALEAIKRRLEHPFEKYLERSYILRNLARQKTLDEWLGKNIYRLDRENAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA70</accession>
    <name>A0A218PA70_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA 5'-3' helicase</fullName>
            <ecNumber evidence="15">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">A3L08_09230</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. RAD3/XPD subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="15"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854942.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA70"/>
    <dbReference type="GeneID" id="33316452"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09230"/>
    <dbReference type="OrthoDB" id="27512at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.20">
        <property type="entry name" value="Bacterial XPD DNA helicase, FeS cluster domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010643">
        <property type="entry name" value="HBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006554">
        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013020">
        <property type="entry name" value="Rad3/Chl1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042493">
        <property type="entry name" value="XPD_DNA_FeS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00604">
        <property type="entry name" value="rad3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF34">
        <property type="entry name" value="REGULATOR OF TELOMERE ELONGATION HELICASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06733">
        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06777">
        <property type="entry name" value="HBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 19" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="6"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="28"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ASJ07696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="643" mass="73024" checksum="1D93DA652FB34D1D" modified="2017-09-27" version="1">MSEPSDAFEYFPYRGLRPNQREFIELVSEAVRNGENAIIEAPTGFGKTVSVLAGILPYAKEEGYKVLYLARTHRQMDRVVEELKAISGKNPVSGVELRSRKELCLHNYLTQFTTDAYTAMVVCKNLKKLGKCPFYENEKKKKAEFDELVRFFLESPSHPAEILSYAETLELCPYDLTKRMAEKADVIVASYLYLLSPSIRENFISSLDMDYSDLIVVFDEAHNLPDQAISALSDKISINTVNRAIKEADEYNEHEIANFLSIFGRGLEILFQEKLGSKDVQETPIQPELVFEHVVSVLNFDTRWLVKTLNDMVAVGDSIREDRIEKGKPPRSYIGRVGEFLLLWLSLIGREDYLFILSRDRGLSLELVALDPSKALGFIRDVQSAIFMSGTLTPLEAFRDIMGIENARLKKFPRMVKSENAQVLVAKDVSTRGEERSLQVYRKMVDYIVEAAKIIPKNVGVFTASYEVLQGLLSANLQVRLEETGKAVFVEKQGVSSAENDSLVRAFKAHAKGNGAVLLGVMGGRNSEGQDYSGDEMNGVILVGIPYARPTPRVQAQIRYFERKFPEKGRYYGYFLPAHRKLVQAAGRVHRSAEEKGSIIVLDYRLLWRGIRKDLPDWMVETMRPVDLGRMRLYLRRFWTNGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA71</accession>
    <name>A0A218PA71_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dinitrogenase iron-molybdenum cofactor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854930.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA71"/>
    <dbReference type="GeneID" id="33316308"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08510"/>
    <dbReference type="OrthoDB" id="25911at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00851">
        <property type="entry name" value="MTH1175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.130">
        <property type="entry name" value="Dinitrogenase iron-molybdenum cofactor biosynthesis domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003731">
        <property type="entry name" value="Di-Nase_FeMo-co_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036105">
        <property type="entry name" value="DiNase_FeMo-co_biosyn_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033913">
        <property type="entry name" value="MTH1175_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051840">
        <property type="entry name" value="NifX/NifY_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33937:SF2">
        <property type="entry name" value="DINITROGENASE IRON-MOLYBDENUM COFACTOR BIOSYNTHESIS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33937">
        <property type="entry name" value="IRON-MOLYBDENUM PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02579">
        <property type="entry name" value="Nitro_FeMo-Co"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53146">
        <property type="entry name" value="Nitrogenase accessory factor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dinitrogenase iron-molybdenum cofactor biosynthesis" evidence="1">
        <location>
            <begin position="13"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="203" mass="20915" checksum="0E6F581353FA55C8" modified="2017-09-27" version="1">MRIAIPTNGGGLNDTVAPVFARAPAFYIADVDENGNVISEKVIQNGAAMAGGGAGPMAVQTLINEGVEAIVAPQVGPNALGAIQAAGIRLYQVAPGTPVEEAIKAVTSGSVGQFTAPTPAAPVVPSTPTTPAPAYGPYPTAPAYPPYPAYGYGPRGRGRGGRGRGWGRGWGRGGRGWGARLGYCPWTGQPSRRAWLARFFGWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PAD4</accession>
    <name>A0A218PAD4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Molybdate/tungstate-binding protein WtpA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_09800</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial solute-binding protein 1 family. WtpA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PAD4"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09800"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030973">
        <property type="term" value="F:molybdate ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901359">
        <property type="term" value="F:tungstate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015689">
        <property type="term" value="P:molybdate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13540">
        <property type="entry name" value="PBP2_ModA_WtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000440">
        <property type="entry name" value="Uncharacterized solute-binding protein MA_0280"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022498">
        <property type="entry name" value="ABC_trnspt_W-bd_WtpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050682">
        <property type="entry name" value="ModA/WtpA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003196">
        <property type="entry name" value="PRK04168.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03730">
        <property type="entry name" value="tungstate_WtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30632">
        <property type="entry name" value="MOLYBDATE-BINDING PERIPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30632:SF16">
        <property type="entry name" value="MOLYBDATE_TUNGSTATE-BINDING PROTEIN WTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13531">
        <property type="entry name" value="SBP_bac_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00074198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="335" mass="37113" checksum="A3B5707A118961B8" modified="2017-09-27" version="1">MAVAVLAAGCIGGSNGSSGSNLEEKTLIVFHAGSLSVPFQQLEKEFSDYAEKNLGVKVTFQDEASGSVKAVRKVTDLGKKADIVAVADYTLIPQLMVPNYTDFYVLFATNEIVIAFTNESKYADEIDSDNWYEILARPGVTFGFSDPNQDPCGYRSVMVMKLADLYYGKPAFETLVEKNTNIYANGTHIYVPKEIQLKTNKVVIRPKETDLTGLVESGSLDYFFIYKSVAEQHNLRYITLPDEINLRDFGRADFYGQVSITLGSTGKTIKAKPIVYGVTVPKDAPNRELAIEFLKFLLGENGQRVFRENHQDFLNPPVAFGNVPEEIKALVKVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P4Y4</accession>
    <name>A0A218P4Y4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Transcription initiation factor IIB</fullName>
            <shortName evidence="10">TFIIB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 13" type="primary">tfb</name>
        <name evidence="13" type="ORF">A3L08_00095</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853107.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P4Y4"/>
    <dbReference type="GeneID" id="33314623"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00095"/>
    <dbReference type="OrthoDB" id="7429at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20549">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20550">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000023">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.170:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00383">
        <property type="entry name" value="TF2B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023484">
        <property type="entry name" value="TFIIB_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001658">
        <property type="entry name" value="PRK00423.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="12">
        <location>
            <begin position="3"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="repeat" description="1" evidence="10">
        <location>
            <begin position="120"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="repeat" description="2" evidence="10">
        <location>
            <begin position="214"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00053882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ05844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="303" mass="34239" checksum="6009CBE912085B5F" modified="2017-09-27" version="1">MADKRVCPVCGSTEFIYDPRRGEIVCAKCGYVLEENVIDEGPEWRAFDPDQRAKRARTGAPMTLMIHDKGLSTDIDWRDKDIHGNQISGMYRNKLRRLRMWQRRMRINDAAERNLAFALSELDRMAAQMRLPRRVKEAAASLYRKAVMKKLIRGRSIEGMVSAALYAACRMEGIPRTLDEIAAVSKVSKKEIGRSYRFMARGLNLNLRPTSPIEYVDRFGDALNVSARTKKRAKEILREAIEKGITSGKGPTGLAAAALYVASLLEGEKKTQREVAEVAHVTEVTVRNRYKELVEKLGINVPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P4Z1</accession>
    <name>A0A218P4Z1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">MazF family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_00020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853092.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P4Z1"/>
    <dbReference type="GeneID" id="33314607"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00020"/>
    <dbReference type="OrthoDB" id="94628at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003477">
        <property type="entry name" value="PemK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011067">
        <property type="entry name" value="Plasmid_toxin/cell-grow_inhib"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33988">
        <property type="entry name" value="ENDORIBONUCLEASE MAZF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02452">
        <property type="entry name" value="PemK_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50118">
        <property type="entry name" value="Cell growth inhibitor/plasmid maintenance toxic component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ05830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="119" mass="13482" checksum="6E35C2E45EC1F55B" modified="2017-09-27" version="1">MPRSSSNFRQGEILLLELPFTDYLGKKLRPVLVVSSDELNKVSDDLIVLKITGSPHFEEFPVELEQKDLLRGKLKKKSFIDCSSVFTVEKSLIIKSIGEIGPEKMDEVKEILKRTFSIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P504</accession>
    <name>A0A218P504_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_00195</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GDT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853127.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P504"/>
    <dbReference type="GeneID" id="33314643"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00195"/>
    <dbReference type="OrthoDB" id="85362at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001727">
        <property type="entry name" value="GDT1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12608:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 165"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12608">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN HTP-1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01169">
        <property type="entry name" value="GDT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ05864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="87" mass="9063" checksum="A9E86E9C830C24D5" modified="2017-09-27" version="1">MDGILAIFFAIFLAELGDKTQLATIAFASKYGWKTAFTGAVLGLAAVNLLGAILGDKLGDVLPIDLVHKGAGVLFILFGVLMLLGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P559</accession>
    <name>A0A218P559_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GNAT family N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853185.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P559"/>
    <dbReference type="GeneID" id="33314706"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00510"/>
    <dbReference type="OrthoDB" id="38613at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051556">
        <property type="entry name" value="N-term/lysine_N-AcTrnsfr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42919:SF35">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42919">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="7"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="167" mass="19572" checksum="A6DFB2D99F25A1F1" modified="2017-09-27" version="1">MTEVKVEKLKEFNQEILERLIEIYMRGYEGMREYGGEGESYAKRYLRWCWGKAKDGFFVAKVGDEIAGFIVCDNDWYSKYEGRTVGAIHEFVVDKKFQGHGIGHKLMEKCLDYLSKYNNRIELWVGEKNEGAMRFYEGYGFRKVGQSGIWVRMVKDVRKDDKGSGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P585</accession>
    <name>A0A218P585_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00590</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853200.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P585"/>
    <dbReference type="GeneID" id="33314722"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00590"/>
    <dbReference type="OrthoDB" id="97196at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="75" mass="8819" checksum="0F302278D8C87BC8" modified="2017-09-27" version="1">MRWFGVLLVFIGLLVLLKQFEPAFLEPLKSYAPYIKDAFWGVTLIAFGFYIMLRKTARRVVLAIYLIYLLLYLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5B3</accession>
    <name>A0A218P5B3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">XRE family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00775</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853231.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5B3"/>
    <dbReference type="GeneID" id="33314759"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00775"/>
    <dbReference type="OrthoDB" id="371772at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017271">
        <property type="entry name" value="Tscrpt_reg_HTH_MJ1545_prd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037724">
        <property type="entry name" value="TF_HTH_MJ1545_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="27"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="243" mass="27015" checksum="0470E5819B4B2C4F" modified="2017-09-27" version="1">MLEKEKEALAKRIAGEITLSSDPGKTMRKWREIFGISQTELAEYLGVSSSVISDYEGGRRKSPGASTIRKFVEALLDIDEKRGGNVIRAFSKTLGSELPTSAILDIREFALPVTVKDLVDAVSGEVVANIHLIDRRIYGYTVVDSIRAILEMSSEEFLKLYGWTTERALVFTKVTTGRSPMIAVRVQGLKPAVVVLHGVKKLDELAVKLAERERVPLVVSKLPSESDLITNLRKLVEKTEREF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5C4</accession>
    <name>A0A218P5C4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853258.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5C4"/>
    <dbReference type="GeneID" id="33314792"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00940"/>
    <dbReference type="OrthoDB" id="28313at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050114">
        <property type="entry name" value="UPF0173_UPF0282_UlaG_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43546:SF8">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43546">
        <property type="entry name" value="UPF0173 METAL-DEPENDENT HYDROLASE MJ1163-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13483">
        <property type="entry name" value="Lactamase_B_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="7"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="211" mass="24294" checksum="967D93021683D21A" modified="2017-09-27" version="1">MRIIWYGHSCFWIETKGVRLLIDPYPEVDDDRIGEVDYILLTHEHVDHYGKVELLSRLRDATVIGPPTVYMMAISDGVTKAREIREGQTIELENGVKVTAFYMEHPSSQYPLGYLIEGDKRLFHAGDTYSTPNLQKLRGKVDVLLVPISGRSTANEREAAQIVEDIRPRVVIPMHYGVYSDGDPEKLREELQKRRIWALVKPLELYEELTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5D7</accession>
    <name>A0A218P5D7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_00790</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5D7"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00790"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ05971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="77" mass="8957" checksum="183D4B85186CA5F2" modified="2017-09-27" version="1">MESFSNRLSNRQFKKNHELLVKLLPKASALTERLRAIMEMLISKGIYSLITISRVFALPKGVWRMLELIANLFKGFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5F4</accession>
    <name>A0A218P5F4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ribosomal-protein-alanine N-acetyltransferase RimI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_00995</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acetyltransferase family. RimI subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853269.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5F4"/>
    <dbReference type="GeneID" id="33314803"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00995"/>
    <dbReference type="OrthoDB" id="43754at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006464">
        <property type="entry name" value="AcTrfase_RimI/Ard1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050680">
        <property type="entry name" value="YpeA/RimI_acetyltransf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01575">
        <property type="entry name" value="rimI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43420">
        <property type="entry name" value="ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43420:SF12">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="5">
        <location>
            <begin position="17"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="167" mass="18946" checksum="2C97CCDD1F27C5EA" modified="2017-09-27" version="1">MSVSIRRAGRRIPLAMVSIRPAKLFDIPEVVRIERASFNEEYPRGVFLAFLENNPSTFLVAEYGGRVIGYVMGYLRPDLEGHIMSIAVDPEYRGNGIGSALLTEVIERLINEGARYIGLEVRVSNETAIRLYESFGFRKIKRIIGYYSDGEDAYYMLLPAEEWGGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5I3</accession>
    <name>A0A218P5I3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Methylated-DNA--protein-cysteine methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.63</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">A3L08_01265</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:53428"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:13555"/>
            <dbReference type="Rhea" id="RHEA-COMP:13556"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:137386"/>
            <dbReference type="ChEBI" id="CHEBI:137387"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] = S-methyl-L-cysteinyl-[protein] + a 2'-deoxyguanosine in DNA</text>
            <dbReference type="Rhea" id="RHEA:24000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:11367"/>
            <dbReference type="Rhea" id="RHEA-COMP:11368"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:85445"/>
            <dbReference type="ChEBI" id="CHEBI:85448"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MGMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.63" evidence="5"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853316.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5I3"/>
    <dbReference type="GeneID" id="33314857"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01265"/>
    <dbReference type="OrthoDB" id="372118at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003908">
        <property type="term" value="F:methylated-DNA-[protein]-cysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06445">
        <property type="entry name" value="ATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000214">
        <property type="entry name" value="Methylated-DNA--protein-cysteine methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.70">
        <property type="entry name" value="Methylated DNA-protein cysteine methyltransferase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054936">
        <property type="entry name" value="DNA_protcyst_Mta_Thcoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001497">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015236">
        <property type="entry name" value="MGMT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036631">
        <property type="entry name" value="MGMT_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041132">
        <property type="entry name" value="DNA_protcyst_Mta_Thcoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00589">
        <property type="entry name" value="ogt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003022">
        <property type="entry name" value="PRK03887.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46460">
        <property type="entry name" value="METHYLATED-DNA--PROTEIN-CYSTEINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46460:SF1">
        <property type="entry name" value="METHYLATED-DNA--PROTEIN-CYSTEINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09153">
        <property type="entry name" value="MGMT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53155">
        <property type="entry name" value="Methylated DNA-protein cysteine methyltransferase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="Methylated DNA-protein cysteine methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00374">
        <property type="entry name" value="MGMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methylated DNA-protein cysteine methyltransferase ribonuclease H-like" evidence="14">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Methylated-DNA-[protein]-cysteine S-methyltransferase DNA binding" evidence="13">
        <location>
            <begin position="91"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF09153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ASJ06054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="179" mass="20235" checksum="1223B57F88A97795" modified="2017-09-27" version="1">MLSAEVFEIAGRRVWIGVFWQEKIQGITFSLDREQFERNVERLSGFLRRRGVDLDLAREGSNYPALVRDVIIGKVDNADVLDELSFEGLTPFEGRVYEWLTKNVKRGDIITYGNLAKALGTSPRAIGGAMRRNPYPIVVPCHRVVAKDGIGYYTPRLDEKVFLLKIEGVEGWTSSKLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5J5</accession>
    <name>A0A218P5J5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Regulator of amino acid metabolism, contains ACT domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01385</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853339.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5J5"/>
    <dbReference type="GeneID" id="33314882"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01385"/>
    <dbReference type="OrthoDB" id="30884at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014424">
        <property type="entry name" value="UCP004897_ACT"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004897">
        <property type="entry name" value="UCP004897_ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="196" mass="22935" checksum="400242577409F225" modified="2017-09-27" version="1">MMLILEAYFKNYPARRKVAEFLFENGLSVKNGKVYLRDVEVPISELARIIGVNRKIVYHTIEYIEKTYPLKLIFAKLNPLPSLIDVAPLMGWEVLEIELDKDRYLQGFSDVLRLLYENGVPVMEVFSRNLREEPTKLYIVIDGTLPVDVFVRVKEMEGFRKLILHTPEKDKEKFVCNYCEVKYCPKRVLVEKMASP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5K0</accession>
    <name>A0A218P5K0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pyruvate/ketoisovalerate oxidoreductases common subunit gamma</fullName>
            <ecNumber evidence="3">1.2.7.1</ecNumber>
            <ecNumber evidence="2">1.2.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_01305</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2 oxidized [2Fe-2S]-[ferredoxin] + pyruvate + CoA = 2 reduced [2Fe-2S]-[ferredoxin] + acetyl-CoA + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12765"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="1.2.7.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3-methyl-2-oxobutanoate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = 2-methylpropanoyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11712"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57338"/>
            <dbReference type="EC" id="1.2.7.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of one alpha, one beta, one delta and one gamma chain.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.1" evidence="3"/>
    <dbReference type="EC" id="1.2.7.7" evidence="2"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853325.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5K0"/>
    <dbReference type="GeneID" id="33314866"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01305"/>
    <dbReference type="OrthoDB" id="372091at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043807">
        <property type="term" value="F:3-methyl-2-oxobutanoate dehydrogenase (ferredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051626">
        <property type="entry name" value="Oxidoreductase_gamma_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011894">
        <property type="entry name" value="PorC_KorC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02175">
        <property type="entry name" value="PorC_KorC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006321">
        <property type="entry name" value="PRK08534.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010632">
        <property type="entry name" value="PRK14029.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43366">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43366:SF1">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate/ketoisovalerate oxidoreductase catalytic" evidence="8">
        <location>
            <begin position="10"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="185" mass="19889" checksum="D10623721AB4915F" modified="2017-09-27" version="1">MIEIRFHGRGGQGAVTAANILASAAFKSGKYVQAFPFFGVERRGAPVTAFTRIDEKPIRIKTQIYEPDIVVVLDPSLLDTVDVTAGLKDNGIVIVNTEKSKEEVLEKLKKKPAKLALVDATTIALEILGLPITNTAILGAVAKATGIVELDHVKEAIQETFSGALGEKNAKAAEESFNRTVIYEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5K3</accession>
    <name>A0A218P5K3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">GlcNAc transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_01270</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853317.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5K3"/>
    <dbReference type="GeneID" id="33314858"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01270"/>
    <dbReference type="OrthoDB" id="50301at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13174">
        <property type="entry name" value="TPR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="217"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="223"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="305"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="342" mass="39689" checksum="5420CE376E1B27C3" modified="2017-09-27" version="1">MDKLKAYLIGFLIAVIAIAAGIVWYGGWKLLLQVILTLGFLGVTLMLLFFTGLTLYAESWKYGAILAIFTAISGYGLYLSATWQKLNVVAGIIVFFIAVVAFGIWYISEPDLGLVDRFKSAESLERAGKYKQAARKYEKAGNYLKAAEMYLKLGWMESAAWAYEKAGKYEKAAEIYEGLYEKEKDTYYLKEAHEYWKKAGNMERAAKALERYAEEEPWFWEDVAKLYEELGNEEKAKEAWEKALEYYKKEAEEEGVFWEDVGNIARKLGMEELAREAYQKFLEYCLKEAEEDPMWWKHVAEAYEYLGEKEKAEEARKKYEEYRQEIMKANEETSNFPGEKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5L7</accession>
    <name>A0A218P5L7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pyruvate synthase subunit PorA</fullName>
            <ecNumber evidence="2">1.2.7.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Pyruvate oxidoreductase alpha chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Pyruvic-ferredoxin oxidoreductase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">porA</name>
        <name evidence="10" type="ORF">A3L08_01330</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2 oxidized [2Fe-2S]-[ferredoxin] + pyruvate + CoA = 2 reduced [2Fe-2S]-[ferredoxin] + acetyl-CoA + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12765"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="1.2.7.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of one alpha, one beta, one delta and one gamma chain.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.1" evidence="2"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853330.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5L7"/>
    <dbReference type="GeneID" id="33314871"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01330"/>
    <dbReference type="OrthoDB" id="372068at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000010">
        <property type="entry name" value="Pyruvate ferredoxin oxidoreductase, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000012">
        <property type="entry name" value="Pyruvate:ferredoxin (Flavodoxin) oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050722">
        <property type="entry name" value="Pyruvate:ferred/Flavod_OxRd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053390">
        <property type="entry name" value="Pyruvate_synthase_PorA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040682">
        <property type="entry name" value="PorA_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006233">
        <property type="entry name" value="PRK08367.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154">
        <property type="entry name" value="PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154:SF0">
        <property type="entry name" value="PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate flavodoxin/ferredoxin oxidoreductase pyrimidine binding" evidence="8">
        <location>
            <begin position="16"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate:ferredoxin oxidoreductase core" evidence="9">
        <location>
            <begin position="262"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="394" mass="43854" checksum="617E1BE9B1805DEE" modified="2017-09-27" version="1">MPIRTVMKANEAAAWAAKLAKPKVIAAFPITPSTLVPEKISEFVADGELDAEFIKVESEHSAISACVGASAAGVRTFTATASQGLALMHEILFIAAGMRLPIVIAVGNRALSAPINIWNDWQDTISERDTGWLQFYAENNQEALDLILMAFKVAEDERVLLPAMVGFDAFILTHTVEPVEIPDQEVVDEFLGEYEPKHAYLDPARPITQGTLAFPAHYMEARYTVWEANENAKKVIDEVFAEFEKKFGRKYQKIEEYRTEDAEIIFVTMGSLAGTVKEYVDHLREKGIKAGAAKLTVYRPFPTEEVRELAKKAKVLALLEKNVTFSVGGALFQDFSRALVNQKEKPILVDFILGLGGRDVTFKELDEVLEISKKALAGEEFDEVNWIGLRKEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5M7</accession>
    <name>A0A218P5M7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Iron-sulfur cluster carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_01340</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">Binds and transfers iron-sulfur (Fe-S) clusters to target apoproteins. Can hydrolyze ATP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853332.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5M7"/>
    <dbReference type="GeneID" id="33314873"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01340"/>
    <dbReference type="OrthoDB" id="8297at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001119">
        <property type="entry name" value="Iron-sulfur cluster carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044304">
        <property type="entry name" value="NUBPL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961">
        <property type="entry name" value="IRON-SULFUR PROTEIN NUBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961:SF2">
        <property type="entry name" value="IRON-SULFUR PROTEIN NUBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00074706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="297" mass="32184" checksum="C02862743CBB04BC" modified="2017-09-27" version="1">MTIKAPTLNIGGLGADPLTQRISEKQTKWKYKIAVLSGKGGVGKSTVAVNLATALAKKGYFVGILDADIHGPNVAKMLGVDRADVLAEKLEDGRFEMIPPMNDFMGQTTPIKIMSMGFLVPEDQPIIWRGSLVTKAIKQLLGDVKWGELDFMIIDFPPGTGDEILTVTQSLKLDAAVIVTTPQEVALLDTGKAVNMMKKMEVPYIAVVENMSYLICPHCGNEIDLFGKGGGRKLAEKEGVDFLGEIPIDLKAREASDAGIPIVLYEDTVAAKAFMEIIDKLVKKLEEMKDDTGSESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Q3</accession>
    <name>A0A218P5Q3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cob(I)yrinic acid a,c-diamide adenosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01585</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853374.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5Q3"/>
    <dbReference type="GeneID" id="33314922"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01585"/>
    <dbReference type="OrthoDB" id="24392at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008817">
        <property type="term" value="F:corrinoid adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00561">
        <property type="entry name" value="CobA_ACA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003724">
        <property type="entry name" value="CblAdoTrfase_CobA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00708">
        <property type="entry name" value="cobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004637">
        <property type="entry name" value="PRK05986.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46638">
        <property type="entry name" value="CORRINOID ADENOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46638:SF1">
        <property type="entry name" value="CORRINOID ADENOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02572">
        <property type="entry name" value="CobA_CobO_BtuR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015617">
        <property type="entry name" value="Adensltrnsf_CobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="175" mass="19502" checksum="D50F30716600357B" modified="2017-09-27" version="1">MSWKEKLGLVHIYTGNGKGKTTAALGLAVRMLGSGGRVIIIQFMKAPDVYGEQKKIRECGAVIESFGLPKFVHGKPEPDDIEAAKRALERAREVVSSGEWDLVILDEICVALGFKMLNVEEVKGLIESKAPHTELVLTGRYCPEELFELADYVTEMKEIKHPYQRGILARRGVEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5T0</accession>
    <name>A0A218P5T0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01695</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5T0"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01695"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="66" mass="7887" checksum="E9B035A7BDFE36FE" modified="2017-09-27" version="1">MEGYNTLKPELYNFEVDGKFFVLHPPTGTLLEVDKKTYDFCRFVAELGWEDARERFVTELTFCWGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5W2</accession>
    <name>A0A218P5W2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 7 8">Triosephosphate isomerase</fullName>
            <shortName evidence="7">TIM</shortName>
            <shortName evidence="7">TPI</shortName>
            <ecNumber evidence="7 8">5.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Triose-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tpiA</name>
        <name evidence="9" type="ORF">A3L08_01995</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7 8">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7 8">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="7 8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853449.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5W2"/>
    <dbReference type="GeneID" id="33315004"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01995"/>
    <dbReference type="OrthoDB" id="9465at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00189"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046166">
        <property type="term" value="P:glyceraldehyde-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000223">
        <property type="entry name" value="Triosephosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_A">
        <property type="entry name" value="TIM_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022891">
        <property type="entry name" value="Triosephosphate_isomerase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020861">
        <property type="entry name" value="Triosephosphate_isomerase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003302">
        <property type="entry name" value="PRK04302.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139:SF42">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="Triosephosphate isomerase (TIM)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00171">
        <property type="entry name" value="TIM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7 8" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="4 7 8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 7 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Electrophile" evidence="7">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="12"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="205"/>
            <end position="206"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00019397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="226" mass="23831" checksum="AFD7743F700A6232" modified="2017-09-27" version="1">MEKLKEPIIAINFKTYIEATGERALAIAKAAEKVWKETGITIVVAPQLADLRMIAESVEIPVFAQHIDPIKPGSHTGHVLPEAVKEAGAIGTLLNHSERRMVLADLEASIRRAEEVGLMTMVCSNNPAVSAAVAALGPDYVAVEPPELIGTGIPVSKAKPEVITDTVELVRKANPEVKVLTGAGISTGEDVKKALELGSVGVLLASGVTKAKDPEKAIRDLVSLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Z3</accession>
    <name>A0A218P5Z3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Peptide ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_02150</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853477.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5Z3"/>
    <dbReference type="GeneID" id="33315035"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02150"/>
    <dbReference type="OrthoDB" id="44105at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30465">
        <property type="entry name" value="INNER MEMBRANE ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30465:SF0">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN APPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="197"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="113"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="343" mass="38709" checksum="12123447AD8075C1" modified="2017-09-27" version="1">MRLPIRTLTRLVAVYLGVLLVIVLVAGASSNKVAWEYTHEAVRHIRNSNPAFYEELERNATREGLTVEEYYYRAITKAKGVRTDNLLLMGIDLLRKSFDYYRQDPKYDFVHVIEVSLAVMGLAIVLTILLGIFLGFKLTNGRFLGTAEGLARFFNGLPSWWIGAVLIAVFAVELELFPIAGLRSTPPKEGFDDLLDVLWHLVLPVSTLVFVYVWEFIVTVAHEVRNELGKPYVLTERAKGLPEGIIYRKHVLRNVSIVLSSFTVQKFVEMFTDYIVIDVLFGLGGLGTLLRASFVRTIVPAIGVVVRFDYSLFFVVTLTITTITFLFSLLLELIKGILDPRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P620</accession>
    <name>A0A218P620_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">UDP-glucose 6-dehydrogenase</fullName>
            <ecNumber evidence="3 7">1.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">A3L08_02310</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>UDP-alpha-D-glucose + 2 NAD(+) + H2O = UDP-alpha-D-glucuronate + 2 NADH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:23596"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="1.1.1.22"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.22" evidence="3 7"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853506.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P620"/>
    <dbReference type="GeneID" id="33315067"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02310"/>
    <dbReference type="OrthoDB" id="59839at2157"/>
    <dbReference type="UniPathway" id="UPA00038">
        <property type="reaction ID" value="UER00491"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003979">
        <property type="term" value="F:UDP-glucose 6-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006065">
        <property type="term" value="P:UDP-glucuronate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.100">
        <property type="entry name" value="Cytochrome c1, transmembrane anchor, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028357">
        <property type="entry name" value="UDPglc_DH_bac"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03026">
        <property type="entry name" value="NDP-sugDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43750">
        <property type="entry name" value="UDP-GLUCOSE 6-DEHYDROGENASE TUAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43750:SF3">
        <property type="entry name" value="UDP-GLUCOSE 6-DEHYDROGENASE TUAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500134">
        <property type="entry name" value="UDPglc_DH_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000124">
        <property type="entry name" value="UDPglc_GDPman_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52413">
        <property type="entry name" value="UDP-glucose/GDP-mannose dehydrogenase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 10" id="KW-0520">NAD</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDP-glucose/GDP-mannose dehydrogenase C-terminal" evidence="11">
        <location>
            <begin position="317"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="156"/>
            <end position="159"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="253"/>
            <end position="257"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="427" mass="47822" checksum="FA36921FD786A267" modified="2017-09-27" version="1">MRISVIGSGYVGLVTGMGFVKLGNEVIFVDVDENKIQMINNAQPPIYEEGLEELMREFKGKYHATKNYREAILNSDVTFIAVGTPSREDGSIDLKYVKAASKSIGEALREKTGHHVIVVKSTVLPGTTEEVVKPILEETSGKKAFKDFGLAMNPEFLREGVALKDFLNPDRIVIGTQDERTRKVLEELYAPINAPKLFTDIKTAEMIKYASNAFLATKISFANEIGNICKKLGIDSWKVFEGVGLDHRISPYFFRTGIGWGGSCFPKDTRALIRKAEELGEDPIIIKAAVEVNERQPLKLIELLKKHVPDLKGRKVGLLGLAFKPNTDDVRETRAYIVVKKLLEEGANVIAYDPQGMGNFKRFYPDVGERIKYASTPEEVLNSTDVILIVTEWPQFEELDYSGRIVIDGRRVRAAERTARVYEGVCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P628</accession>
    <name>A0A218P628_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cell division protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P628"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02140"/>
    <dbReference type="OrthoDB" id="93249at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="170" mass="18368" checksum="F683336ED23CF029" modified="2017-09-27" version="1">MKKLAGNVLLTAGLIAGSIAAARIPPMWGGLAASLAVMGAGIVLRRQGAREELHRAAESGTGGVGELERLLGEAIGRLEKILDAPAEKAHAELTKILEELDEFAEKAQPLRIEGLMTYGKIMSIFSRGERALNRAWSAFADGYEKEGRKYLRYGYEDLKETLAAVRAMKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P629</accession>
    <name>A0A218P629_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphatidate cytidylyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853490.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P629"/>
    <dbReference type="GeneID" id="33315050"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02225"/>
    <dbReference type="OrthoDB" id="103686at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="140" mass="15783" checksum="ADD5A2BBCDB177E5" modified="2017-09-27" version="1">MKIKRRLYSLIPLVLLFVLLWRLDHRTLLLIPLGLMAIQWYFIGTLFLVSVGAYLIYTKTGGLYGLVVMSLAVMAIELGYLDRERSPLEHYAILIAVTLLAFPTYLAMTSLSPVLPRLEVTAIAALLLIVLYLFARLATD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P639</accession>
    <name>A0A218P639_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CBS domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_02425</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853525.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P639"/>
    <dbReference type="GeneID" id="33315090"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02425"/>
    <dbReference type="OrthoDB" id="8919at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02205">
        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="51"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="129"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="184" mass="21293" checksum="8763D94A9A385CFE" modified="2017-09-27" version="1">MEDKSSDKAKNSKAKKIHIIHSKRRLIQMKRKEELSHNIRYISKVPVKLVMDTEFLTLHPNDSLSKLIQHLRGEESSAVVVDEEGRLLGFITMKDLLHYFEPPRRYSVVGLNLLKKYSLNRTSRVEDLMVRTPVTIGVDDDLGRAIQVMLETGKHHLPVIDKERKVHGLLEVKDIIRLIRIVSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P648</accession>
    <name>A0A218P648_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853535.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P648"/>
    <dbReference type="GeneID" id="33315100"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02475"/>
    <dbReference type="OrthoDB" id="102209at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43471">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43471:SF12">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12679">
        <property type="entry name" value="ABC2_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="253"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="302"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="346"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="386"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="417"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="489"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="517" mass="56186" checksum="F569B0A4E3F70F72" modified="2017-09-27" version="1">MKKPGVMSRLNGQWIALVLISLFPAIGVEIDVHRFTIHYPNIPPEELGHLITADLLTKWLPNFIRFLLILVLLIILLSRFGSDFERGNVVLMLSKPITRRRYFLGRSLEGLKLALTSALGIVLSGALAMFAHGFEVRDYLIGSLTLSLSLVGVIGIALLLLPLATSRDSGVFLGLGAFVALLLLGKSDYPFIPTVYLEKVLSIGDSVSIFYRAVGELLALCVALSLAGMELFRRRELRNPELLSAPGVSISPCGLYGVFLGLSLQSRRFIAFVAFTGLMAFLNKGTLDQYYTSHGIPGLLNAVIQAFNSPFLPFVVLPLGALSIGSAIENGTVRVLLSKPMKRKNFFLGMLLSDIFAVFIGTAFYVTLIVAYALHLSAPLSRTLELGLTFGSLLFLSLLQYLALGYLLSAFMRGRKALLLSLVLAFLLAFAVPLSVIAASASAGNSFIDVLTQNSLPVPSPNLHYTVLTMAVSPKRSLLPKSPAEVLNYPGNLAVLVVPTVAYLAISWLKFRKTDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P677</accession>
    <name>A0A218P677_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_02535</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853547.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P677"/>
    <dbReference type="GeneID" id="33315113"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02535"/>
    <dbReference type="OrthoDB" id="84861at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21913">
        <property type="entry name" value="Nip7_N_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21151">
        <property type="entry name" value="PUA_Nip7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040598">
        <property type="entry name" value="NIP7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005155">
        <property type="entry name" value="UPF0113_PUA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17833">
        <property type="entry name" value="pre-PUA_NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03657">
        <property type="entry name" value="UPF0113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="60S ribosome subunit biogenesis protein NIP7 pre-PUA" evidence="2">
        <location>
            <begin position="7"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="UPF0113" evidence="1">
        <location>
            <begin position="97"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="168" mass="19875" checksum="385F004E219F2982" modified="2017-09-27" version="1">MERELKYRRASAWEYDLILREAEKYGELRHHTFAVVEGRFRDVYAVNERVWREIEGLKMKPYAYGTFVGTIKVDKNLVEKFYPNVEFFYFVDVQKNYAVLTPKAGFLFTTGKDVPRSGVRSYNWQGTKKLVIYDENGIILGIGRINPESRKKFILNVTDIGEFIRRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6F9</accession>
    <name>A0A218P6F9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4350 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_02985</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853630.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6F9"/>
    <dbReference type="GeneID" id="33315203"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02985"/>
    <dbReference type="OrthoDB" id="372296at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025646">
        <property type="entry name" value="DUF4350"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14258">
        <property type="entry name" value="DUF4350"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4350" evidence="2">
        <location>
            <begin position="36"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="332" mass="37727" checksum="6BAF1D4ED6BD746C" modified="2017-09-27" version="1">MNKTVKYLILLLMLFTLITMPLTIPVFKSSTQYSIFNSGWDGTSRFVKLAYLKGKNVVPIFESFDIANISELNGVLIIIGPNMTFTGAEVEQIKLFLERGNTLFIADDFGTGNEILRALNVPVGISDYPLWDFFYEGDDRLVVSVRIEDPLLARNVTKVVTNEPSGIIVTRKGEAYVSKVAMINLHRRMYPIMTELRYGNGRIVVLSDPDVLANMQFEENKQFLSNLIDYLGGDVFYFDEAHHPDFNLYTVGTVTITRVLPKDRAVKLILAVAALILLKELGVFGLLGRFIRRFASRFFRKEINVDEVALSLAREKGWDENEVREMLMRMGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6K1</accession>
    <name>A0A218P6K1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="12">5-formaminoimidazole-4-carboxamide-1-(Beta)-D-ribofuranosyl 5'-monophosphate synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">A3L08_03260</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853676.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6K1"/>
    <dbReference type="GeneID" id="33315258"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03260"/>
    <dbReference type="OrthoDB" id="84677at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006188">
        <property type="term" value="P:IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023656">
        <property type="entry name" value="IMP_biosynth_PurP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009720">
        <property type="entry name" value="IMP_biosynth_PurP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010672">
        <property type="entry name" value="IMP_biosynth_PurP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38147:SF1">
        <property type="entry name" value="5-FORMAMINOIMIDAZOLE-4-CARBOXAMIDE-1-(BETA)-D-RIBOFURANOSYL 5'-MONOPHOSPHATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38147">
        <property type="entry name" value="5-FORMAMINOIMIDAZOLE-4-CARBOXAMIDE-1-(BETA)-D-RIBOFURANOSYL 5'-MONOPHOSPHATE SYNTHETASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06849">
        <property type="entry name" value="DUF1246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06973">
        <property type="entry name" value="DUF1297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004602">
        <property type="entry name" value="ATPgrasp_PurP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMP biosynthesis enzyme PurP N-terminal" evidence="10">
        <location>
            <begin position="21"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="IMP biosynthesis enzyme PurP C-terminal" evidence="11">
        <location>
            <begin position="189"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="380" mass="43626" checksum="A42FE4C7978F289E" modified="2017-09-27" version="1">MISRDEILGVLEKYDPERITVGVIGSHSALDIADGAKEEGLPVLVVAQRGRHRTYAEYFKLRKTRDGLTKGFIDEVIVLDKFAQIIDVQDELIKRNVIFVPNRSFVVYTGIDRVENDFRVPLFGSRNLLRSEERSEEKSYYWLLEKASLPYPEEVKPEEIDDVGLVIVKLPHAKKRLERGFFTAASYKEFREKAERLKKLGVITEEDLARARIERYIIGPVFNFDFFYSPIDGKIELLGIDWRFETSLDGHVRLPASQQLTLPEHQFEPEYTVCGHASSTLRESLLEKVFDMAERYVKATQEYYPPGIIGPFTLQTAVDKDLNFYIYDVAPRTGGGTNIHMAMGHPYGNALWRMPMSTGRRVALEIKRAIELDELEKVVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6P6</accession>
    <name>A0A218P6P6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853689.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6P6"/>
    <dbReference type="GeneID" id="33315273"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03335"/>
    <dbReference type="OrthoDB" id="86127at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="209" mass="23853" checksum="D999F1F5DA51BB88" modified="2017-09-27" version="1">MTDPVRDSVEVALDLDEKEVYSHIAHESAEDIIRIISSLDAERAKLHGEVIYYRDDWDDLIRERIAKGKRHTAFDFYNPALLDIWEAKVQEIKEIRKRERLGYTALGIVIAAAGAISVFTGQPYVLLGLALLPLAVLLHDRAKERFDLIYYELTQLFIDELRNLVGGHGLQPEHYKFKIFSGDYFGIETKKLGTGLFAVVKVEKSKGNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6S1</accession>
    <name>A0A218P6S1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03675</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853746.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6S1"/>
    <dbReference type="GeneID" id="33315340"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03675"/>
    <dbReference type="OrthoDB" id="358785at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42731:SF4">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42731">
        <property type="entry name" value="SLL1084 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Elp3/MiaA/NifB-like radical SAM core" evidence="1">
        <location>
            <begin position="215"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="536" mass="61746" checksum="84CAD92681D2A868" modified="2017-09-27" version="1">MARIVLTTDETLTSTYHDVPLLDFLGCAPYDKLPKWVFRFFDTQLPDENGVLSQAPYGLRKVEAALLRDGFNRDEVVVAHPRAVERFIDGKTTIVALYEMDPLGLGPVSMMFTNGGQWRNYTSVKFRELINRINRVREAKGLKFKLVVGGPGAWQLEFRREEREKLRIDHVVMGEVDHVAGEIFRDIESGSADEAVFVRGWPRVEQIPTIVAPSYKGLVEVMRGCGRGCRFCEPNLRVARYIPIEKIEEEIRLNVNAGIDHAWLHSEDVFLYRVEDKKNFYPNAEAVVELFEVARRYTRNVNPTHGSVAGALAIPDMIERISEIVDAGPNHWVGIQVGFETASPELIGKYMNNKMKPFSPEEWPWVLLNGTYVFNKNYWFPAYTTILGLPGDTDEYEIMTARLIVTMEKELEEKLGNRAHFTVTPLAFVPMGLLKDQEFYKIDDMITYGQFLHLYYAWKHMMREVTRGLPAVMKNNPFLIPFYPLARLGTRIVIRQIEKWGKSKGYEVKRLEPLEGLRIEVDEHRWYSKPALAEAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6T0</accession>
    <name>A0A218P6T0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03510</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853719.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6T0"/>
    <dbReference type="GeneID" id="33315307"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03510"/>
    <dbReference type="OrthoDB" id="93734at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="85" mass="9812" checksum="88E70307EAD97B15" modified="2017-09-27" version="1">MAERILEVLKTKYDFLSIMLQGLEGAIEDISNETDPHEVYRTLVRYLGEFPTRAMLQKMADEKGLGIRVRTEEDVIRAIELVSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6U0</accession>
    <name>A0A218P6U0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MFS transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_03750</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853760.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6U0"/>
    <dbReference type="GeneID" id="33315355"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03750"/>
    <dbReference type="OrthoDB" id="56622at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520:SF5">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="335"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="12"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="391" mass="43088" checksum="9D4CA0F2B743F93D" modified="2017-09-27" version="1">MSLQNYRGFSRDAWLLVAYSFVSWLGDNIAWFIFPFYLKSLSYDYTGIGMVFSLSTLAQASVLLFSGPLGARIGYKRAVLMGISFMFFGRLVQVLYPALIPLVLGGVLLGVGMAFEGPSFMALLSGEVTDVKRHYLFSLSSAVGTIGSAIGLVLAGFLPRYLTYREVFSLVLFIIPVRFVLVAVVKSVLAHSEKSLNLNRELLFRIGRFALPSALIGLGAGVTIPYVGLWFNQRFGTSLESIGWLFALQQFIMGLGTFLLPMIADRLGSVKTIVSFNGSASVLIAAMPLSPTFYIAAAVYTVRTILMNIVSPIWNSFMMSLFKKEERSTAMALNNLSWTATFGAGQYLGGILFDVSLTWPFMITALLYALSMVFFWGFFRGETKGYKPSRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6U5</accession>
    <name>A0A218P6U5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03760</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853762.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6U5"/>
    <dbReference type="GeneID" id="33315357"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03760"/>
    <dbReference type="OrthoDB" id="60382at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="84" mass="9638" checksum="1F4E59F26A89C44F" modified="2017-09-27" version="1">MKIVLEVTFRMGGVSYRGHRWEDDALVFEFERLGDAFIQVLSTRKVEEEVEKAPSKVLVELRTKEGGKVFEATETDGVYRAMEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6W7</accession>
    <name>A0A218P6W7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exosome complex component Rrp4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rrp4</name>
        <name evidence="8" type="ORF">A3L08_03855</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Non-catalytic component of the exosome, which is a complex involved in RNA degradation. Increases the RNA binding and the efficiency of RNA degradation. Confers strong poly(A) specificity to the exosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the archaeal exosome complex. Forms a trimer of Rrp4 and/or Csl4 subunits. The trimer associates with an hexameric ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the RRP4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853781.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6W7"/>
    <dbReference type="GeneID" id="33315376"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03855"/>
    <dbReference type="OrthoDB" id="35160at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071034">
        <property type="term" value="P:CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22524">
        <property type="entry name" value="KH-I_Rrp4_prokar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05789">
        <property type="entry name" value="S1_Rrp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00623">
        <property type="entry name" value="Exosome_Rrp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026699">
        <property type="entry name" value="Exosome_RNA_bind1/RRP40/RRP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023474">
        <property type="entry name" value="Rrp4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054371">
        <property type="entry name" value="RRP4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048565">
        <property type="entry name" value="S1_RRP4"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003181">
        <property type="entry name" value="PRK04163.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321:SF4">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321">
        <property type="entry name" value="PNAS-3 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22625">
        <property type="entry name" value="ECR1_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15985">
        <property type="entry name" value="KH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21266">
        <property type="entry name" value="S1_RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110324">
        <property type="entry name" value="Ribosomal L27 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0271">Exosome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="7">
        <location>
            <begin position="65"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="256" mass="28535" checksum="5149231BAE416EB3" modified="2017-09-27" version="1">MKRIFVKPRELVVPGTLLAQGPFKNGRGTFREGNRIYSTVVGLVEVRGDAIRVIPLEGPYMPEVGDNVLGKIVDVRFSNWVVDIGAPYEASLRVQDATEERIDLMKTDLRKIFDIGDIIYARIKGYNEINQIDLTTKGMPFHGGPLRGGQLVTITPSKVPRLIGKGGSMINLIKKLTNTRIIVGQNGWVWVSGKNDELEKLAIEAILKVNRESHTQGLTDRIKELLTTRLQELKEQGVIEEIPQIEEPKNGEGEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6X9</accession>
    <name>A0A218P6X9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Acetyl-CoA carboxylase biotin carboxyl carrier protein subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_03905</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853791.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6X9"/>
    <dbReference type="GeneID" id="33315386"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03905"/>
    <dbReference type="OrthoDB" id="31083at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000003">
        <property type="entry name" value="Acetyl-CoA carboxylase biotin carboxyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050709">
        <property type="entry name" value="Biotin_Carboxyl_Carrier/Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004173">
        <property type="entry name" value="PRK05641.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45266">
        <property type="entry name" value="OXALOACETATE DECARBOXYLASE ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45266:SF3">
        <property type="entry name" value="OXALOACETATE DECARBOXYLASE ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0092">Biotin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="4">
        <location>
            <begin position="79"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="54"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="157" mass="16192" checksum="74A2768BB9B9E614" modified="2017-09-27" version="1">MAKVKVIVDGIEYEVEVEELPGGRFKVSFEDKEYTVEAKGLGIDFEALSAPVQASTPAPTATPSAPSPAPAPVAPAAPAAPTPAPAGEGVVTAPMPGKILRILVKEGEQVKTGQGLLILEAMKMENEIPAPKDGVVKKILVKEGDTVDTGQALIELG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Z6</accession>
    <name>A0A218P6Z6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rps17</name>
        <name evidence="9" type="ORF">A3L08_04095</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853824.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Z6"/>
    <dbReference type="GeneID" id="33315423"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04095"/>
    <dbReference type="OrthoDB" id="10698at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00364">
        <property type="entry name" value="Ribosomal_uS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.1000:FF:000005">
        <property type="entry name" value="30S ribosomal protein S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01345_A">
        <property type="entry name" value="Ribosomal_uS17_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_uS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028333">
        <property type="entry name" value="Ribosomal_uS17_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019978">
        <property type="entry name" value="Ribosomal_uS17_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019979">
        <property type="entry name" value="Ribosomal_uS17_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006345">
        <property type="entry name" value="PRK08572.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03630">
        <property type="entry name" value="uS17_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744:SF9">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00973">
        <property type="entry name" value="RIBOSOMALS17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00056">
        <property type="entry name" value="RIBOSOMAL_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="111" mass="12922" checksum="67CAB492A11AA7A5" modified="2017-09-27" version="1">MREIGLKVQPPAEKCDDPHCPWHGHLKIHGRYFEGVVVSDKGKKTVVVERQHYHYLKKYERYELRRSKIHAHNPECIDAKVGDRVLIAETRPISKTKSWVVVAVTKRAGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P714</accession>
    <name>A0A218P714_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein eL32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">rpl32e</name>
        <name evidence="6" type="ORF">A3L08_04135</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL32 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853831.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P714"/>
    <dbReference type="GeneID" id="33315431"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04135"/>
    <dbReference type="OrthoDB" id="372100at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00513">
        <property type="entry name" value="Ribosomal_L32_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00810">
        <property type="entry name" value="Ribosomal_eL32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001515">
        <property type="entry name" value="Ribosomal_eL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023654">
        <property type="entry name" value="Ribosomal_eL32_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018263">
        <property type="entry name" value="Ribosomal_eL32_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036351">
        <property type="entry name" value="Ribosomal_eL32_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006332">
        <property type="entry name" value="PRK08562.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L32 AND DNA-DIRECTED RNA POLYMERASE II, SUBUNIT N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01655">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01393">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52042">
        <property type="entry name" value="Ribosomal protein L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00580">
        <property type="entry name" value="RIBOSOMAL_L32E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="126" mass="14794" checksum="D219272E376EFA8B" modified="2017-09-27" version="1">MNEKARLLRIRARIKRKKPRFLRQEWWRYPKFKNNPKWRRPKGIDSKMRLKKKGKPRSPSIGWSSPRAVRGLHPSGYEEVLVHNVRELEAIDPSRQAARIARTVGARKREAILAKAKELGVKVLNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P715</accession>
    <name>A0A218P715_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Small ribosomal subunit protein uS8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rps8</name>
        <name evidence="10" type="ORF">A3L08_04125</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 9">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088179855.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P715"/>
    <dbReference type="SMR" id="A0A218P715"/>
    <dbReference type="GeneID" id="33173731"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04125"/>
    <dbReference type="OrthoDB" id="5670at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.30:FF:000001">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01302_A">
        <property type="entry name" value="Ribosomal_uS8_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_uS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047863">
        <property type="entry name" value="Ribosomal_uS8_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_uS8_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003115">
        <property type="entry name" value="PRK04034.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S15A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="Ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 8 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="130" mass="14604" checksum="6C51F2D9A60D571C" modified="2017-09-27" version="1">MTLLDPLANALSHITNSERVGKKEVYLKPASKLMGEVLRVMQENGYIGEFEFIDDGRAGIYRVQLIGKINKAGAIKPRFPVKAREYEAWEKRFLPAFEFGILIVSTSQGVMTHKEAIEKGIGGRLIAYVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P753</accession>
    <name>A0A218P753_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Prenyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_04345</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853865.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P753"/>
    <dbReference type="GeneID" id="33315473"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04345"/>
    <dbReference type="OrthoDB" id="100801at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="433" mass="48725" checksum="4FE5742CA013D845" modified="2017-09-27" version="1">MPTEVYREALRLAEDIRDKYLRAVTYAKIGYYMHRAKHPEYKTAFKYAFNVVSSMENPFLAAKALIEIGTYLWKTGSKTARKVFGQVHDAVVDFPQPIRDDLLEELVVRLLELGIIDDALFYVGDIEDNIKRNDLLLKILRVYLKHGNMRRAYLIINQIDDEPWRSIAVVETVKVHLKREEFGSAIRILTELKSEYWLGEAMKEVAMCLKTADVPKATYQKFVDIALSLSGETGSDVLNSLLIGMGSQGELEFVMGILEKLPPEQRTSVLEGIVSASIDREEVLSRLLNLLRGDEFERIAGFVMDRLLSRPVSESYSDLVKTIGGRTREDAVLVKVATYLAKLGDFDAALEFASRVRGHYLRSLAFGSIAVSKLKGGDIDGAIDAALEVKDPKWGSWLLSEILTKILELQAEGEVSEDIEAKAESQRAIWEKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P767</accession>
    <name>A0A218P767_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein eL18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl18e</name>
        <name evidence="7" type="ORF">A3L08_04240</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853848.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P767"/>
    <dbReference type="GeneID" id="33315452"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04240"/>
    <dbReference type="OrthoDB" id="11309at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.100.10.10:FF:000013">
        <property type="entry name" value="50S ribosomal protein L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00329">
        <property type="entry name" value="Ribosomal_eL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000039">
        <property type="entry name" value="Ribosomal_eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021132">
        <property type="entry name" value="Ribosomal_eL18/eL18-A/B/_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022947">
        <property type="entry name" value="Ribosomal_eL18_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003079">
        <property type="entry name" value="PRK04005.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17135">
        <property type="entry name" value="Ribosomal_L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01106">
        <property type="entry name" value="RIBOSOMAL_L18E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="6">
        <location>
            <begin position="12"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="121" mass="13834" checksum="3F0AF40E90775929" modified="2017-09-27" version="1">MVKRTGPTDVNLRRLIRYLRKKSNEEGVRIWKDIAWRLERPRRQRAEVNVSRINRYTKEGDIVIVPGSVLGAGKLEHKVTVAAWKFSETAKKKIVEAGGEVLTIEELIERNPKGSGVIIME</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P769</accession>
    <name>A0A218P769_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tryptophan synthase beta chain</fullName>
            <ecNumber evidence="12">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">trpB</name>
        <name evidence="14" type="ORF">A3L08_04470</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 12">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the TrpB family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="12"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853884.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P769"/>
    <dbReference type="GeneID" id="33315498"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04470"/>
    <dbReference type="OrthoDB" id="371827at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052684">
        <property type="term" value="F:L-serine hydro-lyase (adding indole, L-tryptophan-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00133">
        <property type="entry name" value="Trp_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006316">
        <property type="entry name" value="Trp_synth_b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006653">
        <property type="entry name" value="Trp_synth_b_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009057">
        <property type="entry name" value="PRK12391.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01415">
        <property type="entry name" value="trpB_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077:SF6">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001413">
        <property type="entry name" value="Trp_syn_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500824">
        <property type="entry name" value="TrpB_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00168">
        <property type="entry name" value="TRP_SYNTHASE_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 12" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="13">
        <location>
            <begin position="75"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="12">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ06628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="441" mass="49150" checksum="30716C835138631C" modified="2017-09-27" version="1">MKAVLPDSKIPTKWYNILPDLPEPLEPPLDPETDEPMEPEKLLRIFAAELVKQEISTERYIEIPKKVRELYAKIGRPTPLFRATNLEKVLGTPARIYFKYEGATVTGSHKINTALAQAYYAKEQGIERLVTETGAGQWGTALSLAGALLGLKVRVYMARASYRQKPYRKTIMRLYGAEIYPSPSDRTEIGRKFLAEDPNHPGGLGIAISEAIKDVLRDEKARYSLGSVLNHVLMHQTVIGLEAKEQMKEFEEPDVIIGCVGGGSNFAGLAYPFVKDVLHGKADYEFITVEPRAAPSMTRGVYKYDFGDSGGYTPKMKMHTLGHTYYVPPIHAGGLRYHGLAPTLSVLINHGVVKPVAYHQNEVFQAAELFAKTEGIIPAPESAHAIKGAIDRALNAKEERKEEVILFNLSGHGFLDLQGYEDYLDRKLEDYEPDYFPALEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7A8</accession>
    <name>A0A218P7A8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRISPR type III-associated protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_04690</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853925.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7A8"/>
    <dbReference type="GeneID" id="33315542"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04690"/>
    <dbReference type="OrthoDB" id="102045at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRISPR type III-associated protein" evidence="3">
        <location>
            <begin position="32"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="310"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="329" mass="35905" checksum="A764743E68C45D20" modified="2017-09-27" version="1">MRVILKMKALEPISVGYESIGIETLLYSLPIVKEDGTALEVPVIPGNSLRGVLRDQMALQFLEDVKSAILESKNETEAPEKQESLNAHIGTLLSLFSGGILSGEEKAQGMPNIKEIMEKYVYPLLPLSIMGVALKKIIIPGKIKVGIGYPVTEETKPLLTDLGVSPNDSAPALSEITASVLITRKDDTNKVMQLKDLLNVEGEVEEAKAGDSAVQQRLYRQVVVPGTVFYSYVEDVIPMTDAEWGLVLKTLERLDKIGGRVAGGLGKVKIELSGIDNEKREAFIEAYTNYIGDNLNTILKALKTNPNDFFKSSPNSSESVKTNEKSPET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7B3</accession>
    <name>A0A218P7B3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HPt domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_04635</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853914.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7B3"/>
    <dbReference type="GeneID" id="33315531"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04635"/>
    <dbReference type="OrthoDB" id="26305at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008207">
        <property type="entry name" value="Sig_transdc_His_kin_Hpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009563">
        <property type="entry name" value="SSSCA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001645">
        <property type="entry name" value="PRK00420.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06677">
        <property type="entry name" value="Auto_anti-p27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50894">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HPt" evidence="3">
        <location>
            <begin position="61"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="51"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="107" mass="12466" checksum="53BBBD33D98432F3" modified="2017-09-27" version="1">MKGPTEWEMRNIIMPLLLSGAKMLDRHCPKCGSPLFEKDGRVFCPVCEHRAKQEKSEMRGVEERLKEKLSELANSLPDDIDELEKHLRAMEKIIELLERYRKLEGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7F6</accession>
    <name>A0A218P7F6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_04980</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853971.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7F6"/>
    <dbReference type="GeneID" id="33315600"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04980"/>
    <dbReference type="OrthoDB" id="98427at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="253" mass="27741" checksum="BA8E772857263761" modified="2017-09-27" version="1">MRRAYLGLLIAFIVFAAGCIFGGSSQTGTPSTTTSSSLPFTAEELKNAITGLKSYEYAVRVDSYNGTKLAAQLFTNGSVDFERKLKSVLTFSNTTSGGGAYYRSYYYTTARGYAAYFDRNGTVSWEASCYGPGQGPGFNSTSLDWLSDIISVDGVKVVEKDEYYIVYANETGGTAGNNQITAYKTEVVVKLTKELIPVEVNTTVHYKRDGFDWVDVTRMVISRPNAAKVEPPEELVSYLEKQGISLEEFLKGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7H1</accession>
    <name>A0A218P7H1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycerol kinase</fullName>
            <ecNumber evidence="9">2.7.1.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP:glycerol 3-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Glycerokinase</fullName>
            <shortName evidence="9">GK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">glpK</name>
        <name evidence="13" type="ORF">A3L08_04945</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>glycerol + ATP = sn-glycerol 3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21644"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.30"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the FGGY kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.30" evidence="9"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853965.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7H1"/>
    <dbReference type="GeneID" id="33315593"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04945"/>
    <dbReference type="OrthoDB" id="26592at2157"/>
    <dbReference type="UniPathway" id="UPA00618">
        <property type="reaction ID" value="UER00672"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07786">
        <property type="entry name" value="FGGY_EcGK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000007">
        <property type="entry name" value="Glycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000008">
        <property type="entry name" value="Glycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00186">
        <property type="entry name" value="Glycerol_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000577">
        <property type="entry name" value="Carb_kinase_FGGY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018483">
        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005999">
        <property type="entry name" value="Glycerol_kin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01311">
        <property type="entry name" value="glycerol_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000756">
        <property type="entry name" value="PRK00047.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196:SF69">
        <property type="entry name" value="GLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000538">
        <property type="entry name" value="GlpK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00933">
        <property type="entry name" value="FGGY_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00445">
        <property type="entry name" value="FGGY_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="5 9 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="11">
        <location>
            <begin position="4"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="12">
        <location>
            <begin position="256"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>sn-glycerol 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>glycerol</name>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>sn-glycerol 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>glycerol</name>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>sn-glycerol 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>glycerol</name>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>sn-glycerol 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>glycerol</name>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>sn-glycerol 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>glycerol</name>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="406"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00052101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="493" mass="55667" checksum="037725F2D2C743D4" modified="2017-09-27" version="1">MERYILSLDEGTTSARAIIFDRESDIPGIGQYEFPQHYPRPGWVEHNPEEIWEAQMRAIKTAIERAKIEPNQIAAIGVTNQRETTIVWDRSGRPLYNAIVWQCRRTAEMVEEIKREYGNIIKEKTGLVPDAYFSASKLKWLFDNVPGLREKAEKGEVLFGTVDTFLIYRLTGEHVTDYSNASRTMLFNIRRLEWDDELLEIFDIPESVFPEVRESSEVYGYTKKELLGAEIPVSGDAGDQQAALFGQAAFETGMVKATYGTGSFILANTGKMVRYSENLLTTIAWGLNGKVSYALEGSIFVTGAAVQWLRDGIKIIKSAAETEELAAKLESNEGVYFVPAFVGLGAPYWDQFARGLIIGITRSTGREHFARATLEAIAYLTRDVVEEMEKLVGIKELRVDGGATANDFLMQFQADILNRRVIRPVVKETTALGAAYLAGLAVDYWSGLDEIKSLWKAERVFEPKMDEETRERLYRGWKEAVKRAMSWAKVVDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7I6</accession>
    <name>A0A218P7I6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CYTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_05080</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853986.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7I6"/>
    <dbReference type="GeneID" id="33315620"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05080"/>
    <dbReference type="OrthoDB" id="101695at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023577">
        <property type="entry name" value="CYTH_domain"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51707">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CYTH" evidence="2">
        <location>
            <begin position="10"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="3"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="65" mass="7972" checksum="F3E0D516AC65B20E" modified="2017-09-27" version="1">MESVDLIRELMRIEKKLEDIEKLLNELLEREESYALMKLSEESLKEFFTDEPDIYSEEDLKVRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7K0</accession>
    <name>A0A218P7K0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Phosphoglucosamine mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_05175</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854000.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7K0"/>
    <dbReference type="GeneID" id="33315639"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05175"/>
    <dbReference type="OrthoDB" id="10363at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008966">
        <property type="term" value="F:phosphoglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03087">
        <property type="entry name" value="PGM_like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000001">
        <property type="entry name" value="Phosphoglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000003">
        <property type="entry name" value="Phosphoglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024086">
        <property type="entry name" value="GlmM_arc-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03990">
        <property type="entry name" value="Arch_GlmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43771">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43771:SF1">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="8">
        <location>
            <begin position="3"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="9">
        <location>
            <begin position="156"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="10">
        <location>
            <begin position="261"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase C-terminal" evidence="7">
        <location>
            <begin position="384"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="456" mass="49951" checksum="7F76B8C2AB09C47F" modified="2017-09-27" version="1">MGKLFGTFGVRGIANEKITPEFALKMGMAFGTMLKREGRKRPLVVVGRDTRVSGEMLKDALISGLLSVGCDVIDVGIAPTPAIQWATANFNADGGAVITASHNPPEYNGIKLLEPNGMGLKKEREAVVEEVFFKEDFDRARWDEIGEVREENIIKPYIEVIKSRVDIEAIRKRKPFVVVDTSNGAGSLTLPYLLRELGCKVVSVNAHPDGHFPARNPEPNEENLKEFKEIVKALGADFGVAQDGDADRAVFIDENGRFIQGDKTFALVADAVLRENGGGLLVTTIATSNLLDDIAKRNGAKVMRTKVGDLIVARALLENNGTLGGEENGGVIFPDFVLGRDGAMTTAKIVEIFAKSGKKFSELIDELPKYYQFKTKRHVEGDRKAIVAKVAELAEKRGYRIDTTDGTKIIFDDGWVLVRASGTEPIIRVFSEAKSEEKAKEYLNLGLELLEEALRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Q8</accession>
    <name>A0A218P7Q8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854053.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Q8"/>
    <dbReference type="GeneID" id="33315694"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05450"/>
    <dbReference type="OrthoDB" id="97457at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="263"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="307" mass="34154" checksum="209DBA2FD5284DCF" modified="2017-09-27" version="1">METHEVELGQTLRSKKLLALLLISVLLTYAVPFPFIAGFLEDYGKIKEIKKQMEEENEAIVCHTVYDTDPEFFGSQGMECSDENYRPNESTVSCCGMSVYRDTYQEYLRAREDLGKELPKFVLFVVWVFLSLLAFSYALVEGAVSITNGGEVSLKRSILSGFRALPALVASEFVMFVAIIAVLILLAVPMAILGPLGGILVVILTAPAFALVIPVYYFERKIGPVGEIWRIFKNNTGGYLVLGLLAGIVDLLVAFQYEYYFGIGTLLIMLAIGIPRYVLNSVGALVVYLDGVETLEREEKRGIEWEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7R9</accession>
    <name>A0A218P7R9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7R9"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05630"/>
    <dbReference type="OrthoDB" id="100008at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="108" mass="11423" checksum="9683213259342C97" modified="2017-09-27" version="1">MAENEDGLRVALYISTIITGLIGIITVVPLVAELYLFHVLKRVDAKITDEGVLASFSAINVLLAVAVHSNDPNVDPLLPTIITALTFLFLAIKLESGAGNEVCGDHPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7T7</accession>
    <name>A0A218P7T7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF357 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854051.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7T7"/>
    <dbReference type="GeneID" id="33315692"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05440"/>
    <dbReference type="OrthoDB" id="148073at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.90">
        <property type="entry name" value="AF1782-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036809">
        <property type="entry name" value="AF1782-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023140">
        <property type="entry name" value="DUF357"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04010">
        <property type="entry name" value="DUF357"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158372">
        <property type="entry name" value="AF1782-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF357" evidence="1">
        <location>
            <begin position="12"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="95" mass="11003" checksum="577607C3D1ADA8C1" modified="2017-09-27" version="1">MGREITEEKLQKYFRITEEALRTLEVAVHEKSLLWVAAQDFLTMAKSYFEDAKYYYEKGDYVTAFAALNYAHGFIDAGVRLGVFRGEDDRLFAFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7W1</accession>
    <name>A0A218P7W1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Radical SAM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_05765</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854112.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7W1"/>
    <dbReference type="GeneID" id="33315757"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05765"/>
    <dbReference type="OrthoDB" id="5682at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027596">
        <property type="entry name" value="AmmeMemoSam_rS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034457">
        <property type="entry name" value="Organic_radical-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016431">
        <property type="entry name" value="Pyrv-formate_lyase-activ_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04337">
        <property type="entry name" value="AmmeMemoSam_rS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352:SF5">
        <property type="entry name" value="PYRUVATE FORMATE-LYASE 1-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352">
        <property type="entry name" value="PYRUVATE FORMATE-LYASE-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004869">
        <property type="entry name" value="PflX_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01101">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="67"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR004869-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="348" mass="40272" checksum="0B3FBC6BCCC27F09" modified="2017-09-27" version="1">MREAMYWEPLEGGKVRCKLCPLNCIINEGQRGSCRIRKNIGGKLYTLNYGKVSAIGTDPVEKKPLFHFWPGSCALSISTVGCNMHCKHCQNWEISQADESFPYLHDMTPEMVVAITKRYGCESIAYTYNEPTIWYEFVLDTARLAKKEGIYNLMITNGYINEEPFRELAPYIDAMNIDIKAFNDKFYMKIASVPSGEPSRRTAIIAKREYGIHVELTYLIIPTLNDNEEEIRAFARWVVENLGDDTPVHFSRFFPHYKLNHLSPTPIKTVEMAYRIAKEEGLKFVYIGNVPGHEGENTYCPRCGKPLIVRWGFEITEYKIEDGKCKYCGEPIPIVGTYTKKRYNWMWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Y9</accession>
    <name>A0A218P7Y9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">7-carboxy-7-deazaguanine synthase</fullName>
            <shortName evidence="8">CDG synthase</shortName>
            <ecNumber evidence="8">4.3.99.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Archaeosine biosynthesis protein QueE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">queE</name>
        <name evidence="10" type="ORF">A3L08_06065</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>6-carboxy-5,6,7,8-tetrahydropterin + H(+) = 7-carboxy-7-carbaguanine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:27974"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:61032"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="EC" id="4.3.99.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </cofactor>
        <text evidence="8">Binds 1 S-adenosyl-L-methionine per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="8">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the radical SAM superfamily. 7-carboxy-7-deazaguanine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.3.99.3" evidence="8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854167.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Y9"/>
    <dbReference type="GeneID" id="33315816"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06065"/>
    <dbReference type="OrthoDB" id="7980at2157"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016840">
        <property type="term" value="F:carbon-nitrogen lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00917">
        <property type="entry name" value="QueE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024924">
        <property type="entry name" value="7-CO-7-deazaguanine_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42836">
        <property type="entry name" value="7-CARBOXY-7-DEAZAGUANINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42836:SF1">
        <property type="entry name" value="7-CARBOXY-7-DEAZAGUANINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000370">
        <property type="entry name" value="QueE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5 8" id="KW-0408">Iron</keyword>
    <keyword evidence="6 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="9">
        <location>
            <begin position="23"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="12"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="42"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="253" mass="28701" checksum="2FED2BAFD73A635B" modified="2017-09-27" version="1">MRLIMAEVFNSWQGEGGSVEGSAFGRRQIFVRFAGCDLHCQWCDSKEYIDASRVSRWRYEVEPFTGKFEYRPNPASVDEVVDAVLRLDTGDIHSISYTGGEPTLQVKPLKALMERMKELGFDNFLETHGGLPELIKEVAHLVNYASVDIKDESAGATDDWKDLVLREVESIRILKEAGAKTYAKLVVTSETKLENVRWYAELLNGLAPLVIQPREPIDVPQERLMEFYGEAARIMGRKNVGLSFQVHKYLNVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Z3</accession>
    <name>A0A218P7Z3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Aldehyde ferredoxin oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_05965</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>tungstopterin</name>
            <dbReference type="ChEBI" id="CHEBI:30402"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AOR/FOR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854152.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Z3"/>
    <dbReference type="GeneID" id="33315797"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05965"/>
    <dbReference type="OrthoDB" id="30771at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.569.10">
        <property type="entry name" value="Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.599.10">
        <property type="entry name" value="Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.9.10">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013984">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013985">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013983">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036503">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001203">
        <property type="entry name" value="OxRdtase_Ald_Fedxn_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036021">
        <property type="entry name" value="Tungsten_al_ferr_oxy-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051919">
        <property type="entry name" value="W-dependent_AOR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30038">
        <property type="entry name" value="ALDEHYDE FERREDOXIN OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30038:SF0">
        <property type="entry name" value="TUNGSTEN-CONTAINING ALDEHYDE FERREDOXIN OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01314">
        <property type="entry name" value="AFOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02730">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00790">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48310">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, C-terminal domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56228">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0826">Tungsten</keyword>
    <feature type="domain" description="Aldehyde ferredoxin oxidoreductase N-terminal" evidence="10">
        <location>
            <begin position="4"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="618" mass="68917" checksum="58846C37ACF46A90" modified="2017-09-27" version="1">MYGYHNRIARVNLTEGKVTYEELPDEVIRKFIGGKGLGYYLIYREVPPGTDPLSPANKFVFAPGGMAGLVPGSSKVIAVSKSPETRLISDSSGGDAFGPKLKGHFDALIIEGKAEEPVYLYIHDGKVEILSAEHLWGKGNYEVAKEIWKEHPKASMAMIGPAGEKLSRIANVVYDTERASGRGGLGAVLGSKKVKAVVVEPGEKPKVANPEEFQRLWQEYYNEFATNPKYEHTRNYGTTDGMRGSASVGMSPAYNFSRPYIPDELASKLAGDEVKKYEVEPEWFVHGKSCPIKCARYVEVEYKGRKIRVKPEYESIAMLGAATGVFNFPAVAYFNWLVNNLGLDSIATGATIAWLFEMVERGLIGEDEIGFPVKGFGDEEAEEKLIKLMAERKGIGAILADGVKRACERLGRGCEFAVHVKGMESPAWDPRGRRTYALSYATADVGASHLRGWPRPHQLPNQGPAKELVPSLIEGRDESYITDMLGTCKFVPYKLEDLARLYSIVTGEEWTVEELRRRAWGVESIARIHNALDWVTPPLDDTIPPRWWEPESDGPAKGNAAFIDYNDFLEARREFYRLRGWHEELGVPLPETMEELSLPEFKEDAEKALEVVKKRMGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P815</accession>
    <name>A0A218P815_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Electron transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854179.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P815"/>
    <dbReference type="GeneID" id="33315829"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06130"/>
    <dbReference type="OrthoDB" id="31576at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051790">
        <property type="entry name" value="Cytochrome_c-biogenesis_DsbD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31272:SF9">
        <property type="entry name" value="BLL1027 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31272">
        <property type="entry name" value="CYTOCHROME C-TYPE BIOGENESIS PROTEIN HI_1454-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="242" mass="26270" checksum="F9B4C7744C8688A5" modified="2017-09-27" version="1">MRSELKGLAIILLVSFGISSLALWALDMVSFLPTFFTLALTDSINPCTFVIYTMLLIALSVREISRRRLYLVGMSFVVAVYISYYLLGVGLLYLGSYIPLWAAGVFAVAFGLYTIATGLMERSRVGGKSKVRRKIFSSDATLAGAFLLGIVVSTTLLPCSAGSYLVYATIIAHTSRAMAFLLLGLYNLIFVLPLVMILVAMGSVTESKRFSQAMVRHSRELSVIAGILLIAIGIWVLTGVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P861</accession>
    <name>A0A218P861_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Single-stranded DNA endonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_06385</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854224.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P861"/>
    <dbReference type="GeneID" id="33315880"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06385"/>
    <dbReference type="OrthoDB" id="36101at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1640.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048515">
        <property type="entry name" value="DHH_CID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051673">
        <property type="entry name" value="SSDNA_exonuclease_RecJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30255">
        <property type="entry name" value="SINGLE-STRANDED-DNA-SPECIFIC EXONUCLEASE RECJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30255:SF3">
        <property type="entry name" value="SINGLE-STRANDED-DNA-SPECIFIC EXONUCLEASE RECJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21763">
        <property type="entry name" value="DHH_CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDH" evidence="1">
        <location>
            <begin position="27"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="DHH-CID" evidence="3">
        <location>
            <begin position="199"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="2">
        <location>
            <begin position="376"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="475" mass="52430" checksum="C3506A64F14C0803" modified="2017-09-27" version="1">MDKGAFLERAREGAELIKMHIELGHTIRLISHRDADGITAGAILAKAVAREGGTFQLSIVKQVSEDLIRELAEERREIYVFSDLGSGSMELIEKHLDFATVVVADHHPPEKESFSNDSHVLVNPVPFGANSVRDLSGSGVAYFVAKEMNEKNRDLAYIALVGSVGDMQEIDGTFHGMNLDIIEDGKELGILEVRKELRLFGRESRPLRQMLAYSTNPELPGITGDERNAIEWLRSKGFDPDVHYWQLREEEKRKLHDALVIHLIKHGAPKEAIDRLIGDVVVSPLYPEGDPRHEAREFATLLNATGRLNAGTLGVAICLGDEDAYKKAKKMLDDYKREQIEARKFIVQNWSMADEGEHAYVFYAGKNIRDTLVGIAANIAINAGLADPEKPVVVIADSEEDENLVKGSARTTEKALAKGYHLGEALKEVAEKLGGEGGGHAIAAGIRFPKGRIDEFIRLFNEALAKQVGREGSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P864</accession>
    <name>A0A218P864_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Small ribosomal subunit protein eS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rps3ae</name>
        <name evidence="5" type="ORF">A3L08_06395</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the eukaryotic ribosomal protein eS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854226.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P864"/>
    <dbReference type="GeneID" id="33315882"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06395"/>
    <dbReference type="OrthoDB" id="30639at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00359">
        <property type="entry name" value="Ribosomal_eS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001593">
        <property type="entry name" value="Ribosomal_eS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030838">
        <property type="entry name" value="Ribosomal_eS1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018281">
        <property type="entry name" value="Ribosomal_eS1_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003142">
        <property type="entry name" value="PRK04057.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11830">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01015">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01397">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01191">
        <property type="entry name" value="RIBOSOMAL_S3AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="200" mass="23100" checksum="65B7367F1F16A5C4" modified="2017-09-27" version="1">MAKGNPRKRAAATKDKWKSKEWYIVYAPDFFGSKEIGLTPADEPEKVIGRVIETTLKDLTGDFTKGQVKLYFQIYDVKGQNAYTKFKGHNLARSYIRSLVRRRTTRVDGIFNVTTKDGYKLRVMGMVIAYRRIQTSQERAIREIIREIIYKKASELNFKDFVLEAVSGKMAAEIAKEARRIYPIKRAEIRKIKVLAEPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P868</accession>
    <name>A0A218P868_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphomevalonate dehydratase small subunit</fullName>
            <shortName evidence="7">PMDh small subunit</shortName>
            <shortName evidence="7">PMDh-S</shortName>
            <ecNumber evidence="7">4.2.1.182</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_06355</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 7">Component of a hydro-lyase that catalyzes the dehydration of mevalonate 5-phosphate (MVA5P) to form trans-anhydromevalonate 5-phosphate (tAHMP). Involved in the archaeal mevalonate (MVA) pathway, which provides fundamental precursors for isoprenoid biosynthesis, such as isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(R)-5-phosphomevalonate = (2E)-3-methyl-5-phosphooxypent-2-enoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:78975"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:229665"/>
            <dbReference type="EC" id="4.2.1.182"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:78976"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">Heterodimer composed of a large subunit (PMDh-L) and a small subunit (PMDh-S).</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the AcnX type II small subunit family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.182" evidence="7"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854220.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P868"/>
    <dbReference type="GeneID" id="33315874"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06355"/>
    <dbReference type="OrthoDB" id="18062at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01356">
        <property type="entry name" value="AcnX_swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.10">
        <property type="entry name" value="Phosphohistidine domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00078">
        <property type="entry name" value="PMDh_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012016">
        <property type="entry name" value="PMDh-S-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002840">
        <property type="entry name" value="PMDh-S-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020794">
        <property type="entry name" value="PMDh_S"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003046">
        <property type="entry name" value="PRK03955.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36577">
        <property type="entry name" value="DUF521 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G00490)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36577:SF3">
        <property type="entry name" value="DUF521 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G00490)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01989">
        <property type="entry name" value="AcnX_swivel_put"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004966">
        <property type="entry name" value="UCP004966"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="3 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphomevalonate dehydratase small subunit-like" evidence="8">
        <location>
            <begin position="24"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00045120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00045299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="133" mass="13676" checksum="8C2D5B2D809BB6CE" modified="2017-09-27" version="1">MKLKGRKIVGGKAEGELIVSQKPLSFLGGVDPKTGIITDAESDIRGQSIAGKILAFPRGKGSTVGSYVIYALKKNGKAPKAIIVGEAETIVATGAIISGIPMVDGVDVSKLKSGAKVRVDADRGEVEVIESEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8C8</accession>
    <name>A0A218P8C8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2-oxoglutarate ferredoxin oxidoreductase subunit gamma</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_06755</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854291.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8C8"/>
    <dbReference type="GeneID" id="33315954"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06755"/>
    <dbReference type="OrthoDB" id="18183at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016903">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052554">
        <property type="entry name" value="2-oxoglutarate_synth_KorC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006323">
        <property type="entry name" value="PRK08537.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42730">
        <property type="entry name" value="2-OXOGLUTARATE SYNTHASE SUBUNIT KORC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42730:SF1">
        <property type="entry name" value="2-OXOGLUTARATE SYNTHASE SUBUNIT KORC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate/ketoisovalerate oxidoreductase catalytic" evidence="2">
        <location>
            <begin position="11"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="186" mass="20262" checksum="C81F0751176D9A48" modified="2017-09-27" version="1">MRKEVLFSGFGGQGVILASVILGRAAAVYEGLYAVQTQSYGPESRGGASKAEVVISDEPIDYPKTLEPDYAVFFSQEAYSKYLHTVKEGAKVIIEKDLVPHRDLEFEKKLDVIALPLTEIAEETTGLSLTMNILTLGILTAWTDVVSKEAIEKAVLDTVPKGTEEINKRALKKGFEIGEKAKAGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8G3</accession>
    <name>A0A218P8G3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sugar-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_06825</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BMP lipoprotein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854304.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8G3"/>
    <dbReference type="GeneID" id="33315968"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06825"/>
    <dbReference type="OrthoDB" id="26626at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06354">
        <property type="entry name" value="PBP1_PrnA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050957">
        <property type="entry name" value="BMP_lipoprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003760">
        <property type="entry name" value="PnrA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34296:SF2">
        <property type="entry name" value="ABC TRANSPORTER GUANOSINE-BINDING PROTEIN NUPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34296">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN MED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02608">
        <property type="entry name" value="Bmp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="ABC transporter substrate-binding protein PnrA-like" evidence="7">
        <location>
            <begin position="37"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="410" mass="44285" checksum="8428FBB39B1B8E50" modified="2017-09-27" version="1">MKKWLSLFLIGLLAISVVASGCISSGGEESKTGAIAIVYDVGGRGDLSFNDMAYLGASKAAKDFNLDLVELQSNSEDDYVENLRTLASEGKYAVIIAVGFMMTDAVKQVASEYPDQKFAIIDGSDPEMPDNVMMILFKENEGSALAGALAGLIAANDGKDKVGIVLGMEIPVLYKFEAGYRFGVKWAEDYYKQKEGKDVNIDVLYQYTGSFGDAAKGKAAAQAQLSQGAWVIYQVAGGTGLGVFDAVEEALKAQNKKMGPPFAIGVDSAQDWIKPGVIIASMTKRVDVGVYTAVKDAVEGTFKGGVVELGLEEGGVGLSTVDDVMAMFDSLPEDTQKQKLKDLGFNSRDELKQYLEQTRGQVPDWIWDAVKQLQDKIVSGEIKVPAPMDKDGIEKVRQAKTWQDMQELAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8G8</accession>
    <name>A0A218P8G8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Carbamoyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_06690</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NodU/CmcH family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854280.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8G8"/>
    <dbReference type="GeneID" id="33315941"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06690"/>
    <dbReference type="OrthoDB" id="42122at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd24100">
        <property type="entry name" value="ASKHA_NBD_MJ1051-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.20">
        <property type="entry name" value="Carbamoyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031730">
        <property type="entry name" value="Carbam_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038152">
        <property type="entry name" value="Carbam_trans_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003696">
        <property type="entry name" value="Carbtransf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051338">
        <property type="entry name" value="NodU/CmcH_Carbamoyltrnsfr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34847">
        <property type="entry name" value="NODULATION PROTEIN U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34847:SF1">
        <property type="entry name" value="NODULATION PROTEIN U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16861">
        <property type="entry name" value="Carbam_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02543">
        <property type="entry name" value="Carbam_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbamoyltransferase" evidence="2">
        <location>
            <begin position="87"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Carbamoyltransferase C-terminal" evidence="3">
        <location>
            <begin position="365"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="543" mass="60086" checksum="255DE8DF64BD2511" modified="2017-09-27" version="1">MILGIHDGHDAGAVLIDGDRLFAVNEERLNRVKKYRGFPELSLRKVLEMANASPEDVEIIAVAGVFRKLARLKELETNLRAVFGPDFKRKVLFVEHHLAHSASAYYTSGWRDALAVSIDAAGDGLSSSVYVARGGEMIRIAQSTYVDSLGDFYASVTELLGFKPMRHEGKVMSLAAYGRPKYDLSAIIELNGLTFENHLGVIGVEATRKLAELFDYPLRHAKEIANQMKRGKLDGVLQKKAIEIAASAQAHLEKLIEELGLRLKNRALPVAYAGGVAQNVKANAVLRHVLGDDNLWVFPAMDDGGLAFGAAVFVKAQMERLDGKWKPFRLEHVYLGPGYGPDYVEDFLRSEGVEYEEVDVPSFVADALEEGKLVGLFQGRMEFGPRALGNRSILADPRDDSVKERLNVALRRDVFQPFAPSMLWERAEEYLEDLGGRPNEFMTMSYTASEEFRKLAPAVVHVDGTTRPQAVREEINPTYYRIIKEFDKKTGIGAVLNTSFNMHGEPIVCSPADALKTFRNAKLDVLVVGGFAVFARGLPGQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8H6</accession>
    <name>A0A218P8H6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_07030</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854342.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8H6"/>
    <dbReference type="GeneID" id="33316009"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07030"/>
    <dbReference type="OrthoDB" id="43988at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06423">
        <property type="entry name" value="CESA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43630">
        <property type="entry name" value="POLY-BETA-1,6-N-ACETYL-D-GLUCOSAMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43630:SF1">
        <property type="entry name" value="POLY-BETA-1,6-N-ACETYL-D-GLUCOSAMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="273"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="320"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="4">
        <location>
            <begin position="40"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="349" mass="39790" checksum="27EED3606B7DD412" modified="2017-09-27" version="1">MNPLLLGLLAIFLWDGYFFFNYIISLFRNYRIREWEPKVSILIPAYNEGERILGAIKAALAQDYPDFEVIVIDDGSGDGTFEAASSVSDPRLKVYRKEHGGKARALNFGLSKASGEVIVTTDADSYLEEDALRELVRRFYSEEVLAVGGQVRVEGGSFLERAQDAEHLRIVMFRRAKELDDLSVAPGPIAAFRREAFERIGGFVEDVVEDYATTKVLKGFGKVVYAPSARVWTTMPRSLGALWRQRKRWFLGDLKNLGGGFTKDWAFLLLGDVIAFLDVLIPPILILTGLWEFFVLWWFFETFTMFLPTLFEGGRLSNALLFPMIVWFWAAFYLTLHIYGYVRLLLGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8H7</accession>
    <name>A0A218P8H7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Beta-galactosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_06980</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854333.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8H7"/>
    <dbReference type="GeneID" id="33315999"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06980"/>
    <dbReference type="OrthoDB" id="84443at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053427">
        <property type="entry name" value="Beta-galactosidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="GH_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018120">
        <property type="entry name" value="Glyco_hydro_1_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041004">
        <property type="entry name" value="Beta_gal_BgaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF209">
        <property type="entry name" value="GALACTOLIPID GALACTOSYLTRANSFERASE SFR2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00572">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="502" mass="57636" checksum="F48FFC2680DB5162" modified="2017-09-27" version="1">MKKEFLWGVSQSGFQFEMGDRFNRNLDTRSDWWHWVRDPVNLKKELVSGDLPEEGINNYELYPLDHSLARELGLNAYSLNLEWSRIFPCPTYGVEVDYELDGNGLIKRVKITKETLRELDEIANKNEVHHYTRVLANLKKLGFKVALTLTHYTHPVWLHDPIASRDTNLQNERNGWVSQKSVLEFAKFAAYLTYKFGHLVDIWATFNEPMVMVELGYLAPYSGFPPGVVNPQRAKEAIINIINAHARAYDAIREFKKEAPIGIIANNVGTSYPKDPNDPEDIKAAQMTDFFHSGLLLQALTKGDLNIEFDMETTIKVPHLKRLDWIGITYYSREVVTHSKPKFPEIPITGFKGVPGYGYSCPPNELSKDEHPVSDIGWEVYPEGIYNSIKAALEYGKPVYVMENGIADSKDLLRPYFIASHVAYIERALESGFDVRGYFHWALTDNYEWAMGFRMRFGLYSVDMITKERIPRKESVGVYREIVENGGLTERIRREYLGGEGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8I2</accession>
    <name>A0A218P8I2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4097 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8I2"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07110"/>
    <dbReference type="OrthoDB" id="101349at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025164">
        <property type="entry name" value="Toastrack_DUF4097"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13349">
        <property type="entry name" value="DUF4097"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4097" evidence="1">
        <location>
            <begin position="83"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="217" mass="23612" checksum="98EE4261D4763B78" modified="2017-09-27" version="1">MIFENVKEVEIKAVNGKLNIEGWEQSHVEVDYTRHGEVDVEIKKRGDKLIIKEEPKKRFLNLFGEGGWAEINLKVPKAVVVNAKNVNGELKAKDVRFGEVTTVNGRISLEDCEAEKLSTVNGRIRANLKVAGPLKASTVNGNIEITLDDLEGDVEVSTVNGGVALYLTDFCDARITAKAVNGGVHFVGIDPENPVIGTGDYEVKVSTVNGSVRVELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8J1</accession>
    <name>A0A218P8J1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_07005</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854338.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8J1"/>
    <dbReference type="SMR" id="A0A218P8J1"/>
    <dbReference type="GeneID" id="33316004"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07005"/>
    <dbReference type="OrthoDB" id="7442at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42901">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42901:SF1">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="228" mass="25285" checksum="1026B0D46CDB986B" modified="2017-09-27" version="1">MKVAVVTGASKGIGRAIAEALAREGYNLALGARSVDLLEGLSRELEEKHGVKVLHRYLDVSSPQSLKEFAEAVLEEFRGVDLLVVNAGIPMGGRLDEVSDEDMERVFQVNALGVWRTIKLFLPSLKERRGTVAVVTSYISTMILANAGAYVASKWAARAFTKTFQVENPEVKFIELRPGMVDTYFYGKPGRKIEEGFMKPEDVAELLVALLKLPKHVLVEEVLFRSIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8K0</accession>
    <name>A0A218P8K0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CopG family transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06985</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854334.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8K0"/>
    <dbReference type="GeneID" id="33316000"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06985"/>
    <dbReference type="OrthoDB" id="101914at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="69" mass="8127" checksum="62C7315ED51B354A" modified="2017-09-27" version="1">MKTIAVDEETWNAIKKLKAKLDARSYDEVLKILIETWHSTNLDKKLREISLDEEESELALEILKKLKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8K4</accession>
    <name>A0A218P8K4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">MFS transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_07115</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854357.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8K4"/>
    <dbReference type="GeneID" id="33316026"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07115"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06173">
        <property type="entry name" value="MFS_MefA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022324">
        <property type="entry name" value="Bacilysin_exporter_BacE_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43266">
        <property type="entry name" value="MACROLIDE-EFFLUX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43266:SF9">
        <property type="entry name" value="PERMEASE, MAJOR FACILITATOR SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01988">
        <property type="entry name" value="EXPORTERBACE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="212"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="304"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="347"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="376"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="213"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="420" mass="46058" checksum="EA5D34A48963E9D4" modified="2017-09-27" version="1">MRFNRDFWLFAVGRFVSQLGWAVQDVALPLYVLDQTRSGSMMSLFIIAEILPRALLSPIAGVVGDRYNRKNLMVWLDILRGLLLFAVIGLDMLGIRELLVVQVAMSIMGTFFGAATGAMYPDLVREEELASANSLLQSFGVIARIAGPALGGIIYAFGGIRLAILINAASFFGSGLFEAFIHYEWTSRKIESLGEVVDDLREGIAFLRSNKYLTVILSYALFINFLLNPVFAVALPYVYRVELAFSSQWFGFLQTAFMVGMLLGNVAIMAKLGSRAEGMLFRALFVQLGLVLVLAILISPLLSMSALTLFALFMGLNAVDGFFNALVNVPIVTKLQKAIPSELRGRIFSVFDLIVGMTIPAGMALVGFALDYVPAWVLFAVSGFLGIAVTAYYYLRYGSLLEGHLEAEVTRSEPEVPEIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8L9</accession>
    <name>A0A218P8L9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0235 protein A3L08_07150</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_07150</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0235 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854364.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8L9"/>
    <dbReference type="GeneID" id="33316033"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07150"/>
    <dbReference type="OrthoDB" id="53248at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1200.10">
        <property type="entry name" value="YggU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00634">
        <property type="entry name" value="UPF0235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003746">
        <property type="entry name" value="DUF167"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036591">
        <property type="entry name" value="YggU-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00251">
        <property type="entry name" value="DUF167 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13420">
        <property type="entry name" value="UPF0235 PROTEIN C15ORF40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13420:SF7">
        <property type="entry name" value="UPF0235 PROTEIN C15ORF40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02594">
        <property type="entry name" value="DUF167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01152">
        <property type="entry name" value="DUF167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69786">
        <property type="entry name" value="YggU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="95" mass="10655" checksum="5892574EB00344B3" modified="2017-09-27" version="1">MAKFIKETKEGTLLFIHVQPKAKKNAIEGIDEWRGRLKVKVKAPPVEGKANKEVVKFFSKLLGAEVEIVKGETSREKDLLVKGLKAEDVRKKLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8P0</accession>
    <name>A0A218P8P0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zn-dependent hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854412.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8P0"/>
    <dbReference type="GeneID" id="33316087"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07420"/>
    <dbReference type="OrthoDB" id="197151at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050855">
        <property type="entry name" value="NDM-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951:SF14">
        <property type="entry name" value="METALLO-BETA-LACTAMASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="293" mass="32501" checksum="BA94261CABB9F338" modified="2017-09-27" version="1">MALKKLGDSVYLYPGSPSTLIRVLDGGEAVLIDPGHGKGRHKDLKREIRRLELEVKAQLATHGHADHIAVAPKISAPLFIHRFEFSVAESPLNRELLTFGSKAPKGFLVFQFPEEVKVHAVFEWGDGQFGLNAIKLNGHSPGMTGFLDEENGLIYAGDSFFGEKLIQSVGLPYLIDPELFKASLNELHNYARRGYLLIPSHGKPVGGEEATELLEFNLRRVEETESLILGLLEEPASMDELAFRIMEHYGVAITPQKLALNLVPVRAFIAELYNRGEIEAVVDRGLKWAVKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8P4</accession>
    <name>A0A218P8P4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GTP cyclohydrolase MptA</fullName>
            <ecNumber evidence="4">3.5.4.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">GTP cyclohydrolase IV</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mptA</name>
        <name evidence="5" type="ORF">A3L08_07365</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Converts GTP to 7,8-dihydro-D-neopterin 2',3'-cyclic phosphate, the first intermediate in the biosynthesis of coenzyme methanopterin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>GTP + H2O = 7,8-dihydroneopterin 2',3'-cyclic phosphate + formate + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25860"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58854"/>
            <dbReference type="EC" id="3.5.4.39"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="4">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GTP cyclohydrolase IV family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.39" evidence="4"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854405.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8P4"/>
    <dbReference type="GeneID" id="33316076"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07365"/>
    <dbReference type="OrthoDB" id="53087at2157"/>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003934">
        <property type="term" value="F:GTP cyclohydrolase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044682">
        <property type="term" value="F:GTP cyclohydrolase IV activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001118">
        <property type="term" value="P:tetrahydromethanopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.270.10">
        <property type="entry name" value="Urate Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01527_A">
        <property type="entry name" value="GTP_cyclohydrol_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003801">
        <property type="entry name" value="GTP_cyclohydrolase_FolE2/MptA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022840">
        <property type="entry name" value="GTP_cyclohydrolase_MptA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010204">
        <property type="entry name" value="PRK13675.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36445">
        <property type="entry name" value="GTP CYCLOHYDROLASE MPTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36445:SF1">
        <property type="entry name" value="GTP CYCLOHYDROLASE MPTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02649">
        <property type="entry name" value="GCHY-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="site" description="May be catalytically important" evidence="4">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="268" mass="30609" checksum="B2794B228E03D54C" modified="2017-09-27" version="1">MPIFETQEETPKIRERLHRVGITNLRTVAKINWKGKVYTFLPIFEITIDVPEEKKGIHMSRLVESITEAMSEAVEEEVLEAHTSLEELGKCIIKRLEGKHPHKRAEVWIKTQLVIPRETPASKRLSYEPYDVEVGVIKNYDGSFEKVLRVKVIGNTACPHAMANNNGKTHIQRAVGELEVRAPFNEEIPLEDMIDVVESSFSHPTYTLLKTVDENAVVQGMHANPKFVEDVAREVFSKAKERFKGKIHVRVISNESIHKHDVIAETWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Q0</accession>
    <name>A0A218P8Q0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF530 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_07345</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854401.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Q0"/>
    <dbReference type="GeneID" id="33316072"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07345"/>
    <dbReference type="OrthoDB" id="85577at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007503">
        <property type="entry name" value="DUF530"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014496">
        <property type="entry name" value="UCP018863"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04409">
        <property type="entry name" value="DUF530"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018863">
        <property type="entry name" value="UCP018863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="415" mass="47691" checksum="5E2CD283CC1F5118" modified="2017-09-27" version="1">MTTTEELVAQVNKILDDIGIDMDGLFETFDIPSISLRLKENLSLLQELEEDLSKRVGEATPSADFSDRKNRDPHIQWIYKKKRNRILALERLRSAITAHKMALALISANYTFFLGKKLITIKELTRENAENVKAVSNPIQLGRVEVLPYLAYSGDVLRLLSRESIAVRESFKLIKGKLREKGTVRTRGLRIEVEYWENNRLKKARLDLPTDADIEAELRQRYGRRFRWRVLSFVKTKGVLINNHYTVDNLALAYSILDPEDGAKKLGLDLFRYYFLTSENDRESLGLYPDIRLCIDCHYSIFDLPFQDEPGFKTGHGSMLLMRKCEMEKALVGRRKDIANIPNHLLGGVLLYGMSDYSENRVAELLGIPEDELVEAIKKFVISGLHKTLFADTKKFEKFMPKSDRAKQFLALLQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8R3</accession>
    <name>A0A218P8R3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cell wall-binding repeat 2 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_07465</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854420.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8R3"/>
    <dbReference type="GeneID" id="33316096"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07465"/>
    <dbReference type="OrthoDB" id="86217at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12090">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="393" mass="43870" checksum="9DD7B94AE3C8AEFE" modified="2017-09-27" version="1">MMWKKGVALLFGLMLITAGLAFGKAAAAESSVTVILVSDNEADSALAEYLANITGAFVVTTTWGIYDPNVTAEIMNYAPDQVIIIGGPDAVVDQYVGDLENLNITVERWWDANRYETNVAVIGNATVKLKLKFENSVIVVPGNDTGAIKEALRRAVKVHGIILLANNTTDPVRVMAKFQIRPKNMTIIRSHVTKGVAERVREKVANQTNITEVEVNITPEMALQAINISEQRIATAEEMLANVTLPPQIERVSEKMLELARKELNISKEAYGEGNYGKAYGQAIAAKAHAEFVIRMASKEWQNKIRFDPAKRAELFVHRVETQLRIMEKAGVNVTEIRELVDQLKTAIENKDYDTIDALMERIQRKLLETYTRGKDKFRDHIMLPKRGKHGEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8S2</accession>
    <name>A0A218P8S2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">2-phosphoglycerate kinase</fullName>
            <shortName evidence="5">2PGK</shortName>
            <ecNumber evidence="5">2.7.2.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pgk2</name>
        <name evidence="8" type="ORF">A3L08_07435</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the phosphorylation of 2-phosphoglycerate to 2,3-diphosphoglycerate. Involved in the biosynthesis of cyclic 2,3-bisphosphoglycerate, a thermoprotectant.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(2R)-2-phosphoglycerate + ATP = (2R)-2,3-bisphosphoglycerate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42408"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58248"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Thermoadapter biosynthesis; cyclic 2,3-diphosphoglycerate biosynthesis; cyclic 2,3-diphosphoglycerate from 2-phospho-D-glycerate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 2-phosphoglycerate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.16" evidence="5"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854415.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8S2"/>
    <dbReference type="GeneID" id="33316090"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07435"/>
    <dbReference type="OrthoDB" id="358692at2157"/>
    <dbReference type="UniPathway" id="UPA00551">
        <property type="reaction ID" value="UER00609"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016774">
        <property type="term" value="F:phosphotransferase activity, carboxyl group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00769">
        <property type="entry name" value="2PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020872">
        <property type="entry name" value="2PKG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003259">
        <property type="entry name" value="PRK04220.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33477">
        <property type="entry name" value="P-LOOP NTPASE DOMAIN-CONTAINING PROTEIN LPA1 HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33477:SF3">
        <property type="entry name" value="P-LOOP NTPASE DOMAIN-CONTAINING PROTEIN LPA1 HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-cone" evidence="7">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="292" mass="33082" checksum="3912CC92328E4E9E" modified="2017-09-27" version="1">MIVVTDSERKIRLPFSRGILTRSITLAGIDVGVAYAIATEVQKELGRRKRYAVTTDEIRELTYSKLIEKGLKDAAERYLFWRTLRRRRVRLAVLLGGTTGVGKSTIATELAFRLGIRSIIGTDTIREVLRKVIARELLPDIHVSSFLASEVVSVPKGMNPLVYGFETQVKHVSVGIRAVLERSRREGLNTLIEGIHVVPGFVETTGNEFMYLITVPSEEHLISHFYERARYSARGVERYIENADRIMAIQEYLVERAREFGVPVIENVELESTVSAIMEDMMKKLKGKVEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P944</accession>
    <name>A0A218P944_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DsrE family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_08170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P944"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08170"/>
    <dbReference type="OrthoDB" id="41780at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="12379" checksum="0827129333C220FE" modified="2017-09-27" version="1">MVKALVIISSSDDKALVGFMWSTNALKYKWVEDVEVILFGPIEKAVAEGDERFLPWIEKLKELGKLPIACKRIAELEGFEVSLEKHAKVEYVGKIIAEYLEKGYVPMTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P953</accession>
    <name>A0A218P953_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycosyl transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_08205</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854549.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P953"/>
    <dbReference type="GeneID" id="33316247"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08205"/>
    <dbReference type="OrthoDB" id="132546at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12526:SF510">
        <property type="entry name" value="D-INOSITOL 3-PHOSPHATE GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12526">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="4">
        <location>
            <begin position="22"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="3">
        <location>
            <begin position="203"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="400" mass="45286" checksum="ADFAE6D4DD817812" modified="2017-09-27" version="1">MKVAMVTPHGDPLGKLGEPDTGGQCVYVKELSRHLGALGVKVDIFTRQRGGRKEIEYINEKVRVIRIECGPEGFIPKEKLMPYLPEFTDKVSEYFEKEGYDIVHTHYWDGGFVGMELKERHGVKMVHTSHSLGILKAKALGDFEPYRERIDLEKKIYETSDAIVATTEIEKRDIASLYEIDEGKIHVIPIGVDTTFYRPMGNKKELKRELGLPEVPLVFALARLDPRKGLDLLIKSVPYVREYYQGDFLVLISTGTGAKEEEKEMNKLLSLIENLGVTGYVKIIPAIEPVSMVPKYYSAADVFVLPSPYEPFGIVMLEAMACKTPIVATKFGGPAEVLQDGYDGFLVDPKDSKEMGKKIAFLLEDEELRKVFAERAYRKVTEKYSWDSVAREMRNLYEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P956</accession>
    <name>A0A218P956_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MIP18 family-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854560.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P956"/>
    <dbReference type="GeneID" id="33316258"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08260"/>
    <dbReference type="OrthoDB" id="85378at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIP18 family-like" evidence="1">
        <location>
            <begin position="17"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="119" mass="13515" checksum="7FFC6ECC2004D6CA" modified="2017-09-27" version="1">MKVYMPDREWPEHYKPVLEELAKITDPVTGGDILDSGVIAGLEVSDDTLKVWLKFESHAEYNITGESAIAYSKIIGDIMERFALVKFQNVYVYDLKNNPVGVFENKKGYTIEDISPERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P963</accession>
    <name>A0A218P963_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutaredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_08230</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854554.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P963"/>
    <dbReference type="GeneID" id="33316252"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08230"/>
    <dbReference type="OrthoDB" id="35385at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02973">
        <property type="entry name" value="TRX_GRX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13192">
        <property type="entry name" value="Thioredoxin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin-like fold" evidence="3">
        <location>
            <begin position="33"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="119" mass="13664" checksum="F1A572A217931995" modified="2017-09-27" version="1">MDELEMIRRKKMLELMKKAGMIEVKPREPKVVIEVITSPGCPYCPIAWAMAQELERKYEGVVARELSVATPEGRRKAIEHNIMGTPTILINNRVEFIGVPNFAEFERRVRELLSASRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P990</accession>
    <name>A0A218P990_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Adenylosuccinate synthetase</fullName>
            <shortName evidence="8">AMPSase</shortName>
            <shortName evidence="8">AdSS</shortName>
            <ecNumber evidence="8 9">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">purA</name>
        <name evidence="10" type="ORF">A3L08_08460</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>IMP + L-aspartate + GTP = N(6)-(1,2-dicarboxyethyl)-AMP + GDP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8 9">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="8 9"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854598.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P990"/>
    <dbReference type="GeneID" id="33316298"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08460"/>
    <dbReference type="OrthoDB" id="372247at2157"/>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00335"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046040">
        <property type="term" value="P:IMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03108">
        <property type="entry name" value="AdSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.440.10:FF:000005">
        <property type="entry name" value="Adenylosuccinate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.440.10:FF:000007">
        <property type="entry name" value="Adenylosuccinate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.170.10:FF:000002">
        <property type="entry name" value="Adenylosuccinate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.440.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.300.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.170.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00011">
        <property type="entry name" value="Adenylosucc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018220">
        <property type="entry name" value="Adenylosuccin_syn_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042109">
        <property type="entry name" value="Adenylosuccinate_synth_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042110">
        <property type="entry name" value="Adenylosuccinate_synth_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042111">
        <property type="entry name" value="Adenylosuccinate_synth_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001114">
        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003295">
        <property type="entry name" value="PRK04293.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846:SF0">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00788">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01266">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 8 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="12"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <begin position="13"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <begin position="40"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="42"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="252"/>
            <end position="258"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="284"/>
            <end position="286"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="324"/>
            <end position="326"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ07351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="339" mass="37306" checksum="35D647040B71FBDF" modified="2017-09-27" version="1">MPSYIVVGGQWGDEGKGSVIAYLALKDKPDVIARGGVGTNAGHSVFIDGRKYAVRQLPTGFMQTKARLLVGAGVLVDPEVFFHELEHLQEFKVAERVGIDYRCAIIEEKHKQLDRTNGHLHEKIGTTGSGCGPANADRVMRRAKLAKDIPELEPYLTDVAAEVNDALDEGKLVLVEGTQGFGLSLYYGTYPYVTSKDTTASAIASDVGIGPTRVDDVIVVFKSFPTRVGAGPFPTEMSEEEAERMGLIEYGTVTGRRRRVGWFDFEFARYSARINGATMLALTMLDKYDKGAFGVTDYDKLPKKAKEFVEEIEERTGVPVALIKTGPELEHIIDRRENI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P991</accession>
    <name>A0A218P991_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cobalamin biosynthesis protein CobQ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854602.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P991"/>
    <dbReference type="GeneID" id="33316302"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08480"/>
    <dbReference type="OrthoDB" id="65817at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43063">
        <property type="entry name" value="4FE-4S CLUSTER CONTAINING PARA FAMILY ATPASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43063:SF1">
        <property type="entry name" value="4FE-4S CLUSTER CONTAINING PARA FAMILY ATPASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="61"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="93"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="290" mass="31398" checksum="E830699FB3E7C84E" modified="2017-09-27" version="1">MQIAVSGGKGGTGKSTVAVNLAIALKQLGTELTFADLDVEAPNDHLLLGVELANEEPVVQFMPRFDYSKCTKCRKCAEVCEEHAIITLKDGTPFLMPNLCSGCRACEIVCPVPGAILEGQRLIGHTYITPTPYGFTLVTGKLREGEERSMPLVVAAKKKALEVRKGLLFIDTAAGTGNTVSKAVEFSDLLIAVTEPTPLGIHDTELILELGKLMEMPTWVVINRADLGEKEKVYELAKKYGAEVVAEIPYSENIVKSYVEGKPIVLTNYPEAEIFEGLAERVLKFIGGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9A2</accession>
    <name>A0A218P9A2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_08550</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854612.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9A2"/>
    <dbReference type="GeneID" id="33316315"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08550"/>
    <dbReference type="OrthoDB" id="95391at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="189" mass="21757" checksum="7C7BF3FF991B23C4" modified="2017-09-27" version="1">MRVKEAIMAILPELEELGEVEFGQYSPPYPNLLFAFLGSGKRGLPEFERFAEKTVGKDAVGQILLSLLQYLLIRYRRYGEYSVVKPTIKVFLTLNGWLNEKGFESEWKLLLHNFIGYLVDMAAKIEEREDCETALSYLTVVYRLTKEASEDFTEEYFRKLSETVGEKLDSLRESCGEIGHKFKKDAQGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9B1</accession>
    <name>A0A218P9B1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854622.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9B1"/>
    <dbReference type="GeneID" id="33316330"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08620"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="65" mass="8292" checksum="7B47E22CA62F5DC5" modified="2017-09-27" version="1">MYYPPKKKVQPRKVEEEEEEFEPLDAVEEAYEDWDEEEEWDEEEEWDNGDWDEEEEWDEDWDDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9G3</accession>
    <name>A0A218P9G3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7343 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_08825</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854660.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9G3"/>
    <dbReference type="GeneID" id="33316371"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08825"/>
    <dbReference type="OrthoDB" id="109251at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055767">
        <property type="entry name" value="DUF7343"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24034">
        <property type="entry name" value="DUF7343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="223"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7343" evidence="2">
        <location>
            <begin position="258"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="321" mass="36115" checksum="A9D42DE3BB244745" modified="2017-09-27" version="1">MSAVENKEPRGRKPALIGLVLPFLLLSLFLPMVHAQAYDYEINAYAVYFDVITPDNIRETVEMDLTSRVNLTRYVIYTDYPVENPKAVMERGNTTVPVNVTVGDVLGGTNAVYMNFPLLRPGESAKIRLTFRTRGMITETDGKQQFTYYVKFSQPVGLLHVQLVVPKGYAVLSPIIPSPDRVESSTTRLLLEWDRQNVRAGEEFYFIVGFSGEITPPKQPSPLFYAGLFMAGLVIGGGTVYGYILYRERKRSGELTHLRTDEEKVLAILKEGPVLQSELAEKLGVSKAKVSILLREMEEKGLIVRVKEGRTYRVELATRET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9H8</accession>
    <name>A0A218P9H8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cobalt ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_08935</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854680.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9H8"/>
    <dbReference type="GeneID" id="33316393"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08935"/>
    <dbReference type="OrthoDB" id="31170at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16914">
        <property type="entry name" value="EcfT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003339">
        <property type="entry name" value="ABC/ECF_trnsptr_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33514">
        <property type="entry name" value="PROTEIN ABCI12, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33514:SF13">
        <property type="entry name" value="PROTEIN ABCI12, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02361">
        <property type="entry name" value="CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="253" mass="28674" checksum="B4CDF97ECB6F29CC" modified="2017-09-27" version="1">MIYLFYSERDSLLHSLDPRVKIIGTAAGIAAIMLYNDPKILIPLFFVFLVIGRFLGKVGIGEQLRLLKPLLPIVIITIVVWPFIYRPRLMGLLFGVSFAMRLLTFALITFLLLMTTSQRDLILGFVRLGMPYEFGLTISIALRYIPTLYILSRNIMDAQRSRGWEVEKGNFIVRARKMTAVLIPLLVASLKTAHELSIALESRALGASEKRTFLYDIRMGRKDYAAVVLILALFALTIYVRYGLGIGHVRIYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9H9</accession>
    <name>A0A218P9H9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF447 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854689.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9H9"/>
    <dbReference type="GeneID" id="33316404"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08990"/>
    <dbReference type="OrthoDB" id="146030at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.290">
        <property type="entry name" value="Hypothetical membrane protein ta0354_69_121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007386">
        <property type="entry name" value="DUF447_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016733">
        <property type="entry name" value="UCP018747"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04289">
        <property type="entry name" value="DUF447_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018747">
        <property type="entry name" value="UCP018747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF447" evidence="1">
        <location>
            <begin position="13"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="198" mass="22415" checksum="E9E6479A25F17B24" modified="2017-09-27" version="1">MELLELLNEGQVYEVLLVTESNVTPVGVVRRGHSLFFKLFGGKSAVEIKRRPLASIQITNDVELLVRLALNLEITLEFEEKDGHRWIKGLPGVYGRVEFGEEHHEDELGSTVVLKCSLVPEGEIRGNQPPRPVSRADNYLLEMAVNLTRLLVAVRNGKREEAERLYLEVVRDYDMYKKFGGGSETAERMLEIAMGTFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9K0</accession>
    <name>A0A218P9K0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854715.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9K0"/>
    <dbReference type="GeneID" id="33316434"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09140"/>
    <dbReference type="OrthoDB" id="102580at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="21"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="109" mass="13119" checksum="4CBDD2CA126CEEA8" modified="2017-09-27" version="1">MKIELIIPRERFKAIKGKDINALLRENLPKAEETLKADREEFLCEKIGKLEEKLREMESEIEELREFYEKALRDKELMMAERDRLRKENAELRAKFEEKKSGLEKVHKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9K4</accession>
    <name>A0A218P9K4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0179 protein A3L08_09105</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_09105</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0179 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854709.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9K4"/>
    <dbReference type="GeneID" id="33316427"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09105"/>
    <dbReference type="OrthoDB" id="24613at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00498">
        <property type="entry name" value="UPF0179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005369">
        <property type="entry name" value="UPF0179"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002253">
        <property type="entry name" value="PRK01177.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40699">
        <property type="entry name" value="UPF0179 PROTEIN MJ1627"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40699:SF1">
        <property type="entry name" value="UPF0179 PROTEIN MJ1627"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03684">
        <property type="entry name" value="UPF0179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006595">
        <property type="entry name" value="UCP006595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="144" mass="15839" checksum="E43FB4E7AAE2E1D1" modified="2017-09-27" version="1">MAIITLVGEKLARPGVEFIFYGPAEPCKTCKLAGVCVGNLEPGRRYKILRVRSMPSHSCPLHEGKVRVVEVVEPSIEVAIEPRLAIAGSVIKLQFADCSDKEKADLFRPEGLLEGDQVKIIEVTGEVECDGKTYKVAKVMRKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9K5</accession>
    <name>A0A218P9K5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Asparagine--tRNA ligase</fullName>
            <ecNumber evidence="2 8">6.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_08850</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.22" evidence="2 8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854665.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9K5"/>
    <dbReference type="GeneID" id="33316376"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08850"/>
    <dbReference type="OrthoDB" id="5908at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006421">
        <property type="term" value="P:asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04319">
        <property type="entry name" value="PhAsnRS_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004522">
        <property type="entry name" value="Asn-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00457">
        <property type="entry name" value="asnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003037">
        <property type="entry name" value="PRK03932.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003483">
        <property type="entry name" value="PRK05159.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF34">
        <property type="entry name" value="ASPARAGINE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="9">
        <location>
            <begin position="131"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="NCBIfam" id="TIGR00457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ07420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="431" mass="49688" checksum="57C185576DD21C0A" modified="2017-09-27" version="1">MIDKVYCADVTPEMEGKRVKLAGWVYRKREVGKKVFIVLRDSSGIVQTVFKKELSEEAYAEAKKVGIESSVIIEGTVKADPRAPTGVEVQADRIEVIQNVDFFPITKDASEEFLLDVRHLHLHSPKVAAVMKVKATLMQAAREWLTQDGWYEVFPPILVTGAVEGGATLFKLKYFDKTAYLSQSAQLYLEAAIFGLEKVWSLTPSFRAEKSRTRRHLTEFWHLELEAAWMDLWDIMKVEEELVSYMVQRTLELRKKEIELYRKDDIKTLKNAVPPFPRISYDEAIDILQSKGVQIEWGEDMGADEERILTQEFEAPFFVYGYPKHIKAFYMKEDPSDPRKVLAADMLAPEGYGEIIGGSQREDSYDKLVQRILEEGMKPEDYEWYLDLRKYGSVPHSGFGLGLERLVAWVLKLDHVRWATLFPRTPSRLYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9L8</accession>
    <name>A0A218P9L8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_08970</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854685.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9L8"/>
    <dbReference type="GeneID" id="33316400"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08970"/>
    <dbReference type="OrthoDB" id="18576at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027552">
        <property type="entry name" value="CGP_CTERM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04288">
        <property type="entry name" value="CGP_CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="351" mass="37359" checksum="CEBE4269BE194CE9" modified="2017-09-27" version="1">MKKAAIILAVFVLFGVFGFAAASATTIGVDLAHGENDKGLTVLTDKDGNVVADGMIKTISDVTWAYIGPADKADTLGIQQVGEQITYDAIKDIDFLIIGQPAQAFTPDEINALKQWWDDGNRILWIAGDSDYGSGVQTIDNVNAVLDALGVNLRLDQCSVEDSQSNAGAGYRVVGIVNPDDKTPNKEDITKDLQNGGKVLFHGPGVVAYVDDSGNWQTIQPNAGLENVYVIVTSSENGQIVENSDPAANAYTVGDTGKFPLLAVQVFPDKKNVLIVSGETPYGGYEPMWAPEYSEVKLDGPTFVTNFIHWAVSTQQSLGEEEGSEGSGSTCGPAALVGLALIPLALYRKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9N9</accession>
    <name>A0A218P9N9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF998 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854740.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9N9"/>
    <dbReference type="GeneID" id="33316464"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09290"/>
    <dbReference type="OrthoDB" id="103507at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009339">
        <property type="entry name" value="DUF998"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42241">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED, DUF998 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42241:SF2">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED, DUF998 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06197">
        <property type="entry name" value="DUF998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="20437" checksum="F1A53795C313659A" modified="2017-09-27" version="1">MEFEKLSAYISVFLPVIFLAGLTIVLSKNPWFSFTDNALSDMGSLKNPERWYFNGFLMTFAVITFIAAFGAFRSGLSYLMIFASVSLFLVGVFPEELPYHSPSAVLFYVLALADIALVGIKLGRSGISFGYLWSVLALLTFFLMFYLVNARVFKGLAIPEMIGAITILTWFVYIGLLRLCSQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9S7</accession>
    <name>A0A218P9S7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_09525</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854784.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9S7"/>
    <dbReference type="GeneID" id="33316511"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09525"/>
    <dbReference type="OrthoDB" id="87732at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43335">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43335:SF4">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="6"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="312" mass="35548" checksum="6EF3C7A57F116380" modified="2017-09-27" version="1">METYVIETRDLTKFFGKRNVVYHLNLKVPKGVVYGFLGPNGAGKTTTIKMLTGALKPTYGEIRIFGMEMPRERVEIMRKAGYMPEVPIAYEDMTIFEFLVYMGRLSGMKKGDAVEQAKRLMQYTGVGRLALNKIKELSSGQKQRVSFAAALMGDPELLILDEPTANLDPLGRIEFIGKVLELARQGKSIFISSHIVSEVEKMCNHVGIINRGVLVAQGRIRDLVSVEEDEYDVVTSDNDKAIAFLREKPYVREVWEEEGVIRVRVEPRFLEEFFLAFPRYLVENGVRLKLFRPHTSPLERILMEKFGMGEET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9T0</accession>
    <name>A0A218P9T0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09585</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854795.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9T0"/>
    <dbReference type="GeneID" id="33316523"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09585"/>
    <dbReference type="OrthoDB" id="53037at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42663:SF12">
        <property type="entry name" value="ATP-BINDING PROTEIN PHNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42663">
        <property type="entry name" value="HYDROLASE C777.06C-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="37"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="249" mass="28212" checksum="3DB55AA43E0730C0" modified="2017-09-27" version="1">MLVYFIGTGGSEGIPAHLCTCSTCNEARKFGFAQRRPSTLAIITENKRAVLFDVGTDIRDFLNVPLEAIFLTHWHHDHIYGLYKLRWMAKETRLYAPEGHADALILNDPKNLRPNVIKPGQTLKVDTLKITPIRLNHQVETLGYLIEEDGKSVAILYDTKGLPEGTREFLEERAPLRLAIVDATYPPGTDDPYHNNVDEAAEIGLSLAERTVLSHISHKNLPFLELTEYVRKRWGNRVLVAYDGMVFYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9T8</accession>
    <name>A0A218P9T8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09305</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854743.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9T8"/>
    <dbReference type="GeneID" id="33316467"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09305"/>
    <dbReference type="OrthoDB" id="86311at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="122" mass="13728" checksum="37C20223C24BDE9B" modified="2017-09-27" version="1">MKRLGLATLLLSINGLLLLYYAYAWSSLVYLAFALFSLLLAYGVGRENRTAVKVALIYAGMAFFFGLLFLIAGNLYSAVDAAISFFIMHDILGYIQEVYREETAREKKTNGEEKIEKPPESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9U5</accession>
    <name>A0A218P9U5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09665</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854810.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9U5"/>
    <dbReference type="GeneID" id="33316539"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09665"/>
    <dbReference type="OrthoDB" id="131084at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="90" mass="11047" checksum="A53EF0D01D0B6725" modified="2017-09-27" version="1">MSEVVIPKRDVLIYERLRIISELAPIRERVKAFEKKYGMTLKEFERKLMDSEESFEAWDDYIEWKAYVRKLEELEKRLKEIEHAERVRIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9U8</accession>
    <name>A0A218P9U8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Heat-shock protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_09535</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854786.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9U8"/>
    <dbReference type="GeneID" id="33316513"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09535"/>
    <dbReference type="OrthoDB" id="198277at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000072">
        <property type="entry name" value="Small heat shock protein HSP16.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="47"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="CS" evidence="4">
        <location>
            <begin position="52"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="162" mass="19714" checksum="1652F5FAB2CBF864" modified="2017-09-27" version="1">MAWRRDRYWDPFDLMREIQEEIDAIFRDVMRGPRLWGWRESEERTEFASETWREPFVDIFDRGDKFVITVELPGVRKEDIKLRVTEDTVYIEAQMKREKELEREGAIRIERYYSGYRRVIRLPEEVIPEKAKARYNNGVLEIEIPKKKPTKPEKEGFEVKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9X2</accession>
    <name>A0A218P9X2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09825</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854840.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9X2"/>
    <dbReference type="GeneID" id="33316571"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09825"/>
    <dbReference type="OrthoDB" id="97223at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="95" mass="10853" checksum="DCC77B5AA53E00E1" modified="2017-09-27" version="1">MERLKALMGKKGNRLEFTADLVDLLLTDRELYSDEVLFRDAVEEIYSTLRSEALENGRKDLVEAYENAVLLRAVVTDRVKGVEELLLEIKKNLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9X7</accession>
    <name>A0A218P9X7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleotidyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854794.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9X7"/>
    <dbReference type="GeneID" id="33316522"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09580"/>
    <dbReference type="OrthoDB" id="40412at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05403">
        <property type="entry name" value="NT_KNTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37030">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37030:SF1">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="32"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="122" mass="14339" checksum="EEEEF6728C8392A7" modified="2017-09-27" version="1">MSSREAVISRMIEDGRKRYLMIKHYRRYLPAIKRACEEVFGQCELYVFGSVLTGKFTAGSDVDLLIKVRKAPKNLREKAELEVKIEELANLPYYHPFEFHIVDEEGFRRYVEVLKVNPVKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA11</accession>
    <name>A0A218PA11_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_00555</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854846.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA11"/>
    <dbReference type="GeneID" id="33314715"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00555"/>
    <dbReference type="OrthoDB" id="372018at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901605">
        <property type="term" value="P:alpha-amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000053">
        <property type="entry name" value="Aminotransferase, class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000054">
        <property type="entry name" value="Aminotransferase, class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050859">
        <property type="entry name" value="Class-I_PLP-dep_aminotransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790:SF19">
        <property type="entry name" value="KYNURENINE_ALPHA-AMINOADIPATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="8">
        <location>
            <begin position="44"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="418" mass="47615" checksum="5DB0662FA04A03C3" modified="2017-09-27" version="1">MEEKLMQKLGSGSLDFESYFSEKALAMKASEVRELLKLVETSDVISLAGGLPAPETFPVEKIKKIAQEVLTHHADKALQYGTTKGFTPLRLTLADWMEKRYGIPTSKVEIMMVAGSQQALDLIGRTFINPGDLVVMEGPTYLAAINAFKYYGPEFISIPMDHDGMRIDLLEEKLEELKRQGKRVKFVYTVSTFQNPMGVTMSVERRKRLVELAREYDFLIVEDSPYSELRYSGEPVPPIKHFDDEGRVIYLGTFSKILAPGFRLGWVAAHPHFIRKMEIAKQSIDLCANTLAQVIAWKYVEEGYLDEHIPKIIEFYKPRRDAMLEALEEYMPEGVEWTKPDGGMFIWVTLPEGIDTKLMMEKAVSKGVAYVPGEAFFVHRDVKNTMRLNFTYVPEEKIHEGIKRLAEVIDEEIKALKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA34</accession>
    <name>A0A218PA34_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">D-aminoacyl-tRNA deacylase</fullName>
            <ecNumber evidence="4">3.1.1.96</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">dtdA</name>
        <name evidence="5" type="ORF">A3L08_04795</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">D-aminoacyl-tRNA deacylase with broad substrate specificity. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a D-aminoacyl-tRNA + H2O = a tRNA + a D-alpha-amino acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13953"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:10124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59871"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:79333"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>glycyl-tRNA(Ala) + H2O = tRNA(Ala) + glycine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53744"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:13640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DtdA deacylase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.96" evidence="4"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854892.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA34"/>
    <dbReference type="GeneID" id="33315563"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04795"/>
    <dbReference type="OrthoDB" id="9863at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051499">
        <property type="term" value="F:D-aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106026">
        <property type="term" value="F:Gly-tRNA(Ala) deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019478">
        <property type="term" value="P:D-amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.50:FF:000001">
        <property type="entry name" value="D-aminoacyl-tRNA deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10700">
        <property type="entry name" value="AF0625-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.50">
        <property type="entry name" value="AF0625-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00562">
        <property type="entry name" value="Deacylase_DtdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018033">
        <property type="entry name" value="Deacylase_DtdA_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007508">
        <property type="entry name" value="DtdA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003074">
        <property type="entry name" value="PRK03995.1-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34667">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34667:SF1">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04414">
        <property type="entry name" value="tRNA_deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016210">
        <property type="entry name" value="UCP016210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142535">
        <property type="entry name" value="AF0625-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="272" mass="30472" checksum="F2AA911BDC6D084F" modified="2017-09-27" version="1">MKVIMTTKIDPASMNILGKLVENFGFKETERVFDGNPVYSRENTLILTTNDEMIYYDNLDAEIERQLGERPEIIVFASRHSSKQKLPALTTHVTGNWGEAVYGGKDGSLAVAHPAAMKLALLKMNELNDLGWTVCYEATHHGPSELDVPSLFIEIGSSEEEWRNERAGEIIAETIVHVLKNYEKAKFPVAIGIGGGHYAPKQTKRVLETDIAFSHIAPKYVHPVDRQLLLRAIEKTYGGVDAIYVDWKGSRGETRQMARSLAEELGLEFIRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA45</accession>
    <name>A0A218PA45_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RNA-free ribonuclease P</fullName>
            <shortName evidence="5">RNA-free RNase P</shortName>
            <ecNumber evidence="5">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Protein-only RNase P</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_04770</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">RNA-free RNase P that catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the HARP family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="5"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854891.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA45"/>
    <dbReference type="GeneID" id="33315558"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04770"/>
    <dbReference type="OrthoDB" id="95197at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18691">
        <property type="entry name" value="PIN_VapC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01078">
        <property type="entry name" value="RNA_free_RNase_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014856">
        <property type="entry name" value="RNA_free_RNase_P"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003342">
        <property type="entry name" value="PRK04358.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03875">
        <property type="entry name" value="RNA_lig_partner"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41173:SF1">
        <property type="entry name" value="RNA-FREE RIBONUCLEASE P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41173">
        <property type="entry name" value="UPF0278 PROTEIN TK1425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08745">
        <property type="entry name" value="PIN_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="199" mass="23113" checksum="A5DDEAA9E1B0D00C" modified="2017-09-27" version="1">MRFVLDTSIFVNPEIRGKFGDNPTEAMRTFLGYAERLFGRVEFYMPPGIYREVMHFVDEEELLPEIELYIIKKPPNVHDIKIPAFVVYELIDDIRRRIDKGLRVAEKAVRESVIETDNVDRIIQKLRRNYRKALREGIVDSKEDFELILLAKELDATIVSADVGILTWAQKMGIKWVDAANFKEVLEGLSERLTKGKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA49</accession>
    <name>A0A218PA49_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoribosylformylglycinamidine synthase subunit PurL</fullName>
            <shortName evidence="8">FGAM synthase</shortName>
            <ecNumber evidence="8">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Formylglycinamide ribonucleotide amidotransferase subunit II</fullName>
            <shortName evidence="8">FGAR amidotransferase II</shortName>
            <shortName evidence="8">FGAR-AT II</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Glutamine amidotransferase PurL</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Phosphoribosylformylglycinamidine synthase subunit II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">purL</name>
        <name evidence="12" type="ORF">A3L08_03275</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the FGAMS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854878.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA49"/>
    <dbReference type="GeneID" id="33315261"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03275"/>
    <dbReference type="OrthoDB" id="8251at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02203">
        <property type="entry name" value="PurL_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02204">
        <property type="entry name" value="PurL_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.10:FF:000004">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase subunit PurL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00420">
        <property type="entry name" value="PurL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010074">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041609">
        <property type="entry name" value="PurL_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01736">
        <property type="entry name" value="FGAM_synth_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002290">
        <property type="entry name" value="PRK01213.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43555">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43555:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18072">
        <property type="entry name" value="FGAR-AT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001587">
        <property type="entry name" value="FGAM_synthase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribosylformylglycinamidine synthase linker" evidence="11">
        <location>
            <begin position="4"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like N-terminal" evidence="9">
        <location>
            <begin position="57"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="10">
        <location>
            <begin position="184"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like N-terminal" evidence="9">
        <location>
            <begin position="415"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="10">
        <location>
            <begin position="551"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="77"/>
            <end position="80"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
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        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
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        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="296"/>
            <end position="298"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
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        <location>
            <position position="471"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="508"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="509"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="511"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ07633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="714" mass="78079" checksum="FA0233A15C4DFC6C" modified="2017-09-27" version="1">MFPHEEKLIRERLGREPNEVEWAMLEVMWSEHASYKSSRPWLRLLPTENEHVVIGPGEDAGIVKFDDDTWIAVGIESHNHPSAVEPYGGAATGVGGIVRDILCMGARPMALLDPIRFGPLEKERNRYLFEGVVKGIADYGNRIGVPTVGGETEFDESLDNYTLVNVACIGIMKPEHLVHSYVTEAGLKLVLVGNRTGRDGIHGVTFASEELSENAEEEDRSAVQIPDPFTEKLLIEATLEAVYTGKVKALKDLGGGGLTCASSEMAEKKGFGAVIYADRVPLREPGVRPAEVMISESQERMLFAVKPEDIEEIGRIFEKYGLEWAIVGEVIEEPRYIVYWKGEKVADLPVELLTDVPTIEWEMKPYNVEKDVETPDIDFREAFDLVWGSPNVLSKRWVWEQYDHEVQGRTAVKPGMDSAVLKINDEYGLAFVADGNPNHSYLNPYHGAMGAVAEVVRNLVSVGAEPLALVDNLNFASPERPEVYWSFAETVRGLADAAKAFGLAYVSGNVSFYNEIAGKPIKPTPVVAGLGKVKLGKIPSMGLEEGLLIGVVGVTRKELGGSELYARLGVEGGLAPRVNLDEEKANAEGILKAIEKGLVSSVHDVSKGGIAVALAEMSLAGGVGFEVDLSAVHVEENLSPLEVAFSESHGRYVVAFPEESLDELKALFRHFAVIGRAGGSGAVFRWNGKELLRKSISELREVHESLPRLLGEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA72</accession>
    <name>A0A218PA72_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_07690</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA72"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07690"/>
    <dbReference type="OrthoDB" id="99141at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36449:SF1">
        <property type="entry name" value="ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36449">
        <property type="entry name" value="ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-1277">Toxin-antitoxin system</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="21843" checksum="3A467EFE489B18F3" modified="2017-09-27" version="1">MESVNCGEGGEEQTNFAREEAWDSHRRCLSTVFVVLSENRLVSYFTLSPFIVSLKIRENMPEPKKELVMELRGRLERLLGMDIKYSSVPTILLGQFCLQNEYRGRGIGGELLAKVIKPYAVLYAARIGGVGLSLHAKKQVAKRFYLNPKKNPLASGFQAVSKGSTYELFYPFAAEAIAVRRALIERFKSKEFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA99</accession>
    <name>A0A218PA99_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Galactokinase</fullName>
            <ecNumber evidence="12">2.7.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">A3L08_08155</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>alpha-D-galactose + ATP = alpha-D-galactose 1-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13553"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28061"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58336"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GHMP kinase family. GalK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.6" evidence="12"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854926.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA99"/>
    <dbReference type="GeneID" id="33316237"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08155"/>
    <dbReference type="OrthoDB" id="116110at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004335">
        <property type="term" value="F:galactokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000126">
        <property type="entry name" value="Galactokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.890:FF:000001">
        <property type="entry name" value="Galactokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000705">
        <property type="entry name" value="Galactokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019741">
        <property type="entry name" value="Galactokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019539">
        <property type="entry name" value="GalKase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006206">
        <property type="entry name" value="Mevalonate/galactokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00131">
        <property type="entry name" value="gal_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003006">
        <property type="entry name" value="PRK03817.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10457:SF7">
        <property type="entry name" value="GALACTOKINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10457">
        <property type="entry name" value="MEVALONATE KINASE/GALACTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10509">
        <property type="entry name" value="GalKase_gal_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000530">
        <property type="entry name" value="Galactokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00473">
        <property type="entry name" value="GALCTOKINASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00106">
        <property type="entry name" value="GALACTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="6 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Galactokinase N-terminal" evidence="15">
        <location>
            <begin position="5"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="13">
        <location>
            <begin position="69"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="14">
        <location>
            <begin position="249"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="NCBIfam" id="TIGR00131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF10509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ASJ07683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="348" mass="38822" checksum="96C43DCF5131D0A1" modified="2017-09-27" version="1">MRVLSPGRVNLIGEHTDYTFGYVMPMAVNLYTVIEGEKAGDVKLYSEHFNEEKAFSLNELVREGSWMDYVKGVYWALFEEGYKPGGIHGRVGGNLPLGAGLSSSASFELAVVAFLNKAYNLNLSRLEMVLLAKKAENEFVGVPCGILDQFSIAFGKKGHVIFLDTDTLEYEYIPFPEDVSVLVFYTGVKRELASSAYAERKEVAEEALRLLGKRTSKEVSEEEVSRLPALYARYLGYIVRENRRVLEVRDALKEGDITTVGEILTRAHWDIAKNYGVSSDELDFFVRKANELGAYGARLTGAGFGGSAIALVDRERAEELGRAILGEYQRAFPWRAEYFVVEPSDGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P4Y5</accession>
    <name>A0A218P4Y5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">H/ACA RNA-protein complex protein Gar1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853108.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P4Y5"/>
    <dbReference type="GeneID" id="33314624"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00100"/>
    <dbReference type="OrthoDB" id="60264at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.230">
        <property type="entry name" value="Probable tRNA pseudouridine synthase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038664">
        <property type="entry name" value="Gar1/Naf1_Cbf5-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007504">
        <property type="entry name" value="H/ACA_rnp_Gar1/Naf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009629">
        <property type="entry name" value="PRK13149.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04410">
        <property type="entry name" value="Gar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="110" mass="11929" checksum="DB69B132391B1DA0" modified="2017-09-27" version="1">MKRLGKVSHYAKQGFLILRSDWVPALNSPVVDKGLTLVGTVKDVFGPVKRPYVAVKPRVRDPESYVGALLYVDSSRKSKPGKSKSGKAGRSGGKRSKGGKARRPAPRKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P511</accession>
    <name>A0A218P511_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Gufa protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_00260</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853139.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P511"/>
    <dbReference type="GeneID" id="33314656"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00260"/>
    <dbReference type="OrthoDB" id="11839at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF70">
        <property type="entry name" value="OS05G0316100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ05876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="269" mass="29083" checksum="F72B8CB842D3852A" modified="2017-09-27" version="1">MLENFITNLAEWILNLSNGSTMWVAFYAGLFVAMMTSLGALVAIFAKSLPEDGVDFSLSFAAGVMIVASFTSLILPAIEDTGSFSPAGIGIALGVLLIYAIDRLLPHEHLVRGYEGPKSMTERLRKVWLLVIAVIIHNLPEGLAVGTSLVYNLEVGLVTTIAIGIQDFPEGTVVSLPLAAIQKKRLQPILMGVLSGVAEMVMVLVGAYFFTLFSWLLPYGLGMAGGAMLYVTVKEMIPEIYRGEKNETLVTLGFFAGFYVMLFLDSMLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P539</accession>
    <name>A0A218P539_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Polyamine aminopropyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Putrescine aminopropyltransferase</fullName>
            <shortName evidence="3">PAPT</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Spermidine synthase</fullName>
            <shortName evidence="3">SPDS</shortName>
            <shortName evidence="3">SPDSY</shortName>
            <ecNumber evidence="3">2.5.1.16</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">speE</name>
        <name evidence="8" type="ORF">A3L08_00425</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 6">
            <text>S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12721"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="EC" id="2.5.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer or homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3 5">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.16" evidence="3"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853169.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P539"/>
    <dbReference type="GeneID" id="33314689"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00425"/>
    <dbReference type="OrthoDB" id="10538at2157"/>
    <dbReference type="UniPathway" id="UPA00248">
        <property type="reaction ID" value="UER00314"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.140.10:FF:000009">
        <property type="entry name" value="Polyamine aminopropyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="entry name" value="Spermidine synthase, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
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        <property type="entry name" value="SPERMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Spermine_synt_N"/>
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        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
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    <keyword evidence="3 4" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2 3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="7">
        <location>
            <begin position="11"/>
            <end position="245"/>
        </location>
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    <feature type="active site" description="Proton acceptor" evidence="3 4">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
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            <position position="91"/>
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        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
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        <location>
            <position position="111"/>
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        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
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        <location>
            <begin position="148"/>
            <end position="149"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
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    <feature type="binding site" evidence="3">
        <location>
            <begin position="166"/>
            <end position="169"/>
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        <ligand>
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            <position position="173"/>
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        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007867"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_00198"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ05906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="290" mass="33521" checksum="9DD54F00EE8D4A71" modified="2017-09-27" version="1">MGYNEQERAFIEWYPRGYGVGFKVTRRLFETQTDYQRLEIYETEGFGKLLVLDGTVQLVEIGEESYHEVLVHPVLLAHPNPKRVLVIGGGDGGTLREVLRHKTVEKATMVEIDDAVVEASYLYLDVAKDLLDRLIKNEEPRAELIIGDGVKYLRESEEKFDAIIVDSTDPVGPAKLLFSEGFYRDAYEKLNDRGLYITQAGSVYLFTNELLDAYRGMKKVFDRVYYFSFPVIGYASPWSFLVGVKGDIDFTKVDLERAKELKLHYYDPERHETLFQMPRYVRKLLEEQGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P541</accession>
    <name>A0A218P541_9EURY</name>
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        <submittedName>
            <fullName evidence="8">Cation:proton antiporter</fullName>
        </submittedName>
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        <name evidence="8" type="ORF">A3L08_00400</name>
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    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
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            <taxon>Archaea</taxon>
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        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NUOXDRDTASE4"/>
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            <end position="24"/>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="498" mass="52776" checksum="0C6ACC3945EFD413" modified="2017-09-27" version="1">MLPLMVASPLLTAFLIVLLDVLHAGKRAVQALFIAGAILPIPVAWLSGGGSEIVGGWPREAGIEVALTEVNTPFIVGELVLFTFVSIYSLQYFSFRDKKTPKVLALLLLVHTGLLGAFIARDFFNFYIYMEIASVSAFALVALSKESSAKRAAFKYLMLSLLASYMFIFSLGLIYMETGYLNAELASRTAVPSRELGAAIAIATASLLLKAGIFPLHSWLPDAHSRAPTPVSALLSGAVVKAPAYGMVLLFSSLPVGETLRTVLMGTAVASVFFGIGMALLQRDAKRLLAYHTVSQMGYVLLGIASLNFVGAAYYAMAHSIFKGGLFLAVGSISTKSRELGEFGYKNESVIAVSVLMLSLAIGGVSPFVGAYAKGLVSSGLSDGWKLAVLLGTIGTLISFTKLNYYLMRGQSPSLLPAWKLSSFGLALAAFLAGLLLGTGINLKDALNIAIAVISFAILKRTGLLEKRVRLSSGDVGKEINVLVAVFVAAVVLLLSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5A8</accession>
    <name>A0A218P5A8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Metal-dependent hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_00655</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853212.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A218P5A8"/>
    <dbReference type="GeneID" id="33314735"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00655"/>
    <dbReference type="OrthoDB" id="101433at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051916">
        <property type="entry name" value="GPI-anchor_lipid_remodeler"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14859">
        <property type="entry name" value="CALCOFLUOR WHITE HYPERSENSITIVE PROTEIN PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14859:SF1">
        <property type="entry name" value="PGAP2-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="371"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ05950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="578" mass="62415" checksum="E3C50A91FA830B1D" modified="2017-09-27" version="1">MRIEKRDKILLGLGAGVLLASSLRVFVAGAYSSLEKTFFYGVNFPSGAGILLFIIAAFLVGRMSRKAGAAIMALYALASLATDATEYTHLIAAFALPVALALVKEVEVRYLAIGLVADLSLRVLAVGGEPIDFPHTRAILALLLFLGAYALWREPGTLKKPGFGLYAFAALLELGLIYPNAIMRYSGFNVYYLPQFVGFSFVVALAILLGPYLSKRPGLAATLLVLGSASLFVKPAGLIGLPLALASTVALLESAKGSRGGVIGAVYLFLMATLALGAYVGRDIGLAFMEDRLEALILAASVVYALSAYKGNVEVELPSVKEIAGSLAGLVIVAAIVLALFNAGPSYEPAKKEVLIWTYNVHQGFGPYDGTFNGYELVNLIREQKPDVWAAQEVVGGMIGNGYQDVPLMMSAYLGYAYEYNPAVEGTYGIAVFSHWHMKTEGELNLKSIGQARPAQKVSIEELGITLVNVHMGLSEEERAMQAEELLSFAEASPAAQIIAGDTNAEPDERAIEILTRDYRDAFEERPPYTFNWGDVDIENIDYILLKKDWPAEVKDYGCLCDVEVSDHRPVWALVEIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5D0</accession>
    <name>A0A218P5D0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_00825</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853239.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5D0"/>
    <dbReference type="GeneID" id="33314769"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00825"/>
    <dbReference type="OrthoDB" id="34534at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044038">
        <property type="term" value="P:cell wall macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06856">
        <property type="entry name" value="GT_GPT_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926">
        <property type="entry name" value="PHOSPHO-N-ACETYLMURAMOYL-PENTAPEPTIDE-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926:SF3">
        <property type="entry name" value="UNDECAPRENYL-PHOSPHATE ALPHA-N-ACETYLGLUCOSAMINYL 1-PHOSPHATE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="207"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ05977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="302" mass="32286" checksum="1BC604D2B0233B60" modified="2017-09-27" version="1">MIAVAPLIGLTLTLILTPYLARMLKERGIIGKDIHKPEKPEVAEMGGLSILIGVSVALLPFAGQETARALAVFLLFGLVGIIDDLVALRQSHKVVLSLLVAVPVAFLDVSQEVDVFGYSLNLWFLYPLFALLFVVGSANLVNLLAGFNGLEIGTSAIALGFLAAITEGPTRMVALTGLGAALGFLWWNKYPARVFPGDTGTLSMGALIGLVGIIGKVEVYAAILLIPHFLDFTIKAIGVRFGVRKHGRTTVLPDGTLQAPPYPSFLGLIMRKVRVNEPKLVAIVWGVEFILGLLVLALRLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5D1</accession>
    <name>A0A218P5D1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CRISPR-associated endoribonuclease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_00830</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the CRISPR-associated protein Cas6/Cse3/CasE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853240.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5D1"/>
    <dbReference type="GeneID" id="33314770"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00830"/>
    <dbReference type="OrthoDB" id="43942at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049435">
        <property type="entry name" value="Cas_Cas6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010156">
        <property type="entry name" value="CRISPR-assoc_prot_Cas6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045747">
        <property type="entry name" value="CRISPR-assoc_prot_Cas6_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01877">
        <property type="entry name" value="cas_cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36984">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS6 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36984:SF1">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS6 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21350">
        <property type="entry name" value="Cas6_I-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01881">
        <property type="entry name" value="Cas_Cas6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005054">
        <property type="entry name" value="PF1131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CRISPR associated protein Cas6 C-terminal" evidence="5">
        <location>
            <begin position="117"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR005054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ05978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="249" mass="28726" checksum="AEB7415C189F03BA" modified="2017-09-27" version="1">MRVEIKFRPAEEGTILPFNYNHDVYAQLIEKIAIVSPEIAREAEVSHVDYFTFSRIMVRKRELIPDRGIRVLSDDVSLYVSSSSNELIKAVVEGFIDSPVLQLGEATFIAEDVKVLKEPKIGRDVLFSTLSPIMVRTVKLSGDRMKIWDLYPSEEAFFDKLRKVMLMRYSAIMGSMPEEKDFSIDVVKFKPVRILVKDTYYRGSLMIFRYTGSEEIARFGYENGFGEKTRYGFGMVKVIDEEQEREAPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5D9</accession>
    <name>A0A218P5D9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sugar transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_00875</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853249.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5D9"/>
    <dbReference type="GeneID" id="33314779"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00875"/>
    <dbReference type="OrthoDB" id="56622at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520:SF5">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="363"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="1"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ05987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="388" mass="42392" checksum="7A0B0963F2196114" modified="2017-09-27" version="1">MLAGYGRDAKILIAANAAGQLFLQFSVFIMPFYLRALGYDMSAMGTFFSIQTFTGGLFFLLAGQISLRMGYKKTLLLSAVLGLTGRLLQVAAINTYVLALGFFLVGANMGLRQPNFTALLSEEVGEERRHHAFSISFGLGTIFNAAGVLVAGFAPGFLTGLGISEGTAYRLVISLALLQFVFVIPALLMISDVPVKNPRINWNRELVIKILKFSFPSALIGFGAGITIPYMSLYFNIRFGQTLAAISGIFFFQQLAMGLGSFALPRLVNRIGPVKVITSFQSTAAFLFAIFPSVETFLLAAFLYVLRSILMNIVWPINDSFMMGFFSTEEKATAAGIRRAFSTFMRGGGNYVGGLLFATSLSYPFYATALLYVVATAIFYAFFIKHNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5E1</accession>
    <name>A0A218P5E1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pterin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00955</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853261.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5E1"/>
    <dbReference type="GeneID" id="33314795"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00955"/>
    <dbReference type="OrthoDB" id="103621at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042558">
        <property type="term" value="P:pteridine-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pterin-binding" evidence="1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="246" mass="28539" checksum="B9201E7B0F9DB6C3" modified="2017-09-27" version="1">MNVEDIKARLSRLESLHSAFEEKFPLLYGENDRERFVEILRGLHTISREKLELSSALYREMVGSTYAENQAKELYRNEHQMKFRIEELLSLLAKEDYDAKLKLSTAMDRLAQFHRVYDYAVRKALSELAREVEGLELLAGGENQKKVPVGILEELRKIKTLEAELEALKRFLFRLYAHPGDVHKVEEALRDWHSRGLLWVEARNVEKLSGVRNAEEILEGLALIGVVEKKMRGGEGVYRHRSYSPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5E6</accession>
    <name>A0A218P5E6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">tRNA (Guanine-N1)-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_00970</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853264.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5E6"/>
    <dbReference type="GeneID" id="33314798"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00970"/>
    <dbReference type="OrthoDB" id="8079at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000899">
        <property type="entry name" value="tRNA (guanine(37)-N1)/4-demethylwyosine(37)-methyltransferase Taw22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.110">
        <property type="entry name" value="Met-10+ protein-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056744">
        <property type="entry name" value="TRM5/TYW2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040601">
        <property type="entry name" value="Trm5a/b_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047728">
        <property type="entry name" value="tRNAMtaseTaw22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF36">
        <property type="entry name" value="TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18093">
        <property type="entry name" value="Trm5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25133">
        <property type="entry name" value="TYW2_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="6">
        <location>
            <begin position="90"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="337" mass="39399" checksum="E416E1F7DD29DB9D" modified="2017-09-27" version="1">MPVLKVPKREAEPVKRKLKKLNLYDGKRRPGIEGDFVLLPIFEDETVYSLGYEVLDIELPLRPERQIYKNLESVLAERLTPEELKHLRRYDVIGDIAVIQIPPELEHRVDDIVWGLRKVHPFLKVVAKKGFHEGAFRIRDYSIIWGEKRLETVHKENGVEIKVDLSKAFFNPRMKGERYRLAQLVRDGERILIPFAGVLPYALVIARYRKVKITAVELNREAYELGLENIELNRKRLKGEIEFIHGDAFEVLPELPTYDRVISPTPRGVDALALTLSKAERWLHYYDFVHEAEIEDFRRRIIEECSRQGKDCEVRVKKVSDFKPHVFKVCADVRIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5J7</accession>
    <name>A0A218P5J7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">RNA methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_01260</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853315.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5J7"/>
    <dbReference type="GeneID" id="33314856"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01260"/>
    <dbReference type="OrthoDB" id="372184at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002128">
        <property type="term" value="P:tRNA nucleoside ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18093">
        <property type="entry name" value="SpoU-like_TrmJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004384">
        <property type="entry name" value="RNA_MeTrfase_TrmJ/LasT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00050">
        <property type="entry name" value="rRNA_methyl_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42786:SF2">
        <property type="entry name" value="TRNA (CYTIDINE_URIDINE-2'-O-)-METHYLTRANSFERASE TRMJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42786">
        <property type="entry name" value="TRNA/RRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004808">
        <property type="entry name" value="LasT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="tRNA/rRNA methyltransferase SpoU type" evidence="5">
        <location>
            <begin position="4"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="239" mass="26939" checksum="4432F0EB372BF0A7" modified="2017-09-27" version="1">MDASVVLVEPEGPANIGMVARTMKNFGFSRLVLINPNLTEESYSYAVHAKDILENALVLEEFGEALELFDLAVGTTGKPGKNYIPDRAPLMPWELRETLEGYLGRVGLFFGRESIGLRNEELAKLDFTVTIPTSDSYPIMNLAQAVAVVLYELAKKRPEPAVEALTPATRKEKEQLVKTWAELLEVLNYPKDAERREVFVKVFRRFVGRAILYGREVHTLMGPLRKARLRLEECGDAER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5L6</accession>
    <name>A0A218P5L6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01430</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853347.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5L6"/>
    <dbReference type="GeneID" id="33314891"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01430"/>
    <dbReference type="OrthoDB" id="18248at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035241">
        <property type="term" value="F:protein-arginine omega-N monomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007364">
        <property type="entry name" value="SFM1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35517">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE SFM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35517:SF1">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE SFM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04252">
        <property type="entry name" value="SFM1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="211" mass="24356" checksum="35F320A464428B76" modified="2017-09-27" version="1">MPYLLIEHLEELRDWVLLEYRHASEWWGERLIFTNVEAHEREELAKLGSVLTQSVTEFPFDRSKLIILDPFAEEELKPEDIEEDSIIVVGGILGDFEFTGKTKKFITERIEGAKARHIGSVQFSIDGSAIVAKFIAEGKRLEEIEYELNPTIKLDEFSEITLHYAVPKLNGKLLLTPGLIELQKRELGYMEADEEISDDELIEFFEGKREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5M1</accession>
    <name>A0A218P5M1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TIGR00153 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_01250</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0111 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853313.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5M1"/>
    <dbReference type="GeneID" id="33314854"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01250"/>
    <dbReference type="OrthoDB" id="68479at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="entry name" value="Phosphate transport system protein phou homolog 2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002727">
        <property type="entry name" value="DUF47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018445">
        <property type="entry name" value="Put_Phosphate_transp_reg"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00153">
        <property type="entry name" value="TIGR00153 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36536">
        <property type="entry name" value="UPF0111 PROTEIN HI_1603"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36536:SF3">
        <property type="entry name" value="UPF0111 PROTEIN HI_1603"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01865">
        <property type="entry name" value="PhoU_div"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109755">
        <property type="entry name" value="PhoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="37"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="136"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="215" mass="24112" checksum="ED0CF6450AD0AE01" modified="2017-09-27" version="1">MPIFGGKESSVFETIEDHLKVVNESLVAFRELMVAYLEKDLERAKAFEREVDKLESKADTLRRSIETMLYEGAFLPANRGDYVRLSELIDQVADAAESAAHTLVLARPKIPEELKDEILGLVDSAIETYKVLIDAVNALNSDVDRAIELAKAVEDAEEEADDVEYEVKSKVFESETITTYAKLIWNQILTKIGDIADRAEDASDQVMLMAIKRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5M2</accession>
    <name>A0A218P5M2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6 7">Carbamate kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_01450</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the carbamate kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853351.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5M2"/>
    <dbReference type="GeneID" id="33314895"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01450"/>
    <dbReference type="OrthoDB" id="31128at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008804">
        <property type="term" value="F:carbamate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019546">
        <property type="term" value="P:L-arginine deiminase pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04235">
        <property type="entry name" value="AAK_CK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000007">
        <property type="entry name" value="Carbamate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003964">
        <property type="entry name" value="Carb_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00746">
        <property type="entry name" value="arcC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009007">
        <property type="entry name" value="PRK12352.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009008">
        <property type="entry name" value="PRK12354.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30409">
        <property type="entry name" value="CARBAMATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30409:SF1">
        <property type="entry name" value="CARBAMATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000723">
        <property type="entry name" value="Carbamate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01469">
        <property type="entry name" value="CARBMTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/glutamate/uridylate kinase" evidence="8">
        <location>
            <begin position="2"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="NCBIfam" id="TIGR00746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="315" mass="34171" checksum="2FFF04DD1B9053EA" modified="2017-09-27" version="1">MKRVVIALGGNAILQRGQKGTYEEQMENVTKTAKQIVDIILDGDYEVVITHGNGPQVGALLLHMDAGQAVHGIPAQPMDVAGAMTQGQIGYMIQQAIVNELRRRGVERPVATIVTQTLVDKDDPAFQNPSKPVGPFYDEETAKKLAREKGWTVIEDAGRGWRRVVPSPDPKGHVEAPVIVDLVEKGFIVIASGGGGVPVIEENGQLKGVEAVIDKDLAGERLAEEVKADIFMILTDVNGAAVNFGKPNEKWLERVTVDELRRYYEEGHFKKGSMGPKVLAAIRFVEWGGERAVIASLDRAVEALEGRTGTQVVGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5M4</accession>
    <name>A0A218P5M4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853361.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5M4"/>
    <dbReference type="GeneID" id="33314906"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01505"/>
    <dbReference type="OrthoDB" id="102096at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="239" mass="26307" checksum="962D36B2E15446A0" modified="2017-09-27" version="1">MLTPERAIEYYFAIISVLGSLVVLLSIKYTLRRWRSFPETGWVPQAIAMGVLGLALASIIELPAMLLKAWVTLAFLAGMTEESVKLLPLRFFGSFDGWARWKLVIGTGLFFGLIEGIFYTTWIFALGQEWHLIIVRLVLIGLHTIWAAISVGFLLGGNGLKRFTGLAFSMVAHALYDLPSLAVLQSYSGNTVAYLAGLSTGFLLITPLMARKTAELTGALVPAEEKEEDPEVETFTFSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Q5</accession>
    <name>A0A218P5Q5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Deoxyribonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_055430018.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5Q5"/>
    <dbReference type="GeneID" id="74506641"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01730"/>
    <dbReference type="OrthoDB" id="28569at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRAM" evidence="1">
        <location>
            <begin position="13"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="70" mass="7580" checksum="236A8991C88AF630" modified="2017-09-27" version="1">MYGDGFGGGYEAPVKVGERYRVRIESLGKGGDGIAKIKGFVIFVPNTQVGDEVEIVINSVKRKFAFAEVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5T3</accession>
    <name>A0A218P5T3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853417.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5T3"/>
    <dbReference type="GeneID" id="33314970"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01825"/>
    <dbReference type="OrthoDB" id="111026at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049945">
        <property type="entry name" value="AAA_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052026">
        <property type="entry name" value="ExeA_AAA_ATPase_DNA-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35894">
        <property type="entry name" value="GENERAL SECRETION PATHWAY PROTEIN A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35894:SF1">
        <property type="entry name" value="PHOSPHORIBULOKINASE _ URIDINE KINASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ORC1/DEAH AAA+ ATPase" evidence="1">
        <location>
            <begin position="64"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="401" mass="46473" checksum="7A4B455015DD647C" modified="2017-09-27" version="1">MESGGLKLYPYQSYEVYGLSRNPFEQLASEGITDIESIHVYQEVDMRLQMIVSEVIGNKSSIALSIVGPLGMGKTQRLKSLAKAIEENRGKAIYVKVDTNDILKLTRDIFYALKPPKSRTNIFLENLSKKLGFIDRLEKMLSDTKEYKSRDIAELLVEQMSKYPYCALLLDELENMQSAREHEKIQFFEMLRHFISTMPKGCIVAFASVPEAYEEYTKIFPAFFMRLHYEFRLRPMSLDETFELVKKRLNKVRVRDTDDPIYPFTEEAIKLIHQLGKGNPRQILRLLHYVLSEAAKHKFDPIDDYVVTTILEEPKSLEEYLTRIPKEYKDLVEAIVFEFNGGPVSYIQVAKAVKKPGMQVYDQLNDLIRLGFLVGDPKGNYKVPDYVRKFLEEGQAQKEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5T9</accession>
    <name>A0A218P5T9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">CO-induced hydrogenase subunit K</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_01795</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853411.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5T9"/>
    <dbReference type="GeneID" id="33314964"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01795"/>
    <dbReference type="OrthoDB" id="15253at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052561">
        <property type="entry name" value="ComplexI_Subunit1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001694">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1/FPO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43359">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43359:SF1">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00146">
        <property type="entry name" value="NADHdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="275"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="330" mass="36358" checksum="8BA948D1A872F49A" modified="2017-09-27" version="1">MDVVGELVYPVAGLLGLYVFVSLTSLVWEGIDRKLVARMQRRIGPPILQPIYDFLKLASKETIIPNTANFMFRAAPVLALATAIALLAYTPMGFAPILASKGDVIVFIYLLTLIGFFKILGAISSGNPYAKIGAAREAAIMVSREPAMMLAVFAIMWRLGKLGVPKPFSMEIFYTHNIWEIGTPMSFVGALILLYVFCVWLASEIEVGFFNIPDAEEEIAEGLLVEYSGRYLALLKLTHSLKAFISASLVVAIFFPWGISGYFNLTGLPAQVVDLLFHTLKVFALLFVVQSVFRAVTGRLKITQAVDFLWKNVFLASLIGSLLIAMEVIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5U1</accession>
    <name>A0A218P5U1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Biotin synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_01890</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853429.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5U1"/>
    <dbReference type="GeneID" id="33314983"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01890"/>
    <dbReference type="OrthoDB" id="35347at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43288">
        <property type="entry name" value="BIOTIN SYNTHASE-RELATED PROTEIN, RADICAL SAM SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43288:SF2">
        <property type="entry name" value="RADICAL SAM CORE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01113">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Elp3/MiaA/NifB-like radical SAM core" evidence="5">
        <location>
            <begin position="16"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="278" mass="31824" checksum="AC410DC9BF102667" modified="2017-09-27" version="1">MTERKKLKIYIPGIKFPSVSVTGNACALNCAHCGKHYLEGMRKPERGELLSYCLDLSRKGYAGCLLSGGMDGRMKVPLDFYADEIREIKRRTNLKLNAHVGFIDENDLEWLRWVDVVSLDFVGDDDVIKRVYKIDKTVKDYLRILDILTENGIRVAPHITIGLDFGKIHWEYKTIDLLMNYPIDVLVLDVLIPTKGTEMENVPKPSVEESLKVVKYARERFDGEISIGCMRPLGKWRLDFDRGAVLAGVDRLTNPPRKIIEWAKTVRDVEVIYECCVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5U5</accession>
    <name>A0A218P5U5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NADH-plastoquinone oxidoreductase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_01800</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853412.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5U5"/>
    <dbReference type="GeneID" id="33314965"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01800"/>
    <dbReference type="OrthoDB" id="23833at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3270:FF:000014">
        <property type="entry name" value="NADH dehydrogenase subunit I (NuoI)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050157">
        <property type="entry name" value="PSI_iron-sulfur_center"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006221">
        <property type="entry name" value="PRK08348.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24960:SF79">
        <property type="entry name" value="PHOTOSYSTEM I IRON-SULFUR CENTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24960">
        <property type="entry name" value="PHOTOSYSTEM I IRON-SULFUR CENTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="37"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="68"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="141" mass="15835" checksum="EBEE9348673E14DC" modified="2017-09-27" version="1">MRAPPLVPTVLRNLFRKPATNPFPKTEPVPIPEDFRGMITYNVDKCVGCRMCVNVCPAGVFVYLPEIRKVALWTARCVYCSQCVDVCPTGALQMSRDFLLASYDNKAGRFIPLKPEKVEELKKKVEEAKKAKETAKKEAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Y2</accession>
    <name>A0A218P5Y2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">acetate--CoA ligase (ADP-forming)</fullName>
            <ecNumber evidence="2">6.2.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_02115</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP + CoA = acetyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15081"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="6.2.1.13" evidence="2"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853470.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5Y2"/>
    <dbReference type="GeneID" id="33315028"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02115"/>
    <dbReference type="OrthoDB" id="18103at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043758">
        <property type="term" value="F:acetate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051538">
        <property type="entry name" value="Acyl-CoA_Synth/Transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043938">
        <property type="entry name" value="Ligase_CoA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032875">
        <property type="entry name" value="Succ_CoA_lig_flav_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43334:SF1">
        <property type="entry name" value="3-HYDROXYPROPIONATE--COA LIGASE [ADP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43334">
        <property type="entry name" value="ACETATE--COA LIGASE [ADP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19045">
        <property type="entry name" value="Ligase_CoA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13607">
        <property type="entry name" value="Succ_CoA_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA-binding" evidence="6">
        <location>
            <begin position="9"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="469" mass="50960" checksum="6DCED1A4E0CB340E" modified="2017-09-27" version="1">METPNLDFLFYPKSVAVIGASNVPGKIGNAIMRSITLRFEGRVYPVNVRGGEIEVNGKKFPVYKSIHEIPDEIDVAVIAVPAKFVPDVIDDCGKKGVKAAVVISAGFKEAGRTDLEEELVKRARKWGIRLVGPNCLGVTNLENGFDCNFNPPERQARPPFGKVAFMSQSGAFGAAILDWAASHKIGMSKFISLGNMADLDESDFMAYLGDDPKTGVITAYIEGVKDGRKFFNTAKQVTLKKPVVILKSGRTEAGAKAAASHTGSLAGSYRIYQAAFEQAGVLEAKSMRQLFNYAKALAMQRPAKGNHVAIVTNGGGAGVMMSDGLLERGMKLAELSEETNEKFRRDIGAGKLPEHMSYRNPIDVIGDAPSSRYEIAMRYALEDPNVDVLVVIALFQSPALDEGIVEAMERMKAYGKPIVFVAPGGDYPHKMARNIELKGIPVYETVEDGVDAVYALVRYGEWLKENGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Z9</accession>
    <name>A0A218P5Z9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_02125</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853472.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5Z9"/>
    <dbReference type="GeneID" id="33315030"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02125"/>
    <dbReference type="OrthoDB" id="100203at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="247" mass="27778" checksum="03337097BE2858A1" modified="2017-09-27" version="1">MKLVVLRERGSTVALAFMVVFMVIFLAMFGFMLVSVQGGSVILLGFFAFFMAMMLFGVYALLRKRWEYRRVQNFAEMVSFSDSSVSFPESLEFEVGRLEMRGYWVGSGRNRSYRVERKFTTERSGRSQGIAFPEGEFKVAIDADGSGIVTAPAVRLLSDPYKDVLLIFLTDSGRVVGEWSIQLSHNGDSAEVVFKGEGKAVTGHVQAYLSKARKVRWKSRHRGPEERSSARASTSSSEGAYFLRRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P649</accession>
    <name>A0A218P649_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphatidate cytidylyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_198362128.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P649"/>
    <dbReference type="GeneID" id="33315084"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02395"/>
    <dbReference type="OrthoDB" id="107330at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037997">
        <property type="entry name" value="Dgk1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31303">
        <property type="entry name" value="CTP-DEPENDENT DIACYLGLYCEROL KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31303:SF1">
        <property type="entry name" value="CTP-DEPENDENT DIACYLGLYCEROL KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="208" mass="23435" checksum="F4A61160760B735C" modified="2017-09-27" version="1">MSMKSEIRRKSLHMTGLIVPLLYYLFGREFTLTFVAIAFFIFVILEPFRIIEELRDRIKRRLRIYVDSDVFERVEAIEKQIDEITREHERFRVAAHIYFAAASFIVVYFFPMNVAVGAITVATVSDALAAVVGKPFGRHRFSNGKSLEGSLAYFFSGVAILWSLVGLPLAIIGSLVGVITEFYNLPPDDNFSNQLAIAAVIYLASAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P669</accession>
    <name>A0A218P669_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endonuclease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_02490</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853538.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P669"/>
    <dbReference type="GeneID" id="33315103"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02490"/>
    <dbReference type="OrthoDB" id="88281at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="14001" checksum="08BDFCF9629E2707" modified="2017-09-27" version="1">MSKIKVKTLTREIVSLAEGLGLKAVPEYRTPDGTRIDIAVLNGEKKLLAIELEASFKWFPQRLLYDVVKAHRAGFPELWVITPFRNTKPGWVLNYAREIGIGLRILKEKEVIEEFRVLLARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P675</accession>
    <name>A0A218P675_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA uridine(34) acetyltransferase</fullName>
            <ecNumber evidence="14">2.3.1.311</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Elongator complex protein 3 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">A3L08_02500</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">tRNA uridine(34) acetyltransferase, which mediates formation of carboxymethyluridine in the wobble base at position 34 in tRNAs. The proposed mechanism is the following: (i) recruits S-adenosyl-L-methionine and cleaves it to generate a 5'-deoxyadenosine radical (5'-dA) in the radical S-adenosyl-L-methionine (rSAM) region, (ii) hydrolyzes acetyl-CoA in the N-acetyltransferase domain and (iii) an acetyl radical is formed by the products of the two domains and (iv) is transferred onto the C5 position of uridine(34) in the bound tRNA molecule. Does not show protein lysine acetyltransferase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>uridine(34) in tRNA + acetyl-CoA + S-adenosyl-L-methionine + H2O = 5-(carboxymethyl)uridine(34) in tRNA + 5'-deoxyadenosine + L-methionine + CoA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:61020"/>
            <dbReference type="Rhea" id="RHEA-COMP:10407"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74882"/>
            <dbReference type="EC" id="2.3.1.311"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:61021"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="18">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ELP3 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.311" evidence="14"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853540.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P675"/>
    <dbReference type="GeneID" id="33315105"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02500"/>
    <dbReference type="OrthoDB" id="49957at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106261">
        <property type="term" value="F:tRNA uridine(34) acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000388">
        <property type="entry name" value="Histone acetyltransferase Elp3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000211">
        <property type="entry name" value="Histone acetyltransferase Elp3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034687">
        <property type="entry name" value="ELP3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056591">
        <property type="entry name" value="ELP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01211">
        <property type="entry name" value="ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF7">
        <property type="entry name" value="TRNA URIDINE(34) ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23613">
        <property type="entry name" value="ELP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005669">
        <property type="entry name" value="Hist_AcTrfase_ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01086">
        <property type="entry name" value="elongater_protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00344">
        <property type="entry name" value="ELP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11 18" id="KW-0408">Iron</keyword>
    <keyword evidence="12 18" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Radical SAM core" evidence="20">
        <location>
            <begin position="72"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="19">
        <location>
            <begin position="419"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00056275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00076679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR005669-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ASJ06278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="591" mass="69226" checksum="18AD0FD806584AD4" modified="2017-09-27" version="1">MNGEDFKKAVEEIARAVLSGEIKSREELNRYKIIVSRKYHLSKIPGNSEVLKAIPEEERERFRELLKRKPTRTISGVAVVAMMTKPFPCPHGRCIYCPGGPAIGSPQSYTGKEPSALRAVQSAYHPYIIMMRRLKQLTDIGHDVDKVEVIIQGGTFPAVDLDYQEWFVKCAFKAMNDFPHFRDIENLEEKLVRLIVKRDESVFEEDPKFKEAWLKTHRRPYYYLEEEQRKNEKARVRMVGLTIETRPDWAFERQIDRMLRFGTTRVELGVQTVFNFIHERTKRGHGVEEIIKATQLLRDAGLKINYHIMPGLPGSNFERDLHTFRAIFEDPRFRPDMLKIYPTLVTADAPLYRWWKEGKYRPYTTEEAVELLVEAYKYFPKWVRVMRIQRDIPVQLIVDGVKHSNLGQLVFNELIKRGIRPREIRFREVGHQMEKFGVEPEVEHIKLLREDYDAAGGREIFLSFEDIKNDILIGFIRLRIPSEKAPRKEINCCPSAIVRELHVYGPLVPIGGKPRYEWQHRGYGRELLSEAERIAKEEFDVKKMLVISGVGVREYYRKFGYRKNGPYVAKKLDKGYADYKKSKDFDAHLNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P692</accession>
    <name>A0A218P692_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P692"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02680"/>
    <dbReference type="OrthoDB" id="98599at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="158" mass="17509" checksum="B12B41D433C48EF4" modified="2017-09-27" version="1">MGTLRDAMGYPVLRAGIILLVLALLISIAGLYRVDKSYSSSGTLGEGMHYLGDDKFENEYLYHNRTLTLSSTNATISLLQGSEMYNYTLVNDSVTLHPEMRPAIYVFNGEVNYTYDARAIDYPYAVYSIGAFVIMLAGIALSFIGYTQFLKDVKGGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6A4</accession>
    <name>A0A218P6A4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Peptide ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_02655</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6A4"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02655"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904680">
        <property type="term" value="F:peptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.105.10">
        <property type="entry name" value="Dipeptide-binding Protein, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290:SF9">
        <property type="entry name" value="OLIGOPEPTIDE-BINDING PROTEIN APPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PERIPLASMIC BINDING COMPONENT OF ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="773"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="domain" description="Solute-binding protein family 5" evidence="7">
        <location>
            <begin position="44"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="746"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="238"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="746"/>
            <end position="762"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="795" mass="89299" checksum="D74B07D8FC970153" modified="2017-09-27" version="1">MGLMLFSLFAIHPVSAADYTPKDIPLNSDEAKARFKADLEWYLNYGHFVISNGPYILEMYSPENLYLKLVKFNGQRNTFNDDPKLPKDGYADVVEYQGVQNPENVILQIAKGEYDLGLFAFGANKYQGLGSDVLSKLNLYKSASSSVELTINPYKDPDKDAPIVTVGDNVYFNPFAIREVRFAMNWLISRNYLIQNIYQGSGAPALSGITPSDPAAKYFEPVYQALKFTADGNEALALQMIDEAMQKAKEQVAKYNHKLEKKDDGLWYFDDQPVTVKFVIRTEDERKDVGLYIADLLEKKVGFKVDRMLLDRQKASEIVLDKPITTYEWNLYTGGWGAGGLGSMYPDWQIYYWYSPLGYYPNYIDPRHEPEVTVEDALKYIGDGSVAAGLQKLQTKYYTSEESLGPILNWTTREIGYILLMTQYTDPATNTTIVLNNPDQYWDFQKIGMTAGLMESIRIFLVEQWEFYPVNKERVTDIISDDSIGIASRWSIMSAKTPDKHLKVAQFASTGALFMSAFNPIGGISDVYSTRLWNLIHDTGGTINFDGIYVPYRCKWTLEKGEFTVPDDAVIYNQTQGWIAAHKGETANAKVTVTCDLGEWHNGVKMTIDDIKYYIAFYYAWAFQDTPDDPYYDSSLSDAAATLQTYLGFQFTDDGYVVYGNYVHPFADDLTAGNYIMYPSLPWELYWTMGELVANGDAYGASSKYSFSSSGEGLLQLDLLTKQHVNDLAAVIQKISGINLDDLTKTTTTTSSQGQTESPTTTTGGGEGSNTTTYVVVGLVIIIIAAATWYFAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6A5</accession>
    <name>A0A218P6A5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Drug/metabolite transporter 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_02690</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
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            <taxon>Thermococcaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000197418">
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EamA_dom"/>
    </dbReference>
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        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF79">
        <property type="entry name" value="MOBA-LIKE NTP TRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="123"/>
            <end position="141"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
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            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
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        <location>
            <begin position="3"/>
            <end position="137"/>
        </location>
    </feature>
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            <begin position="146"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="290" mass="32215" checksum="5F4DA7ADC772042C" modified="2017-09-27" version="1">MRRGYLFVFLAASMWGTLGIFAKYLDGFGLSTFTMVFYRVLFAVALLAVYLRIRGIGFSIERSRLKFYALYGFFSIFLFYTLYFYTVTISSVSFAVLLLYTAPVYSIVLGKLIFGEKLTGEKVLALFLVMAGVVLVNGVNSGFSTKALLFGLLTGFTYALYGVLAKFAVRNEEPEKALFYTLLFGLFFLLPFTDFNVPAGAVPYLFALAFFPTFLGYILYNHALKEVEVSRASIIATVEPVVAITLAFLLFGETLSPRQIIGAALIIGGSIIVHAREKERLEEAALEEGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6B4</accession>
    <name>A0A218P6B4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02705</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6B4"/>
    <dbReference type="GeneID" id="33315147"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02705"/>
    <dbReference type="OrthoDB" id="86035at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="91" mass="9851" checksum="4B796F259CBD1231" modified="2017-09-27" version="1">MDEETKVLRDYLIFTVPHVTVLAGAVLGVLMIVGIPVNVALGIFAILYGLMLTILGLIIRPHVSGNTVYRLSMAFFVSLMIVGVIILFYGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6C4</accession>
    <name>A0A218P6C4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">rRNA methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_02755</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the methyltransferase superfamily. RlmI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853589.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6C4"/>
    <dbReference type="GeneID" id="33315157"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02755"/>
    <dbReference type="OrthoDB" id="190449at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21153">
        <property type="entry name" value="PUA_RlmI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11572">
        <property type="entry name" value="RlmI_M_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.80">
        <property type="entry name" value="RNA methyltransferase domain (HRMD) like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041532">
        <property type="entry name" value="RlmI-like_PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019614">
        <property type="entry name" value="SAM-dep_methyl-trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42873">
        <property type="entry name" value="RIBOSOMAL RNA LARGE SUBUNIT METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42873:SF1">
        <property type="entry name" value="S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10672">
        <property type="entry name" value="Methyltrans_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17785">
        <property type="entry name" value="PUA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PUA" evidence="9">
        <location>
            <begin position="2"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="396" mass="44678" checksum="02D2E4D7FFCF8EB9" modified="2017-09-27" version="1">MARVIVDAQAARAIGKGAMIVFKKGVVRTEGEFSPGDIVEVYTRGGKFLGKGFVNPHSNIMVRLITKDRETEVNKELFRERIKKANEYRKKVLGYDKAYRMVYGEADYLPGLIVDRFNEIASVQISSVGMERFKMEVAEAIMEAEPEVETVFEKNTGRSRRREGLPEIERVLLGKEKYRTIIEEGKAKFIVDMRGQKTGFFLDQRENRIALEKYVKPGMRVLDVFTYTGGFAIHAAVAGADEVVAVDKSPWAINMVKENAKLNGVEDKMKYIVGSAFPVMEEMIKKGEKFDIVILDPPAFVQHEKDLKRGLRAYFNVNYAGLQLVKEGGILVTASCSQHVDMQAFKDMVIAAAAKAGKFLKMLEPYRTQAPDHPILMASKDTEYLKALFLYVEDTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6C6</accession>
    <name>A0A218P6C6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_02830</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transcriptional regulator TrmB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853604.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6C6"/>
    <dbReference type="GeneID" id="33315172"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02830"/>
    <dbReference type="OrthoDB" id="30795at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09124">
        <property type="entry name" value="PLDc_like_TrmB_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051797">
        <property type="entry name" value="TrmB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021586">
        <property type="entry name" value="Tscrpt_reg_TrmB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047390">
        <property type="entry name" value="TransRegTrmBL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293:SF1">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11495">
        <property type="entry name" value="Regulator_TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159071">
        <property type="entry name" value="TrmB C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="5">
        <location>
            <begin position="10"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription regulator TrmB C-terminal" evidence="6">
        <location>
            <begin position="112"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="340" mass="39342" checksum="4A6AAAF019433B49" modified="2017-09-27" version="1">MHEEEIVEKLQKLGLTKYESLAYITLLKLGPSKATDITKESGIPHTRVYDVLSSLHRKGFVDVMQGSPRLYKPVNPEVVLERIKEDFIEDIEKLKAAFLDLYREVHGEDLPEIWTIQGFDNTVERAEYVIRTAKHEVLINTPFEFLKLLKSEIRARKDVLFVIISNFDEVPDWLKRSNIILAKTGGAPWLMASWIIGDIDYALFFGALPKDKRREKFYSFWAKSPKIIQNYMHWFYTIYFDNSEIIKPLDYGKLPKPLALVNIRTLITVLRMANLPKRAEIVGRIVDTKEPVTLDGEIIDYEYTPLTANITFKYNGNELKVGGIGSYFEDVEGEKFILLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6D3</accession>
    <name>A0A218P6D3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Sugar transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_02810</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. MalFG subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853600.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6D3"/>
    <dbReference type="GeneID" id="33315168"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02810"/>
    <dbReference type="OrthoDB" id="45815at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050901">
        <property type="entry name" value="BP-dep_ABC_trans_perm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32243">
        <property type="entry name" value="MALTOSE TRANSPORT SYSTEM PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32243:SF50">
        <property type="entry name" value="MALTOSE_MALTODEXTRIN TRANSPORT SYSTEM PERMEASE PROTEIN MALG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="325"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="206"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="416" mass="45450" checksum="F9D807486CB3C67D" modified="2017-09-27" version="1">MMGRRKSEILKSFTLTLVAILVMFIILFPVYYIFTVSISPGSTLATTEFHLIPRNVSLDSYREVLFGFSGNKLSEDFTGTIEGSASIQDGKLYLTEGTIRGKVKYGPFTGMTFEIPVKNLVFDVSGNSNAQGGLKGEVRGLFVLTRMNPDGTIGFGLLRNVILTEGSLDGTAVSGPMEKYVVARNSGTVHFTYLGKFVNSKFFGYLKNSLILATLTVILTLIFVVPAAYAFSRMKFFGREHVLYFYLMFTQVSGGLGIAGLIALYGMVVKLGLYDKLPVLSFIYAAGSVPFNTWLLKGYIDSISPDFDEAALVDGAGYLQIIRHVLLPMALPGIATVAIFAFIGGWTEFILASLLLTQDNQPLSVWIYLLMGGIGRGIDWSYFAAAALLFALPVFVMFMLAQNYIKSGLTVGGLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6F8</accession>
    <name>A0A218P6F8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853637.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6F8"/>
    <dbReference type="GeneID" id="33315210"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03020"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="142" mass="16116" checksum="A7E88F384FF2FBD8" modified="2017-09-27" version="1">MKAEQIILYIREGQASIEVLSPGKAWQGVTELELPLGPFVALVAETLGTGLKRGEAVFRYTRAKNTLRMDLNRLKVEVSPKRAFRLKLQNGRYPRKFLAVLVVGTGDIYDYLPGILEKRRKYIRFELSADDGSRRVYTLSIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6G1</accession>
    <name>A0A218P6G1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alpha-glycosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_02825</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6G1"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02825"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11338">
        <property type="entry name" value="AmyAc_CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02857">
        <property type="entry name" value="E_set_CDase_PDE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004185">
        <property type="entry name" value="Glyco_hydro_13_lg-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357">
        <property type="entry name" value="ALPHA-AMYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357:SF210">
        <property type="entry name" value="MALTODEXTRIN GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02903">
        <property type="entry name" value="Alpha-amylase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="3">
        <location>
            <begin position="213"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="630" mass="73456" checksum="5E269ABE88E763B6" modified="2017-09-27" version="1">MMKVYKTFGFEYDWRFGRVALVEFSTPLRGKWAYLVGSFNAFNEGSFRMEPRGGRWTIKVRLPEGVWHYGFSIGGEFERDGENPREETYRRLSYKFKRDVSVAVIDAGDWIFHAPSTTYVYTIAGRTHVLLRGKHGVIKWAELKTGGETFRMRKKAESELFEYFEVVLPREGEIEYSFEILTLRGKTVELGPFRTAPYKPDAPDWVFDRVFYQIMPDRFAVGIRGKTLPLEGEFFHGGDLKGIVEHIDHLESLGVNALYLTPIFESMTYHGYDIVDYYHVASRMGGDEAFEELVNALKERGMKLVLDGVFHHTSFFHPWFQDVIRKGEESEYRDFYRITGFPVVSNEFVEALKSDEPWVEKYTKLKGMDWNYEGFFSVWLMPRLNHDNPAVREFVVEVMEHWLREGADGWRLDVAHGVPPDFWRRVRRGMPATAYLFGEVMDDARLWILDVFHGTMNYPLYELLLRFFVEREIDAEEFLNGLELLSARLGPAEYLMYNFLDNHDTERFIDLVGDERRYLCALTFLMTYKGIPSIFYGDEIGLRGQRGPGLSVGRTPMIWDSRKWNGDLLEATKRLIALRKKSRAIQLGAFVPVKFEGDEIVYDRVLGDEGVRVVITYPKSGECSFEVRFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6K6</accession>
    <name>A0A218P6K6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nicotinate-nucleotide pyrophosphorylase [carboxylating]</fullName>
            <ecNumber evidence="9">2.4.2.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Quinolinate phosphoribosyltransferase [decarboxylating]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">A3L08_03210</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Involved in the catabolism of quinolinic acid (QA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>nicotinate beta-D-ribonucleotide + CO2 + diphosphate = quinolinate + 5-phospho-alpha-D-ribose 1-diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12733"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 9">Hexamer formed by 3 homodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the NadC/ModD family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.19" evidence="9"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853667.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6K6"/>
    <dbReference type="GeneID" id="33315248"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03210"/>
    <dbReference type="OrthoDB" id="115072at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00331"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034213">
        <property type="term" value="P:quinolinate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01572">
        <property type="entry name" value="QPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1170.20:FF:000001">
        <property type="entry name" value="Nicotinate-nucleotide diphosphorylase (Carboxylating)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000030">
        <property type="entry name" value="Nicotinate-nucleotide pyrophosphorylase, carboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.20">
        <property type="entry name" value="Quinolinate phosphoribosyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004393">
        <property type="entry name" value="NadC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027277">
        <property type="entry name" value="NadC/ModD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00078">
        <property type="entry name" value="nadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE [CARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179:SF3">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE [CARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006250">
        <property type="entry name" value="NadC_ModD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54675">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 9" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase N-terminal" evidence="11">
        <location>
            <begin position="19"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase C-terminal" evidence="10">
        <location>
            <begin position="104"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR006250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="277" mass="30337" checksum="C6ED82A20E8EFE80" modified="2017-09-27" version="1">MVPLDYLLRFIEEDAPFGDVTSEAVIPEGTKAKAVIIAKQEGVIAGVEEAKALFEHFGVKVEVRKRDGEEVGKGDVILNLEGDARAILLVERTALNVMGRMSGIATEVRRLVERVRAVNPKVRIAGTRKTLLKPIDKRALLIGGGEPHRFSLSDAILIKDNHLALVPLEEAIRRAKEFSVYKVVEVEVESLEDAVEAAKAGADVVMLDNMTPEEIGEAIEALKREGLRDRIKIEVSGGITPENIAEYAKLDIDVISLGYLTHSVRNFDVSLEIIGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6N1</accession>
    <name>A0A218P6N1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Dolichol-phosphate mannosyltransferase</fullName>
            <ecNumber evidence="8">2.4.1.83</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Dolichol-phosphate mannose synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Dolichyl-phosphate beta-D-mannosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Mannose-P-dolichol synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">A3L08_03440</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25 26">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O-mannosylation of proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.83" evidence="8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853707.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6N1"/>
    <dbReference type="GeneID" id="33315294"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03440"/>
    <dbReference type="OrthoDB" id="11098at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004582">
        <property type="term" value="F:dolichyl-phosphate beta-D-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06442">
        <property type="entry name" value="DPM1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000119">
        <property type="entry name" value="Dolichol-phosphate mannosyltransferase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039528">
        <property type="entry name" value="DPM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007267">
        <property type="entry name" value="GtrA_DPMS_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398:SF1">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04138">
        <property type="entry name" value="GtrA_DPMS_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16" id="KW-0464">Manganese</keyword>
    <keyword evidence="15 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="11 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="234"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="299"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="23">
        <location>
            <begin position="4"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="GtrA/DPMS transmembrane" evidence="24">
        <location>
            <begin position="236"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00053724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00074878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00082336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00082614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00083744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF04138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="ASJ06448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="352" mass="40310" checksum="9D741AE3C0EAA82D" modified="2017-09-27" version="1">MKVSVIIPTYNERENLEELFERINSALRDYDYEIIVVDDDSPDRTWEFAQKLSERYPLKVIRRTDEKGLSSAVIRGFKEAEGDVFVVMDADLQHPPEKIPELVEAIESGADIAIASRYVPGGAVKNWYWYRKLISKGAIMIGRLALPKIRDVKDPVSGFFALKREVVEGTELNPIGFKILMEILIKGRYNKVVEVPFTFGLRKAGESKLSGKTMVSYLKHVYRLMRWEGELDRIIKFTLVGLSGVLVNEGFLWAFVNFLGWDKILANIPATELAILNNFTWNDLWTFKDLKRKPLLRRLLNFHIAALTGAVVQWVIYAGLVYIGLHYLISNLVGIVVSFTVRFLVNRHVTWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6R5</accession>
    <name>A0A218P6R5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Probable GTP-binding protein EngB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">engB</name>
        <name evidence="13" type="ORF">A3L08_03590</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Necessary for normal cell division and for the maintenance of normal septation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngB GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6R5"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03590"/>
    <dbReference type="OrthoDB" id="65113at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01876">
        <property type="entry name" value="YihA_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00321">
        <property type="entry name" value="GTPase_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030393">
        <property type="entry name" value="G_ENGB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019987">
        <property type="entry name" value="GTP-bd_ribosome_bio_YsxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003255">
        <property type="entry name" value="PRK04213.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11649:SF13">
        <property type="entry name" value="ENGB-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11649">
        <property type="entry name" value="MSS1/TRME-RELATED GTP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51706">
        <property type="entry name" value="G_ENGB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0717">Septation</keyword>
    <feature type="domain" description="EngB-type G" evidence="12">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="181"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="204" mass="23234" checksum="D1FFF95CD7DA86FF" modified="2017-09-27" version="1">MIIFAGRSNVGKSTLIFRLTGKWVKRGKRPGVTRKPTEVNWRGKIIVDMPGFGFMSGVPKRVQEKVKDEIVRFIEEKADEIELAVLVVDGKAAPEIIERWEKRGEIPIDVEFYQFLRELNIPTIVAVNKLDKIKNLQRTINFLAEKFGVPPSEINETFVPISAKFGKNLDKLRLTMEKKLKEGREKPSENLKDDVGDGLLDSVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6U8</accession>
    <name>A0A218P6U8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2-haloalkanoic acid dehalogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_03735</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853757.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6U8"/>
    <dbReference type="GeneID" id="33315352"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03735"/>
    <dbReference type="OrthoDB" id="27736at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.400">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051540">
        <property type="entry name" value="S-2-haloacid_dehalogenase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43316:SF3">
        <property type="entry name" value="HALOACID DEHALOGENASE, TYPE II (AFU_ORTHOLOGUE AFUA_2G07750)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43316">
        <property type="entry name" value="HYDROLASE, HALOACID DELAHOGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="234" mass="26154" checksum="46A5C5B9B93EB909" modified="2017-09-27" version="1">MKLVSFDVWNTLLDINVMLDAMAVELSKLMGACIVDVVEGMMLTRERIKAMRAKTAGDPTRALEESQEMLAELLGIDVELVKRAAARAVLGVGDEIVLPGAKEALEGVKRRGLKVTVTGNVMFWPGSYTRLLLERFRLMDYIDKTFFADEVRAFKPMREMFEKPLKAFNVKPEEAIHIGDTKAEDFEGALKAGMWAVWINPEAEGVRQIHERGFEVPGVEGILEVLELTKGNDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Y1</accession>
    <name>A0A218P6Y1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methylmalonyl-CoA carboxyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_03915</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AccD/PCCB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853792.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Y1"/>
    <dbReference type="GeneID" id="33315388"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03915"/>
    <dbReference type="OrthoDB" id="30579at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004658">
        <property type="term" value="F:propionyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000017">
        <property type="entry name" value="Propionyl-CoA carboxylase subunit beta 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000016">
        <property type="entry name" value="Propionyl-CoA carboxylase, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051047">
        <property type="entry name" value="AccD/PCCB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43842">
        <property type="entry name" value="PROPIONYL-COA CARBOXYLASE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43842:SF2">
        <property type="entry name" value="PROPIONYL-COA CARBOXYLASE BETA CHAIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="2">
        <location>
            <begin position="3"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="3">
        <location>
            <begin position="264"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="522" mass="56939" checksum="CCE5E292ADE46A53" modified="2017-09-27" version="1">MSMEEKVNELYERKRKVLEMGGETAVEKQHAKGKLTARERIEKLLDPGSFVEIGAFVKHRGTEFGLDKKELPADGVITGYGTIDGRLVFVYAQDFTVMGGSLGEMHAAKIKRVMELALEAGAPVIGLNDSGGARIQEGVDSLKGYGEIFKMNTLLSGVVPQITAIMGPCAGGAVYSPAIGDFILMVDNPASFMFITGPQVVKAVTGVEVTPIQLGGAMVHAQRSGQAHLIGKSDEEVLALIRRLLSYLPSNNMEKPPRVKTNDLPFRKTENLYSIVPDDPNKGYDVRQVIYEIVDRDENGNPDFLEILPYFAPNAVVGFGRMNGQTVGIVANNPIHFAGVLDIDSSDKIARFVRTCDAFNIPIVTLVDVPGYLPGTQQEYGGIIRHGAKVLYAYAEATVPMVTVILRKAYGGAYLAMGSKHLGADFVYAWPTAEIAVMGPEGAANIIFRKEITQAENPEEVRQQKIKEYRDKFANPYVAAARGYIDDVIDPAETRAKIIMSLEALESKRVKLPPKKHGNIPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Y5</accession>
    <name>A0A218P6Y5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03945</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853797.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Y5"/>
    <dbReference type="GeneID" id="33315394"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03945"/>
    <dbReference type="OrthoDB" id="91317at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="196" mass="22158" checksum="D744BB954C48A73A" modified="2017-09-27" version="1">MDELEFCIKSLSYPIGMLLEKLEKKPGEFIHVVGGKITLPEVPFAALCYLTGIALFDSLDMVDKKRLSGDYDSIVAFGRKLLDSKSAEGLRTYLKSPGRYISPGERLSIDWLEFERRAERVRPYLRRVVEVQGKGALQREFLEKAAFLSELTVDEGLLLGYIAEDEKLRGLINAALGRHNPEFKAAVLRYFKALRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Z5</accession>
    <name>A0A218P6Z5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Homoserine dehydrogenase</fullName>
            <ecNumber evidence="4">1.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">A3L08_03890</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the homoserine dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.3" evidence="4"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853788.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Z5"/>
    <dbReference type="GeneID" id="33315383"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03890"/>
    <dbReference type="OrthoDB" id="4488at2157"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00063"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00465"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004412">
        <property type="term" value="F:homoserine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000005">
        <property type="entry name" value="Homoserine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000554">
        <property type="entry name" value="Homoserine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001342">
        <property type="entry name" value="HDH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019811">
        <property type="entry name" value="HDH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022697">
        <property type="entry name" value="HDH_short"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004976">
        <property type="entry name" value="PRK06349.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006235">
        <property type="entry name" value="PRK08374.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43331">
        <property type="entry name" value="HOMOSERINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43331:SF1">
        <property type="entry name" value="HOMOSERINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00742">
        <property type="entry name" value="Homoserine_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036497">
        <property type="entry name" value="HDH_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01042">
        <property type="entry name" value="HOMOSER_DHGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Aspartate/homoserine dehydrogenase NAD-binding" evidence="13">
        <location>
            <begin position="10"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Homoserine dehydrogenase catalytic" evidence="12">
        <location>
            <begin position="156"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>L-homoserine</name>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ06530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="336" mass="36573" checksum="546DBAC8DB4ABF75" modified="2017-09-27" version="1">MREVKVSLFGFGNVGRAVARVLVEKGKLFRENYGMDFKVVSISDTSGTLWLPEGVDLREALLVKETFGKLSSWTNDYEVYNLTPEEAVKEIDADIVIDVTNVENAHEWHLTALREGKAVITSNKPPLAFHYAKLIGEAERRNVPYLFEATVMAGTPIIGLLRENFLGDTVERIDAVLNATTTFILSRMEMGLHFEEALRQAQSLGIAERDPSGDILGIDAGYKATILHCVAFGPKTFDDARVKGIAEITLGEVQRARKKGKTIRLVATVERGNITVEPKEVPLDSPLSVESHENAAVIWTDLLGELVIKGAGAGLKETASGVVSDIVKAALTITGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P710</accession>
    <name>A0A218P710_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">Large ribosomal subunit protein uL18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rpl18</name>
        <name evidence="9" type="ORF">A3L08_04145</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">This is one of the proteins that bind and probably mediate the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7 8">Part of the 50S ribosomal subunit. Contacts the 5S and 23S rRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853833.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P710"/>
    <dbReference type="GeneID" id="33315433"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04145"/>
    <dbReference type="OrthoDB" id="8644at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00432">
        <property type="entry name" value="Ribosomal_L18_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.100:FF:000008">
        <property type="entry name" value="50S ribosomal protein L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01337_A">
        <property type="entry name" value="Ribosomal_uL18_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057267">
        <property type="entry name" value="Rbsml_uL18_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005485">
        <property type="entry name" value="Rbsml_uL18_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057268">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006342">
        <property type="entry name" value="PRK08569.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410:SF12">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410">
        <property type="entry name" value="RIBOSOMAL PROTEIN L5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17144">
        <property type="entry name" value="Ribosomal_L5e"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00058">
        <property type="entry name" value="RIBOSOMALL5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 8" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="201" mass="22747" checksum="0269C132C29AED92" modified="2017-09-27" version="1">MAHGPRYRVPFRRRREGKTNYHKRLALLKSGKPRLVVRKTLNHHIAQIVVYDPKGDRTLVSAHTRELMRDFGWKGHGGNTPSAYLLGLLIGYKAKKAGIEEAILDIGLHPPTKGSSIFAVLKGAVDAGLNVPHSEEIYPEDYRINGEHVANYAKALKEEDESLYRKQFGGYLVRGLEPEKLPEHFEEVKAKIIEKFEGARE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P758</accession>
    <name>A0A218P758_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Uridine phosphorylase</fullName>
            <ecNumber evidence="4 10">2.4.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">A3L08_04370</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>uridine + phosphate = alpha-D-ribose 1-phosphate + uracil</text>
            <dbReference type="Rhea" id="RHEA:24388"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; uracil from uridine (phosphorylase route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the PNP/UDP phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.3" evidence="4 10"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853868.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P758"/>
    <dbReference type="GeneID" id="33315478"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04370"/>
    <dbReference type="OrthoDB" id="372263at2157"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00633"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004850">
        <property type="term" value="F:uridine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009164">
        <property type="term" value="P:nucleoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009166">
        <property type="term" value="P:nucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd17767">
        <property type="entry name" value="UP_EcUdp-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018016">
        <property type="entry name" value="Nucleoside_phosphorylase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010058">
        <property type="entry name" value="Uridine_phosphorylase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01718">
        <property type="entry name" value="Uridine-psphlse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43691">
        <property type="entry name" value="URIDINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43691:SF13">
        <property type="entry name" value="URIDINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01232">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="11">
        <location>
            <begin position="30"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="277" mass="30057" checksum="E19FA1B4975F94FA" modified="2017-09-27" version="1">MGEKFVSAERPQTEEGYQYHIACKPGDVARYVLLPGDPERVPKISSLWDEAKEIAFHREYRTHTGKYKGVPISVTSTGIGGPSTAIAVEELAAIGADTFIRVGSTGAIQPGMEIGDLIIAKAAVRLEGTSKQYVRVEYPAVADLEVTMALIEAAESLGVRYHLGITASTDSFYLGQGRPGLNGYFPSFARNILDDLRQANVTNFEMEAATLYTLASIYGLRAGCVCAVFANRVTNEFGKAGEKEAALVASEAVKILAEWDEEKERKGKKVWFPGLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P775</accession>
    <name>A0A218P775_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribonuclease P protein component 3</fullName>
            <shortName evidence="7">RNase P component 3</shortName>
            <ecNumber evidence="7">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Rpp30</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rnp3</name>
        <name evidence="8" type="ORF">A3L08_04465</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the eukaryotic/archaeal RNase P protein component 3 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="7"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853883.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P775"/>
    <dbReference type="GeneID" id="33315497"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04465"/>
    <dbReference type="OrthoDB" id="85765at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00756">
        <property type="entry name" value="RNase_P_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023539">
        <property type="entry name" value="RNase_P_comp-3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002738">
        <property type="entry name" value="RNase_P_p30"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003023">
        <property type="entry name" value="PRK03892.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01876">
        <property type="entry name" value="RNase_P_p30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="228" mass="26092" checksum="EDCE8BEFFD24178D" modified="2017-09-27" version="1">MSAELEIREAEELSFSRDYFVEMDVRSEEAYQLASEWFDEVVFTKKLVLESSPDWGVLKEELKALRETHSKVALLLVTKKPSLIREVKNRSPRALIYVQGGDMRVNRAALEAGVDALIGPWFGRKDPGFDHILAGIAARRGVAIGFSLSPLLRANSYERVQLLRFMTKTWQLVRKYDVPRFITSSAESRWEVRGPRDLMSLGINLGMEIPEARTSLNFHPRRLLSKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P786</accession>
    <name>A0A218P786_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transcription termination factor FttA</fullName>
            <ecNumber evidence="12">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">fttA</name>
        <name evidence="16" type="ORF">A3L08_04540</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Terminates transcription on the whole genome. Termination is linked to FttA-mediated RNA cleavage and does not require NTP hydrolysis. Cleaves endonucleolytically at the RNA exit channel of RNA polymerase (RNAP); the 5'-3' exonuclease activity of this protein degrades the nascent RNA released from RNAP.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 2 Zn(2+) ions, which are required for nuclease activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer. Interacts with RNA polymerase (RNAP), interacts with the Spt4-Spt5 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. FttA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="12"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853897.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P786"/>
    <dbReference type="GeneID" id="33315512"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04540"/>
    <dbReference type="OrthoDB" id="7155at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004532">
        <property type="term" value="F:RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22532">
        <property type="entry name" value="KH-II_CPSF_arch_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02410">
        <property type="entry name" value="KH-II_CPSF_arch_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16295">
        <property type="entry name" value="TTHA0252-CPSF-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00870">
        <property type="entry name" value="FttA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019975">
        <property type="entry name" value="aCPSF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050698">
        <property type="entry name" value="MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033769">
        <property type="entry name" value="TffA_KH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03675">
        <property type="entry name" value="arCOG00543"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203:SF51">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17214">
        <property type="entry name" value="KH_TffA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 12" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 12" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 12" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="K Homology" evidence="13">
        <location>
            <begin position="102"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="14">
        <location>
            <begin position="198"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-Casp" evidence="15">
        <location>
            <begin position="433"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="KHa" evidence="12">
        <location>
            <begin position="9"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="KHb" evidence="12">
        <location>
            <begin position="77"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Beta-Casp" evidence="12">
        <location>
            <begin position="396"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Metallo-beta-lactamase C-terminus" evidence="12">
        <location>
            <begin position="590"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="615"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ASJ06641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="648" mass="73539" checksum="686810082E51E49E" modified="2017-09-27" version="1">MIRRETFVDDILRDIREVISQMVPPEARITEVEFEGPELVIYVKNPEAIMRDGELIKNLAKVLKKRISVRPDPDILLPPEKAEEMIKELVPKEAEITNISFDPSVGEVIIEAKKPGLVIGKNGETLRLITQKVHWAPRVIRTPPLQSQTIYSIRQILQAEAKDRRKFLRQAGRNIYRKPETKSEWIRITGLGGFREVGRSALLVQTNESYVLVDFGVNIAALRDPKKAFPHFDAPEFRYVLDAGLLDAIIITHAHLDHSGMLPYLFRYKLFDGPIYTTPPTRDLMVLLQQDFIEIQKMNGVEPLYRPRDIKEVIKHTITLDYGEVRDIAPDMRLTLHNAGHILGSSIVHLHIGNGLHNIAITGDFKFIPTRLFEPAVSRFPRVETLVMESTYGGSNDYQMPREEAEKRLIEVIHQTIRRGGKVLIPAMAVGRAQEIMMVLEEYARIGGLEVSIYLDGMIWEATAIHTAYPEYLSRHLREQIFHEGYNPFLNPIFKSVANSRERQDIIDSGEPAIIIATSGMLVGGPSVEYFKQLASDPKNSIIFVSYQAEGTLGRQVQRGLREIPLVGEDGRTEVVNVNMEVHTIDGFSGHADRKELISYIARLRPRPERVITVHGETHKCLDLSTSIHKKFGIPTRAPNNLDAIRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P791</accession>
    <name>A0A218P791_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_04605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853908.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P791"/>
    <dbReference type="GeneID" id="33315525"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04605"/>
    <dbReference type="OrthoDB" id="17914at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33594:SF1">
        <property type="entry name" value="HD_PDEASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33594">
        <property type="entry name" value="SUPERFAMILY HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G03035)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="34"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="206" mass="23528" checksum="0E93483848C59FAD" modified="2017-09-27" version="1">MRLADFISDPKSIELIERTREYAKHFFEREGTHGFSHVERVFNLCMHIGREESADLEVLALAALLHDVARPLESAGKVEDHAAEGARIARHFLRSIGYPKEKVEAVAHAIEAHRFSRGPEPETLEAKILSDADKLDAIGAIGIARVFMYSGEHGRSIEDSLAHFEEKILKLKDLMYTQTAKRLAEERHRFTVEFIERIRREIEGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7B9</accession>
    <name>A0A218P7B9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA topoisomerase 1</fullName>
            <ecNumber evidence="11">5.6.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">DNA topoisomerase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">topA</name>
        <name evidence="15" type="ORF">A3L08_04755</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853936.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7B9"/>
    <dbReference type="GeneID" id="33315555"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04755"/>
    <dbReference type="OrthoDB" id="30963at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03362">
        <property type="entry name" value="TOPRIM_TopoIA_TopoIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.460.10:FF:000018">
        <property type="entry name" value="DNA topoisomerase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.140:FF:000006">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.65.10">
        <property type="entry name" value="Bacterial Topoisomerase I, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="entry name" value="Topoisomerase I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.20.10">
        <property type="entry name" value="Topoisomerase I, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="entry name" value="Topoisomerase I, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00952">
        <property type="entry name" value="Topoisom_1_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000380">
        <property type="entry name" value="Topo_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023406">
        <property type="entry name" value="Topo_IA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013825">
        <property type="entry name" value="Topo_IA_cen_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013498">
        <property type="entry name" value="Topo_IA_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005739">
        <property type="entry name" value="TopoI_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028612">
        <property type="entry name" value="Topoisom_1_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034144">
        <property type="entry name" value="TOPRIM_TopoIII"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004438">
        <property type="entry name" value="PRK05776.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01057">
        <property type="entry name" value="topA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390:SF26">
        <property type="entry name" value="DNA TOPOISOMERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390">
        <property type="entry name" value="PROKARYOTIC DNA TOPOISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01396">
        <property type="entry name" value="Zn_ribbon_Top1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="Prokaryotic type I DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00396">
        <property type="entry name" value="TOPO_IA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52039">
        <property type="entry name" value="TOPO_IA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 11 15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 11" id="KW-0799">Topoisomerase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Toprim" evidence="13">
        <location>
            <begin position="2"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IA-type catalytic" evidence="14">
        <location>
            <begin position="157"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with DNA" evidence="11">
        <location>
            <begin position="197"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="360"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="594"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="360"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="11">
        <location>
            <position position="318"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="11">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="11">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="11">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="11">
        <location>
            <position position="320"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="11">
        <location>
            <position position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS52039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ASJ06679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="728" mass="82048" checksum="80DCCD441EB9DF67" modified="2017-09-27" version="1">MVTLIIAEKPNVARKIAYALAEGKPVRKTIGKVSYYEFTRDGKRVIVAPAVGHLFSLAPKVKTYGYPIFDIEWVPVYVAEKGKSYAKDYIKALATLAKQADEFVVACDYDTEGEVIGYTALKYACGVDPSKAKRMKFSALTKKDLLKAWYNLEPTINFGMADAGVARHVLDWYWGVNLSRALTSAIKRASGKWMVLSTGRVQGPTLKFLVDREKEIQNFKPTPYWVIKMLLEKNGEQYTATYEKERILDEEEAKRIVEEAKKGPAFVEKVEVKQQNRNPPAPFDLGTLQREAYSAFGYSPKKTLEIAQKLYEKSLTSYPRTSSQKLPKNLNFRAILQNLAKLPEYKPFAHALLGKENLKPVEGKKDDPAHPAIYPTGELPKPGDLTKDEQNIYDLVVRRFLALFMEPAVRETMKVIINSNNHRFILSGARTVKEGWLKVYGKYVKFDEVILPAFKEGEPVKVLQIKREKKKTKPPARYSPAAVIKKMEDLGIGTKATRAQILETLYSRGYIEGKRKIKVTPLGMRVVEALEKNVPDIVSVELTRAFEEKMEDIMAGKAEKEQVIEESKNQLVKILKVFKEKEMDIGKMLLESTGTGVTPSKSSARKGGAVKELSEEEEREVQRAVDGKASSENAESKKPAKKALVIGKCPKCGGDLVVRYNRKTGKRFVGCSNWPKCDVTYPLLQRGEIIPTDKTCCNGAPVVKIREGKKEYEICIDMNCRDWKKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7C5</accession>
    <name>A0A218P7C5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Transcription elongation factor Spt5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nusG</name>
        <name evidence="4" type="synonym">spt5</name>
        <name evidence="8" type="ORF">A3L08_04810</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Stimulates transcription elongation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterodimer composed of Spt4 and Spt5. Interacts with RNA polymerase (RNAP).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPT5 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the archaeal Spt5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853942.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7C5"/>
    <dbReference type="GeneID" id="33315566"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04810"/>
    <dbReference type="OrthoDB" id="371863at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06091">
        <property type="entry name" value="KOW_NusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09887">
        <property type="entry name" value="NGN_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.940">
        <property type="entry name" value="NusG, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00950">
        <property type="entry name" value="Spt5_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005100">
        <property type="entry name" value="NGN-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006645">
        <property type="entry name" value="NGN-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036735">
        <property type="entry name" value="NGN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039659">
        <property type="entry name" value="SPT5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011590">
        <property type="entry name" value="Spt5_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00405">
        <property type="entry name" value="KOW_elon_Spt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11125">
        <property type="entry name" value="SUPPRESSOR OF TY 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11125:SF7">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03439">
        <property type="entry name" value="Spt5-NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00738">
        <property type="entry name" value="NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NusG-like N-terminal" evidence="6">
        <location>
            <begin position="3"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="7">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="NCBIfam" id="TIGR00405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="152" mass="16754" checksum="457C18E0AE65045E" modified="2017-09-27" version="1">MSDGKIFTVRVTVGQEETTAKLIYSKVKTYNLPVYAILTPSKVKGYIFVEAPNKSAVDEAIKGIRHAKGTLPGEVKFEEIEHFLEEKPAVSGFEPGDIVELIAGPFKGEKAKVVRVDEGKDEIVVELVGSIVPIPVTVRGEYVRLISKRQKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7C9</accession>
    <name>A0A218P7C9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_04745</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853935.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7C9"/>
    <dbReference type="GeneID" id="33315553"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04745"/>
    <dbReference type="OrthoDB" id="15347at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050572">
        <property type="entry name" value="Fe-S_Ferredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687:SF5">
        <property type="entry name" value="4FE-4S FERREDOXIN-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687">
        <property type="entry name" value="ADENYLYLSULFATE REDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14697">
        <property type="entry name" value="Fer4_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="5"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="67" mass="7225" checksum="70F24719C4F1AD78" modified="2017-09-27" version="1">MPEKIRVVVNEDRCYLCGGCAGVCPALAIEVHSSGWEFLQDKCISCRICINACPVGALSAEPLEVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7E1</accession>
    <name>A0A218P7E1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fucose isomerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_04775</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853937.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7E1"/>
    <dbReference type="GeneID" id="33315559"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04775"/>
    <dbReference type="OrthoDB" id="26618at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016861">
        <property type="term" value="F:intramolecular oxidoreductase activity, interconverting aldoses and ketoses"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005996">
        <property type="term" value="P:monosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009015">
        <property type="entry name" value="Fucose_isomerase_N/cen_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36120">
        <property type="entry name" value="FUCOSE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36120:SF2">
        <property type="entry name" value="FUCOSE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53743">
        <property type="entry name" value="FucI/AraA N-terminal and middle domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="402" mass="44959" checksum="A0C061E968D6A7CC" modified="2017-09-27" version="1">MKVGVVFGVSELANPKAFEKKASEFITNLAKDFEVVGGFFVSTKRDFKEAKEEVDFNEVDAIVLYPLTGGTEGPLKEFSVYRRPIVIYGDPFNNSIAAGIELREYFRERLVPSTLVKDLNELKAALLGYEDMKETLDKFLRMRIGLIGRVSPWLINERFELPYTSISLKKFYEYYDAVTDEEGWSAVEDIVSKAVEIKEPDREALTKAGRIYVAIKRILDDYKLDGFTIGCFDLIGKIGSTPCLALAMFNAQGIPAACEGELNSLVGMMIARRFFDKPAFMGNIADYGDDYLILAHCTAPLIGKYIIRSHFESGMGVGVDVQLPKGKASLLKIRGRKAVVAGVEVVGQEHSNMRCRTQVKLRIEDAMDFIDGTLGNHHLLIYVDSEELADLLSELGFEVMLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7H3</accession>
    <name>A0A218P7H3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rps7</name>
        <name evidence="9" type="ORF">A3L08_05065</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853983.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7H3"/>
    <dbReference type="GeneID" id="33315617"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05065"/>
    <dbReference type="OrthoDB" id="45346at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="entry name" value="Ribosomal protein S7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00480_A">
        <property type="entry name" value="Ribosomal_uS7_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_uS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026018">
        <property type="entry name" value="Ribosomal_uS7_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_uS7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_uS7_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005716">
        <property type="entry name" value="Ribosomal_uS7_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003106">
        <property type="entry name" value="PRK04027.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01028">
        <property type="entry name" value="uS7_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="Ribosomal protein S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS7" evidence="8">
        <location>
            <begin position="34"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="215" mass="24391" checksum="88756E4533487697" modified="2017-09-27" version="1">MAKALTERFFVPKEPKVMGRWSVEEVTVNDPSLRPYINLEPRILPHSHGRHAKKSFGKANVHIVERLINKVMRSGASHSKVGGHFMRREHRSLMSKKMKAYEVVKEALMIIERRTKQNPIQVLVRAIENSAPREDTTTIAFGGIRYHMSVDVSPLRRLDIALKNIALGASAKCYRNKTSYAQALAEEIIAAANKDPKSFAYSKKEEIERVAQSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7I2</accession>
    <name>A0A218P7I2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cobalt transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_05160</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853997.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7I2"/>
    <dbReference type="GeneID" id="33315636"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05160"/>
    <dbReference type="OrthoDB" id="103658at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025937">
        <property type="entry name" value="PDGLE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13190">
        <property type="entry name" value="PDGLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="PDGLE" evidence="7">
        <location>
            <begin position="3"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="82" mass="8638" checksum="2696141ED0E6475D" modified="2017-09-27" version="1">MRTVFKGLIIIALLLAIVLPLASSNPDGLEATMEKVGLEEKPVYQAPLDYGETWGQSFAMGLLGITLAFGVGYGLAKLARGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7J0</accession>
    <name>A0A218P7J0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transcription elongation factor NusA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854005.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7J0"/>
    <dbReference type="GeneID" id="33315644"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05200"/>
    <dbReference type="OrthoDB" id="84871at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="59" mass="6550" checksum="A817F05DBB059812" modified="2017-09-27" version="1">MKDRLEKMLNVKILEMEELEDKIIVYVPKDQVRIAVGSGGAAVKAAELVIGRKIEVVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7K2</accession>
    <name>A0A218P7K2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SUF system FeS cluster assembly SufBD core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_05270</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron-sulfur cluster assembly SufBD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7K2"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05270"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055346">
        <property type="entry name" value="Fe-S_cluster_assembly_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508">
        <property type="entry name" value="FES CLUSTER ASSEMBLY PROTEIN SUF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508:SF1">
        <property type="entry name" value="UPF0051 PROTEIN ABCI8, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="Stabilizer of iron transporter SufD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD core" evidence="2">
        <location>
            <begin position="11"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00043967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="96" mass="10660" checksum="6D87B08F90510048" modified="2017-09-27" version="1">MQTSSDFILSLVNSDQAEQDVQTVPLLRVKNDKAELTHEAPIGRVNEAQLVQLMAKGLAKEEATELIIKGLLGSELRPDKKEKLVKQGVLQRFHPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7L3</accession>
    <name>A0A218P7L3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleotidyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854026.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7L3"/>
    <dbReference type="GeneID" id="33315666"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05310"/>
    <dbReference type="OrthoDB" id="9287at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05403">
        <property type="entry name" value="NT_KNTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052548">
        <property type="entry name" value="Type_VII_TA_antitoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33933">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33933:SF3">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE MJ0604-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="106" mass="12407" checksum="8871278B06B17CCA" modified="2017-09-27" version="1">MKEKELIKKTVVEVCRELGIELGEVILFGSRARGDFRKDSDWDILLVTERELSWRERLRLSGEIRKRLAKKGMPIDVLIISREKLERLKNDPGYVYSYALSEGIRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7L4</accession>
    <name>A0A218P7L4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Hydrogenase 3 maturation endopeptidase HyCI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854028.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7L4"/>
    <dbReference type="GeneID" id="33315668"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05320"/>
    <dbReference type="OrthoDB" id="44145at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06067">
        <property type="entry name" value="H2MP_MemB-H2evol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1450">
        <property type="entry name" value="HybD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004420">
        <property type="entry name" value="Pept_A31_hyd_mat_HycI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023430">
        <property type="entry name" value="Pept_HybD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000671">
        <property type="entry name" value="Peptidase_A31"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00072">
        <property type="entry name" value="hydrog_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30302:SF7">
        <property type="entry name" value="F420-NONREDUCING HYDROGENASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30302">
        <property type="entry name" value="HYDROGENASE 1 MATURATION PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01750">
        <property type="entry name" value="HycI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00446">
        <property type="entry name" value="HYDRGNUPTAKE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53163">
        <property type="entry name" value="HybD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="161" mass="17498" checksum="5673DC6492BDD268" modified="2017-09-27" version="1">MNALEEIFAGKKRIVICGIGNDIRGDDAFGVLVAERLKELLDSPNVLVLNCGEVPESYVGKITSFKPDLVVFVDAVDFEGEHGELIITDPEGTVGEAVSTHSLPLRVFVKYLKTQIKADFVLMGCQPASTGLFEEPSELIKEMAERLAELLAGILRGDGYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7N0</accession>
    <name>A0A218P7N0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">dolichyl-phosphooligosaccharide-protein glycotransferase</fullName>
            <ecNumber evidence="6">2.4.99.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Oligosaccharyl transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">A3L08_05410</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>an archaeal dolichyl phosphooligosaccharide + [protein]-L-asparagine = an archaeal dolichyl phosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.</text>
            <dbReference type="EC" id="2.4.99.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the STT3 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.21" evidence="6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854046.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7N0"/>
    <dbReference type="GeneID" id="33315686"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05410"/>
    <dbReference type="OrthoDB" id="82393at2157"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004576">
        <property type="term" value="F:oligosaccharyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010598">
        <property type="entry name" value="C5-epim_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003674">
        <property type="entry name" value="Oligo_trans_STT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038731">
        <property type="entry name" value="RgtA/B/C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872:SF1">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT STT3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06662">
        <property type="entry name" value="C5-epim_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13231">
        <property type="entry name" value="PMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="162"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="183"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="273"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase RgtA/B/C/D-like" evidence="19">
        <location>
            <begin position="67"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="D-glucuronyl C5-epimerase C-terminal" evidence="18">
        <location>
            <begin position="694"/>
            <end position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF06662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF13231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ASJ06795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="819" mass="94910" checksum="8D1A5E8A06D1C268" modified="2017-09-27" version="1">MNKERLYLPMILILSFIVRLIPHRTLLLATYDEYLHRDITLRIASQGVGSISRDILSLFGLRAYSYPPLFHIIGAAFYRVFPSDYIFFVLPALYGTLAVLGFYLAFKELTGDRNRAILATTLLAFAPNFIYRTSLYIPENLGLFLFSLSMLFGLRLLKSREPSNFLLFGVVFLVYMVTHRGWLFFVLAAFFVMVSYWWGFIRRNLQYFLALAVIGIMVYTQVSFIQSTVGELALRLQRSEVSFLGYFKWIGVIQLVFGSLATPYYLKSDRIRRGFALWAWAFMLAGGVSFRFRDPYAAIPLSVMASEYILDTVIPRIGSLLRKTFEGVKGLGAEWIRKTSQKKWATSIVVLLLLSSPVVQGFYGAYKYVEAPTVSDREAYEWISQNTPENATILVWWDMGYLLIGNTKRKDVVIWKKVYQGFFGEAPTVKEASKAYFDHVVMFSSSQREWVYYLMKKYNVSYVFVDRKRYSYGLVRYGLMEYAAYDTHFKLEFCDGNSVIYRFIPEPALKMEQPFPIDYSGNYSPLVGFLEKFWTGYNYADFDSKYKAYFNLNAWIFDVYSRLYQKTGEEEFKERANWLLRWLYYKQMNNGAFPWGVPPNDYTLYTAYTLEPLIGSSFEGSERALDLLRERQKGDFFMTTPKDKKGSPLVNSMLLPLYREFGILNRTTERNVLEKALSEQKGDGSWNDNVGTTTAIASSLARYYQLTGNETVLNAVRKAAEWLAKQQGEDGKLEDEKYEYAFSRSTYVQMAYIYHVAGLKDAEELTLKFIHETFDPNKEASPLDSVITMYRYFSYAYGSDRALEMVNDLLKLHPLLEFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Q4</accession>
    <name>A0A218P7Q4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11 12">Uracil phosphoribosyltransferase</fullName>
            <ecNumber evidence="3 11 12">2.4.2.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">UMP pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">UPRTase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">upp</name>
        <name evidence="14" type="ORF">A3L08_05430</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>UMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + uracil</text>
            <dbReference type="Rhea" id="RHEA:13017"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 1 Mg(2+) ion per subunit. The magnesium is bound as Mg-PRPP.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="11">Allosterically activated by GTP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the UPRTase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.9" evidence="3 11 12"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854050.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Q4"/>
    <dbReference type="GeneID" id="33315690"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05430"/>
    <dbReference type="OrthoDB" id="80352at2157"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00636"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004845">
        <property type="term" value="F:uracil phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006223">
        <property type="term" value="P:uracil salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01218_A">
        <property type="entry name" value="Upp_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034331">
        <property type="entry name" value="Upp_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005765">
        <property type="entry name" value="UPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050054">
        <property type="entry name" value="UPRTase/APRTase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001097">
        <property type="entry name" value="PRK00129.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01091">
        <property type="entry name" value="upp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32315">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32315:SF4">
        <property type="entry name" value="URACIL PHOSPHORIBOSYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="5 11 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="13">
        <location>
            <begin position="15"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="38"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="140"/>
            <end position="148"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>uracil</name>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="209"/>
            <end position="211"/>
        </location>
        <ligand>
            <name>uracil</name>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="NCBIfam" id="TIGR01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ06799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="222" mass="24801" checksum="ADB903348CDEE367" modified="2017-09-27" version="1">MKRDERWEGVYSFEDSPFIMEILTELRDERTGPIAFRKGLVKLGRYMAYELTKTMEVEKVPVKTPLEETEGVLVKDRRNVVIITVLRAAIPLMEGLIKVLDHARVGIVSASRGKAPKFEIEMNYVKIPEIKPEDTVIVADPMIATGSTLIKVLEEVKRYGRAKRYVVVGVLAAPEGISRIKEAHPDVQIFVAAIDRELNEKGYILPGLGDAGDRAFGAPVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7T4</accession>
    <name>A0A218P7T4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_05710</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854101.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7T4"/>
    <dbReference type="GeneID" id="33315746"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05710"/>
    <dbReference type="OrthoDB" id="30924at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038767">
        <property type="entry name" value="PF0610-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049159">
        <property type="entry name" value="PF0610-like_wHTH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057022">
        <property type="entry name" value="PF0610-like_Zn_ribbon_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40663">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40663:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21476">
        <property type="entry name" value="PF0610-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23470">
        <property type="entry name" value="Zn_ribbon_PF0610"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PF0610-like winged HTH N-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="PF0610-like rubredoxin-like zinc beta-ribbon C-terminal" evidence="2">
        <location>
            <begin position="59"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="97" mass="11348" checksum="57C3C295478D31AB" modified="2017-09-27" version="1">MMTRRQRIIKLLEERDYSPSELALALDMRGKGIKKVILEDLKAISKTLKHEGKVLLIKPAECRKCGFVFKPEIKLPSKCPRCHSSWIEEPRFKIEIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7U3</accession>
    <name>A0A218P7U3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">TIGR00374 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_05780</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0104 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854115.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7U3"/>
    <dbReference type="GeneID" id="33315760"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05780"/>
    <dbReference type="OrthoDB" id="15513at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022791">
        <property type="entry name" value="L-PG_synthase/AglD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00374">
        <property type="entry name" value="flippase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39087">
        <property type="entry name" value="UPF0104 MEMBRANE PROTEIN MJ1595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39087:SF2">
        <property type="entry name" value="UPF0104 MEMBRANE PROTEIN MJ1595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03706">
        <property type="entry name" value="LPG_synthase_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="176"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="293" mass="32120" checksum="F2604A059E3A4109" modified="2017-09-27" version="1">MLESLASSMKQYFSIVSSASLKYLALAFLTYYVSVVIYGIRWKLVLNGIGRDAPLKELVKAILASIFMNNVTPLSRSGGELLRMAWVSKRAHIPVGVSAVSIVYERTLETIPVFALFLVGMSYFSSGEPLPLVLLGIAGIALIWIKWDSFVRLSLRLFRTQVTREEMEKITSLRKMHNLNMIAILLSSSIWLLDVTRLKLITMAFGLNLPWSLIAVISIANLLFGLIAFTPGGIGIIEGGLIGTLTYFGIPMAIALSVTLLERFVSYVASSLVGLAVLLTSGGVEIWKALKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7V6</accession>
    <name>A0A218P7V6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">tRNA pseudouridine synthase Pus10</fullName>
            <ecNumber evidence="8">5.4.99.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">tRNA pseudouridine 54/55 synthase</fullName>
            <shortName evidence="8">Psi54/55 synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pus10</name>
        <name evidence="11" type="ORF">A3L08_05715</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Responsible for synthesis of pseudouridine from uracil-54 and uracil-55 in the psi GC loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>uridine(54) in tRNA = pseudouridine(54) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:57876"/>
            <dbReference type="Rhea" id="RHEA-COMP:10193"/>
            <dbReference type="Rhea" id="RHEA-COMP:14141"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>uridine(55) in tRNA = pseudouridine(55) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42532"/>
            <dbReference type="Rhea" id="RHEA-COMP:10101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10102"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the pseudouridine synthase Pus10 family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854102.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7V6"/>
    <dbReference type="GeneID" id="33315747"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05715"/>
    <dbReference type="OrthoDB" id="10348at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160148">
        <property type="term" value="F:tRNA pseudouridine(55) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2510:FF:000001">
        <property type="entry name" value="tRNA pseudouridine synthase Pus10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3190:FF:000001">
        <property type="entry name" value="tRNA pseudouridine synthase Pus10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01893">
        <property type="entry name" value="Pus10_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005912">
        <property type="entry name" value="Pus10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039894">
        <property type="entry name" value="Pus10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048741">
        <property type="entry name" value="Pus10-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055174">
        <property type="entry name" value="Pus10_THUMP_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01213">
        <property type="entry name" value="pseudo_Pus10arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21568">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE PUS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21568:SF0">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE PUS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21238">
        <property type="entry name" value="Pus10_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22023">
        <property type="entry name" value="Pus10_THUMP_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 8" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="THUMP" evidence="10">
        <location>
            <begin position="37"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="386" mass="43679" checksum="5892DA971A3544D2" modified="2017-09-27" version="1">MITEKAGRVLEKYELCDHCLGRLFARLGKGTNEERGRAIRFVLNMERAAAGLPPITEPKKCGLCGNVFERIPELVERMKEASAGIEFDTFLVGSRFPAEVREKEEVIWREFGIETGEPINREFNRELGKAFGLATGKDTARNPDVVFIVEPYSAELELQINPIYVYGRYRKLVRGIPQTPLPDFEDSVASIICRAFSGASGGKCVFKGAGREDVDVRMLGNGRPFIVEIKRPRRRKLDLKSIAEEINASGRVEVLDLSFVAPKEAEEVLTKNHRKEYLALVLVEDGVTPEEAEEVARKLKGLEIHQRTPWRVRKVRADKVRVKRVHEAEARWLDEKHFELRLVTDGGLYIKELVSGDKGRTKPSVSDLLGKPAWCERLDVLNILDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7V9</accession>
    <name>A0A218P7V9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05845</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854128.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7V9"/>
    <dbReference type="GeneID" id="33315773"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05845"/>
    <dbReference type="OrthoDB" id="86049at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="324" mass="37533" checksum="A81FC54076D12170" modified="2017-09-27" version="1">MKVIRFGPSIVFLRTAHLEAVKEAIKDVFGVDEVDTERAIRESSEFETVLFVTDEWQKKTLPPQSGFLIKAHAPFVLSQVINNGFPVEKVHVESTLIFFRVPEKTDEALKFLAHKYGGEVMDIRNALDEGEAGDTIIGITRKKLTCPIGPEEIEGAVLVRRSFLEVYRELLLDAPLLLLKLMPEWNELTIKIYDTEKRYEENIERLMMVIEDLDLGFVVGEGWDWDYPRPFMRVPVYKLKLLTWEKPERVKFLLKGIEYVGYKRLCDIDVFVEGKKIHWTSLGKYDSKFELAKAAREELEKHLSEDVLKKLRELEEKLVAESKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7X1</accession>
    <name>A0A218P7X1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05800</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854119.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7X1"/>
    <dbReference type="GeneID" id="33315764"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05800"/>
    <dbReference type="OrthoDB" id="85216at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="156" mass="17668" checksum="7EDBCF04A6A979F1" modified="2017-09-27" version="1">MKVEGFVASLKNAETIGELFKILGEKGAPIVELDGQRFLIVVEGDFEGRKFWTEINGEKANQALGDAMLNSSSFPFKCKRPYTGGNVIFVNFDDIEVEKFLVAYRDPDYGIFYLVENGDAKEITTEEYEKLLPEMPEFKIKSMSEEQMDMMGAFFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7X2</accession>
    <name>A0A218P7X2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Agmatinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_05815</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="4">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the arginase family. Agmatinase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854122.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7X2"/>
    <dbReference type="GeneID" id="33315767"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05815"/>
    <dbReference type="OrthoDB" id="7186at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033389">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via agmatine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11593">
        <property type="entry name" value="Agmatinase-like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005925">
        <property type="entry name" value="Agmatinase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01230">
        <property type="entry name" value="agmatinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="ARGINASE/AGMATINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358:SF26">
        <property type="entry name" value="GUANIDINO ACID HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036979">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR036979-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="288" mass="31604" checksum="1E522AE0D33BBBA2" modified="2017-09-27" version="1">MDFLYTYETLKLEFPLVEPESADFIIVGVPFDGTTSFKAGARFGPTLIRHATLNLESYILDYDIDIAELPIADIGDVAVVAGDPRKTADRVRETIEEIKRANPSALPILLGGEHSQTLGAVEALKPKSYVVFDAHLDLRDSYEDNPHNHACVARRISELGVKEAMFGIRSGTKEEVEFAEKNGIRWVHARDYSFDAFVDLVSSLPDPVYLSVDIDAFDLSMVPSTGTPEAGGLAFWDVVEAIEWLAEKKRIAGFDIMEVAGEGLGDPTALTAAKLLFYFIGAVAKFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Y2</accession>
    <name>A0A218P7Y2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">histidinol-phosphate transaminase</fullName>
            <ecNumber evidence="4">2.6.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">A3L08_05895</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23744"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="2.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.9" evidence="4"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854138.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Y2"/>
    <dbReference type="GeneID" id="33315783"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05895"/>
    <dbReference type="OrthoDB" id="39225at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004400">
        <property type="term" value="F:histidinol-phosphate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050106">
        <property type="entry name" value="HistidinolP_aminotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006225">
        <property type="entry name" value="PRK08354.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43643:SF6">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43643">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 13" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="9" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="12">
        <location>
            <begin position="30"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="326" mass="37299" checksum="2DBB984F1921E410" modified="2017-09-27" version="1">MLEPVKFSTYHGGAREEGLLDFSASLNPYPPEWLDEMFERAKEISDRYPYYEKLEEGLSELIGEEVTVTAGITEALYLLGILALRGRKVVIPEHTYGEYERAARIFGAKVVKGPNGPEKLAELVERDSVVFFCNPNNPNGRFYHVRELKPLLDAVEDKNALLVLDEAFIDFVKNPESPEGENVVKLRTFTKSYSLPGIRVGYIIGFPEAFRSVRMPWSIGSTGLAFLEFLLEDGFEHLRRTMPLIWREKERIERALGVKSDANFFIKRVGDAKKVVEALKRQGILVRNCESFGLPEYIRFSVKRPEENEMLVRALKEVEDKMENGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P809</accession>
    <name>A0A218P809_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05990</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854155.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P809"/>
    <dbReference type="GeneID" id="33315802"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05990"/>
    <dbReference type="OrthoDB" id="84822at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.830">
        <property type="entry name" value="Bifunctional heparan sulphate n-deacetylase/n-sulphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="113" mass="13456" checksum="1A57B832CD4AC50A" modified="2017-09-27" version="1">MARCPLCGKSLDWGELIEQMIEEKGTEMRELLLDRERFLSAFREFVFKCPHCGEEFYGRNLPEKEAEKVFELLNDFKGSIDWENKKVRLRLNSLLALDTLLEQWDEKVTRKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P818</accession>
    <name>A0A218P818_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_06070</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RuvB family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="2"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854168.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P818"/>
    <dbReference type="GeneID" id="33315817"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06070"/>
    <dbReference type="OrthoDB" id="45425at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000010">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.360:FF:000001">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.360">
        <property type="entry name" value="RuvB-like helicase, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027238">
        <property type="entry name" value="RuvB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041048">
        <property type="entry name" value="RuvB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042487">
        <property type="entry name" value="RuvBL1/2_DNA/RNA_bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010339">
        <property type="entry name" value="TIP49_P-loop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11093">
        <property type="entry name" value="RUVB-RELATED REPTIN AND PONTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06068">
        <property type="entry name" value="TIP49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17856">
        <property type="entry name" value="TIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="62"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="440" mass="49040" checksum="21F1E89DFB49D9F9" modified="2017-09-27" version="1">MPVIEEVAKRTFERIGSHSHIKGLGLDENGKALFMADGMVGQVKAREAAGIAVELIKRGKLAGKGILLVGPTGSGKTAIAMGIARELGEDVPFVQIAGSEIYSAEINKTEFLKEALRRAIGVRISEERKVYEGEVKEVKINKTKHPFNPYVEVPESVLITLRTKDDEKTVRAGREIAYQLMELGVEEGDVIQIDAETGRISKVGTTKEEEGLFFKRKVNLPSGPVLKIKEFTYTVTLHDLDVANARGNIFGLLFSTGAEISDEIRQRVDEMVKQWIEEGKATLVPGVLFIDEVHMLDIEAFSFLARAMESELAPILILATNRGRTKIRGTDIEAPHGMPIDMLDRLLIINTEPYKKEEIREIVKIRAKEEKIEVSDEAIEYLAELGEKTSLRYAVQLLAPASVLARGGRVEREHVEKAKEYFADLRRSTEFVEKLEGMLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P822</accession>
    <name>A0A218P822_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854173.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P822"/>
    <dbReference type="GeneID" id="33315822"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06095"/>
    <dbReference type="OrthoDB" id="89081at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="150" mass="16369" checksum="C8E2BA6FE6CCDA4D" modified="2017-09-27" version="1">MKENITPAQMKWGVSLIMTLAYAFGAAYFYYLPSRGEPGLEVGFWQLLLHFLVLSFLFWLVLIPEGSSAKAADLLMLTVLTVPAGVAGALISLFAVTGIGIGETRGLEYLVMALIPYTLAYKAVLGKAGWRPAILAATYWIITYLWGSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P846</accession>
    <name>A0A218P846_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Class III signal peptide-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P846"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06325"/>
    <dbReference type="OrthoDB" id="96953at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007166">
        <property type="entry name" value="Class3_signal_pept_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04021">
        <property type="entry name" value="Class_IIIsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="17345" checksum="8A940CDE388DB4BA" modified="2017-09-27" version="1">MKKGQGSIEYLVMVTVALILLALVFHYVYTSSKGVPITGITYIDPELSPEKPGYDHPVTWVVYKYPLGCEATKNCDFYVSVNLHYYPDTGKYRFWVYANGDSADTKKIRVRLCNGATGEWNFPEDKGKNKINGVYLHEDDFPCALSIMAWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P897</accession>
    <name>A0A218P897_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hydantoin racemase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_06510</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
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    </dbReference>
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        <property type="entry name" value="CPA3_Na-H_Antiporter_A"/>
    </dbReference>
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        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005097">
        <property type="entry name" value="PRK06525.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NA(+)/H(+) ANTIPORTER SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43373:SF1">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01434">
        <property type="entry name" value="NADHDHGNASE5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="270"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="374"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="404"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="446"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="2">
        <location>
            <begin position="133"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="474" mass="51793" checksum="5D272F1F7B6B7941" modified="2017-09-27" version="1">MIDLIISSIVLAYLGVVCFVLDDRKSDRVMLPLLWLSSLLQVIVAAMFYLSPNPIHAVFLEVNGLGEVYGLRVDGTSVFTAFVVSTAGAVFLTYAVRYMDDKNVGHPHRGQKGRFYGWMMLFLGSTLAFIYSSTVLQMLIFFELMSVACWGVVGFYGSKRAERAALKALLIPNFGAIVGFYTAVAYGLKYGNLGLDFLGALPEMEKVILFLCLMVAAFTKSAQFPLYSWIPDAMEAPTPASAFLHGAAMVEMGVYLLIRVVQFMEPPVTVFYPMALILVATLIVAMLQYPRQSDAKRLLAYSTIAECAIMYTGVAIAVLGNPLGIKAALFQLMNHAYLKGLAFLTAGTFTYYYGTLDMRRIKGLKETPLLAYSWSFALLGLAGLPPFGVFFSKLYIYLNAGEMYSVPLGALLLLVVLADSVVLLYSALKSINSMVFSEGEPKRVDGIISGTLCSLILLSLISAFIGYALLGVVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8B3</accession>
    <name>A0A218P8B3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-keto acid:ferredoxin oxidoreductase subunit delta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854296.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8B3"/>
    <dbReference type="GeneID" id="33315960"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06785"/>
    <dbReference type="OrthoDB" id="51316at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050011">
        <property type="entry name" value="OGOR_delta"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF042987">
        <property type="entry name" value="OGOR_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43122">
        <property type="entry name" value="FERREDOXIN SUBUNIT OF PYRUVATE:FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43122:SF1">
        <property type="entry name" value="IRON-SULFUR-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="23"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="62"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="90" mass="10021" checksum="2CC49429A404C648" modified="2017-09-27" version="1">MADVEGKTTVEKNGYLVVGKAEGIVEIDVDTFLCKGCGICVEMCPRKVFEWSKELSEKGVHYPVPVHAEKCVKCKLCELLCPDFAIAVRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8C6</accession>
    <name>A0A218P8C6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Hydroxyacid dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_06700</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8C6"/>
    <dbReference type="GeneID" id="33315943"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06700"/>
    <dbReference type="OrthoDB" id="34275at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016618">
        <property type="term" value="F:hydroxypyruvate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Glyoxylate reductase (NADP+)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF283">
        <property type="entry name" value="GLYOXYLATE_HYDROXYPYRUVATE REDUCTASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="4">
        <location>
            <begin position="10"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="5">
        <location>
            <begin position="107"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="333" mass="37549" checksum="3E0535C042767BCC" modified="2017-09-27" version="1">MRPKVAVLFKMKSKPTEELKKYADVEFILYPTVEELKERIGEFDGVIISPLNKLPREVIERAGRLKVISCHSAGYDHVDVKAATERGIYVTKVSGVLSEAVAEFAVGLTIALLRKIAYTDKLIRSGKWENQAIIWSSFKDVETVYGKKVGILGMGAIGKAIARRMKAMGTEILYWSRSRKEDVEKDVGAVYKPLEDVLRESDIVILALPATPETYHIINEERIKLLEGKYLVNIGRGTLVDEKAVVKAIEEGKLKGYATDVFEKEPIQEHELFKYEWETVLTPHHAGLSKEAMEDMGFQAVKNLLAVLRGEVPKDLVNREVLKIRPPEEVKML</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8D3</accession>
    <name>A0A218P8D3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_06830</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854305.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8D3"/>
    <dbReference type="GeneID" id="33315969"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06830"/>
    <dbReference type="OrthoDB" id="18209at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03216">
        <property type="entry name" value="ABC_Carb_Monos_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03215">
        <property type="entry name" value="ABC_Carb_Monos_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000127">
        <property type="entry name" value="Ribose import ATP-binding protein RbsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050107">
        <property type="entry name" value="ABC_carbohydrate_import_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43790">
        <property type="entry name" value="CARBOHYDRATE TRANSPORT ATP-BINDING PROTEIN MG119-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43790:SF4">
        <property type="entry name" value="GUANOSINE IMPORT ATP-BINDING PROTEIN NUPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="10"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="264"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ07055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="515" mass="57194" checksum="6E88EF9E2AA6E606" modified="2017-09-27" version="1">MGTTERRPIIEMKGIVKVYPDGTRALKGVDLTVNKGEILGLLGENGAGKTTLMKILFGMLQPTSGKIFINGEEVRFKSPSDAISKGIGMVHQHFTLVEVFDALHNIILGMEGHGLFSKIDVERARKKLQKLMDDLNFQVPLDIPVEDLPVGVQQRIEILKMLYRDVDVLILDEPTAVLTPIEVEELFRVLRQLKAEGKTIIFISHKLNEVLELTDRVTVIRKGEVVGTVNTNEATPQLLARMMVGRDVVLRIEKPPKEAGEPILKVENLKVMGDRGEVAVKNLSFEVRAGEIFGIAGVEGNGQTELIEAISGLRKVSGGRIYLNGKDITGKTPRELYDMGMAHVPEDRTHMGLILDMTVTENSVLGLHWRPKFQRFKGVIHWGKVRKHAQALIEQFDISAPGTEAPVKSLSGGNQQKLIVAREVSKEPVFIIAAQPTRGVDVASTEYIRNYLVKLRNEGKAVLLVSADLDEVLQLSDRMGIIYEGEFMGVVKPEEVTTEEIGMMMGGIRYEEIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8G0</accession>
    <name>A0A218P8G0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphomethylpyrimidine synthase</fullName>
            <ecNumber evidence="10">4.1.99.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Hydroxymethylpyrimidine phosphate synthase</fullName>
            <shortName evidence="10">HMP-P synthase</shortName>
            <shortName evidence="10">HMP-phosphate synthase</shortName>
            <shortName evidence="10">HMPP synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Thiamine biosynthesis protein ThiC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">thiC</name>
        <name evidence="11" type="ORF">A3L08_06900</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + S-adenosyl-L-methionine = 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + CO + 5'-deoxyadenosine + formate + L-methionine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:24840"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58354"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="EC" id="4.1.99.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="10">Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ThiC family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.17" evidence="10"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854319.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8G0"/>
    <dbReference type="GeneID" id="33315983"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06900"/>
    <dbReference type="OrthoDB" id="335406at2157"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070284">
        <property type="term" value="F:phosphomethylpyrimidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.540:FF:000001">
        <property type="entry name" value="Phosphomethylpyrimidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.540">
        <property type="entry name" value="Radical SAM ThiC family, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00089">
        <property type="entry name" value="ThiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037509">
        <property type="entry name" value="ThiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038521">
        <property type="entry name" value="ThiC/Bza_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002817">
        <property type="entry name" value="ThiC/BzaA/B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009895">
        <property type="entry name" value="PRK13352.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00190">
        <property type="entry name" value="thiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30557:SF1">
        <property type="entry name" value="PHOSPHOMETHYLPYRIMIDINE SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30557">
        <property type="entry name" value="THIAMINE BIOSYNTHESIS PROTEIN THIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01964">
        <property type="entry name" value="ThiC_Rad_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00407">
        <property type="entry name" value="phosphomethylpyrimidine_syntha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01114">
        <property type="entry name" value="phosphomethylpyrimidine_syntha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00113">
        <property type="entry name" value="Radical_SAM_Phosphomethylpyrim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 10" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="185"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="226"/>
            <end position="229"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="410"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="414"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ07069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="428" mass="47284" checksum="9A70019B52A02D0B" modified="2017-09-27" version="1">MTQLEAAKRGEITEEMKFVAEREGISPEKLRKSIAKGHTVIFRNVRHDWVKPVAVGNVVRVKVNANIGTSRDIVDVKAEIEKAKVAVKYGADTIMDLSTGGDLDEIRKTIMHAVDVPIGTVPIYQAAEEMLAKGKAIIEMSEDDMWKAVEKHFRDGVDYTTIHVGVTKEVVEKMKRTKRVVGMVSRGGTFLAAWILHWGEENPFYRDYDYLLELAKEYDVVLSLGDGLRPGGLPDAGDELQIAELYTLGRLVRRAREAGVQTMVEGPGHVPIDQIAAQVKLAKVATDNAPFYVLGPIVTDIFPGYDHISAAIGGAIAALNGADFLCYVTPAEHLGLPDVEHVRQGVIAAKIAAHAVNLTRFEADFRKDYLMSLARGRLNWAEQFELSQDKERFIEIRNERPTKTEACSMCGDLCAIKIINDMLAGGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8G6</accession>
    <name>A0A218P8G6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854324.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8G6"/>
    <dbReference type="GeneID" id="33315989"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06930"/>
    <dbReference type="OrthoDB" id="377613at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="75" mass="8610" checksum="1A04E4AF3F5EAFE2" modified="2017-09-27" version="1">MDGFDKYRQLLFFLTWFFVLSLFALVVLREETGTVTFKAALATFFLTLVGIVGTAIKIRRKIREEMAGLPPHEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8J0</accession>
    <name>A0A218P8J0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Chromosome partitioning protein ParB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_07070</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854348.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8J0"/>
    <dbReference type="GeneID" id="33316017"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07070"/>
    <dbReference type="OrthoDB" id="89900at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16400">
        <property type="entry name" value="ParB_Srx_like_nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1760.10">
        <property type="entry name" value="Conserved hypothetical protein from pyrococcus furiosus pfu- 392566-001, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1530.10">
        <property type="entry name" value="Conserved hypothetical protein from pyrococcus furiosus pfu- 392566-001, ParB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023098">
        <property type="entry name" value="SerK/SbnI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040867">
        <property type="entry name" value="SerK_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18231">
        <property type="entry name" value="SerK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="ParB/Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ParB-like N-terminal" evidence="5">
        <location>
            <begin position="19"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="242" mass="27685" checksum="6A67B734A57DA5BA" modified="2017-09-27" version="1">MGVEKVPKYDIPTKKVDYVFIELDKMKPHEQLVQKELEAFIESVTGSGIFWKPMLLAKVPGEDMYLIVDGHHRWAGLQKLGAKRAPSVILDYFSDDVKVYTWYPAFKGDLNEVIERLKAEGLEVIEDLEAEEKAERGEIAFALVGEKSFAIPGGLDEQKKVSKVLDEMSVEGRIELIYYGLKEDAREDMAKGEIDYVFIRKAPTKEEVMELVKRGEVYSPKTTRHVLPFNPDKIDVKLEELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8J6</accession>
    <name>A0A218P8J6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 12">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <shortName evidence="10">GAPDH</shortName>
            <ecNumber evidence="10 12">1.2.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gap</name>
        <name evidence="14" type="ORF">A3L08_07140</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10 12">
            <text>D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = (2R)-3-phospho-glyceroyl phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10300"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.59"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10 12">
            <text>D-glyceraldehyde 3-phosphate + phosphate + NADP(+) = (2R)-3-phospho-glyceroyl phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10296"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.59"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10 12">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 10 12">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10 12">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.59" evidence="10 12"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854362.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8J6"/>
    <dbReference type="GeneID" id="33316031"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07140"/>
    <dbReference type="OrthoDB" id="295712at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00184"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008839">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate reductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047100">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18127">
        <property type="entry name" value="GAPDH_II_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02278">
        <property type="entry name" value="GAPDH_II_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00559">
        <property type="entry name" value="G3P_dehdrog_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000846">
        <property type="entry name" value="DapB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006436">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01546">
        <property type="entry name" value="GAPDH-II_archae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003251">
        <property type="entry name" value="PRK04207.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01113">
        <property type="entry name" value="DapB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10 12" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 10 12" id="KW-0520">NAD</keyword>
    <keyword evidence="4 10 12" id="KW-0521">NADP</keyword>
    <keyword evidence="5 10 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding" evidence="13">
        <location>
            <begin position="3"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10 11">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="12"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="140"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="192"/>
            <end position="193"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ07112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="334" mass="37120" checksum="8123C7BFD99B7700" modified="2017-09-27" version="1">MKVKVGINGYGTIGKRVAYAVSKQDDMELIGVTKTKPDFEAYRAKELGIPVYAASEDFLPRFEKAGFEVAGTLNDLLEKVDVIVDATPGGMGAKNKALYEKAGVKAIFEGGEKASTAEVSFVAQANYEKALGKDYVRVVSCNTTGLTRTLSALQKYIDYVYAVMIRRAADPNDSRRGPVNAITPSVTVPSHHGPDVQTVIPINIETSAFVVPTTLMHVHSVMIELKKPITEKDVIEVFENTTRVLLFEKEKGFDSTAQLIEFARDLHREWNNLYEIAVWKESISVRGNRLFYIQAVHQESDVVPENVDAIRAMFELADKWESIKKTNRSLGILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8M1</accession>
    <name>A0A218P8M1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">Adenylate kinase</fullName>
            <shortName evidence="4">AK</shortName>
            <ecNumber evidence="4 6">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">ATP:AMP phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Adenylate monophosphate kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">adk</name>
        <name evidence="8" type="ORF">A3L08_07270</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 6">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from ADP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 6">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="4">Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis. Some bacteria have evolved a zinc-coordinating structure that stabilizes the LID domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the adenylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="4 6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854386.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8M1"/>
    <dbReference type="GeneID" id="33316057"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07270"/>
    <dbReference type="OrthoDB" id="31230at2157"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00649"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000106">
        <property type="entry name" value="Adenylate kinase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006259">
        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007862">
        <property type="entry name" value="Adenylate_kinase_lid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01351">
        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001387">
        <property type="entry name" value="PRK00279.2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05191">
        <property type="entry name" value="ADK_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="2 4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Adenylate kinase active site lid" evidence="7">
        <location>
            <begin position="125"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="NMP" evidence="4">
        <location>
            <begin position="30"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="4">
        <location>
            <begin position="124"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="57"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="83"/>
            <end position="86"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="134"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="223" mass="25552" checksum="88CA420B9F64C536" modified="2017-09-27" version="1">MNILIFGPPGSGKSTHSRTIVERYGLIYISSGDMIRAEISAGTELGREMKSYLERGDLIPDTVVNTLIISKLRRHRKNFIIDGYPRTAEQVLALENYLYDHGLSIDVALEIFISKDESVERISGRRICPKCGAVYHLKYRPSRVPRKCDVCGTDLIQRDDDKPEIVARRYDLYIKNMEPIIKFYKKQGVYVRVDGHDSIEGVWERIRPLLDYIKAREDSSQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8M5</accession>
    <name>A0A218P8M5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_07275</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8M5"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07275"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="248" mass="27691" checksum="EEDED574310F96DB" modified="2017-09-27" version="1">MLNVDPPNYYPIINKLFAARLAEALEALGIQLDSIEVRVVPEGRTLRLIFKLVISGMEGFVPIDVQETVQGILEKLADDATTTFGRLYSVRFEVEDFELVRAPQRNGWEANVKLIVNGPDEKLERLNRLGRGLVITLKEWGIAISALTVSMPLNGPGSLRIVIKAEAELSEAEKNELAERVRTKACSFMKTLFGSACPVQVRIIDPKDRTMKIVLREEERLEALVKELSNDEDIKRLLSLLGKSPPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8R1</accession>
    <name>A0A218P8R1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha-galactosidase NEW3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_07560</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854436.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8R1"/>
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    <dbReference type="OrthoDB" id="110363at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1120">
        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018905">
        <property type="entry name" value="A-galactase_NEW3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39198:SF1">
        <property type="entry name" value="ALPHA-GALACTOSIDASE NEW3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39198">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10633">
        <property type="entry name" value="NPCBM_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="686"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-galactosidase NEW3" evidence="2">
        <location>
            <begin position="589"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="711" mass="75652" checksum="52B149F8161CF5F1" modified="2017-09-27" version="1">MKKVLAFLLSVALLLPLVGAQPYVTVFEGRLAGGHAVVVGDYTITIVKSAGGSPYSMYLMLRNGSRILELKPFVFGTEIERDDIRIIMGSYTPDGGFIVVSVKPRFVGSLEPKVGAKLSFNGTTVEVTAVGNRTIDVSVNGVVRTLEVNGSAVVDLVALEYNGKEVKVYAASPASETISADYTVFYPYGKVKASGPVDLPITVASSTDAELTLPLRVLSLPEGWSASFLYGGIEVGEISVPPRGTVSLILHIEPISSGAIRFAVGNFTGTLEVESAGIEVSLPYLSLEAEAGTALSVPVSFSGTGKVEFEPGELPEGWTVYLTNGQYRLRSFEVEGSFTASLMVEVPRNATLGDHRIGFTLNGRAYSLDVYVYKTYLGQPAKLTVVLTDESGNPVRGWVAVEDRNVTTSPAGSVDLELKPGEYTVTAGAEGCSPVSEKVKLSDGEEKTLQLELKRAEYYFTAKLERDVLTTGPSGSGSTEIVIQNLGSRDDEYRVTVEGLPDGWIYVVSQDPNGAVPVGSIKVDSGDSATAYLVLFPPFNVESGDLNARVVVSGSASKRELPLTIHVENPAFLRLDAEMPSLTVRAGGSTATNLWVDATGTVTNVKFTVQAPNGWDVEVLPPTISRVGAEAYGNVVTYPGPNRVELRVRVPKSAPAGTYTITVTAAGDQAKAETVITVRVTQGSSGTWIGVVLLVLAFGVVIWLMRRVGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8S5</accession>
    <name>A0A218P8S5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Molybdopterin molybdenumtransferase MoeA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_07650</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MoeA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854451.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8S5"/>
    <dbReference type="GeneID" id="33316133"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07650"/>
    <dbReference type="OrthoDB" id="31371at2157"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.190.11:FF:000001">
        <property type="entry name" value="Molybdopterin molybdenumtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.340.10:FF:000005">
        <property type="entry name" value="Molybdopterin molybdenumtransferase MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="entry name" value="MoeA, C-terminal, domain IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.10">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.190.11">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045515">
        <property type="entry name" value="Glp_gephyrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="MOLYBDOPTERIN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192:SF19">
        <property type="entry name" value="MOLYBDOPTERIN BIOSYNTHESIS PROTEIN MJ0666-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="MoeA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="MoeA N-terminal region -like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="4">
        <location>
            <begin position="186"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="396" mass="43731" checksum="877F593A37046D9B" modified="2017-09-27" version="1">MREFKKLTPYKEALRLMLESLAEIGEVEEVPLDGALGRVLAEDIVSPIDSPPFDRAAVDGYALRAEDTFQAREYNPVEIKVIDEIVAGEKSGAKVEPGTAVKLTTGAKIPEGANAVLMQEMAERERDIIRVLRPVAPGQNVAFAGEDVKKGEVVLRKGQILRPQDLALLKSIGLKTVRVKRKPLVGIIVTGDELIEEFDEGALKAGKILDSNSVMLKGLVRQYFGEPRFYGVIPDDEERIGEAIRKAREECDLVLITGGSAFGDKDFAHRFVSLLFHGTTIKPGRPIGYGEKTFVMSGYPAAVFTQFHLYVKHALAKLVGARDYEVKVRARLTERVPSQLGRYEFIKVRYENGEARPIRKKGSGIISSLVESNGYIGIPEDSEGYLEGELVEVVLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8V6</accession>
    <name>A0A218P8V6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">tRNA (guanine(37)-N(1))-methyltransferase Trm5b</fullName>
            <ecNumber evidence="2">2.1.1.228</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">M1G-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">tRNA [GM37] methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">A3L08_07715</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Specifically methylates the N1 position of guanosine-37 in various tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>guanosine(37) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36899"/>
            <dbReference type="Rhea" id="RHEA-COMP:10145"/>
            <dbReference type="Rhea" id="RHEA-COMP:10147"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.228"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.228" evidence="2"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854460.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8V6"/>
    <dbReference type="GeneID" id="33316145"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07715"/>
    <dbReference type="OrthoDB" id="8079at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052906">
        <property type="term" value="F:tRNA (guanine(37)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.110:FF:000001">
        <property type="entry name" value="tRNA (guanine(37)-N1)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000131">
        <property type="entry name" value="tRNA wybutosine-synthesizing protein 2/3/4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.110">
        <property type="entry name" value="Met-10+ protein-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056744">
        <property type="entry name" value="TRM5/TYW2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040601">
        <property type="entry name" value="Trm5a/b_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047730">
        <property type="entry name" value="tRNAMtaseTrm5b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF36">
        <property type="entry name" value="TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18093">
        <property type="entry name" value="Trm5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25133">
        <property type="entry name" value="TYW2_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 14" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="13">
        <location>
            <begin position="83"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ07212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="330" mass="37856" checksum="2A980CA7D6B6215D" modified="2017-09-27" version="1">MLAVKVPKREAEKIRKKLLELGVLAKGYAIKREGDFVLFPVTGQVEGFELVESEFERLEKRYHSYREVVEVPEELRPLLPSSFDIIGDIAIIELPEKLMPYGRVIGEAILKVHRHIKAVFAKGSKVEGEYRVRELIHLAGESRTETVHRENGIRLKLDVARVYYTPRLATERRRVFEKTQPGEVVFDMFAGVGPYSILLAKRAKLVFACDINPWAIRYLEENVRLNKVNNVVPILGDVRKVAGKIKADRVVMNLPKFADRFLREAMMSVKPGGIVHYYGFGPEEDLFSEHEVKIKAVAKELGLSVEFLDRRKVRPYAPRQFNIAIDFKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8X8</accession>
    <name>A0A218P8X8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glyceraldehyde 3-phosphate phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_07900</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the dephosphorylation of D,L-glyceraldehyde 3-phosphate in vitro.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854495.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8X8"/>
    <dbReference type="GeneID" id="33316185"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07900"/>
    <dbReference type="OrthoDB" id="27736at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051400">
        <property type="entry name" value="HAD-like_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011950">
        <property type="entry name" value="HAD-SF_hydro_IA_CTE7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006549">
        <property type="entry name" value="HAD-SF_hydro_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02253">
        <property type="entry name" value="CTE7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01662">
        <property type="entry name" value="HAD-SF-IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46470:SF2">
        <property type="entry name" value="GLYCERALDEHYDE 3-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46470">
        <property type="entry name" value="N-ACYLNEURAMINATE-9-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01135">
        <property type="entry name" value="C1.5.6:_HAD__Beta-PGM__Phospha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="244" mass="28188" checksum="77A9E2BA2AA04289" modified="2017-09-27" version="1">MIKVVFFDLDDTIVDTTKLAEMARRNAIENMVRHGLPVDFDTAYQELLELISEYGSNFSRHFDYLLRRLDLPNNPKWIAAGVISYHNTKFAYLRTVKGVRQVLLDLQRSGYRLGIITDGDPIKQWEKILRLELDEYFGGVFISDHLGVKKPHPKIFRKALRKMGVEPGEAIMVGDRLYSDIYGAKQVGMRTVWFKYGKYADRELEYLDYADFTISSLEELPEILRGLKLEEESEGGSDKEVHAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Y9</accession>
    <name>A0A218P8Y9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Coenzyme A biosynthesis bifunctional protein CoaBC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">DNA/pantothenate metabolism flavoprotein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Phosphopantothenoylcysteine synthetase/decarboxylase</fullName>
            <shortName evidence="3">PPCS-PPCDC</shortName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenoylcysteine decarboxylase</fullName>
                <shortName evidence="3">PPC decarboxylase</shortName>
                <shortName evidence="3">PPC-DC</shortName>
                <ecNumber evidence="3">4.1.1.36</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaC</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenate--cysteine ligase</fullName>
                <ecNumber evidence="3">6.3.2.5</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaB</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="3">Phosphopantothenoylcysteine synthetase</fullName>
                <shortName evidence="3">PPC synthetase</shortName>
                <shortName evidence="3">PPC-S</shortName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="3" type="primary">coaBC</name>
        <name evidence="6" type="ORF">A3L08_07840</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes two sequential steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. In the second step the latter compound is decarboxylated to form 4'-phosphopantotheine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(R)-4'-phosphopantothenate + L-cysteine + CTP = N-[(R)-4-phosphopantothenoyl]-L-cysteine + CMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19397"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="6.3.2.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>N-[(R)-4-phosphopantothenoyl]-L-cysteine + H(+) = (R)-4'-phosphopantetheine + CO2</text>
            <dbReference type="Rhea" id="RHEA:16793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="4.1.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="3">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the PPC synthetase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.36" evidence="3"/>
    <dbReference type="EC" id="6.3.2.5" evidence="3"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854484.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Y9"/>
    <dbReference type="GeneID" id="33316172"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07840"/>
    <dbReference type="OrthoDB" id="10536at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071513">
        <property type="term" value="C:phosphopantothenoylcysteine decarboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004632">
        <property type="term" value="F:phosphopantothenate--cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015941">
        <property type="term" value="P:pantothenate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10300">
        <property type="entry name" value="CoaB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02225">
        <property type="entry name" value="CoaBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035929">
        <property type="entry name" value="CoaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005252">
        <property type="entry name" value="CoaBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007085">
        <property type="entry name" value="DNA/pantothenate-metab_flavo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00521">
        <property type="entry name" value="coaBC_dfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359">
        <property type="entry name" value="HOMO-OLIGOMERIC FLAVIN CONTAINING CYS DECARBOXYLASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359:SF6">
        <property type="entry name" value="PHOSPHOPANTOTHENOYLCYSTEINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04127">
        <property type="entry name" value="DFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102645">
        <property type="entry name" value="CoaB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="4">
        <location>
            <begin position="19"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="DNA/pantothenate metabolism flavoprotein C-terminal" evidence="5">
        <location>
            <begin position="193"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenoylcysteine decarboxylase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenate--cysteine ligase" evidence="3">
        <location>
            <begin position="198"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="404" mass="44191" checksum="285B8725F986B3F3" modified="2017-09-27" version="1">MLHHVRLIYATKSRKLVGKKIVLAIPGSIAAVECVKLARELIRHGAEVHAVMSENAQKIIHPYAMEFATGNPVVTEITGFIEHVELAGEHENKADLVLVCPATANTIGKIANGIDDTPVTTVVTTAFAHTPIMIAPAMHSTMYDHPIVVENIEKLKKLGVEFIEPRFEEGKAKVASIDEIVYRVIKKLHPKTLAGKRVLVTAGATREYIDPIRYITNASSGKMGVALAEEAELRGAEVTLIKTRGSVSSFVENQIEVETVEEMLAAIENELKAKKYDVVVLAAAVSDFRVKEKADVKIKSGKPLTLELEPTPKIIDRVKELQPDTFLVGFKAETGLSEEELIAAARKQIERAGSDLVVANTLSAFGSEENEVLLVTGDSVKRLPKMGKRELAERLWDEIISILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P975</accession>
    <name>A0A218P975_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rubrerythrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08345</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854576.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P975"/>
    <dbReference type="GeneID" id="33316275"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08345"/>
    <dbReference type="OrthoDB" id="100171at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01045">
        <property type="entry name" value="Ferritin_like_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rr_diiron-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33531:SF10">
        <property type="entry name" value="BLR7895 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33531">
        <property type="entry name" value="RUBRERYTHRIN SUBFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rubrerythrin diiron-binding" evidence="1">
        <location>
            <begin position="5"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="159" mass="18641" checksum="611FE6BCD40C28B0" modified="2017-09-27" version="1">MNELEALALALEVEKAELKFYIKLAKKAKDERAKKMFLFLAGEEAEHWDIFEEKFVEALVEKCELPVVDKELLEKLVVQVYEENLSEVDAVKIGMEQEKLTWEFYEKAAKEAQHESVRRTFEELAKVEKAHYELLKAQYDAVMKTGIWMDYQDSSLEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9C5</accession>
    <name>A0A218P9C5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 13 14">Adenylosuccinate lyase</fullName>
            <shortName evidence="14">ASL</shortName>
            <ecNumber evidence="5 13 14">4.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 14">Adenylosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">A3L08_08565</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes two reactions in de novo purine nucleotide biosynthesis. Catalyzes the breakdown of 5-aminoimidazole- (N-succinylocarboxamide) ribotide (SAICAR or 2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate) to 5-aminoimidazole-4-carboxamide ribotide (AICAR or 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) and fumarate, and of adenylosuccinate (ADS or N(6)-(1,2-dicarboxyethyl)-AMP) to adenosine monophosphate (AMP) and fumarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + fumarate</text>
            <dbReference type="Rhea" id="RHEA:23920"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:23921"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-(1,2-dicarboxyethyl)-AMP = fumarate + AMP</text>
            <dbReference type="Rhea" id="RHEA:16853"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:16854"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 14">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer. Residues from neighboring subunits contribute catalytic and substrate-binding residues to each active site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.2" evidence="5 13 14"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854615.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9C5"/>
    <dbReference type="GeneID" id="33316318"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08565"/>
    <dbReference type="OrthoDB" id="7033at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00132"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00336"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070626">
        <property type="term" value="F:(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004018">
        <property type="term" value="F:N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01360">
        <property type="entry name" value="Adenylsuccinate_lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.10:FF:000012">
        <property type="entry name" value="Adenylosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.40.30:FF:000007">
        <property type="entry name" value="Adenylosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000008">
        <property type="entry name" value="Adenylosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019468">
        <property type="entry name" value="AdenyloSucc_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004769">
        <property type="entry name" value="Pur_lyase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00928">
        <property type="entry name" value="purB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43172">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43172:SF1">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10397">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00145">
        <property type="entry name" value="ARGSUCLYASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00998">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14 16" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 14" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Adenylosuccinate lyase C-terminal" evidence="15">
        <location>
            <begin position="360"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="NCBIfam" id="TIGR00928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ASJ07368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="451" mass="51239" checksum="2A17E26E83DC1613" modified="2017-09-27" version="1">MAVHPIDYRYGSEEMRRIWDEENKLQKLLDVEAALARAHAKLGNIPEESARVISERANTEWVKLERVKEIEAEIHHDIMAVVKALSEVCGEHGKYVHLGATSNDIIDTANALLIKESLEIVERDLKELRSVLKKLAKEHKYTVCIGRTHGQHAVPTTYGMKFAIWLDEVQRHIDRLNELKERVLVGQMSGAVGTMASFGEKGLEIQRLVMEDLGLKPARISNQIIQRDVYAELMAFLALVASTLDKIALEIRNLQRTEILEVSEPFGEKQVGSSTMPHKRNPIRSEKVSGLARVLYSNVIPALLNNPLWHERDLTNSSVERVILPESFVLIDEMLKNTIKVLSGLEFFPENIERNLYMTNNLIMAEPLMLKLAEKGMGRQEAHELVRRLAMKAFREGRDLLEVVKESEEVRKFLSDDDIASLKPENYIGLAPQIVDSVIAFIEGKEREEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9J2</accession>
    <name>A0A218P9J2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Methylmalonyl-CoA mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_09035</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854696.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9J2"/>
    <dbReference type="GeneID" id="33316413"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09035"/>
    <dbReference type="OrthoDB" id="9041at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02071">
        <property type="entry name" value="MM_CoA_mut_B12_BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.280">
        <property type="entry name" value="Cobalamin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006159">
        <property type="entry name" value="Acid_CoA_mut_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00640">
        <property type="entry name" value="acid_CoA_mut_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48101:SF3">
        <property type="entry name" value="COENZYME B12-DEPENDENT MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48101">
        <property type="entry name" value="METHYLMALONYL-COA MUTASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="Cobalamin (vitamin B12)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="5" id="KW-0170">Cobalt</keyword>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B12-binding" evidence="6">
        <location>
            <begin position="6"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="143" mass="15458" checksum="553DD6908E01FA83" modified="2017-09-27" version="1">MVERSKVRVLVAKPGLDGHDRGAKVVARALRDAGFEVIYTGIRQTPEQIVESVIQEDVDVLGISILSGAHMVLIPKILKLLEEKGLKVGEDVLVIAGGIIPPDDAEQLEKMGVAKVFGPGSPIEAIIRFIDENVPKLKKFRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9L0</accession>
    <name>A0A218P9L0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleotide-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854716.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9L0"/>
    <dbReference type="GeneID" id="33316435"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09145"/>
    <dbReference type="OrthoDB" id="91344at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09854">
        <property type="entry name" value="PIN_VapC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002850">
        <property type="entry name" value="PIN_toxin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34610:SF4">
        <property type="entry name" value="SLL8027 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34610">
        <property type="entry name" value="SSL7007 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="2"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="151" mass="17089" checksum="AA5E08D5AE90BA02" modified="2017-09-27" version="1">MIRVYLDTNVLLNVWFKEENPKTGEKLWEASLQILKLIENGKIEGVVSIFTLMESAHVFKRNKLNPERVKEIEDVGIEVYIPNELTLIDAFSFQLKLGTDPYDSVALASALASGCDVLITRDEDFRKKAENQIMILSPEEFLEWIAASGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9P1</accession>
    <name>A0A218P9P1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Potassium channel protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854748.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9P1"/>
    <dbReference type="GeneID" id="33316472"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09330"/>
    <dbReference type="OrthoDB" id="85913at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="entry name" value="Phosphate transport system protein phou homolog 2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="entry name" value="Regulator of K+ conductance, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050144">
        <property type="entry name" value="AAE_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30445">
        <property type="entry name" value="K(+)_H(+) ANTIPORTER SUBUNIT KHTT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30445:SF8">
        <property type="entry name" value="K(+)_H(+) ANTIPORTER SUBUNIT KHTT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109755">
        <property type="entry name" value="PhoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="TrkA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0407">Ion channel</keyword>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RCK C-terminal" evidence="1">
        <location>
            <begin position="102"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="190" mass="21404" checksum="4489C08194881DDA" modified="2017-09-27" version="1">MSEFDEIRNCLVEMKDLSALMIDLAFSSVMYNSEDIAEEVYLLEEKMDDLTLKVKKLALRAAKHEEDPESLLSIIDLADINERISDAAYGIADIILRDIEPHPIIRKIMEDTEEELGRVTVRAGSILIGKSLKQLKLPSKIGTRILAIKRGTRYIYNPGKDDVILEGDTLIAVSSDLDKLRKLAGEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9S2</accession>
    <name>A0A218P9S2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein eL39</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">rpl39e</name>
        <name evidence="6" type="ORF">A3L08_09505</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL39 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012572069.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9S2"/>
    <dbReference type="SMR" id="A0A218P9S2"/>
    <dbReference type="GeneID" id="28494736"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09505"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1620.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein-like L39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1620.10">
        <property type="entry name" value="Ribosomal protein L39e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00629">
        <property type="entry name" value="Ribosomal_eL39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000077">
        <property type="entry name" value="Ribosomal_eL39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023626">
        <property type="entry name" value="Ribosomal_eL39_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002316">
        <property type="entry name" value="PRK01242.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00832">
        <property type="entry name" value="Ribosomal_L39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48662">
        <property type="entry name" value="Ribosomal protein L39e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="51" mass="6220" checksum="29510F3A159553F2" modified="2017-09-27" version="1">MARNKPLAKKLRLAKAAKQNRRVPVWVIVKTNRKVMTHPKRRMWRRTKLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9T3</accession>
    <name>A0A218P9T3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LSM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09555</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854790.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9T3"/>
    <dbReference type="GeneID" id="33316517"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09555"/>
    <dbReference type="OrthoDB" id="84581at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00600">
        <property type="entry name" value="Sm_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="71" mass="8119" checksum="B538EC30B5F0B1F5" modified="2017-09-27" version="1">MSEKQYLLDKTLESWKGKRVALSVSNEHSFTGILEDFDEEVILLRDVVDIAGNKAKALVVKIEDLNWIMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9V2</accession>
    <name>A0A218P9V2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09670</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854811.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9V2"/>
    <dbReference type="GeneID" id="33316540"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09670"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045397">
        <property type="entry name" value="TumE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20126">
        <property type="entry name" value="TumE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="129" mass="15085" checksum="7AF4FE657F91A584" modified="2017-09-27" version="1">MPRELELLEKSPAVKSYEIVDYKEGESFYFLKIRAELIDGSVLYIREFVSEEEYNYSFQWQKDEKLIVRWDNAPHHRDIETFPHHKHVGSQDSIHPSKEISLEDILGVIGEKIVPGNRAQDSNASIRVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9V6</accession>
    <name>A0A218P9V6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09420</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854764.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9V6"/>
    <dbReference type="GeneID" id="33316490"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09420"/>
    <dbReference type="OrthoDB" id="17710at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="255" mass="28939" checksum="DE3E7E36BB8938BE" modified="2017-09-27" version="1">MRVAIVTSDPRIYYVSTKVLKEYGIPFRSLRVGEKIPFDVEVVLTSESDYDRIDFPVKVVVRDEGFIDELLSKLEGRERFQRIYIAIDPGERPGLSVVADNRVIEVHHLKNPRDVGIIIDLLEKYPGAKIKIGHGAKRQRVLMLKALADLLGYDYPVIIVNESRTTPKVGGMEVSQVQDIVAAINIGLREGREVSIGEILEVKEPTKREIEDIKRRSRELSGNITISSKLAREVAVGNLTLEEAIERQRKRRRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9W9</accession>
    <name>A0A218P9W9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphoserine phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_09740</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.3" evidence="3"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854824.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9W9"/>
    <dbReference type="GeneID" id="33316554"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09740"/>
    <dbReference type="OrthoDB" id="10041at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036424">
        <property type="term" value="F:L-phosphoserine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050582">
        <property type="entry name" value="HAD-like_SerB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006386">
        <property type="entry name" value="HAD-SF_hydro_IB_PSP-like_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01488">
        <property type="entry name" value="HAD-SF-IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01491">
        <property type="entry name" value="HAD-SF-IB-PSPlk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344:SF2">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0718">Serine biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="206" mass="23258" checksum="A56DF4379D6E0A2E" modified="2017-09-27" version="1">MVRLIAFDLEGTLVKSISSWVELHKKFGTWEKGKEYAELFFSGEIDYVKWAELDSSLWKGHTRDEILEWANSVEYMDGAEELIEFLRKNDFKIAILSSGLMCLAGRIARELGVDYVYANELIFDENGVVTGKVNPLVDFKSKGVILRELKEELKPKLTVAVGDGYNDISMFREADVAIAINPHEGVEGDHNVESLREVKEIIAGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Y2</accession>
    <name>A0A218P9Y2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ECF transporter S component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Y2"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01090"/>
    <dbReference type="OrthoDB" id="85595at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1760.20">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="194" mass="21145" checksum="038985E7EE818921" modified="2017-09-27" version="1">MSSREIAVIGIMLGLSLMFEVIPIEMPTIWGMKIDFVAVPIIMAYLLTGFIGGLTAVLLLFAGLSIVSSASWLGGMMKATATFTVLVGLETARRMTKFSLENPTRGQLIRFAVLAYLIGVALRIPLMVALNYYVALEIWLGIPQEKVVETVEAWTGVPFWVAIGLPNAIQSAIDVFIGLLATLPVLRRVPHLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA08</accession>
    <name>A0A218PA08_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Uridylate kinase</fullName>
            <shortName evidence="11">UK</shortName>
            <ecNumber evidence="11">2.7.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Uridine monophosphate kinase</fullName>
            <shortName evidence="11">UMP kinase</shortName>
            <shortName evidence="11">UMPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">pyrH</name>
        <name evidence="13" type="ORF">A3L08_00665</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the reversible phosphorylation of UMP to UDP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>UMP + ATP = UDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:24400"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.22"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="11">Inhibited by UTP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; UDP from UMP (UMPK route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the UMP kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.22" evidence="11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854848.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA08"/>
    <dbReference type="GeneID" id="33314737"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00665"/>
    <dbReference type="OrthoDB" id="372251at2157"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00275"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033862">
        <property type="term" value="F:UMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006225">
        <property type="term" value="P:UDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04253">
        <property type="entry name" value="AAK_UMPK-PyrH-Pf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000030">
        <property type="entry name" value="Uridylate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01220_A">
        <property type="entry name" value="PyrH_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011817">
        <property type="entry name" value="Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011818">
        <property type="entry name" value="Uridylate_kinase_arch/spir"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02076">
        <property type="entry name" value="pyrH_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42833">
        <property type="entry name" value="URIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42833:SF4">
        <property type="entry name" value="URIDYLATE KINASE PUMPKIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005650">
        <property type="entry name" value="Uridylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/glutamate/uridylate kinase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="9"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="114"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ07603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="225" mass="24671" checksum="FD7CD7E77712D619" modified="2017-09-27" version="1">MRIVFDIGGSVLVPDDPDIDFIKAIAYELVKISEDHEVAVVVGGGKVARKYIKAAKEFTPNETFKDYIGIHITRANAMLLIAALGEKAYPFVIQDFRKAWEVIQLKKIPIMGGTHPGHTTDAVATLLAEYLQADLLVVVTNVDGVYDSDPRKNPNAKKLDRITPEELVDIAMEAESRAGGSGVVDALAAKFIQRGRIRTYIVGKEDAYHLFDVVKGRHSGTVVEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA13</accession>
    <name>A0A218PA13_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">KaiC-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA13"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01165"/>
    <dbReference type="OrthoDB" id="85875at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11570">
        <property type="entry name" value="Protein of unknown function DUF257"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005489">
        <property type="entry name" value="DUF257"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03192">
        <property type="entry name" value="DUF257"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="214" mass="23724" checksum="1A211A6CF056A8FA" modified="2017-09-27" version="1">MALQAVDSILFGLRSGETVLVEYGSTSSPELLLYLICRRCGDRRRPVLLDDISDSFAEALTRLELVGLNLEGLSNVPVIKIGGNRDVGSVFGRVEVDKHSLDTKYYDRLYFSAAEQELVLNPVLGFHKFFLVIPDRELLRLIRNISTFVGRSGRAAVYLVNRDVVERSQLSGHYHLFREIATTIVSIEQSGDGYVGGVPKAADDGLRGVRIPMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA30</accession>
    <name>A0A218PA30_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA-directed RNA polymerase subunit Rpo3</fullName>
            <ecNumber evidence="5">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">DNA-directed RNA polymerase subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpo3</name>
        <name evidence="5" type="synonym">rpoD</name>
        <name evidence="7" type="ORF">A3L08_04230</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="5"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA30"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04230"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07030">
        <property type="entry name" value="RNAP_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="entry name" value="DNA-directed RNA polymerase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00320">
        <property type="entry name" value="RNApol_arch_Rpo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001514">
        <property type="entry name" value="DNA-dir_RNA_pol_30-40kDasu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022842">
        <property type="entry name" value="RNAP_Rpo3/Rpb3/RPAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050518">
        <property type="entry name" value="Rpo3/RPB3_RNA_Pol_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001988">
        <property type="entry name" value="PRK00783.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800:SF2">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="Insert subdomain of RNA polymerase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00446">
        <property type="entry name" value="RNA_POL_D_30KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 5 7" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RpoA/D/Rpb3-type" evidence="6">
        <location>
            <begin position="14"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="260" mass="29592" checksum="479A5FB054DE0D78" modified="2017-09-27" version="1">MKPEFEVLEKREDSMKFILRGVDVAFANALRRTILAEVPTFAVDEVEFFENDSALFDEIIAHRLAMIPLTTPHERFSLDSLELDDYTVTLSLEAEGPGMVYSGDLKSDDEGVKPANPDIPIVKLAEGQKLTLNAYAKLGRGKDHAKWQPGFVYYKYLTRIHVDKEVPDWKELKALAERRGLPVEETDGELVITTTKAFYLPRKFDEYVGDKIREEIVPGSFVFTVETNGELPVEEIVTIALKILMRKSDRFINELHKLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA54</accession>
    <name>A0A218PA54_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">rRNA small subunit methyltransferase F RNA-binding PUA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07545</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854917.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA54"/>
    <dbReference type="GeneID" id="33316112"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07545"/>
    <dbReference type="OrthoDB" id="50259at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027391">
        <property type="entry name" value="Nol1_Nop2_Fmu_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13636">
        <property type="entry name" value="Methyltranf_PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="rRNA small subunit methyltransferase F RNA-binding PUA-like" evidence="1">
        <location>
            <begin position="100"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="152" mass="17469" checksum="D79CDAEC7EAAE1F9" modified="2017-09-27" version="1">METPRNPREEVGKADDTELVKKLLIENYGYAPDLVYEIRGRYHKVYAWKPCSIEIKGPDRQGIYFARVESDGIRLSIEGSFLVGPKATKNVVELDDERVRRYLAGESVEIGDRELHGWVIIKWRSYFLGSAKAKGGKLINYVPGERRLRLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA78</accession>
    <name>A0A218PA78_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Probable thymidylate kinase</fullName>
            <ecNumber evidence="2 11">2.7.4.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 11">dTMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">tmk</name>
        <name evidence="13" type="ORF">A3L08_04900</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>dTMP + ATP = dTDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:13517"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58369"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the thymidylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="2 11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854893.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA78"/>
    <dbReference type="GeneID" id="33315584"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04900"/>
    <dbReference type="OrthoDB" id="43083at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:dTMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006227">
        <property type="term" value="P:dUDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01672">
        <property type="entry name" value="TMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000225">
        <property type="entry name" value="Thymidylate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018095">
        <property type="entry name" value="Thymidylate_kin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00041">
        <property type="entry name" value="DTMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344:SF4">
        <property type="entry name" value="UMP-CMP KINASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01331">
        <property type="entry name" value="THYMIDYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate kinase-like" evidence="12">
        <location>
            <begin position="5"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="7"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ07650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="205" mass="23261" checksum="11339D9104F940B7" modified="2017-09-27" version="1">MFVVIEGIDGAGKSTQAKLLAKWFEERGYEVVLTKEPTDTAFGKLIRKLVLTGGKEGIIDGARISHEAEALLFAADRAEHVAKLIKPSIEAGKIVISDRYFYSSLAYQWARGLDLEWLIDLNRFAVRPDLVILLDLPVKESMKRINGRSIKTEFDKIAELQKKVRENYLKLTERFPEMRIVNALASVEDIHNDIVALVEHELLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P558</accession>
    <name>A0A218P558_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydroxymethylglutaryl-CoA synthase</fullName>
            <shortName evidence="4">HMG-CoA synthase</shortName>
            <shortName evidence="4">HMGCS</shortName>
            <ecNumber evidence="4">2.3.3.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_00530</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the condensation of acetyl-CoA with acetoacetyl-CoA to form 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA). Functions in the mevalonate (MVA) pathway leading to isopentenyl diphosphate (IPP), a key precursor for the biosynthesis of isoprenoid compounds that are building blocks of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>acetoacetyl-CoA + acetyl-CoA + H2O = (3S)-3-hydroxy-3-methylglutaryl-CoA + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10188"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57286"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with acetoacetyl-CoA thiolase that catalyzes the precedent step in the pathway and with a DUF35 protein. The acetoacetyl-CoA thiolase/HMG-CoA synthase complex channels the intermediate via a fused CoA-binding site, which allows for efficient coupling of the endergonic thiolase reaction with the exergonic HMGCS reaction.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the thiolase-like superfamily. Archaeal HMG-CoA synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.10" evidence="4"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853189.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P558"/>
    <dbReference type="GeneID" id="33314710"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00530"/>
    <dbReference type="OrthoDB" id="5812at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004421">
        <property type="term" value="F:hydroxymethylglutaryl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00827">
        <property type="entry name" value="init_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000046">
        <property type="entry name" value="UPF0219 protein M1627_1703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01409">
        <property type="entry name" value="HMG_CoA_synth_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004656">
        <property type="entry name" value="HMG_CoA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00748">
        <property type="entry name" value="HMG_CoA_syn_Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003274">
        <property type="entry name" value="PRK04262.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323:SF2">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2 4" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Beta-ketoacyl-[acyl-carrier-protein] synthase III C-terminal" evidence="5">
        <location>
            <begin position="224"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="4">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="4">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="4">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>(3S)-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>(3S)-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>(3S)-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <note>ligand shared with acetoacetyl-CoA thiolase</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>(3S)-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>(3S)-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <note>ligand shared with acetoacetyl-CoA thiolase</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>(3S)-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>(3S)-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ05926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="350" mass="38014" checksum="384BBCC17F16FF4E" modified="2017-09-27" version="1">MGKLLKPNREVGIIGYGAYVPMYRIKAEEIGRVWGVSSFPIQEKSVPGLDEDTITIGIEAARNALKRAKIDPKLIRAVWLGTESKPYAVKPSGTVIAAAIGATPDLNAADFEFACKAGTEAIQAAIGFVGSEMADYAMAIGADTSQGRPGDHLEFTAAAGGAAYILAPKSSETVAYFEASYSYVTDTPDFWRRQHEHYPRHGNRFTGEPAYFHQIINAAKTLMEELDYSPSDFDYAVFHQPNVKFPLTAAKILGIPKEKVLPGLLSGIIGNTYSGATLVGVSAVLDIAKPGDRILWVSFGSGAGSDAFSLVVQDAIEEKRDLAPKTMDYVNRKKYIDYALYAKHRGKYIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P568</accession>
    <name>A0A218P568_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Osmotically inducible protein OsmC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_00570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853196.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P568"/>
    <dbReference type="GeneID" id="33314718"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00570"/>
    <dbReference type="OrthoDB" id="84927at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003718">
        <property type="entry name" value="OsmC/Ohr_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036102">
        <property type="entry name" value="OsmC/Ohrsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34352">
        <property type="entry name" value="PROTEIN YHFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34352:SF1">
        <property type="entry name" value="PROTEIN YHFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02566">
        <property type="entry name" value="OsmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82784">
        <property type="entry name" value="OsmC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ05933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="136" mass="15365" checksum="26A1F5CDF8D87150" modified="2017-09-27" version="1">MSEPVKGKVEWFKDYQFVGHIEGDKCSVILGEGGISPMKLLLLSVAGCTAYDVVMILQKMREPIKGLEVEISGERREEHPRIYSKVHLHYKIHGNVSEEKAKRAIELSQDKYCSASAHIKLSGAKLTYSFEIVRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P577</accession>
    <name>A0A218P577_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">C/D box methylation guide ribonucleoprotein complex aNOP56 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_00545</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NOP5/NOP56 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853192.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P577"/>
    <dbReference type="GeneID" id="33314713"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00545"/>
    <dbReference type="OrthoDB" id="11877at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.90:FF:000007">
        <property type="entry name" value="Pre mRNA splicing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045056">
        <property type="entry name" value="Nop56/Nop58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048896">
        <property type="entry name" value="Nop5_56-rel_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047099">
        <property type="entry name" value="Nop5_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011121">
        <property type="entry name" value="PRK14552.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894">
        <property type="entry name" value="NUCLEOLAR PROTEIN 5 NUCLEOLAR PROTEIN NOP5 NOP58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21572">
        <property type="entry name" value="Nop5_56-rel_N_Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Nop" evidence="3">
        <location>
            <begin position="252"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="365"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="373"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="417"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ05929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="428" mass="48314" checksum="1A33E93EC97DE164" modified="2017-09-27" version="1">MKAYIAENVRGVYAFDEDGNLIATRSFAGKPEISLDRLLKGEPVDELTELMDELGERGYTEFVVEDTELARNLKELGYSATAEFPNVAGEKLRSNPEEFLGEKWFDEYFSVGVALTRLRIQEQSGARDKMIIQAIEALDDIDKVTNLLVSRLREWYGLHFPELDEILPKHEQYVAFVSTVGAKENATEGRLKSLGLPEGKIEKILKAAEKSMGAPLGKFDEDIIKKLASEINDLYKLRKQIEDYLETAMDEVAPNLKALVGAKLAARLMSLAGGLRELAMMPASTIQVLGAEKALFRHLRSGAKPPKHGVIFQYPAINRSPWWQRGKIARALAGKLAIAARVDYFSGEYIAEELKQELEQRIQEIKQKYPNPPKRKAKPEKKKKEKFKGKGGKHEKGRKFEKKEKAKGKKEKGGKDKKPKKKKSKNKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P589</accession>
    <name>A0A218P589_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1884 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_00580</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853198.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P589"/>
    <dbReference type="GeneID" id="33314720"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00580"/>
    <dbReference type="OrthoDB" id="85203at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.10">
        <property type="entry name" value="hypothetical protein PF0899 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014418">
        <property type="entry name" value="ENCP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036216">
        <property type="entry name" value="ENCP4_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041190">
        <property type="entry name" value="encap_f4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08967">
        <property type="entry name" value="ENCP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004604">
        <property type="entry name" value="UCP004604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111057">
        <property type="entry name" value="Hypothetical protein PF0899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ05935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="93" mass="10243" checksum="E7A15B7C16BD8F5F" modified="2017-09-27" version="1">MRGDLIRVLSTAEEKANELKLDGYEPDVILLGKEAYDFVREQANEEFGGEEEVFELSGLKVRVVEELGKDAVVIDSKTLGMGPGGAKRFKVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5A2</accession>
    <name>A0A218P5A2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N(4)-bis(aminopropyl)spermidine synthase</fullName>
            <ecNumber evidence="1">2.5.1.128</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Branched-chain polyamine synthase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">bpsA</name>
        <name evidence="3" type="ORF">A3L08_00720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of branched-chain polyamines, which support the growth of thermophiles under high-temperature conditions. Catalyzes the sequential condensation of spermidine with the aminopropyl groups of decarboxylated S-adenosylmethionines to produce N(4)-bis(aminopropyl)spermidine via N(4)-aminopropylspermidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 S-adenosyl 3-(methylsulfanyl)propylamine + spermidine = N(4)-bis(aminopropyl)spermidine + 2 S-methyl-5'-thioadenosine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:44132"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:82771"/>
            <dbReference type="EC" id="2.5.1.128"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amine and polyamine biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the branched-chain polyamine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.128" evidence="1"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853220.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5A2"/>
    <dbReference type="GeneID" id="33314748"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00720"/>
    <dbReference type="OrthoDB" id="358909at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006596">
        <property type="term" value="P:polyamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01947">
        <property type="entry name" value="Aminopropyltransf_BpsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014435">
        <property type="entry name" value="BpsA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002723">
        <property type="entry name" value="BpsA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051720">
        <property type="entry name" value="rRNA_MeTrfase/Polyamine_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23290">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23290:SF0">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01861">
        <property type="entry name" value="BpsA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005895">
        <property type="entry name" value="UCP005895_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N(4)-bis(aminopropyl)spermidine synthase C-terminal" evidence="2">
        <location>
            <begin position="106"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ05958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="351" mass="40240" checksum="8F9FD838891CB59D" modified="2017-09-27" version="1">MREIVERVKEKTSIPVYERTIENVLSAIQASGDVWRIVDLSEEPLPLVVAVITALHEMGYITFDDPNVVLTESGKRLVEKYGIGTRRDYTCSHCQGKTVELSAFSDLLEQFKEIVKDRPQPKHDFDQAYVTPETTVARIALMHTRGDLENKEVFVLGDDDLTSIALMLSGLPKRIAVLDIDERLVKFIEKTADELGYSNIEMFTFDLREPLPDYALHKFDTFITDPPETVHAIRSFVGRGIATLKGPGCAGYFGITRRESSLDKWRDIQRILLNEFGVVITDIIRNFNEYVNWGYEEETRAWKLLPVKVKPSYNWYKSYMFRIQTLEGSKGFEDRITVGDELYNDEEASTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5E9</accession>
    <name>A0A218P5E9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Iron ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_01005</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853271.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5E9"/>
    <dbReference type="GeneID" id="33314805"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01005"/>
    <dbReference type="OrthoDB" id="24644at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03214">
        <property type="entry name" value="ABC_Iron-Siderophores_B12_Hemin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000134">
        <property type="entry name" value="Iron-enterobactin ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42794:SF2">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42794">
        <property type="entry name" value="HEMIN IMPORT ATP-BINDING PROTEIN HMUV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="242" mass="27217" checksum="236BD40496A707AE" modified="2017-09-27" version="1">MKLEVDVSFSYGEREVLKNIRFTAERGELLAIIGPNGAGKSTLLKAMVGILTPRGTVKLDGRDLIRMKPRERAKLITYVPQSSFPEFAFTIEEFAEMGTYATRGDVESALKRVGLWERRREPVTNLSGGEYQLVLIARALAQGSHVILLDEPTSHLDINHALEVMELLKELKEEKIVIAVLHDLNLALRYADRLILLHRGGKYWEGHPEELGSKVLEKVYGVRARIAEVDGHRVIVPELTKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5F3</accession>
    <name>A0A218P5F3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">A3L08_00845</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853243.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5F3"/>
    <dbReference type="GeneID" id="33314773"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00845"/>
    <dbReference type="OrthoDB" id="50101at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05144">
        <property type="entry name" value="RIO2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000647">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000052">
        <property type="entry name" value="Serine/threonine-protein kinase RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030484">
        <property type="entry name" value="Rio2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015285">
        <property type="entry name" value="RIO2_wHTH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45852">
        <property type="entry name" value="SER/THR-PROTEIN KINASE RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45852:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09202">
        <property type="entry name" value="Rio2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="98"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ05981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="312" mass="35740" checksum="78014A9C79F41C10" modified="2017-09-27" version="1">MVSKLLALDAYPNLRDLDFRILRGVELNMRRYKWVPLEDIARFAKVDIETASFRLGKLDNWGLVVRRSDIGYIGYQLTIHGYDTLAIRALSKKGIIEAISTTQIGVGKDADVYVGVTPSGGKVAVKFNRIGGRTASRKAGYHGHVFQDKRHTSWLYVSRLIARKEYEALTLLSPIARVPRPIAWNRHVVVMEFINGTELAELRDTDLTREEAGEILSKILDEYLKIVRFGIVHSDLSQFNVVLTDDGSILIIDWAQYITTDRPESYELLKRDLTVLLNAFKRRWGVKRELEDVWPAFEEAWHESRGESHERG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5H1</accession>
    <name>A0A218P5H1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853287.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5H1"/>
    <dbReference type="GeneID" id="33314823"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01095"/>
    <dbReference type="OrthoDB" id="85651at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="268" mass="31417" checksum="B8262C082679CBDF" modified="2017-09-27" version="1">MFTVDEISELVREIRRENGFPDSPFRIDEVRYDPEGDKLFIIAHDRTDKSVLIGNSFVIGKLRERLGVKQLTVYSNLDLEIKRRKLEKAERLVEDTELEFLLPIIEAEKRFPPRKWPEVKGDVKTLVFLSFNAKALIGFAQRLNLPHEAVGLKYTFPKLQYEPVEGEPRELLSPNEEKLVELAKERGAKLVLADFPFGLRWSEGIALLNPFRFLHIGFFELKYLFGFEFPTMIDYDALIRFVAELTYEGLMESTDGANVIWRYWRRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5H9</accession>
    <name>A0A218P5H9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853294.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5H9"/>
    <dbReference type="GeneID" id="33314830"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01130"/>
    <dbReference type="OrthoDB" id="99025at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="236" mass="25580" checksum="063640FEF51D7C9F" modified="2017-09-27" version="1">MRKFIFALLAVTLLVTVAGCENPDTNVSTEKTLTINEVTVHYSGDVSLSQAKAVLNFVRDNFQINGETDVYVSKSGDSYTVTVTTPYESAGDIDKETAFYVKIMASKMSQDVFNGAKVTLKLLNGDEEEIFSAESKYAYIESNGITVWYAGVSEDDAQKVLDYAVSVAGSGPWDIFIDGSNPYTIGAMSSFNSADEIGDAESIYQEMAADLSERLGGNLVLRVLNPSGEEIARFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5I4</accession>
    <name>A0A218P5I4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">eCIS core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853273.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5I4"/>
    <dbReference type="GeneID" id="33314807"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01015"/>
    <dbReference type="OrthoDB" id="85977at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025295">
        <property type="entry name" value="eCIS_core_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13699">
        <property type="entry name" value="eCIS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eCIS core" evidence="1">
        <location>
            <begin position="106"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="363" mass="41318" checksum="12640B271C01CEEB" modified="2017-09-27" version="1">MRKPHDRILALVLLGVLLLSLYSAVRLNTHPNAVLDQVNLILSQVQEIRGLNFKEPPQIVVITRAKALEMWKPGKPDLERLHREELVYKMTLLLPPDYDYIKEEHERSAGWIAATVGNTIYIIRENFMGDLDTARRTVAHESVHVLQKQWFDAKYGADTFDGTLAVQALVEGDADLVADIYCKRNGIPIHKIRSLSGDPLTDVHIFPYVFGDSFVRYLYERGNWTLVNGAYTRYPRTTLEVMEPELYLKNFTPVNVALSPPNGSQVLRDDRMGAFYVYVLLKDVAKLDNETAWNVSTAWLGDRLILARTSDGYVLLWGITFSNEGAAEEFTETLKKLAQDNSYASREIYVDGKTVLLKAVRRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5I8</accession>
    <name>A0A218P5I8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853304.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5I8"/>
    <dbReference type="GeneID" id="33314843"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01195"/>
    <dbReference type="OrthoDB" id="102566at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="84" mass="9242" checksum="94A8E471AA57DF47" modified="2017-09-27" version="1">MMEILLVAVTIALSLVLAGISSVAYRKSHLRPALYLLIAFLIFALKKVIEALKLASWIEKDVGLVTGVLEAAVLVLLLLSLWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5J1</accession>
    <name>A0A218P5J1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5J1"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01070"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="68" mass="7742" checksum="E9F55679B01CA7F5" modified="2017-09-27" version="1">MSQRENVLPGMKSWRGSVRKGTGRRQGQRAKPMNPKRRNIKKWASLSPFSTGDVGTAGKRFKNSRRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5K4</accession>
    <name>A0A218P5K4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01125</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853293.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5K4"/>
    <dbReference type="GeneID" id="33314829"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01125"/>
    <dbReference type="OrthoDB" id="97584at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="152" mass="17191" checksum="D4D987E9E460E30F" modified="2017-09-27" version="1">MEKVLHVVGNLDYKRGFMRESRASLVITNERMIVVFLTKELAKEIKKEAGGGIKGFLKGVAGAYGHWNKYYEMSPEEILKESPENFEIPLREVKEVKIKSGDSESGKRGEIEVKGGNNYKFYEGDATIHIRELKKTLEGLGIKVKAPRFSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5L2</accession>
    <name>A0A218P5L2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH arsR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853303.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5L2"/>
    <dbReference type="GeneID" id="33314842"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01190"/>
    <dbReference type="OrthoDB" id="28610at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012318">
        <property type="entry name" value="HTH_CRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056504">
        <property type="entry name" value="HTH_HVO_0163_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033788">
        <property type="entry name" value="HTH_metalloreg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36216:SF1">
        <property type="entry name" value="HTH ARSR-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36216">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, TRMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24266">
        <property type="entry name" value="HTH_HVO_0163_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00419">
        <property type="entry name" value="HTH_CRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="58"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="169" mass="19500" checksum="39FF7E7F0631E6CD" modified="2017-09-27" version="1">MERRSEIFDAIRAKPGITFRELARELGVGIGDLQYHLWKLEKEGRIFSRRAGRRRYIFPRGLEEDAQRLLIAISTDTRRRILLLLMEGPMGQKELAETLGLSQPTVSYHMSELVRLGIVKAERSGKNVVYTLSYDPETVVRLIREYRPSLWERLADGLIDLLTSVGDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5N9</accession>
    <name>A0A218P5N9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853381.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5N9"/>
    <dbReference type="GeneID" id="33314929"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01620"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="114" mass="12614" checksum="E9F736818C6A21F5" modified="2017-09-27" version="1">MAGQEAGVLGSKIKQKVHEILFIPKDDEASLSVILEVYSRRVQHGVNEPLAWFNVKVCPDGLLVEDTPLRKTLAGVSLIIMMSAMAPLLFFRSIIFVEIEMEPLTQYAVLPSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5P1</accession>
    <name>A0A218P5P1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Universal stress protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_01405</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853343.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5P1"/>
    <dbReference type="GeneID" id="33314886"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01405"/>
    <dbReference type="OrthoDB" id="107030at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF26">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN MJ0577"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="152"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="278" mass="31142" checksum="855FFD7B0D2E3DE3" modified="2017-09-27" version="1">MFEKVLYPTDFSEVSLHALRHCLPKLVEQGVKELHLIHVIDITVAEFEAFELEEIYREKLAELAEELKEMGAEVEVIVKIGIPSMEIAETAEENNIDLVVIPSVGENIWRRMFVGSTASNLARATKRPVLLLKYVQKDDKFEFSGDCSEVFKRPLVALDFSECSIKILKTVKEFEELIEGGILLHSVDYGNVDELEHNIEAARKNLEKSAKGIRAKFETEVMVGPASQAIIGMALAKKASMIVIGKKGRNFLKDLILGSTAERVMRDAKMPVLLVPCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5P2</accession>
    <name>A0A218P5P2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">CidB/LrgB family autolysis modulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_01555</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853369.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5P2"/>
    <dbReference type="GeneID" id="33314916"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01555"/>
    <dbReference type="OrthoDB" id="141102at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007300">
        <property type="entry name" value="CidB/LrgB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005261">
        <property type="entry name" value="YohK-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00659">
        <property type="entry name" value="CidB/LrgB family autolysis modulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30249:SF0">
        <property type="entry name" value="PLASTIDAL GLYCOLATE_GLYCERATE TRANSLOCATOR 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30249">
        <property type="entry name" value="PUTATIVE SEROTONIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04172">
        <property type="entry name" value="LrgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="224" mass="23417" checksum="E53D9F2A84CA770E" modified="2017-09-27" version="1">MNPYGIALTLVVFYAFSELHRRKRAFYTNPVLLSITAIAALLWAGGYSYESYMESAVILKFLLGPAVVSLAVPVYKGRETIKAYAREITAGIVIGGTVAILSAFYTAKLFGGAEEVLLSMTPKSVTTAIAIGVSEKIGGIPALTAVLVVLTGILGNAVGVELLNVAGIKDRIARGLAMGVTSHGLGTARIILDDELAGAVSGLAMALNGVFTSLLLPYLIEVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5P6</accession>
    <name>A0A218P5P6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pantoate kinase</fullName>
            <shortName evidence="1">PoK</shortName>
            <ecNumber evidence="1">2.7.1.169</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_01515</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Phosphorylates (R)-pantoate to form (R)-4-phosphopantoate in the CoA biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-pantoate + ATP = (R)-4-phosphopantoate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28246"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61294"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.169"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GHMP kinase family. PoK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.169" evidence="1"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853362.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5P6"/>
    <dbReference type="GeneID" id="33314908"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01515"/>
    <dbReference type="OrthoDB" id="85822at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02223">
        <property type="entry name" value="Pantoate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053616">
        <property type="entry name" value="GHMP_kinase_PoK-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012043">
        <property type="entry name" value="PoK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041122">
        <property type="entry name" value="panto_kin_Thplsm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42282:SF1">
        <property type="entry name" value="PANTOATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42282">
        <property type="entry name" value="PANTOATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016896">
        <property type="entry name" value="GHMP_arc_MJ0969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1 3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="2">
        <location>
            <begin position="85"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="300" mass="32600" checksum="91D416A0C82A4266" modified="2017-09-27" version="1">MLVRAFIPAHITAFFVPVFNEDPLKAGSLGAGINLSKGTNVFVSIEKETLEKHIHIAFNGEPVKREEASISYSVAEKLVPEEFIGEVEVWQYFDFPNGYGFGNSAGGALGTALALSYRFGGTWLRAAQIAHEAEVIHRGGLGDVIAQLAGGIEVRVKAGGPGYGVVDNLFFEDYKVLVVPLGRLSTREVLDGDVVTAIEAEGRKALEALLKEPTPEKLMITARDFAETTGLLTGELLELAKELDKVLKLPSSMIMLGKGLFALAREEELENAKTLLAELEVPYDIAEIYVERPKVGRWLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5R7</accession>
    <name>A0A218P5R7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carbohydrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01720</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853398.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5R7"/>
    <dbReference type="GeneID" id="33314949"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01720"/>
    <dbReference type="OrthoDB" id="23670at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02222">
        <property type="entry name" value="cupin_TM1459-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37694">
        <property type="entry name" value="SLR8022 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37694:SF1">
        <property type="entry name" value="SLR8022 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-2" evidence="1">
        <location>
            <begin position="38"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="118" mass="13394" checksum="EF9E500F68799F61" modified="2017-09-27" version="1">MFVGHYKDVPEKDTGFEGVTIRWLVSPKLGAKNFAMRYFVLKKGAEIPMHQHDWEHEIFIVRGEGIITGGDKEVHVKAGNFLYVPPNEPHGYKATGETLEFLCIIPAKKEAIPEDEWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5T1</accession>
    <name>A0A218P5T1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ABC transmembrane type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_01685</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853391.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5T1"/>
    <dbReference type="GeneID" id="33314942"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01685"/>
    <dbReference type="OrthoDB" id="312811at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050366">
        <property type="entry name" value="BP-dependent_transpt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43386:SF1">
        <property type="entry name" value="D,D-DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DDPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43386">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN APPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="221"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="361"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="185"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="393" mass="43209" checksum="38C2AF14751B7D7E" modified="2017-09-27" version="1">MMINRKGVLMIAVFIAFLVGAHFSVSPSDMEHWDDRLYWKDYPKNALPVWHPGADMGTLRLEGQGEIVVSVEALPTNLVLSKATLVITRPDGRSIRLSGSGSVNSNTTLVAEAKAFARELGLSETALILYQPTQLLFLSENETLLKGDYIFRVEAGRAILFGNAYGPLGTDGYGRDLWVGFVKSTWGTVVLTVLTVSFVILIGLPFGVISGYHRNVLGDAVTVIVDAFNSIPAIPLVLVMIYAISRVGAYTKLVVPDWLIGIILSLFFFSQYSNSIRGIVENEKVKEYMAASKSIGASDSYVVFRHILPIVLSYTVPYVTMLFPRVMAFISVLGLFNIIPGNNWGSYIAEALKQGALMGRYWWWVMAPVIVIAFLAVAVSNLFEYAETSGVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5U0</accession>
    <name>A0A218P5U0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">tRNA-t(6)A37 methylthiotransferase</fullName>
            <ecNumber evidence="11">2.8.4.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">A3L08_01725</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:37075"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="Rhea" id="RHEA-COMP:11092"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:74420"/>
            <dbReference type="EC" id="2.8.4.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="11">Binds 1 or 2 [4Fe-4S] cluster. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the methylthiotransferase family. CDKAL1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.5" evidence="11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853399.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5U0"/>
    <dbReference type="GeneID" id="33314950"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01725"/>
    <dbReference type="OrthoDB" id="372134at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035598">
        <property type="term" value="F:tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12160:FF:000017">
        <property type="entry name" value="Threonylcarbamoyladenosine tRNA methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.30.20:FF:000002">
        <property type="entry name" value="threonylcarbamoyladenosine tRNA methylthiotransferase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="entry name" value="Methylthiotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006466">
        <property type="entry name" value="MiaB-like_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01578">
        <property type="entry name" value="MiaB-like-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00089">
        <property type="entry name" value="MiaB/RimO family radical SAM methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11918">
        <property type="entry name" value="RADICAL SAM PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11918:SF45">
        <property type="entry name" value="THREONYLCARBAMOYLADENOSINE TRNA METHYLTHIOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01061">
        <property type="entry name" value="methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 11 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="MTTase N-terminal" evidence="13">
        <location>
            <begin position="2"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="14">
        <location>
            <begin position="134"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="12">
        <location>
            <begin position="366"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ASJ06135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="425" mass="47879" checksum="E9679DDC0EF62219" modified="2017-09-27" version="1">MVRVHVESYGCTRNKADGEMMEAILLRAGYELVETPESADYVVVNTCAVKDPTEKHMKERIKELLDSGKRVIVTGCLTHVNPDVIDPRVSGILGVKSIDRIAEAVEIAEGGGKLVSVEGWRERNIDKLELPRLWKSGVAFVVPISEGCLNGCTYCATRFARGVLKSYKPELIVKWVKEALSRGYKEIQLSSEDTGCYGFDIGTNLAKLLDEITAIEGEFRVRVGMMNPNHAIKILDELVEAYQSEKVYKFLHLPVQSGDNDVLKRMGRTYTVEEFEEIVQIFRKKVRDLNLNTDIIVGFPGETDEAFENTVELVKRVRPDKINVSRYSPRPGTIAARWKQLPGWKVKERSRELHRLRLGIAYEINQSYVGKEVEVLVHGSGKKGGVEGRTFNYKDIILDSGEPGEFVRVRVDWAGSTYLRGTLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Y6</accession>
    <name>A0A218P5Y6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_02005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853450.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5Y6"/>
    <dbReference type="GeneID" id="33315006"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02005"/>
    <dbReference type="OrthoDB" id="85492at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="227" mass="26469" checksum="6B27AB4B18C8F350" modified="2017-09-27" version="1">MQGFKLLPSVAYLRVQRQAFVGYSMPLAGWVGEYLINYGKLPRPNFLSRAMGKLGFSLAGEERDERYVTQFFTKGSVSISASWDMEKENLFLQLIPLRSRLSRGLTVRTEYLEFYDQYVVSIEPTNKLPPGIRGIGINALILEDFYPVETPYWGMLHEDWESELNLVVMRDEVYDKLEREEYRCPVCFSPLSEEKGILKCTHCGFTYTPEHDFERVMEEFTVEEFAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P631</accession>
    <name>A0A218P631_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853498.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P631"/>
    <dbReference type="GeneID" id="33315058"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02265"/>
    <dbReference type="OrthoDB" id="85879at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014509">
        <property type="entry name" value="YjdF-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09997">
        <property type="entry name" value="DUF2238"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="206" mass="23523" checksum="9AF6CC77D1158F96" modified="2017-09-27" version="1">MVDIGRERSIIALSIFVVFLGFMTALFNGREDHVYRTLVAMIGLSIPLILPKKLPEPPAKLRPFLAPLYNERTMALLAVFIAVHVSLVNVPFTHYDLFHKDWRNADMISHFLGGLTVWLIVVEILSELDRIGIRLSKREAVLYGFLIFYALSTGWEVAEKLSETEISFIRESLANKLRDLLMDTLGALFGLWLVRREGYPFSLGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P653</accession>
    <name>A0A218P653_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Alanine glycine permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_02455</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the alanine or glycine:cation symporter (AGCS) (TC 2.A.25) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853531.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P653"/>
    <dbReference type="GeneID" id="33315096"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02455"/>
    <dbReference type="OrthoDB" id="77152at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005283">
        <property type="term" value="F:amino acid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001463">
        <property type="entry name" value="Na/Ala_symport"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00835">
        <property type="entry name" value="agcS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30330">
        <property type="entry name" value="AGSS FAMILY TRANSPORTER, SODIUM-ALANINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30330:SF3">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, LRP FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01235">
        <property type="entry name" value="Na_Ala_symp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00175">
        <property type="entry name" value="NAALASMPORT"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00873">
        <property type="entry name" value="NA_ALANINE_SYMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0769">Symport</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="153"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="307"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="358"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="451"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="484"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="516" mass="55470" checksum="B992386C351E6192" modified="2017-09-27" version="1">MSAIVDFINWLDGEVWGIPMIVLLLGTGLLLTAILKAIQFRRLGWAIRFTLFEGRKKTGEGDITPFQALMATISGTVGIGNIAGVATAIHFGGPGALFWMWITALVGMATRYSEGLLGVAFRDKLPDGTQIGGTFNFLEKGFAMENIPKTGKYLAAIFTLLFAVFIGYDATQISGATQIGAIIVAILFAILGLFLLKDDAYPTLGKVLAILFALFASIAAFGIGNMTQSNSVADAIRTAFNVPMWVTGLVLAVLTFIVVIGGIKRIGEVAEMLVPFMAIVYFLFAIGVWIKFAGKLPSAFALIVKDAFTGQAVAGGAIGQVVLWGVKRGLFSNEAGLGTATIAHASAKTDHPSRQAHVAMLGPFIDTLIICTLTGVSIVVTGVYTNPDLNGAPLTQAAFAAAFGHAGEIMVAIGIILFAYSTILAWSFYGRQNVMYLAKWLEQDPEKFARLYPKLHMIYNLLFVVFIYIGAVTKLETVWNFSDMMNGLMAIPNLIGLLVLSWYVKKKTDEFVAANP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P683</accession>
    <name>A0A218P683_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">A-type ATP synthase subunit F</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">atpF</name>
        <name evidence="5" type="ORF">A3L08_02595</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the V-ATPase F subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853558.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P683"/>
    <dbReference type="GeneID" id="33315125"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02595"/>
    <dbReference type="OrthoDB" id="24971at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10580">
        <property type="entry name" value="ATPase, V1 complex, subunit F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00312">
        <property type="entry name" value="ATP_synth_F_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008218">
        <property type="entry name" value="ATPase_V1-cplx_f_g_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022944">
        <property type="entry name" value="ATPase_V1-cplx_fsu_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036906">
        <property type="entry name" value="ATPase_V1_fsu_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003047">
        <property type="entry name" value="PRK03957.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01990">
        <property type="entry name" value="ATP-synt_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159468">
        <property type="entry name" value="AtpF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="3 4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="102" mass="11454" checksum="961DCABC301FE895" modified="2017-09-27" version="1">MKIVVLGDRDTALGFKLAGAHEVYAFDDTPLEIERLKNKLKELVERDDMGIILITEGFARRVELPDVTLPIILQVPDKSGSKLGEETIKEIVRRAIGVELKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6B1</accession>
    <name>A0A218P6B1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">NH(3)-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="8 10">6.3.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nadE</name>
        <name evidence="12" type="ORF">A3L08_02685</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>deamido-NAD(+) + NH4(+) + ATP = AMP + diphosphate + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21188"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the NAD synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.5" evidence="8 10"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853576.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6B1"/>
    <dbReference type="GeneID" id="33315143"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02685"/>
    <dbReference type="OrthoDB" id="39312at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00333"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008795">
        <property type="term" value="F:NAD+ synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000106">
        <property type="entry name" value="Glutamine-dependent NAD(+) synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00193">
        <property type="entry name" value="NadE_ammonia_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022926">
        <property type="entry name" value="NH(3)-dep_NAD(+)_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010587">
        <property type="entry name" value="PRK13980.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090:SF9">
        <property type="entry name" value="GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8 9" id="KW-0520">NAD</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD/GMP synthase" evidence="11">
        <location>
            <begin position="10"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="32"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <begin position="244"/>
            <end position="245"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="254" mass="28752" checksum="0E6939A8F45FDB22" modified="2017-09-27" version="1">MRELEYPKVVEEIISFIRENVEKAGVEGVVIGISGGIDSATVAYLAARALGKERVLGLIMPYYENRDVEDAKLVCENLGIECREISIRPIVDSLVAQLGFQPDKRSLGNIMSRTRMVLLYAHANQLNRLVLGTSNRSEFLVGYFTKWGDGASDYAPLINLYKTEVWEVAKLLGVPQRIIEKKPTAGLWEGQTDEDELGISYRLLDEILWRLVDLKMPKDKIAEELGTDIERVEYVERLVKSSEHKRRLPLGPTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6C5</accession>
    <name>A0A218P6C5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Alanyl-tRNA editing protein AlaX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_02615</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853562.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6C5"/>
    <dbReference type="GeneID" id="33315129"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02615"/>
    <dbReference type="OrthoDB" id="11392at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000026">
        <property type="entry name" value="Threonyl/alanyl tRNA synthetase SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051335">
        <property type="entry name" value="Alanyl-tRNA_Editing_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43462">
        <property type="entry name" value="ALANYL-TRNA EDITING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43462:SF1">
        <property type="entry name" value="ALANYL-TRNA EDITING PROTEIN AARSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alanyl-transfer RNA synthetases family profile" evidence="6">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="263"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="408" mass="46252" checksum="73AD1E7B47DF61B3" modified="2017-09-27" version="1">MTEKLFYRNPYLWEAEGTLLAVEKGGKGVRILLDRTIFYPEGGGQPSDRGVIAGEDFRILVEKVHGKDEVWHEGKLEGRLPEPGEPVRMVVDAEWRYENMRQHTGQHILSAVFKELYDANTTGFQIFEGYNKIEIDYPGELDWDMILEVERKANEVVWRNLPVQVEVYDELPKELKENLRKELSEKVKPPIRIVSIPGVDVIPCGGTHVKSTGEVGFIKILNFYRKSRKLWRVEFVCGNRALKKLNEILDDYWSALDRMPNKNPPLAERVKELKAEVEGLEEEKDSLRKELWEWKARALLNEAQEIGGIGVISHLEKAPLKDAQAFVVHLVDKNPNTIALIAGENYVIFAKNKEIEGISMNDLLKEVLSKTGGGGGGSEVLARGGGFKVRPEEVLELAFEKLRGELSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6E1</accession>
    <name>A0A218P6E1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Aldehyde ferredoxin oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">A3L08_02900</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>tungstopterin</name>
            <dbReference type="ChEBI" id="CHEBI:30402"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AOR/FOR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853615.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6E1"/>
    <dbReference type="GeneID" id="33315186"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02900"/>
    <dbReference type="OrthoDB" id="30771at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.569.10">
        <property type="entry name" value="Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.599.10">
        <property type="entry name" value="Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.9.10">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013984">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013985">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013983">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036503">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001203">
        <property type="entry name" value="OxRdtase_Ald_Fedxn_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036021">
        <property type="entry name" value="Tungsten_al_ferr_oxy-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051919">
        <property type="entry name" value="W-dependent_AOR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30038">
        <property type="entry name" value="ALDEHYDE FERREDOXIN OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30038:SF7">
        <property type="entry name" value="TUNGSTEN-CONTAINING GLYCERALDEHYDE-3-PHOSPHATE:FERREDOXIN OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01314">
        <property type="entry name" value="AFOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02730">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00790">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48310">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, C-terminal domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56228">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0826">Tungsten</keyword>
    <feature type="domain" description="Aldehyde ferredoxin oxidoreductase N-terminal" evidence="11">
        <location>
            <begin position="4"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="551"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="623" mass="69527" checksum="BBA721C0EC45D523" modified="2017-09-27" version="1">MKGYAGKLLDVNLSTGDVKTVELDEDMLRFYGGRGLGTYILWRELGERWESIDPLGEENLLLILTGPLTGYYPGIKTAVVSKSPESNGVVGSVLSSEVGIELKSAGYDGIIIRGKASEPVYLFINDDEVEIRDASKYWGMGGLELHKTLLKEVHEELRKKGKLKGVPKEPAMMYIGRGGEEKVRFAAIMSKLMHAAGYGGFGAVMGSKNLKAVLVKGNGPLPEVHDPERFKSLLREFQRELLTLTTFRQWGTGAGGYSVGKDRSSQPIRNWQEEYHDDERISVVNFELKAWVKKYWADYGCPVNCMKISYLRYGPYKGAITDAPDYELMAYMGTNLGIFEPEKIVYLSYLVDELGLDGINAGNVLGFAAELYQRGILTEEDLGFKLEWGDEKAFARLLELIVRGEGIGKVLAEGTYRAAKRISEMKGVDAMRYAVHVKGIGVGAHGVRSGLDYTRDISYAVSVQGGDHTATAGLPARSYEGEMVNAFYDSAVICMFTTRPGFERIIEFGNAITGFELTPEKWFNEMGLRIIHLQRILLLLGGPDVYWDPRRDDDNPPRFYEPLPSGPVKGSAPTKEEIQEKARQYYEEIGYDEHGIPREDVLEELGLGEAKREVKRIRKRLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6E4</accession>
    <name>A0A218P6E4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Dipeptide/oligopeptide/nickel ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_02670</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853573.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6E4"/>
    <dbReference type="GeneID" id="33315140"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02670"/>
    <dbReference type="OrthoDB" id="18209at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000016">
        <property type="entry name" value="Oligopeptide ABC transporter ATP-binding component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43067:SF3">
        <property type="entry name" value="MALTOSE ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43067">
        <property type="entry name" value="OLIGOPEPTIDE/DIPEPTIDE ABC TRANSPORTER, ATPASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="319" mass="35647" checksum="359EC1FF925AF021" modified="2017-09-27" version="1">MVKNVLEVKDLKMYYFTNKGVVKAVDNISFNLRKGEVLGLAGESGCGKSSLGFTLLGMPTPPGKIVSGSIKIDGREIVGLPEDVLRKEIRWQKISMIFQGAMNALNPVYTVGYQMTEPLLLHKGMNKEEALDRAQKYLELVGLDPEIVYRYPHELSGGMKQRVIIATALLLEPDVVIADEPTTALDVVVQAQIINLLKKLKKELGLSMIFITHDLSILAEISDRVAIMYAGKIVEIGDSEKIYYEPAHPYTQKLLAAIPRLHEDVEKLEFIPGQPPNLINPPKGCRFSPRCPYVMNVCTEQEPELKEVDKDHYAACWLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6F7</accession>
    <name>A0A218P6F7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glucose-1-phosphate thymidylyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_02960</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853626.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6F7"/>
    <dbReference type="GeneID" id="33315198"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02960"/>
    <dbReference type="OrthoDB" id="15372at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="2">
        <location>
            <begin position="2"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="3">
        <location>
            <begin position="249"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="331" mass="37494" checksum="4BAB85B1B4E2420C" modified="2017-09-27" version="1">MKVLIMAGGYATRLWPITKDNPKALLPLGEKTILDYIMEKVNELGLDAYISTNRFFEAHFKPYAEKYGVGLIVEDTLHEEEKLGTIGAMKRAVEELGLDDYLIIAGDNLFSFSLSDFLRAYDGRTLIAVYDVGDPELAKRYGVVVLEGDKVISFQEKPVQPQSTLISTGVYVFPKAVMERVDEYLSNGNRDSPGYFLQWLLEKGEPIKAYRFSDYWYDIGSADSYLEALKTLLKESHIEEIQISPYSKIIPPVVIRRGAKILGRSIIGPYAYIGEDCVIENSDVSDSIIFRRTIIRNSTIWRSIIDEKCEIRNLELRKSLVGGHAKIQRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6G0</accession>
    <name>A0A218P6G0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853635.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6G0"/>
    <dbReference type="GeneID" id="33315208"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03010"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="304" mass="32923" checksum="C5EAC3938879FF2A" modified="2017-09-27" version="1">MKRMFALLMGLLVLGGMMGMVSAFTPTIDGNDSDWGSLDLLLLDPNSWNTPSSLQEKGLDSQHLQYIWMDTSGDAIDSVDAADVKYIRVTANSSYVYFYIKFTGQLTLGGKNPNAQNIPAVAIIIDSTDGGNKTFGGQVLWGADVKVSDSDPYAWDYLIRVDLNQFSTGGTYDNPAGVDVLDIYGDNVLYDDSNNLVYGDYSIGIYTGDTTTGGGIELKIPKGALGLSDQQFGFYMDFLTVLQTANSGSVQVEDAMDPLSSGSNTLSNFQYADITQVPFFSDSIAMGAVAILLALGAFWFFRRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6L2</accession>
    <name>A0A218P6L2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF2101 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853683.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6L2"/>
    <dbReference type="GeneID" id="33315266"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03300"/>
    <dbReference type="OrthoDB" id="86267at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018663">
        <property type="entry name" value="DUF2101_membrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09874">
        <property type="entry name" value="DUF2101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="28839" checksum="F00D333187597EB6" modified="2017-09-27" version="1">MSVEEILYRVGDAVYSIGDRISEFLKPSPSEQPPDFKYLKKVIKKPLSPHEFLSLKLQISFLLYLGIGLILLFLRANPLWILTLAVINFLYVRHLTTRNRSFFIDVEPYRFFYYSITWLTFAAFAGYDAVRRLATSVYYYYGYLALVVAVILAFRWYFKQRYGRDYTYGTVEEIKGEAVRVFVHDDIAANVKPGYYWVDAVEDLEVGRVVKLIVEDKKLRGAVPVRIIEIYLDDQSSQSSTEPKNATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6P7</accession>
    <name>A0A218P6P7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF432 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03540</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853724.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6P7"/>
    <dbReference type="GeneID" id="33315313"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03540"/>
    <dbReference type="OrthoDB" id="59763at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007366">
        <property type="entry name" value="DUF432"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04254">
        <property type="entry name" value="DUF432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019202">
        <property type="entry name" value="UCP019202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="23930" checksum="5C87FB2F07579235" modified="2017-09-27" version="1">MFGEHELKTQFIKIADKKVHLLEDKGGIVRYRRDEVEKIIKSNGEKLKILPSPAVGYGVRLLMVKFREPVVVPPQGSLIGYIEAPVEIDVKVGELSIDHFIVGKEKYALYGTLEAGVICRYHVSSFHAEEPESLGVVKLIVSNPSSEWKSLEKVIIPVKGSSMYYTEDKAYYPLLVVTIKNHIPEVNNTGKPPKEGLNSLGNQLSLPNFLMRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6R0</accession>
    <name>A0A218P6R0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_232461762.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6R0"/>
    <dbReference type="GeneID" id="33315315"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03550"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="391" mass="42294" checksum="FD9C26080B74540A" modified="2017-09-27" version="1">MKKVVTVMLILTLLGMVAASGCLDGGEESSKTSPTSSTTAPTSSLPMTSKPSETTTSQAPQSWEMELVWNVSTSGIPLLDMSPDGSLSAVIDWNNADLYLVKPSGESVTFDLQGDDAVEPVVVGVVVRDREAHVLADYAEFAGVRIYSWEGQTGEERHGWAGAVADSIAISPSGNHLCYLITTGATTQELYCDGVKTTIENAGGYNMVDVSDTGLVAVGSGGEAGDVLIFKNGSRVMTLSPDSHMVVLYGDRIIGQFEGELRVLDAEGNVLARSGQYCLRWYSLLIPSVRATEKYLLWTDEFEGTRVLTWNMTEVKSLEGFMRFANENFVVTVKGGVIHCYSLRDFHEVFSVKVPGESLGYVRLSDDGRVLLVSGEFGSFWLYVKGLTLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6R2</accession>
    <name>A0A218P6R2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleotide-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03565</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853727.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6R2"/>
    <dbReference type="GeneID" id="33315318"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03565"/>
    <dbReference type="OrthoDB" id="15280at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09879">
        <property type="entry name" value="PIN_VapC_AF0591-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041120">
        <property type="entry name" value="PIN_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12416">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN UTP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18477">
        <property type="entry name" value="PIN_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="6"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="137" mass="15934" checksum="E38DA1C258D5EFFC" modified="2017-09-27" version="1">MGKKEWLVIPDTNFLLVPGQFGVDIIGELNRILDVRFRILIPNVVLQELEVIERKSKGKDLMAIRMAKKLAERFERVDIGEFGKRPIDDQIFDFAVKNERVIVCTNDKGLKRRLRERGVPVVYLRSKKILELEGMLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6T1</accession>
    <name>A0A218P6T1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Nucleotide-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03715</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853753.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6T1"/>
    <dbReference type="GeneID" id="33315348"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03715"/>
    <dbReference type="OrthoDB" id="323844at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021799">
        <property type="entry name" value="PIN-like_prokaryotic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39550">
        <property type="entry name" value="SLL0658 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39550:SF1">
        <property type="entry name" value="SLL0658 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11848">
        <property type="entry name" value="DUF3368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="158" mass="17174" checksum="ADA5E3D4BE64152D" modified="2017-09-27" version="1">MRVVFNSSPVIALAKLGYLKHAVRLFGEVFVPEAVVEDINAKEDEVSSVLSKLLKSGEVKMASPSREALPGYSGLHHGELEAIALAKELGAFVILDDLKARKAARLEGLSVIGTVAVLRLLKDAGLLKETDDELFNALRSVGFRIRPELFYKIMGGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6U7</accession>
    <name>A0A218P6U7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Replication factor A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_03625</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853739.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6U7"/>
    <dbReference type="GeneID" id="33315330"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03625"/>
    <dbReference type="OrthoDB" id="6262at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010212">
        <property type="term" value="P:response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04491">
        <property type="entry name" value="SoSSB_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051231">
        <property type="entry name" value="SOSS-B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006255">
        <property type="entry name" value="PRK08402.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356">
        <property type="entry name" value="OB FOLD NUCLEIC ACID BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356:SF10">
        <property type="entry name" value="REPLICATION FACTOR-A PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08646">
        <property type="entry name" value="Rep_fac-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OB" evidence="2">
        <location>
            <begin position="73"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Replication factor A C-terminal" evidence="3">
        <location>
            <begin position="193"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="357" mass="40493" checksum="4600080B61A7EBC9" modified="2017-09-27" version="1">MGVLTKEQVIEMIKRQKGLSEDEIEERIAEIAEREGISEHAAAVMLAEELGVNLEGKEELLHIADLVPGMTGVNVVARIMRKYPPREYQRRDGSTGRVANLIIYDGTGRTRLVLWDNLVGKYYEELKPGDVIKIIDPTVREGRNGIELHANFRTRIIPNPEDPRVEEIPPLEEIRSYNYQRRKIGELMGGEKFVEVRGTIARLYRVTAYDACPQCRRKVDYDPATETWICPEHGEVKPVTMVIIDFGLDDSTGYIRTTLFGDDAADLLGREPEEIAEKLKELVEGGLTLREAGRKLAEEEYYYLLGKEIIVRGNVVDDKFLGLILKAFGWDEVDPKREIARVRAELKKAIAELEGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Y4</accession>
    <name>A0A218P6Y4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03840</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853778.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Y4"/>
    <dbReference type="GeneID" id="33315373"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03840"/>
    <dbReference type="OrthoDB" id="102182at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="73" mass="8275" checksum="B74BA75E0866EE52" modified="2017-09-27" version="1">MGETLEVMKRSYQRFLAVGLGLMLIAFLLMIWQPLGREPSLVLAVIVFLVAFLPLEFARRIARKMALVALRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P726</accession>
    <name>A0A218P726_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Asparagine synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_04010</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853808.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P726"/>
    <dbReference type="GeneID" id="33315406"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04010"/>
    <dbReference type="OrthoDB" id="131570at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006421">
        <property type="term" value="P:asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005054">
        <property type="entry name" value="PRK06462.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF48">
        <property type="entry name" value="ASPARAGINYL-TRNA SYNTHETASE-RELATED PROTEIN (N-TRUNCATION)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="6">
        <location>
            <begin position="91"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="300" mass="34459" checksum="2933A4BE0E5CECD8" modified="2017-09-27" version="1">MNALQIVSRKIEPVLNIQTKVIDYMTRYMVSRGFKWMLPVMLSSITDPLWPDPAAEDALKPPEVEVYGSRLRLTHSMILHKQMAIAMGIDRLFILSPNVRLEGRDADDGRHAYEFTQLDFEIAYASMDDIMSLIEGLISGLFKEARSWGLEREIPKVRPPFRRFTLEEIKEEFGSEDEASRLMDEPFWITDIEREFYDREDPERPGHFRNYDLYLPEGYGEVSSGGEREWEYEKILARIRSSGLSEESFRPYLEVAKAGLLRPSAGAGIGVERLVRYMVGAKHIAEVQPFPRVPGVPAVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P773</accession>
    <name>A0A218P773_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_04430</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P773"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04430"/>
    <dbReference type="OrthoDB" id="24854at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050072">
        <property type="entry name" value="Peptidase_M20A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006326">
        <property type="entry name" value="PRK08554.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808">
        <property type="entry name" value="ACETYLORNITHINE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808:SF32">
        <property type="entry name" value="ARGE_DAPE-RELATED DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00759">
        <property type="entry name" value="ARGE_DAPE_CPG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="439" mass="48946" checksum="A659C558B9F09BAA" modified="2017-09-27" version="1">MEVLELLSELVSFETVNDPARGIKPSKDCPRFIMDRLSEWGIESRLLERDGYYAVYGEIGEGKPRLLFMAHFDVVPVNPAEWETDPFRLTVEGNRAYGRGSADDKGNVASIMLALRELSRERLNGKVLFAFTGDEEIGGRMAMHIAEKLSKEGKLPEYMINADGIGMVPIIRRRKGFGVMLRIPSERVRVKGRLREKTFRISTPVVETRHAAYFLPGVDTHPMIAASHFLRSRNALAVSLEGSFLKGNVVPGEVRLKYIEPGEGGDVEVDLGLTRLLKAIVPLVRAPIKTERYSDYGVSITPNLYTLEGGEHVLRIDVRAMSRSKEDIERAMREVVEFNLPGAELTVMTNEKAGYLFTQPGERIVRVMLEVLEGEGERAEPIEGPGAADSRFFTPYGVRAIDFGPRGGNIHGPNEYVEVDSLEKMPRIYKGVALRLLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P782</accession>
    <name>A0A218P782_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_04530</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853895.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P782"/>
    <dbReference type="GeneID" id="33315510"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04530"/>
    <dbReference type="OrthoDB" id="90770at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="387" mass="45502" checksum="DB35451438F83AC5" modified="2017-09-27" version="1">MKEVVLLTGPPLNGRDEYITEALKLVENESYAYYHVFDYIREVGKERGVKITRKNVLDFAINHQDLMNEIRDEAFERIRREIDGSDKVYHLVSTPSLFRWGSGSVIGFTASNLKLLKPDRVIIVLDDVLSVRRRIINDPEWFERFGENPENIKLTTLVMWREDAINHVKTLVHELKKEGLEVRYVLQFGIRHPHAVFLDLLFHENEKPLVYLSYPMTGHEEEYYHRVRGFYNKLSEHFTVLDPGALDDWWVVAEYDAQVNADPSVKRIRIKHLLDGEEVEELDRADIEQATDILRRQLVERDFNLVDVSKAIAVYHYAEGVSAGVISEMAEAYRTLAAIYLYYPFKRRPSPFMEFYGMQNPSRRTMFKDEDEMIKAMVEEKEYWAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7B4</accession>
    <name>A0A218P7B4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_04710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853929.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7B4"/>
    <dbReference type="GeneID" id="33315546"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04710"/>
    <dbReference type="OrthoDB" id="85879at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014509">
        <property type="entry name" value="YjdF-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09997">
        <property type="entry name" value="DUF2238"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="200" mass="22866" checksum="43251ED0FAA95613" modified="2017-09-27" version="1">MSRWRFVLNVCRFVVFLGLISGLMYMRVDHIYRSAVALVGLLILEFFQRKVAPPKRLQPFLKPLYNDKTMAILGIFIAVHVSLVNVPFTTIDLFHKEWTNADIISHFLGGLTVWVIVAEVLNEISKTCGFSERQIIFYSFVIMLVLGVGWEITERLSESKISYIRESLGNKARDLLMDTLGALLGVYMVKAGEFPFKLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7G9</accession>
    <name>A0A218P7G9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA-directed RNA polymerase subunit Rpo1N</fullName>
            <ecNumber evidence="13">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">DNA-directed RNA polymerase subunit A'</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">rpo1N</name>
        <name evidence="13" type="synonym">rpoA1</name>
        <name evidence="17" type="ORF">A3L08_05040</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Forms the clamp head domain.</text>
    </comment>
    <comment type="function">
        <text evidence="14">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 13 14">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds at least 2 Zn(2+) per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 13 14">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="13"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853979.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7G9"/>
    <dbReference type="GeneID" id="33315612"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05040"/>
    <dbReference type="OrthoDB" id="371812at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02582">
        <property type="entry name" value="RNAP_archeal_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.120:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00863">
        <property type="entry name" value="RNApol_arch_Rpo1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012758">
        <property type="entry name" value="RPO1N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006336">
        <property type="entry name" value="PRK08566.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02390">
        <property type="entry name" value="RNA_pol_rpoA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF32">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 13 14" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 13 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="16">
        <location>
            <begin position="211"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="680"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="461"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="463"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="465"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00053389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ASJ06724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="905" mass="102684" checksum="11A032751C50136E" modified="2017-09-27" version="1">MQSMKKVIGSIEFGILSPQEIRKMSAAEITVPDTYDDDGYPIEGGLMDKRLGVVDPGLRCETCGARAGDCPGHFGHVELARPIIHVGFAKTIHRVLESTCRECGRIKLKDEEIEEYMHKFDVVGDRKKAKDRLIKEIHKKAKERMVCPHCGAPQFPIKFERPTIYWELRKDEEGNEYKHRMMPSEVRDRLEKIPDKDLPLLGLHPEKARPEWMVLTVLPVPPVTMRPSITLESGIRAEDDLTHKLVDIIRINNRLKSNIEAGAPQLIIEDLWDLLQYHVTTYINNETSGIPPAKHKSGRPLKTLSQRLKGKEGRFRGNLSGKRVNFSARTVISPDPMISINEVGVPLAVAMELTVPEKVTEFNFEKLKQRVLNGPEKYPGANYVIDPEGRRIRLMENNLEIIAEKLDIGWTVERHLEDGDIVLFNRQPSLHRMSIMAHRVRVMPYRTFRLNLAVCPPYNADFDGDEMNLHVPQTEEAQAEAKILMEVQNHIISPRYGGPLIAGIQDHISGGYLLTREGAYFTRSEVEQMLMFAGVDVKELPEPDKVENGVELWSGKTIFSLLLPDDLTIWYRNKLCDEPERCEAIEKLIEEKLIPDPEEVRKIAYDGFVYIQNGKLLSGAIDKKAYGREDGKLLDIIVREYGVERARQFLDQVTKLAIWAITHKGFTTAIDDEDLPQEALDRIHEIIREAEDRVNRLIEAYKNGELEPLPGKTLEETLESNIMAVLAEARDNAGKVAEKYLGMTNHAVIMAKTGARGKMLNITQMAAMLGQQSIRGKRLYRGYRDRVLTHFKPGDLGAKARGFVTNSYKSGLTPQEYFFHAMGGREGLVDTAVRTAQSGYMQRRLINALQDLKVDYDGTVRDPMGIIVQFKYGEDGIDPMKSWQGKTIDVDRVIVRTLVKMRGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7I0</accession>
    <name>A0A218P7I0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Proteasome assembly chaperone family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853995.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7I0"/>
    <dbReference type="GeneID" id="33315634"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05150"/>
    <dbReference type="OrthoDB" id="31247at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004426">
        <property type="entry name" value="MJ1210-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00162">
        <property type="entry name" value="proteasome assembly chaperone family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610:SF7">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159659">
        <property type="entry name" value="Cgl1923-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="218"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="267" mass="29922" checksum="4AD7447A0DC211A7" modified="2017-09-27" version="1">MKETTIYVLERPELRDPVFIEGLPGIGLVGKLAAEHLIQELNAVKFAELYSPHFMHQVLIKKGSVVELMKNEFYYWKNPDENGRDVIIITGDQQVAPTDSPGHYEVVGKMLDFVSKFGVREIITMGGYQVPELQGEPRVLAAVTHEDLVGRYQEKLKDCQVEVIWREDEGGAIVGAAGLLLGMGKLRSMYGISLLGESLGYIVDAKSAKSVLLAVTKVLGLEIDMTALEERARETEEILRKVQEMQRAMLEQQMPPSTQEEEDRGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7I5</accession>
    <name>A0A218P7I5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cobalamin biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_05155</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853996.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7I5"/>
    <dbReference type="GeneID" id="33315635"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05155"/>
    <dbReference type="OrthoDB" id="71235at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000041">
        <property type="term" value="P:transition metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1760.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002751">
        <property type="entry name" value="CbiM/NikMN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34229">
        <property type="entry name" value="METAL TRANSPORT PROTEIN HI_1621-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34229:SF1">
        <property type="entry name" value="METAL TRANSPORT PROTEIN HI_1621-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01891">
        <property type="entry name" value="CbiM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="204" mass="21154" checksum="85B504DE5B4189B6" modified="2017-09-27" version="1">MHIPDGLLSTPVIAVTYIITALAVGYSARKLKDFPEEKIPLLGLFAAGIFAAQMVNFPIIGGVSGHLLGATLVAILLGPYAAVMVMTAVLLIQTLLFGDGGITAIGANILNMGLIGAFIGYALYTKLKNINETLAMGLSAWLSVVLGAALASVEIGLSHSLPFLRVLTLMVGYHSIIGIGEAVLTVLIVHAVRVKLPSIEGVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7J6</accession>
    <name>A0A218P7J6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Universal stress protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_05240</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854013.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7J6"/>
    <dbReference type="GeneID" id="33315652"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05240"/>
    <dbReference type="OrthoDB" id="107030at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="157" mass="18285" checksum="88F22E09D9467753" modified="2017-09-27" version="1">MFRKVLLPTDFSEGYCRAVLQFERMNEMRVGEVILLHVVDEGTLEELMDGYSLFYGDEEKELKDIEERLKEVAMEKLLERMDEIKRAFRAEKVTPMVRFGVPWEEIVKVAEEEDVSLIILPSHGKLGFSHESLGSTTMRVLKKTKRPVLLIKTHEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7N4</accession>
    <name>A0A218P7N4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MATE family efflux transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_05420</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854048.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7N4"/>
    <dbReference type="GeneID" id="33315688"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05420"/>
    <dbReference type="OrthoDB" id="214119at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13142">
        <property type="entry name" value="MATE_like_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048279">
        <property type="entry name" value="MdtK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052031">
        <property type="entry name" value="Membrane_Transporter-Flippase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43549:SF2">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN NORM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43549">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN YPNP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="199"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="247"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="305"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="342"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="379"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="412"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="438"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="469" mass="51186" checksum="78F913AEFB373601" modified="2017-09-27" version="1">MRSEKVEAMREQILNGPIVKTLILLAYPLIINQLVQVLYNLTDTFWLGKLGRTELSAPGTAWPLVWFFMSIGMGFATAGFAFVSQYVGAREYDKANRAAGALYSLMLIFATAVGITGVLLAPQLLHLMNVSDTIYPYALSYTRVIFGGIPFSFTLFAFNFLLRAVGDTKTPVKINIATVILNIILDPLFIFGWGPFPELGVVGAAIATMLSNTAGSIVGGYLLFTGKVGIHITPETLKPDFNFYSRIFRVGLPASIGSSTTALGFVILTRVIFTIGKLYGQAHGIPNFEDVAFATYSITNRLTNFMFAFSDGISMAMGTMVGQAIGAKLYDRAKTIAEKTMVINFTILGVGTLLFIFFRVPIFRFFINDPAVIAESAKVVKYFSASLPFFGIFAAVNNVFQSAGQTKKSMVLGMVRLWGIRLPLSYGLGVLMRDTAGMWLGMGLSNVIGAVVALAWFLRGSWMKAIIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7P0</accession>
    <name>A0A218P7P0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Small ribosomal subunit protein eS8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rps8e</name>
        <name evidence="7" type="ORF">A3L08_05465</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2 6">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic ribosomal protein eS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854056.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7P0"/>
    <dbReference type="GeneID" id="33315697"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05465"/>
    <dbReference type="OrthoDB" id="372305at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11382">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.310:FF:000002">
        <property type="entry name" value="30S ribosomal protein S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00029">
        <property type="entry name" value="Ribosomal_eS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001047">
        <property type="entry name" value="Ribosomal_eS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018283">
        <property type="entry name" value="Ribosomal_eS8_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020919">
        <property type="entry name" value="Ribosomal_protein_eS8_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022309">
        <property type="entry name" value="Ribosomal_Se8/biogenesis_NSA2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00307">
        <property type="entry name" value="eS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10394">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01201">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01193">
        <property type="entry name" value="RIBOSOMAL_S8E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6 7" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="130" mass="14474" checksum="3DB4318D16F345A9" modified="2017-09-27" version="1">MAIWQGRSLKKPSGGRIILARKKRKYELGREPAFTKVGEDREKKKIIRTYGGNRKVRLVEALYANVFDGGKGKKVKILNVVENPANRQYVRRNIITKGAIIETEAGKAIVTSRPGQDGVVNAVLIKEESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Q3</accession>
    <name>A0A218P7Q3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Probable molybdenum cofactor guanylyltransferase</fullName>
            <shortName evidence="8">MoCo guanylyltransferase</shortName>
            <ecNumber evidence="8">2.7.7.77</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">GTP:molybdopterin guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Mo-MPT guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Molybdopterin guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Molybdopterin-guanine dinucleotide synthase</fullName>
            <shortName evidence="8">MGD synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">mobA</name>
        <name evidence="10" type="ORF">A3L08_05335</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Transfers a GMP moiety from GTP to Mo-molybdopterin (Mo-MPT) cofactor (Moco or molybdenum cofactor) to form Mo-molybdopterin guanine dinucleotide (Mo-MGD) cofactor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Mo-molybdopterin + GTP + H(+) = Mo-molybdopterin guanine dinucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:34243"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:71310"/>
            <dbReference type="EC" id="2.7.7.77"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The N-terminal domain determines nucleotide recognition and specific binding, while the C-terminal domain determines the specific binding to the target protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the MobA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.77" evidence="8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854031.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Q3"/>
    <dbReference type="GeneID" id="33315671"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05335"/>
    <dbReference type="OrthoDB" id="28434at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061603">
        <property type="term" value="F:molybdenum cofactor guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02503">
        <property type="entry name" value="MobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00316">
        <property type="entry name" value="MobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013482">
        <property type="entry name" value="Molybde_CF_guanTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001457">
        <property type="entry name" value="PRK00317.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19136">
        <property type="entry name" value="MOLYBDENUM COFACTOR GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19136:SF81">
        <property type="entry name" value="MOLYBDENUM COFACTOR GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MobA-like NTP transferase" evidence="9">
        <location>
            <begin position="3"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="6"/>
            <end position="8"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="195" mass="21662" checksum="B795313FA44478F3" modified="2017-09-27" version="1">MIAAVLAGGSGRRFGGDKLLFRIDGRPLALHTIERLKKADSITKVVIVASPENADKLKAFGHVVVDELLVGPIGGVYTALSLGDAFVVAGDMPLLVPEFVDFIVERFKTAKKPACVPRWGNGYLEPLHAAYSSEFREFLEEKIKAGHYAINRAIMESDACYIEIESLPEEWRESFFNVNTRKDLGRIRGTRERVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7R6</accession>
    <name>A0A218P7R6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">AP endonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854081.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7R6"/>
    <dbReference type="GeneID" id="33315725"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05605"/>
    <dbReference type="OrthoDB" id="372143at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050312">
        <property type="entry name" value="IolE/XylAMocC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12110">
        <property type="entry name" value="HYDROXYPYRUVATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12110:SF21">
        <property type="entry name" value="XYLOSE ISOMERASE-LIKE TIM BARREL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylose isomerase-like TIM barrel" evidence="1">
        <location>
            <begin position="51"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="268" mass="29652" checksum="061AE423D8A28F87" modified="2017-09-27" version="1">MEIGITVYPHFVTKDKTLASVLADIKIKNYDFVSIFPHTLGLIMNGVVIEKKLRNIETTLRGVGIDYIIRMPTSVNLRDHIYYTRHFRVARAIADVAIKLGAKVIVMQSGRTGRLDLEIEAIQQLADMVAPFGIKIALENTFSVKDTLYVVDNVDRKNVGFALDVAHAFLSAQGDADKLLEDVKLGTDKTIILMIHDNFGKLFPQVEPEDALAYGVGDLHLLPGEGSIPFGKVLRLFGDVPLLLKVKDPEKFAKVPTKTGLIELLTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7W3</accession>
    <name>A0A218P7W3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05750</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854109.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7W3"/>
    <dbReference type="GeneID" id="33315754"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05750"/>
    <dbReference type="OrthoDB" id="85824at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="261" mass="28554" checksum="D627BE14DA4C75C8" modified="2017-09-27" version="1">MNKKFIGAIVILVLVLLPISYIGYGYTKFDEQIAPKPNAVSYQFVVIKPPESTNYVIISARTFLNLTQSGNWSPPTGSKIYPVTIITGITGDLGTDMNLTFRSRYERFTIIMGSTEVKTCSTDPSMFPGNCAERAAAVTEITAMASNIFKDHFYYEGLSQGMSESEAKAYAFKETTKRYGKAYLAFFTKAQIGLGTIGNEKHLAVILLGPAEGSESNRIIIPKQGLVVIEGKTDDALRAEIALIEKAINFSWPENNSTATG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7X0</accession>
    <name>A0A218P7X0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATP pyrophosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854141.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7X0"/>
    <dbReference type="GeneID" id="33315786"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05910"/>
    <dbReference type="OrthoDB" id="372052at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01994">
        <property type="entry name" value="AANH_PF0828-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1490.10">
        <property type="entry name" value="putative n-type atp pyrophosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002761">
        <property type="entry name" value="Diphthami_syn_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00290">
        <property type="entry name" value="MJ0570_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01902">
        <property type="entry name" value="Diphthami_syn_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Diphthamide synthase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="213" mass="23922" checksum="9EE0787773C16586" modified="2017-09-27" version="1">MKGVAFFSGGKDGFYALYLAEKSGIKVPYLLALKTTIGLSPHWENLDALKTLAEAMGKELLTFDMSRGSDALAEFIASLGVDYLIAGDVLLEDHKKWVEWLAEKAGVEALEPLWGRNTLELAREMLREGFEWAVIAVDKKKLPKEALAYTFRSQEDLDNFLEAYPGVAPVGEFGEFHTVVLASPIFEGRFELEVKSVEESERYHWIRFGLVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7X5</accession>
    <name>A0A218P7X5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Probable cobyric acid synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cobQ</name>
        <name evidence="10" type="ORF">A3L08_05940</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the CobB/CobQ family. CobQ subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854147.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7X5"/>
    <dbReference type="GeneID" id="33315792"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05940"/>
    <dbReference type="OrthoDB" id="53136at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05389">
        <property type="entry name" value="CobQ_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01750">
        <property type="entry name" value="GATase1_CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00028">
        <property type="entry name" value="CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033949">
        <property type="entry name" value="CobQ_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047045">
        <property type="entry name" value="CobQ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004459">
        <property type="entry name" value="CobQ_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00313">
        <property type="entry name" value="cobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001989">
        <property type="entry name" value="PRK00784.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21343:SF1">
        <property type="entry name" value="COBYRIC ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21343">
        <property type="entry name" value="DETHIOBIOTIN SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51274">
        <property type="entry name" value="GATASE_COBBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="8">
        <location>
            <begin position="5"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="CobB/CobQ-like glutamine amidotransferase" evidence="9">
        <location>
            <begin position="250"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="325"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="483" mass="53568" checksum="ABDB9E03EEAE2AF0" modified="2017-09-27" version="1">MGKALMVQGTSSGAGKSLLVTALCRIFSNLGYDVVPFKSQNMSLNSAPSIEGGEISRAQYLQAIACRKRPSVKFNPILLKPEGKMRSQVVFMGKPIGSVSAKDYMLSRKEELFKKAMKVLDELKEGHDLVIIEGAGSPVEINLKDYDIANMRVAKHADAKVILVTDIDRGGSFASIVGTMELLSEEERELVMGFVFNKFRGDASLLRPGFDYLEKRYGKPTLGVVPYVEHRLPEEDSLAEFPKVKGELHIQIIKLPHISNFTDFEPLHWANGVDYVTKAEEITGDVIIIPGSKNTVEDLLWMRENGIEDAILQAHREGAFVVGICGGFQMLGREIIDEVESKRGNVKGIGLLPAKTVFTRKKRTNHLKANVLWGPAGGMGVEGYEIRMGRSLSERPFSVITSVNGAKTFEPEGAIGERAFGTYLHGIFHNFAFTERFLNFLRAEKGLEPLSIEGWSIEEEIERFARVVGENFDVERILGELSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7X8</accession>
    <name>A0A218P7X8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF3194 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0440 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854158.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7X8"/>
    <dbReference type="GeneID" id="33315805"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06005"/>
    <dbReference type="OrthoDB" id="85298at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.100">
        <property type="entry name" value="MTH677-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024502">
        <property type="entry name" value="DUF3194"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035954">
        <property type="entry name" value="MTH677-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11419">
        <property type="entry name" value="DUF3194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110783">
        <property type="entry name" value="Hypothetical protein MTH677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="103" mass="11733" checksum="813B2372772E88FB" modified="2017-09-27" version="1">MAEGKRVIHIGLPELGEEELIEIGELAQETIIKEVFDVLNRSDVKDIEVTTRINRDETLDLEIEVYLEVPIFVKADVDELIDKAVEKAYEVVERRLKGLAAKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P808</accession>
    <name>A0A218P808_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9 10">Prefoldin subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">GimC subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pfdB</name>
        <name evidence="12" type="ORF">A3L08_06010</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">Molecular chaperone capable of stabilizing a range of proteins. Seems to fulfill an ATP-independent, HSP70-like function in archaeal de novo protein folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Heterohexamer of two alpha and four beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854159.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P808"/>
    <dbReference type="GeneID" id="33315806"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06010"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23162">
        <property type="entry name" value="Prefoldin_beta_GimC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00307">
        <property type="entry name" value="PfdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012713">
        <property type="entry name" value="PfdB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02338">
        <property type="entry name" value="gimC_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20903:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20903">
        <property type="entry name" value="PREFOLDIN SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="15"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="NCBIfam" id="TIGR02338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="117" mass="13266" checksum="F5C8BFD4BFF346A7" modified="2017-09-27" version="1">MQNIPPQVQAMLGQLESYQQQLQLVVQQKQKVQLELTEAKKALEEIEKVDDGTTIYKTVGTLIVKTEKAKAVEELKEKVETLEVRLNALERQEKKLNEKLKELTAQIQSALRPPTAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P810</accession>
    <name>A0A218P810_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">3-oxoacyl-ACP reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_06105</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854175.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P810"/>
    <dbReference type="GeneID" id="33315824"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06105"/>
    <dbReference type="OrthoDB" id="24596at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF133">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="4">
        <location>
            <begin position="6"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="240" mass="25375" checksum="D4C41DF6A46A26ED" modified="2017-09-27" version="1">MELAGKVALVTGAGRGIGRAIAVALARKGANVAVNYAHSAEGAWKTVELCRSHGVDAIAVKADVSNREEVRKMVEEVVGHFGRIDILVNNAGILGKALRPMEVTDEDWDAVLGVNLKGAFIVTQEVLRYMKKGKIVNIASIAGKDGGTVGPHYAASKGGLIALTFNLARHLAPDILVNAVAPGPVDTELISPDIKEKLKKLSLTGEIAKPEEIAHAVIFLLENDHITGELIDVNGGRLMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P830</accession>
    <name>A0A218P830_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_06145</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854181.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P830"/>
    <dbReference type="GeneID" id="33315832"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06145"/>
    <dbReference type="OrthoDB" id="6762at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154">
        <property type="entry name" value="LEUCINE-RESPONSIVE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR AZLB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="7"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="153" mass="17167" checksum="D20AAD937120F9E2" modified="2017-09-27" version="1">MRNQGKLDGLDRMILHILQEDGRASYSEIARRLKVPESTVRLRVKKLVESGVIRKFAALINPFKAGYSIVAFIAVDVEPSRVKNAAEELSKLPEVDVLGIATGAHDILMQVTVRDLYELENFLIEKLGKVEGIRSTETSILTSVRKWGFARVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P843</accession>
    <name>A0A218P843_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MrpA C-terminal/MbhD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_06215</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854193.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P843"/>
    <dbReference type="GeneID" id="33315846"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06215"/>
    <dbReference type="OrthoDB" id="99605at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1200">
        <property type="entry name" value="NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025383">
        <property type="entry name" value="MrpA_C/MbhD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042106">
        <property type="entry name" value="Nuo/plastoQ_OxRdtase_6_NuoJ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006242">
        <property type="entry name" value="PRK08378.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13244">
        <property type="entry name" value="MbhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="MrpA C-terminal/MbhD" evidence="7">
        <location>
            <begin position="13"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="94" mass="10427" checksum="1749EAC948002807" modified="2017-09-27" version="1">MNCITCVEYIIVALMILSAILAVEWRDLLAAVVGMAAVSLFASILFFMLQAPDVAMTEAAIGAALSGAVFIFAIKRTQRFETEEEEKPGWWVRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P855</accession>
    <name>A0A218P855_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HEAT repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06305</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854210.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P855"/>
    <dbReference type="GeneID" id="33315864"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06305"/>
    <dbReference type="OrthoDB" id="86199at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="189" mass="21035" checksum="2482197A52134466" modified="2017-09-27" version="1">MPFLSFGSKKGKVKKMFESDQLQLVVEEATKDKKTREALFELLQDDNPGVVGDALLTMVTLMDTNPDAIRPHLNGDAIKKLLSLVESRNPYVRENAMILLYSLAKSLPDLAAKYRGLIIEEAERALESGDKNQKGFILILIGELGLKELEPRVRGLVGVEDKVILPFEGRKWVKLGDIASETLEKLSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P887</accession>
    <name>A0A218P887_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06465</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854240.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P887"/>
    <dbReference type="GeneID" id="33315896"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06465"/>
    <dbReference type="OrthoDB" id="90469at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="71" mass="8570" checksum="506D2E944B697ED9" modified="2017-09-27" version="1">MGEVVIHVVVPDGYEETFKKEVEEMAKYLRNKEKLRKNMEKLFGVLKSDQTWKEMKREMYEGRLARHLDSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P896</accession>
    <name>A0A218P896_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Hydrogenase 3 large subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">hycE</name>
        <name evidence="11" type="ORF">A3L08_06525</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="544" mass="61516" checksum="397BB91B8E5E1EB0" modified="2017-09-27" version="1">MIKEFEARFGPILEKKTLGEKKVLYTIMARPEDFPDMVRFLLAGENVRLFTMVGTDERTVEDAFSITYWFADTSKGEILGLRLYVPEDRPVFPSVAQFHKGALWFEREVQDLLGVEAEGLPDRRRLVLPDDWPEGVYPLREDFKYSESPPGEKKYPYVRPPEGSSVHAIGPYHIALDEPAHFRLFVKGEEIVGVDYRGFYSHRGIEKLARGRLNYNQICFIAERICGICGFSHSTVYAQAIEEAAGIEVPERAEYIRTLLLELERLHSHLLWIGVAAHLVGFDTAFMDVWRIRERVMWLAERLTGNRKTYGLVVVGGVRRDLLDYRRELVERELAVLKKEFDNVVEFLLSSRGFIKRCEGVGVLPKEKARAWDVCGPVARASGVDYDVRRDFPYAAYGDLSFEVPLEKEGDVLARAKVRIEEVKQSISLIEQVLDAMPGGSILADFGDIPEGAEGISAVEAPRGENVHYVLTGDRNTLYRWRVKAATYNNLQAVPDMLVGYTIADAPLIIASIDPCYSCTERVQVVDIESGKSRVVRLGVGICL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8A8</accession>
    <name>A0A218P8A8_9EURY</name>
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        <submittedName>
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        </submittedName>
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                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">P-4</strain>
        </source>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ASJ07023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="175" mass="19043" checksum="D9224E1919ED9C18" modified="2017-09-27" version="1">MMVNWKSLLTWAGVGSFVGFAIAVSLYSSSGENEKAVYLIYAGLVAGILLSLKYRLELRASASAFPLGFLATSLLAALWMVTNVDPARIYAFIAVVMAVLMTIGPENYLDMFLAPLSYFGGFAVAMLTFKGYEPLQGTEGAVMSLFVVGVMGAILVFFALFARWAFEMARNISRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8C7</accession>
    <name>A0A218P8C7_9EURY</name>
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        <submittedName>
            <fullName evidence="7">Ribose ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_06835</name>
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    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
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            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
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                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
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        </subcellularLocation>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ABC TRANSPORTER, PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47089:SF1">
        <property type="entry name" value="GUANOSINE ABC TRANSPORTER PERMEASE PROTEIN NUPP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
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        <location>
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        </location>
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        </location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="337"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="350" mass="37402" checksum="8EBA8FAD20B6C1E2" modified="2017-09-27" version="1">MKKSGSDLTKALNPRPFIESLIAILVGFIIGAIVLVAFGYDPLAAYYWLFTGALGSARGIASTLKYATPIMLTALTFAIGTRTGIFNIGAESSFYFGAIAAVIFTNIWANMWFGLLMGMLFGALWALPAAALKVYRGVHEVISTIMLNWIGWFFVLWLIVGPYANPNDPNKTIRIPEAARLPEIGYGLSIAIIIAVIAALLTYFLLWHTTMGFGMRASGINQKAARYAGMNPKLAVMWSFIIGGLLSGLGGAMKIMGEGPGYAISQGGANIYGFGFDGIGVSLVGRNHPLGIILSALFFGMLRAGTAMMQARAQVPLEIIKVIQGIIVIAVAIPSLYDLVKKAVHRGAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8E7</accession>
    <name>A0A218P8E7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06880</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8E7"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06880"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="201" mass="22757" checksum="84B88ABD4DF0B19D" modified="2017-09-27" version="1">MLDEDNLEECIRKAALEALVDEATRNESDFVGKLFSPGLNYRIRECARPKAEVEFSLGRWLVAGGRADYLGFLEGLLCLIAWIDGRFREAQEIARITGVKLSGRIRGGMLVHGFGTGRWTSFAVKGDSLVATGDDDQREVPLNEVRDKVVDFLLGSLTWQMKELWERYSSARLEREFLHNTAPIRLLFKVIDHGGKLEVRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8I6</accession>
    <name>A0A218P8I6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">5'-nucleotidase SurE</fullName>
            <ecNumber evidence="4">3.1.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Nucleoside 5'-monophosphate phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">surE</name>
        <name evidence="6" type="ORF">A3L08_06805</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Nucleotidase that shows phosphatase activity on nucleoside 5'-monophosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18254"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.1.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="4">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the SurE nucleotidase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.5" evidence="4"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854300.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8I6"/>
    <dbReference type="GeneID" id="33315964"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06805"/>
    <dbReference type="OrthoDB" id="26873at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1210.10">
        <property type="entry name" value="Survival protein SurE-like phosphatase/nucleotidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00060">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030048">
        <property type="entry name" value="SurE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002828">
        <property type="entry name" value="SurE-like_Pase/nucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036523">
        <property type="entry name" value="SurE-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001491">
        <property type="entry name" value="PRK00346.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00087">
        <property type="entry name" value="surE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457">
        <property type="entry name" value="5'-NUCLEOTIDASE SURE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457:SF0">
        <property type="entry name" value="PHOSPHATASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G01070)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01975">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64167">
        <property type="entry name" value="SurE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Survival protein SurE-like phosphatase/nucleotidase" evidence="5">
        <location>
            <begin position="3"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="264" mass="28691" checksum="21B3BC52BE3A53D3" modified="2017-09-27" version="1">MRILLTNDDGIYSNGIRAAVKALSELGEVYVVAPLFQRSASGRAMTLHRPIRAKRIELPGAKAAYGIDGTPTDCVIFAIARFGDFDLAVSGINLGENLSTEITVSGTASAAIEAATHGIPSIAISLEVEWKKTLGEGEGIDFSVSAYFLKKIARAILENGLPEGVDMLNVNVPSDATEETEITITRLARKRYCPTVEERIDPRGHPYYWIVGKRTTEFEPGTDAYALKVERKVSVTPINIDMTAEVDFRMVMEILNLISSGPEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8J5</accession>
    <name>A0A218P8J5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06855</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854310.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8J5"/>
    <dbReference type="GeneID" id="33315974"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06855"/>
    <dbReference type="OrthoDB" id="85282at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="111" mass="12539" checksum="DFD518A15DE24F4D" modified="2017-09-27" version="1">MDREKKVEEIKSHSVYANEIYEMHSDSINEVLDKYEGLKESYLNDHSRARIVRIVFNEDNGLPLAIEFNRKDDSFKGFTIAIGKPHIKSNGDGKGEEEAEGNVPPQEDASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8L5</accession>
    <name>A0A218P8L5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cobalamin adenosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_07120</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854358.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8L5"/>
    <dbReference type="GeneID" id="33316027"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07120"/>
    <dbReference type="OrthoDB" id="4665at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008817">
        <property type="term" value="F:corrinoid adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1200.10">
        <property type="entry name" value="Cobalamin adenosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016030">
        <property type="entry name" value="CblAdoTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036451">
        <property type="entry name" value="CblAdoTrfase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029499">
        <property type="entry name" value="PduO-typ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00636">
        <property type="entry name" value="PduO_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12213">
        <property type="entry name" value="CORRINOID ADENOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12213:SF0">
        <property type="entry name" value="CORRINOID ADENOSYLTRANSFERASE MMAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01923">
        <property type="entry name" value="Cob_adeno_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89028">
        <property type="entry name" value="Cobalamin adenosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cobalamin adenosyltransferase-like" evidence="4">
        <location>
            <begin position="3"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="171" mass="19141" checksum="55D9351B1C89A88B" modified="2017-09-27" version="1">MSITTKTGDKGLTGLFTGDRVAKYSPIMEANGTLDELDSFLGEAKHYIPGEMAEILERVQVQLYDLMAELASKGKYAKVGEEDVKWLEGLIHKYEEEVQLRAFVLPGSTIASAKLDVCRAIARRAERKVAKLVLDYGFGNNALVYLNRLSDLLFIMARAIEKREGKLKEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8P7</accession>
    <name>A0A218P8P7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Inosine-5-monophosphate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_07445</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854416.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8P7"/>
    <dbReference type="GeneID" id="33316092"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07445"/>
    <dbReference type="OrthoDB" id="43333at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="11"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="76"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="136" mass="15070" checksum="5C6B50E6BF55C621" modified="2017-09-27" version="1">MEMKAPVKVYMNRKLIGVKPDDTVKRAGEVMVEFDIGSLVVVDEENSVVGFLTKGDLIRRVIVPGLPNTTPVKEVMTKELVTVPAETPLRDVLDIMSKKGIKHILIEERGKITGIFSITDLLEASRRRLETAIAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Q4</accession>
    <name>A0A218P8Q4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Restriction endonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854418.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Q4"/>
    <dbReference type="GeneID" id="33316094"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07455"/>
    <dbReference type="OrthoDB" id="330911at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1570.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029464">
        <property type="entry name" value="HSDR_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13588">
        <property type="entry name" value="HSDR_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Type I restriction enzyme R protein N-terminal" evidence="1">
        <location>
            <begin position="25"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="290" mass="33197" checksum="001E934958A3F12F" modified="2017-09-27" version="1">MEGLRKAVVRVRRKIASHRKLYEKNEEAVKQHLIGEIFQALGWDWNNPEEVRPEERTEDGRADYALILNGSVFAYVEAKNLSVNILKRDEPLRQLARYCFNSGVKYGILTNGAVWIAIKAFEEGSSLHDRILISANIEKEPLEKVVLKLSFLSKSRIVEMERLSSLLRALELSFSGLRREGYSDDMLLSYLTSREGSSTIPVEELSGNETPRAAYVYEGGWKLLPLPEQSIKGVLLSVLLYLEKRSQGRKAEEIRKAYEHIRRIQLEPKTALEVLKKLGEEEKLRISVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8R2</accession>
    <name>A0A218P8R2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Thermosome subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_07590</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone; binds unfolded polypeptides in vitro, and has a weak ATPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Forms a Heterooligomeric complex of two stacked eight-membered rings.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854442.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8R2"/>
    <dbReference type="GeneID" id="33316121"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07590"/>
    <dbReference type="OrthoDB" id="9362at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03343">
        <property type="entry name" value="cpn60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012714">
        <property type="entry name" value="Thermosome_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02339">
        <property type="entry name" value="thermosome_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="551" mass="59456" checksum="EA859DED2D822A33" modified="2017-09-27" version="1">MAQLSGQPVVILPEGTQRYVGRDAQRLNILAARIIAETVRTTLGPKGMDKMLVDSLGDVVVTNDGATILDRIDLQHPAAKMMVEVAKTQDKEAGDGTTTAVVIAGELLRKAEELLDQNIHPSIIVKGYTMAAEKAQEILEDIAIEVTPDDDDTLMKIAMTSITGKNAESHKELFAKLAVEAVKQVAEKKDGRYTVDLDNIKIEKKAGESVEESELVRGVVIDKERVHPRMPSRVEGAKIALINEALEVKKTETDAKINITSPDQLMSFLEQEEKMLKEMVDKIAETGANVLFVQKGIDDLAQHYLAKKGILAVRRVKKSDMEKLAKATGAKIVTNVKDLTPEDLGHADLVEERKIAGESMIFVEGCKNPKAVTILIRGGTEHVIDEVERALEDALKVVKDVMEDGYVLPAGGAPEIELSIRLDEYAKEVGGKEALAIENFAEALKIIPKTLAENAGLDTVEMLVKVISEHKNKGLGIGIDVFKGEPADMLANGIIEPLRVKKQAIKSASEAAIMILRIDDVIAAKVSKPEGGQGGGMPGGMGGMGGMDMGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8T0</accession>
    <name>A0A218P8T0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphonate ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_07620</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8T0"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07620"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001070">
        <property type="term" value="F:starch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002044">
        <property type="entry name" value="CBM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000801">
        <property type="entry name" value="Esterase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050583">
        <property type="entry name" value="Mycobacterial_A85_antigen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48098">
        <property type="entry name" value="ENTEROCHELIN ESTERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48098:SF6">
        <property type="entry name" value="FERRI-BACILLIBACTIN ESTERASE BESA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00686">
        <property type="entry name" value="CBM_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00756">
        <property type="entry name" value="Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01065">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51166">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM20" evidence="2">
        <location>
            <begin position="97"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="463" mass="51668" checksum="731DAB7A11BB610C" modified="2017-09-27" version="1">MQSVHLTRLLALMLAGMIVFSAGCLGNGGASVTTSPSQSEKTTSDPNGTAGVPHSTTPTTSAASTESTPTTTTSSTTAAETTSSETSTTTQTTSTPTETSRMVEVTFIVSVPDYTPENDPVYIAGDFNSWNPGDENYRLRKRDDGRWEITLEFPQGTSIEFKFTRGSWETVEKGRNGEEIANRRFTFKESGTYEFTVYHWRDYVEEVGVGEHTITGNVTTFKMLIPQLNRTRRIWVYLPPDYGTDGKRYPVLYMLDGQNLFDRATSFAGEWGVDEALERLYNESGFSLIVVGIDNGGDKRIDEYAPWVNEEYGRGGEGDAMVRFIVETLKPYIDLQYRTLPNETGIMGSSLGGLMAIYAGFSYPETFKYVGAMSSAFWFNPEIYDFVKNAPKGPEKIYIDWGTSEGSDPSEMIETNERMVEILKEKGYVEGENLMVVEDEGATHNEYYWGKRFPKAVLWLFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8U2</accession>
    <name>A0A218P8U2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_07575</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854439.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8U2"/>
    <dbReference type="GeneID" id="33316118"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07575"/>
    <dbReference type="OrthoDB" id="97152at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="169" mass="19565" checksum="E53A6ABDBA06EDDD" modified="2017-09-27" version="1">MVIVEKLLSPAYTVTVLNLKKFEPKDLLEGFKRRGFHIWKSLSGLDIELEIESFIASTYGKYVYVLKFKEETYLARGKEKVLRKEWKGPKTYLVRHVAGLKRLLLQELSLKAKLSLLLPQWILWSMVGVYALSWLKEDVLMSFLLTIFGFALNDIVKAVDYLILGYCNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8X9</accession>
    <name>A0A218P8X9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine hydroxymethyltransferase</fullName>
            <shortName evidence="9">SHMT</shortName>
            <shortName evidence="9">Serine methylase</shortName>
            <ecNumber evidence="9">2.1.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">glyA</name>
        <name evidence="12" type="ORF">A3L08_07920</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the reversible interconversion of serine and glycine with a modified folate serving as the one-carbon carrier. Also exhibits a pteridine-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SHMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.-" evidence="9"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854499.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8X9"/>
    <dbReference type="GeneID" id="33316189"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07920"/>
    <dbReference type="OrthoDB" id="5821at2157"/>
    <dbReference type="UniPathway" id="UPA00288">
        <property type="reaction ID" value="UER01023"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000101">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000114">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF35">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5 9" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8 9 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="11">
        <location>
            <begin position="10"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="122"/>
            <end position="124"/>
        </location>
        <ligand>
            <name>(6S)-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>(6S)-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
        </ligand>
    </feature>
    <feature type="site" description="Plays an important role in substrate specificity" evidence="9">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9 10">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ07251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="429" mass="48095" checksum="2251ED4EA69A57D0" modified="2017-09-27" version="1">MAEGYREYRDKVLDFIEEHENWRKHTINLIASENVTSPSVTRAVASGFMHKYAEGWPRQRYYQGCKYVDEVELIGVELFTKLFGSDFADLRPISGTNANQAVFFGLTQPGDKAIVLHTSHGGHISHMPFGAAGMRGLEVYTWPFDNEEFNIDVDKAEKLIREVEPKIVVFGGSLFPFPHPVKELAPVAKEVGAYVMFDAAHVLGLIAGKQFQDPLREGADIITASTHKTFPGPQGGVIIYKRFGETEEIAKLQWAIFPGVLSNHHLHHMAGKTITAAEMLEYGEKYAAQIVKNAKALAAALAEEGFKVIGEDKGYTESHQVIVDVSDLHEAAGGWAAPLLEEAGIILNKNLLPWDPLEKVEKPSGLRIGVQEMTRVGMMEDDMKEIAHFIKRVLLDKEDPKKVRRDVYGFRAEFQKVYYSFDYGLPMRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8Z0</accession>
    <name>A0A218P8Z0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA polymerase sliding clamp</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Proliferating cell nuclear antigen homolog</fullName>
            <shortName evidence="5">PCNA</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pcn</name>
        <name evidence="10" type="ORF">A3L08_07950</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication.</text>
    </comment>
    <comment type="function">
        <text evidence="7">Sliding clamp subunit. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotrimer. The subunits circularize to form a toroid; DNA passes through its center. Replication factor C (RFC) is required to load the toroid on the DNA. Interacts with TIP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the PCNA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854505.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8Z0"/>
    <dbReference type="GeneID" id="33316195"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07950"/>
    <dbReference type="OrthoDB" id="14749at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00577">
        <property type="entry name" value="PCNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.70.10.10:FF:000038">
        <property type="entry name" value="DNA polymerase sliding clamp 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00317">
        <property type="entry name" value="DNApol_clamp_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000730">
        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022649">
        <property type="entry name" value="Pr_cel_nuc_antig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022659">
        <property type="entry name" value="Pr_cel_nuc_antig_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022648">
        <property type="entry name" value="Pr_cel_nuc_antig_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00590">
        <property type="entry name" value="pcna"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002219">
        <property type="entry name" value="PRK01115.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002221">
        <property type="entry name" value="PRK01115.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF0">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02747">
        <property type="entry name" value="PCNA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00705">
        <property type="entry name" value="PCNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00339">
        <property type="entry name" value="PCNACYCLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01251">
        <property type="entry name" value="PCNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA N-terminal" evidence="8">
        <location>
            <begin position="4"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA C-terminal" evidence="9">
        <location>
            <begin position="126"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ07257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="249" mass="28087" checksum="72FDCDB01248B5D7" modified="2017-09-27" version="1">MPFEIVFDGAKDFADLIATASNLIDEAAFKITEEGISMRAMDPSRVVLIDLNLPESIFSKYEVEEEETVGVNMDHFKKILKRGKGKDTLILRKGDENFLEITFEGTAKRTFRLPLIEVEELELDLPELPFTAKVVVLGEVLKEAVKDASLVSDSMKFIARENEFIMKAEGETNEVEIKLTLEDEGLLDLEVEEETKSAYGISYLADMLKGIGKADEVIIKFGNEMPLQMEYPIRDEGKLIFLLAPRVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P905</accession>
    <name>A0A218P905_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_08065</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_198362108.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P905"/>
    <dbReference type="GeneID" id="60595940"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08065"/>
    <dbReference type="OrthoDB" id="1018at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01135">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="82" mass="9379" checksum="F1BDE240502B0194" modified="2017-09-27" version="1">MGSKVSRVCRTQEEAKKLYDRISRFYDLFAFPEGKYRERALELLSIKTRETVLEIGFGTGHALVRMAELVGKEGKVYLMSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P925</accession>
    <name>A0A218P925_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitroreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854526.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P925"/>
    <dbReference type="GeneID" id="33316220"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08075"/>
    <dbReference type="OrthoDB" id="10206at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02142">
        <property type="entry name" value="McbC_SagB-like_oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="entry name" value="NADH Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052544">
        <property type="entry name" value="Bacteriocin_Proc_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020051">
        <property type="entry name" value="SagB-type_dehydrogenase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03605">
        <property type="entry name" value="antibiot_sagB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43745">
        <property type="entry name" value="NITROREDUCTASE MJ1384-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43745:SF2">
        <property type="entry name" value="NITROREDUCTASE MJ1384-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="FMN-dependent nitroreductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="1">
        <location>
            <begin position="20"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="196" mass="21890" checksum="58A0FB3F4031BE60" modified="2017-09-27" version="1">MEVKLPEPRTQGEMSLEEAIYRRRSIRGYTTEPLTIEELSQLLWAAYGINAWGKRTSPSAGARYPFEVYVVVGNVEGLRPGLCHYDGKNHVLKLIREGDLRKELASACLGQRHVEEAPVNIVIAAHYERTTSRYGQRGIRYVHIDAGHMGQNIYLQATALGLGTVAVGAFRDDEVKRVIGVEGEPLYIFPVGRPVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P970</accession>
    <name>A0A218P970_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MBL fold metallo-hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08285</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854565.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P970"/>
    <dbReference type="GeneID" id="33316263"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08285"/>
    <dbReference type="OrthoDB" id="9180at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07709">
        <property type="entry name" value="flavodiiron_proteins_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045761">
        <property type="entry name" value="ODP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43041">
        <property type="entry name" value="HYDROLASE, METALLO-BETA-LACTAMASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43041:SF1">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19583">
        <property type="entry name" value="ODP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="33"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="257" mass="29212" checksum="DA99F9B88698CDE8" modified="2017-09-27" version="1">MIRNMNSYPLYDDGEHRVYWLGIEEAEDEKGILTNQYLVVDGNEAALIEPGGFFVFSRVLKNVSSIVPPTRIKYLMYSHQDPDVIAGLNLWFEYAPLAKFVISELWVRFLPHLAVLSAGRTIGIPDKGSSFNLGNSTIKAVPSHYLHSPGNFSFYDEKSGILFSADIGAAAFPEGEWYLFVEDFDEHAKYMEGFHKRYMSSTKALRAWARHVRKLNPKVIAPQHGAIFSDENVGKFLDWLESLEVGIDVFEEKFYGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P974</accession>
    <name>A0A218P974_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Galactose-1-phosphate uridylyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_08135</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the galactose-1-phosphate uridylyltransferase type 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854536.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P974"/>
    <dbReference type="GeneID" id="33316233"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08135"/>
    <dbReference type="OrthoDB" id="7650at2157"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008108">
        <property type="term" value="F:UDP-glucose:hexose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033499">
        <property type="term" value="P:galactose catabolic process via UDP-galactose, Leloir pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00608">
        <property type="entry name" value="GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000034">
        <property type="entry name" value="Galactose-1-phosphate uridylyltransferase galTa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001937">
        <property type="entry name" value="GalP_UDPtransf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005850">
        <property type="entry name" value="GalP_Utransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005849">
        <property type="entry name" value="GalP_Utransf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00209">
        <property type="entry name" value="galT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11943">
        <property type="entry name" value="GALACTOSE-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11943:SF1">
        <property type="entry name" value="GALACTOSE-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02744">
        <property type="entry name" value="GalP_UDP_tr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01087">
        <property type="entry name" value="GalP_UDP_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000808">
        <property type="entry name" value="GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Galactose-1-phosphate uridyl transferase N-terminal" evidence="9">
        <location>
            <begin position="2"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Galactose-1-phosphate uridyl transferase C-terminal" evidence="10">
        <location>
            <begin position="159"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-UMP-histidine intermediate" evidence="7">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ07290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="330" mass="38324" checksum="8DDC79277F6B7C27" modified="2017-09-27" version="1">MRELRYNPLTGQWVMVSAVRKKRPWRPKDYCPFCPGSEETGYGWEVLILPNRFPMLSFDAPRPENAGFYKKARAIGQCSVIVETPEHDLKDLDWLTHEQMAKVVKLWKNVTEELKKNPKVAYLAIFRNKGEEIGVSLIHPHGQLYALPFIPLKARLKLENSRNYYNRHGECLFCRILEEELKGERLIYENGSFAVFMPFFASWPFEIHIYPKRHVQWLTQLEENEVEDLAEAIRVATGTLNEVLGRDMPYSMMVLQAPFKGNYEFYHLHIEFYPILRDNGKIKYAAGIEMGTWEFTYDGVPEENAEKLREACKKVLGRLGAKGGCPEAVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P987</accession>
    <name>A0A218P987_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Biotin--[acetyl-CoA-carboxylase] ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_08410</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_198362110.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P987"/>
    <dbReference type="GeneID" id="33316288"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08410"/>
    <dbReference type="OrthoDB" id="46252at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin--[biotin carboxyl-carrier protein] ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16442">
        <property type="entry name" value="BPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004408">
        <property type="entry name" value="Biotin_CoA_COase_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003142">
        <property type="entry name" value="BPL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008988">
        <property type="entry name" value="Transcriptional_repressor_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00121">
        <property type="entry name" value="birA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006206">
        <property type="entry name" value="PRK08330.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835">
        <property type="entry name" value="BIOTIN PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835:SF5">
        <property type="entry name" value="BIOTIN--PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02237">
        <property type="entry name" value="BPL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50037">
        <property type="entry name" value="C-terminal domain of transcriptional repressors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="4">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="234" mass="26122" checksum="4C8E7F866E84AD4C" modified="2017-09-27" version="1">MDIKWKIIHLNEVDSTNEYAKRIAADSPEGTVVVAKRQTAGKGRKGRSWASPEGGLWLSVVFKPPRLDPRFVFVGALAVVDTLVDFGIASGIKWPNDVWAGERKIAGILTEGKGNEYTIMGIGINVNNPIPEELRESATSMFQLLGREVPLEKVLERLLFHLSGWYRVFLERPDLLMAKVREKTFILGRTVRVIRDGRELIGRAMDVLDDGSLLLDAAGQLKRILYGDVSLRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P989</accession>
    <name>A0A218P989_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_08390</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854585.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P989"/>
    <dbReference type="GeneID" id="33316284"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08390"/>
    <dbReference type="OrthoDB" id="97676at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11570">
        <property type="entry name" value="Protein of unknown function DUF257"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005489">
        <property type="entry name" value="DUF257"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03192">
        <property type="entry name" value="DUF257"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="219" mass="25086" checksum="A550A59F9576989F" modified="2017-09-27" version="1">MCDNRTITLEEFLSRLVRGEDVIIEYHSDEPVHILLLELLRETERKGGAVIIDELDQLHVFRVHLELSGLKTSPIDHSPVIKMGGIIDTGNVIGKIDMSKEPAVRKRYYEELLQKTGNPPFRVIVGFDKVLLRYENDPKEREKIFGHMVRPHIGSSERVAIYIVNRDLVNETTIKELREHATRVLKSEFNGEGFTLRVVKSIFPGEYGWTVSVPIKGNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9A4</accession>
    <name>A0A218P9A4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Antibiotic biosynthesis monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_08535</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854609.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9A4"/>
    <dbReference type="GeneID" id="33316312"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08535"/>
    <dbReference type="OrthoDB" id="88619at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="102" mass="12473" checksum="65C1B640CCC776E0" modified="2017-09-27" version="1">MAIMRLWHGRVPREKGDAYERFLIERAVPDYSSVDGLLKLYFTRRDEEKETHFLLVTIWDSWESIKKFAGENPELAKYYPEDDDFLIEKEKYVQHYEIFYEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9A9</accession>
    <name>A0A218P9A9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF1611 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_08610</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854621.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9A9"/>
    <dbReference type="GeneID" id="33316328"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08610"/>
    <dbReference type="OrthoDB" id="30617at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011669">
        <property type="entry name" value="DgcN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035086">
        <property type="entry name" value="DgcN-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035402">
        <property type="entry name" value="DgcN-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40690:SF1">
        <property type="entry name" value="DUF1611 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40690">
        <property type="entry name" value="GLL3100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07755">
        <property type="entry name" value="DUF1611"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17396">
        <property type="entry name" value="DUF1611_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026760">
        <property type="entry name" value="UCP026760"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-glutamate N-acetyltransferase-like N-terminal" evidence="2">
        <location>
            <begin position="39"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="D-glutamate N-acetyltransferase-like C-terminal" evidence="1">
        <location>
            <begin position="143"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="36734" checksum="FC4919AEE103D6AD" modified="2017-09-27" version="1">MEKALVLTEGRYLTTDGKTAHGLVRYSGKYKIAGLIDSTLAGRDAGEVLDGIKRGIPIYGTIEEALKENPDAKWLIIGVATPGGVLPPSYKRIVAKAIKAGLGVISGLHHFLSDDPYLKRLARQSGVEIIDVRKIFYNYRVPFTGKIEDVKATKVAVLGTDAAIGKRTTAIMLHEAFKALGLKSEFIAMGQTGWMQGFKYCIVMDSIINDFIAGAIEDVFYRAWAEERPDVIVTHGEGSLLHPAFPGGFELIGVARPDFIVLQHAPGRLTFDDFPQYRIPPLENYIRMILSGKPPIAITINTQNLTREEALEWAERIESETGIMTRVPFYQGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9D7</accession>
    <name>A0A218P9D7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_198362114.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9D7"/>
    <dbReference type="REBASE" id="210320">
        <property type="entry name" value="TpaP4McrBCP"/>
    </dbReference>
    <dbReference type="GeneID" id="33316338"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08660"/>
    <dbReference type="OrthoDB" id="9837at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052934">
        <property type="entry name" value="Methyl-DNA_Rec/Restrict_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37291">
        <property type="entry name" value="5-METHYLCYTOSINE-SPECIFIC RESTRICTION ENZYME B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37291:SF1">
        <property type="entry name" value="TYPE IV METHYL-DIRECTED RESTRICTION ENZYME ECOKMCRB SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="1">
        <location>
            <begin position="623"/>
            <end position="986"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="1120" mass="129004" checksum="A004C2575641E8AE" modified="2017-09-27" version="1">MPIPPDGITREHVLKAIDEINREGVPPKHQAQNTFLVVEGKKYPVKYVVLKAAKVAGVDISINEFTTIEAKNYLKRLGFNIGREEWELGIRILSKAGEGFIKRASEEGFNVSRNQLGYFSKKGHTIYYQLKLPPELNVSSKLVHFEWLARPEEEGAQFMVGLHLEFPREQRELNKKLADLLVEYVDLDELGKRVGAPVVREDKVVFTWFTANKTYESPADTEEEIVEWLVTSMLEFYKTFLPALREVLPMVLREDEVLEKKLKGIVLALKEKYQDDWNSIKEEINEKIEKVRKTILSNKEITKEDYREIDKTISEINRAFGKTVLWVWGSVNAGEEGALKFLNRQDFRELLAIGNAINEFEQVTSLRPRLESIMKKPHTKLSTISSWLCIVNPSIFIPANRQVFGYPVRQEIGVDIFFGSRSNVSTYLVILGALWKIKNEIGISDMLELAFYLSRADSEISSVQHWVEISGPPEEGYVGKILITPNKYQIKPGDVVYHYITDSGPEPYKKTFIGKSRVKEGFRKVPKEEALARLKEYVNWDEKFKKFAQERWGKARELYVTELEDFQEFKPPVPLEKVGGVFTPTPKYLVRGSSGLVRILEGLDSGDNMMKKIEEKIDSILSRKGQLILYGPPGTGKTWLASKYIRHKVPNGIHRLGKDSSLREDIKYYLLVMSTAKYDPSQIKEGLEEVFSGKLRHAFEDVEEGDIAFVYLTHPYKRITAIARCTGKTEEGAKFKIIKLVNGPTYEEMKREEPISDSPAVRTMLRGTLFPLSHEEAQWIASKIGFDTLKPLGVLDEGTKREFRAFEFVTFHPAFSYEDFVEGIKPETYEDPETGKKELLFKVEEGVFKRISRNAYNALLAWAGVEKEWTENTGLPSLSKDEIERIKRKIKNEAEGMPKFYLIIDEINRGDIPKIFGELITLLETDKRLFMDNETVTTLPYSKKRFGVPPNLHIIGTMNTSDRSIALMDVALRRRFGFIEMMPDYTVLNEHIIDNASEEVKPLAGIAVSALKALNKRIKREYDRDHQIGHSYYFRLNDHLNSREEFLKELKMIWFHEILPLLQEYFYDSPEKLERVLKSKNSENSFIRSSEDGIELKSEDEFTDDSFLEALKALIEAERE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9H4</accession>
    <name>A0A218P9H4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854681.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9H4"/>
    <dbReference type="GeneID" id="33316396"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08950"/>
    <dbReference type="OrthoDB" id="84937at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002749">
        <property type="entry name" value="DUF63"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40700:SF1">
        <property type="entry name" value="DUF63 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40700">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED, DUF63 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01889">
        <property type="entry name" value="DUF63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="268" mass="31393" checksum="74146B2E103BFDCD" modified="2017-09-27" version="1">MLESVWHEIWWFFHTYFWEPMFTRSGYNPVNTFVYAFLLGLGVIYSYRYIIKPLKIPVDERLFWAVTPMVVFGATVRALVDGGVLPKNPLILTPGVFFTAFFLIVPAIALDAKLKSYPKVTIAWGTILALWANYLLVTHAKNWEPYELTLVHTAVSWAVILAFYRWRPFDKLYLYAVLAHFYDMGSTVVAIQFYGYREVHWLENILVNHFGAYFYYPWITIILVVVYYGLKYLVPEEEERRFWYLAIYILGLGPAIRDPAQLVLQIPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9K9</accession>
    <name>A0A218P9K9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Nodulation protein NfeD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_09160</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854719.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9K9"/>
    <dbReference type="GeneID" id="33316438"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09160"/>
    <dbReference type="OrthoDB" id="28112at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000336">
        <property type="entry name" value="Membrane-bound serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052165">
        <property type="entry name" value="Membrane_assoc_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002810">
        <property type="entry name" value="NfeD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056738">
        <property type="entry name" value="NfeD1b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056739">
        <property type="entry name" value="NfeD_membrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33507">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YBBJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33507:SF4">
        <property type="entry name" value="NODULATION COMPETITIVENESS PROTEIN NFED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01957">
        <property type="entry name" value="NfeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25145">
        <property type="entry name" value="NfeD1b_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24961">
        <property type="entry name" value="NfeD_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141322">
        <property type="entry name" value="NfeD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="291"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="346"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="NfeD1b N-terminal" evidence="8">
        <location>
            <begin position="27"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="NfeD integral membrane" evidence="7">
        <location>
            <begin position="242"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="NfeD-like C-terminal" evidence="6">
        <location>
            <begin position="382"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="445" mass="47606" checksum="37A118CC6BF94A93" modified="2017-09-27" version="1">MRLNVALTILVLLMVFLPGVSAQGGTVYVAKVDGMITGYTVDQFDRYITMAEDANAEALIIELNTPGGRADAMQAIVSRIQSSKVPVIIYVHPSGGMAASAGTYIALGSHLIAMTPGTVIGACRPILGYGQNGSIVEAPPKITNFYVAYIRELAQMSGRNETLAEEFITEDRSVTPEEALKYGVIEVIATDVGDLLQKADGMETKLPVDGKGKVTLRLRDARLVYIEPSFRDTVVKYITDPTIAYLLINIGMIGLIFGFLTPGWHVPETIGAIMLVLGLVGLGYFGYESAGLVLLILAMIFFIAEALTPTFGLFTVAGVITFIVGGIMLFSGSGGEYLVTGETYSILRIAIIVTAVLLGLFFLFGVAAVVRAHKRKPEAGKEELVGTVGKVVEDLDPEGVVKLRGELWKARSSDGSRIPVGEKVRVVKVEGLTLIVKKVEGNEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9M2</accession>
    <name>A0A218P9M2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_09215</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9M2"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09215"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050547">
        <property type="entry name" value="DEAD_box_RNA_helicases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47963:SF8">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47963">
        <property type="entry name" value="DEAD-BOX ATP-DEPENDENT RNA HELICASE 47, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="195"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="342"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="353"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="375"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ07485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="396" mass="45071" checksum="84139F7D5232338B" modified="2017-09-27" version="1">MSGETDIIGQSQTGTGKTAAFALPIIEAIDPRERGVQAIILTPTRELALQVADEIKSLRGRKRVYVYAVYGGQPIGPQIRALERGTHVVVGTPGRVLDHIRRGTLDLSGVKFFILDEADRMLDMGFIDDIEAIFRETPEQKRVLMFSATMPPEIKRLARRYMGDYEVISVSSDELVPEMVDQEYLEVVPARKFTVLKKILDGNEDFYGIVFCATKRETHELSERLRSNGYRAEALNGDMSQAARERTFWRFKNKKTKVLVATDVAARGLDVQDISHIVNYSLPMTAEDYVHRIGRTGRMGKKGRAVTFIMPGEFRRLRYIAQQAGVEIRKSELSEEIPREYRERYERSQGHPGSRNYSRNSSRSRNYGSGSGKRNNGRSKKGGRKNYFDGYSDYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9P0</accession>
    <name>A0A218P9P0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ACT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09205</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854727.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9P0"/>
    <dbReference type="GeneID" id="33316447"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09205"/>
    <dbReference type="OrthoDB" id="86222at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="79" mass="9050" checksum="BA198043670D94DF" modified="2017-09-27" version="1">MQIVELDIKLPYEGRGKILSRLYSKVRGKIRDIHFLPPTSNGISEIRMEIVEDDAPKLLSELKKIIKNGRITFKVLSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9P9</accession>
    <name>A0A218P9P9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thil AANH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_09295</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854741.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9P9"/>
    <dbReference type="GeneID" id="33316465"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09295"/>
    <dbReference type="OrthoDB" id="372227at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:CCA tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002937">
        <property type="term" value="P:tRNA 4-thiouridine biosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020536">
        <property type="entry name" value="ThiI_AANH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050102">
        <property type="entry name" value="tRNA_sulfurtransferase_ThiI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006222">
        <property type="entry name" value="PRK08349.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43209">
        <property type="entry name" value="TRNA SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43209:SF1">
        <property type="entry name" value="TRNA SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02568">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thil AANH" evidence="3">
        <location>
            <begin position="2"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="196" mass="22079" checksum="18D3CC298A8E23BA" modified="2017-09-27" version="1">MKAVALLSSGIDSPVAIYLMLRKGLEITPVHFRQSRRKESKVMELYEILKRYGKLNEPVVIDAYEEQAPVFSKLAELGKAKWTCLFCKWTMVRKACRIGHEIGARAIVTGDSLGQVASQTLDNLVIISSASDLPILRPLIGMDKEEIVRIAREIGTFDVSIEPEEPCPFVPRHPVIRGSFGEFLRIRERLMKEGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9R2</accession>
    <name>A0A218P9R2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_09370</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854756.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9R2"/>
    <dbReference type="GeneID" id="33316480"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09370"/>
    <dbReference type="OrthoDB" id="31092at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010339">
        <property type="entry name" value="PRK13768.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF8">
        <property type="entry name" value="GPN-LOOP GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="247" mass="27768" checksum="339894DC7BEA579A" modified="2017-09-27" version="1">MILVFVGTAGSGKTTMSGAFGRYLEENSHSVGYVNLDTGVKNLPYPPDVDVREDITAWELMEEGYGPNGAIVESYDRLLAKLGDYASRIARLNEERDYVIIDTPGQMETFLFHEFGVRLMENLPEPLTVYLFGPEILRKPADFCFARFFSLMIDLRLGTTTVPALSKVDTVESIEDYRKFLDDIEYLTARLKLDPSTQGLLAYRMCSTLPELASPTRLLYLSAKTGEGFDELETLAYEHYCTCGDLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9T4</accession>
    <name>A0A218P9T4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09460</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854772.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9T4"/>
    <dbReference type="GeneID" id="33316498"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09460"/>
    <dbReference type="OrthoDB" id="96502at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="92" mass="10765" checksum="3848B29633B371D3" modified="2017-09-27" version="1">MDAREIVEILDEKGEVSLDTWKAVSVKKNKDGTADLLYRNLHVGTDDDPVFLWIYANIVEEDWDVRVLERITFKREDIAWLLRYVVKKGEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9W6</accession>
    <name>A0A218P9W6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Adenosylhomocysteinase</fullName>
            <ecNumber evidence="5">3.13.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">S-adenosyl-L-homocysteine hydrolase</fullName>
            <shortName evidence="5">AdoHcyase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ahcY</name>
        <name evidence="9" type="ORF">A3L08_09770</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine</text>
            <dbReference type="Rhea" id="RHEA:21708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="3.13.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5 6">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="5 6">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 7">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.13.2.1" evidence="5"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854829.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9W6"/>
    <dbReference type="GeneID" id="33316560"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09770"/>
    <dbReference type="OrthoDB" id="8479at2157"/>
    <dbReference type="UniPathway" id="UPA00314">
        <property type="reaction ID" value="UER00076"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004013">
        <property type="term" value="F:adenosylhomocysteinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071269">
        <property type="term" value="P:L-homocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033353">
        <property type="term" value="P:S-adenosylmethionine cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00401">
        <property type="entry name" value="SAHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000004">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1480">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00563">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00936">
        <property type="entry name" value="ahcY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004005">
        <property type="entry name" value="PRK05476.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420:SF0">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001109">
        <property type="entry name" value="Ad_hcy_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00738">
        <property type="entry name" value="ADOHCYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00739">
        <property type="entry name" value="ADOHCYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="2 5" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S-adenosyl-L-homocysteine hydrolase NAD binding" evidence="8">
        <location>
            <begin position="188"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <begin position="154"/>
            <end position="156"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="217"/>
            <end position="222"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="219"/>
            <end position="224"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <begin position="296"/>
            <end position="298"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001109-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ07586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="421" mass="46946" checksum="327F7F560B63DDD1" modified="2017-09-27" version="1">MDCTKDYCVKDLSLAPSGEKKIDWVSRFMPVLQHIRADFEKRKPFKGVRIATTLHLEMKTAFLLLTLKAGGAEVSAAASNPLSTQDDVVAALAKAGVKVYAIRGEDKEQYYEFMHKALDIEPNIIIDDGADMVSTVLKERPELIPEIWGASEETTTGVIRLRAMEKDGVLKFPIIAVNDSYTKYLFDNRYGTGQSTWDGIIRTTNLLVAGKNVVVVGYGWCGRGIAMRARGLGATVIVVEVDPIRALEARMDGFLVMDMKSAAKVGDIFVTSTGDINCIRKEHFELMKDGVIMANAGHFDVEISKPDLEALSVEINEVRPNIREYKMADGRRLYLLADGRLVNLAAADGHPAEIMDMSFALQAKAAEYILENHEKLEPKVYVLPREIDEMVARIKLASMGIKIEELTEEQRRYLESWEHGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Y1</accession>
    <name>A0A218P9Y1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transglutaminase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854817.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Y1"/>
    <dbReference type="GeneID" id="33316546"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09700"/>
    <dbReference type="OrthoDB" id="85899at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.620.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transglutaminase-like" evidence="1">
        <location>
            <begin position="296"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="535" mass="60902" checksum="EE16517E61C004D1" modified="2017-09-27" version="1">MGMKRILLVFPLLVLVMASGCLFKPPAEVRFSLDRTTVAPDDTIHVIVLINNTGKVGLTGANLVLGDSNFQILQEPKFPDVLPVGETAQLVWILKAPPTPGHYNLKLSLELTDELKRTWTGFYGQFLVTVSNKTPPRGELKLDVLGPETLRGGEVSNLSVTITNPLDVPIELTNIKLDLLEGMKVLSVSTMPETIHEGKTISLKYTVKAPYAYREGYISAVLRYRIGDMEKSVVKSVPLRVVWTPWNESEETLKEAYGLKYHWITDSYLVDGYWAEKYNSTPVFERSELRKKTLGVIGAAESEVQAAEAIYRWMMHTYSFGDTTSTLEPDRILLQDRISYAEGQILMTAMLRSIDIPARIVTLYNGTDCTRRPITEFYTVDGWYVVDIEHGFVGSLDEYLASPYFPRLYQMITGEGYRLVAQSPTELRGHEHVDVTGDFIADLEDRLLTVINGRLQPELRSKLMVVMNNLDENERLYALFLFSSAPSDDDLNRVIEEYSTKRIEQNVKTMYEFYRGMEWSDDFTRYWRIFAGEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Y3</accession>
    <name>A0A218P9Y3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Y3"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00480"/>
    <dbReference type="OrthoDB" id="9837at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052934">
        <property type="entry name" value="Methyl-DNA_Rec/Restrict_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37291">
        <property type="entry name" value="5-METHYLCYTOSINE-SPECIFIC RESTRICTION ENZYME B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37291:SF1">
        <property type="entry name" value="TYPE IV METHYL-DIRECTED RESTRICTION ENZYME ECOKMCRB SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="1">
        <location>
            <begin position="30"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="399" mass="46115" checksum="0044908480DAA7A2" modified="2017-09-27" version="1">MVIKLDEYFTSKGYLYPSYLIAQFYTALKTKGFVILSGLTGTGKTKIAQELAELLDDSGKNFLFLSVRPDWRDSKALLGYYNPLNGKYHKTKLLEFILEAKRDYEQNRGDAKPYFVLLDEMNLAHVEYYFADFLSVLESGRDEDGFTREPLKLHGVDKVEEEGIPKEIYLPPNLYIIGTVNMDETTYAFSPKVLDRAFVVEFHDVDLEGYPPETDEPTLEAIESLRRTILKDLKGPNGKFLSRSKDELNEAVREFKGKHLDYREILITLNKALEPYDMHFGYRVVDEIALFFKSTKESEEAGIVEFENDDEIFDLALLMKVLPKFHGNRKKLEKPLKEALKLCLAKDSGLKVNELRSADIVEILGDWENQKTNFRFKHTARKVLRMLRQLYEIGFASFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA02</accession>
    <name>A0A218PA02_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Effector of murein hydrolase LrgA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_01550</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854858.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA02"/>
    <dbReference type="GeneID" id="33314915"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01550"/>
    <dbReference type="OrthoDB" id="85741at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005538">
        <property type="entry name" value="LrgA/CidA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33931:SF2">
        <property type="entry name" value="HOLIN-LIKE PROTEIN CIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33931">
        <property type="entry name" value="HOLIN-LIKE PROTEIN CIDA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03788">
        <property type="entry name" value="LrgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="115" mass="12431" checksum="393F562CDC82221C" modified="2017-09-27" version="1">MKPYRGLAIIFGFYALGEFTSSALDLPIPGSVLGMLYLLAALLGGAVKLDWVENEAELFVRNMSIMFVPPGVGIITYIGLLKSQAVPVFGALIISFLITLLVTAKTVELLRGERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA25</accession>
    <name>A0A218PA25_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Methyltransferase-like protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_03525</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. PrmA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854881.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA25"/>
    <dbReference type="GeneID" id="33315310"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03525"/>
    <dbReference type="OrthoDB" id="31271at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051720">
        <property type="entry name" value="rRNA_MeTrfase/Polyamine_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23290">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23290:SF0">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase small" evidence="3">
        <location>
            <begin position="39"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00041374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="210" mass="23360" checksum="C71BD535979A127F" modified="2017-09-27" version="1">MKKKHLAMTLSKLKGFPKPKQELEQYRTPGNVAAELLWLAHSLGDIEGRVIADLGAGTGVLSIGAALLGAKKVYAVEIDGNALRIAKENARSLGVECVEFINADVSGFSERVDTVVMNPPFGSQVKHADRPFLLKAFEVSDVVYSIHLAKPEVRGFIEAFTRDNGFEITHRITLPFEIPAQFHFHKKRLERIMVDVYRFERVGNGKAETE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA40</accession>
    <name>A0A218PA40_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Class III signal peptide-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA40"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03330"/>
    <dbReference type="OrthoDB" id="85852at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="191" mass="20793" checksum="62BBF08C481904EA" modified="2017-09-27" version="1">MRIVRRGQVSLEFMLVFGIMLVLMLYSVNSITFQQGSTSAETLNMQILLEEKNLANTIAGTVAQVYAQGPGAKSTTYAKVTYLAEPDYLQKTFGSTSVTIKGSGNSVMVWVGSNSVTSGDNKNTVTTEVPYSFKEASLPFPNGLPSKSVRIVVEWNPDKAEGWSSKTVDGYLEIRININPVGDHEEGTDQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA48</accession>
    <name>A0A218PA48_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA48"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06670"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="70" mass="8093" checksum="4F597173B93AFD7B" modified="2017-09-27" version="1">MKSTLLEKRLEVVKKRKELLALEEARLVRLARQKKAAAAQLTKVKKEKFAVMMEEAKLLRALKQNTYPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA57</accession>
    <name>A0A218PA57_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Small ribosomal subunit protein uS5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rps5</name>
        <name evidence="12" type="ORF">A3L08_04150</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">With S4 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 8">Part of the 30S ribosomal subunit. Contacts protein S4.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">The N-terminal domain interacts with the head of the 30S subunit; the C-terminal domain interacts with the body and contacts protein S4. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 10">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA57"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04150"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000002">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000004">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01307_A">
        <property type="entry name" value="Ribosomal_uS5_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_uS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047866">
        <property type="entry name" value="Ribosomal_uS5_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_uS5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005711">
        <property type="entry name" value="Ribosomal_uS5_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_uS5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_uS5_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003125">
        <property type="entry name" value="PRK04044.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01020">
        <property type="entry name" value="uS5_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718:SF4">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="RIBOSOMAL S SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 8" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="S5 DRBM" evidence="11">
        <location>
            <begin position="54"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ07643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="229" mass="25527" checksum="980BF0D5FFD61F66" modified="2017-09-27" version="1">MAQRVLEEWEPRTKLGKLVKEGQITDIHEIFRKGYQIKEPEIVDVLLPEVNLRENQEVLDIALTVRMTDSGRRIRFRVLAAVGNRDGYVGLGIGHGKEVGIAIRKAIDYAKMNIIEIKRGCGSWECRCRRPHSIPFAVEGKEGSVKVKLMPGPRGLGLVIGDVGKKILSLAGVQDVWSQTLGETRTTVNFAKAVFNALYNTNSVAVKPEDIERYGIIVGREMSANFQVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA59</accession>
    <name>A0A218PA59_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">HAD family hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_02955</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854873.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA59"/>
    <dbReference type="GeneID" id="33315197"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02955"/>
    <dbReference type="OrthoDB" id="25155at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07530">
        <property type="entry name" value="HAD_Pase_UmpH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000053">
        <property type="entry name" value="TIGR01457 family HAD hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF46">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000915">
        <property type="entry name" value="PGP-type_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01139">
        <property type="entry name" value="C2.A:_Pyridoxal_Phosphate_Phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="272" mass="29928" checksum="A7A07D4C10581DF6" modified="2017-09-27" version="1">MSGGIGLIFDMDGVLYRGNKPVEGAGELINFLKEWGIPFIFLTNNSTRDPSMYREKLLSMGIDVSEERIVTSGLATRLYMEKHFEPGRIFVIGGDGLLKEMERLGWGVVGVEEAGNGGWKEVKYVVVGLDPYLTYEKLKYATLAIRNGARFIGTNPDTTYPAEEGLYPGAGAIIAALKASTDVEPLIIGKPNEPAYEVAREKLDGVEEIWMVGDRLDTDIAFAKRFGMKAIMVLTGVSGLEDIEKSGIRPDIVLPSVKELIEYLKVKLEEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA81</accession>
    <name>A0A218PA81_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA81"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09175"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="83" mass="9736" checksum="4A642ED55C3FDC3F" modified="2017-09-27" version="1">MMASAIIYGIVIILILINIVLLMLYYSAKSNPYYVVYDEETKSALKRRVSTLKEDLESELVDFDVEEWERTLEESIDEEVRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P4Y8</accession>
    <name>A0A218P4Y8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Histone</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_00085</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal histone HMF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853105.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P4Y8"/>
    <dbReference type="GeneID" id="33316808"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00085"/>
    <dbReference type="OrthoDB" id="7514at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22909">
        <property type="entry name" value="HFD_archaea_histone-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050947">
        <property type="entry name" value="Archaeal_histone_HMF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050004">
        <property type="entry name" value="HmfB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004823">
        <property type="entry name" value="TAF_TATA-bd_Histone-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF043032">
        <property type="entry name" value="archaea_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47828">
        <property type="entry name" value="ARCHAEAL HISTONE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47828:SF1">
        <property type="entry name" value="ARCHAEAL HISTONE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00803">
        <property type="entry name" value="TAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TATA box binding protein associated factor (TAF) histone-like fold" evidence="7">
        <location>
            <begin position="12"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ05842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="67" mass="7239" checksum="1CD0D4E1F35D365B" modified="2017-09-27" version="1">MAELPIAPVDRLIRKAGAARVSEDAAKLLAEHLEEKAMEIAKKAVDLAHHAGRKTVKAEDIKLAIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P522</accession>
    <name>A0A218P522_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF2178 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_00225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853132.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P522"/>
    <dbReference type="GeneID" id="33314649"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00225"/>
    <dbReference type="OrthoDB" id="96209at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019235">
        <property type="entry name" value="DUF2178_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09946">
        <property type="entry name" value="DUF2178"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ05869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="96" mass="11122" checksum="410CD9F241602F9A" modified="2017-09-27" version="1">MGSGLLFLFIAAVTGIYWFMFWRFMKETGQMKDERGRRINQIASEKILIIVQMMLLVGLLASEKFETLDASKILALIYVVAIFGHASLRYYYSRVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P538</accession>
    <name>A0A218P538_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable glycine cleavage system H protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gcvH</name>
        <name evidence="6" type="ORF">A3L08_00360</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="3">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the GcvH family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853158.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P538"/>
    <dbReference type="GeneID" id="33314676"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00360"/>
    <dbReference type="OrthoDB" id="9810at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06848">
        <property type="entry name" value="GCS_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00272">
        <property type="entry name" value="GcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002930">
        <property type="entry name" value="GCV_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033753">
        <property type="entry name" value="GCV_H/Fam206"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017453">
        <property type="entry name" value="GCV_H_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00527">
        <property type="entry name" value="gcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002270">
        <property type="entry name" value="PRK01202.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715:SF3">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01597">
        <property type="entry name" value="GCV_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="5">
        <location>
            <begin position="29"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-lipoyllysine" evidence="3 4">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR617453-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ05895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="134" mass="14993" checksum="6AAD55BEE431C515" modified="2017-09-27" version="1">MIEVGEYKVKEGLYYTKDHEWAQVLEDGTVLVGISDYAQKELGDLAYVELPEVGKEVSKGDVLCELESVKAVSEVYAPVSGEVAEVNEALEDSPELLNEDPYENWIAKLKPSNLDEELKELMDAEAYAEYLESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P583</accession>
    <name>A0A218P583_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">D-nopaline dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_00635</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853208.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P583"/>
    <dbReference type="GeneID" id="33314731"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00635"/>
    <dbReference type="OrthoDB" id="85124at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.440">
        <property type="entry name" value="2Fe-2S iron-sulphur cluster binding domain, sarcosine oxidase, alpha subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042204">
        <property type="entry name" value="2Fe-2S-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051691">
        <property type="entry name" value="Metab_Enz_Cyan_OpOx_G3PDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041117">
        <property type="entry name" value="SoxA_A3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42949">
        <property type="entry name" value="ANAEROBIC GLYCEROL-3-PHOSPHATE DEHYDROGENASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42949:SF3">
        <property type="entry name" value="ANAEROBIC GLYCEROL-3-PHOSPHATE DEHYDROGENASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13510">
        <property type="entry name" value="Fer2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17806">
        <property type="entry name" value="SO_alpha_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="2">
        <location>
            <begin position="110"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="SoxA A3" evidence="3">
        <location>
            <begin position="418"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ05946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="494" mass="54865" checksum="BB56C9C36059D6C4" modified="2017-09-27" version="1">MRPLDLTEKDPSKKITIYFEGKPYKAYEGEKLPVAMLANGIYWLTTSLQGRKRGAFTFGPLPVTVNGVKNVNGRKLKVRDGMRVERQNYGEFQEAVEVDGGKEPLREVVDVAVIGGGIAGIGAALEVQEHLTVALIEEKGWLGGLMFIESVPQGGFDEEPRKVVKKLTSEFNENVRVFTGTTALGIFDEGEYFLVPVVKGDQLIEIMAKRVVLATGAVENIVLFENNEFPGVFRLDFALEVINKWGVAPGKRVAVVGRKPERIIPELEKWGIEYVVVPNPKRAEGGERVERLIDMNGNVYEVDAVIVSDYRIPDINPVTQAGAKLKFRYGYYVPVTDGQNRIRDGIYVAGSAVSIKPHYANYLEGRLVGAYILREFGYDASPAVYREKLEEYEPESMPRFTLSLGDMNLEDVQICGCDVNLKKVDDVVKSGITDLQIIKRLTHLAMGFCQGRFCLFNGALLASQRTGTPMDKLDIPVARPPIKNVKMKVVARGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5C0</accession>
    <name>A0A218P5C0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Biotin synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_00855</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853245.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5C0"/>
    <dbReference type="GeneID" id="33314775"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00855"/>
    <dbReference type="OrthoDB" id="15118at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034422">
        <property type="entry name" value="HydE/PylB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43726">
        <property type="entry name" value="3-METHYLORNITHINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43726:SF1">
        <property type="entry name" value="BIOTIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01098">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="23"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ05983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="317" mass="36056" checksum="EF033DC1A9CD9B2C" modified="2017-09-27" version="1">MVEMIRVSYGTAVAMGLRRGKMLARPTTAYLMTYWPGHCRNNCAFCVQARESRADLEKLSRVTWPAFELEEVLDALPKGNFSRICLQTIDYPGMVEDVLDLLEAFAPMNLPVSVSITPVGKETLREFKRRGVDYVGVGLDAASERVYRGVKDAIYSWDDMWEFTRDIIDVFGPKSAFVHLIVGLGETDMEMIETIARAYGMDAEVSLFAFTPVKGTRLENARPPSLERYRRIQLARYLLEKGLSSPEDFEFDENGNLIGLGVPKEKLVELIPLEVFTTHGCPGCNRPYYNERPKREPYNFPVKPEKSYVERVLESIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5D8</accession>
    <name>A0A218P5D8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MFS transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_00840</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853242.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5D8"/>
    <dbReference type="GeneID" id="33314772"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00840"/>
    <dbReference type="OrthoDB" id="29061at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050189">
        <property type="entry name" value="MFS_Efflux_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43124:SF3">
        <property type="entry name" value="CHLORAMPHENICOL EFFLUX PUMP RV0191"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43124">
        <property type="entry name" value="PURINE EFFLUX PUMP PBUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="260"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="5"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ05980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="378" mass="40461" checksum="828C17C7A3EE061B" modified="2017-09-27" version="1">MRKRLLALLSLGWIFNYAHRMAIPPLIPMIKAELGINNAEAGLLMTALLLPYALVQVPAGYLGDRLGRKRLLVMSIIGYSLSSALIVFARQYWELLAVRALYGVFSGFYYAPATALISEVYRERKGSALGVFMIGPPVGTGIAPLIVVPIALSLQWRYAFAVLSAMSLLVGLALIFTVRGEVSKPQRASFSIPGNVFLLSAANFVVLAAFFGLLTFLVSFLVDSGVSIESASALFSLLSAIGIAGSLLGGGLYDRMGRGSIPAVFGLNAVLTFILAITASPWVVIPLGLTFYSVGAIVTAYTSEKATPENLGSVMGFVNMVGFFGATVGPYLLGLLIDRFGYEKAFLSVSAMYLLAWVLIKLEDLAEGRKEKSGISRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5F7</accession>
    <name>A0A218P5F7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SHOCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_01155</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853298.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5F7"/>
    <dbReference type="GeneID" id="33314835"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01155"/>
    <dbReference type="OrthoDB" id="101685at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018649">
        <property type="entry name" value="SHOCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039519">
        <property type="entry name" value="YokE-like_PH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14470">
        <property type="entry name" value="bPH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09851">
        <property type="entry name" value="SHOCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YokE-like PH" evidence="2">
        <location>
            <begin position="17"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="SHOCT" evidence="1">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="22836" checksum="542D047860D6591D" modified="2017-09-27" version="1">MGKEENPKLPKSVLRHLEPNEDVLFSIRKKISMEKPKWLLVTNRRIIYLDEKVLGRYDLKALPYQKLEQVTVKLGIMSSEFIIEGEENITLKLGWMNKEEARKAINAIKDALNAIAVEPVSIEVNKKLTSETWVLKKPKEFITRTMPAYQPAQPVVKEEKEDPMEQLKKLKELYDMGVISQEEYEEKRKRLLEQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5H0</accession>
    <name>A0A218P5H0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853295.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5H0"/>
    <dbReference type="GeneID" id="33314831"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01135"/>
    <dbReference type="OrthoDB" id="91238at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="127" mass="14339" checksum="2627C23B031B673C" modified="2017-09-27" version="1">MGLIKKLVVGFFLLLFIGAVGIFGMQYYYLNTYDKVPVEVKTETVSGDVFYLSHVLPAGKYYINASSEGTVEKIVILDEKDNVLTESETNSMIYGSSKPFQVRVEYKAPLNVDSYRVSVGIYRLEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5H3</accession>
    <name>A0A218P5H3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphomethylpyrimidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853284.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5H3"/>
    <dbReference type="GeneID" id="33314819"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01075"/>
    <dbReference type="OrthoDB" id="26806at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019293">
        <property type="entry name" value="ThiN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40730:SF4">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40730">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10120">
        <property type="entry name" value="ThiN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="28"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="300" mass="33031" checksum="A3607308F7B17B05" modified="2017-09-27" version="1">MRTPSVYIAEELMPYLRAQVSKKLYMEGMTQSQIAEYLGITQAMVSKYLSGKYKRPPEEVARKLDSIADEVVRVILFGGSRAEVVVITTRRLFDLFQSGFLCRFYSAYSGVSEELCRSMFSVQRDRGEVLEVLNLALAELAKLEGFPSLIPEVRSNFAYALPSPRGPEDVAAVPGRITTARGRIFALPPEFGASRFTAGVLVELGKLAPGIRSVLNIRYGDDVEEAMEMAGFRAARVKTGGLSEKEAVKSIAEVFESETYDAVVDEGGPGVEPVVYIFGEDPFEVVDKVKKLVESLEEVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5K1</accession>
    <name>A0A218P5K1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Nascent polypeptide-associated complex protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 7" type="primary">nac</name>
        <name evidence="7" type="ORF">A3L08_01285</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Contacts the emerging nascent chain on the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Interacts with the ribosome. Binds ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NAC-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853321.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5K1"/>
    <dbReference type="GeneID" id="33314862"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01285"/>
    <dbReference type="OrthoDB" id="53273at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14359">
        <property type="entry name" value="UBA_AeNAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="entry name" value="Nascent polypeptide-associated complex domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00814">
        <property type="entry name" value="NAC_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044034">
        <property type="entry name" value="NAC-like_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005231">
        <property type="entry name" value="NAC_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00264">
        <property type="entry name" value="archaeal-type nascent polypeptide-associated complex protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19026">
        <property type="entry name" value="UBA_HYPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51151">
        <property type="entry name" value="NAC_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NAC-A/B" evidence="6">
        <location>
            <begin position="3"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="NCBIfam" id="TIGR00264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="111" mass="12494" checksum="93ABD92FCC9DB312" modified="2017-09-27" version="1">MMGMNPRQMKKLMRQMGIKMEELEGVKEVIIRMENKEIVLKEPAITVITAQGEKSYQIVPGSEEVRAIINVSEEDIKLVMDQTGADYETARKALIEANGDLAEAILKLTEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5L8</accession>
    <name>A0A218P5L8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cation tolerance protein CutA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CutA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853341.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5L8"/>
    <dbReference type="GeneID" id="33314884"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01395"/>
    <dbReference type="OrthoDB" id="8015at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010038">
        <property type="term" value="P:response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053426">
        <property type="entry name" value="CutA_tolerance"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004323">
        <property type="entry name" value="Ion_tolerance_CutA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041095">
        <property type="entry name" value="dival_cat_tol_CutA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23419">
        <property type="entry name" value="DIVALENT CATION TOLERANCE CUTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23419:SF8">
        <property type="entry name" value="FI09726P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03091">
        <property type="entry name" value="CutA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="GlnB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="104" mass="12496" checksum="17FDA9203E6BC202" modified="2017-09-27" version="1">MEGIFVYTTFPDWESARKIVRELLERKLIVCANLREHEAMYWWEGKIEEGKEVGAILKTEVSKWKELREAIRELHPYEIPVIARIDLDKLNREYSEWMARVLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5M8</accession>
    <name>A0A218P5M8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GTPase HflX</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">GTP-binding protein HflX</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hflX</name>
        <name evidence="10" type="ORF">A3L08_01295</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">GTPase that associates with the 50S ribosomal subunit and may have a role during protein synthesis or ribosome biogenesis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer. Associates with the 50S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="6">May associate with membranes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. HflX GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853323.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5M8"/>
    <dbReference type="GeneID" id="33314864"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01295"/>
    <dbReference type="OrthoDB" id="10150at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="small_GTP"/>
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        <property type="entry name" value="GTP-BINDING PROTEIN HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GTPASE HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16360">
        <property type="entry name" value="GTP-bdg_M"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MMR_HSR1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hflx-type G" evidence="9">
        <location>
            <begin position="186"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="192"/>
            <end position="199"/>
        </location>
        <ligand>
            <name>GTP</name>
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        </ligand>
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        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
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        </ligand>
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        <location>
            <begin position="217"/>
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        </location>
        <ligand>
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        </ligand>
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        <location>
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        </location>
        <ligand>
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        </ligand>
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        <location>
            <begin position="238"/>
            <end position="241"/>
        </location>
        <ligand>
            <name>GTP</name>
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        </ligand>
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        <location>
            <begin position="306"/>
            <end position="309"/>
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        </ligand>
    </feature>
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        <location>
            <begin position="341"/>
            <end position="343"/>
        </location>
        <ligand>
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        </ligand>
    </feature>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        </source>
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        </source>
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        <source>
            <dbReference type="PIRSR" id="PIRSR006809-2"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51705"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ASJ06060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="436" mass="49875" checksum="3FFA2F6EA94E1465" modified="2017-09-27" version="1">MRAIGVIRKSRRERVSRAEFEELLRSAGYEVVAILEQNREEHPKYNIGRGKLEELRELVNEFKPDKVIFANKLSPSQAYNLWKELKVEVIDRWQLVLEIFEKRAHSTEAKLQVELASLQYEVPLVKEAIRRIKLGDRAGFKGMGEYQTQQYLKHIRYRMGRIRRELERVKADREVKRKRREEVGFILLALAGYTNAGKSTLLNALAGEEIEARNQMFTTLDTTTRRFKLSGKRVLVTDTVGFIDGLPPFIVEAFHSTLEEIVKADIILLVLDASEPWGEIRRKLLASLHVLRELKTLDKPMIVVLNKRDLTSEEDILDKAERIREIVEERGISVFRVVSISAKLGQLGELYDALEEVILTLPKYGAFEVIVKEPEKVPQVMALINAIGDVLAVEYGAETRIKAYVQTGMIKELIRLGVEIKRLDQSGEGKSLGDGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5N2</accession>
    <name>A0A218P5N2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01345</name>
    </gene>
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        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
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    <dbReference type="OrthoDB" id="103680at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="256"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="281"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="311"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="345"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="384"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="412"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="454" mass="50076" checksum="E3573E45F5FF4180" modified="2017-09-27" version="1">MRKVPVLIVLILLLIIPPVSAGELIYHPNREAFQAFLNSDSPYTVVSGNDDWARGWAYYVDERLHTLKPRGNGTLVLVGNVYNNGLMASLWNQTGLPKNASLLPAIIVLNNTILITGSENNIYLTERAFEGLWNPPDGSLVAFLLLAFFLMLLFLILLSNDDSHAGRFYALAFSLYVLWYLTAERPLFTEGFLMDMLSALKFTVGGLPDSPLSALMGAAFRFIPPIEENVVFIHWVLVLIILSFSFYLAPKKARELGFLVFGLAFVAPMFRSYLGHISGSALGLAGFVITLSVICNVTFSPEKWKALLQTAVLSLFTLLAIAINPYLVAIPLIFVIAFPKRHLRNYVYLVITGASAFLMYRAFGTPQGLPTGISANALSYLKDFLLNSGLAIATAVYAAANRKEIRMKGPTAFLFLTTVIYLPAAFFIPSLFPYSFVLLAALTVRLIHGLTPKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5R9</accession>
    <name>A0A218P5R9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 15">Fructose-1,6-bisphosphate aldolase/phosphatase</fullName>
            <shortName evidence="15">FBP A/P</shortName>
            <shortName evidence="15">FBP aldolase/phosphatase</shortName>
            <ecNumber evidence="6 15">3.1.3.11</ecNumber>
            <ecNumber evidence="15">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">fbp</name>
        <name evidence="16" type="ORF">A3L08_01465</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes two subsequent steps in gluconeogenesis: the aldol condensation of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GA3P) to fructose-1,6-bisphosphate (FBP), and the dephosphorylation of FBP to fructose-6-phosphate (F6P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 15">Homooctamer; dimer of tetramers.</text>
    </comment>
    <comment type="domain">
        <text evidence="15">Consists of a single catalytic domain, but remodels its active-site architecture via a large structural change to exhibit dual activities.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the FBP aldolase/phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="6 15"/>
    <dbReference type="EC" id="4.1.2.13" evidence="15"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853354.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5R9"/>
    <dbReference type="GeneID" id="33314898"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01465"/>
    <dbReference type="OrthoDB" id="5829at2157"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02067">
        <property type="entry name" value="FBP_aldolase_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002803">
        <property type="entry name" value="FBPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036076">
        <property type="entry name" value="FBPase_V_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041126">
        <property type="entry name" value="FBP_aldo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38341">
        <property type="entry name" value="FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE/PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38341:SF1">
        <property type="entry name" value="FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01950">
        <property type="entry name" value="FBPase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015647">
        <property type="entry name" value="FBPtase_archl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111249">
        <property type="entry name" value="Sulfolobus fructose-1,6-bisphosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 15" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 15" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="10 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="11 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 15" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Proton acceptor; for FBP phosphatase activity" evidence="15">
        <location>
            <position position="15"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor; for FBP aldolase activity" evidence="15">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with DHAP; for FBP aldolase activity" evidence="15">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="107"/>
            <end position="108"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="250"/>
            <end position="251"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ASJ06091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="375" mass="41658" checksum="FA209F3C99A47ECE" modified="2017-09-27" version="1">MAVGDKITLSVIKADIGGWPGHSRVHPQLVETAEEVLSKAVEDGTLIDFYVAYAGDDLQLIMTHKKGVDSPEIHGLAWDAFKAATEVAKELGLYGAGQDLLKDAFSGNVRGMGPGVAEMEITLRKSEPVVTFHMDKTEPGAFNLPIFRMFADPFNTAGLVIDPHMHMGFRFEVWDILKHKRVILNTPEEVYDLLALIGAKSRYVIKRVYPKEGHSISKDEPVAVVSTEKLYEVAGEYVGKDDPVAIVRAQSGLPALGEVLEPFAFPHLVSGWMRGSHNGPVMPVPMHQANPTRFDGPPRVVALGWQISPRGELVGPVDLFDDPAFDYARQKALEITEYMRRHGPFEPHRLPLEDMEYTTLPGVLKRLEERFEDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5S6</accession>
    <name>A0A218P5S6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Radical SAM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_01535</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5S6"/>
    <dbReference type="GeneID" id="33314912"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01535"/>
    <dbReference type="OrthoDB" id="49555at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034471">
        <property type="entry name" value="GDGT/MA_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034474">
        <property type="entry name" value="Methyltransferase_Class_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056488">
        <property type="entry name" value="Zn_ribbon_HMPTM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045702">
        <property type="entry name" value="rSAM_GDGT_ether"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43306">
        <property type="entry name" value="7,8-DIHYDRO-6-HYDROXYMETHYLPTERIN DIMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43306:SF1">
        <property type="entry name" value="7,8-DIHYDRO-6-HYDROXYMETHYLPTERIN DIMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23545">
        <property type="entry name" value="Zn_ribbon_HMPTM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00385">
        <property type="entry name" value="7_8-dihydro-6-hydroxymethylpte"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01100">
        <property type="entry name" value="methyltransferase_(Class_D)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="132"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="589" mass="68713" checksum="2AC37BAB677D7EA9" modified="2017-09-27" version="1">MAESVGEVPSGEKEFAESTRRIRDIIEFPEINEEEFENMLKGASRAYGGPLPHRTYSICPETRRVVPALVWEKDGKVWITKKCPEGMITDLYYEDVEMYYRFQKWKYDFKLMSANVENSGVNCPLDCGLCARHRSHTNLLNIVLTNRCNLSCWYCFFYAKEGQPIYEPTLEQIRMMLRNAKKEHPIGANAVQFTGGEPTIRDDLIEIIKIAKEEGYDHVQLNTDGIKLAFDPELVKKIREAGTNTLYLSYDGMTPQTNWKNHWEIPLIFENVRKAGGPGIVLVPTTIRNVNDHELGAIINFGLNHLDIVRGVNFQPISQVGRVPKKERQRFRITIPGAIKRIEEQTNGAIAKEDWYPIPIAGHIARFFEAFTGSRYYMTSHYCCGAATYVFLDRENKRVVPIPRFIDVEGFVEFLESKAEEIEQWKKLGRLQKLKLGAEIFMKFKSFYDEEYAPKGISVLELIKNAFMHGNYDALGKFHTNALFLGMMHFMDEYNYDVERVERCVIHYAMPDGRVVPFCTFNVIPELYRDKVQAQFSYTWEEWKALHPDWDYKKDKYFRSKEFVEKMRNSELYRKTYIDIEDYFGLNEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5S9</accession>
    <name>A0A218P5S9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Serine/threonine protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01675</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853390.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5S9"/>
    <dbReference type="GeneID" id="33314940"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01675"/>
    <dbReference type="OrthoDB" id="86092at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052396">
        <property type="entry name" value="Meiotic_Drive_Suppr_Kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37171">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE YRZF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37171:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE YRZF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="215" mass="24787" checksum="EECD742DCE7CC9EB" modified="2017-09-27" version="1">MFDHLISKAQLERFYAHLSSRGFAEIRPYAKGTTSLIFRAKLDEKNVIIKLQRPDSPRQNFAREAEIVKAIESFGITPPLVAYGKFEGLEYLIREFAEGEIIFYADLEKRHLFEIAEKTALLDRLGLDHGQIQGGKHIIIGKRVYLIDFEKANWRKPKNLTSAMAMIFLNDNYISRRVREKFGIDGTFLEEMKQELAEYKRTGRLSGLLALLSCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5V7</accession>
    <name>A0A218P5V7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853436.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5V7"/>
    <dbReference type="GeneID" id="33314990"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01925"/>
    <dbReference type="OrthoDB" id="101320at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="280" mass="30803" checksum="B5529A34600C973C" modified="2017-09-27" version="1">MKRYAVLFIAILLIGLVAGCLGRNSGETTPSGTTSSATQSITKKVETTTTTQTPKGPDLDELLKTVNSIRQFTYTSNATLRMLVTIEQGNTSETDNVTLNVLENGYMDYESWSAWINSTTVSLPDGAKTNMSRIVVDNITYIQSIVGWVKAEDTGASEIVWRYSIVGLAKEYLGEKPSSVENNGTLKLVYHIPDYRLRPFATVYFATSSETVVNVEDGRLELYFRDGQLVGGRLSFSVSSETDVNDPTLGEMKITQNGTWDETFKITSINEKKAVKAPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5W1</accession>
    <name>A0A218P5W1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA N6-adenosine threonylcarbamoyltransferase</fullName>
            <ecNumber evidence="9">2.3.1.234</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">N6-L-threonylcarbamoyladenine synthase</fullName>
            <shortName evidence="9">t(6)A synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">tRNA threonylcarbamoyladenosine biosynthesis protein Kae1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">kae1</name>
        <name evidence="11" type="ORF">A3L08_01975</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Kae1 likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>L-threonylcarbamoyladenylate + adenosine(37) in tRNA = N(6)-L-threonylcarbamoyladenosine(37) in tRNA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="9">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer. Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="9"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853446.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5W1"/>
    <dbReference type="GeneID" id="33315000"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01975"/>
    <dbReference type="OrthoDB" id="6818at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:tRNA N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd24131">
        <property type="entry name" value="ASKHA_NBD_Kae1_arch_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000038">
        <property type="entry name" value="Probable tRNA N6-adenosine threonylcarbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01446">
        <property type="entry name" value="Kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034680">
        <property type="entry name" value="Kae1_archaea_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017860">
        <property type="entry name" value="Peptidase_M22_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03722">
        <property type="entry name" value="arch_KAE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007174">
        <property type="entry name" value="PRK09605.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF14">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01016">
        <property type="entry name" value="GLYCOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Gcp-like" evidence="10">
        <location>
            <begin position="23"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="127"/>
            <end position="131"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="325" mass="35536" checksum="83AB46B814BD34EC" modified="2017-09-27" version="1">MIALGIEGTAHTLGIGIVTEKKVLANVFDTLTTEKGGIHPKEAAEHHARLLKPLLRKALETAGITMEDVDVIAFSQGPGLGPALRVVATAARALAIKYNRPIVGVNHCIAHVEITKMFGVKDPVGLYVSGGNTQVLALEGGRYRVFGETLDIGIGNAIDTFARELGIGFPGGPKIEKLALKGKRYIELPYAVKGMDLSFSGVLTEAVRKYRTGKYRVEDLAYSFQETTFSALVEVTERAVAHTGKEEVVLVGGVAANNRLREMLRIMTEDRGIKFFVPPYDLCRDNGAMIAYTGLRMYRGGVRFNIKDTVVKQKFRTDEVEVVWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5X9</accession>
    <name>A0A218P5X9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Bifunctional NAD(P)H-hydrate repair enzyme</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Nicotinamide nucleotide repair protein</fullName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="19">ADP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
                <ecNumber evidence="19">4.2.1.136</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="19">ADP-dependent NAD(P)HX dehydratase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="19">NAD(P)H-hydrate epimerase</fullName>
                <ecNumber evidence="19">5.1.99.6</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="18" type="primary">nnrE</name>
        <name evidence="17" type="synonym">nnrD</name>
        <name evidence="22" type="ORF">A3L08_02090</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14 19">Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="function">
        <text evidence="17">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="function">
        <text evidence="18">Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18 19">
            <text>(6R)-NADHX = (6S)-NADHX</text>
            <dbReference type="Rhea" id="RHEA:32215"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:64075"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 18 19">
            <text>(6R)-NADPHX = (6S)-NADPHX</text>
            <dbReference type="Rhea" id="RHEA:32227"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:64077"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 17 19">
            <text>(6S)-NADHX + ADP = AMP + phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32223"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17 19">
            <text>(6S)-NADPHX + ADP = AMP + phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32235"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18 19">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="18 19">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="17">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">In the C-terminal section; belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">In the N-terminal section; belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.136" evidence="19"/>
    <dbReference type="EC" id="5.1.99.6" evidence="19"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853467.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5X9"/>
    <dbReference type="GeneID" id="33315023"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02090"/>
    <dbReference type="OrthoDB" id="15148at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052855">
        <property type="term" value="F:ADP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052856">
        <property type="term" value="F:NAD(P)HX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110051">
        <property type="term" value="P:metabolite repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10260">
        <property type="entry name" value="YjeF N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01966">
        <property type="entry name" value="NADHX_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017953">
        <property type="entry name" value="Carbohydrate_kinase_pred_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030677">
        <property type="entry name" value="Nnr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004443">
        <property type="entry name" value="YjeF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036652">
        <property type="entry name" value="YjeF_N_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00197">
        <property type="entry name" value="yjeF_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592:SF0">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03853">
        <property type="entry name" value="YjeF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017184">
        <property type="entry name" value="Nnr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64153">
        <property type="entry name" value="YjeF N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01050">
        <property type="entry name" value="YJEF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51385">
        <property type="entry name" value="YJEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 17 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 18 19" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12 17 19" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 18 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10 17 19" id="KW-0520">NAD</keyword>
    <keyword evidence="8 17 19" id="KW-0521">NADP</keyword>
    <keyword evidence="6 17 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 18 19" id="KW-0630">Potassium</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjeF N-terminal" evidence="21">
        <location>
            <begin position="6"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="YjeF C-terminal" evidence="20">
        <location>
            <begin position="204"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="53"/>
            <end position="57"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="128"/>
            <end position="134"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="418"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="419"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR017184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="ASJ06203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="480" mass="52133" checksum="EF6F8F45AE622653" modified="2017-09-27" version="1">MRIEDVYIWDINAKWLGITPYQLMENAGAGVARVIEERFGKGLKIAVFSGTGNNGGDGFVTARHLSFENEVALFLVGDEAKIRSEEARHNWEILKGLDFVEIKVLKDSAYIRSLDLSGFDVIVDALLGAGTKGEPREPIRSAIEKINEYAGEVRIVSVDLPSGYPSKIRVKADFAVTFQWDKEEYEGFERVVVKIGYPRELYHLVGPGDAKFALRKRGEHKGQNGKLLVIGGSEDYFGAPYLASKAASYLVDLVYLAMPEYSAKRISDPDLILRPVEGRDFVPGHVDTLLKLAEKVDAIVIGPGLGLSDETKAFVREFVKHCAKPMVIDADGLKAVAEDLSVLRGKTFVLTPHAGEFRVLFGVGPEGPLAEKAELVMEKAGEIGGTILLKGAYDVISDGKTWKYNKTGNMGMTTGGTGDVLAGLVGALLALGNKPLRAASVGAFLNGLAGDMVKEELGENFTALEMAKKVPHTVKWALEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Y9</accession>
    <name>A0A218P5Y9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_02100</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853468.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5Y9"/>
    <dbReference type="GeneID" id="33315025"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02100"/>
    <dbReference type="OrthoDB" id="46231at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051081">
        <property type="entry name" value="HTH_MetalResp_TranReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33154:SF38">
        <property type="entry name" value="HTH ARSR-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33154">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ARSR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12840">
        <property type="entry name" value="HTH_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="185" mass="21773" checksum="44E26FC62CC67230" modified="2017-09-27" version="1">MREVLIITDPETVKVLSEETRFKILQLLRMRPMTISELSETLKRDRTTIYRHIKTLEKAGLVEELESQGNERIYSRSARLFLIKADPDESIEQFRQAYLQVEAEKLVQILEKAGFRVKDRGELVRLAKQVLDEIEIRSQPVVKRISQAEIELTEVELFHLLNMLVFMQSCELCEKARRARELMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Z0</accession>
    <name>A0A218P5Z0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Deoxyribose-phosphate aldolase</fullName>
            <shortName evidence="6">DERA</shortName>
            <ecNumber evidence="6">4.1.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">2-deoxy-D-ribose 5-phosphate aldolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Phosphodeoxyriboaldolase</fullName>
            <shortName evidence="6">Deoxyriboaldolase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">deoC</name>
        <name evidence="7" type="ORF">A3L08_01860</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes a reversible aldol reaction between acetaldehyde and D-glyceraldehyde 3-phosphate to generate 2-deoxy-D-ribose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>2-deoxy-D-ribose 5-phosphate = D-glyceraldehyde 3-phosphate + acetaldehyde</text>
            <dbReference type="Rhea" id="RHEA:12821"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:62877"/>
            <dbReference type="EC" id="4.1.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Carbohydrate degradation; 2-deoxy-D-ribose 1-phosphate degradation; D-glyceraldehyde 3-phosphate and acetaldehyde from 2-deoxy-alpha-D-ribose 1-phosphate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the DeoC/FbaB aldolase family. DeoC type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.4" evidence="6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853424.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5Z0"/>
    <dbReference type="GeneID" id="33314977"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01860"/>
    <dbReference type="OrthoDB" id="31145at2157"/>
    <dbReference type="UniPathway" id="UPA00002">
        <property type="reaction ID" value="UER00468"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004139">
        <property type="term" value="F:deoxyribose-phosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006018">
        <property type="term" value="P:2-deoxyribose 1-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009264">
        <property type="term" value="P:deoxyribonucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00959">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000044">
        <property type="entry name" value="Deoxyribose-phosphate aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00114">
        <property type="entry name" value="DeoC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011343">
        <property type="entry name" value="DeoC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028581">
        <property type="entry name" value="DeoC_typeI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00126">
        <property type="entry name" value="deoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10889">
        <property type="entry name" value="DEOXYRIBOSE-PHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10889:SF1">
        <property type="entry name" value="DEOXYRIBOSE-PHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01791">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001357">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01133">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="6">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with acetaldehyde" evidence="6">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="6">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="223" mass="24308" checksum="8D7909C548DDBEED" modified="2017-09-27" version="1">MNAREIARYIDHTNLKPYATEEDIKKLCDEAIQYGFYAVCVNPYRVKLAKDYLREKNADVKVASVIGFPLGATPTEVKVFEAKRAIEDGADELDMVINIGALKDKDYDYVRNDIAEVVRVAHEKGAKVKVIIETCYLTEEEKVKACELAKEAGADFVKTSTGFGTGGATVEDVRLMRKTVGPEMGVKASGGIRTYEQALAMIEAGATRIGTSSGVKIVEGASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P604</accession>
    <name>A0A218P604_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fe-S oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_01915</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853434.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P604"/>
    <dbReference type="GeneID" id="33314988"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01915"/>
    <dbReference type="OrthoDB" id="30736at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050377">
        <property type="entry name" value="Radical_SAM_PqqE_MftC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04085">
        <property type="entry name" value="rSAM_more_4Fe4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228:SF34">
        <property type="entry name" value="TUNGSTEN-CONTAINING ALDEHYDE FERREDOXIN OXIDOREDUCTASE COFACTOR MODIFYING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13186">
        <property type="entry name" value="SPASM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe4S-binding SPASM" evidence="1">
        <location>
            <begin position="225"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="310" mass="34940" checksum="5FCF325C23C5A210" modified="2017-09-27" version="1">MEKVAYDTAQAFGIALSPNVVSVAKPPWSDRPHSGRLERLILQLGSGKGRFSEVTGIPRSIGCIGNNSFILRREPLSVERVRELIKEFKLAGGRELWLTNYDSHDYLLSIAAYAAEIEVPEVYAVVLLDDVDEIRPIDGVRFIAELEYSPENVQRLEARDWLHGALVMVKGSDLDGLRGLKTTFPGEIYLDVLYPGSARKLDFNVIEMRRILNPTTERYHDCLAGTLAITADGYALPCPLLRNNIVADAKETSIKGILRKKRLKEFWRMTKDRIGTCSNCPFRYICHDCRALEYQATGEIDGLEYCPVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P627</accession>
    <name>A0A218P627_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Type II secretion protein F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_02185</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853484.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P627"/>
    <dbReference type="GeneID" id="33315042"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02185"/>
    <dbReference type="OrthoDB" id="12374at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.81.30">
        <property type="entry name" value="Type II secretion system (T2SS), domain F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042094">
        <property type="entry name" value="T2SS_GspF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35007">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35007:SF2">
        <property type="entry name" value="PILUS ASSEMBLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="290"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Type II secretion system protein GspF" evidence="8">
        <location>
            <begin position="186"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="35"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="354" mass="40257" checksum="826F9276B08B0858" modified="2017-09-27" version="1">MGVVRAITDFLERLGGKTIEVAEMPVRRIPQGTTIQERLRALKELQREIEVEKAETEREKELEEIIEWRKKEIQKPFSDRFAEAILRYFRGPVESLTASFKGLDQDLYRASILTSSDRYVALMLGVAIFAGIFGFIFAYLLYLPIDMSILVGFLGFVGGFFYMRYYPKMVWKRRVAEVERAMPYVLRHMASLLSAGVGVSEAILSVARADYGVISEEFELMLRDMRAGSSFEDALTKFDEKMGSENVSRVVKQILRAVKFGGNLSEILYKLAEDFAFEYRMKLVEYVQKVNGIAFIYMFLTIVMPTMFVVGILAGSVMARALIMPPETLAVVLLFAFPAISVIIVNMIKKGEPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P689</accession>
    <name>A0A218P689_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_02530</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853546.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P689"/>
    <dbReference type="GeneID" id="33315112"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02530"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="139" mass="15841" checksum="0CEACE83442760EE" modified="2017-09-27" version="1">MVSFEIRDGKLIITLWDSADMDIISALDQQMSSEFSWCYKVVRIDGEISKKPIFRDDTCDEECPVYGFSPLELTYLGKSGNTGIAWGGIKSVEAGPLTLWVDDDRNLVVSLNRTEHRQVRLDMPLPIERFTVIVLRKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6A1</accession>
    <name>A0A218P6A1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 20">Glutamine synthetase</fullName>
            <ecNumber evidence="4 20">6.3.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">A3L08_02695</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 20">
            <text>L-glutamate + NH4(+) + ATP = L-glutamine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="15">Binds 2 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Oligomer of 12 subunits arranged in the form of two hexagons.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 19">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17 18">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="4 20"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853578.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6A1"/>
    <dbReference type="GeneID" id="33315145"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02695"/>
    <dbReference type="OrthoDB" id="36124at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004809">
        <property type="entry name" value="Gln_synth_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027302">
        <property type="entry name" value="Gln_synth_N_conserv_site"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00653">
        <property type="entry name" value="GlnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43785">
        <property type="entry name" value="GAMMA-GLUTAMYLPUTRESCINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43785:SF12">
        <property type="entry name" value="TYPE-1 GLUTAMINE SYNTHETASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00180">
        <property type="entry name" value="GLNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51986">
        <property type="entry name" value="GS_BETA_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 19" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 20" id="KW-0436">Ligase</keyword>
    <keyword evidence="11 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS beta-grasp" evidence="21">
        <location>
            <begin position="11"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="GS catalytic" evidence="22">
        <location>
            <begin position="101"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="189"/>
            <end position="191"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="237"/>
            <end position="239"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="O-AMP-tyrosine" evidence="16">
        <location>
            <position position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR604809-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR604809-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR604809-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR604809-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU004356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="ASJ06316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="441" mass="49918" checksum="2FAF9D2B7638C7C5" modified="2017-09-27" version="1">MNDISTVLAGGEIKKPGFVQLVFVDINGVPKGMEIPIERYDEAVEDGISFDGSSIPGFQGIEDSDLMFKVDPETYTEVPWEGIARVYGHVYQNGKPYRADPRGVLRDALEELEKEGFKAYIGPEPEFYLFKKNGTWELQLPDGGGYFDLITLDRAREVRREIALYMQYLGLKPEVLHHEVGNAQHEINFRYDEALRTADNIVSFKYTVKAVAEMRGLYATFMPKPLYGFPGNGMHLHISLWKDGKNAFIGEDGLSETALHFMAGILKHAKALTALTNPTVNSYKRLVPGYEAPVYISWGYRNRSSLIRVPAFRGNGARIEYRCPDPGANPYLAFAAVLLAGLDGIKRRLEPGDYVETNVYEMKEEERERLGIDTLPGSLSEALDALRKDKVVRKSLGGAYGNFMKYKEAEWDAYTEYLESKGLPEDTKKVTEWELERYFHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6A7</accession>
    <name>A0A218P6A7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Potassium transporter TrkA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_02565</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a potassium transport system.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853552.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6A7"/>
    <dbReference type="GeneID" id="33315119"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02565"/>
    <dbReference type="OrthoDB" id="24929at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="entry name" value="Regulator of K+ conductance, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006036">
        <property type="entry name" value="K_uptake_TrkA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050721">
        <property type="entry name" value="Trk_Ktr_HKT_K-transport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43833">
        <property type="entry name" value="POTASSIUM CHANNEL PROTEIN 2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43833:SF5">
        <property type="entry name" value="TRK SYSTEM POTASSIUM UPTAKE PROTEIN TRKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00335">
        <property type="entry name" value="KUPTAKETRKA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="TrkA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0630">Potassium</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RCK N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="8">
        <location>
            <begin position="141"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="230" mass="25454" checksum="F1D0DF32DF6D15AF" modified="2017-09-27" version="1">MFVVIMGAGRVGFLVAKMLEEDGHDVTIIEMNKERAKELSLLINGLVIEGDATDPKTLEEANIKQADAFAALTGKDDANLLACILAKHLNPKIRTSLRIGNPKNRKIFEEVTDLKRYFDFVISPEEIAAEYISRNIVTPGFDRVLFPKEGAEIVRFNIDDNSDVAGKLVKDLKLPKDALIIAIYDEKGNLIIPSGDTKLPEKGQVIVFAKNNALEGVKGLLERKKHDNES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6J2</accession>
    <name>A0A218P6J2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9 10">L-aspartate oxidase</fullName>
            <ecNumber evidence="4 9 10">1.4.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">A3L08_03195</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the oxidation of L-aspartate to iminoaspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-aspartate + O2 = iminosuccinate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:25876"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
            <dbReference type="EC" id="1.4.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.16" evidence="4 9 10"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853664.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6J2"/>
    <dbReference type="GeneID" id="33315245"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03195"/>
    <dbReference type="OrthoDB" id="23539at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00326"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008734">
        <property type="term" value="F:L-aspartate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.100">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005288">
        <property type="entry name" value="NadB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00551">
        <property type="entry name" value="nadB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006254">
        <property type="entry name" value="PRK08401.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42716">
        <property type="entry name" value="L-ASPARTATE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42716:SF2">
        <property type="entry name" value="L-ASPARTATE OXIDASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0274">FAD</keyword>
    <keyword evidence="5 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 10" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="11">
        <location>
            <begin position="6"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal" evidence="12">
        <location>
            <begin position="423"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="NCBIfam" id="TIGR00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="466" mass="51040" checksum="468A0A9684F8091F" modified="2017-09-27" version="1">MTSVGIIGDGAAGLTAAVALARRGFDVTVIGRGFKNTNSYLAQAGIALPILDGDSLRAHFIDTVRAGKYLNDEEVVWNVLGKATEVYDFLSSIGLEFETNETEGGHSFHRVFTIRNETGKHMAKLLFMAAREAGVNFVEGFAEELAVREGRTYGVFLDGEILKFDATVIATGGFAGLFKYTAGSPLAIGLLIGDGVMKGAPARDLEFVQFHPTGYLGKDGVKLISEAVRGAGAKLVTEDGERFVNELSTRDIVARAIYMQMQNGKRVYLDTRSIEDFKKRFPQIYAFLRKDGIDPSKDLIPVAPIAHYTIGGIAVDLWYRTNVKGLYAIGEAASNGFHGANRLASNSLLECIVSGLEVARMIVREKPRLGEVPEVPYTFDSLGDINSLRELLWEHVGIVRTAEGLKAGLEKLEGIEADERLKLLARGVLECALAREESRGAHYREDFLVMRKEFERPSFFDGGCRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6P2</accession>
    <name>A0A218P6P2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">YhfC family intramembrane metalloprotease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853716.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6P2"/>
    <dbReference type="GeneID" id="33315304"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03495"/>
    <dbReference type="OrthoDB" id="86194at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="243" mass="26557" checksum="07A02CD3297757CC" modified="2017-09-27" version="1">MYPFPFTILGALLAWATVYAVGFRKQRWAEFILGLAAFFLAMIVQNPVQQLPLLGIGIRSNADVVARGTAFTVAVALWLGLAAGFVQEGVKYYLVRERRLREAVFVGLGFGVTEAVVIAIATAIPLITRGLSPEVPLLNALVSMGERYFTTLFHVGTAVLLAYYAQKGLGKRGLLYMIGLHTVLDTGAAYFQLAFFMEPRPVHTLNVLAYFLEAAVAVVGILVFAYGALKALSEPEVEERPIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Q5</accession>
    <name>A0A218P6Q5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AsnC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_03595</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853733.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Q5"/>
    <dbReference type="GeneID" id="33315324"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03595"/>
    <dbReference type="OrthoDB" id="14763at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012318">
        <property type="entry name" value="HTH_CRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43704">
        <property type="entry name" value="BSR5907 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43704:SF1">
        <property type="entry name" value="BSR5907 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00419">
        <property type="entry name" value="HTH_CRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="5"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="HTH crp-type" evidence="2">
        <location>
            <begin position="21"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="154" mass="17762" checksum="3372C245A772564F" modified="2017-09-27" version="1">MEEKTALTPRQLRLLRKFYEDGKTIEVHTVEKTQDELAEELGITRQALSNHLKVLKELGYIRTGRGFIDLTDKALDLLGEKKGDVFVFVRIEPTKRRNVYEAIKNLKIKKIYRVTGDIDLIIEADKTRLDEILEEISSLDGVKETITHIVLEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6R9</accession>
    <name>A0A218P6R9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Molybdate/tungstate import ATP-binding protein WtpC</fullName>
            <ecNumber evidence="8">7.3.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">A3L08_03660</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tungstate(in) + ATP + H2O = tungstate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35027"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46502"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">The complex is composed of two ATP-binding proteins (WtpC), two transmembrane proteins (WtpB) and a solute-binding protein (WtpA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ABC transporter superfamily. Sulfate/tungstate importer (TC 3.A.1.6) family.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.6" evidence="8"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853743.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6R9"/>
    <dbReference type="GeneID" id="33315337"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03660"/>
    <dbReference type="OrthoDB" id="18368at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902495">
        <property type="term" value="C:transmembrane transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901238">
        <property type="term" value="F:ABC-type tungstate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000042">
        <property type="entry name" value="Maltose/maltodextrin ABC transporter, ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050093">
        <property type="entry name" value="ABC_SmlMolc_Importer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42781">
        <property type="entry name" value="SPERMIDINE/PUTRESCINE IMPORT ATP-BINDING PROTEIN POTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42781:SF4">
        <property type="entry name" value="SPERMIDINE_PUTRESCINE IMPORT ATP-BINDING PROTEIN POTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="2"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="334" mass="37909" checksum="3308FC82F99A289C" modified="2017-09-27" version="1">MVRVELRGVIKKWDDFKLEIGELKVKDGEFLTLLGPSGCGKTTTLRMIAGFERPDRGEILFDGKPVNDLPPYERGIGIVFQDYALFPHMTVFKNIAFGLEMKRLPKPEIERKVRWALELVGLTGFENRYPEQLSGGQQQRVALARALVVEPEVLLLDEPLSNLDAKIRERLRGEIKRIQRELGITTIYVTHDQEEAMAVSDRIAVMNVGRVEQVGKPLELYYRPKTEFVARFLGLSNILELEAENGRACLNGLCFDVAGEGKVRVFFRPESVYIKSGDTAEVIDYELLPGRIRLKLGIEGEVILAERFLEELPFSVEEMPERVGIEVRSFSVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6W8</accession>
    <name>A0A218P6W8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">A3L08_03925</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="2"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853794.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6W8"/>
    <dbReference type="GeneID" id="33315390"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03925"/>
    <dbReference type="OrthoDB" id="6747at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00081">
        <property type="entry name" value="Hint"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1640.10:FF:000038">
        <property type="entry name" value="Cell division control protein 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002469">
        <property type="entry name" value="Cell division control protein 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000036">
        <property type="entry name" value="DNA replication licensing factor, MCM2/3/5 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.16.10">
        <property type="entry name" value="Hedgehog/Intein (Hint) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006142">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01443">
        <property type="entry name" value="intein_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF66">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14890">
        <property type="entry name" value="Intein_splicing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00379">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="Hedgehog/intein (Hint) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0651">Protein splicing</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="12">
        <location>
            <begin position="278"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="12">
        <location>
            <begin position="547"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="839" mass="94946" checksum="DB5515D97EB60C66" modified="2017-09-27" version="1">MEREDMIERYARFLRGYVDDEGREVYLNKLKDLLTLTPKRSLSIDWTHLNSFDPELAAELLENPEESILAAEDAIQIVLREQLLKEDEFKVHARFFNLPKTLLVKELGSEHINRFIQVEGIITRMSEVKPFVEKAVFVCKDCGNEMIRLQRPYENLVKPAKCDACGSRNIDLDVEKSRFINFQSFRLQDRPESLKGGQMPRFVDAILLDDLVDTALPGDRVLVTGILRVILEQKDKRPIFKKVLEVNHIEQLSKEIEELEISPEDEQKIRELAKRKDIVDAIVDSIAPAIWGHKTVKKGIALALFGGVQRTLPDGTKLRGESHVLLVGDPGVAKSQILRYVANLAPRAIYTSGKSSSAAGLTAAAVRDEFTGSWVLEAGVLVLADGGFACLHPDSRVLVDGKYQRIEDLFELSKSYKALSNGQVVDIQEKEMKVVALDLGSMKTKTSKATIIRRKPWKGELLRIRFRSGSEVTLTPDHLLVDGRTLEWKEAEKFRVGDSIMAVDEELNPRRDEIISIEKVPYEGYVYDLYVPGEHNFVAEGIIVHNCIDEFDKMSDRDRSAIHEALEQQSISISKAGITATLNSRTTVIAAANPKYGRFNRHKSLPEQLDLPPTLLSRFDLIFLLLDEPDEKVDASIAEHILKVRRGEAEAVTPKIPYDLLKKYIAYARKNVHPVLSREAMDEIKRYYVRMRKGFKRSGEDEGVQPIPVTARQLEALIRLSEAHARMRLSETVTREDARAAIQIIEEMIKNVATDEEGTLDISILEVGKSSKQINKIDRLIDIIKNLEGEGEFGAPEEKVLEAAKQAGIGTERDVRKLIDDLKRDARIYEPRAGFYRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6X0</accession>
    <name>A0A218P6X0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03825</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853775.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6X0"/>
    <dbReference type="GeneID" id="33315370"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03825"/>
    <dbReference type="OrthoDB" id="86016at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="95" mass="10873" checksum="918F05A08329FB32" modified="2017-09-27" version="1">MKIGELLELIDETIANLKIAVVANQQRALESPHTSYEFTQRAIELQEDLDDLMKAREMLAKLNPEDDAEEHFSREELGEFLKMLELLGSADAHVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6X4</accession>
    <name>A0A218P6X4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853787.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6X4"/>
    <dbReference type="GeneID" id="33315382"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03885"/>
    <dbReference type="OrthoDB" id="94130at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="136" mass="15443" checksum="F5F58A7C70D0C03E" modified="2017-09-27" version="1">MKLEKITLRNELFWKTGVAYLVLSVVLLAVEVMRGDTLFSLPNVFVGVVFIVMANRFRAVKLECDAETFFLIPDYSTSSVILKDSGGQVFLKRSFPLFEAEEIETPCGTVKIQAITHRFGKIELVIWKENKKITLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Y2</accession>
    <name>A0A218P6Y2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">Proteasome subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">20S proteasome alpha subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Proteasome core protein PsmA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">psmA</name>
        <name evidence="9" type="ORF">A3L08_03845</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 6">Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="4">The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 6">The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5 6">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853779.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Y2"/>
    <dbReference type="GeneID" id="33315374"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03845"/>
    <dbReference type="OrthoDB" id="9421at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03756">
        <property type="entry name" value="proteasome_alpha_archeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000004">
        <property type="entry name" value="Proteasome subunit alpha type-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00289_A">
        <property type="entry name" value="Proteasome_A_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019982">
        <property type="entry name" value="Proteasome_asu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03633">
        <property type="entry name" value="arc_protsome_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="8">
        <location>
            <begin position="10"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="227"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="260" mass="29076" checksum="4C6DC323FBF99D58" modified="2017-09-27" version="1">MAFVPPQAGYDRAITVFSPDGRLFQVNYAREAVKRGATAVGVKWKDGVVLAVEKRITSKLIEPSSYEKIFQIDDHIAAAPSGIIADARVLVDRARLEAQVYRLTYGEPVPLTVLVKKICDLKQAHTQYGGVRPFGAALLMAGVNDKPELYETDPSGAYFEWKAVAIGSGRNVAMAIFEEHYTDDIDREGAVKLAILALAKTLEEPSAEGIEVAYITTEDKRWKKLSKEDIEKYLAEILEEVKEEEVEEREEDYSELDQNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Y8</accession>
    <name>A0A218P6Y8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Translation factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853801.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Y8"/>
    <dbReference type="GeneID" id="33315398"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03970"/>
    <dbReference type="OrthoDB" id="100256at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16265">
        <property type="entry name" value="Translation_Factor_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029459">
        <property type="entry name" value="EFTU-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041207">
        <property type="entry name" value="tRNA_bind_PBP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14578">
        <property type="entry name" value="GTP_EFTU_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Elongation factor Tu-type" evidence="1">
        <location>
            <begin position="24"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="106" mass="11975" checksum="7BF6B4F4BAEDD84F" modified="2017-09-27" version="1">MGFLNRVFRRAKDDNPLEIVSREPVGKFRVSKTLRVMGRQVLIGEVLEGVVYPAYKVKAGRKVAIIYRIEKGHREVEFAAPGDMVALILEGEVEVGKNDEIEVYQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Z7</accession>
    <name>A0A218P6Z7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Aspartate aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_04020</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853810.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Z7"/>
    <dbReference type="GeneID" id="33315408"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04020"/>
    <dbReference type="OrthoDB" id="35685at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008453">
        <property type="term" value="F:alanine-glyoxylate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004760">
        <property type="term" value="F:L-serine-pyruvate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019265">
        <property type="term" value="P:glycine biosynthetic process, by transamination of glyoxylate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06451">
        <property type="entry name" value="AGAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000213">
        <property type="entry name" value="Putative serine--glyoxylate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024169">
        <property type="entry name" value="SP_NH2Trfase/AEP_transaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152:SF24">
        <property type="entry name" value="ALANINE--GLYOXYLATE AMINOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152">
        <property type="entry name" value="AMINOTRANSFERASE CLASS V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000524">
        <property type="entry name" value="SPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="8">
        <location>
            <begin position="46"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="385" mass="43037" checksum="477A3801A17D381F" modified="2017-09-27" version="1">MELRFDMQYEDAYREVYEMVKPKYKLFTAGPVACFPEVLAIMSVQMFSHRSAEAKEVHVDTLNRLKAFLEADKGEIILFPSSGTGFMEAAVRNTVPRGGKVLVTVIGAFGKRFAEVVEANGRKAVVFEKEPGQAVKPEELDDALRKNPDVHAVTITYNETSTGVLNPLPELAKVVHEHDKLLFVDAVSAMGGADIRFDEWGLDMIFASSQKAFGVPPGLAVAAVSERVFEIAEKMPERGWYFDLPLYKKFNEKKKGTPSTPPLPQIFGLNVVLRIVEKMGGKEKWLGMYRKRSEMIRNGVKEMGLGILAEPGYESPTITAVVVPEGMKGVDVYNAMRERGFELAKGYGSVAEKTFRIGNMGYMTFEDIEEMLDNLREVIEELKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P745</accession>
    <name>A0A218P745_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps9</name>
        <name evidence="7" type="ORF">A3L08_04250</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_055430131.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P745"/>
    <dbReference type="GeneID" id="74505993"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04250"/>
    <dbReference type="OrthoDB" id="52677at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000051">
        <property type="entry name" value="30S ribosomal protein S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00532_A">
        <property type="entry name" value="Ribosomal_uS9_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019958">
        <property type="entry name" value="Ribosomal_uS9_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_uS9_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001749">
        <property type="entry name" value="PRK00474.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03627">
        <property type="entry name" value="uS9_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569:SF16">
        <property type="entry name" value="RIBOSOMAL PROTEIN S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="108"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="119"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="135" mass="15272" checksum="B0AB32A542C86BC3" modified="2017-09-27" version="1">MKVIQTAGKRKTAIARATIREGKGRVRINHKPVEIIEPEIARFTIMEPLVLAGEEIVSKVDIDVKVEGGGFMGQAEAARVAIARALVEWTNDMNLKEKFMKYDRTMLVGDSRRTEPHKPNRSTKGPRAKRQKSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P755</accession>
    <name>A0A218P755_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Isopentenyl phosphate kinase</fullName>
            <shortName evidence="10">IPK</shortName>
            <ecNumber evidence="2 10">2.7.4.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">A3L08_04390</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the formation of isopentenyl diphosphate (IPP), the building block of all isoprenoids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>isopentenyl phosphate + ATP = isopentenyl diphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:33963"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:65078"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.26"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the isopentenyl phosphate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.26" evidence="2 10"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853872.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P755"/>
    <dbReference type="GeneID" id="33315482"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04390"/>
    <dbReference type="OrthoDB" id="15328at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102043">
        <property type="term" value="F:isopentenyl phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04241">
        <property type="entry name" value="AAK_FomA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024192">
        <property type="entry name" value="Fosfomycin_R_FomA-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040647">
        <property type="entry name" value="IPPK_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654">
        <property type="entry name" value="GLUTAMATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654:SF1">
        <property type="entry name" value="ISOPENTENYL PHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016496">
        <property type="entry name" value="Kin_FomA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6 10 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/glutamate/uridylate kinase" evidence="13">
        <location>
            <begin position="1"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="5"/>
            <end position="9"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="12">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR016496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR016496-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR016496-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ06615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="263" mass="28833" checksum="0A9A5CFE70E656BD" modified="2017-09-27" version="1">MIIVKIGGSVFSDKKGKPENFDVETVREIAREIANFYPREDFIVVHGGGSFGHPEAKKYGIRDGLPEDWESASLKRIGFTLTHQAMLRANAKFIEAFVAENLPAFSVSTSSVFITENGEVAYGDVEVIERLLELKFIPVLFGDVSIDLAKGIDILSGDQIITYLTKMLEPKKVIFLMDVDGIYDGKPGEGELVQNLSKEEIDALLDRLHCTAAGTDVTGGICNKLREAKKIAEHSEVWFVNGKVPGRLSGAIRGDGFGTRLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P781</accession>
    <name>A0A218P781_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Pyridine nucleotide-disulfide oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_04355</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P781"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04355"/>
    <dbReference type="OrthoDB" id="27922at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050260">
        <property type="entry name" value="FAD-bd_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429:SF3">
        <property type="entry name" value="NITRITE REDUCTASE [NAD(P)H]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="6">
        <location>
            <begin position="3"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="5">
        <location>
            <begin position="325"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="445" mass="48758" checksum="E8239B91CE17E9EC" modified="2017-09-27" version="1">MKYDVVVIGASAGGLTAAISAKKFYPDKTVLVIRKEEVGMIPCGIPYIFGTLKSVDDDVLPVERFLKPLGVDLLTDEVVEIDPKRKVLKTRSGREVAWEKLVIATGSRPAKPNFPGVELEGIYTISKDYEYLKRLREKIEEAERIVIVGGGFIALEVGDEIRKLGKEVTLVVRSRLLRSSFDPEFSEMIEERLKEVGVNIIHGQVEAFEGSGKVEKIRLLDGKEVPADLVILSTGYKPNVELAIKAGLKVTRYGIWTDEYMRTSHPDIFAVGDCVEHRDFFTGKPYGLMLASTATFEARIAGANLFKLQIVRENRRTIGVYSTHVAGLTLAAAGLTEEAAKKEGFEVIVGYGKGPDRHPAKFPDTSMVTVKLIFSRDRGAILGAQIAGGKSVGEMINILALAIQKRLTASELYTLQIATHPLLTASPVGYQILQAAEDALAKLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P789</accession>
    <name>A0A218P789_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Phosphoglycolate phosphatase</fullName>
            <shortName evidence="5">PGP</shortName>
            <shortName evidence="5">PGPase</shortName>
            <ecNumber evidence="5 6">3.1.3.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_04575</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the dephosphorylation of 2-phosphoglycolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>2-phosphoglycolate + H2O = glycolate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14369"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29805"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58033"/>
            <dbReference type="EC" id="3.1.3.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal SPP-like hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.18" evidence="5 6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853902.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P789"/>
    <dbReference type="GeneID" id="33315519"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04575"/>
    <dbReference type="OrthoDB" id="120822at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01427">
        <property type="entry name" value="HAD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07514">
        <property type="entry name" value="HAD_Pase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01419">
        <property type="entry name" value="GPH_hydrolase_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006382">
        <property type="entry name" value="PGPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01487">
        <property type="entry name" value="Pglycolate_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002245">
        <property type="entry name" value="PRK01158.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01482">
        <property type="entry name" value="SPP-subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10000:SF8">
        <property type="entry name" value="HAD SUPERFAMILY HYDROLASE-LIKE, TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10000">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="10"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="NCBIfam" id="TIGR01487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="243" mass="26510" checksum="F6F56F9944614B9C" modified="2017-09-27" version="1">MGRIKAISLDIDGTITYRDRTLSIDALKAIRLAERLGVPVMLVTGNSVPFAEAAAVFIGTSGPVIAEDGGALSLKGEGTMRKRVFLTDMDEEWILWSELKKRYPEAELSYSTMERKAGLVITRKVPVEAVRELIAELGLNLIAVDSGFAIHIKKPWINKGTGIEKACEFLGISPKEVAHVGDGENDLDAFRVVGYRVAIAQAPESLKEKADYVTEKPYGDGGAEAVMHILKKFGYLGEESLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P797</accession>
    <name>A0A218P797_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Glutamate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">A3L08_04525</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homohexamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 11">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853894.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P797"/>
    <dbReference type="GeneID" id="33315509"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04525"/>
    <dbReference type="OrthoDB" id="6425at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01076">
        <property type="entry name" value="NAD_bind_1_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000003">
        <property type="entry name" value="Glutamate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053388">
        <property type="entry name" value="GLPV_dehydrogenases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014362">
        <property type="entry name" value="Glu_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040817">
        <property type="entry name" value="GdhA_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF13">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000185">
        <property type="entry name" value="Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="12">
        <location>
            <begin position="184"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="10">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="419" mass="46577" checksum="7B1EF023703EF633" modified="2017-09-27" version="1">MVEIDPFEMAVKQLERAAQFMDISEEALEWLKKPMRIVEVSVPLEMDDGSVKVFTGFRVQHNWARGPTKGGIRWHPAETLSTVKALATWMTWKVAVVDLPYGGGKGGIIVDPKKLSEREKERLARSYIRAIYDVISPYTDIPAPDVYTNPQIMAWMMDEYEAISRRKVPSFGIITGKPPGVGGIVARMDATARGASYTVREAAKALGMDLKGKTIAIQGYGNAGYYMAKIMSEEHGMKVVAVSDSKGGIYNPDGLNADEVLEWKKKNGTVKDFPGATNITNEELLELDVDVLAPSAIEGVITKDNADKIKAKIVAELANGPTTPEADEILHEKGVLIIPDFLCNAGGVTVSYFEWVQNITGDYWDTETTRAKLDKKMTKAFWDVYNTHKEKGINMRDAAYVVAVQRVYDAMKWRGWVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P798</accession>
    <name>A0A218P798_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ArsR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_04640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853915.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P798"/>
    <dbReference type="GeneID" id="33315532"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04640"/>
    <dbReference type="OrthoDB" id="85663at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="1">
        <location>
            <begin position="4"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="70" mass="8366" checksum="1E51077B6C32657E" modified="2017-09-27" version="1">MKNVKVLEALNDGPKTIEEIAEKTGIKPMEVRRYLLRFVEQGKVESYQKEGKLFWKIKEKSEEEEEFKYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7A7</accession>
    <name>A0A218P7A7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="17 19">Reverse gyrase</fullName>
            <ecNumber evidence="17">5.6.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">rgy</name>
        <name evidence="25" type="ORF">A3L08_04650</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25 26">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process, increasing the linking number in steps of +1. Binds to single-stranded DNA, transiently cleaves and then rejoins the ends, introducing a positive supercoil in the process. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate. Involved in rewinding DNA strands in regions of the chromosome that have opened up to allow replication, transcription, DNA repair and/or for DNA protection.</text>
    </comment>
    <comment type="function">
        <text evidence="17">Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process, increasing the linking number in steps of +1. Binds to single-stranded DNA, transiently cleaves and then rejoins the ends, introducing a positive supercoil in the process. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate. Probably involved in rewinding DNA strands in regions of the chromosome that have opened up to allow replication, transcription, DNA repair and/or for DNA protection.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17 19">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="17">Binds 1 or 2 zinc ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 17">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="17">Introduction of positive supercoils requires the cooperation of both domains. The helicase-like domain probably does not directly unwind DNA, but more likely acts by driving ATP-dependent conformational changes within the whole enzyme. A beta hairpin in the 'latch' region of the N-terminal domain plays a regulatory role in the enzyme, repressing topoisomerase activity in the absence of ATP and preventing the enzyme from acting as an ATP-independent relaxing enzyme; it also helps to coordinate nucleotide hydrolysis by the ATPase domain with the supercoiling activity of the topoisomerase domain.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="17">This enzyme is the only unique feature of hyperthermophilic bacteria/archaea known and seems to be essential for adaptation to life at high temperatures. It may play a role in stabilization of DNA at high temperatures.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">In the C-terminal section; belongs to the type IA topoisomerase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15 17">In the N-terminal section; belongs to the DEAD box helicase family. DDVD subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.-" evidence="17"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853917.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7A7"/>
    <dbReference type="GeneID" id="33315534"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04650"/>
    <dbReference type="OrthoDB" id="30963at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160097">
        <property type="term" value="F:reverse gyrase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd17924">
        <property type="entry name" value="DDXDc_reverse_gyrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18798">
        <property type="entry name" value="SF2_C_reverse_gyrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03361">
        <property type="entry name" value="TOPRIM_TopoIA_RevGyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.510.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="entry name" value="Topoisomerase I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="entry name" value="Topoisomerase I, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01125">
        <property type="entry name" value="Reverse_gyrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005736">
        <property type="entry name" value="Reverse_gyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034142">
        <property type="entry name" value="TOPRIM_RevGyr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040569">
        <property type="entry name" value="Znf_Rg"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01054">
        <property type="entry name" value="rgy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43505">
        <property type="entry name" value="REVERSE GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43505:SF1">
        <property type="entry name" value="REVERSE GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17915">
        <property type="entry name" value="zf_Rg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="Prokaryotic type I DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52039">
        <property type="entry name" value="TOPO_IA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52037">
        <property type="entry name" value="ZF_RG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52036">
        <property type="entry name" value="ZF_RG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="20" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 17" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 17 19" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14 17 19" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 17 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 17 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 17 19" id="KW-0799">Topoisomerase</keyword>
    <keyword evidence="9 17 19" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 17 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RG N-terminal-type" evidence="23">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="22">
        <location>
            <begin position="93"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Toprim" evidence="21">
        <location>
            <begin position="642"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IA-type catalytic" evidence="24">
        <location>
            <begin position="821"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Topoisomerase I" evidence="17">
        <location>
            <begin position="638"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="260"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="17">
        <location>
            <position position="963"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00043976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU004026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS52036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS52039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="ASJ06661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="1228" mass="140995" checksum="302793C4C6F3E4FC" modified="2017-09-27" version="1">MKAIYREMCPNCLGRISDERLVEKNPCDECLDNPVHSDSYFELVTSVRNALQVKGTLKEWERIYSLEKNLREVEDFFEKATGFTFWSAQRTWVKRLLKGRSFSIIAPTGMGKSTFGAFMAVWHATRGKKSYIVVPTTPLVIQTVRKIQAIAGKAGVEVNLAYYHGNLRKKEKEEMLAKITGGDYDILVTSAQWMARKFDEVLKDRHFDFIFVDDVDAFLKASKNIDRSLYLLGFNDEVIGKAWEIIRLKKQMSKYLNGRAQDREEMLKELNESIAKLQREIEEFKSNNDIGIMIIASATGSARGDRIKLYRELLGFEVGSGRSALRNVVDSYLKPTKDIKEHVEELLTMLGKGGIIFTPIDQGLGYAEELVNYLRERGFKVELVSSKNRKAIERFENGEADYLVGSATYYGSLVRGLDLPHLIRYAVFTGVPKFRFSIDLERPTIYRALGLLSEVMDFLSDEDRKQAEKLHARLRRLIRNIPQFELLKIEEALAEGLPIENEFHNHVLGVFRELVEFLRRVLHDEEVLKKLAEDPFVSLTEEGGKWYIEIPDVRTYIQATGRTSRLFAGGITKGLSVLLVDNEKVFNGLVRQMRWRFTEFKMVPFEELDLDEVLKQIDEDREKVRLVMEGKISAKVKDLVKSALMIVESPNKARTIANFFGQPSKTRIGDLVAYEVSIGNMMLTILASGGHMFDLVTNEGYHGVLIDEKDGMMRFIPVYDTIKRCRDCGHQFVDWEDKGVCPRCGSTNVRDALENVRAMRELAQEVDEILIATDPDTEGEKIAWDIRNVLSPYTPNIKRIEFHEVTRPAIMRAIQEARDVNEGRVNAQIVRRIEDRWIGFELSQELQRVFENRNLSAGRVQTPVLGWIIERYKEFSESETYFLGLTLENGLQVTVEIGKDGKNVEPPEYVTVEEVHLEEREFNPSPPYTTDAMLKDASTFLKLSAPETMRLAQDLFEAGLTSYHRTDSTHVSNTGIEIAKEYITQEIGEEYFKPRPWGEEGTHEAIRPTRPIDTGRLMQLVRDGVIQLPKNLTRNHYRLYDMIFRRFMTSQMKSAKLLMERAVIDAGVGKAELEGYVEVIEDGWTKLRSPPFRQLPRLEEGARLKVVEAKKWKAPKVSLYSQGDIIALMKERKIGRPSTYAKILETLIKRYYVLETRGRKKLVPTDQGIKVYHYLISKYKELVSEEKTRELEGVMDLIEENGVNYQEVLGELYREIMEHMGVGKGAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7D8</accession>
    <name>A0A218P7D8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA primase DnaG</fullName>
            <ecNumber evidence="9">2.7.7.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dnaG</name>
        <name evidence="12" type="ORF">A3L08_04865</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. Also part of the exosome, which is a complex involved in RNA degradation. Acts as a poly(A)-binding protein that enhances the interaction between heteropolymeric, adenine-rich transcripts and the exosome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ssDNA + n NTP = ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate.</text>
            <dbReference type="EC" id="2.7.7.101"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Forms a ternary complex with MCM helicase and DNA. Component of the archaeal exosome complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the archaeal DnaG primase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.101" evidence="9"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853952.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7D8"/>
    <dbReference type="GeneID" id="33315577"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04865"/>
    <dbReference type="OrthoDB" id="8643at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01029">
        <property type="entry name" value="TOPRIM_primases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1360.10:FF:000010">
        <property type="entry name" value="DNA primase DnaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00007">
        <property type="entry name" value="DNA_primase_DnaG_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050219">
        <property type="entry name" value="DnaG_primase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020607">
        <property type="entry name" value="Primase_DnaG_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034154">
        <property type="entry name" value="TOPRIM_DnaG/twinkle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003108">
        <property type="entry name" value="PRK04031.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30313">
        <property type="entry name" value="DNA PRIMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30313:SF2">
        <property type="entry name" value="DNA PRIMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13662">
        <property type="entry name" value="Toprim_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56731">
        <property type="entry name" value="DNA primase core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="9" id="KW-0271">Exosome</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2 9" id="KW-0639">Primosome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Toprim" evidence="11">
        <location>
            <begin position="200"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="321"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="321"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="471" mass="53143" checksum="493A72B5FDEA6D64" modified="2017-09-27" version="1">MKRKKTVLHHILTEKQKFEKRKEGDGMSAKDEFGTTKYVIYAEFEANGIVERPDVVGAIFGQTEGLLGDDLDLRELQKTGRIGRIRVEVHTKAGKTYGTITVPSSLDRVETAILAAALETIDRVGPAEAKIKVLRIEDVRATKRKYIIERAKEILEGLMEQEIPETQELTEEVKKAVRAKELIEYGPEKLPAGPHVPFSDSIIVVEGRADVLNLLKHGIKNAIAVEGTSVPETIIKLSKERIVTAFTDGDRGGELILKELLQVADVDYVARAPEGKEVEELTKKEIVKSLRSKVPAEQVITEIFYKGRNFYDVIKETEKAREEKKEEKKPTPVPVPAPAQVPAQRHVEERKPERPERPEPKKEEKIIRPIQQSRPSELEEFGSFIEKVKSARESTAILLDKDKNVIAEIPVRELTNQLKERKDVYAVVFNGVITQRLIDTVSESGVKYLIGARKYNVVRRPINLKIVTFAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7F4</accession>
    <name>A0A218P7F4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_04965</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853968.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7F4"/>
    <dbReference type="GeneID" id="33315597"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04965"/>
    <dbReference type="OrthoDB" id="102132at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="219" mass="24202" checksum="5DD151438EFC8340" modified="2017-09-27" version="1">MERLTAVLALVLIVLGAVGAWYLAGGGQTIHHTSAQVVKAKVLVRLGTMDCYSYSQNMTVSYGNVTIQSHADGGLNNGTYYFHGTRDEMEWWGTIKDHHLVEKVVGSGETKEIETNLTDEELSAMMLYDPVKLALRALGSSEDVQISASWITCNFTLPETEGGAHKTFSGTIKVRFDESYRPLKVVVDGKISYEGKTLRRVSFSADVKNECSTPEWENE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7I7</accession>
    <name>A0A218P7I7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter ATP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">A3L08_05170</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Part of an ABC transporter complex. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Probably part of an ABC transporter complex. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853999.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7I7"/>
    <dbReference type="GeneID" id="33315638"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05170"/>
    <dbReference type="OrthoDB" id="18209at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006824">
        <property type="term" value="P:cobalt ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000224">
        <property type="entry name" value="Energy-coupling factor transporter ATP-binding protein EcfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005876">
        <property type="entry name" value="Co_trans_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050095">
        <property type="entry name" value="ECF_ABC_transporter_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01166">
        <property type="entry name" value="cbiO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43553:SF24">
        <property type="entry name" value="ENERGY-COUPLING FACTOR TRANSPORTER ATP-BINDING PROTEIN ECFA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43553">
        <property type="entry name" value="HEAVY METAL TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="2"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="268" mass="29791" checksum="F989D9EDFF9F2E65" modified="2017-09-27" version="1">MIELEDVHFSYSGREVLRGISLAIERGEIFGLLGPNGAGKSTLILHLNGILKPKRGKVLVDGLDPAKNPREVRRKVGIVFQDPNDQLFSPTVFEDVAFGPYNLGLRGEELRKRVLNALELVGMVEYLNRDTKELSFGEKKRIAIATVLAIEPEIIVFDEPFANLDFRGKKLMRELILGLKNQGKTVILASHEAEYLSLCDRIALMDGGRIVTVGTPKEVLGNPDLLREHNLDVPPLAELFLSLDLPVPGSVEEGKKLLEEWKAGRIRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7J9</accession>
    <name>A0A218P7J9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">mannose-1-phosphate guanylyltransferase</fullName>
            <ecNumber evidence="2">2.7.7.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">cpsB</name>
        <name evidence="12" type="ORF">A3L08_05180</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>alpha-D-mannose 1-phosphate + GTP + H(+) = GDP-alpha-D-mannose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="EC" id="2.7.7.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the mannose-6-phosphate isomerase type 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.13" evidence="2"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854001.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7J9"/>
    <dbReference type="GeneID" id="33315640"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05180"/>
    <dbReference type="OrthoDB" id="5825at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004475">
        <property type="term" value="F:mannose-1-phosphate guanylyltransferase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02213">
        <property type="entry name" value="cupin_PMI_typeII_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02509">
        <property type="entry name" value="GDP-M1P_Guanylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000046">
        <property type="entry name" value="Mannose-1-phosphate guanylyltransferase (GDP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000032">
        <property type="entry name" value="Mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049577">
        <property type="entry name" value="GMPP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006375">
        <property type="entry name" value="Man1P_GuaTrfase/Man6P_Isoase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001538">
        <property type="entry name" value="Man6P_isomerase-2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054566">
        <property type="entry name" value="ManC/GMP-like_b-helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051161">
        <property type="entry name" value="Mannose-6P_isomerase_type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01479">
        <property type="entry name" value="GMP_PMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46390">
        <property type="entry name" value="MANNOSE-1-PHOSPHATE GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46390:SF1">
        <property type="entry name" value="MANNOSE-1-PHOSPHATE GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22640">
        <property type="entry name" value="ManC_GMP_beta-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01050">
        <property type="entry name" value="MannoseP_isomer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="9">
        <location>
            <begin position="2"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="MannoseP isomerase/GMP-like beta-helix" evidence="11">
        <location>
            <begin position="290"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-6-phosphate isomerase type II C-terminal" evidence="10">
        <location>
            <begin position="345"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF22640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="464" mass="52725" checksum="C985F846086B8D8B" modified="2017-09-27" version="1">MKTLILAGGKGTRLWPLSRELMPKQFVRFLDGESLFQKTVKRALKFSKPGEIFIVTNRKYKFRVLDDLSELGVEVPEENLLLEPVGKNTLPAIYWGAKRIEETFGDSIAAVLPSDHLIDAGEAYERAFRNAEKLAREHLVTFGIKPTKPHTGYGYIRPGEALREGDDILGYRVGEFKEKPDLETAKRYVEEGYYWNSGMFAFNTGVFLDEVKKHAPEVAKAFEEESIEKAYELAPDISIDYGVMEKTDKAAVVPLNTYWNDLGSFDAIYDVLEKDEDGNAVVMKGKKTYHIGVNSKNNLIMAERLTATVGVEDLIIIDTDDALLVAHRGEGQRVKEVYKRLKERNDERALVHRTAYRPWGSYTVLEEGERYKIKRLTVLPGKKLSLQMHYHRSEHWVVVRGTAKVRVGDEEILLRPGESTFIPAGVIHRLENPGKVVLEVIETQIGEYLGEDDIVRFEDDFGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7L0</accession>
    <name>A0A218P7L0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Osmotically inducible protein OsmC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05230</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854011.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7L0"/>
    <dbReference type="GeneID" id="33315650"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05230"/>
    <dbReference type="OrthoDB" id="106754at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003718">
        <property type="entry name" value="OsmC/Ohr_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036102">
        <property type="entry name" value="OsmC/Ohrsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34352">
        <property type="entry name" value="PROTEIN YHFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34352:SF1">
        <property type="entry name" value="PROTEIN YHFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02566">
        <property type="entry name" value="OsmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82784">
        <property type="entry name" value="OsmC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="136" mass="15637" checksum="1FD5C956CF399492" modified="2017-09-27" version="1">MERLEYKAELEWDGNVGSTAKVREFTFKIDTNTDGRNAGPNPTEYLLSAIGGCLTVNWGRLIKKMRLDVEGFKVEVRGWRTRDEPQLREITYKITVFTNEPERKILRVKELAEKYGTVFNTVGAEKINGEVEIVRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7M1</accession>
    <name>A0A218P7M1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854036.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7M1"/>
    <dbReference type="GeneID" id="33315676"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05360"/>
    <dbReference type="OrthoDB" id="89477at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="97" mass="10784" checksum="83DA6115A9A31AD2" modified="2017-09-27" version="1">MIISKPCVTMKGIVISGYSWERPIKIDLAKTAQCLKEKGYTVKKLIPDMMLIVEMEGFEVSVYPSGKIIIKLLDDANRGREMATVVYDCAGILEVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7M4</accession>
    <name>A0A218P7M4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH-type transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_05280</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the GbsR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854020.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7M4"/>
    <dbReference type="GeneID" id="33315660"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05280"/>
    <dbReference type="OrthoDB" id="101229at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052362">
        <property type="entry name" value="HTH-GbsR_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026282">
        <property type="entry name" value="MJ1563"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38465">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR MJ1563-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38465:SF1">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR MJ1563-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006707">
        <property type="entry name" value="MJ1563"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="5">
        <location>
            <begin position="7"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR006707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="158" mass="18229" checksum="0222CEB44D4C677D" modified="2017-09-27" version="1">MAVSKRESRRFVEIVAQLLGRWGYSHTDGKIYAHLLLSERPLTISELAEATHLSRSSVSVSLSRLTRDYLVTYRKEGKTKYFSAVPAFLEKFLQQPKDILEREVRPLKEIIGRLAEKAESEEARTRFEGILNDLSTLECVLERIIKLEEQESDCIRSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7N7</accession>
    <name>A0A218P7N7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MATE family efflux transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_05425</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854049.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7N7"/>
    <dbReference type="GeneID" id="33315689"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05425"/>
    <dbReference type="OrthoDB" id="214119at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13142">
        <property type="entry name" value="MATE_like_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048279">
        <property type="entry name" value="MdtK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052031">
        <property type="entry name" value="Membrane_Transporter-Flippase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43549:SF2">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN NORM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43549">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN YPNP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="62"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="406"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="437"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="473" mass="51438" checksum="F2E5A2281E7CF5BE" modified="2017-09-27" version="1">MERGKLLRMREEILNGPIEKTLLKLAYPLIVNNLVQVLYNITDTFWLGKLGREALAAPGTSWPIIGTLMALGMGFATAGFALVGQYIGAGNYERANRSAGALYSLMLLFSTATAIISLAILPYALRFMKVTPNVYPYAKAYAQVVFAGVPLSFSFMAFSALMRASGDTKTPVKISMLTVGMNIVLDPIFIFVLGLGVEGAAIATVLSNGTGAIIGAKILTSGKAGLHLTRETMKPDFGFYRKIFHVGLPSAVGQSADSFGFVVLTRIIYGYGDVVYAAYTITTRLVNFITSIARGVSMAMGTMIAQNVGAENYGRAKKIAERAMVINFLIASFAIVVIGLFRVPVFRVFLDDPAVIAQSEYVLKYFLISVPFFNGIFVVVTRTFSSAGHTKKSMFLSMLRLWGLRIPLSYAFGYVGAITVLGLTIPLAKLFGFTSKGVFFGMGMSNFLAALVALAWFMRGTWMRRIIEDESKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7P7</accession>
    <name>A0A218P7P7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF835 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_05495</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854062.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7P7"/>
    <dbReference type="GeneID" id="33315703"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05495"/>
    <dbReference type="OrthoDB" id="85946at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008553">
        <property type="entry name" value="DUF835"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33531:SF7">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33531">
        <property type="entry name" value="RUBRERYTHRIN SUBFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05763">
        <property type="entry name" value="DUF835"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="DUF835" evidence="2">
        <location>
            <begin position="229"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="367" mass="41399" checksum="09941188E47D2631" modified="2017-09-27" version="1">MTWDTIIAYLNIISRWALFIAVLHKAYQTREKGWALLTTAFFIDVMDIEDYVLGPLGMTINSRAYEVASTIPNFFIAILLVWGAIHLKYGSSKLKHVLYISGVTVVAYVWIFLLATDLIESPSIRASVPSLLFGLASIYFGRVLLNYVVSSHWIEKLFPWGLILLGALNLIYPIARFIDWFAPIGFLLGAVFRFIAAIGALKFVFYPVSPVTSETELDIKPGAFMLRSKEEVLSKFGEFYAKPGTVLVTREDIKELSQRAHPETLVFWITRSAEGKLSESPTVYATSPTRIDILTDLIAKAVSQGYHVVYIDAFEYLMLENSFENAVKFLLNVKDRVISSGGTLILVINPDTLTERQRKIIEREFGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Q6</accession>
    <name>A0A218P7Q6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854055.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Q6"/>
    <dbReference type="GeneID" id="33315696"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05460"/>
    <dbReference type="OrthoDB" id="101940at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="255"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="296" mass="31944" checksum="D1FBEF8BAFF669BD" modified="2017-09-27" version="1">MGAVDAFVKTFSLILANKKLYLLALILTLILAPMGAYLVPNEIPFEQNQTSIQKGSVIVEEYGSLLNEDEMDVFLQLMKGLAVYLLISIVLSSIFEYSVAKGALAYLAGEERALRDLILDGVRHFPGVFVINVVYSLIAFTFIGIAFIPIVAGVLTLPVGGVLILVGIVLLILIGALVTSLSALAIPLYADRGSIGAAFEAFGLVFRNVLSSMGFGLLVWVGIFGIAMVSAPIAFITELFLTSDAGTYVSALLQAPFNALLSTFIWVAGVAFYRELQRMEELKKVDEELAELGMDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Q7</accession>
    <name>A0A218P7Q7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Gamma carbonic anhydrase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05555</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854072.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Q7"/>
    <dbReference type="GeneID" id="33315715"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05555"/>
    <dbReference type="OrthoDB" id="10940at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd04645">
        <property type="entry name" value="LbH_gamma_CA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047324">
        <property type="entry name" value="LbH_gamma_CA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050484">
        <property type="entry name" value="Transf_Hexapept/Carb_Anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13061">
        <property type="entry name" value="DYNACTIN SUBUNIT P25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13061:SF29">
        <property type="entry name" value="GAMMA CARBONIC ANHYDRASE-LIKE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="174" mass="18840" checksum="C7BFFCF9A18D163E" modified="2017-09-27" version="1">MAVYELGGKKPKVHETAFIDETASVIGDVVLEEKTSVWPSAVLRGDIEQIYIGCCSNVQDNVSIHTSHGLPTRVGKYVTIGHNAVVHGATIDDYTIIGMGAIILDGAKIGKHVIIGAGALVPPGKEIPDYSLVVGVPGKVVRQLSEEEIEWTKKNAEIYMELAEKHLQSRKKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7R4</accession>
    <name>A0A218P7R4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-directed RNA polymerase subunit Rpo11</fullName>
            <ecNumber evidence="6">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">DNA-directed RNA polymerase subunit L</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpo11</name>
        <name evidence="6" type="synonym">rpoL</name>
        <name evidence="8" type="ORF">A3L08_05590</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the archaeal Rpo11/eukaryotic RPB11/RPC19 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854078.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7R4"/>
    <dbReference type="GeneID" id="33317877"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05590"/>
    <dbReference type="OrthoDB" id="24205at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06927">
        <property type="entry name" value="RNAP_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00261">
        <property type="entry name" value="RNApol_arch_Rpo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009025">
        <property type="entry name" value="RBP11-like_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008193">
        <property type="entry name" value="RNA_pol_Rpb11_13-16kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022905">
        <property type="entry name" value="Rpo11-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002235">
        <property type="entry name" value="PRK01146.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I,II,III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946:SF28">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13656">
        <property type="entry name" value="RNA_pol_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01154">
        <property type="entry name" value="RNA_POL_L_13KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 6 8" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RBP11-like dimerisation" evidence="7">
        <location>
            <begin position="13"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="95" mass="10977" checksum="D7B951EFBC5D23F2" modified="2017-09-27" version="1">MKIEVIKREENVLEFYLEGEDHTFANLLNEVLHENKHVTFAGYTIEHPVLMARKPKFRVVTDGKVTPEKALEEAAQKIFDRARAVLEAWNAALKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7S5</accession>
    <name>A0A218P7S5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phenylalanine--tRNA ligase alpha subunit</fullName>
            <ecNumber evidence="11">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Phenylalanyl-tRNA synthetase alpha subunit</fullName>
            <shortName evidence="11">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">pheS</name>
        <name evidence="13" type="ORF">A3L08_05675</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 2 magnesium ions per tetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854095.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7S5"/>
    <dbReference type="GeneID" id="33315739"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05675"/>
    <dbReference type="OrthoDB" id="372178at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00496">
        <property type="entry name" value="PheRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000095">
        <property type="entry name" value="Phenylalanine--tRNA ligase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00282">
        <property type="entry name" value="Phe_tRNA_synth_alpha2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004529">
        <property type="entry name" value="Phe-tRNA-synth_IIc_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022917">
        <property type="entry name" value="Phe_tRNA_ligase_alpha_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00468">
        <property type="entry name" value="pheS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003210">
        <property type="entry name" value="PRK04172.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF40">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="12">
        <location>
            <begin position="248"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>L-phenylalanine</name>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="383"/>
            <end position="385"/>
        </location>
        <ligand>
            <name>L-phenylalanine</name>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="424"/>
        </location>
        <ligand>
            <name>L-phenylalanine</name>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>ligand shared with heterodimeric partner</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="449"/>
        </location>
        <ligand>
            <name>L-phenylalanine</name>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="501" mass="57359" checksum="849B967732E8C4C5" modified="2017-09-27" version="1">MELSYQEKLTLIKLGEARKLKFEELVEKTGLDQVAVMRSVLGLQAKGLAKLHESSEKVAVLTETGREYARIGLPEWRALRVLKEKGKVTLDDLSGVLSEDELKPIVGLLRKEGWANVRKEEGKLILEITERGLNAEERPIDKALKLLAEKGSVPAKEIEKLISVKELKRRKIAEEGTVTERTVEITPAGEELVKKGIELKREVSVLTPELIKSGKWREVEFRRFDIKAPVRRIYPGKKQPYRAFLDKIRRRLIEMGFIEMTVESLIETQFWNFDALFQPQNHPAREWTDTYQLKYPKSGHLPGEELVERVKAAHERGGDTGSRGWGYVWSPERAMLLMPRAHGTALSGRQLAKGVEIPGKYFTIQRVFRPDVLDRTHLIEFNQVDGFVVGEDLNFRHLLGILKRFAVEIAGAKKVKFLPDYYPFTEPSVQMSAYHPELGWVEFGGAGIFREEMTKALGIDAPVIAWGIGIDRLAMFKLGIDDIRYLFSYDLRWLREARLVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7U0</accession>
    <name>A0A218P7U0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phenylalanine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="12">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Phenylalanyl-tRNA synthetase beta subunit</fullName>
            <shortName evidence="12">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">pheT</name>
        <name evidence="14" type="ORF">A3L08_05670</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="12"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854094.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7U0"/>
    <dbReference type="GeneID" id="33315738"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05670"/>
    <dbReference type="OrthoDB" id="10073at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.10:FF:000011">
        <property type="entry name" value="Phenylalanine--tRNA ligase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000132">
        <property type="entry name" value="Phenylalanine--tRNA ligase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.40.10:FF:000003">
        <property type="entry name" value="Phenylalanine--tRNA ligase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="entry name" value="Phenylalanyl-trna Synthetase, Chain B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00284">
        <property type="entry name" value="Phe_tRNA_synth_beta2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004531">
        <property type="entry name" value="Phe-tRNA-synth_IIc_bsu_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022918">
        <property type="entry name" value="Phe_tRNA_ligase_beta2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00471">
        <property type="entry name" value="pheT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947:SF0">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE BETA CHAIN AND LEUCINE-RICH REPEAT-CONTAINING PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56037">
        <property type="entry name" value="PheT/TilS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="9 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B5" evidence="13">
        <location>
            <begin position="278"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ06844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="573" mass="65716" checksum="F07F8AEC19EC0739" modified="2017-09-27" version="1">MPKFDVSKSDLERLIGKSFTVEEWEDLFLYAKCELDDVWEENGEIYFKADSKDTNRPDLWSAEGIARQIKWALGMKRGLPKYEVEKSDVVVYVDEKLKDIRPYGVYAIVEGLHLDEEALKQMINLQEKVALTFGRRRREVAIGIFDFDKVKPPIYYRAAEKTEKFVPLGFTDELTLEEILEKHEKGKEYGHLIKDKPYYPLLVDSEGKVLSMPPIINSEITGRVTTETKNVFVDVTGWDLNKIMLALNVVVTALAERGGKIKSVKVVYPDFEIETPDLTPKPFEVELDYIRKLAGLELSDDEIKDLLERMMYEVELEGGKARLLYPAFRDDIMHARDVLEDVLIAYGYNEIEPEEPKLAVQGRGDKFVEFEDAVRELMVGFGLQEVMTFNLTNREAQYSRMNLPCEEGEECDGYFNHPPAELVEIENPISPKWSALRNWLLPSLLDFLSQNTHEEYPQRVFEVGKATLIDESRETKTVSESKLAVALAHPRVTFTEAKEILDSVMRHLGFEYELEEVEHPSFIPGRAGKIVVEGKAIGIIGEIHPAVLENWGLEMPVAGFELFLRPLYTEPYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7W2</accession>
    <name>A0A218P7W2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Flavin-dependent thymidylate synthase</fullName>
            <shortName evidence="1">FDTS</shortName>
            <ecNumber evidence="1">2.1.1.148</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">FAD-dependent thymidylate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Thymidylate synthase ThyX</fullName>
            <shortName evidence="1">TS</shortName>
            <shortName evidence="1">TSase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thyX</name>
        <name evidence="2" type="ORF">A3L08_05860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NADPH and FADH(2) as the reductant.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADPH + H(+) = dTMP + (6S)-5,6,7,8-tetrahydrofolate + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:29043"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="2.1.1.148"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="1">Binds 4 FAD per tetramer. Each FAD binding site is formed by three monomers.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thymidylate synthase ThyX family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.148" evidence="1"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854131.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7W2"/>
    <dbReference type="GeneID" id="33315776"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05860"/>
    <dbReference type="OrthoDB" id="18918at2157"/>
    <dbReference type="UniPathway" id="UPA00575"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050797">
        <property type="term" value="F:thymidylate synthase (FAD) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20175">
        <property type="entry name" value="ThyX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01408">
        <property type="entry name" value="ThyX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003669">
        <property type="entry name" value="Thymidylate_synthase_ThyX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036098">
        <property type="entry name" value="Thymidylate_synthase_ThyX_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02170">
        <property type="entry name" value="thyX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34934">
        <property type="entry name" value="FLAVIN-DEPENDENT THYMIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34934:SF1">
        <property type="entry name" value="FLAVIN-DEPENDENT THYMIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02511">
        <property type="entry name" value="Thy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69796">
        <property type="entry name" value="Thymidylate synthase-complementing protein Thy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51331">
        <property type="entry name" value="THYX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="1" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Involved in ionization of N3 of dUMP, leading to its activation" evidence="1">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <note>ligand shared between neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="80"/>
            <end position="83"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="83"/>
            <end position="85"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <note>ligand shared between neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="91"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <note>ligand shared between neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="165"/>
            <end position="167"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <note>ligand shared between neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <note>ligand shared between neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="245" mass="28979" checksum="0C1AA593A1F59FE1" modified="2017-09-27" version="1">MNDKIRVTLVNYTKKPLETVTWAALISYWDGWETEAFERMGEKDVEMHLPRVLGYGHESILEHATLTFAIEGCSRVCSHQLVRHRLASYTQQSQRYIKLNPNDVEETFVIPESVKEKPELYQKWKEFMKNAIELYEETYKAGIHQEDARFILPQAVRTKIVVTMNLRELKHFLGLRACERAQWEIREIAWKMLEEIAKNEELRPIIKWAKLGPRCIQIGYCPEGELMPPGCWKRTREKWKNVAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Z4</accession>
    <name>A0A218P7Z4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0284 protein A3L08_05945</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05945</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0284 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854148.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Z4"/>
    <dbReference type="GeneID" id="33315793"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05945"/>
    <dbReference type="OrthoDB" id="9136at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008939">
        <property type="term" value="F:nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02439">
        <property type="entry name" value="DMB-PRT_CobT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01086">
        <property type="entry name" value="UPF0284"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003200">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002805">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036087">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00303">
        <property type="entry name" value="nicotinate mononucleotide-dependent phosphoribosyltransferase CobT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003368">
        <property type="entry name" value="PRK04447.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003372">
        <property type="entry name" value="PRK04447.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38811">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38811:SF1">
        <property type="entry name" value="UPF0284 PROTEIN SLL1500"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02277">
        <property type="entry name" value="DBI_PRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52733">
        <property type="entry name" value="Nicotinate mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase (CobT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="334" mass="35930" checksum="25966498CBD89CDD" modified="2017-09-27" version="1">MESLFLLVLGNAEISTVPGISVAGATPELTKLTPVADAEYLFHERPLTIDVIPVTPEGHPTPAIITKAAKELANFPVLVVRGGTYLAPLVPHVHISNAVGRDFRKGPALPEFGEIIKRAKLFGEELNKLPIKELVIGESTPGGTTTAQAVLWAFGYDAKTSSASPDNPQSLKGMVITEAFQRAGIEKGQLRDNPLEALRQFGDPMMATVVGLSLGFRKDIVLAGGTQMLAVAALLKALDEDLSRFMIATTKWVVKDRSATFLDTAKEIGVITYAADLDFSESKFKGLRDYERGYVKEGVGAGGATWLAIKAGFSPEEVSEKVEALYRRLMGMKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P805</accession>
    <name>A0A218P805_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HEAT repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06120</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854177.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P805"/>
    <dbReference type="GeneID" id="33315827"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06120"/>
    <dbReference type="OrthoDB" id="103602at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="17022" checksum="DB3C4766C7C62899" modified="2017-09-27" version="1">MQEWYRSRALYEAVMKLVESGKINEALAMADGIPDRVIRSKAFSNIAVEVAKSGRDYSDALERAIKAALDIDSREESTKALMSLAFEFLNMGNPEEALRIAGYITDLSNKSKVEAEVALALAKRGNVSEAMEIINGIMDDDVKTWTMSRLASQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P847</accession>
    <name>A0A218P847_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Arginine--tRNA ligase</fullName>
            <ecNumber evidence="10">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Arginyl-tRNA synthetase</fullName>
            <shortName evidence="10">ArgRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">argS</name>
        <name evidence="14" type="ORF">A3L08_06285</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>tRNA(Arg) + L-arginine + ATP = L-arginyl-tRNA(Arg) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10 11">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="10"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854207.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P847"/>
    <dbReference type="GeneID" id="33315860"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06285"/>
    <dbReference type="OrthoDB" id="372102at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07956">
        <property type="entry name" value="Anticodon_Ia_Arg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00671">
        <property type="entry name" value="ArgRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000008">
        <property type="entry name" value="Arginine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.70:FF:000008">
        <property type="entry name" value="Arginine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000190">
        <property type="entry name" value="Arginine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="entry name" value="Arginyl tRNA synthetase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00123">
        <property type="entry name" value="Arg_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005148">
        <property type="entry name" value="Arg-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002447">
        <property type="entry name" value="PRK01611.3-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956:SF5">
        <property type="entry name" value="ARGININE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="ARGINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03485">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM01016">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55190">
        <property type="entry name" value="Arginyl-tRNA synthetase (ArgRS), N-terminal 'additional' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arginyl tRNA synthetase N-terminal" evidence="13">
        <location>
            <begin position="8"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="DALR anticodon binding" evidence="12">
        <location>
            <begin position="520"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="10">
        <location>
            <begin position="133"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ06958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="642" mass="73607" checksum="7AB3DB40447B17ED" modified="2017-09-27" version="1">MGYGEVKERVRLILQEVLEEMLNEAGKEWSGEITFDETPSIELGDFATTVSFQLARVFRKAPKLIAEEIVERLNGKLPEEIADVKAVNGYINFYLDYDRFGKELVSEILEKGASYGESGIGKGKKVIVEHTSVNPTKPLHMGHARNSVLGDTMARIMRKLGYTVEVQNYIDDLGVQFAQVLWGYLNLKEEFERIEAELREKGLKEDFIDHVMGLLYVEVNKRIEENPEVDKEVRELMKKLEEGDNEIAEIGRKLAERVVRAQMLTTYRMGITYDLLSWESDIMKSGIFNEAYELIEANENFFWAEEGKYKGAFVMDLRKLFPDMKNPFLVLRRSDGTATYTGKDIAYHLWKFGKVKADMLYKPWDRKDDHETWTTAPDGKEMPGKFGRADIVINVIGAEQKHPQMAIKYALQLLGFEDSAKNFHHLAYEHVVRPEGSFSGRKGTWVGFTVDEVLNEAVQRARELVEQKNPNLSEEEKDTIAEAVGVGAVRFNLVKYSPDKIITFRWDDVLNFEGESAPYIQYAHARCASIMRKAEENGIDIDWKSLLEKADFSKLTNREKELVKLLARFPEVVEQAGRDIKPHLIPAYLNELASLFNRFYMDHPVLKAEEGIVEERLLLVLAVKQVLRNALELLGIEAPERM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P882</accession>
    <name>A0A218P882_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NADH dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_06190</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854189.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P882"/>
    <dbReference type="GeneID" id="33315841"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06190"/>
    <dbReference type="OrthoDB" id="15253at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052561">
        <property type="entry name" value="ComplexI_Subunit1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001694">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1/FPO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43359">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43359:SF1">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00146">
        <property type="entry name" value="NADHdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="225"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="291"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ06940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="309" mass="33387" checksum="23CC0A3D303EA224" modified="2017-09-27" version="1">MIETVLKALFILLYATFVGFMFMGIIRIVTARIHRRVGPPIYQPILDTIKLLSKKSNITHGLIYDFGILYAVGATILAVMFIPLGSIGVLRAYGDLVLITFLLEIPMLGIMFAAMSSGNPYAGIGAQRALLTMLAIQVPLGFAIVALAEYYGTFSTYQIVMAQQVSGWSIAHLPLLLAAIAYDLVLQAMFGKEPFDIMIAPGEISLGPMVEFGGKHMGLLNIQHAIALFAETLFFSNIFLGGGVVTAFSSSVLNTIASLGVLLVKQIAVLLVATFMAAIFPRFTIDQAAKFYWKWPTIIAALGAILASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P883</accession>
    <name>A0A218P883_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Formate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_06505</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854247.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P883"/>
    <dbReference type="GeneID" id="33315904"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06505"/>
    <dbReference type="OrthoDB" id="2837at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16374">
        <property type="entry name" value="DMSOR_beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050954">
        <property type="entry name" value="ET_IronSulfur_Cluster-Binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43177:SF5">
        <property type="entry name" value="ANAEROBIC DIMETHYL SULFOXIDE REDUCTASE CHAIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43177">
        <property type="entry name" value="PROTEIN NRFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13247">
        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12800">
        <property type="entry name" value="Fer4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="2"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="69"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="164" mass="18395" checksum="AE3ED4E280108ADF" modified="2017-09-27" version="1">MKKVFIDFRKCIGCHSCEAACAREHNGKPNIFIVQTSELMMMSFNCRHCENAPCMLVCPARALYRDEDGAVRVKYQECIGCMLCSIACPFGTPRFDERLKVMVKCDLCANRRAEGKLPACVETCPMDALILATEEEIVEMKLKEAAVRREEFIRKVGGIMGCGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P888</accession>
    <name>A0A218P888_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_06540</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P888"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06540"/>
    <dbReference type="OrthoDB" id="91075at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="84" mass="9570" checksum="DE1A9A6210E8F096" modified="2017-09-27" version="1">MRAYLLRRGRSLGFQNEVSRVLDHFESSALVEEVICEISPETASLLEKGVFDIVAFPREDREVVKKHLETIKKGEITVGWVPDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P895</accession>
    <name>A0A218P895_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Hydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_06520</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P895"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06520"/>
    <dbReference type="OrthoDB" id="15253at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052561">
        <property type="entry name" value="ComplexI_Subunit1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001694">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1/FPO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43359">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43359:SF1">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00146">
        <property type="entry name" value="NADHdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="233"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="292" mass="31596" checksum="24BAC94BEBB99F8C" modified="2017-09-27" version="1">MNVHDAAPIIVPLLVPFLDGVARKVRAVIQKRVGPSIAQSWYDLLSFLRVECNSPVDSLAFRLAPYIAFASAIGMLLFLPFGEKAPLGFKGDIIAFIYVFTMFSAAVVMGALSVPSSYTSAGASREVTISIVFKPLFAVVIGIFAIKTGALTIEGMASALKPSISVLGAYLLLVYLTYVEAGFVPYDIAEAETELLGGPLAEYSGRLFAIMLWAFQIKRFAMIWLLASMLVLPFVGGITALLFQCAAFALIFLGMVVYEAMNARYRIDHAVRNGAIALTLGVFILVIAWTGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8B9</accession>
    <name>A0A218P8B9_9EURY</name>
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        <submittedName>
            <fullName evidence="3">Coenzyme F420 hydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_06600</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
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            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854264.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8B9"/>
    <dbReference type="GeneID" id="33315923"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06600"/>
    <dbReference type="OrthoDB" id="42371at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="entry name" value="Cytochrome-c3 Hydrogenase, chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018194">
        <property type="entry name" value="Ni-dep_hyd_lsu_Ni_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43600:SF1">
        <property type="entry name" value="COENZYME F420 HYDROGENASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43600">
        <property type="entry name" value="COENZYME F420 HYDROGENASE, SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="HydB/Nqo4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00507">
        <property type="entry name" value="NI_HGENASE_L_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0533">Nickel</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
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    <feature type="binding site" evidence="2">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
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        </location>
        <ligand>
            <name>Mg(2+)</name>
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        </ligand>
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        <location>
            <position position="380"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
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        </ligand>
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        <location>
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        </location>
        <ligand>
            <name>Fe cation</name>
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        </ligand>
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        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
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        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601501-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="392" mass="43747" checksum="F54B15D3B97701F6" modified="2017-09-27" version="1">MGEIELGKVCRISGDAKLIMYEEGGEVQDALFISTAPVRGFEKMVIGKSPFFVVEAVMRICGLCHASHGIAAAEAIENAIGVTPPRNGVLLREALGLINRIQSHLLEFLMVVPDLVVPEEREKLILELIDFHTKIGDFLLKLGGAMTHPPNITVGGINPVPKWSVFNNLKARLPKMLRSWNELAHLLVDEDIQTDVASELREKKHDNRYLSSGLFYGDRFNIDVGKIKLIPYYEFRKDDPSSKESTTMIAMYKGKPVEVGPRARLSTYREKSYSTLYDLHTARVDDVSIALHRLSSILDSLDMKEPFRNQNIVFGPGKGVGVYEAPRGTLVHIVELGEEGRTLYSRIVVPTMFNIPVIEEAVKGLSVKAAEAVVRLYDPCIPCTTHVVRLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8E8</accession>
    <name>A0A218P8E8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cation:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_06575</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8E8"/>
    <dbReference type="GeneID" id="33315918"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06575"/>
    <dbReference type="OrthoDB" id="371891at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050586">
        <property type="entry name" value="CPA3_Na-H_Antiporter_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003918">
        <property type="entry name" value="NADH_UbQ_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42703:SF1">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER SUBUNIT D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42703">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01437">
        <property type="entry name" value="NUOXDRDTASE4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="462"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="7">
        <location>
            <begin position="130"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="510" mass="55468" checksum="D526B71432FFCB55" modified="2017-09-27" version="1">MNLLILPVIPMAFAFVLPLLSTVIRNRRFIFGYALFVTGITLLMGGELFRQVYSSELPLTYSFGGWKAPVGIIYEVDRFGAVMVLVTAALMFLTAVYSVRYLEREDGVEWFYTLYLGLEAGLLGIFMTGDAFNLFVMLEVTAISSYGLVMFYTEEGYPAYSGLRYAIISSIGTTLYFLALVLIYRGLGTVNFADISAKLHGIEFPISTPLNSIVPILAISMALITWAFTIKAAIMPNHFWLPGAHSAAPSPVSAVLSGLVVNAGSYGFMRFLSLIYVPEVSRIGDIICTLLLILGAISALLSSIAMTVQDDVKRIVAYSTILNMGYLAMAVGVNSEAGTAGAVFHMVNHAIAKALLFLAVGVFIHAAGSRKLDELKGLGKNMPFTTVSLAIATMSLVGLPPTNVFFSKLLLYRAYIEKSPVLVAVLLLSSLFALVSYMRVLYWLWVKKPDEKREAHEPRSMVAVLVILAIACLVIGVLSPILVDRVVNPAVAQLKDVEGYIRAALTTGVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8G9</accession>
    <name>A0A218P8G9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_06960</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8G9"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06960"/>
    <dbReference type="OrthoDB" id="37176at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904680">
        <property type="term" value="F:peptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08509">
        <property type="entry name" value="PBP2_TmCBP_oligosaccharides_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.76.10">
        <property type="entry name" value="Dipeptide-binding Protein, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.105.10">
        <property type="entry name" value="Dipeptide-binding Protein, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290:SF82">
        <property type="entry name" value="ABC-TYPE DIPEPTIDE_OLIGOPEPTIDE TRANSPORT SYSTEM, PERIPLASMIC COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PERIPLASMIC BINDING COMPONENT OF ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="656"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="domain" description="Solute-binding protein family 5" evidence="2">
        <location>
            <begin position="93"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="679" mass="76511" checksum="5D08CBD9752676CD" modified="2017-09-27" version="1">MVSKKKISGMFLLLVFVGSFWGLQPVSAQISLPREDTVFVTGAQWSPASTWNLFSPSQTWGTYTSGGFMYLPLFQYIAGLNVWVPIIGESFELVNETTLIIHLRPEAKWSDGEPITAEDVVYTFELSKEVGSGPAAGSEPYIGDVKAIDPHTVQFTIGPENNLPMFLLYALQFAPAPKHIIEQVHQKVGNQIIEWRNCGGTCDDVISTDSGDVTINVPQVVSGPYKLYYFDELRIVYERIDDWWGKDIFGLPSPKYVVHRIYLSNEQALLDLRQGNVDWSGIFIPNVGNFKDIGTFYKGKPYFRPGAFVVLYFNHREPVLQDPNLRKAIAYAINYQEVLTKAFYDYSPQPSMSLVFTVFPHYRTWLNTTLAQEYLGNPEGKMPYDPERAKQILAQAGYKDVDGDGFLETPSGEKIELNIIIPTGWTDWMIAADLIAHNLQEIGLNVVANPVDYGQYWGMINGAGYTLALGWTNSPSFYHPWDTYRYVLDPRLTPPTANWGFYNNSKALELLVEAAKAKSSEELMKYYTEIQKLIYEDVPVIPIAYSVQWYAYSEKYWTGWPNQDNPWWTEVAPYREYSLPLWLLFGLSKKGEPVSPPQWAKPKDEGGILIPNKEVLSQLSGAFVPEVNITETFTTSSTSETETQPQPQAPASSSRFIVGILGIVIAVVVIVIGARKLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8J3</accession>
    <name>A0A218P8J3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_07160</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854366.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8J3"/>
    <dbReference type="GeneID" id="33316035"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07160"/>
    <dbReference type="OrthoDB" id="100793at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051598">
        <property type="entry name" value="TSUP/Inactive_protease-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43701">
        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN MJ0441-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43701:SF2">
        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN YJNA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="133"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="245" mass="25563" checksum="8FD0D603B6334AFF" modified="2017-09-27" version="1">MQTALLSMVSLIAGFIGSLMSGGSIIIFSLLTFLDLPVQTAVGTLKVAITALTFVSSVTYYRGGAVEVESAPYLVASSILGALAGSYFFASLSERTAGMVSLLFLFAGVYVSLKGEPEGLIFTVKKAHRLGISAAGFLIGVYIGILGIASTLIVIAALRAFFGMDMLQANGTAKILIFFNNLTAAVVYGLGGNIDYALVAPLLVPVCIGAWIGARAAMKLGGEKLRWVYLLIGSATALKLLSELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8M6</accession>
    <name>A0A218P8M6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">dolichyl-phosphooligosaccharide-protein glycotransferase</fullName>
            <ecNumber evidence="6">2.4.99.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Oligosaccharyl transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">A3L08_07280</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>an archaeal dolichyl phosphooligosaccharide + [protein]-L-asparagine = an archaeal dolichyl phosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.</text>
            <dbReference type="EC" id="2.4.99.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the STT3 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.21" evidence="6"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854388.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8M6"/>
    <dbReference type="GeneID" id="33316059"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07280"/>
    <dbReference type="OrthoDB" id="82393at2157"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004576">
        <property type="term" value="F:oligosaccharyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003674">
        <property type="entry name" value="Oligo_trans_STT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041530">
        <property type="entry name" value="OST_IS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048858">
        <property type="entry name" value="OST_P1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041152">
        <property type="entry name" value="OST_P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872:SF1">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT STT3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18246">
        <property type="entry name" value="OST_IS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21618">
        <property type="entry name" value="OST_P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18235">
        <property type="entry name" value="OST_P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="121"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="145"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="387"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="417"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="441"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="471"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="Oligosaccharyltransferase insert" evidence="19">
        <location>
            <begin position="626"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="domain" description="Oligosaccharyl transferase peripheral 1" evidence="20">
        <location>
            <begin position="724"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="domain" description="Oligosaccharyltransferase peripheral 2" evidence="18">
        <location>
            <begin position="823"/>
            <end position="955"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF18235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF18246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF21618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ASJ07137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="965" mass="109782" checksum="F6ADE499CE1A6AB6" modified="2017-09-27" version="1">MVKTDVKEKQKKKEVGASPSRYYSIFKGYGLPFIALLLAYLGFRLRNITSNYKTFLDPDTFFHYEMYRQAITEWIPKYFAYAEPPTGIKATGYLGLYTVQAAFYKITHALFGWSELQAFKVWPPFVGAMTVIAVYLLGRKLHSDWAGIWGAAFMAFSYASFTKTMSGNNRGEGPFMMFFLFAVYFLLVYLDEKEWNWKKVLGAVMFLVTSVLYMGVWTGSNFGVGILLLVAGITVVVFFTFGMMETLRDFVRDYFTLYGLSLLLGLGVSYTGFIGIKGFLIFALEAFIALSALVVVMLYGERLGLNYSDKKHRFGTILAIAVVGFLAFYGYFGRDLLKFMGAAYQSNPLYQTVAELARTSWGYIKDSFSIHYVYNANLRQYVGKDGALFLLSLAGFVIVLSRFILKLIKQDLSGYKEIFIVAYYFGSLYLLLSAVRFIFQASGTVVLLAGIVMAEIFIYVENMKENMGTKALYAILLIFLLIIPYVEARDVKSVAVSYSKSQGSVPADWVNVLNWLKENSNPLDSATSWWDYGYWIESSLLSQRRSATDGGHAYDRRYIVADFFSHYGNEAEQDFEAWELNYMITWQRDIYKFNAISYLGGAIDYYEYGHVPMFQLIPKQYIQYVNESGKTVVYVGTSQGAYQPVMTLDLSRGQTIPGRGDIPYVLYVFGDYGMLAYKNVAFSNFVRLAFQLPYSYEPWDAQKLFANFKPTYSNGGVSVYQFRPFAVYRIDKYQNSTWVPFYSTMSGGKLPLGEQKLRLWISAFGRDVKNATLVFEAYNGTTLVKKEVLAENLNINYLNETPVEVSLVVPNATDYRFILVQDGPVGVLNGEPKVDGKVANPSYILEEGQSGNLELKAAFRKDYSDVQLTLRASIVYYVAPNGKDIEKDNFYLEPHQDIITYVPVKSLSVKAGDNVITAKASMPENVFENYIQELYKKYGEDKVVIVRKRIEPIFITKKEYVVWEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8N1</accession>
    <name>A0A218P8N1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-haloalkanoic acid dehalogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854350.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8N1"/>
    <dbReference type="GeneID" id="33316019"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07080"/>
    <dbReference type="OrthoDB" id="115864at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050155">
        <property type="entry name" value="HAD-like_hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43434">
        <property type="entry name" value="PHOSPHOGLYCOLATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43434:SF1">
        <property type="entry name" value="PHOSPHOGLYCOLATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="224" mass="25866" checksum="4CCA984D7C038A15" modified="2017-09-27" version="1">MLRGLIFDVDETLVYYEGYNHRDWYEEWVKPELEKHGIALDYETYRRTVTGELPRSYVERFGIDHVEFWKLVDRVNLSYRQEMARLGRIKPFPDVSALKELRNLGLKLAAVSNASQDCTEFVLDLFNLRGYFDVVYGKDYSNLDGVKPNPYLVEKALKALNLKPEEALMVGDSAHDVLAGKGAGLRTVNVTRFGKVEGADYYVKDLWELVELVKELFSKRSPEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8S6</accession>
    <name>A0A218P8S6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">tRNA methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_07540</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854433.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8S6"/>
    <dbReference type="GeneID" id="33316111"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07540"/>
    <dbReference type="OrthoDB" id="14725at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031341">
        <property type="entry name" value="Methyltr_RsmF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011023">
        <property type="entry name" value="Nop2p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053630">
        <property type="entry name" value="tRNA_Cytosine-C(5)-MTase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00446">
        <property type="entry name" value="nop2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040814">
        <property type="entry name" value="tRNA_cyt_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF30">
        <property type="entry name" value="28S RRNA (CYTOSINE(4447)-C(5))-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17125">
        <property type="entry name" value="Methyltr_RsmF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="7">
        <location>
            <begin position="27"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="115"/>
            <end position="121"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="311" mass="35570" checksum="60AFF103E9C16B1B" modified="2017-09-27" version="1">MSAKDRIREVNPEFYERYSKLEDTDEFWEFIVKPLRQSIRVNTLKAPLEVVVERLREEFELEPIPWVREGFFINVDNLAKVPEHGLGLIFGQEASSMIPPVVLDPKPGELVLDMAAAPGSKTGQIAQYMENEGCIIANDPKLSRANVLIANLNRMGVLIARVTTRDGAYFARFEKAFDRILLDAPCSSVGMIRKKWRFLEEWRLKGVIKYMNIQKRLIRAAYDALKPGGTLVYSTCTIDPLENEEVVDYLLRKTDARLEKIDLPVKTSEPVLEWEGREYSPELKKALRIHPNDNDTEAFFIAKITKPEGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8S9</accession>
    <name>A0A218P8S9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_07510</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854427.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8S9"/>
    <dbReference type="GeneID" id="33316105"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07510"/>
    <dbReference type="OrthoDB" id="86099at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051258">
        <property type="entry name" value="Diverse_Substrate_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42920:SF5">
        <property type="entry name" value="EAMA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42920">
        <property type="entry name" value="OS03G0707200 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="231"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="6"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="149"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="280" mass="29895" checksum="42874DF11AF457A4" modified="2017-09-27" version="1">MRGESLGTLLALFGMLLYGFEPVVIKANPSNPVSFAAFSALFASLLLWMVLVFEGSGKELWDNPSGIKMAFLVGLFGTALAYLAYSFGARMSTAINAALITRSEVLWSFLLAGIFLGEKITRRLVAYSSLILAGLVLVITQGRAVEPHLGDVLLLLVPLFWQAGHVIAKRLPYRPITIATLRNTFGSLLLLPLALLTGLEFSPLVVAEGVIIALGQVVWYGSIKRINLSKATAIITPAPAVAIGIGILTGETLTAYHLLGFVLISIGTLGAVKVESELRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8U7</accession>
    <name>A0A218P8U7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Translation-associated GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ychF</name>
        <name evidence="5" type="ORF">A3L08_07780</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RelA/SpoT family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854473.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8U7"/>
    <dbReference type="GeneID" id="33316158"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07780"/>
    <dbReference type="OrthoDB" id="5875at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01669">
        <property type="entry name" value="TGS_MJ1332_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01899">
        <property type="entry name" value="Ygr210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000002">
        <property type="entry name" value="GTP pyrophosphokinase (RelA/SpoT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.470:FF:000001">
        <property type="entry name" value="GTP-binding protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013646">
        <property type="entry name" value="YGR210-like_G4"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007171">
        <property type="entry name" value="PRK09602.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF1">
        <property type="entry name" value="OBG-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08438">
        <property type="entry name" value="YGR210-like_G4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="4">
        <location>
            <begin position="1"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="397" mass="44238" checksum="05168C298D0D64D2" modified="2017-09-27" version="1">MEIGVVGKPNVGKSTFFSAATLVDVQIANYPFTTIDANVGVTYAIAEHPCKELGCTPNPQNYEYREGKSLIPIKMIDVAGLVPGAHEGRGLGNKFLDDLRMASALIHVVDATGKTDAEGQPTDHHDPVEDIEFLEREIDYWIYGILRKNWDKFSKRIKLQHLKLAQAIADQLTGIGVTEEDAFEAIHKLGLDNDPTKWSDEDLFNFVRELRKVNKPIIIAANKADAAADAQIERLIKKGRSRGYIVIPTSAAAELTLRKAAKAGFIDYVPGSSDFKILKKMSPKQEKALELIRERVLNRFGSTGVQEVINTATFELLNLVPVYPVEDEHKLTDQFGNVLPHVHLLPKGSTPRDLAYKVHTDLGKTFLYAVNARTHRRVGEDYELQFNDIIKIVATAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8U9</accession>
    <name>A0A218P8U9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Molybdenum cofactor biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07655</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854452.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8U9"/>
    <dbReference type="GeneID" id="33316134"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07655"/>
    <dbReference type="OrthoDB" id="205337at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00886">
        <property type="entry name" value="MogA_MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.980.10:FF:000006">
        <property type="entry name" value="Molybdenum cofactor biosynthesis protein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012245">
        <property type="entry name" value="MoaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43232">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43232:SF2">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006443">
        <property type="entry name" value="MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="1">
        <location>
            <begin position="17"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="171" mass="18341" checksum="E5F42D6533B0AF6D" modified="2017-09-27" version="1">MGAEEHKRKALRRFKFAVVTVSDTASRGEKEDKSGKFLVEELEKNGHEKVYYAVVPDEKMEIVGAVISAFRAGADVVVTSGGTGIAQRDVTIESIRPLFDKELTGFGEIFRLLSYEEIGTAAVMSRATAGIIKSSGRAMVVFCLPGSLGAAKTGIKIILNEAGHVLKHAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8V1</accession>
    <name>A0A218P8V1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein NO VEIN C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_07740</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854465.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="GeneID" id="33316150"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07740"/>
    <dbReference type="OrthoDB" id="359226at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052957">
        <property type="entry name" value="Auxin_embryo_med"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024975">
        <property type="entry name" value="NOV_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047352">
        <property type="entry name" value="P_loop_sacsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32387:SF0">
        <property type="entry name" value="PROTEIN NO VEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32387">
        <property type="entry name" value="WU:FJ29H11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13020">
        <property type="entry name" value="NOV_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein NO VEIN C-terminal" evidence="3">
        <location>
            <begin position="1466"/>
            <end position="1551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1340"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="695"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1298"/>
            <end position="1326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="1575" mass="186367" checksum="42861D928BE9BC7E" modified="2017-09-27" version="1">MGNTHSQANLTLKPKDIIRKIQEELFGIGIDSSELPENVKNYILRNNEFKRDAAKLAEDIHTKKPHFVFELIQNAEDNEYKEAVIPKIKFILTSDCLIVQNNETGFKEENVWALCKIGGSTKTNKSLGYIGEKGIGFKSVFMVANEVWIFSNGFQFRFEYNKNDPLTMLIPRWIEHVPDFVDPTQTNIILVLNDESKTKASRYLNDVHPNLLLFLRKLKIIEIEDRTRGIYKRFERFEKDNVVEIRIEEKADKLNKTSTLRWFVVKKTLTVPSEIFEDKRRGVKETEIILAFPLNEDGSPDASKEQHVFAFLPVRRYGFKFILQADFILPITREDIIRDSRWNAWLRDFIVDVFMDAVEEFKKDDKLKYCFYEYLPLDEVKDEFFLPVVEGIYRRLKEEECILTDANIWKKPSEVIVGNEEIKKIVPNEYLYQFLGKEYLSSKIKAKKQVLHKLGIRDFSIDDLIKILGNTEWLKKQGDEWFASLYRYLSNRKLSEEQLEDLRDLDIVRLENGELTSINKGTVFFPIEKERIYGFESELRIIRRDVIDIISKYGKEEGDKILEFLKELGIRHATPYEIIENYILPAYEGEDWKQKDSRTLIGYIRYIKDNMHKYERESDRRLNANKKSWETKEDPLKRLKKSLLIRINRDEGEEWYDNPENTYLPKIYGNENDLEELFEGIDVSFVHPCYLEQDIRHINNELSELENKLKDRSKTWKKKHKKEMKKIEKRIRDLKEEKEKRIREWKEFFLKLGVHEVPRVEYYEGSCGLSYSHTYCDFQSRFGIPKEEMKYSTREHRIRDWKLSPELEYILENGNERRMKILLTLLDKYWSEKYSKYRNMGYWWFYYLEYYRSFPSSFIRELKENIKLPTTQNVLASPPEVFLDKPEIRELLGDTVPYLAVEIKNNEFIEALGINTQANVGGVLNYLKALVRQESTDKEKFERLYEFLDKHFGEDATNIKKEFAENSLIFVPNSGKKYYSTEEVIWKDVGNIFGKNRIYLEKHYPKLKKFFVEKLGISEKPTPKDYANVLVDISKKEEISKEDKKIVLKIYEELNRSLNPDRVETLISQEDWWKDFIEEPIFLTDRNEFWYNDNDIFINDDNELYELFKDEENISFLWLPEEYPVDKIKFFIEACNLRYISKSTKVEPLLEESEYLKDQELTNRIRTLIPYVLRYLYWRENATYEKLKKEGIFEKIGEIKVYISETLKVKYSISISERNTVDKIAERKCIYYGGNLYVERENASLNDLAVEFSKVFGEVKGLDSFLLLIINIPPSKIEKIMEIQKIGKLPEGKAEFLRNVLKAEKIEEEKELEKSAEKTLSKEKRREGIHLSILTKTTPKLSTKPKEEENIIGSNEGIPDETGEKEWTPEFSSEEVPVRVEEYKLQKQSHIQEGHGKNSSMEEIHTLLSFRSTNSPPENLSEKTREEIGRWGEKYALRCIKEELVDRYPDASLVDTERGFRLERRGNVIVEVIWENKKGESGKPYDIRIIHKREGDKEEAFFVEVKTTPSMAKAIFHLSGNEWNFMKENGDKYCIYRVYGAGGSNPKVVKISNPIKLIYEGKIKVKNATVEIDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8W4</accession>
    <name>A0A218P8W4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_07835</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8W4"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07835"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="173" mass="19732" checksum="26BC6FA6A368CA45" modified="2017-09-27" version="1">MLALEFEIPMERFNQVMDAIVWNDASIEWVYYNEKRGSAVFRLSVPRECLEWFLLRLAMAAGPLEVTITETEHQKTYIHEAFLIHLNTVSGGYPVVVLLEYYRGVFYPTDITIVTLPNPPQELIDSVMNLTIGRVRLSTASHVGRSVSNNTLIMRLKTGVAERTYTEHLLTVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8X6</accession>
    <name>A0A218P8X6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ligand-binding protein SH3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854493.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8X6"/>
    <dbReference type="GeneID" id="33316182"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07885"/>
    <dbReference type="OrthoDB" id="116567at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009577">
        <property type="entry name" value="Sm_multidrug_ex"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36007">
        <property type="entry name" value="TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36007:SF2">
        <property type="entry name" value="TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06695">
        <property type="entry name" value="Sm_multidrug_ex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="153" mass="17198" checksum="C3301F83AC237651" modified="2017-09-27" version="1">MNEFLQVFLLSLIPTFEGRYAIVYSIGRGYSLWETLLAASLGVLFLSVILPAVLPYIDRVMLWLERTPLRKIAHLYLYYVERVRKKAHPYVEKWGFWGLFIFVAIPLPGTGVWTGALAAYILAVEENRAFLALLLGGLFSMAITLGPALGLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P907</accession>
    <name>A0A218P907_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF3783 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_07905</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854496.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P907"/>
    <dbReference type="GeneID" id="33316186"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07905"/>
    <dbReference type="OrthoDB" id="85763at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016621">
        <property type="entry name" value="UCP014543"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12646">
        <property type="entry name" value="DUF3783"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF014543">
        <property type="entry name" value="UCP014543"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="15351" checksum="365F138E24C78CB2" modified="2017-09-27" version="1">MGKVLLVGFSPDEAEAVRASLDGLGVFEVPEYCRDWVVSEIVAKAGELSGSSNWHLRKFVIMHGLSNEELKEVIRSVKALNPGRVIFATTTETALGWKLEDYLNELMEEDEYFQAMRWARREASQRKGQFLDIGKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P934</accession>
    <name>A0A218P934_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_08145</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854538.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P934"/>
    <dbReference type="GeneID" id="33316235"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08145"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044770">
        <property type="entry name" value="MFS_spinster-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505:SF79">
        <property type="entry name" value="PROTEIN SPINSTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505">
        <property type="entry name" value="SPINSTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="254"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="290"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="394"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="9"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="444" mass="49339" checksum="9F5A80066F5D9554" modified="2017-09-27" version="1">MRNLRRKVSIVLLVLMAAFLMADQNLLPPNYEEIMAEFGISETQMGLVSTIFVATSAIMTMLWGFLSDIGQRKKLLVIGVLLGEIPCFLTAYVNSYYQLLAMRLLTGIGIGSIIPIGYSLIADMFEEEKRGRGYSYIETAFGFGTLFGMIVAGMIASWRTPFIIAAVPNFILAPLFYIIAEEPKRGEGEKELREVLEAGYEYTYRLNWEAIKKSLQTKTNILIFLQGIIGTVPWGVIMYWLVSFLRVTRGMDKTTATFVLLIIGISSVVGSLLGGFVGDYFEARQRGGRAVITGLAIFIGMLAAIGIILYPLPSKLTLQHWVGIAIYSVLFVQFVSYAGPNVRAIVSQVNLPEDRGTVFGLFNILDNVGKATGPLFGGFLIEVLKGMGYSKALAYQYTLLIGALFWIPCALVWLWIRKSYPEDRDAVKEILRKRAEELKASKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P938</accession>
    <name>A0A218P938_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TrmB family transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_08215</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transcriptional regulator TrmB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854551.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P938"/>
    <dbReference type="GeneID" id="33316249"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08215"/>
    <dbReference type="OrthoDB" id="96194at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051797">
        <property type="entry name" value="TrmB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021586">
        <property type="entry name" value="Tscrpt_reg_TrmB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293:SF1">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11495">
        <property type="entry name" value="Regulator_TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159071">
        <property type="entry name" value="TrmB C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="5">
        <location>
            <begin position="8"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription regulator TrmB C-terminal" evidence="6">
        <location>
            <begin position="108"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ07305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="338" mass="38087" checksum="F49CD04169BB9130" modified="2017-09-27" version="1">MESLLEQLKKFGFTKYEALAYITLLTYGPLTARGISQRGQIPYNRTYDVLTSLKERGFVEELEGKARTFVAVEPEVAFHRYSRSLKELIDDIKKFAESMGVEEQKHVIWRFSSPEEVLLSLETMMKKAKFEFTLLAPKSFLPKVEDGLRELLEKGITVSIYTNGEPGLAAGGNVFIRLTDKIGHIIAMRDTTEVLVSPSLVFNVEEELPSGFKSNFPEIIFSYYIYLRDILEESKPVGFALNGNGEMRFAVFYHAIQAIEKFLTGGFDVDAEVHTTSGEVFNGKVVDFTNTKIINNFVLDTGERKALIGGPFSILEEYEAVGVVLYPKKSHRSEDVQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P958</accession>
    <name>A0A218P958_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CAAX protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854574.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P958"/>
    <dbReference type="GeneID" id="33316273"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08335"/>
    <dbReference type="OrthoDB" id="88777at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="231" mass="26419" checksum="BE12FD8D45B75645" modified="2017-09-27" version="1">MSEGRKTYDPTTWKPGRLDLILSVVITLSLPPLAGWLYDKKGALIPMLIYYGLAWGLVKLRRGVIGYRTPMPEKPPVWFYINVAVIIIALAFAYMSPIKVENPWLPGVVLTALLWAPANASSEQILWLYIFDSWDLYPRERRIGYRIVGLLLFAAFVGMIHAMFWTKFLQVARPDTVFGALFVLATAISGFLHIPVWRRSGQMVFTFLPHFLLNLGPLLWTGYSILPYLWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9A1</accession>
    <name>A0A218P9A1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0251 protein A3L08_08560</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_08560</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0251 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854614.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9A1"/>
    <dbReference type="GeneID" id="33316317"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08560"/>
    <dbReference type="OrthoDB" id="74471at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06171">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00674">
        <property type="entry name" value="UPF0251"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002852">
        <property type="entry name" value="UPF0251"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003257">
        <property type="entry name" value="PRK04217.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37478">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37478:SF2">
        <property type="entry name" value="UPF0251 PROTEIN TK0562"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02001">
        <property type="entry name" value="DUF134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="Sigma3 and sigma4 domains of RNA polymerase sigma factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="3">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="118" mass="13413" checksum="D2713894A76B54AB" modified="2017-09-27" version="1">MPRGMGQGWGRGRGRKRKMRMIGFIPQVRHFYPALPPLGQPKPPIFMTYEEFEALRLVDYEGLTQEEAAKRMGVSRGTVWRALSSARKKVAQMIVEGRELVILPQGNEVLKETTNDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9B3</accession>
    <name>A0A218P9B3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF998 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854610.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9B3"/>
    <dbReference type="GeneID" id="33316313"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08540"/>
    <dbReference type="OrthoDB" id="103507at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009339">
        <property type="entry name" value="DUF998"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42241">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED, DUF998 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42241:SF2">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED, DUF998 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06197">
        <property type="entry name" value="DUF998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="20849" checksum="0A5B3F70F8A83796" modified="2017-09-27" version="1">MRRSQFWAGLIAPLIAYSGILTAIYVNRSWWRLTDNAISDLGKVGLQHNWLLNVPLVLTAVLAIYYAAGLLEKARNKVEKIGIWVFIAGFVFLALIGIFPEGTSPHYYVSWGFFLTASLGLLIAGVGMGLAGEREMLYFTVALFVLAWILTIWAMRTFKGVAIPEFIGAIAITVWHYAILLKIKPDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9D9</accession>
    <name>A0A218P9D9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">23S rRNA (Uracil-5-)-methyltransferase RumA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_08780</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854651.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9D9"/>
    <dbReference type="GeneID" id="33316362"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08780"/>
    <dbReference type="OrthoDB" id="85343at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030390">
        <property type="entry name" value="MeTrfase_TrmA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030391">
        <property type="entry name" value="MeTrfase_TrmA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010280">
        <property type="entry name" value="U5_MeTrfase_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00479">
        <property type="entry name" value="rumA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11061">
        <property type="entry name" value="RNA M5U METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11061:SF30">
        <property type="entry name" value="TRNA (URACIL(54)-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05958">
        <property type="entry name" value="tRNA_U5-meth_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51687">
        <property type="entry name" value="SAM_MT_RNA_M5U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01230">
        <property type="entry name" value="TRMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01231">
        <property type="entry name" value="TRMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4 6" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="5">
        <location>
            <position position="377"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="377"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="422" mass="47164" checksum="FA5CCA5FB0C96824" modified="2017-09-27" version="1">MSLKGRVAGISDDGLGVLEVNGRRFYVPFAYPGDVVGVKEAKRRFGRKIARDFELVEGSPLRQGARCPHFGTCGGCLWQGLKYKEQLGLKAEIFERITGIDAEIRGSPRIWGFRNVSNFIVTVSGIGLKEYGNPLGVADLRECPVFSKRTPEYLHSLREFLNETRLKPWDLREKTGEVHYLQVREGKFTGEVMVNLIAHMKPLGGVIEAFKGYFSFADSLYWSLKTDTRDDPRGEAELIAGEPYIHERIEDVVYLIHPNSFFQTNSYALPLLLKAVEGFTDGEKVLDLYSGVGTFGVWLAKKGFKVEGVELNPFAVEMANKNAELNGVDATFRVGRAEETPIGDYDTVIVDPPRKGLKDAADLLVKGGVEKIVYVSCNPKAFRLDYKKHLKNAYRVENAVLIDMFPHTPHVEAVVELKKKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9E9</accession>
    <name>A0A218P9E9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Translation initiation factor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_08820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854659.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9E9"/>
    <dbReference type="GeneID" id="33316370"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08820"/>
    <dbReference type="OrthoDB" id="23364at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012041">
        <property type="entry name" value="Znf_CPxCG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42195">
        <property type="entry name" value="UCP015877 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42195:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19769">
        <property type="entry name" value="CPxCG_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015877">
        <property type="entry name" value="UCP015877"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="204" mass="23315" checksum="9E53A163CACCE6D5" modified="2017-09-27" version="1">MEEYFVCPECGSDDVEVIKERGRELTLKCNECGNVWHVTLPKFVKVPLIVSKHERSFKSEVELPEGEEIRVGDIVETEDDEVRITGIELEGDKRVEKARIGEIKTLWGESLTYPKVIKVSIYLPKGLTQAFKVKVPRDEEFVVGEVLEVGGYTFKVEKIKTERKMLHHGKAKADEIVAIMGHQIPRARAKRSLEIYRGYAKESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9F4</accession>
    <name>A0A218P9F4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854666.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9F4"/>
    <dbReference type="GeneID" id="33316378"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08860"/>
    <dbReference type="OrthoDB" id="130870at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030975">
        <property type="term" value="F:thiamine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015888">
        <property type="term" value="P:thiamine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13545">
        <property type="entry name" value="PBP2_TbpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027552">
        <property type="entry name" value="CGP_CTERM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026045">
        <property type="entry name" value="Ferric-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005948">
        <property type="entry name" value="ThiB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04288">
        <property type="entry name" value="CGP_CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01254">
        <property type="entry name" value="sfuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30006:SF2">
        <property type="entry name" value="ABC TRANSPORTER SUBSTRATE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30006">
        <property type="entry name" value="THIAMINE-BINDING PERIPLASMIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002825">
        <property type="entry name" value="CfbpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="379" mass="42768" checksum="19AD70078B9BC0D3" modified="2017-09-27" version="1">MRKLTTVMLVFLLMGPLGAIKPVTAEETLTVYSYDSIEWWMKEIIPLFEEKYGVKVNLVLIGDAGQVLNRLILEKDNPQADVVVGIDNSYLAKAIHAGVLEPYRPTNADVIPDWIVEKFDPTFHLTPYDYGYIAINYRKDMVQNPPKSLEDLTKPEWKGKLIIEDPRTSSPGMAFLLWTIAVYGDDWLEYWQKLKENDVQIVKGWSEAWGAFSEGEYPLVLSYATSPAATVYYENNTNVGAVAFEEGNYLQIEGAGVVKGARHPELAKKFIEFLISEEAQEKLPLNQWMYPVNKNVQIPDVFKYAVEVDKPVTVDPKDIEENYDTWLKQWTALMVEGKSPDEILGKTSTITESEGNGGICGPGIIVGLALLPVLLRRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9F8</accession>
    <name>A0A218P9F8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854669.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9F8"/>
    <dbReference type="GeneID" id="33316381"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08875"/>
    <dbReference type="OrthoDB" id="375249at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="134" mass="14743" checksum="7B2A3252DF0BA31B" modified="2017-09-27" version="1">MSRAWVYTREENSWKGKLTGLLIALLTAILAVLIVYPFTGGVSDGGLTGAVAGISAADLLSKTLKRLKKHAFKLYIGLVFLNTFASAFAVLRFGLHPENTAEFMVMFLAVYTPLALLDYGVIKIVEKRYRTKTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9I2</accession>
    <name>A0A218P9I2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1931 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_08905</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854674.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9I2"/>
    <dbReference type="GeneID" id="33316387"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08905"/>
    <dbReference type="OrthoDB" id="90610at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22922">
        <property type="entry name" value="HFD_Aq328-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22923">
        <property type="entry name" value="HFD_Aq328-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015207">
        <property type="entry name" value="DUF1931"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09123">
        <property type="entry name" value="DUF1931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="17558" checksum="A9E4D46CC720EDAF" modified="2017-09-27" version="1">MAEMIIPYPQLQKILERTCELAVIKPRAEEMMDIVEKKLADLFEVAYENAMAEGSETIKLRHIPITKGFKNSMNLFRAVIEDEKVQMEPIRKFVLKKIPGDIPLEEEVVNELPTIAGTLFVLVGRVIKALHPEIKNVYPEHIEEAEKVLDYTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9L9</accession>
    <name>A0A218P9L9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AMP phosphorylase</fullName>
            <shortName evidence="3">AMPpase</shortName>
            <ecNumber evidence="3">2.4.2.57</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Nucleoside monophosphate phosphorylase</fullName>
            <shortName evidence="3">NMP phosphorylase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_09185</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the conversion of AMP and phosphate to adenine and ribose 1,5-bisphosphate (R15P). Exhibits phosphorylase activity toward CMP and UMP in addition to AMP. Functions in an archaeal AMP degradation pathway, together with R15P isomerase and RubisCO.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>AMP + phosphate = alpha-D-ribose 1,5-bisphosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:36975"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:68688"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.4.2.57"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>CMP + phosphate = cytosine + alpha-D-ribose 1,5-bisphosphate</text>
            <dbReference type="Rhea" id="RHEA:36987"/>
            <dbReference type="ChEBI" id="CHEBI:16040"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:68688"/>
            <dbReference type="EC" id="2.4.2.57"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>UMP + phosphate = alpha-D-ribose 1,5-bisphosphate + uracil</text>
            <dbReference type="Rhea" id="RHEA:36991"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:68688"/>
            <dbReference type="EC" id="2.4.2.57"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.57" evidence="3"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854723.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9L9"/>
    <dbReference type="GeneID" id="33316443"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09185"/>
    <dbReference type="OrthoDB" id="9827at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004645">
        <property type="term" value="F:1,4-alpha-oligoglucan phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006196">
        <property type="term" value="P:AMP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046125">
        <property type="term" value="P:pyrimidine deoxyribonucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006206">
        <property type="term" value="P:pyrimidine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1170.30:FF:000004">
        <property type="entry name" value="AMP phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.970.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.30">
        <property type="entry name" value="Pyrimidine nucleoside phosphorylase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02132">
        <property type="entry name" value="AMP_phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017713">
        <property type="entry name" value="AMP_phosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036320">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036566">
        <property type="entry name" value="PYNP-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013102">
        <property type="entry name" value="PYNP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017872">
        <property type="entry name" value="Pyrmidine_PPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013466">
        <property type="entry name" value="Thymidine/AMP_Pase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000053">
        <property type="entry name" value="Thymidine/pyrmidine_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03327">
        <property type="entry name" value="AMP_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02645">
        <property type="entry name" value="ARCH_P_rylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003338">
        <property type="entry name" value="PRK04350.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10515">
        <property type="entry name" value="THYMIDINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10515:SF0">
        <property type="entry name" value="THYMIDINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07831">
        <property type="entry name" value="PYNP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000478">
        <property type="entry name" value="TP_PyNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00941">
        <property type="entry name" value="PYNP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47648">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54680">
        <property type="entry name" value="Pyrimidine nucleoside phosphorylase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00647">
        <property type="entry name" value="THYMID_PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyrimidine nucleoside phosphorylase C-terminal" evidence="4">
        <location>
            <begin position="424"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="256"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="194"/>
            <end position="199"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="503" mass="53880" checksum="AEE6B340471713AE" modified="2017-09-27" version="1">MKAKVRILDIYSGRYSVFINEKEAREAKLHPDDLVRLEAGKKTVYGSVVISSLIDEGEIGISRDILQLHNFSEGEVITVLPSGTPESVRYIKKKMNGEKLRKVEIEAIVRDIVDRKLRDIEISSFVTALEINGLDMDEIAALTIAMAETGDMLDIDRKPIMDVHSIGGVPGNKTNILVVPIVAAAGLTIPKTSSRAITSAAGTADVVEVFADVSFSLDEIKRIVEKVGACLVWGGALNLAPADDITIKAERALSIDPTGLMLASIMSKKYAMGSQYVLIDIPTGKGVKVESVDQARALARDFIELGKRLGQYVEVAITYGGQPIGHTVGPALEAREALSALMTGTGPGSLIEKATGLAGVLLEMGGVAPTGMGKKMAKEILESGKAWEKMKEIIEEQGGDPNIKPDDIPVGDKTYTFTASTSGYVTSIDNRAITGIARAAGAPEDKGAGIELYVKVGEKVKEGDPLFTIHAESEARLDQAIVFARRTEPIRIEGMVLQRIGNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9M5</accession>
    <name>A0A218P9M5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854729.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9M5"/>
    <dbReference type="GeneID" id="33316453"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09235"/>
    <dbReference type="OrthoDB" id="86175at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="88" mass="10451" checksum="C09CA318E0605AE5" modified="2017-09-27" version="1">MDERTSRLIEYTAEALLVSWLSYLFFYQNYLLYRWHRGLPLPSKTPFIIAGIIVGALLFLYEWFKFERELEKKHRTASESAAPDVSMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9N1</accession>
    <name>A0A218P9N1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ADP-specific phosphofructokinase</fullName>
            <ecNumber evidence="7">2.7.1.146</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">ADP-dependent phosphofructokinase</fullName>
            <shortName evidence="7">ADP-Pfk</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pfkC</name>
        <name evidence="8" type="ORF">A3L08_09265</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the phosphorylation of fructose 6-phosphate to fructose 1,6-bisphosphate using ADP as the phosphate donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>beta-D-fructose 6-phosphate + ADP = beta-D-fructose 1,6-bisphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20105"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.146"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Carbohydrate degradation; glycolysis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the carbohydrate kinase PfkC family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.146" evidence="7"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854735.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9N1"/>
    <dbReference type="GeneID" id="33316459"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09265"/>
    <dbReference type="OrthoDB" id="85200at2157"/>
    <dbReference type="UniPathway" id="UPA00109"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043844">
        <property type="term" value="F:ADP-specific phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008443">
        <property type="term" value="F:phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1110.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00561">
        <property type="entry name" value="ADP_PFKinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007666">
        <property type="entry name" value="ADP_PFK/GK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015990">
        <property type="entry name" value="ADP_PFK/GK_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011790">
        <property type="entry name" value="ADP_PFK_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02045">
        <property type="entry name" value="P_fruct_ADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21208">
        <property type="entry name" value="ADP-DEPENDENT GLUCOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21208:SF1">
        <property type="entry name" value="ADP-DEPENDENT GLUCOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04587">
        <property type="entry name" value="ADP_PFK_GK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015883">
        <property type="entry name" value="ADP-Pfk_glckin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51255">
        <property type="entry name" value="ADPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="4 7 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="441"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="441"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ07492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="461" mass="52647" checksum="58769EF65F012FD3" modified="2017-09-27" version="1">MVRELLEKARELSIYTAYNTNVDAIVYLNGEMVQRLIDEFGADAVKKRMDEYPREINEPLDFVARLVHALKTGKPMAVPLVNEELHTWFDSHFEYDVERMGGQAGIIANLLANLDFNRVLVYTPHLAKKQAMMFVDKPNLLYPVVEKGKLVLKHPHEAYRENDPIKVNRIFEFRAGTTFKLGNETVTVPFSGRFIVSARFESIRIYTEPELKPFLPEIGEMSDGAILSGYQGIKLRYSDGKDANHYLREAKKDILLLKREKDVKVHLEFASIQNRELRKKVIYNLFPLVDSVGMDEAEIAHVLNALGYSKLADRIFTYNRIEDTVLGGKILLDEMNLEVLQIHTIYYIMYITHADNPLSEDELRSSLELATTLAAARASLGEIRSPEDFKVGLNVPYNERGEYVKLRFEEAKRRLRTREYKVVIIPTRLVKNPVSTVGLGDTISAGAFTSYLALLKKKGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9Q9</accession>
    <name>A0A218P9Q9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2095 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09190</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854724.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9Q9"/>
    <dbReference type="GeneID" id="33316444"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09190"/>
    <dbReference type="OrthoDB" id="84782at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018662">
        <property type="entry name" value="DUF2095"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09868">
        <property type="entry name" value="DUF2095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="15774" checksum="DFCADB3310BBFB9A" modified="2017-09-27" version="1">MEEKKKKRPVDDFAWQEYDRQEFEETFPALARELKGEGIPVDAYRAKEEGEILKEEDEIMDFSGYSPTVIDFLRRCETDEEALEIINWMEERGEITHEMAKELRITLVEKGVRAFGPKKEWGWYERHRKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9S3</accession>
    <name>A0A218P9S3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Photosystem reaction center subunit H</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854766.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9S3"/>
    <dbReference type="GeneID" id="33316492"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09430"/>
    <dbReference type="OrthoDB" id="85079at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.240">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38137">
        <property type="entry name" value="PRC-BARREL DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38137:SF2">
        <property type="entry name" value="PRC-BARREL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC-barrel" evidence="1">
        <location>
            <begin position="4"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="102" mass="11069" checksum="E39F96538081EDD2" modified="2017-09-27" version="1">MVKIMASKLRDVELITDTGVRLGWVYDLSFDEETGDILVIVAEPDEDLDTSEFVTDHEGLLLVPVSAVKSIGEVIIIDSTKLAVKSKLRRPPTASALTGKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA07</accession>
    <name>A0A218PA07_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphopantetheine adenylyltransferase</fullName>
            <ecNumber evidence="7">2.7.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Dephospho-CoA pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Pantetheine-phosphate adenylyltransferase</fullName>
            <shortName evidence="7">PPAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">coaD</name>
        <name evidence="10" type="ORF">A3L08_01990</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(R)-4'-phosphopantetheine + ATP + H(+) = 3'-dephospho-CoA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19801"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="2.7.7.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the eukaryotic CoaD family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.3" evidence="7"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854862.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA07"/>
    <dbReference type="GeneID" id="33315003"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01990"/>
    <dbReference type="OrthoDB" id="53228at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004140">
        <property type="term" value="F:dephospho-CoA kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004595">
        <property type="term" value="F:pantetheine-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02164">
        <property type="entry name" value="PPAT_CoAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00647">
        <property type="entry name" value="PPAT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054937">
        <property type="entry name" value="CoaD_Thcocales"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023540">
        <property type="entry name" value="PPAT_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041124">
        <property type="entry name" value="CoaD_Thcocales"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001985">
        <property type="entry name" value="PRK00777.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695:SF46">
        <property type="entry name" value="BIFUNCTIONAL COENZYME A SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695">
        <property type="entry name" value="DEPHOSPHO-COA KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 7 10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="9">
        <location>
            <begin position="9"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="154"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ07619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="181" mass="20305" checksum="097B67C4EB17D991" modified="2017-09-27" version="1">MRKKYRKVVVGGTFDRLHLGHKALLRKAFEVGEYVYIGLTSDEMIRSKPYAEKILPYELRLRDLIKFFEVNGYSNYRVIKIHTSIGFADRMKSLEAIVVSEETYKGALVVNRAREENGLKPLDIVTIGLVKSSIGAKISSSLIRAGLIDPFGRPLSSGNETPRKTFKGKERNTSGDTHGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA16</accession>
    <name>A0A218PA16_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CopG family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854833.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA16"/>
    <dbReference type="GeneID" id="33316564"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09790"/>
    <dbReference type="OrthoDB" id="25654at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22231">
        <property type="entry name" value="RHH_NikR_HicB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="entry name" value="Met repressor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36215">
        <property type="entry name" value="BLL4998 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36215:SF1">
        <property type="entry name" value="BLL4998 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribbon-helix-helix protein CopG" evidence="1">
        <location>
            <begin position="6"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="94" mass="10926" checksum="1A28C6B9BC56FD80" modified="2017-09-27" version="1">MGTSVKITARIPPALVAEIDRLVNAGYYSNRSEVIKEALRDFLVRKRAIIEEAPDEEFIRLAKRAIEPILAEDWESEADEYWDKIKGVRYEQEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA28</accession>
    <name>A0A218PA28_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF835 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_02850</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA28"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02850"/>
    <dbReference type="OrthoDB" id="86314at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008553">
        <property type="entry name" value="DUF835"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33531:SF7">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33531">
        <property type="entry name" value="RUBRERYTHRIN SUBFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05763">
        <property type="entry name" value="DUF835"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="DUF835" evidence="2">
        <location>
            <begin position="125"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="254" mass="28807" checksum="F05BDBE4855F9389" modified="2017-09-27" version="1">MELTLKLGLEIVKLGIITLALFVFYRNRALFEVSLPQSLLRKFTYVTTIFWLGFLVDVINDVYPTSFTKLLDDIIICLALLLGTYYLIDHMRRARVAVEPSKVLKGTPSLEKGAYLADTTDIDSILKLLAGKRVIALTRTPGIFKERGIPYLWLSKVEGENSIDPRRLPAILHHLISTADENTAVIIDGVEYLILENGFENVLKFLTALKDHLLLKGALLIVVVSPEALDSRQFSHLRREFEELDVKRLKKESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA42</accession>
    <name>A0A218PA42_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2-oxoglutarate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_06760</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854909.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA42"/>
    <dbReference type="GeneID" id="33315955"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06760"/>
    <dbReference type="OrthoDB" id="30755at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03375">
        <property type="entry name" value="TPP_OGFOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051457">
        <property type="entry name" value="2-oxoacid:Fd_oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48084">
        <property type="entry name" value="2-OXOGLUTARATE OXIDOREDUCTASE SUBUNIT KORB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48084:SF1">
        <property type="entry name" value="2-OXOGLUTARATE SYNTHASE SUBUNIT KORB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="2">
        <location>
            <begin position="56"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="281" mass="31188" checksum="CB724C13C2363F48" modified="2017-09-27" version="1">MYLKPAYEIREKYLRKDMLPTIFCPGCGIGSALQYTLRAIDDLGLNQDEIVWVSGIGCSSRVPGFVNFDGLHTTHGRALAFATGIKLTNPDLKIIAFMGDGDAAAIGGNHFIHAIRRNLDVTVILINNFTYGMTGGQVAPTALKGLKGTTAPYGQFENPFDIADLAVSAGANYVARWSVFNYLQGINSIKKALQKEGFSLVEFLSPCPISFGRRNRMKTSPELLRWYQKITVPLAKAKKMPPEELEGKIVIGEFADRDRPGLVREYEAYKKRAKKMMGWEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA80</accession>
    <name>A0A218PA80_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_07645</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA80"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07645"/>
    <dbReference type="OrthoDB" id="102571at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="79" mass="9069" checksum="2C02F80AE102ACF5" modified="2017-09-27" version="1">MATEKENLAPSEEDEKKIKSVLPWGNPRFLSEVIEERAKEREEAIEIVEGLIDPDILPGEIKSIVKKPDKKELIEEIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-02-06" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>K7PBY4</accession>
    <name>K7PBY4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dCTP deaminase</fullName>
            <ecNumber evidence="3">3.5.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Deoxycytidine triphosphate deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">dut</name>
        <name evidence="3" type="synonym">dcd</name>
        <name evidence="5" type="ORF">A3L08_04840</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Enzyme Microb. Technol." volume="51" first="342" last="347">
            <title>Characterization and PCR applications of dUTPase from the hyperthermophilic euryarchaeon Thermococcus pacificus.</title>
            <authorList>
                <person name="Cho S.S."/>
                <person name="Sun Y."/>
                <person name="Yu M."/>
                <person name="Kwon S.H."/>
                <person name="Kwon S.T."/>
            </authorList>
            <dbReference type="PubMed" id="23040389"/>
            <dbReference type="DOI" id="10.1016/j.enzmictec.2012.08.001"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="journal article" date="2024" name="Protein Sci." volume="33" first="e5185" last="e5185">
            <title>dUTP pyrophosphatases from hyperthermophilic eubacterium and archaeon: Structural and functional examinations on the suitability for PCR application.</title>
            <authorList>
                <person name="Fukui K."/>
                <person name="Kondo N."/>
                <person name="Murakawa T."/>
                <person name="Baba S."/>
                <person name="Kumasaka T."/>
                <person name="Yano T."/>
            </authorList>
            <dbReference type="PubMed" id="39440877"/>
            <dbReference type="DOI" id="10.1002/pro.5185"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.36 ANGSTROMS) IN COMPLEX WITH DUMP</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the deamination of dCTP to dUTP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>dCTP + H2O + H(+) = dUTP + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:22680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
            <dbReference type="ChEBI" id="CHEBI:61555"/>
            <dbReference type="EC" id="3.5.4.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the dCTP deaminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.13" evidence="3"/>
    <dbReference type="EMBL" id="JQ744023">
        <property type="protein sequence ID" value="AFJ04533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853947.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="PDB" id="8Y1W">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.36 A"/>
        <property type="chains" value="A=1-155"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="K7PBY4"/>
    <dbReference type="SMR" id="K7PBY4"/>
    <dbReference type="GeneID" id="33315572"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04840"/>
    <dbReference type="OrthoDB" id="33242at2157"/>
    <dbReference type="UniPathway" id="UPA00610">
        <property type="reaction ID" value="UER00665"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008829">
        <property type="term" value="F:dCTP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006229">
        <property type="term" value="P:dUTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00146">
        <property type="entry name" value="dCTP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011962">
        <property type="entry name" value="dCTP_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02274">
        <property type="entry name" value="dCTP_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42680">
        <property type="entry name" value="DCTP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42680:SF3">
        <property type="entry name" value="DCTP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22769">
        <property type="entry name" value="DCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="dUTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="7" id="KW-0002">3D-structure</keyword>
    <keyword evidence="1 3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 3" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="79"/>
            <end position="84"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFJ04533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ06691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="8Y1W"/>
        </source>
    </evidence>
    <sequence length="155" mass="17692" checksum="BE8FA6398F625706" modified="2013-02-06" version="1">MMLPDWKIRKEILIEPFNEKSLQPAGYDLRVGKEAYINGRLINVEKEGKVVIPPKTHALILTLERVKLPDDVMGDMKLRSSLAREGLLGSFAWVDPGWDGNLTLMLFNASEEPVELNYGERFVQIAFIRLEGPAKNPYRGNYQGSQHLVLSKRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P4Z4</accession>
    <name>A0A218P4Z4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_00145</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853117.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P4Z4"/>
    <dbReference type="GeneID" id="33314633"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00145"/>
    <dbReference type="OrthoDB" id="85402at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="entry name" value="GAD-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029349">
        <property type="entry name" value="DUF4443"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054039">
        <property type="entry name" value="PH0730-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14544">
        <property type="entry name" value="DUF4443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22167">
        <property type="entry name" value="PH0730-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55261">
        <property type="entry name" value="GAD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH0730-like N-terminal" evidence="6">
        <location>
            <begin position="12"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4443" evidence="5">
        <location>
            <begin position="95"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF22167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ05854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="200" mass="22240" checksum="83CCBC6A61B623CB" modified="2017-09-27" version="1">MSWKRGAYPEFTLEDAVAVLFMLQEPVGRKTVSEVLELGEGSVRTLLRKLSQLGAISSTQRGHSLNENGQELVERIESCFSGAKPAGKIDGYPAYALVVRNPPEFKSIELRDEAIRFHAKGAMILVVRNGEIVFPEDGRPLRETMPEIAEELEERSKPEDGDMVVVTWAEKEADAMKSAYHVALTLKGECIPEEIISLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P4Z9</accession>
    <name>A0A218P4Z9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Orotidine 5'-phosphate decarboxylase</fullName>
            <ecNumber evidence="5">4.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">OMP decarboxylase</fullName>
            <shortName evidence="5">OMPDCase</shortName>
            <shortName evidence="5">OMPdecase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pyrF</name>
        <name evidence="10" type="ORF">A3L08_00150</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 8">
            <text>orotidine 5'-phosphate + H(+) = UMP + CO2</text>
            <dbReference type="Rhea" id="RHEA:11596"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="EC" id="4.1.1.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 5 8">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the OMP decarboxylase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.23" evidence="5"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853118.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P4Z9"/>
    <dbReference type="GeneID" id="33314634"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00150"/>
    <dbReference type="OrthoDB" id="94124at2157"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04725">
        <property type="entry name" value="OMP_decarboxylase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01200_A">
        <property type="entry name" value="OMPdecase_type1_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014732">
        <property type="entry name" value="OMPdecase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047595">
        <property type="entry name" value="OMPdecase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018089">
        <property type="entry name" value="OMPdecase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010386">
        <property type="entry name" value="PRK13813.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01740">
        <property type="entry name" value="pyrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32119">
        <property type="entry name" value="OROTIDINE 5'-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32119:SF2">
        <property type="entry name" value="OROTIDINE 5'-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00156">
        <property type="entry name" value="OMPDECASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 8" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="4 5 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 5 8" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orotidine 5'-phosphate decarboxylase" evidence="9">
        <location>
            <begin position="3"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="6">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="6">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="6">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="164"/>
            <end position="174"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR614732-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR614732-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ05855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="211" mass="23068" checksum="9465F704542D3120" modified="2017-09-27" version="1">MSRLILALDVYERERALEIAECTADYLWAIKVNWPLIIGSGLKIITELKQVTGLPIIADLKLADIPNTNRLIAKKVFEAGADYVIAHGFVGRDSVKAVMELGKTIIVVEMSHPGAKEFIQPVTDKLIEMANELGPFGVIAPATRPERVSYIRSKLKPGIKVITPGVGAQGGTASEVLKAGADYIIVGRAIYQSENPRESARRIFEEVEAWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P518</accession>
    <name>A0A218P518_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9 10">Cell division protein FtsZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ftsZ</name>
        <name evidence="13" type="ORF">A3L08_00175</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. Polymerizes to form a dynamic ring structure in a strictly GTP-dependent manner. Interacts directly with several other division proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="8">Assembles at midcell at the inner surface of the cytoplasmic membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 10">Belongs to the FtsZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853123.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P518"/>
    <dbReference type="GeneID" id="33314639"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00175"/>
    <dbReference type="OrthoDB" id="371908at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02201">
        <property type="entry name" value="FtsZ_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000023">
        <property type="entry name" value="Cell division protein FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00909">
        <property type="entry name" value="FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000158">
        <property type="entry name" value="Cell_div_FtsZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020805">
        <property type="entry name" value="Cell_div_FtsZ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045061">
        <property type="entry name" value="FtsZ/CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024757">
        <property type="entry name" value="FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00065">
        <property type="entry name" value="ftsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314:SF9">
        <property type="entry name" value="CELL DIVISION PROTEIN FTSZ 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314">
        <property type="entry name" value="CELL DIVISION PROTEIN FTSZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12327">
        <property type="entry name" value="FtsZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00423">
        <property type="entry name" value="CELLDVISFTSZ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01134">
        <property type="entry name" value="FTSZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01135">
        <property type="entry name" value="FTSZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 8 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 10" id="KW-0717">Septation</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="11">
        <location>
            <begin position="37"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="12">
        <location>
            <begin position="238"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="132"/>
            <end position="134"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="NCBIfam" id="TIGR00065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ05860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="417" mass="45135" checksum="9A27E76D29DCD569" modified="2017-09-27" version="1">MVFKLLEQAGIKLDLDDDAKTKNFEGFSDENLEDLIRIVIVGVGGSGNNTITRLFELGVQGAELIAMNTDAQHLARTKAHKKLLLGKEITQGKGSGGNPEVGYRAAEASAHEIAETIGDADLVFITAGMGNGTGTGAAPVVAKVIKERARHNGRFREPLVVSVVTYPFKNEGKVRIEKAKAGIKALMYYSDTVIIIENDKLLKLVPKLPINAAFRFADEIIARMVKGITETIKLPSMVNIDFADVYSVMHNGGAALIGIGESDSSNRAVDAVKNALENKMLEVEYGSGDKALVHFTVGPDVSIGEINDAMNIVYEKLGEKSEIKWGARIDEDMGKVVRAMVIMTGVKSPHILGGEHALQVGSSIKDNIITAEPVRPFKSANDDYDRIFNTISGRSEKKGKELPPYAKRVLDDFLDIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P531</accession>
    <name>A0A218P531_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cation:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_00365</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853159.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P531"/>
    <dbReference type="GeneID" id="33314677"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00365"/>
    <dbReference type="OrthoDB" id="85180at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002758">
        <property type="entry name" value="Cation_antiport_E"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006244">
        <property type="entry name" value="PRK08382.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34584">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER SUBUNIT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34584:SF1">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER SUBUNIT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01899">
        <property type="entry name" value="MNHE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019239">
        <property type="entry name" value="MrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ05896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="201" mass="23036" checksum="2031D12625B686AE" modified="2017-09-27" version="1">MSRVPFYLKERLERLRERVTHGQFEASKLPAWERIILTWAVLIAFWVVISGSLSLDNLLVGAAVTGIIAAFMRDLLTEDIRKSGHIAEKLLYFTFIYLPQYVVIMAFRLLESNLKVAKNVIFMDINPGIVKIKTDLHSDTGITILANSITLTPGTLTLDVNKKLGETYLYVHWIDVETLNREKAGEKIKGDIEEWLKKVFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P540</accession>
    <name>A0A218P540_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nickel import system ATP-binding protein NikD</fullName>
            <ecNumber evidence="10">7.2.2.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">A3L08_00285</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Ni(2+)(out) + ATP + H2O = Ni(2+)(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15557"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.11"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:15558"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">The complex is composed of two ATP-binding proteins (NikD and NikE), two transmembrane proteins (NikB and NikC) and a solute-binding protein (NikA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="7.2.2.11" evidence="10"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853143.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P540"/>
    <dbReference type="GeneID" id="33314661"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00285"/>
    <dbReference type="OrthoDB" id="18209at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015413">
        <property type="term" value="F:ABC-type nickel transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000016">
        <property type="entry name" value="Oligopeptide ABC transporter ATP-binding component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050388">
        <property type="entry name" value="ABC_Ni/Peptide_Import"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43297:SF13">
        <property type="entry name" value="NICKEL ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43297">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORT ATP-BINDING PROTEIN APPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="8"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ASJ05880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="326" mass="36413" checksum="8EE46FF36A6A999A" modified="2017-09-27" version="1">MPEPILEVRDLTVHFYTYAGIVKAIERVSFDVYRGETFALVGETGCGKSVTSRALTQLIESPGRIVEGSVIYHRDDGSTVDLLKLDPEEIRKIRGKEIAYIFQDPHASLDPLYTVGYQIAEAMVVHETVKDWKEGFRKAVDILRRVLIPDPENRVKNYPHEMSGGMKQRVVIGIGVSNNPKILIADEPTTALDVTVQAQILDLINKLKREYNTTVILITHNMGVVAEMADRVAVMYAGKIVEIGSVEQIFENPLHPYTQGLLRAVPNPLGKIDRLETIPGTVPNLIEPPSGCRFHPRCPRVMGACKEKVPELKEIEPGHFVACHLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P544</accession>
    <name>A0A218P544_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Indolepyruvate oxidoreductase subunit IorA</fullName>
            <shortName evidence="15">IOR</shortName>
            <ecNumber evidence="3 15">1.2.7.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 15">Indolepyruvate ferredoxin oxidoreductase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">A3L08_00465</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 15">Catalyzes the ferredoxin-dependent oxidative decarboxylation of arylpyruvates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>indole-3-pyruvate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = (indol-3-yl)acetyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12645"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17640"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57271"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="EC" id="1.2.7.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="15 16">Binds 2 [4Fe-4S] clusters. In this family the first cluster has a non-standard and varying [4Fe-4S] binding motif CX(2)CX(2)CX(4-5)CP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 15">Heterodimer of the IorA and IorB subunits.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.8" evidence="3 15"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853177.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P544"/>
    <dbReference type="GeneID" id="33314697"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00465"/>
    <dbReference type="OrthoDB" id="15347at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043805">
        <property type="term" value="F:indolepyruvate ferredoxin oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02008">
        <property type="entry name" value="TPP_IOR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000039">
        <property type="entry name" value="Indolepyruvate oxidoreductase subunit IorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000116">
        <property type="entry name" value="Indolepyruvate oxidoreductase subunit IorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045025">
        <property type="entry name" value="HACL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017721">
        <property type="entry name" value="IorA"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="583"/>
            <end position="612"/>
        </location>
    </feature>
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        <location>
            <begin position="614"/>
            <end position="643"/>
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        <location>
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        <location>
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            <name>[4Fe-4S] cluster</name>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        <source>
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        </source>
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        </source>
    </evidence>
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        </source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="646" mass="71239" checksum="4E540694BA6B43F8" modified="2017-09-27" version="1">MAKVTDMVLWDKPGEKVLLLGNQAIARGALEANIAVYAAYPGTPSSELTDTMAVVAKRAGVYMEYSTNEKVAFETALAAAWSGLRAMTAMKHVGLNVAADTFLSSVGMGVEGGFVIMVADDPSMWSSQNEQDTRVYAKFANVPVLEPSDPHEAKEMTKYAFELSEKFKHFVILRTTTRTSHARGDVILGELPEEIKQAKRKFGKFNKDPSRFVDVPSNARRFHPIVLEKIEKIREELNACPFNWIEGDENAKVGIIAPGLSYAYVKEALHWLGVENVKILKLGTPFPVPYGLLEKFMDGLEKVLIVEELEPVVEEQVKTWAYDRGLRIPIHGKDLVPRVYEMTTRRAVTAIAKFLGLETPLNYEKLDEKYKKALEIVPPRPPSLCPACPHRNSFFAIKKATHSKGIYPSDIGCYTLGLLPPLNAVDTTVAMGGSIGIAHGLEIAQHGSLSEEQRKKEKKIVVATIGDSTFYHTGLPALANAIYNRSNVLVVVLDNLVTAMTGDQPNPSTGQTPHGMGKRIPIEDVAKAMGADFVAVVDPYDIKATYETIKKALEVEGVSVVVSRQVCALYRIGQMRKRGEKWPIYYVDEEACTGCRLCVNAYGCPAIFWDEEKKQARVEPTMCWGCGGCAQICPFGAFKPVEEGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P569</accession>
    <name>A0A218P569_9EURY</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">A3L08_00625</name>
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        <name type="scientific">Thermococcus pacificus</name>
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            <taxon>Archaea</taxon>
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        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ASJ05944.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="59" mass="6732" checksum="5F5323FE5F036B5E" modified="2017-09-27" version="1">MGLSGAAWATLLVPTILGFLTMVLYGYWDRITGKEYYVDEEILAYDEDLVEELKKEGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P572</accession>
    <name>A0A218P572_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Apo-citrate lyase phosphoribosyl-dephospho-CoA transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_00585</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
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            <taxon>Thermococcales</taxon>
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            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
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        <property type="entry name" value="CITG FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42280:SF1">
        <property type="entry name" value="CITG FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01874">
        <property type="entry name" value="CitG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ05936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="308" mass="34883" checksum="DAF58A328095B2AA" modified="2017-09-27" version="1">MERFKIVRAFVLGPLLEAAIPKPGNVNRYRDFDDLTLYNFLFADTAVIGVYYEAIKTAELLRKGILGFNEAGIGELIKRAVQASREAQDANPNFGVITLSVPLIMGMVLGRNMLDAREKARLLLEESTVRDSMEFYRAIRIANPKGIPSGVKYDVYSDDSFRELFQDGINLARLAEMSCERELIFCEWLNGYELSYGTFGRLYELIKELPLEDAVVRAFLELLAEREDTLIARKAGREEAERVREKAREALEGRLSLDEFDAFMREKGDLRNPGSLADVMAVSLSLLVLRGLRMEIRNGKVWGVIGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5B8</accession>
    <name>A0A218P5B8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">CopY family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_00800</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BlaI transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853234.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5B8"/>
    <dbReference type="GeneID" id="33314764"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00800"/>
    <dbReference type="OrthoDB" id="84901at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005650">
        <property type="entry name" value="BlaI_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03965">
        <property type="entry name" value="Penicillinase_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ05972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="121" mass="14129" checksum="913A9E742B018EB7" modified="2017-09-27" version="1">MEPHEFKLTEEGIKAVLPPLEAEIMEHMWKVKVATAGQVYEYMKEKHPDIRRSTISILMNRLCERGLLKRSVDRGRGGMRYVYSITSTREEFEEKVVQSILDALMSNFKEATYAYLSKIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5C1</accession>
    <name>A0A218P5C1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ribose-phosphate pyrophosphokinase</fullName>
            <shortName evidence="11">RPPK</shortName>
            <ecNumber evidence="11">2.7.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">5-phospho-D-ribosyl alpha-1-diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Phosphoribosyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Phosphoribosyl pyrophosphate synthase</fullName>
            <shortName evidence="11">P-Rib-PP synthase</shortName>
            <shortName evidence="11">PRPP synthase</shortName>
            <shortName evidence="11">PRPPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">prs</name>
        <name evidence="13" type="ORF">A3L08_00930</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib-5-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 2 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 11">Belongs to the ribose-phosphate pyrophosphokinase family. Class III (archaeal) subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ05994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853256.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5C1"/>
    <dbReference type="GeneID" id="33314790"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00930"/>
    <dbReference type="OrthoDB" id="371997at2157"/>
    <dbReference type="UniPathway" id="UPA00087">
        <property type="reaction ID" value="UER00172"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000074">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00583_A">
        <property type="entry name" value="RibP_PPkinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037514">
        <property type="entry name" value="Rib-P_diPkinase_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002095">
        <property type="entry name" value="PRK00934.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF32">
        <property type="entry name" value="RIBOSE-PHOSPHATE PYROPHOSPHOKINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 11" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="12">
        <location>
            <begin position="7"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="32"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="89"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="213"/>
            <end position="217"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ05994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="279" mass="30549" checksum="B7CAA04F4CFF0376" modified="2017-09-27" version="1">MFVVGSGAKHLEDELKALGAGLIEAEIRKFPDGEKYVRVLGSADEVTVVQSTFRPQDERLVELILLADALRERGVETLKAVVPYFAYSRQDRVTKEGEPVSVRAIMRALAVYYDELYVFDLHNPETLKFFPSKAVNVSPARAIADYFREKLGDGIVLAPDKGARERAKAVAEKLGLEFSHFEKERISPTEVRMTPVDVDVRGKNVLIVDDIISTGGTMVKAANLLREMGAGKVFVAATHGVFAEGAIERVGRAVDELAVTNTIPTPVSRISIVPDILGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5G4</accession>
    <name>A0A218P5G4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aspartate carbamoyltransferase</fullName>
            <ecNumber evidence="7">2.1.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Aspartate transcarbamylase</fullName>
            <shortName evidence="7">ATCase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pyrB</name>
        <name evidence="11" type="ORF">A3L08_01080</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 7">Catalyzes the condensation of carbamoyl phosphate and aspartate to form carbamoyl aspartate and inorganic phosphate, the committed step in the de novo pyrimidine nucleotide biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterooligomer of catalytic and regulatory chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.2" evidence="7"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853285.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5G4"/>
    <dbReference type="GeneID" id="33314820"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01080"/>
    <dbReference type="OrthoDB" id="7792at2157"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00116"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004070">
        <property type="term" value="F:aspartate carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000001">
        <property type="entry name" value="Aspartate carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000021">
        <property type="entry name" value="Aspartate carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00001">
        <property type="entry name" value="Asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002082">
        <property type="entry name" value="Asp_carbamoyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00670">
        <property type="entry name" value="asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002032">
        <property type="entry name" value="PRK00856.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753:SF6">
        <property type="entry name" value="ASPARTATE CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753">
        <property type="entry name" value="ORNITHINE CARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00101">
        <property type="entry name" value="ATCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase carbamoyl-P binding" evidence="10">
        <location>
            <begin position="6"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase Asp/Orn-binding" evidence="9">
        <location>
            <begin position="154"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="309" mass="34686" checksum="45D7E170884F057D" modified="2017-09-27" version="1">MDWKGRDVISIRDFSKEDIEFVLRVAERLENELNEKGSLDYARGKILATLFFEPSTRTRLSFESAMHRLGGSVIGFSSAASTSVKKGESLADTIKTVEQYSDVIVIRHPMEGAARLAAEVADIPVINAGDGSNQHPTQTLLDLYTIKRAFGKIDGLTIGLLGDLKYGRTVHSLAEALAFYDVELYLISPELLRMPKHIVDELREKGVKVHETTDLEGTIPELDVLYVTRIQRERFPDEEEYLKVKGSYRVNCELLKSAKESMKIMHPLPRVDEIHPEVDKSAHALYFRQVFSGVPVRMALLGLTLGVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5P4</accession>
    <name>A0A218P5P4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF2110 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_01350</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853334.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5P4"/>
    <dbReference type="GeneID" id="33314875"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01350"/>
    <dbReference type="OrthoDB" id="120309at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056757">
        <property type="entry name" value="DUF2110_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056756">
        <property type="entry name" value="DUF2110_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056758">
        <property type="entry name" value="DUF2110_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016757">
        <property type="entry name" value="UCP019322"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09883">
        <property type="entry name" value="DUF2110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24873">
        <property type="entry name" value="DUF2110_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24872">
        <property type="entry name" value="DUF2110_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019322">
        <property type="entry name" value="UCP019322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2110" evidence="2">
        <location>
            <begin position="3"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2110" evidence="1">
        <location>
            <begin position="75"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2110" evidence="3">
        <location>
            <begin position="174"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="27283" checksum="AD9150CDA40D5632" modified="2017-09-27" version="1">MEEVVILEKVYGDRSGFLKLDRKLKAILGELEVEWKLSAVKKNWVKVSLKGEDEEISANLVREEFGEVPYSLKAVEEGKTYRGRFIDLGKVGYGAYVDIGIFRPRPKDALLPLYYLKETFGDIPVRQMIVRFGWVDNLPVEVIATKVEFGAREVELAFSKEQLKRIKSWLEDGHDKLFIAGTVSENVEKALIQTGHGRDVKRIEELGLMETLLVLKKGTQAPGIIKEIGPHLKGAVIGAIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5R2</accession>
    <name>A0A218P5R2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidase S9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_01665</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853388.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5R2"/>
    <dbReference type="GeneID" id="33314938"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01665"/>
    <dbReference type="OrthoDB" id="25019at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776:SF27">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE FAMILY MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776">
        <property type="entry name" value="SERINE PEPTIDASE S9 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="2">
        <location>
            <begin position="397"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="626" mass="72529" checksum="51F441D2A03C61C8" modified="2017-09-27" version="1">MSNIEWNEKTFSKFAYLGDPRIRSGFVAYTLTKANLDEDKYESTVVVENLETGAKRFVENASMPRISPDGKKLAFMRPNEEKKASEIWVADLQTLSAKKVLTAKNVRSVQWNDDSRRLLVVGFKRRDDEDFVFDDDVPVWFDSMGFFDGEKTTFWILDTESEEIIESFEKPRFSSGLWHGDSIVVNVPHREGSKPALFKFWDIYLWKDGEEEKLFERVSFEAVDSDGKSLLLRGKREKRFMSEHDWLYLWDGEPKPVYEGPLNTGQAKLDNGRVYFTLANAGRVNLHVWDGEVKPITVGDHWVMGFDVHEGKVVALIETATRLRELYIYDGELKQLTDYNGPIFKRLKTFEPRHFRFKSLDLEIDGWYLKPELNEDEKAPVIVFVHGGPKGMYGHYFKYEMQLMASKGYYVVFVNPRGSNGYDEDFALRVLERTGLEDFQDIMNGIEEFFKLEPQADRERVGITGISYGGFMTNWALTQSDLFKAGISENGISYWLTSHAFSDIGLWYDVEVIGPNPLENENYRKLSPLFYAGNVKAPILLIHSLEDYRCPLDQSLMFYHVLKDLGKEAYIAIFRRGAHGHSIRGKPKHRSKRYKLFFEFFERKLKKYEEGFDVEKILKGSPGPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5S2</accession>
    <name>A0A218P5S2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF91 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_01590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853375.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5S2"/>
    <dbReference type="GeneID" id="33314923"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01590"/>
    <dbReference type="OrthoDB" id="94838at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="318" mass="37063" checksum="4906D4789E648788" modified="2017-09-27" version="1">MSDYFLLSLSNRENLELCVKYNLAGFTNSINGLWTFFDIEVGDYISFLYGARVRNLYRVVKKVAYKNAEKLPPWSPITFRSKRTYYFPFRLFLKLEREFNEPMVRQEFSYVAENLLLRGGYRKTHFQADTITLYNVSEMGTPFTGESEHLELNAETFEPKIVFKRENQVIPEKSYFKELILQSLVRKKIKETVLGDALEFFGVDNSPSEFEVLGEKALPEGYVDIFIKPRHPSPKNKYLLAEVKTGKAQKKDVEQLRKYVKEFGDEAVGGILIAKDFSKSAFEDDKILPVRYHFEGLDTSLEYSYEELLKMLKLEVAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5U4</accession>
    <name>A0A218P5U4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ornithine aminotransferase</fullName>
            <ecNumber evidence="3">2.6.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_01845</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-ornithine + 2-oxoglutarate = L-glutamate 5-semialdehyde + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:25160"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="EC" id="2.6.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.13" evidence="3"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853421.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5U4"/>
    <dbReference type="GeneID" id="33314974"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01845"/>
    <dbReference type="OrthoDB" id="6534at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004587">
        <property type="term" value="F:ornithine aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000013">
        <property type="entry name" value="4-aminobutyrate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004426">
        <property type="entry name" value="PRK05769.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF58">
        <property type="entry name" value="LEUCINE_METHIONINE RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ06158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="445" mass="49631" checksum="D9A1144904094D03" modified="2017-09-27" version="1">MVVRPNVKELPGPKAKEVIKRNFEFLAYTTQDPETLPIVIDHGDGILVYDVDGNTFYDFGSGVGVLNVGHAHPRVVEAVKRQAEKFTHFALNDFFYENAVILAQKLAELAPGDFPKKVVYQNSGAEANEAMMKLVKYGTGRKRFIAFYHAFHGRTQAVLSLTASKWVQQDRFFPTMPGVEHIPYPNPYRNPWHIDGYAEPDELVNRVIEFIEEYVFRHVPPHEVGAIVFEPIQGEGGYVVPPKNFFKELKKLADSYGILLADDEVQMGVGRTGKFWAIEHFGVAPDTIQFGKAIGGGIPLAGVVHRADIAFDKPGRHASTFGGNPVAIAAGLEVVEIVKELLPHVQEVGDYLHKYLKEFEEKYEVIGDARGLGLAQAVEIVKSKDTKEKNPELRDRIVKEAVKRGLILLGCGDNSIRFIPPLTIQKEEIDVAMEIFEESLKAALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5W3</accession>
    <name>A0A218P5W3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">molybdopterin synthase</fullName>
            <ecNumber evidence="3">2.8.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">A3L08_01920</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Converts molybdopterin precursor Z into molybdopterin. This requires the incorporation of two sulfur atoms into precursor Z to generate a dithiolene group. The sulfur is provided by MoaD.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + cyclic pyranopterin phosphate + H2O = molybdopterin + 2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26333"/>
            <dbReference type="Rhea" id="RHEA-COMP:12202"/>
            <dbReference type="Rhea" id="RHEA-COMP:19907"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58698"/>
            <dbReference type="ChEBI" id="CHEBI:59648"/>
            <dbReference type="ChEBI" id="CHEBI:90778"/>
            <dbReference type="ChEBI" id="CHEBI:232372"/>
            <dbReference type="EC" id="2.8.1.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterotetramer of 2 MoaD subunits and 2 MoaE subunits. Also stable as homodimer. The enzyme changes between these two forms during catalysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MoaE family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.12" evidence="3"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853435.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5W3"/>
    <dbReference type="GeneID" id="33314989"/>
    <dbReference type="KEGG" id="tpaf:A3L08_01920"/>
    <dbReference type="OrthoDB" id="45235at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030366">
        <property type="term" value="F:molybdopterin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00756">
        <property type="entry name" value="MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1170.40:FF:000004">
        <property type="entry name" value="Molybdopterin biosynthesis protein MoeE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.40">
        <property type="entry name" value="Molybdopterin biosynthesis MoaE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036563">
        <property type="entry name" value="MoaE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003448">
        <property type="entry name" value="Mopterin_biosynth_MoaE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23404">
        <property type="entry name" value="MOLYBDOPTERIN SYNTHASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02391">
        <property type="entry name" value="MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54690">
        <property type="entry name" value="Molybdopterin synthase subunit MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ASJ06172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="142" mass="16596" checksum="A1103FDEEDA87BA8" modified="2017-09-27" version="1">MKVRLTKEPFDLNEALGHLLVPEAGAYVFFLGKVRNENHGRRVEKLIYEAYPEMAEAEMERIRKEALEKFPILDMLIWHRYGELRVGQDTILIIASGKHRREAFEACSWAIDEVKKRVPIWKKEVTDEGTFWIEGDRVVPEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P5Z4</accession>
    <name>A0A218P5Z4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853466.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P5Z4"/>
    <dbReference type="GeneID" id="33315022"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02085"/>
    <dbReference type="OrthoDB" id="102074at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="149" mass="16937" checksum="212AF50AD978D0E6" modified="2017-09-27" version="1">MFRRGEGRLGGAGISELRKSAELRTDGLFLWLPLLILFRLGFRFVTLIDPVLGMVFFYNAPLMLHLPRFFILSYPLIMGIAEVYFMVILRKGKLPFDLLFAYFMAAIVFEFPYSLLKSGDSGLILFALVWYATAPAGLPISLHSPWKIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P603</accession>
    <name>A0A218P603_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ORC1-type DNA replication protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">A3L08_02020</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in regulation of DNA replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the CDC6/cdc18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853453.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P603"/>
    <dbReference type="GeneID" id="33315009"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02020"/>
    <dbReference type="OrthoDB" id="195574at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08768">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18139">
        <property type="entry name" value="HLD_clamp_RarA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000073">
        <property type="entry name" value="ORC1-type DNA replication protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000930">
        <property type="entry name" value="ORC1-type DNA replication protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01407">
        <property type="entry name" value="ORC1_type_DNA_replic_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015163">
        <property type="entry name" value="Cdc6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055237">
        <property type="entry name" value="Cdc6_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014277">
        <property type="entry name" value="Orc1/Cdc6_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02928">
        <property type="entry name" value="orc1/cdc6 family replication initiation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001625">
        <property type="entry name" value="PRK00411.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF22">
        <property type="entry name" value="ORC1-TYPE DNA REPLICATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22703">
        <property type="entry name" value="Cdc6_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09079">
        <property type="entry name" value="WHD_Cdc6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01074">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="57"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc6 C-terminal" evidence="7">
        <location>
            <begin position="312"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="69"/>
            <end position="73"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="415" mass="47525" checksum="8AD55637DEB17A36" modified="2017-09-27" version="1">MDDNYLDSIFEKYLHAKKIFKNKEVLRHSYTPKELPHRREQIEELAHILVPVLRGETPSNVFVYGKTGTGKTVTIKFVTEELKKISHKYEIPVDVIYINCEIVDTQYRVLANIVNYFKHESGVEVPLVGWPTDEVYAKLKEVIDARERFVIIVLDEIDKLIKKSGDDILYSLTRINTELSLAKVSIIGISNDLKFKEYLDARVLSSLSEEEVVFPPYDANQLRDILMQRAKDAFNEGVLDEGVVPLCAALAAREHGDARRALDLLRVAGEIAEREGASKVTERHVWKAQEKIEQDTMEEVIKTLPLHSKVLLYAIVLLDENGELPANTGDVYSVYKSLCDHIDLEPLTQRRVSDLINELDMLGIINAKVVSKGRYGRTKEIRLNVTPYKVKNIYRHDPQLQTVLTISMSRQRRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P636</accession>
    <name>A0A218P636_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853499.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P636"/>
    <dbReference type="GeneID" id="33315059"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02270"/>
    <dbReference type="OrthoDB" id="65295at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027395">
        <property type="entry name" value="WH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37318">
        <property type="entry name" value="BSL7504 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37318:SF1">
        <property type="entry name" value="BSL7504 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13601">
        <property type="entry name" value="HTH_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="8"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="102" mass="11489" checksum="E81067ACF865AD46" modified="2017-09-27" version="1">MEAIRELTKNHTLGNPARLAIMLYLLPRERALFRDILEVLELTPGNLDSHLKALEKAGYIKVYKIIADRPRTAVKVTEKGAMETAKYMRALKEALSAIAPQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P652</accession>
    <name>A0A218P652_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TIGR00304 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02385</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853519.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P652"/>
    <dbReference type="GeneID" id="33315082"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02385"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002849">
        <property type="entry name" value="DUF131"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00304">
        <property type="entry name" value="TIGR00304 family membrane protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01998">
        <property type="entry name" value="DUF131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="82" mass="8209" checksum="3F4A7A271D41EC81" modified="2017-09-27" version="1">MDGKALILTGMALILIGFLLVFIGTLVSALGGESDVEGGGVIMIGPIPIVFGTNRGAAGAAMVLAIILMALWVIAALLSRGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P690</accession>
    <name>A0A218P690_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02545</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853549.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P690"/>
    <dbReference type="GeneID" id="33315115"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02545"/>
    <dbReference type="OrthoDB" id="26894at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022285">
        <property type="entry name" value="CHP03879_regulat_dom_put"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03879">
        <property type="entry name" value="near_KaiC_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40727:SF1">
        <property type="entry name" value="BACTERIO-OPSIN ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40727">
        <property type="entry name" value="TRANSCRIPTION REGULATOR, ENCODED NEXT TO RECA SUPERFAMILY ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="118"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="167" mass="19058" checksum="2CA1B1C4C7C6FDBA" modified="2017-09-27" version="1">MEIPLNPIGREEIHRLESILLFATLFRPEVIELIKDPAERLTWVDSLAVAAGAIAREKAGMTTGEIAEELGRTEQTIRKHLKGETKAGQLVRETYELIKQGKLDELVRNIEILAGGGKLVPEEECRKLEERVRELEEENRELKAKVENVRKILSDTLERVKEIEKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6B6</accession>
    <name>A0A218P6B6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0146 protein A3L08_02795</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_02795</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0146 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853597.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6B6"/>
    <dbReference type="GeneID" id="33315165"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02795"/>
    <dbReference type="OrthoDB" id="59816at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00341">
        <property type="entry name" value="UPF0146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005353">
        <property type="entry name" value="UPF0146"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003165">
        <property type="entry name" value="PRK04148.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03686">
        <property type="entry name" value="UPF0146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="134" mass="14800" checksum="F5E9CCFC10E74E65" modified="2017-09-27" version="1">MPVEDFAEFLAKEVPVGRVAELGIGFQFKVALKLKEFGYDVLAVDWNPVSVEKARELGLNAVRDDLFSPSLDLYLGVKALYSVRPTPEIVKPILELGEKLGVPVYILPLTGDTMPKALRLVNFRGLAIYTAKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6C1</accession>
    <name>A0A218P6C1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_02515</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853543.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6C1"/>
    <dbReference type="GeneID" id="33315109"/>
    <dbReference type="KEGG" id="tpaf:A3L08_02515"/>
    <dbReference type="OrthoDB" id="95481at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="191" mass="21627" checksum="B1FC01E16D0D32F5" modified="2017-09-27" version="1">MGKNGKPRLSYETSFRIKVGLLAVIFGIAIFMTVYYPLISHEVDPFRVSSPKGQILLAKNFSMMGVNYTNVVPFTAENNALVLGGKDEMEDTLTIKISTPGWCADLWIWGGSSSGWTRKYECLREFPLSKYAFNRVPTREAKEIVSWNLEKGYIIVFHKNSPVQNYELVNFTVTYGERTDWGAFKVAVKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6I3</accession>
    <name>A0A218P6I3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_157721579.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6I3"/>
    <dbReference type="GeneID" id="33315229"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03115"/>
    <dbReference type="OrthoDB" id="46222at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="1">
        <location>
            <begin position="6"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="69" mass="7925" checksum="C852C7E7F9F7814E" modified="2017-09-27" version="1">MELSKSPRNLGYGEGDDLGVRHTKGEYMVILDPKVEKNWLEEFLKPLEREEKLITNPKILTHDGSTLNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6I7</accession>
    <name>A0A218P6I7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thymidylate kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6I7"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03095"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thymidylate kinase-like" evidence="1">
        <location>
            <begin position="10"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="128" mass="15496" checksum="C3134330ACFA9CD4" modified="2017-09-27" version="1">MKVVLPRFIKKYDFIVLDRYIYDVRLALSKEFKTSFRYLRRLTKVLPSPDLIFFIEVSPEVAYQRKKEELESIEKTKKLFKNYQQLYFDVLREMGDKVIKVNNDGELMNSKIVILNELSKYWGDKNER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6M2</accession>
    <name>A0A218P6M2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein eS31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rps27ae</name>
        <name evidence="8" type="ORF">A3L08_03360</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eukaryotic ribosomal protein eS31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853694.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6M2"/>
    <dbReference type="GeneID" id="33315278"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03360"/>
    <dbReference type="OrthoDB" id="25142at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00777">
        <property type="entry name" value="Ribosomal_eS31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002906">
        <property type="entry name" value="Ribosomal_eS31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022845">
        <property type="entry name" value="Ribosomal_eS31_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001669">
        <property type="entry name" value="PRK00432.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01599">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01402">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Small ribosomal subunit protein eS31" evidence="7">
        <location>
            <begin position="13"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ASJ06436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="60" mass="7014" checksum="2B82E09E5A1D5F51" modified="2017-09-27" version="1">MTKGKGGKKTSQKWKMYEVQGGKVKRKNKFCPRCGPGVFMAEHKDRWSCGRCGYTEWKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6M5</accession>
    <name>A0A218P6M5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1102 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03410</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853703.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6M5"/>
    <dbReference type="GeneID" id="33315288"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03410"/>
    <dbReference type="OrthoDB" id="85476at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009482">
        <property type="entry name" value="DUF1102"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06510">
        <property type="entry name" value="DUF1102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="197" mass="21320" checksum="975E11295CBF34F9" modified="2017-09-27" version="1">MRKNIVLGIFGLLVAFGLVLGAGANFRDYNADRSVHWDIVSDDNELIDLTPVQPYAYINDGGVLVVDISPENPHWPGYGNGLSPNSEYNFDEVFNVSNDLWEEGMAIVVRITSDNSVIQFYGADMDIHDSANGTVVYASDNAKNDVCFVVQNGDAVSVGMDFTVGNDAPGTINNAAIHIEAYRLGTEPQELVGKCGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6N2</accession>
    <name>A0A218P6N2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03310</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853685.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6N2"/>
    <dbReference type="GeneID" id="33315268"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03310"/>
    <dbReference type="OrthoDB" id="85696at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="722" mass="81961" checksum="7F2EF27C46273BE7" modified="2017-09-27" version="1">MKRRAFIFTLDAILSVFLALIIITSIIGIEAQTRNVYSTYMRSSDKYLAEDTLTMLKTVPLSDIVPQEKLSEWISSGVINTTLVDPHMSPLEITTTYWSMDAKYPSLRLREKAGEILSFILDTALKDYNYELIISTNNSSNVYNYERPYIIKSGSNYNLTPDVMPATLILSGYEYNKTPRGYMARAYLSKLKAKETTYTYMGDYIATANDNTGPIKIRYIVPTSQFPSDARITGVKWLLEGAWTYSDYSIRINDKYVTCRDWDNGGWNNVHLNEELEDTNDNDYTTCNLVEEVQNSVDQNINVTFQVLVKHSMNSGEDGAQHIIITYKTSQASTFTYPHKFYFADVYANDSLDMEKYVFAPGNISELRIHIEGENIEVVTARFRIFNKLSDPITLSRSGNVFTADNATIASAIQSTGYTYQDISGSFFTIIFDITRASSSELHLDGTKSYVYVGYTPFVYASLYSIDFRKTLDDYTARNRKSGDFYNDVTWQWSLPEKGTPIYVKYQFPWLHDAGTVCDQDVRILSKDETTWITLYDAPQHNDFLDVFTRWGYTVTTRDYQGRVVEDAIHPGLNKFDLSFGKCYGVDPSHALGEVLFLLDAYAPYKDIKPKLLQGYPEVKAYNLTYKYIVGSTVRESSIIIGDKAAVSRGEYMNLTAAELKPGDYAVDDAVLRLFQKLGGDGWNHPILVELSDTKIDFASLGGIPSSISPIMVTLRVWRENS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6Q6</accession>
    <name>A0A218P6Q6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853715.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6Q6"/>
    <dbReference type="GeneID" id="33315303"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03490"/>
    <dbReference type="OrthoDB" id="56053at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052509">
        <property type="entry name" value="Metal_resp_DNA-bind_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33169">
        <property type="entry name" value="PADR-FAMILY TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33169:SF14">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR RV3488"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator PadR N-terminal" evidence="1">
        <location>
            <begin position="26"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="119" mass="13584" checksum="80AD35C6FEB7BF06" modified="2017-09-27" version="1">MLGNPKEKALKKLRKELRSGLYSYLILSILEREGELHGYAIRKRLGELSGGRLVPSEGALYDILKGLKKLGLVEDEWAEADGRPRKYYRLTELGREVLGELRDEIRETVMVVEKVNGGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6S8</accession>
    <name>A0A218P6S8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein A3L08_03700</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_03700</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853750.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6S8"/>
    <dbReference type="GeneID" id="33315345"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03700"/>
    <dbReference type="OrthoDB" id="25187at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.700.20">
        <property type="entry name" value="Hypothetical protein ph0010, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.150">
        <property type="entry name" value="Hypothetical protein ph0010, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00645">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023473">
        <property type="entry name" value="AMMECR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036071">
        <property type="entry name" value="AMMECR1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002733">
        <property type="entry name" value="AMMECR1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027485">
        <property type="entry name" value="AMMECR1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027623">
        <property type="entry name" value="AmmeMemoSam_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023472">
        <property type="entry name" value="Uncharacterised_MJ0810"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04335">
        <property type="entry name" value="AmmeMemoSam_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002000">
        <property type="entry name" value="PRK00801.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00296">
        <property type="entry name" value="TIGR00296 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016:SF0">
        <property type="entry name" value="AMME SYNDROME CANDIDATE GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016">
        <property type="entry name" value="AMMECR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01871">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143447">
        <property type="entry name" value="AMMECR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51112">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMMECR1" evidence="2">
        <location>
            <begin position="7"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="205" mass="23499" checksum="296EEF68D06C0CED" modified="2017-09-27" version="1">MYRIKDEWGEFLVRLARKAVEEYVRHGRTIKPPEDTPPELWEKMGVFVTLNRHDVPPQAALRGCIGFPLPIYPLVEATIKAAIYAAVDDPRFPPVRESELDDLVVEVSVLTPPEPVEGPPEERPKKIKVGRDGLLIEKGIYSGLLLPQVPIEWGWDEEEFLAQTCWKAGLPPDCWLDEDTKVYRFTAEVFEEEYPRGPVRRKPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6U2</accession>
    <name>A0A218P6U2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SAM-dependent tRNA/rRNA cytosine-C5 methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_03705</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853751.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6U2"/>
    <dbReference type="GeneID" id="33315346"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03705"/>
    <dbReference type="OrthoDB" id="14725at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1170:FF:000003">
        <property type="entry name" value="16S rRNA (Cytosine(967)-C(5))-methyltransferase RsmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.940.10">
        <property type="entry name" value="NusB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035926">
        <property type="entry name" value="NusB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006027">
        <property type="entry name" value="NusB_RsmB_TIM44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054728">
        <property type="entry name" value="RsmB-like_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF70">
        <property type="entry name" value="TRNA_RRNA CYTOSINE-C5-METHYLASE, NOL1_NOP2_SUN FAMILY, FUSED TO N-TERMINAL NUSB REGULATOR DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01029">
        <property type="entry name" value="NusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22458">
        <property type="entry name" value="RsmF-B_ferredox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48013">
        <property type="entry name" value="NusB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="6">
        <location>
            <begin position="179"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="389"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="269"/>
            <end position="275"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="451" mass="52527" checksum="22BCB28767222802" modified="2017-09-27" version="1">MELFYRVSFQEVVADALMLVEERELSSKHALERVFKRVVGKDREKARGLAHAYVFEIEKWRAKIDFIINSVLKGSTVEDLDPYLANLLRIGTFEIHFRKVPPAIATDSIIRVVKEKFDFSRAKFVNALMHSIEKFDVEKALKRLKEKDRIEWLSVRFSHPRWYVEYVIDLFGYDEAVRLLLSNNKPQRYYVRANTLKTDVDSLRDYLEENGVRTALTPVPDVLKVLEYKTPVTRLDWYKQGKFVIQDLASAYVAHVLNPDPGERVLDLAAAPGSKTFHAAALMENRGEIVAVDYSYDRLMRMKEKMKLLGIKNVKLVHADGQSFRDKDKFDKIILDAPCSSSGTYRQFPEVKWRFDGEKIKRIISVQRNMLRNAYENLRNGGEMTYSTCSIRIDEDEENVLFAVNRVGLELVPYEFSWGDRGFLEIGDKVFRAWTHRHDCNSFFIAKLRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P6U3</accession>
    <name>A0A218P6U3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA lyase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_03725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853755.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P6U3"/>
    <dbReference type="GeneID" id="33315350"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03725"/>
    <dbReference type="OrthoDB" id="275633at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="334" mass="38854" checksum="7F2970C8F9033A5E" modified="2017-09-27" version="1">MHLLPEQKPHETGSDFELSPKLIRENDTTPYQSSIVIDAERGAALAERLFQIYSNEGIFGKREMPEDYPPKGVKIGSREHLLFLTLTVAIDYMRNADALWESARKTFEDPETQFVFFPERLAEIPLREIENVKSALTKYKLALRPNRDTNIWLTIAMSLHEEWNDDPKNFLESMEFDALKIIEYLTKHRTKYPNLNGEKIRSLWLRMLRDNAGILLDNLEKVPIPVDVHITRATLSLGIIRGNFEGTLDEFREVVANAWMESTEQLRKSGKHIISLDLDRPLWHLSRNGCSYRGGTHPESCPKFEKCPFREYCTSGEIKIKRKGKHQYWVHLKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P701</accession>
    <name>A0A218P701_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Metal-dependent hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_03965</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853800.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P701"/>
    <dbReference type="GeneID" id="33315397"/>
    <dbReference type="KEGG" id="tpaf:A3L08_03965"/>
    <dbReference type="OrthoDB" id="52767at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011589">
        <property type="entry name" value="UCP004961"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42206:SF1">
        <property type="entry name" value="METAL-DEPENDENT HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42206">
        <property type="entry name" value="METAL-DEPENDENT HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004961">
        <property type="entry name" value="UCP004961_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="281" mass="31941" checksum="41DC11CCDC0D3659" modified="2017-09-27" version="1">MIIWDDHFHVDPYHGLFLEAVKEFHRAGGTHLVVVYKTAHDYGFPGLKAEDFIKAMDFHIELVEKINKETPVKAYAVVGVHPAEFDYLAREKGLEYAKSEVMKALEYAQKLCLEGKAIGIGEIGRPHYEVPPEIWEASIELMKYGMALAKEADCAVQLHTESFDEAKFRELGEYVKEVGIKPYKVVKHFSPPLVKVAEEVGVFPSIISSRKNIKAAIEQGNRFMMETDYIDDKRRPGAVLGPKTVPKRTKAFLQNGIFTEEDVYNIHVENPRKVYGVEVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P739</accession>
    <name>A0A218P739_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Fe-S-cluster oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_04325</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853861.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P739"/>
    <dbReference type="GeneID" id="33315469"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04325"/>
    <dbReference type="OrthoDB" id="36424at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005358">
        <property type="entry name" value="Puta_zinc/iron-chelating_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35866">
        <property type="entry name" value="PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35866:SF2">
        <property type="entry name" value="YKGJ FAMILY CYSTEINE CLUSTER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03692">
        <property type="entry name" value="CxxCxxCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="166" mass="19462" checksum="57C662E5577EB06A" modified="2017-09-27" version="1">MERRWVATIDLETLEVEYDPTFKFKCLENCGRCCHELEIPVRDEDITEIEGLGYNAWEFVDYDKMFYRGDKFLSYALKKRPFDGACVFLDPETKRCKIYPHRPLACRLYPFVFVKSGKKMEVYVKMDPFCPGLNHPDGEPVTGEFLLRDYGDVIGEYRGKIVKATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P765</accession>
    <name>A0A218P765_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein eL15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl15e</name>
        <name evidence="7" type="ORF">A3L08_04455</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853881.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P765"/>
    <dbReference type="GeneID" id="33315495"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04455"/>
    <dbReference type="OrthoDB" id="8183at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1120.10:FF:000002">
        <property type="entry name" value="50S ribosomal protein L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1120.10">
        <property type="entry name" value="Ribosomal protein l15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00256">
        <property type="entry name" value="Ribosomal_eL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024794">
        <property type="entry name" value="Rbsml_eL15_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000439">
        <property type="entry name" value="Ribosomal_eL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020926">
        <property type="entry name" value="Ribosomal_eL15_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020925">
        <property type="entry name" value="Ribosomal_eL15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003269">
        <property type="entry name" value="PRK04243.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847">
        <property type="entry name" value="RIBOSOMAL PROTEIN L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00827">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01384">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01194">
        <property type="entry name" value="RIBOSOMAL_L15E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="163"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="194" mass="22561" checksum="AA66AE7600E95F9E" modified="2017-09-27" version="1">MSMYKYIREAWKSPKKSYVGDLLKARMIKWRREPVVVRAERPTRLDRARSLGYQAKQGYVIVRVRVRKGGRKRPRWKGGRKPSKMGMVKYSPKKSLQWIAEEKAARKYPNLEVLNSYWVGEDGMYKWFEVIMVDPHHPVIKADPKINWITGKAHKGRVFRGLTSAGRKSRGLRNKGKGAEKVRPSIRANKGRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P794</accession>
    <name>A0A218P794_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TIGR00341 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_04480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853886.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P794"/>
    <dbReference type="GeneID" id="33315500"/>
    <dbReference type="KEGG" id="tpaf:A3L08_04480"/>
    <dbReference type="OrthoDB" id="3266at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005240">
        <property type="entry name" value="DUF389"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00341">
        <property type="entry name" value="TIGR00341 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20992">
        <property type="entry name" value="AT15442P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20992:SF9">
        <property type="entry name" value="AT15442P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04087">
        <property type="entry name" value="DUF389"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="316"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="36204" checksum="39463D9900CF3D81" modified="2017-09-27" version="1">MLRLEVNCDEGEGEKVEEVLRKWNVQYYTEEVRSNGKRVLRFTALVPEFVINDIADDLMKAIDLRKGYSSITWASVSGKSVKYSSSVKSLAKYKRRWTLVDIEGLIENANNQARVDPIQLTLGAVASIIALFGLIQDSIVMIISAMLLSPILGPLYGFSINVVMGKGRDAVSAVYSILKLLGVIFASALIVSLLLEIFGAMPGEPTHEILIRGQSGIVYILLAIILGYAGIVAIVSRVPEILAGVSIAAALVPPTTVIGISLAMGWWEIFEGSLVLVLENVLGLLSGSLLGLYVLNVSPRSYYEKRAAKIYTKRTMAVLALMVFALVLLELVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7H6</accession>
    <name>A0A218P7H6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF340 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088853991.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7H6"/>
    <dbReference type="GeneID" id="33315628"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05120"/>
    <dbReference type="OrthoDB" id="86270at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="8572" checksum="F77435FBF6452EAB" modified="2017-09-27" version="1">MNIFIPLLAGVLIGYTLRKRGKKLNVDLPMSAALLLLIFFMGVEAGKVEINAGWLLVSSIVFAALTIAGSVGMALLVGGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7N3</accession>
    <name>A0A218P7N3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hydrogenase expression/formation protein HypE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_05355</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HypE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854035.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7N3"/>
    <dbReference type="GeneID" id="33315675"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05355"/>
    <dbReference type="OrthoDB" id="31494at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02197">
        <property type="entry name" value="HypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011854">
        <property type="entry name" value="HypE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02124">
        <property type="entry name" value="hypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30303:SF0">
        <property type="entry name" value="CARBAMOYL DEHYDRATASE HYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30303">
        <property type="entry name" value="HYDROGENASE ISOENZYMES FORMATION PROTEIN HYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005644">
        <property type="entry name" value="Hdrgns_mtr_HypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="2">
        <location>
            <begin position="42"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="3">
        <location>
            <begin position="162"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="338" mass="35856" checksum="2B09C1E098D10AF2" modified="2017-09-27" version="1">MGEKIKLEHGAGGEIMEELLRDVILKTLTLKSAGGIGLDALDDGATIPFGDKHVVFTIDGHTVKPLFFPGGDIGRLAVSGTVNDLAVMGAQPLALANSMIIGEGLDMDVLERVLRSMDETAREVPVPIVTGDTKVVEDPIEMFVITAGIGIAERPISDAGAKVGDAVLVSGTIGDHGIALMSHREGIAFETELKSDVAPIWDVVKAVGDAIGWENIHAMKDPTRAGLSNALNEIARKSNVGILVREADIPIRPEVRAASEMLGISPYDVANEGKVVMVVAKEYAEEALEAMRKTEKGKNAAIIGEVIDQYRGKVILETGIGGKRFMEPPEGDPVPRIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7P5</accession>
    <name>A0A218P7P5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Energy-coupling factor ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_05520</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Probably part of an ABC transporter complex. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854066.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7P5"/>
    <dbReference type="GeneID" id="33315708"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05520"/>
    <dbReference type="OrthoDB" id="18209at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000224">
        <property type="entry name" value="Energy-coupling factor transporter ATP-binding protein EcfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050095">
        <property type="entry name" value="ECF_ABC_transporter_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43553:SF21">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN MA_1418-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43553">
        <property type="entry name" value="HEAVY METAL TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="4"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="286" mass="31891" checksum="92126FA09023974C" modified="2017-09-27" version="1">MNVITVEDLSFKYRRAERYSLRDVSFTVKEGELLGIIGPSGSGKSTLCLTLNGVIPNSIRGEFKGDVIIRDPRTGEEFNTKETPVARLSTVVGLVLQNPESQLFNMTVEEEIAFGLENLGLERNEILRRLRWALEVTGLKGLEKEFPPNLSGGQQQRLAIAAILAMGPSVLVLDEPTSQLDPVGRKEVLGLVSLLNREHGMTVVLVEHHTDYILRYADRVMVMTDGSIVMEGKPEEIAEEAEELRRLGIKLPAVLEVSHELRKRELLDRVVLTPEELLSTLKCPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7R2</accession>
    <name>A0A218P7R2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Undecaprenyl-diphosphatase</fullName>
            <ecNumber evidence="3">3.6.1.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Undecaprenyl pyrophosphate phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">A3L08_05485</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 15">
            <text>di-trans,octa-cis-undecaprenyl diphosphate + H2O = di-trans,octa-cis-undecaprenyl phosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28094"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60392"/>
            <dbReference type="EC" id="3.6.1.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UppP family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.27" evidence="3"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854060.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7R2"/>
    <dbReference type="GeneID" id="33315701"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05485"/>
    <dbReference type="OrthoDB" id="65864at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050380">
        <property type="term" value="F:undecaprenyl-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003824">
        <property type="entry name" value="UppP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30622">
        <property type="entry name" value="UNDECAPRENYL-DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30622:SF2">
        <property type="entry name" value="UNDECAPRENYL-DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02673">
        <property type="entry name" value="BacA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="253"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ASJ06809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="271" mass="28733" checksum="31D846717E8840B2" modified="2017-09-27" version="1">MVASLDYISPLISGIIVALTSWFPIGSGGYAVSRLLEAVAPVYGDYLVPSYMGVIFAVLFYFKDLIGPDTQKALVGRFSSTIKYFVYAAIFTVLVGYPLARGLGGSLGPRTSDLVNAVIGAGLVVVGVIYWKHLRAPLEDVEERVREDEEDTTLIDALISGVAQGVALIGGISNVGLVLLGLSSTGINIKKALELTFVVAPIYLTMKLLFMGGGSPELPVSLLFTAFVASFITSILTMRALLKAAEALGRETFLVLFGSVSIIVCLMGVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7T5</accession>
    <name>A0A218P7T5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05695</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854098.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7T5"/>
    <dbReference type="GeneID" id="33315743"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05695"/>
    <dbReference type="OrthoDB" id="94237at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="197" mass="22210" checksum="1FB01193C4BB1FEC" modified="2017-09-27" version="1">MSLVDAFQPVIVFYYAPKDIPLERVKEVFPSALEFKNPDVASFLTLSEGISGRTSLLILDVKDGSRYVFIQPLEKRIIAVNEMIPKVYVFHPKQAGEFFSLLAKDELDKITESKLRKGNPWRFLLDGILAIVIAALSDYFNLGLWGAIILGALFSIVEYLFGPKGQKGKLINVMPSKTWSQMLKKAEKKGELERITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7T8</accession>
    <name>A0A218P7T8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PH1570-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05615</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854083.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7T8"/>
    <dbReference type="GeneID" id="33315727"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05615"/>
    <dbReference type="OrthoDB" id="85738at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1600.10">
        <property type="entry name" value="PH1570-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023110">
        <property type="entry name" value="PH1570-like_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036807">
        <property type="entry name" value="PH1570-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09638">
        <property type="entry name" value="Ph1570"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159902">
        <property type="entry name" value="PH1570-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH1570-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="159" mass="18658" checksum="094709FF61B3541F" modified="2017-09-27" version="1">MLCEEKLEVFENGFEDGKFNLRIEFYGKDARRLLLAIIRELYLQDYGEDYVYPFECAKEFWGIYMDAGEIRAEDFRPSPVKFMNQSILNRLEKALETIDAPKDVRESIDFEKVEVHKLKKGLLAMGKNFILREDGYLFIFNKPSARGLILKYMGMLDGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7X4</accession>
    <name>A0A218P7X4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sodium:calcium antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">A3L08_05885</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854136.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7X4"/>
    <dbReference type="GeneID" id="33315781"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05885"/>
    <dbReference type="OrthoDB" id="142185at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008273">
        <property type="term" value="F:calcium, potassium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004481">
        <property type="entry name" value="K/Na/Ca-exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846:SF8">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YRBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846">
        <property type="entry name" value="SODIUM/POTASSIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="260"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="289"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="6">
        <location>
            <begin position="10"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="6">
        <location>
            <begin position="168"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASJ06886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="310" mass="32665" checksum="4031CF3966E75FF7" modified="2017-09-27" version="1">MLEFALWSLSILAGIAILVVVGDKLSDKIVEVARKAGISTLVISIVLVSLSTTLPEITTSAIASYQGANGIALGNALGSIFANIALILGLASMIRPLKAGRSAYENSLIMLASLVFLILLSIDGTLSRLDGLLLLLAYAVYLRWLLKKHARSEVEWEPSGDVKVLDYIILIALGLFLVGGAEMVVFGGKNIARALGISEFVIGATVVAIGTSLPEMTNALYGAVRERGSISVGNIIGANIMNALVVLGIASLIRPLPTDASVLTVVLVLFAMIPMIASLRKAGGIDRRVGAYFLALYVLYLVLIFSGVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7X6</accession>
    <name>A0A218P7X6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Polysaccharide deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_05770</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854113.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7X6"/>
    <dbReference type="GeneID" id="33315758"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05770"/>
    <dbReference type="OrthoDB" id="10436at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10941">
        <property type="entry name" value="CE4_PuuE_HpPgdA_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045235">
        <property type="entry name" value="PuuE_HpPgdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47561">
        <property type="entry name" value="POLYSACCHARIDE DEACETYLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G05030)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47561:SF1">
        <property type="entry name" value="POLYSACCHARIDE DEACETYLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G05030)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NodB homology" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="240" mass="28366" checksum="350EA0A0F1110E64" modified="2017-09-27" version="1">MMVSITFDVEHDCPPYLTTTRGMEKGLPKLLDLMAEKRVRATFFFTAEMAKRFPQLVKRVIDEGHELGSHNYNHERLDKLSRDEGRKAIEKSLEVLRGFDDVVSFRAPNLQFPNYYYDVLEKNGILVDSSKATYKGYREGVKFFGNVLEVPASTTSSVIRLPWRFQRIIHSRLKEPRVYFAHPWEFVPMQKEKIRWDCRFNTGDKAIELLGMIIDHYRSQGARFFTMREYYDLYGKLKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Y5</accession>
    <name>A0A218P7Y5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Probable cobalamin biosynthesis protein CobD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">cobD</name>
        <name evidence="12" type="ORF">A3L08_05900</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cell membrane</location>
            <topology evidence="2 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the CobD/CbiB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854139.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Y5"/>
    <dbReference type="GeneID" id="33315784"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05900"/>
    <dbReference type="OrthoDB" id="46105at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048472">
        <property type="term" value="F:threonine-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00024">
        <property type="entry name" value="CobD_CbiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004485">
        <property type="entry name" value="Cobalamin_biosynth_CobD/CbiB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00380">
        <property type="entry name" value="cobal_cbiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34308">
        <property type="entry name" value="COBALAMIN BIOSYNTHESIS PROTEIN CBIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34308:SF1">
        <property type="entry name" value="COBALAMIN BIOSYNTHESIS PROTEIN CBIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03186">
        <property type="entry name" value="CobD_Cbib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="48"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="294" mass="33142" checksum="E7692F81AAD47BA3" modified="2017-09-27" version="1">MDALIVFALALLWDLLLGEPPVLAHPVVWFGKLAGLIDFRYKRRSPALDFLAGTVTGLLVVAFAFLLSILPFFVPYPLNYLLAVYFLKSSFAVRSLHEHVARTITGDVEEKRKAVSMIVSRDVSKLDEPHLNSAAIESLAENLNDSVVAPLFYFILFGLPGALVYRAINTLDAMLGYRNERYEYFGKFSARLDDVLNFIPARLTVLLYLPLGGRNVFRYYRLAKFKINSDKPIAVMSAVLGVWLEKPGVYRFPGRAPRDKDIKRALRVYWIVVAEWVVILSLLLATEVCPCLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Y8</accession>
    <name>A0A218P7Y8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05870</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854133.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Y8"/>
    <dbReference type="GeneID" id="33315778"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05870"/>
    <dbReference type="OrthoDB" id="98055at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021578">
        <property type="entry name" value="STK_08120-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11485">
        <property type="entry name" value="STK_08120-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="228" mass="25714" checksum="5CB28EF12244064B" modified="2017-09-27" version="1">MKTRSWEVEVSADWETLRVILSDPKKTLPFFPYFGSIEGDVVRFKVPRFVFNFGYEFEFDVGFGEREAIYTFRGERGILTVTFKMIREKLRVTASWAGFGEALMGKPLENFAKGIAEAIKEFCSSMKCPGVKIEGESGEVEHITPETAPAFLKRLVVELGTDFVIEGRAEDGTYLSARIEGGRLKSLKVRTDKGESVIEADVPVVELGSELFEGLPLEKEFKIMVRKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Z5</accession>
    <name>A0A218P7Z5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cobalamin biosynthesis protein CobY</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_05925</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854144.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Z5"/>
    <dbReference type="GeneID" id="33315789"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05925"/>
    <dbReference type="OrthoDB" id="9782at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19136:SF86">
        <property type="entry name" value="ADENOSYLCOBINAMIDE-PHOSPHATE GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19136">
        <property type="entry name" value="MOLYBDENUM COFACTOR GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MobA-like NTP transferase" evidence="2">
        <location>
            <begin position="3"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ06894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="179" mass="19599" checksum="6DFDB4A8E41A46BF" modified="2017-09-27" version="1">MIIVMAGGRSTRMGKEKPVLRVGGKPMLLRVYGEAEKAGETLVTLSRNTPKTKEWCLRERIPFVETPGRGYVEDVRWLLREFGPFVSVSSDLPFVRASDIGSIAKAFDGKTSLTGVLPLERVPGDLKPVVYRGYAVVGLNAVGAEGENFFELSNPLLALNVNTPKDLKLAERIAKLVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P7Z9</accession>
    <name>A0A218P7Z9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_05970</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854153.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P7Z9"/>
    <dbReference type="GeneID" id="33315798"/>
    <dbReference type="KEGG" id="tpaf:A3L08_05970"/>
    <dbReference type="OrthoDB" id="88510at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ06902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="126" mass="14016" checksum="93E2B20C723020EA" modified="2017-09-27" version="1">MNSETNVPDDYFYVLGRNGTVRKFDLGRGFLSLRNAFLVSNGSYVLTGFERPQPDGSPMSGYVMILNGTKVVWSRTFQINDPSCLCYIIPGWGKIDGNGCATFGLYDGYATYCNGKLTFHKEDNNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P802</accession>
    <name>A0A218P802_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable Brix domain-containing ribosomal biogenesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_06040</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Probably involved in the biogenesis of the ribosome.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854163.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P802"/>
    <dbReference type="GeneID" id="33315811"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06040"/>
    <dbReference type="OrthoDB" id="117530at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10480">
        <property type="entry name" value="Probable brix-domain ribosomal biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00699">
        <property type="entry name" value="BriX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023548">
        <property type="entry name" value="Brix_dom_Rbsml_bgen_prot"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003053">
        <property type="entry name" value="PRK03972.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Brix" evidence="3">
        <location>
            <begin position="1"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ06912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="215" mass="25187" checksum="CDB7E4D6DE50E6E3" modified="2017-09-27" version="1">MMLITTSHRPTRRTRSFGHDLEKVFPNSLYLTRGKKTVQDLLMEAYDRNYERLLIVNVWKGNPLKMTFIKVDPEDWGYMGYLYLHGIKLQREMGYRDIRPIREEMPFVVTTAKRVGLDHVAFAQAFAELTGGTFVPRRERSLHGIADRYNTDVLGVIERHPRGMAVNFYRFDVDKENPVGPLISVKIWIMEDGRRWDYKEALGIKAQRRPGPSRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P826</accession>
    <name>A0A218P826_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADH dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06185</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854188.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P826"/>
    <dbReference type="GeneID" id="33315840"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06185"/>
    <dbReference type="OrthoDB" id="5740at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005012">
        <property type="entry name" value="PRK06411.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11995">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11995:SF14">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56770">
        <property type="entry name" value="HydA/Nqo6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase-like 20kDa subunit" evidence="1">
        <location>
            <begin position="25"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="193" mass="22538" checksum="B700B4D61105C654" modified="2017-09-27" version="1">MVDWRLFEPLFEYARKRSMWIVSFCTGCGGIEMPPLATSRYDFERFGIMPNPSPRMADLFLITGYVTPKTLKRIIITYEMQPDPKYVLAHGSCPLNGGIYWDGYNAIKHLDKYIPVDVAIAGCMPRPEAVMDGIQKMMELIETGKADGWKRYKENYEYYKKNQDELFGEGWREKTARRWIPWVLGKDKGVREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P863</accession>
    <name>A0A218P863_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854245.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P863"/>
    <dbReference type="GeneID" id="33315902"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06495"/>
    <dbReference type="OrthoDB" id="23833at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053629">
        <property type="entry name" value="NADPH_Sulfhydrogenase_Subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040833">
        <property type="entry name" value="sulfhyd_HydB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40447">
        <property type="entry name" value="ANAEROBIC SULFITE REDUCTASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40447:SF1">
        <property type="entry name" value="ANAEROBIC SULFITE REDUCTASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17179">
        <property type="entry name" value="Fer4_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="222"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="303"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="367" mass="43445" checksum="0B62AF900732D937" modified="2017-09-27" version="1">MRYVKLPKENTYTFLERLKDWGKLYAPVKISEKFYDFREIDDVRKVEFHYNRTIMPPKKFFFKPREKLFEFSIKEAEYRETIEDVEPFVLFGVHACDIFGLKIMDTIYLDELPDKYYKVRREKGIIIGISCMPDEYCFCNLRETDFADDGFDLFLHELPDGWLVRVGTPIGHRIVDKNLRLFKEVTTEDICNFREFENKRSNAFKYHEDWSNLRYLLELEMEHPMWDEQAAICLACGNCNTTCPTCRCYEVQDIVNLDGNTGYRERRWDSCQLRSHGLVAGNHNFRPTKKARFRNRYLCKNSYNEKLGISYCVGCGRCTYFCPAGISFVRNLRTIMGLEEKSCPPEITEEIPKKGFAYATEIRGEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P880</accession>
    <name>A0A218P880_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Cytochrome-c3 hydrogenase subunit gamma</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">A3L08_06490</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="10">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854244.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P880"/>
    <dbReference type="GeneID" id="33315901"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06490"/>
    <dbReference type="OrthoDB" id="35401at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06221">
        <property type="entry name" value="sulfite_reductase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.240.10">
        <property type="entry name" value="Dihydroorotate dehydrogenase, electron transfer subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012165">
        <property type="entry name" value="Cyt_c3_hydrogenase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037117">
        <property type="entry name" value="Dihydroorotate_DH_ele_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019480">
        <property type="entry name" value="Dihydroorotate_DH_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050353">
        <property type="entry name" value="PyrK_electron_transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054941">
        <property type="entry name" value="sulfhyd_HydG"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006220">
        <property type="entry name" value="PRK08345.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040835">
        <property type="entry name" value="sulfhyd_HydG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43513:SF1">
        <property type="entry name" value="ANAEROBIC SULFITE REDUCTASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43513">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE B (NAD(+)), ELECTRON TRANSFER SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10418">
        <property type="entry name" value="DHODB_Fe-S_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006816">
        <property type="entry name" value="Cyc3_hyd_g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00410">
        <property type="entry name" value="PHEHYDRXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 10" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="11">
        <location>
            <begin position="18"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR006816-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ06995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="295" mass="33271" checksum="AB642FBB641B7E2F" modified="2017-09-27" version="1">MSELVLPKEIMMPEENPYALHKAKVLKVYPLTEKEKLFLFRFGDPELAEKWTFRPGQFVQLTIPGVGEVPISICSSAMRRGFFELCIRKAGRVTTVIHRLKPGDTVLVRGPYGNGFPVDKWEGMDLLLIAAGLGTAPLRSVFLYAMDNRWKYGNITFINTARYGKDLLFYKELEAMKDLAEAENVKIIQSVTRDPDWPGLHGRPQNFIPEANTDPKNTAVAICGPPRMYKSVFEALINYGYKPENIFVTLERKMKCGIGKCGHCNVGTSTSWKYICKDGPVFTYFDIVSTPGLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P889</accession>
    <name>A0A218P889_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Deaminase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_06430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RutC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P889"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06430"/>
    <dbReference type="OrthoDB" id="371655at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019239">
        <property type="term" value="F:deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00448">
        <property type="entry name" value="YjgF_YER057c_UK114_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.40:FF:000001">
        <property type="entry name" value="L-PSP family endoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="entry name" value="RutC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006056">
        <property type="entry name" value="RidA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019897">
        <property type="entry name" value="RidA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00004">
        <property type="entry name" value="Rid family detoxifying hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803">
        <property type="entry name" value="2-IMINOBUTANOATE/2-IMINOPROPANOATE DEAMINASE RIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803:SF58">
        <property type="entry name" value="PROTEIN HMF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="YjgF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01094">
        <property type="entry name" value="UPF0076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ06984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="130" mass="14256" checksum="7ABCE34B90065F56" modified="2017-09-27" version="1">MVKKTVVYTENAPKPIGPYSQAIAVHNPDRLIFVSGQIPIDPETGEIVKGDIREQARQAIDNLVEVLEAAGATVDDVVKVTVYLSDMNDYAEFNEIYEEYFGHSKPARAVVEVSKLPKGVKVEIEAIAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8A0</accession>
    <name>A0A218P8A0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 9">Peptide chain release factor subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Translation termination factor aRF1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">prf1</name>
        <name evidence="11" type="ORF">A3L08_06295</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 9">Heterodimer of two subunits, one of which binds GTP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the eukaryotic release factor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ06960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854209.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8A0"/>
    <dbReference type="GeneID" id="33315862"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06295"/>
    <dbReference type="OrthoDB" id="1011at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000032">
        <property type="entry name" value="Eukaryotic peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.60:FF:000003">
        <property type="entry name" value="Peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.960.10:FF:000003">
        <property type="entry name" value="Peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.960.10">
        <property type="entry name" value="eRF1 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00424">
        <property type="entry name" value="Rel_fact_arch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024049">
        <property type="entry name" value="eRF1_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020918">
        <property type="entry name" value="Peptide_chain-rel_aRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004403">
        <property type="entry name" value="Peptide_chain-rel_eRF1/aRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03676">
        <property type="entry name" value="aRF1_eRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10113">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03463">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55481">
        <property type="entry name" value="N-terminal domain of eukaryotic peptide chain release factor subunit 1, ERF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="10">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ06960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="415" mass="47568" checksum="9DF0E950290941C6" modified="2017-09-27" version="1">MSHKSAEMYELKKKVEELKSYRGRATELVSLYIPAGYDINKVMQQLREEYGTAQNIKSKSTRKNVLGALERAMQHLKLYRQTPENGLALFVGNVSEQEGVSDIKLWAIVPPEPLKVRLYRCDQTFVTEPLEEMLRVKDAYGLITVEKNEATIGLLRGKRIEVIDELTSNVPGKTRAGGQSARRYERIREQETHEFMKRIAEHANNAFLPLLEKGELRGIIIGGPGPTKEEFIEKDYLHHELRKKIIGVVDISYSGEYGLKELVEKASDILKDHEAIKERHLIQNFFRHLVKDTGMITYGEREVRKALELGAVDTLLISEGYDRVRVHAKCNNCGWEEEKTMSEQEFHVYKKKLTHCPKCGSQNLSFEKWDVAEELIKMAEEAGSNVEVISLDTEEGQQFYKAFGGIGAFLRYKIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8D5</accession>
    <name>A0A218P8D5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">2-oxoglutarate synthase subunit KorA</fullName>
            <ecNumber evidence="4">1.2.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">2-ketoglutarate oxidoreductase alpha chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">2-oxoglutarate-ferredoxin oxidoreductase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">A3L08_06765</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">P-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2 oxidized [2Fe-2S]-[ferredoxin] + 2-oxoglutarate + CoA = succinyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17297"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="EC" id="1.2.7.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterotetramer of the KorA, KorB, KorC and KorD subunits.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.3" evidence="4"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854292.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8D5"/>
    <dbReference type="GeneID" id="33315956"/>
    <dbReference type="KEGG" id="tpaf:A3L08_06765"/>
    <dbReference type="OrthoDB" id="31112at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000022">
        <property type="entry name" value="2-oxoglutarate ferredoxin oxidoreductase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050722">
        <property type="entry name" value="Pyruvate:ferred/Flavod_OxRd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006412">
        <property type="entry name" value="PRK08659.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154:SF14">
        <property type="entry name" value="2-OXOGLUTARATE SYNTHASE SUBUNIT KORA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154">
        <property type="entry name" value="PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate flavodoxin/ferredoxin oxidoreductase pyrimidine binding" evidence="8">
        <location>
            <begin position="22"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate:ferredoxin oxidoreductase core" evidence="9">
        <location>
            <begin position="282"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00052359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00064882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00066947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00071398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00076968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00079587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ASJ07042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="382" mass="41979" checksum="89202A858E853001" modified="2017-09-27" version="1">MRYPFPVGKSDFIQGDEAIARAAILAGCRFYAGYPITPASEIFEAMALYMPLVDGVSIQMEDEIASIAAIIGASWAGAKAMTATSGPGFSLMQENIGYAIMTETPIVIVDVQRGGPSTGQPTLAAQGDIMQAIWGTHGDHSLIVLSPSTVQEAFDFTIRAFNLAEKYRTPVVLLTDAEIAHMRERVHIPNPDEIEIVNRKLPANEEEAKYPFGDIHGDGVPPMPVFGKGYRTYVTGLTHDERGRPKTVDAEVHERLIKRIIDKLECNKADIITYDAYELEDAEIAIVTTGIVSRSAVRAVKILRERGVKAGLLKLNTIWPFDFDMIEELAERVKKIYVPEMNLGQLYHLVKEGANGKAEVELISKIGGEVHTPMEIVERVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8J8</accession>
    <name>A0A218P8J8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF402 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854365.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8J8"/>
    <dbReference type="GeneID" id="33316034"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07155"/>
    <dbReference type="OrthoDB" id="85277at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.380.10">
        <property type="entry name" value="FomD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007295">
        <property type="entry name" value="DUF402"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035930">
        <property type="entry name" value="FomD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41271">
        <property type="entry name" value="DUF402 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41271:SF1">
        <property type="entry name" value="DUF402 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04167">
        <property type="entry name" value="DUF402"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159234">
        <property type="entry name" value="FomD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF402" evidence="1">
        <location>
            <begin position="11"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="167" mass="19581" checksum="EE1667217F25966F" modified="2017-09-27" version="1">MKRIHLTYRRIPNRVLERGDEVIADLGDVIVARSRFEGMLAPLKVNGVKVIDNGYGMVYFAFVGKNYDILKVYDEEGNFKGLYIDVLAYTKRKRDTIEMLDLFLDIFIFPSGEAVLLDEDELEMALNYGLIDRKTFEFAYSVARNILEKLKRDEFPPQIVWEYEWGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8U1</accession>
    <name>A0A218P8U1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">A3L08_07565</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854437.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8U1"/>
    <dbReference type="GeneID" id="33316116"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07565"/>
    <dbReference type="OrthoDB" id="87732at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025302">
        <property type="entry name" value="DrrA1/2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43335">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43335:SF4">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13732">
        <property type="entry name" value="DrrA1-3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="6"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ASJ07186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="309" mass="34914" checksum="DC356650C231A357" modified="2017-09-27" version="1">MSTQAITVENLTKSYGRFRAVDGLTFEVENGEVFGFLGPNGAGKTTTILSMLGIIIPDGGRIQILGMDMAREPLKIKERIGYLPENATVYGELTAWKNLEFFANFYRMSNAEKENRITELLKRVGLWEVRYRKAKTFSKGMKQRLLIAQTLINDPELLILDEPTSGLDPEGAHLVKEVIREAKAEGKTVFFSSHVLSEVEELSDRVGIIVRGKLKAVGPIGDIKRQFMELEGYEIKVETKEPLPEIGHEDITRLERVAPNRAIIFARSDIREWLSKNLSSRGITILSLEIEEPSLEDVFLKTIYGREEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8V3</accession>
    <name>A0A218P8V3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_07665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854454.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8V3"/>
    <dbReference type="GeneID" id="33316136"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07665"/>
    <dbReference type="OrthoDB" id="381338at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="185" mass="20751" checksum="64EDDBD75931F1DC" modified="2017-09-27" version="1">MGRCLIYYVGELKKPEEWVLLSEELKSMDIWPIQLYGPKDIAKVLKKLCMEKGSAVVVNLPGGFYAVPNGQIQESGNGKGPGDVKLTTVKDMIAKRLKGRVEEIIITSEKGFENFAKDLFEGRIKISPPWWKKVLMILAAVVAIVALLVHFGIELPELSGTQRIALKVLALLLILFEGWRRGYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P8W7</accession>
    <name>A0A218P8W7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Aspartate racemase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_07765</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aspartate/glutamate racemases family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854470.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P8W7"/>
    <dbReference type="GeneID" id="33316155"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07765"/>
    <dbReference type="OrthoDB" id="359449at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047661">
        <property type="term" value="F:amino-acid racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1860">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015942">
        <property type="entry name" value="Asp/Glu/hydantoin_racemase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001920">
        <property type="entry name" value="Asp/Glu_race"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018187">
        <property type="entry name" value="Asp/Glu_racemase_AS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004380">
        <property type="entry name" value="Asp_race"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00035">
        <property type="entry name" value="asp_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21198:SF7">
        <property type="entry name" value="ASPARTATE-GLUTAMATE RACEMASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21198">
        <property type="entry name" value="GLUTAMATE RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01177">
        <property type="entry name" value="Asp_Glu_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53681">
        <property type="entry name" value="Aspartate/glutamate racemase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00923">
        <property type="entry name" value="ASP_GLU_RACEMASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="232" mass="25623" checksum="58C7926F664611B5" modified="2017-09-27" version="1">MAERVIGILGGMGPLATADLFRRIVEKTPAKRDQDHPRIIIYNNPKIPDRTAFILGQGEDPRPELIASARKLEECGADFIIMPCNTAHFFAETIQRAIRIPLVSMIDETAKVVREMGLRKVGLLATDGTIKGLVYHRALLEHGVQIAVPDKTNQEKVMRGIYKGVKAGNLELGRKLLLEVARRLERRSDGIIAGCTEVSVALRQEDLSVPLIDPMDVIAEKAVKLALGLEDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P924</accession>
    <name>A0A218P924_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_08060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_198362107.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P924"/>
    <dbReference type="GeneID" id="60595939"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08060"/>
    <dbReference type="OrthoDB" id="1018at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="11922" checksum="1DC5AF1AC99D58DD" modified="2017-09-27" version="1">MSFTLELFDTPEIPKVLGEVKRVLKPGGRFGVVGMSKGEGLTVRLYEWLHERFPQYLDCRPIDLPEVVKGAGFVVVHEEEGRIFGLPVTIVIALKPKRNHHGEGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P936</accession>
    <name>A0A218P936_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Magnesium transporter MgtE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">A3L08_08070</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">P-4</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC41A transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854524.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P936"/>
    <dbReference type="GeneID" id="33316218"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08070"/>
    <dbReference type="OrthoDB" id="86118at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.20">
        <property type="entry name" value="SLC41 divalent cation transporters, integral membrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006667">
        <property type="entry name" value="SLC41_membr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036739">
        <property type="entry name" value="SLC41_membr_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045349">
        <property type="entry name" value="SLC41A1-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16228">
        <property type="entry name" value="DIVALENT CATION TRANSPORTER SOLUTE CARRIER FAMILY 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16228:SF7">
        <property type="entry name" value="SLC41A_MGTE INTEGRAL MEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01769">
        <property type="entry name" value="MgtE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161093">
        <property type="entry name" value="MgtE membrane domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="126"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="166"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="301"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="334"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="SLC41A/MgtE integral membrane" evidence="10">
        <location>
            <begin position="60"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="SLC41A/MgtE integral membrane" evidence="10">
        <location>
            <begin position="263"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ASJ07278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="394" mass="42801" checksum="4FB8A2C824C4575F" modified="2017-09-27" version="1">MSLAVGRNGSGRQSFIRDALLVSFPALVVCVFLDFFSGVFLGKFFTLIRTRYPIILVILPGLMGMRGNIFGALASRFSTMLYLGEMEPSLRDRKVMRNIFLSIVLSLIPVIFLWTVGAIKVGDPGVAVAVLLIVFMSTVYASLILGYSTALVSIIPFKKGLDPDSVAAPVITSVADLITVPLLVGFMLLYPHRSQFALMTAVAVFLLLLLGWRAKLTKRDIENTRELLMVVGGLALLSSISGSLLESYSEVIEKTLIFSVMYPMVLDTTGNIGSIIGAKTSTKIHLGELEGLFSRSILLEIGTYTALAFPLALIGNLISIGVVRFLFGKTVGIVLPFILLYPFIVFVAMWVAYFTAILGDRLGLDPDNVTVPTITTLADILSTLFVVLVARLMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P946</accession>
    <name>A0A218P946_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peroxiredoxin</fullName>
            <ecNumber evidence="9">1.11.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Thioredoxin peroxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Thioredoxin-dependent peroxiredoxin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">A3L08_07965</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8 9">Homodecamer. Pentamer of dimers that assemble into a ring structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="9">The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. Although the primary sequence of this enzyme is similar to those of the 1-Cys Prx6 enzymes, its catalytic properties resemble those of the typical 2-Cys Prxs and C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by thioredoxin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 9">Belongs to the peroxiredoxin family. Prx6 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="9"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854508.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P946"/>
    <dbReference type="GeneID" id="33316198"/>
    <dbReference type="KEGG" id="tpaf:A3L08_07965"/>
    <dbReference type="OrthoDB" id="6924at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03016">
        <property type="entry name" value="PRX_1cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1020.10:FF:000002">
        <property type="entry name" value="Peroxiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000011">
        <property type="entry name" value="Peroxiredoxin PRX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1020.10">
        <property type="entry name" value="Antioxidant, Horf6, Chain A, domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00401">
        <property type="entry name" value="Peroxiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050217">
        <property type="entry name" value="Peroxiredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022915">
        <property type="entry name" value="Peroxiredoxin_TDXH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045020">
        <property type="entry name" value="PRX_1cys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009668">
        <property type="entry name" value="PRK13189.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681:SF171">
        <property type="entry name" value="PEROXIREDOXIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681">
        <property type="entry name" value="THIOREDOXIN PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="1 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 9" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="6 9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="11">
        <location>
            <begin position="2"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="9">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity" evidence="10">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Interchain (with Cys-211); in linked form" evidence="9">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Alternate" evidence="9">
        <location>
            <begin position="205"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Interchain (with Cys-46); in linked form" evidence="9">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00064044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ07260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="216" mass="24747" checksum="5CC2752762852E4D" modified="2017-09-27" version="1">MVVIGEKFPEVEVNTTHGRIKLPDYFTEQGKWFVLFSHPADFTPVCTTEFYAMQKRAEEFRKLGVEPIGLSVDQVFSHLKWMEWIKENLGEEITFPVIADDRGELADKLGMIPSGSTHTARAVFVVDDKGTIRAIVYYPAEVGRDWDEILRLVKALKISTEKGVALPHKWPNNELIGDRVIVPPAGTVEQIKEREEAKAKGEIECYDWWFCHKKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P949</accession>
    <name>A0A218P949_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Membrane protein 6-pyruvoyl-tetrahydropterin synthase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">A3L08_08275</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854563.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P949"/>
    <dbReference type="GeneID" id="33316261"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08275"/>
    <dbReference type="OrthoDB" id="86324at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd03143">
        <property type="entry name" value="A4_beta-galactosidase_middle_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018776">
        <property type="entry name" value="Membrane_prot_PTPS-rel_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018580">
        <property type="entry name" value="Uncharacterised_YfhO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38454:SF1">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38454">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10131">
        <property type="entry name" value="PTPS_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="343"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="378"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="790"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane protein 6-pyruvoyl-tetrahydropterin synthase-related" evidence="2">
        <location>
            <begin position="70"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ASJ07317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="823" mass="92297" checksum="D985457F6FC3796F" modified="2017-09-27" version="1">MVEIRSARTREALFILSVFFLSLFVLRGFLSSGYPPSWGGDAYGHLFKIWKLMQGYSPWIEDWYGGYPFLRFYPPLSYFIGALLGKIASSAVTGYKLTVLLAILVGAISTRILLKELGFSDASSYLSGIVYALSVYHLRVLSPEGNFPRFFAINLAPLFILALLYITRKNWRYAVLSGLFLAAVGLAHHTLFVSFGLMVLFLLPYVWITRRNGVRDIALNLLIAGVTALLISSFWVLPFLLEKSNAHFLKENSIEYLFKFQSVRLRELLFHTSSWSFYQGLAFYMGIVGIVVLLAKGEKSERTLGAGLLGASLTAILLSLGYYGPTPFLNRLPLLDMIPPYRWLDSLSLAGATGVGALFEVLSGLIPQKIGSGAKRKAAAGILLAFLVVLSLSDIRLQVNSLKAEDFPGDYLAVLNYVGNDNSTGWRFYQPGLGMTQGSRVSWTPALAEKPSLDGWYRQGDPAYPQHSYLNYAIEKDPGFARKALRAYSMKYIITDENYRGYADIIRNLRSFGFEEIYSSGPFHLYRWSNFTFLQPKTGILVIGNWPFDLGVSYERGEYVDDYTDGLNEYSLVILNGYKYRDPLVWPKLEDYVKNGGTLVLNTFPSPDAEATRFGVKSVIVRVFGRANFTSSIYNVSAFSNFTYEGQPWTATAYYGDMTPLIKLENYTVLGYKNYGKGRVYFVGLNLPYHATYTDNGYEKGILEGLLAGYIKPPKVSYHVIDMGDGYIRLEFNLSRASTVVVSENYYPHWKGYVDGNKVEIKKNTEFGLMEVALPAGSHVLELKFKDPLSFLRCLGLISLALAILLLALSTGRASHRTWGRDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P964</accession>
    <name>A0A218P964_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">tRNA (guanine(10)-N(2))-dimethyltransferase</fullName>
            <ecNumber evidence="13">2.1.1.213</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">tRNA:G10 dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">A3L08_08385</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the adenosylmethionine-dependent methylation of the exocyclic amino group (N(2)) of guanosine at position 10 of various tRNAs. Acts via a two-step process that leads to the formation of either N(2)-monomethyl (m(2)G) or N(2)-dimethylguanosine (m(2)(2)G).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>guanosine(10) in tRNA + 2 S-adenosyl-L-methionine = N(2)-dimethylguanosine(10) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43124"/>
            <dbReference type="Rhea" id="RHEA-COMP:10355"/>
            <dbReference type="Rhea" id="RHEA-COMP:10358"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
            <dbReference type="EC" id="2.1.1.213"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the methyltransferase superfamily. Trm-G10 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.213" evidence="13"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854584.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P964"/>
    <dbReference type="GeneID" id="33316283"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08385"/>
    <dbReference type="OrthoDB" id="7080at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160101">
        <property type="term" value="F:tRNA (guanine(10)-N2)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160102">
        <property type="term" value="F:tRNA (guanine(10)-N2)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11715">
        <property type="entry name" value="THUMP_AdoMetMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000251">
        <property type="entry name" value="Putative RNA methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2130.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RlmKL-like_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005885">
        <property type="entry name" value="TrmG10"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01177">
        <property type="entry name" value="TIGR01177 family methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14911:SF21">
        <property type="entry name" value="N2-METHYLGUANOSINE TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14911">
        <property type="entry name" value="THUMP DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00507">
        <property type="entry name" value="N12N6MTFRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 17" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="16">
        <location>
            <begin position="42"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00066936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00082665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ASJ07337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="332" mass="37968" checksum="BD168D07891279D4" modified="2017-09-27" version="1">MLYLEILGNLPEMARDEVRAMLELAGGEIVGQDYLFLKIDADEKSFHYLNRLGLAHEYGELIVEADSIEELLEKAGEVEWPIKGTFKVDTETMANCRFNVLDLPRKLGAVIHAQGFKVNLSKPDTLVRVYCGERLYAGIRLKFFDPKDFEKRKAHHRPFFRPISLHPRVSRALVNLTKAKREILDPFMGAGGILMEAGLIGLKVYGVDIKPEMVEGAEMNLRHYGIKDYELKLGDATKLEELFPNKRFEAIATDPPYGTSATLAGRKRDELYRKALESMYNVLKPGGRLAIAFPADFDGRTEAERIGFKLLARYYQRVHKSLERYFYVFERP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P982</accession>
    <name>A0A218P982_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sugar-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_08190</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P982"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08190"/>
    <dbReference type="OrthoDB" id="30637at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14748">
        <property type="entry name" value="PBP2_UgpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050490">
        <property type="entry name" value="Bact_solute-bd_prot1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006061">
        <property type="entry name" value="SBP_1_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43649:SF30">
        <property type="entry name" value="ABC TRANSPORTER SUBSTRATE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43649">
        <property type="entry name" value="ARABINOSE-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01037">
        <property type="entry name" value="SBP_BACTERIAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="450" mass="50214" checksum="0A4D1296DF774E3E" modified="2017-09-27" version="1">MQKKPLFGILLLGVFILGVVASGCISGEKTSTATASQSGSTETGKVIELEFWHGIESPDNQKVLEDLVKKFEAEHPNIKVKVVNYGAQDKAVPKIMAAAQAGTPPDLVWLNPAQTGFFAEAGVLAPVEDFIKNDPSFNKDDIYAGLWELGTYKGKIYTAPFDTNNLAIYYNKELFKEAGLDPEAYMGKALTWDEFRGLAKKLTKDKDGDGEIDQYGFMVPFGRLEWTVWTWEVFLWSAGGELLSEDQSKPTFASDAGVKALQYWVDLVYKDKVAKLSEPNAGYKLDDFFAGRVAMTINGPWNYPVLKDQGWLEKTGVMLMPYDKRIATNIGGENLFIFKTTPEREQAAWEFAKFVMSPEFQVTWALKTGYLPVCKSALEDPEYKEFMEENPFIKIYVENLQYGKARPPVPQYPDMSAAIGEAIEAALLQKKDPATALKEAQAEVEPLFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9A6</accession>
    <name>A0A218P9A6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">6-carboxy-5,6,7,8-tetrahydropterin synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">A3L08_08555</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854613.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9A6"/>
    <dbReference type="GeneID" id="33316316"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08555"/>
    <dbReference type="OrthoDB" id="6529at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.10">
        <property type="entry name" value="6-pyruvoyl tetrahydropterin synthase/QueD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007115">
        <property type="entry name" value="6-PTP_synth/QueD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038418">
        <property type="entry name" value="6-PTP_synth/QueD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589:SF7">
        <property type="entry name" value="6-PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589">
        <property type="entry name" value="PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01242">
        <property type="entry name" value="PTPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ASJ07366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="153" mass="17690" checksum="60D9CE9300051C82" modified="2017-09-27" version="1">MAFRISERKIGWHKDFDSSHFLALPYESKCLRIHGHTYNVDVEIWGDLNENGMIFDFNHLSNLVKLLDHRILVSENWVAESGGGRLVIEKNGKRIELPENEAVVLNKPNVTAEYIAEWFAEKIAEKAGDNVRKIRVKVWEDPRSYAEVALERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9B4</accession>
    <name>A0A218P9B4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_08515</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854606.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9B4"/>
    <dbReference type="GeneID" id="33316309"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08515"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="72" mass="8235" checksum="7658056E371613B7" modified="2017-09-27" version="1">MPRGMGRGYGRGRGFYPLGGAYGIIELLILVGILYFLIKLFLVALPYALGLLVLLIIRELIRPRPRTWFRPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9C4</accession>
    <name>A0A218P9C4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_08675</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854632.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9C4"/>
    <dbReference type="GeneID" id="33316341"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08675"/>
    <dbReference type="OrthoDB" id="140416at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008725">
        <property type="term" value="F:DNA-3-methyladenine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005019">
        <property type="entry name" value="Adenine_glyco"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052891">
        <property type="entry name" value="DNA-3mA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30037">
        <property type="entry name" value="DNA-3-METHYLADENINE GLYCOSYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30037:SF4">
        <property type="entry name" value="DNA-3-METHYLADENINE GLYCOSYLASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03352">
        <property type="entry name" value="Adenine_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="441" mass="51412" checksum="7B53647B8ED0181E" modified="2017-09-27" version="1">MGVTAFIVVGNRPGFTPGIPNPGYLLLLYENDRPAWELIPLYPELRKDLGNSKILIPSIENMLEDALLMIGIYVVKDEELIEKARRIFRDSLERRIEIYAFDRREVEDLRSIARRKLQRYDIGLIIAPGEDSTILGQLEVLREYGDLWYQVNLPDKKLSNTPGFFDEIPLQEHSDSPRWEYTEAFWKILNKLEEKSIDKKKFRRGIEDFNSYRERFRKMSDDEIYSTLVQVVFYSGMNAKTVSKKMPTILKYLGDFRKVAEYTEEDIRRIMDDRDMIKNLKKIQACIHNAKEFQRIVESHGSFVNYLESFEVDPTNYRDIKTKLVPELMKRFKGIGKVTVYHFLMDLGFGVMKPDRTILRLFHRLGWLPTSDPTAENIERTIKICSEISSETGFWIRAVDITLVAFCQEGGNQGLGIPAGICTRNPKCLECPLRERCVLHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9P5</accession>
    <name>A0A218P9P5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">A3L08_09075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854704.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9P5"/>
    <dbReference type="GeneID" id="33316421"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09075"/>
    <dbReference type="OrthoDB" id="94668at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ASJ07460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="236" mass="26050" checksum="3946F9E078D7D52A" modified="2017-09-27" version="1">MERTSKGLVVVFLAGMILGILTSYAFIHDSGPSPEVRTCPIVNHEVLMEYLVRASPTAGGEATAKAVESAYLEQRSPNYPLELFREMYRDVPGRKEFLGLSYIFSNDTLSYGKFTLTNETPPRFEFASPAPGMSQRVDGAGIIASRWAVIFVSLYDFGGMNEVEELYLTVPEGWKLEYYGGTGDLLPGTVLRVRFSSTKRNNYVVLAFSTGGVLDPLDLEVTVNGDTYRLVHSSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218P9W4</accession>
    <name>A0A218P9W4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription regulator AsnC/Lrp ligand binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">A3L08_09720</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">P-4</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218P9W4"/>
    <dbReference type="KEGG" id="tpaf:A3L08_09720"/>
    <dbReference type="OrthoDB" id="94259at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ASJ07577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="93" mass="10673" checksum="5779715C07D7F30E" modified="2017-09-27" version="1">MRKIEAILLVWGVRDEVLEKLPLEGHWLYGEYDFIAKIELRDEFEMEQFAEELRHLIHGGTFKLMPIALSTIKNGVEGSETVSMLESLKVSAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PA01</accession>
    <name>A0A218PA01_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 11">7-cyano-7-deazaguanine synthase</fullName>
            <ecNumber evidence="9 11">6.3.4.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">7-cyano-7-carbaguanine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Archaeosine biosynthesis protein QueC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">PreQ(0) synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">queC</name>
        <name evidence="12" type="ORF">A3L08_00700</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">P-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 11">Catalyzes the ATP-dependent conversion of 7-carboxy-7-deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>7-carboxy-7-carbaguanine + NH4(+) + 2 ATP = 7-cyano-7-carbaguanine + 2 AMP + 2 diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:27982"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 11">Belongs to the QueC family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.20" evidence="9 11"/>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_088854849.1">
        <property type="nucleotide sequence ID" value="NZ_CP015102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PA01"/>
    <dbReference type="GeneID" id="33314744"/>
    <dbReference type="KEGG" id="tpaf:A3L08_00700"/>
    <dbReference type="OrthoDB" id="6532at2157"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01995">
        <property type="entry name" value="QueC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01633">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018317">
        <property type="entry name" value="QueC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00364">
        <property type="entry name" value="7-cyano-7-deazaguanine synthase QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42914">
        <property type="entry name" value="7-CYANO-7-DEAZAGUANINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42914:SF1">
        <property type="entry name" value="7-CYANO-7-DEAZAGUANINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06508">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006293">
        <property type="entry name" value="ExsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ASJ07604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="239" mass="26384" checksum="536A38305FBF2EC7" modified="2017-09-27" version="1">MKRAVVLFSGGLDSTACLYWAKKNYDEVIMLTVNYGSNEEKVTNRVAEFFSKELGVPLKIARLDFLEEFSKLRGTTLVGGETPKVTAQELEDMSVAQETAKSVWVPARNVVLISVAASLLDALGGGDIIVGFNAEEGATFPDNTPEFAERMNEMLRYGTMAEVRVVAPLINLDKKGIAKLLKELNAKYEYSNSCYMPKGFTEDGKPIHCGECESCVRRHRGLIEGIGEDRTVYAVEPEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-09-27" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A218PAC6</accession>
    <name>A0A218PAC6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ADP-ribosylglycohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">A3L08_08680</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermococcus pacificus</name>
        <dbReference type="NCBI Taxonomy" id="71998"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Thermococci</taxon>
            <taxon>Thermococcales</taxon>
            <taxon>Thermococcaceae</taxon>
            <taxon>Thermococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete genome sequence of Thermococcus pacificus type strain P4.</title>
            <authorList>
                <person name="Oger P.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">P-4</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="3">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ADP-ribosylglycohydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP015102">
        <property type="protein sequence ID" value="ASJ07690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A218PAC6"/>
    <dbReference type="KEGG" id="tpaf:A3L08_08680"/>
    <dbReference type="OrthoDB" id="114878at2157"/>
    <dbReference type="Proteomes" id="UP000197418">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4080.10">
        <property type="entry name" value="ADP-ribosylation/Crystallin J1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050792">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005502">
        <property type="entry name" value="Ribosyl_crysJ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036705">
        <property type="entry name" value="Ribosyl_crysJ1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222">
        <property type="entry name" value="ADP-RIBOSYLGLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222:SF24">
        <property type="entry name" value="ADP-RIBOSYLHYDROLASE ARH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03747">
        <property type="entry name" value="ADP_ribosyl_GH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101478">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR605502-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ASJ07690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000197418"/>
        </source>
    </evidence>
    <sequence length="318" mass="35920" checksum="8F8F34BCA8D9D47C" modified="2017-09-27" version="1">MGNEPDLESRLKGGLWGVVVGDAFGFPFQFWSRERMREEYPNPEDIPMLDGLWSDDSSLTLATAHALKDGYSLERIAQNFISWYYDGEFTPRGHAFDEGITTSRAIERLAKGVFPLNAGGRGERDNGNGSLMRILPVAYYAYFKMKNLEERLQMIHEVSMLTHAHPRSLVGCGIYSMVVWNILSGLDKFDAYSEAVELAREVYSMEPFSRELEHYERVLSGKIHKFAESEIRGSGYVVHTLEAALWAFLRNNSFEDAIREVVSLAEDTDTTGAVVGGLAGTYYGFEAIPGEWLGKIEARELIEEIINDFVFSLIEARR</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>